Pediatric discovery最新文献

{"title":"Identification of Prognostic Biomarkers in Gene Expression Profile of Neuroblastoma Via Machine Learning.","authors":"Shuxin Tang, Jinhua Fan, Yupeng Cun","doi":"10.1002/pdi3.70009","DOIUrl":"10.1002/pdi3.70009","url":null,"abstract":"<p><p>Neuroblastoma (NB) is a common pediatric solid malignancy characterized by heterogeneous clinical outcomes. The identification of predictive and interpretable prognostic biomarkers is critical for advancing precision medicine in NB. We proposed an integrative network-based machine learning method for biomarker discovery, which employed a network smoothed <i>t</i>-statistic support vector machine to select prognostic related biomarkers, and then we performed network analysis on these biomarkers to find hub genes. Later, we conducted a comprehensive analysis to integrate bulk and single-cell RNA sequencing data to character the tumor microenvironment of prognostic state and correlated them to the discovered hub genes. This analysis identified 528 prognostic biomarkers associated with NB. Network-based analysis further refined this set to 11 hub prognostic biomarkers for NB: <i>AURKA</i>, <i>BLM</i>, <i>BRCA1</i>, <i>BRCA2</i>, <i>CCNA2</i>, <i>CHEK1</i>, <i>E2F1</i>, <i>MAD2L1</i>, <i>PLK1</i>, <i>RAD51</i>, and <i>RFC3</i>. Among these genes, high <i>RFC3</i> expression was significantly associated with poor prognosis, highlighting its potential as a novel prognostic biomarker in NB. Additionally, our findings revealed that these biomarkers are correlated to chemotherapy drugs, such as vincristine and cyclophosphamide. Furthermore, drug sensitivity analyses identified several candidate drugs, such as dactinomycin, bortezomib, docetaxel, and sepantronium bromide, that may hold therapeutic potential for NB treatment. This study offers novel insights to underlying NB prognosis and therapeutic targets and provides a foundation for developing personalized treatment strategies to improve clinical outcomes.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 2","pages":"e70009"},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Authors' Reply to PDI-2024-0087: Letter to the Editor for \"Metronomic Chemotherapy in Pediatric Neuroblastoma\".","authors":"Chao Yang","doi":"10.1002/pdi3.70008","DOIUrl":"10.1002/pdi3.70008","url":null,"abstract":"","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 2","pages":"e70008"},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk Factors and Clinical Features of Deterioration in 739 COVID-19 Affected Children Aged Under 14 Years in Zhuhai, China: A Multicenter, Retrospective Study.","authors":"Ting Liu, Zhaojun Pan, Cheng Zhang, Fanlin Huang, Jundong Ding, Wenxiu Song, Yongwu Xie, Guang Lin","doi":"10.1002/pdi3.70001","DOIUrl":"10.1002/pdi3.70001","url":null,"abstract":"<p><p>To retrospectively analyze the clinical characteristics of children with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, this study involved 739 children with SARS-CoV-2 infection who were admitted to multiple medical institutions in Zhuhai City from December 15, 2022 to January 24, 2023. Epidemiological and clinical data were collected and analyzed using statistical methods to understand the disease characteristics. The onset and progression of SARS-CoV-2 infection in children were significantly associated with age distribution, basic illness, vaccination status, exposure history, and family clustering. The most common clinical symptoms were fever (91.7%) and cough (81.6%). Laboratory findings indicated elevated neutrophil count, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, prothrombin time, and procalcitonin, creatine kinase, D-dimer, and IL-6 levels, along with decreased lymphocyte count, platelet count, and lymphocyte-to-monocyte ratio. Lung computed tomography (CT) showed imaging changes strongly linked to severe infection. After receiving anti-inflammatory, symptomatic, supportive, and antipathogen therapies, 74% of the children displayed clinical symptomatic relief, 26% of the children were cured and discharged, and there were no fatalities. In Zhuhai, children infected with SARS-CoV-2 commonly exhibit family-based transmission, with fever and cough as the predominant symptoms. Factors, such as young age, basic illness, specific laboratory markers, and patchy exudative changes on lung CT scans, served as critical indicators of severe infection. Early detection and monitoring of these factors, along with timely vaccination against novel coronavirus, can mitigate disease severity and prevent progression.