Journal of investigational allergology & clinical immunology最新文献

{"title":"MRGPRX2 and Immediate Drug Hypersensitivity: Insights From Cultured Human Mast Cells.","authors":"J Elst,&nbsp;V Sabato,&nbsp;M A Faber,&nbsp;C H Bridts,&nbsp;C Mertens,&nbsp;M Van Houdt,&nbsp;A L Van Gasse,&nbsp;M M Hagendorens,&nbsp;V Van Tendeloo,&nbsp;M Maurer,&nbsp;D Campillo-Davo,&nbsp;J P Timmermans,&nbsp;I Pintelon,&nbsp;D G Ebo","doi":"10.18176/jiaci.0557","DOIUrl":"https://doi.org/10.18176/jiaci.0557","url":null,"abstract":"<p><strong>Background and objectives: </strong>Mast cell (MC) degranulation via activation of the Mas-related G protein-coupled receptor X2 (MRGPRX2) plays a key role in immediate drug hypersensitivity (IDH). However, data in humans are limited to observations in specific cell lines. Objective: To study the usefulness of silencing MRGPRX2 in human MCs with the aim of further unveiling the MRGPRX2 pathway in IDH.</p><p><strong>Methods: </strong>MCs were cultured from CD34+ progenitor cells obtained from peripheral blood (PBCMCs) and incubated with substance P (as a positive control), rocuronium, moxifloxacin, morphine, or amoxicillin. Immunophenotyping of the cells included flow cytometry and microscopy analyses of the expression of CD117, CD203c, and MRGPRX2. Intracellular calcium was measured using Fluo-4. Degranulation was analyzed by quantifying CD63 expression. For MRGPRX2 silencing, MCs were electroporated with Dicer small interference RNAs.</p><p><strong>Results: </strong>Incubation of MCs with substance P, morphine, and moxifloxacin increased intracellular calcium levels and triggered MC degranulation, which, for the drugs, is almost completely abolished by selective MRGPRX2 silencing. Despite an increase in intracellular calcium in MRGPRX2+ cells, incubation with nontoxic concentrations of rocuronium does not result in degranulation of PBCMCs. Amoxicillin has no effect on PBCMCs.</p><p><strong>Conclusion: </strong>The use of MRGPRX2 silencing in human MCs can provide important insights into the role of MRGPRX2 in the pathogenesis of IDH. As induction of calcium signals does not necessarily translate into a secretory response, measurement of the degranulation reaction seems more meaningful in the context of drug testing.</p>","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"489-499"},"PeriodicalIF":7.2,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.18176/jiaci.0557","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38210068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Associated With Asthma Control in 121 Preschool Children.","authors":"P Leiria-Pinto,&nbsp;P Carreiro-Martins,&nbsp;I Peralta,&nbsp;J Marques,&nbsp;E Finelli,&nbsp;C Alves,&nbsp;J Belo,&nbsp;M Alves,&nbsp;A L Papoila,&nbsp;N Neuparth","doi":"10.18176/jiaci.0630","DOIUrl":"https://doi.org/10.18176/jiaci.0630","url":null,"abstract":"<p><strong>Background and objectives: </strong>Data on risk factors for uncontrolled asthma in preschool children are controversial. Objective: This study aims to explore the association between clinical and functional parameters and the lack of asthma control in preschool children.</p><p><strong>Methods: </strong>Children aged 3-5 years with asthma and healthy controls were recruited. A questionnaire was used to identify potential risk factors for uncontrolled asthma, as defined by the Global INitiative for Asthma criteria. Lung function and bronchial reversibility were evaluated through impulse oscillometry and spirometry. Adjusted odds ratios were estimated based on multivariable generalized additive regression models. The discriminative ability of the models was measured by the area under the receiver operating characteristic curve (AUC).</p><p><strong>Results: </strong>The study population comprised 121 children (107 with asthma and 14 healthy controls). Fifty-three patients (50%) had uncontrolled asthma. After adjustment, the variables associated with an increased risk of lack of control were as follows: \"More than 3 flare-ups in the last 12 months\", \"Moderate to severe rhinitis\", and \"Relative variation in postbronchodilator FVC and FEV1\". The AUC of the final models that included variation in FVC or FEV1 were 0.82 and 0.81, respectively. The R5-20, R5-20%, and AX z-score values of the healthy group were lower than those of children with asthma.</p><p><strong>Conclusion: </strong>In preschool children, clinical and functional parameters are associated with uncontrolled asthma. More studies are needed to confirm the usefulness of impulse oscillometry.