Applied immunohistochemistry & molecular morphology : AIMM最新文献

{"title":"Immunohistochemical expression of ERα, ERβ, and TFF1 in type I and II ovarian tumors.","authors":"Asmaa G Abdou,&nbsp;Hayam A Aiad,&nbsp;Nancy Y Asaad,&nbsp;Moshira M Abd El-Wahed,&nbsp;Marwa Serag El-Dien","doi":"10.1097/PAI.0b013e31829bebf6","DOIUrl":"https://doi.org/10.1097/PAI.0b013e31829bebf6","url":null,"abstract":"<p><p>Surface epithelial tumors of the ovary are no longer considered as a single disease but are divided into types I and II on the basis of their molecular features, cell of origin, and their behavior. A possible direct action of gonadal steroids on ovarian carcinogenesis has been suggested. The current information about the possible role of TFF1 in ovarian tumors,, together with its relationship to the estrogen receptor (ER) status, is insufficient. The aim of this study was to investigate ERα, ERβ, and TFF1 expression in type I and II ovarian tumors and their correlation with clinicopathologic parameters of each type. The present study was carried out on 97 ovarian tumors [20 benign, 15 borderline, and 62 malignant (36 type I and 26 type II tumors)]. ERα expression was significantly in favor of type II tumors (P=0.04), whereas high TFF1 expression was significantly in favor of type I tumors (P=0.02). ERα and ERβ showed a significant positive correlation in benign cases (P=0.004) and in type I tumors (P=0.006), but not in type II tumors. In type I tumors, the expression of ERα was correlated with serous carcinoma (P=0.002) and bilaterality (P=0.05), whereas TFF1 was correlated with mucinous carcinoma (P=0.02), unilaterality (P=0.04), early FIGO staging (P=0.01), and a low mitotic count (P=0.03). A high ERβ:ERα H score ratio was associated with advanced FIGO staging in both type I (P=0.05) and type II tumors (P=0.009). The difference in the expression of ERα and TFF1 between type I and II tumors may be indicative of the difference in their origin and molecular pathway. The ERβ:ERα ratio is more important in determining the net result of ER effects than the evaluation of each receptor separately, and the high ratio may promote progression to advanced stage in type I and II ovarian tumors. High TFF1 expression in ovarian mucinous carcinoma may indicate that their mucinous differentiation is toward an intestinal type rather than an endocervical type. TFF1 expression in ovarian tumors seems to occur independent of the status of the ER.</p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"421-32"},"PeriodicalIF":1.6,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0b013e31829bebf6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40267375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value of podoplanin as an immunohistochemical marker in tumor diagnosis: a review and update.","authors":"Nelson G Ordóñez","doi":"10.1097/PAI.0b013e31828a83c5","DOIUrl":"https://doi.org/10.1097/PAI.0b013e31828a83c5","url":null,"abstract":"<p><p>Podoplanin is a type I integral membrane glycoprotein that, because it is expressed in lymphatic endothelium, but not in vascular blood vessel endothelial cells, is commonly used in the identification of lymphatic endothelial differentiation in vascular endothelial neoplasms and lymphatic invasion by tumor. Because podoplanin is also expressed in mesothelial cells and fetal gonocytes, it has proved to be a useful marker for assisting in the differential diagnosis of mesotheliomas and germ cell tumors, particularly seminomas/dysgerminomas. Podoplanin expression has also been reported in a wide variety of other neoplasms, including hemangioblastomas, meningiomas, cartilaginous tumors, and follicular dendritic cell neoplasms. This article reviews the information that is currently available on the application of podoplanin immunostaining in diagnostic pathology. </p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"331-47"},"PeriodicalIF":1.6,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0b013e31828a83c5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40228331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated objective determination of percentage of malignant nuclei for mutation testing.","authors":"Hollis Viray,&nbsp;Madeline Coulter,&nbsp;Kevin Li,&nbsp;Kristin Lane,&nbsp;Aruna Madan,&nbsp;Kisha Mitchell,&nbsp;Kurt Schalper,&nbsp;Clifford Hoyt,&nbsp;David L Rimm","doi":"10.1097/PAI.0b013e318299a1f6","DOIUrl":"https://doi.org/10.1097/PAI.0b013e318299a1f6","url":null,"abstract":"<p><p>Detection of DNA mutations in tumor tissue can be a critical companion diagnostic test before prescription of a targeted therapy. Each method for detection of these mutations is associated with an analytic sensitivity that is a function of the percentage of tumor cells present in the specimen. Currently, tumor cell percentage is visually estimated resulting in an ordinal and highly variant result for a biologically continuous variable. We proposed that this aspect of DNA mutation testing could be standardized by developing a computer algorithm capable of accurately determining the percentage of malignant nuclei