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 2","pages":"e70001"},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effects of <i>Phyllanthus urinaria L</i> on Cisplatin-Induced Acute Kidney Injury: Anti-Inflammatory, Anti-Apoptotic, and Anti-Oxidative Action.","authors":"Qitong Guo, Ping Li, Meiling Chen, Yihang Yu, Lianju Shen, Chunlan Long, Xing Liu, Guanghui Wei, Deying Zhang","doi":"10.1002/pdi3.70000","DOIUrl":"10.1002/pdi3.70000","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is a prevalent and serious clinical challenge with limited specific treatments. <i>Phyllanthus urinaria L</i> (<i>PU</i>), a well-regarded herbal medicine, possesses noted anti-inflammatory, antiviral, and antitumor properties. Yet, research into its renal protective effects, particularly for cisplatin-induced AKI, is markedly limited. Our study explores the therapeutic potential of <i>PU</i> against AKI triggered by cisplatin administration. Utilizing an AKI model induced by a substantial dose of cisplatin, we assessed renal function through histological examination (hematoxylin and eosin staining) and biochemical markers, including blood urea nitrogen (BUN) and serum creatinine (Scr). Our results demonstrated that <i>PU</i> may mitigate cisplatin's structural damage to renal tissue. Post-treatment with <i>PU</i>, a significant reduction in pro-inflammatory cytokines interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) was observed through quantitative polymerase chain reaction (PCR) analysis. Additionally, the expressions of apoptotic and anti-apoptotic proteins B cell leukemia/lymphoma two (Bcl-2), Bcl2-Associated X protein (Bax), and cleaved-caspase3 evaluated via Western blot and immunofluorescence indicated that <i>PU</i> could inhibit apoptosis in response to cisplatin injury. Oxidative stress markers, such as malondialdehyde (MDA) and superoxide dismutase (SOD), showed decreased MDA levels and elevated SOD activity following <i>PU</i> treatment, suggesting a reduction in oxidative damage. Furthermore, cisplatin-induced downregulation of <i>NRF2</i> and <i>NQO1</i> was countered by <i>PU</i>, highlighting its restorative effect on antioxidant pathways as confirmed by Western blot and immunofluorescence. Collectively, our findings endorse <i>PU</i>'s protective capability against cisplatin-induced AKI, endorsing its therapeutic potential to counteract cisplatin's nephrotoxicity.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 4","pages":"e70000"},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12753014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Review of the Impact of Maternal Stress on Fetal Development.","authors":"Divya Tadanki, Pranitha S Kaza, Elliana Meisinger, Ariana Syed, Asha Johnson, Garen Bainbridge, Michelle Cho, Chikaima Anigbogu, Gargi Gupta","doi":"10.1002/pdi3.70004","DOIUrl":"10.1002/pdi3.70004","url":null,"abstract":"<p><p>Maternal stress impacts millions of pregnant women across the globe each year and refers to mental or psychological stress that occurs during the prenatal period. Although it remains known that maternal stress can negatively impact maternal health, the downstream effects of stress on fetal and infant development in the long-term are unclear. This leaves a gap in understanding epigenetic factors that impact growth and development, highlighting the need to address potential developmental delays in early childhood. Review of the literature indicates debate over the effect of maternal stress on cognitive development and whether maternal stress is a mediator or causal factor in fetal development. Although evidence suggests that maternal stress disrupts the functional connectivity of the amygdala, information on the sub-regions of the brain impacted by prenatal stress exposure remains unknown. Studies focusing on environmental disasters and war have also indicated adverse impacts on fetal development, additionally emphasizing a greater need for representation in future research. Timing of fetal exposure to stressors also impacts different domains of development. The Georgia OASIS platform was utilized to investigate variations in reported pregnancies, reported fetal mortality rate, and Perinatal Periods of Risk (PPOR) during the years 2013-2023. Increases in fetal mortality were anticipated to be associated with decreases in the rates of maternal, newborn, and infant care. Although evidence exists to support this hypothesis, causation cannot be established; however, the poor state of maternal healthcare in Georgia warrants further investigation of the barriers to healthcare to improve fetal and infant health outcomes statewide.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 3","pages":"e70004"},"PeriodicalIF":0.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Progress in Treating Airway Mucus Hypersecretion by Targeting the Epidermal Growth Factor Receptor Signaling Pathway.","authors":"Yuanyuan Zhang, Fuping Yang, Chunyu Yan, Yu Deng, Enmei Liu","doi":"10.1002/pdi3.70007","DOIUrl":"10.1002/pdi3.70007","url":null,"abstract":"<p><p>Asthma, chronic pulmonary obstructive disease (COPD), cystic fibrosis, and acute respiratory infections are severe respiratory conditions that significantly contribute to global morbidity and mortality. Airway mucus hypersecretion is an important common pathophysiological and clinical manifestation of these diseases and is closely associated with adverse clinical outcomes. At present, the management of airway mucus hypersecretion lacks a viable clinical intervention. However, the revelation that the EGFR signaling pathway acts as a convergent intracellular pathway for many inflammatory mediators indicates that it exhibits the greatest potential for inhibiting excessive mucus production. This review provides a comprehensive overview of the role of EGFR in airway mucus hypersecretion and the therapeutic progress of targeting it, intending to improve clinicians' understanding of the mechanism and offer insights and recommendations for developing novel pharmaceutical interventions to regulate mucus secretion.