</p>","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"471-480"},"PeriodicalIF":7.2,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.18176/jiaci.0630","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38177324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaphylaxis due to Perioperative Intravenous Lidocaine.","authors":"S K Carrión Sari,&nbsp;A Lezaun,&nbsp;C Colas Sanz","doi":"10.18176/jiaci.0626","DOIUrl":"https://doi.org/10.18176/jiaci.0626","url":null,"abstract":"","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"164-165"},"PeriodicalIF":7.2,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.18176/jiaci.0626","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38074562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and Effectiveness of Dupilumab in Prurigo Nodularis.","authors":"C Romano","doi":"10.18176/jiaci.0550","DOIUrl":"https://doi.org/10.18176/jiaci.0550","url":null,"abstract":"","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"162-163"},"PeriodicalIF":7.2,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.18176/jiaci.0550","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38079167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SEAIC Specialty Forum: Analysis of the Current Situation of Allergology in Spain and Outlook for the Future.","authors":"J Sastre,&nbsp;A Valero Santiago,&nbsp;J Montoro Lacomba,&nbsp;S Quirce,&nbsp;C Vidal Pan,&nbsp;I Dávila González,&nbsp;J M Olaguibel Rivera,&nbsp;M J Torres Jaén,&nbsp;V Rodríguez Vázquez,&nbsp;D Antolin Amerigo,&nbsp;P Ojeda Fernández","doi":"10.18176/jiaci.0473","DOIUrl":"https://doi.org/10.18176/jiaci.0473","url":null,"abstract":"<p><strong>Background and objectives: </strong>Allergology has been a recognized medical specialty in Spain, with fully defined aims and competencies for more than 4 decades. However, in recent years, its visibility seems to have decreased somewhat. Objectives: To identify which specific factors have contributed to the waning of the importance of the specialty and find tangible solutions to consolidate its place as a front-line medical specialty.</p><p><strong>Material and methods: </strong>An online population survey comprising 60 items of interest was prepared. The degree of agreement and the level of satisfaction with each item were assessed, and implementable initiatives in the short, medium, and long terms were defined in order to provide solutions to the issues identified.</p><p><strong>Results: </strong>The survey was completed by a total of 167 specialists with an average of 18 years' experience. Most were from public reference hospitals, and 29.3% were heads of department. The line of action for which a good degree of agreement was achieved was to promote the inclusion of an allergist in multidisciplinary teams. The priority lines of action were to improve undergraduate and graduate training in allergology and specialized nursing, to identify curricula in Spain, and to develop robust teaching projects.</p><p><strong>Conclusions: </strong>The results revealed a high degree of homogeneity between professionals. The basic pillars highlighted were as follows: quality training, knowledge, and research in immunotherapy; an innovative portfolio of services endorsed by clinical practice guidelines; and presence in multidisciplinary teams and relevant hospital committees.</p>","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"120-131"},"PeriodicalIF":7.2,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.18176/jiaci.0473","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37454205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Descriptive Study of Job Opportunities in the Allergy Field.","authors":"T Posadas Miranda,&nbsp;M J Rial Prado,&nbsp;A Meijide Calderón,&nbsp;I Fernández de Alba Porcel,&nbsp;G Zambrano Ibarra","doi":"10.18176/jiaci.0631","DOIUrl":"https://doi.org/10.18176/jiaci.0631","url":null,"abstract":"Sección Alergología Hospital General Universitario Reina Sofía Avda. Intendente Jorge Palacios, 1 30003 Murcia E-mail: juanc.miralles@carm.es 5. McDonald VM, Vertigan AE, Gibson PG. How to set up a severe asthma service. Respirology. 2011;16:900-11. 6. https://www.gemasma.com/sites/default/files/2020-05/ GEMA_50_ok_.pdf; Last accessed 10.05.2020. 7. Davila I, Quirce S, Olaguibel JM. Selection of Biologics in Severe Asthma: A Multifaceted Algorithm. J Investig Allergol Clin Immunol. 2019;29:325-8. 