in an image of a hematoxylin and eosin-stained tissue. Using inForm software, we developed an algorithm, to calculate the percentage of malignant cells in histologic specimens of colon adenocarcinoma. A criterion standard was established by manually counting malignant and benign nuclei. Three pathologists also estimated the percentage of malignant nuclei in each image. Algorithm #9 had a median deviation from the criterion standard of 5.4% on the training set and 6.2% on the validation set. Compared with pathologist estimation, Algorithm #9 showed a similar ability to determine percentage of malignant nuclei. This method represents a potential future tool to assist in determining the percent of malignant nuclei present in a tissue section. Further validation of this algorithm or an improved algorithm may have value to more accurately assess percentage of malignant cells for companion diagnostic mutation testing. </p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"363-71"},"PeriodicalIF":1.6,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0b013e318299a1f6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40268835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characteristics of breast cancer subtypes: implications for treatment guidelines and individualized treatment strategies in China.","authors":"Shan Zheng,&nbsp;Qing-Kun Song,&nbsp;Yu Ren,&nbsp;Wei-Liang Feng,&nbsp;Ya-Nan Kong,&nbsp;Rong Huang,&nbsp;Feng Xu,&nbsp;Jing Li,&nbsp;Bao-Ning Zhang,&nbsp;Jin-Hu Fan,&nbsp;Jian-Jun He,&nbsp;You-Lin Qiao","doi":"10.1097/PAI.0b013e3182a3c52c","DOIUrl":"https://doi.org/10.1097/PAI.0b013e3182a3c52c","url":null,"abstract":"<p><p>This study aimed at investigating the characteristics of invasive breast cancer among molecular subtypes. Patients with invasive breast cancer, with complete information on the expressions of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2 (HER2), were recruited. χ tests and an unconditional logistic regression model were used for statistical analysis. The percentages of luminal A, luminal B, HER2/neu, and triple-negative subtypes were 54.2% (1639/3021), 14.0% (422/3021), 8.9% (269/3021), and 22.9% (691/3021), respectively. Differences among molecular subtypes (P<0.05) in tumor size, stage, pathologic type, and lymph node status were observed. The HER2/neu, luminal B, and triple-negative subtypes were more aggressive compared with the luminal A subtype in tumor stage, lymph node status, or pathologic type (P<0.05), when the findings were adjusted for age. Molecular subtypes were distributed differently between both age groups and regional groups on the basis of the socioeconomic status (P<0.05). In conclusion, luminal A and triple-negative subtypes were the 2 main subtypes of invasive breast cancer in China. The variations of molecular subtypes in pathology, age, and regional distribution may give some suggestions for updating treatment guidelines and individualized treatment strategies in China. </p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"383-9"},"PeriodicalIF":1.6,"publicationDate":"2014-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0b013e3182a3c52c","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40267380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sorting Nexin 2 (SNX2): a potential marker of active thyrocytes in normal and hyperfunctioning thyroid disorders.","authors":"Maki Kanzawa,&nbsp;Shigeo Hara,&nbsp;Shuho Semba,&nbsp;Hiroshi Yokozaki,&nbsp;Mitsuyoshi Hirokawa,&nbsp;Tomoo Itoh","doi":"10.1097/PAI.0b013e31828badd3","DOIUrl":"https://doi.org/10.1097/PAI.0b013e31828badd3","url":null,"abstract":"<p><p>Sorting nexins (SNXs) are a large, diverse group of cytoplasmic and membrane-associated proteins that function in a variety of cellular processes, including endocytosis, protein trafficking, and the retrieval of transmembrane proteins. In this study, we demonstrated that SNX2 is expressed in columnar and active thyroid follicular cells but not in flattened inactive thyrocytes. Morphometric analysis revealed a significant correlation between SNX2 positivity and columnar cell morphology. Immunohistochemical staining of serial sections of the thyroid tissue indicated that SNX2 localization was similar to sortilin, a protein expressed by active thyrocytes. Expression of SNX2 in thyrocytes is particularly marked and extensive in most hyperstimulated thyroid disorders, including Graves disease (diffusely SNX2 positive in 73.3% patients) and functioning nodules (93.8% patients). SNX2 immunolocalization in hyperstimulated follicular epithelial cells was specific among the SNXs family members examined. These results support the utility of SNX2 as a novel marker of active thyrocytes and reflect the endosomal trafficking activity in these cells. </p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"302-7"},"PeriodicalIF":1.6,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0b013e31828badd3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40228334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-slide, quadruple immunofluorescence labeling of routinely