Journal of fungi (Basel, Switzerland)最新文献

{"title":"Subunit C of V-ATPase-VmaC Is Required for Hyphal Growth and Conidiation in <i>A. fumigatus</i> by Affecting Vacuolar Calcium Homeostasis and Cell Wall Integration.","authors":"Congcong Sun,&nbsp;Xingyue Li,&nbsp;Yuanwei Zhang,&nbsp;Ling Lu","doi":"10.3390/jof8111219","DOIUrl":"https://doi.org/10.3390/jof8111219","url":null,"abstract":"<p><p><i>Aspergillus fumigatus</i> is a widespread airborne fungal pathogen in humans. However, the functional genes in <i>A. fumigatus</i> that may contribute to its pathogenesis have not yet been fully identified. Vacuolar H⁺-ATPase is universal in eukaryotic organisms but exhibits specific roles in various species. Here, we identified VmaC as a putative subunit of vacuolar H⁺-ATPase in <i>A. fumigatus</i> that is widely conserved through evolution. The C-terminal hydrophobic domain of VmaC plays a critical role in its vacuolar localization and growth and conidiation. Deletion or turn-off of VmaC encoding gene-<i>AfvmaC</i> expression is not lethal but leads to a very sick and tiny colony phenotype, which is different from that of yeast with conditional <i>ScvmaC</i> defects. Furthermore, we found that <i>AfvmaC</i> not only participates in maintaining calcium homeostasis and vacuolar acidity but is also involved in cell wall integration pathway regulation, highlighting the importance of the vacuole as a storage organelle associated with many aspects of cellular homeostasis. This study indicates that fungal VmaC is relatively conserved. When compared to that in model yeasts, VmaC in <i>A. fumigatus</i> is required for hyphal growth and conidiation, suggesting that specific motifs in VmaC might be functioned in <i>Aspergilli</i>.</p>","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9699406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40484731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Investigation about <i>Aspergillus</i> spp. Spread in Umbrian Avian Farms.","authors":"Deborah Cruciani,&nbsp;Silvia Crotti,&nbsp;Carmen Maresca,&nbsp;Ivan Pecorelli,&nbsp;Emanuela Verdini,&nbsp;Marinella Rodolfi,&nbsp;Eleonora Scoccia,&nbsp;Sara Spina,&nbsp;Andrea Valentini,&nbsp;Francesco Agnetti","doi":"10.3390/jof8111213","DOIUrl":"https://doi.org/10.3390/jof8111213","url":null,"abstract":"<p><p>Among the fungi responsible for deep mycosis, the genus <i>Aspergillus</i> plays a predominant role both in human and veterinary medicine. From a \"One Health\" perspective, infections by <i>Aspergillus</i> spp. often represent a public health problem linked to specific occupational categories that could have a greater risk of inhaling spores and developing any respiratory disease. This preliminary investigation allowed to acquire information about the spread of <i>Aspergillus</i> spp. in avian livestock of the Umbria region (Central Italy), their sensitivity to antifungals, and the presence of mycotoxins in the considered farms. Environmental, feed, animal, and human samples were collected for mycological investigations; chemical analyses were also performed in feed samples. Moreover, prevalence estimated of the fungal isolates were provided for each individual farm sampled. Direct fungal identification was possible in 298 out of the 559 total samples; 162 of the samples were positive for <i>Aspergillus</i> spp. Mycotoxins were detected in 5 out of the 21 feed samples collected. All the aspergilli tested for antifungal susceptibility were resistant to fluconazole. The results obtained show how much the genus <i>Aspergillus</i> is widespread in the investigated farms; therefore, the poultry livestock represents a favorable environment for the maintenance and spread of fungal spores and their potential transmission to animals and humans.