8. Morel F, Ojanguren I, Cordovilla R, Urrutia I, Aguero R, Guerra J, et al. Two short interventions to reduce health care requirements in asthma patients. A multicentre controlled study (ASTHMACAP II). Med Clin. 2014;142:348-54. Descriptive Study of Job Opportunities in the Allergy Field","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"184-186"},"PeriodicalIF":7.2,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38177328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phospholipase A2 Triggers Anaphylaxis to Snake Venom by Repeated Skin Sensitization: A Case Report.","authors":"K Swiontek,&nbsp;S Planchon,&nbsp;M Ollert,&nbsp;F Eyer,&nbsp;J Fischer,&nbsp;C Hilger","doi":"10.18176/jiaci.0545","DOIUrl":"https://doi.org/10.18176/jiaci.0545","url":null,"abstract":"1. Blanca-Lopez N, Perez-Alzate D, Andreu I, Doña I, Agundez JA, Garcıa-Martın E, et al. Immediate hypersensitivity reactions to ibuprofen and other arylpropionic acid derivatives. Allergy. 2016;71:1048-56. 2. Ortega N, Doña I, Moreno E, Audicana MT, Barasona MJ, BergesGimeno MP, et al. Practical guidelines for diagnosing hypersensitivity reactions to nonsteroidal anti-inflammatory drugs. J Investig Allergol Clin Immunol. 2014;24:308-23. 3. Doña I, Barrionuevo E, Blanca-López N, Torres MJ, Fernández TD, Canto G, et al. Trends in hypersensitivity drugs reactions: more drugs, more response patterns, more heterogeneity. J Investig Allergol Clin Immunol. 2014;24:143-53. 4. Fernández Sánchez FJ, Blanca Gómez M, Jerez Domínguez J, Rodríguez Fernández F, Martín Casañez E. Reacciones cutáneas graves producidas por fármacos: eritema exudativo multiforme, síndrome de Lyell, síndrome de hipersensibilidad a fármacos. In: Peláez Hernández A, Dávila González IJ, Editors. Tratado de Alergología II. Majadahonda (Madrid). Ergon, 2007; 1515-24. 5. Doña I, Blanca-Lopez N, Torres MJ, Garcia-Campos J, GarciaNunez I, Gomez F, et al. Drug hypersensitivity reactions: response patterns, drug involved, and temporal variations in a large series of patients. J Investig Allergol Clin Immunol. 2012;22:363-71. 6. Lobera Labairu T, Padial Vilchez MA, Guerrero García MA, Audicana Berasategui MT, García Abujeta JL. Concentraciones de principios activos y excipientes empleados para la realización de pruebas cutáneas y epicutáneas. In: Dávila González IJ, Jáuregui Presa I, Olaguibel Rivera JM, Zubeldía Ortuño JM, Editors. Tratado de Alergología IV. Majadahonda (Madrid). Ergon, 2016; 1679-86. 7. Pérez-Alzate D, Cornejo-García JA, Pérez-Sánchez N, Andreu I, García-Moral A, Agúndez JA, et al. Immediate Reactions to More Than 1 NSAID Must Not Be Considered CrossHypersensitivity Unless Tolerance to ASA Is Verified. J Invest Allergol Clin Immunol. 2017;27(1):32-9. 8. Himly M, Jahn-Schmid B, Pittertschatscher K, Bohle B, Grubmayr K, Ferreira F, et al. IgE-mediated immediate-type hypersensitivity to the pyrazolone drug propyphenazone. J Allergy Clin Immunol. 2003;111:882-8. 9. Mahboob A, Haroon TS. Drugs causing fixed eruptions: a study of 450 cases. Int J Dermatol. 1998;37:833-8. Phospholipase A2 Triggers Anaphylaxis to Snake Venom by Repeated Skin Sensitization: A Case Report","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"175-177"},"PeriodicalIF":7.2,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.18176/jiaci.0545","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38210067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe Asthma Units Accredited by the Spanish Society of Allergology and Clinical Immunology (SEAIC): Experience and Future.","authors":"J Delgado Romero,&nbsp;J C Miralles López,&nbsp;M Álvarez Puebla,&nbsp;E Fernández Ibáñez,&nbsp;A Habernau Mena","doi":"10.18176/jiaci.0618","DOIUrl":"https://doi.org/10.18176/jiaci.0618","url":null,"abstract":"","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"182-184"},"PeriodicalIF":7.2,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38177329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe Delayed Hypersensitivity Reaction to Abiraterone Acetate.","authors":"A Lainez-Nuez,&nbsp;J Crespo,&nbsp;B Noguerado-Mellado,&nbsp;M Tejero-Alcalde,&nbsp;P Tornero,&nbsp;P Rojas-Pérez-Ezquerra","doi":"10.18176/jiaci.0560","DOIUrl":"https://doi.org/10.18176/jiaci.0560","url":null,"abstract":"J Investig Allergol Clin Immunol 2021; Vol. 31(2): 162-181 © 2021 Esmon Publicidad Prior to Desensitization in New Drug Hypersensitivity: Chemotherapeutics and Biologicals. Curr Treat Options Allergy. 2020;7:1-13. 7. Castells M. Drug allergy: Veni, vidi, vici-come, understand, and delabel, avoid, or desensitize. Ann Allergy Asthma Immunol. 2019;123:1-2. 