processed paraffin sections.","authors":"Serena Buscone,&nbsp;Maria C Argentieri,&nbsp;Daniela Pilla,&nbsp;Giorgio Cattoretti","doi":"10.1097/PAI.0b013e31829928e7","DOIUrl":"https://doi.org/10.1097/PAI.0b013e31829928e7","url":null,"abstract":"<p><p>Whole-slide images (WSI) have acquired a stable place in diagnostic histopathology and immunohistochemistry. Immunofluorescence (IF) techniques hold a limited and selective role in diagnostics (eg, renal and cutaneous pathology) and so far remain excluded from the digital pathology evolution, with notable exceptions, such as quantitative immunopathology. We explored the ability of a commercial fluorescent slide scanner to provide 4-color IF WSI from routinely processed tissues. With minor modifications and a careful match between filters and fluorochromes, we show that 4-color IF WSI can be obtained from routine material with negligible autofluorescence, good sensitivity, and diagnostic power. </p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"e1-7"},"PeriodicalIF":1.6,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0b013e31829928e7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40267376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association of urocortin expression with clinicopathologic parameters of gastric adenocarcinomas.","authors":"Ming-Fang Cheng,&nbsp;Wen-Chiuan Tsai,&nbsp;Lin Yu-Chieh,&nbsp;Kan-Tai Hsia,&nbsp;Jong-Shiaw Jin","doi":"10.1097/PAI.0b013e31828adbfa","DOIUrl":"https://doi.org/10.1097/PAI.0b013e31828adbfa","url":null,"abstract":"<p><p>Gastric adenocarcinoma is a lethal disease with high incidence in Chinese people. The purpose of this study was to evaluate the expression of urocortin (UCN) in normal gastric mucosa and gastric adenocarcinomas. Immunohistochemical analysis of UCN was performed in 112 surgical specimens (21 normal gastric mucosa specimens and 91 gastric adenocarcinoma specimens varying in histologic grade and pathologic stage). Immunostain intensity was scored on a scale ranging from 0 (absence of staining) to 3 (strong staining). The percentage of UCN stained cells was scored on a scale ranging from 0 (<5%) to 4 (75% to 100%). The UCN immunoscore (ranging from 0 to 12) was the product of the above 2 scores. The UCN immunoscore was high in all 9 normal gastric mucosa specimens, significantly lower in poorly differentiated gastric adenocarcinoma than in well and moderately differentiated tumors (P=0.018), and significantly lower in more advanced pathologic stages of gastric adenocarcinomas than in the early stages of these tumors. Moreover, UCN expression was higher in gastric adenocarcinomas with neuroendocrine differentiation than in mucinous adenocarcinomas and signet-ring cell carcinomas. In conclusion, UCN is expressed in most non-neoplastic gastric glandular epithelia. However, UCN expression inversely correlates with higher tumor grade and advanced TNM stage in gastric adenocarcinomas. </p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"288-94"},"PeriodicalIF":1.6,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PAI.0b013e31828adbfa","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40228330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance evaluation comparison of 3 commercially available PCR-based KRAS mutation testing platforms.","authors":"Julia A Adams,&nbsp;Kristin M Post,&nbsp;Sarah A Bilbo,&nbsp;Xiaoyan Wang,&nbsp;Joyashree D Sen,&nbsp;Anita J Cornwell,&nbsp;Amanda J Malek,&nbsp;Liang Cheng","doi":"10.1097/PDM.0b013e3182a127f9","DOIUrl":"https://doi.org/10.1097/PDM.0b013e3182a127f9","url":null,"abstract":"<p><p>The identification of KRAS mutations in patients with certain types of cancer, including colonic adenocarcinoma and non-small cell lung carcinoma, has become increasingly important as these patients are contraindicated from receiving epidermal growth factor receptor-targeted therapies. Several polymerase chain reaction (PCR)-based tests are commercially available for KRAS mutation testing including Applied Biosystems KRAS Mutation Analysis on the ABI3130xl, Qiagen therascreen KRAS RGQ PCR on the Rotor-Gene Q MDx, and Qiagen KRAS Pyro on the PyroMark Q24; however, these tests have not been compared side by side. The purpose of this study was to evaluate the performance characteristics and workflow for 3 PCR-based methods of detecting KRAS mutation status. We evaluated the performance characteristics and workflow for 3 commercially available KRAS mutation detection platforms. All of the 188 samples run were successful, with 29% being positive for the KRAS mutation. Of the positive tests, Applied Biosystems detected 84% of the positive cases, whereas Qiagen therascreen RGQ and Qiagen KRAS Pyro detected 100% of the positive cases. In cases of discrepancy between Applied Biosystems and therascreen RGQ, Pyro agreed with therascreen RGQ 95% of the time. Qiagen therascreen RGQ and Pyro were comparable in terms of sensitivity, specificity, positive predictive value, negative predictive value, and accuracy, with all values being 100%. All 3 techniques accurately identified the appropriate mutation in