</p>","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9698509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40482852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flavonoids Modulate <i>Aspergillus flavus</i> Proliferation and Aflatoxin Production.","authors":"Lina Castano-Duque,&nbsp;Matthew D Lebar,&nbsp;Carol Carter-Wientjes,&nbsp;David Ambrogio,&nbsp;Kanniah Rajasekaran","doi":"10.3390/jof8111211","DOIUrl":"https://doi.org/10.3390/jof8111211","url":null,"abstract":"<p><p>Aflatoxins are carcinogenic mycotoxins produced by <i>Aspergillus flavus</i>. They contaminate major food crops, particularly corn, and pose a worldwide health concern. Flavonoid production has been correlated to resistance to aflatoxin accumulation in corn. The effects of flavonoids on fungal proliferation and aflatoxin production are not well understood. In this study, we performed bioassays, fluorescence and scanning electron microscopy, and total antioxidant analysis to determine the effects of three flavonoids (apigenin, luteolin, and quercetin) on proliferation and aflatoxin production in <i>A. flavus</i> NRRL 3357. Results showed that concentrations of apigenin and luteolin modulated fungal proliferation and aflatoxin production in a dose-dependent manner, leading to inhibition or promotion of proliferation and toxin production. Microscopy studies of fungi exposed to flavonoids showed mycelial cell wall disruption, abnormal cell wall invaginations, and tears. Fluorescent enhancement of apigenin and luteolin using Naturstoff reagent A showed that these chemicals localized in sphere-like structures on the mycelia surface. Fungi exposed to low concentrations of apigenin, luteolin, and quercetin lowered the total antioxidant capacity in the environment compared to controls. Our results indicate that flavonoids disrupt cell wall integrity and may localize in vesicle-like structures. We hypothesize that flavonoids could act as potential signaling molecules at low concentrations and change the oxidative state of the microenvironment, either or both of which may lead to reduction of fungal proliferation and aflatoxin production.</p>","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40482850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal Strains with Identical Genomes Were Found at a Distance of 2000 Kilometers after 40 Years.","authors":"Qili Zhu,&nbsp;Yang Lin,&nbsp;Xueliang Lyu,&nbsp;Zheng Qu,&nbsp;Ziyang Lu,&nbsp;Yanping Fu,&nbsp;Jiasen Cheng,&nbsp;Jiatao Xie,&nbsp;Tao Chen,&nbsp;Bo Li,&nbsp;Hui Cheng,&nbsp;Weidong Chen,&nbsp;Daohong Jiang","doi":"10.3390/jof8111212","DOIUrl":"https://doi.org/10.3390/jof8111212","url":null,"abstract":"<p><p>Heredity and variation are inherent characteristics of species and are mainly reflected in the stability and variation of the genome; the former is relative, while the latter is continuous. However, whether life has both stable genomes and extremely diverse genomes at the same time is unknown. In this study, we isolated <i>Sclerotinia sclerotiorum</i> strains from sclerotium samples in Quincy, Washington State, USA, and found that four single-sclerotium-isolation strains (PB4, PB273, PB615, and PB623) had almost identical genomes to the reference strain 1980 isolated in the west of Nebraska 40 years ago. The genome of strain PB4 sequenced by the next-generation sequencing (NGS) and Pacific Biosciences (PacBio) sequencing carried only 135 single nucleotide polymorphisms (SNPs) and 18 structural variations (SVs) compared with the genome of strain 1980 and 48 SNPs were distributed on Contig_20. Based on data generated by NGS, three other strains, PB273, PB615, and PB623, had 256, 275, and 262 SNPs, respectively, against strain 1980, which were much less than in strain PB4 (532 SNPs) and none of them occurred on Contig_20, suggesting much closer genomes to strain 1980 than to strain PB4. All other strains from America and China are rich in SNPs with a range of 34,391-77,618 when compared with strain 1980. We also found that there were 39-79 SNPs between strain PB4 and its sexual offspring, 53.1% of which also occurred on Contig_20. Our discoveries show that there are two types of genomes in <i>S. sclerotiorum</i>, one is very stable and the other tends to change constantly. Investigating the mechanism of such genome stability will enhance our understanding of heredity and variation.