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"4 1","pages":"e70007"},"PeriodicalIF":0.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13097361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147794743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Double Outlet Left Ventricle in Pediatrics Using Transthoracic Echocardiography and Computed Tomographic Angiography.","authors":"Xu Zhu, Guangyi Zhong, Xiaojuan Ji, Xue Xiang, Min Zheng, Huina Yan, Rui Li","doi":"10.1002/pdi3.70005","DOIUrl":"10.1002/pdi3.70005","url":null,"abstract":"<p><p>Double outlet left ventricle (DOLV) is a rare congenital cardiac anomaly in which both great arteries originate entirely or predominantly from the morphologic left ventricle. The aim of this study is to explore its clinical presentations and compare the diagnostic accuracy of transthoracic echocardiography (TTE) with computed tomographic angiography (CTA) on DOLV. We took TTE as a first-line examination modality, considering CTA and surgical results as the gold standard. Ten suspected patients with DOLV were identified at the Children's Hospital of Chongqing Medical University from March 2009 to November 2023. The clinical presentations, TTE, CTA, and follow-up data of the children were analyzed retrospectively. All 10 cases (100%) underwent TTE examination, and 7 patients (70%) received CTA examination. Six patients (60%) ultimately underwent surgery and were confirmed to have DOLV. DOLV were initially diagnosed using TTE with a diagnostic accuracy rate of 85.71% after comparisons with CTA and surgery results. Ventricular septal defect (100%) and atrial septal defect (80%) were the common associated abnormality. The clinical manifestation of DOLV was atypical, and TTE has a relatively high diagnostic accuracy for DOLV in pediatrics, making it a very valuable tool for early detection.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 4","pages":"e70005"},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12753015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translating OMWaNA Trial Findings and Exploring Wider Implications for KMC in Neonatal Care.","authors":"Aditya Hemendra Bhatt, Somashekhar Marutirao Nimbalkar, Dipen Vasudev Patel, Reshma Kushal Pujara","doi":"10.1002/pdi3.70006","DOIUrl":"10.1002/pdi3.70006","url":null,"abstract":"","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 2","pages":"e70006"},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Impact of Screen Time on the Motor Development of Children: A Systematic Review.","authors":"Danyal Bakht, Faiza Yousaf, Zainab Alvi, Muhammad Khan Buhadur Ali, Mirza Muhammad Hadeed Khawar, Luqman Munir, Syed Faqeer Hussain Bokhari, Muhammad Shoaib Qureshi, Mehnahil Raza, Ali Akram Qureshi","doi":"10.1002/pdi3.70002","DOIUrl":"10.1002/pdi3.70002","url":null,"abstract":"<p><p>Screen time, defined as the amount of time spent engaging with electronic screens, has become inevitable in modern life. The rise in screen time among children under 5 years old has raised concerns about its association with motor development including gross and fine motor skills. Conflicting evidence on the association of screen time requires more investigation and the planning of targeted interventions. This systematic review aims to explore the relationship between screen time and motor development in children aged 0-7 years, considering various influencing factors like screen type, exposure duration, and context. Following preferred reporting items for systematic reviews and meta-analyses (PRISMA) and assessing the methodological quality of systematic reviews (AMSTAR) guidelines, a literature search was conducted in May 2024 in databases including PubMed, Cochrane Central Register of Controlled Trials (CENTRAL), and ScienceDirect. Eligible studies were observational or experimental, involved children aged 0-7 years, and assessed the outcomes between screen time and motor development. The quality of the included studies was assessed through Joanna Briggs Institute's (JBI) critical appraisal checklist. Out of 1490 records initially identified, 24 studies met the inclusion criteria for this review. Among these, 17 studies reported a significant negative correlation between screen time and motor development in children, while 5 studies found no statistically significant association. Two studies presented mixed findings, indicating both negative and positive associations between screen time and motor development. Excessive screen time in early childhood is mainly linked to negative effects on motor development. The association varies with screen content and environment, highlighting the need for balanced screen time and early interventions.</p>","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 2","pages":"e70002"},"PeriodicalIF":0.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor for \"Metronomic Chemotherapy in Pediatric Neuroblastoma\".","authors":"Ana Ivanovski, Dimitrije Nikolic, Ana Cirovic, Aleksandar Cirovic","doi":"10.1002/pdi3.70003","DOIUrl":"10.1002/pdi3.70003","url":null,"abstract":"","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 2","pages":"e70003"},"PeriodicalIF":0.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}