the known control specimens. In summary, all 3 tests are relatively comparable for detecting the KRAS mutation, with Applied Biosystems having a slightly lower sensitivity, negative predictive value, and accuracy than therascreen RGQ and Pyro. </p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"231-5"},"PeriodicalIF":1.6,"publicationDate":"2014-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PDM.0b013e3182a127f9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40297116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of the Rac1/RhoA pathway with ezrin expression in osteosarcoma.","authors":"Caterina Chiappetta,&nbsp;Martina Leopizzi,&nbsp;Fabiana Censi,&nbsp;Chiara Puggioni,&nbsp;Vincenzo Petrozza,&nbsp;Carlo D Rocca,&nbsp;Claudio Di Cristofano","doi":"10.1097/PDM.0000000000000033","DOIUrl":"https://doi.org/10.1097/PDM.0000000000000033","url":null,"abstract":"<p><p>Osteosarcoma is the most common malignant tumor of the bone. The major cause of death in osteosarcoma is the increase in metastatic potential, and the ezrin expression has been correlated with the metastasis development. Ezrin interacts with RhoGDI by dissociating it from RhoGTPases, which allow GTPases to load with GTP, activate RhoA to increase cell migration, and invasion. RhoGTPases have been found to contribute to pathologic processes including cancer cell migration, invasion, and metastasis and overexpression of either the GTPase itself or some elements of Rho signaling that have been detected in many human tumors, including Rac1 and RhoA. We have analyzed Rac1 and RhoA expression in the osteosarcoma tissues to understand the role of the ezrin-Rho family pathway in osteosarcoma metastatic progression. Moreover, we have blocked the ezrin expression using siRNA assay to investigate a possible correlation with RAC1 and RHOA expression in the osteosarcoma cell lines. Our immunohistochemical data showed that many osteosarcomas presented cytoplasmatic positivity for both Rac1 and RhoA and cases, both ezrin positive than ezrin negative, revealed the protein expression of Rac1 and RhoA. The results obtained by ezrin siRNA transfection showed that ezrin expression in the osteosarcoma cell lines might modulate, mainly, the Rac1 expression. It is possible that the mechanism of cell motility mediated by Rac1 and RhoA is maintained in osteosarcomas, and since the expression of ezrin, Rac1 and RhoA do not correlate with metastatic progression in osteosarcoma. However, osteosarcomas without metastasis displayed a positivity for Rac1 and RhoA expression compared with metastatic osteosarcomas and this could be a protective factor. </p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"162-70"},"PeriodicalIF":1.6,"publicationDate":"2014-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PDM.0000000000000033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40297948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study of alveolar rhabdomyosarcoma copy number alterations by single nucleotide polymorphism analysis.","authors":"Miriam Lynn,&nbsp;Naisha Shah,&nbsp;Judith Conroy,&nbsp;Sean Ennis,&nbsp;Thomas Morris,&nbsp;David Betts,&nbsp;Maureen O'Sullivan","doi":"10.1097/PDM.0000000000000030","DOIUrl":"https://doi.org/10.1097/PDM.0000000000000030","url":null,"abstract":"<p><p>Rhabdomyosarcoma, the most common pediatric soft tissue malignancy arises in 2 major histologic forms: embryonal and alveolar. Classically, the alveolar subtype is characterized by a chromosomal translocation t(2;13)(q35;q14) or t(1;13)(p36;q14) fusing the PAX3 or PAX7 gene, respectively, to the FOXO1 gene, although fusion-negative cases of alveolar rhabdomyosarcoma (ARMS) occur; these share considerably more with the genomic profiles and biological behavior of embryonal rhabdomyosarcoma than with fusion-positive ARMS. The current understanding of any additional genetic aberrations in fusion-positive ARMS is limited. In this study, we evaluated tumor-specific copy number alterations in a cohort of fusion-positive ARMSs using high-resolution technology. The results presented here include previously described changes as well as completely novel findings of copy number alterations in BCR and DICER. The study furthermore highlights associations between fusion type and genotype, as well as outcomes and genotype. Rearrangement of PAX7 is strongly associated with copy number alteration of Glypican 5 (GPC5) and moderately with amplification of IGF1R. There is a moderate association between death from/relapse of disease and, on the one hand, amplification of 12q13.3 (DDIT3; Gli1), and on the other hand, copy number alteration of Wnt6 or LRP1B. Gains of both LRP1B and Gli1 in turn are strongly associated with MycN amplification. </p>","PeriodicalId":520562,"journal":{"name":"Applied immunohistochemistry & molecular morphology : AIMM","volume":" ","pages":"213-21"},"PeriodicalIF":1.6,"publicationDate":"2014-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1097/PDM.0000000000000030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40297115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}