8. Berges-Gimeno MP, Carpio-Escalona LV, Longo-Muñoz F, Bernal-Rubio L, Lopez-Gonzalez P, Gehlhaar P, et al. Does Rapid Drug Desensitization to chemotherapy affect survival outcomes? J Investig Allergol Clin Immunol. 2020;30(4);25463. 9. Sloane D, Govindarajulu U, Harrow-Mortelliti J, Barry W, Hsu FI, Hong D, et al. Safety, costs, and efficacy of rapid drug desensitizations to chemotherapy and monoclonal antibodies. J Allergy Clin Immunol Pract. 2016;4:497-504. 10. Brown SG. Clinical features and severity grading of anaphylaxis. J Allergy Clin Immunol. 2004;114:371-6. 11. Brockow K, Romano A, Blanca M, Ring J, Pichler W, Demoly P. General considerations for skin test procedures in the diagnosis of drug hypersensitivity. Allergy. 2002;57:45-51. 12. Lopez-Gonzalez P, Madrigal-Burgaleta R, Carpio-Escalona LV, Bernal-Rubio L, Guerra E, Berges-Gimeno MP, et al. Assessment of antihistamines and corticosteroids as premedications in rapid drug desensitization to paclitaxel: outcomes in 155 procedures. J Allergy Clin Immunol Pract. 2018;6:1356-62. 13. Solano-Solares E, Madrigal-Burgaleta R, Carpio-Escalona LV, Bernal-Rubio L, Berges-Gimeno MP, Alvarez-Cuesta E. Chemotherapy in mastocytosis: administration issues, hypersensitivity, and rapid drug desensitization. J Investig Allergol Clin Immunol. 2017;27:315-7. 14. Ureña-Tavera A, Zamora-Verduga M, Madrigal-Burgaleta R, Angel-Pereira D, Berges-Gimeno MP, Alvarez-Cuesta E. Hypersensitivity reactions to racemic calcium folinate (leucovorin) during FOLFOX and FOLFIRI chemotherapy administrations. J Allergy Clin Immunol. 2015;135:1066-7. 15. Madrigal-Burgaleta R, Berges-Gimeno MP, Angel-Pereira D, Ferreiro-Monteagudo R, Guillen-Ponce C, Alvarez-Cuesta E, et al. Hypersensitivity and desensitization to antineoplastic agents: outcomes of 189 procedures with a new short protocol and novel diagnostic tools assessment. Allergy. 2013;68:853-61.","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"168-170"},"PeriodicalIF":7.2,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.18176/jiaci.0560","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38177323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Affecting the Success of Step-up Therapy in Patients With Moderate-Severe Asthma: A Real-Life Study.","authors":"J Delgado,&nbsp;E Martinez-Moragón,&nbsp;T Fernández-Sánchez","doi":"10.18176/jiaci.0470","DOIUrl":"https://doi.org/10.18176/jiaci.0470","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Introduction: Clinical practice guidelines recommend regular adjustment of treatment to achieve control of asthma. A step-up approach based on the degree of disease control should be followed. Objective: To perform a real-life analysis of the factors that affect the success or failure of this therapeutic strategy and of the criteria applied by clinicians when applying a step-up approach in a representative sample of patients diagnosed with moderate-severe asthma.</p><p><strong>Material and methods: </strong>We performed a multicenter retrospective cohort study involving 226 Spanish specialist physicians (98 allergologists, 127 pulmonologists, and 1 family physician). We included 1254 patients (787 women) diagnosed with moderate-severe asthma who underwent step-up therapy during 2016.</p><p><strong>Results: </strong>Step-up was successful in 44% of cases. The factors associated with success were presence of <2 comorbid conditions, lower grade of severity and therapy step before modification, absence of exacerbations during the previous year, fewer daytime/nighttime symptoms, and limitation in activities, as well as type of inhaled corticosteroid/ long-acting β₂-agonist combination after modification, lower body mass index, and higher fractional exhaled nitric oxide. An early increase in the maintenance dose once a lack of control was detected (≤3 months, >3 to ≤6 months, >6 to ≤12 months) was more likely to be successful.</p><p><strong>Conclusion: </strong>The factors that determine whether or not this therapeutic strategy manages to control asthma are time since onset of clinical impairment, previous grade of severity, number of comorbid conditions, previous exacerbations, and frequency of symptoms.</p>","PeriodicalId":520676,"journal":{"name":"Journal of investigational allergology & clinical immunology","volume":" ","pages":"145-150"},"PeriodicalIF":7.2,"publicationDate":"2021-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.18176/jiaci.0470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37454206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}