</p>","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40482851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Characterization of a QTL for Growth of <i>Fusarium circinatum</i> on Pine-Based Medium.","authors":"Benedicta S Swalarsk-Parry,&nbsp;Emma T Steenkamp,&nbsp;Stephanie van Wyk,&nbsp;Quentin C Santana,&nbsp;Magriet A van der Nest,&nbsp;Almuth Hammerbacher,&nbsp;Brenda D Wingfield,&nbsp;Lieschen De Vos","doi":"10.3390/jof8111214","DOIUrl":"https://doi.org/10.3390/jof8111214","url":null,"abstract":"<p><p><i>Fusarium circinatum</i> is an economically important pathogen of pine and resides in the <i>Fusarium fujikuroi</i> species complex. Here we investigated the molecular processes underlying growth in <i>F. circinatum</i> by exploring the association between growth and the nutritional environment provided by the pine host. For this purpose, we subjected a mapping population consisting of <i>F. circinatum</i> X <i>F. temperatum</i> hybrid progeny to an analysis of growth rate on a pine-tissue derived medium. These data, together with the available genetic linkage map for <i>F. circinatum</i>, were then used to identify Quantitative Trait Loci (QTLs) associated with growth. The single significant QTL identified was then characterized using the available genome sequences for the hybrid progeny's parental isolates. This revealed that the QTL localized to two non-homologous regions in the <i>F. circinatum</i> and <i>F. temperatum</i> genomes. For one of these, the <i>F. circinatum</i> parent contained a two-gene deletion relative to the <i>F. temperatum</i> parent. For the other region, the two parental isolates encoded different protein products. Analysis of repeats, G+C content, and repeat-induced point (RIP) mutations further suggested a retrotransposon origin for the two-gene deletion in <i>F. circinatum</i>. Nevertheless, subsequent genome and PCR-based analyses showed that both regions were similarly polymorphic within a collection of diverse <i>F. circinatum</i>. However, we observed no clear correlation between the respective polymorphism patterns and growth rate in culture. These findings support the notion that growth is a complex multilocus trait and raise the possibility that the identified QTL contains multiple small-effect QTLs, of which some might be dependent on the genetic backgrounds. This study improved our current knowledge of the genetic determinants of vegetative growth in <i>F. circinatum</i> and provided an important foundation for determining the genes and processes underpinning its ability to colonize its host environment.</p>","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40482853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial Distribution of Canine Sporotrichosis in Rio de Janeiro, Brazil (1998-2018) and Its Correlation with Socioeconomic Conditions.","authors":"Anna Barreto Fernandes Figueiredo,&nbsp;Mônica de Avelar Figueiredo Mafra Magalhães,&nbsp;Wagner de Souza Tassinari,&nbsp;Isabella Dib Ferreira Gremião,&nbsp;Luisa Helena Monteiro de Miranda,&nbsp;Rodrigo Caldas Menezes,&nbsp;Sandro Antonio Pereira","doi":"10.3390/jof8111207","DOIUrl":"https://doi.org/10.3390/jof8111207","url":null,"abstract":"<p><p>Canine sporotrichosis is a poorly described global disease and a spatial approach has not yet been used to assess the disease in dogs. Therefore, this study aimed to describe the occurrence of canine sporotrichosis in the state of Rio de Janeiro, Brazil, from 1998 to 2018 and its correlation with socioeconomic characteristics using exploratory spatial data analysis. A total of 295 cases of canine sporotrichosis were identified and 249 were georeferenced. There was a higher concentration of cases in the municipality of Rio de Janeiro, as well as along the border of the city and the adjacent municipalities in the great metropolitan area. The cases occurred in areas where most of the dwellings are houses. Moreover, no focus of disease density was found in the southern part of Rio de Janeiro city over the period studied, possibly due to better socioeconomic conditions. Areas with a high concentration of canine sporotrichosis cases coincided with regions that possessed a low proportion of households without paving, suggesting that the disease is not necessarily linked to extreme poverty. The mapping of areas with a greater density of cases is fundamental to formulate targeted and strategic plans in order to implement effective public health prevention and control measures.</p>","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9696400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40492992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Biofilm Formation by <i>Malassezia pachydermatis</i> Isolates and Its Susceptibility to Azole Antifungals.","authors":"Eva Čonková,&nbsp;Martina Proškovcová,&nbsp;Peter Váczi,&nbsp;Zuzana Malinovská","doi":"10.3390/jof8111209","DOIUrl":"https://doi.org/10.3390/jof8111209","url":null,"abstract":"<p><p>The yeast <i>Malassezia pachydermatis</i>, an opportunistic pathogen that inhabits the skin of various domestic and wild animals, is capable of producing a biofilm that plays an important role in antifungal resistance. The aim of this research study was to find the intensity of biofilm production by <i>M. pachydermatis</i> strains isolated from the ear canal of healthy dogs, and to determine the susceptibility of planktonic, adhered and biofilm-forming cells to three azole antifungals-itraco-nazole, voriconazole and posaconazole-that are most commonly used to treat <i>Malassezia</i> infections. Out of 52 isolates, 43 <i>M. pachydermatis</i> strains (82.7%) were biofilm producers with varying levels of intensity. For planktonic cells, the minimum inhibitory concentration (MIC) range was 0.125-2 µg/mL for itraconazole, 0.03-1 µg/mL for voriconazole and 0.03-0.25 µg/mL for posaconazole. Only two isolates (4.7%) were resistant to itraconazole, one strain (2.3%) to voriconazole and none to posaconazole. For adhered cells and the mature biofilm, the following MIC ranges were found: 0.25-16 µg/mL and 4-16 µg/mL for itraconazole, 0.125-8 µg/mL and 0.25-26 µg/mL for voriconazole, and 0.03-4 µg/mL and 0.25-16 µg/mL for posaconazole, respectively. The least resistance for adhered cells was observed for posaconazole (55.8%), followed by voriconazole (62.8%) and itraconazole (88.4%). The mature biofilm of <i>M. pachydermatis</i> showed 100% resistance to itraconazole, 95.3% to posaconazole and 83.7% to voriconazole. The results of this study show that higher concentrations of commonly used antifungal agents are needed to control infections caused by biofilm-forming strains of <i>M. pachydermatis</i>.</p>","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40492996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity Analysis of the Rice False Smut Pathogen <i>Ustilaginoidea virens</i> in Southwest China.","authors":"Rongtao Fu,&nbsp;Cheng Chen,&nbsp;Jian Wang,&nbsp;Yao Liu,&nbsp;Liyu Zhao,&nbsp;Daihua Lu","doi":"10.3390/jof8111204","DOIUrl":"https://doi.org/10.3390/jof8111204","url":null,"abstract":"Rice false smut caused by Ustilaginoidea virens is a destructive disease in rice cropping areas of the world. The present study is focused on the morphology, pathogenicity, mating-type loci distribution, and genetic characterization of different isolates of U. virens. A total of 221 strains of U. virens were collected from 13 rice-growing regions in southwest China. The morphological features of these strains exhibited high diversity, and the pathogenicity of the smut fungus showed significant differentiation. There was no correlation between pathogenicity and sporulation. Mating-type locus (MAT) analysis revealed that all 221 isolates comprised heterothallic and homothallic forms, wherein 204 (92.31%) and 17 (7.69%) isolates belonged to heterothallic and homothallic mating types, respectively. Among 204 strains of heterothallic mating types, 62 (28.05%) contained MAT1-1-1 idiomorphs, and 142 isolates (64.25%) had the MAT1-2-1 idiomorph. Interestingly, strains isolated from the same fungus ball had different mating types. The genetic structure of the isolates was analyzed using simple sequence repeats (SSRs) and single-nucleotide polymorphisms (SNPs). All isolates were clustered into five genetic groups. The values of Nei’s gene diversity (H) and Shannon’s information index (I) indicated that all strains as a group had higher genetic diversity than strains from a single geographical population. The pairwise population fixation index (FST) values also indicated significant genetic differentiation among all compared geographical populations. The analysis of molecular variation (AMOVA) indicated greater genetic variation within individual populations and less genetic variation among populations. The results showed that most of the strains were not clustered according to their geographical origin, showing the rich genetic diversity and the complex and diverse genetic background of U. virens in southwest China. These results should help to better understand the biological and genetic diversity of U. virens in southwest China and provide a theoretical basis for building effective management strategies.","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40492991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of <i>Dekkera bruxellensis</i> Concentration and Inoculation Time on Biochemical Changes and Cellulose Biosynthesis by <i>Komagataeibacter intermedius</i>.","authors":"Putu Virgina Partha Devanthi,&nbsp;Ferren Pratama,&nbsp;Katherine Kho,&nbsp;Mohammad J Taherzadeh,&nbsp;Solmaz Aslanzadeh","doi":"10.3390/jof8111206","DOIUrl":"https://doi.org/10.3390/jof8111206","url":null,"abstract":"<p><p>Bacterial Cellulose (BC) is a biopolymer with numerous applications. The growth of BC-producing bacteria, <i>Komagataeibacter intermedius</i>, could be stimulated by <i>Dekkera bruxellensis</i>, however, the effect on BC yield needs further investigation. This study investigates BC production and biochemical changes in the <i>K. intermedius-D. bruxellensis</i> co-culture system. <i>D. bruxellensis</i> was introduced at various concentrations (10³ and 10⁶ CFU/mL) and inoculation times (days 0 and 3). BC yield was ~24% lower when <i>D. bruxellensis</i> was added at 10³ CFU/mL compared to <i>K. intermedius</i> alone (0.63 ± 0.11 g/L). The lowest BC yield was observed when 10³ CFU/mL yeast was added on day 0, which could be compromised by higher gluconic acid production (10.08 g/L). In contrast, BC yields increased by ~88% when 10⁶ CFU/mL <i>D. bruxellensis</i> was added, regardless of inoculation time. High BC yield might correlate with faster sugar consumption or increased ethanol production when 10⁶ CFU/mL <i>D. bruxellensis</i> was added on day 0. These results suggest that cell concentration and inoculation time have crucial impacts on species interactions in the co-culture system and product yield.</p>","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9697449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40492990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Species and Records of <i>Dictyosporiaceae</i> from Freshwater Habitats in China and Thailand.","authors":"Hongwei Shen,&nbsp;Danfeng Bao,&nbsp;Dhanushka N Wanasinghe,&nbsp;Saranyaphat Boonmee,&nbsp;Jiankui Liu,&nbsp;Zonglong Luo","doi":"10.3390/jof8111200","DOIUrl":"https://doi.org/10.3390/jof8111200","url":null,"abstract":"<p><p>China and Thailand are rich in fungal diversity with abundant freshwater resources that are favorable for numerous fungal encounters. Resulting from this, the majority of the <i>Dictyosporiaceae</i> species reported were from these two countries. During the investigation on the diversity of lignicolous freshwater fungi in the Greater Mekong Subregion, eleven collections of cheirosporous species on submerged wood were collected from lentic and lotic habitats in China and Thailand. Phylogenetic analysis that combined nuclear small-subunit ribosomal RNA (SSU), internal transcribed spacer region (ITS), nuclear large subunit ribosomal RNA (LSU) and translation elongation factor 1α (<i>tef 1-α</i>) loci revealed six new species: <i>Dictyocheirospora chiangmaiensis</i>, <i>D. multiappendiculata</i>, <i>D. suae</i>, <i>Digitodesmium aquaticum</i>, <i>Vikalpa grandispora</i> and <i>V. sphaerica.</i> In addition, four known species were also identified and reported based on morphological and phylogenetic evidence. The detailed descriptions and illustrations of these taxa are provided with an updated phylogenetic tree of <i>Dictyosporiaceae</i>.</p>","PeriodicalId":520671,"journal":{"name":"Journal of fungi (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9694895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40722053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}