Pediatric discovery最新文献_第10页

Continuous positive airway pressure in delivery room in extremely preterm infants: A single-center retrospective study in China. 中国极早产儿产房持续气道正压通气的单中心回顾性研究

Pediatric discovery Pub Date : 2024-10-25 eCollection Date: 2025-06-01 DOI: 10.1002/pdi3.2507

Xiaoting Zhang, Long Chen, Xiaoyun Zhong, Jiangfeng Ou, Yuan Shi

{"title":"Continuous positive airway pressure in delivery room in extremely preterm infants: A single-center retrospective study in China.","authors":"Xiaoting Zhang, Long Chen, Xiaoyun Zhong, Jiangfeng Ou, Yuan Shi","doi":"10.1002/pdi3.2507","DOIUrl":"10.1002/pdi3.2507","url":null,"abstract":"To assess the beneficial effects of delivery room continuous positive airway pressure (DRCPAP) in extremely preterm infants, a single-center retrospective study was performed at the Women and Children's Hospital of Chongqing Medical University in China. Infants born between January 2016 and December 2018 were regarded as the control group, and those born between January 2019 and August 2022 were considered as the observation group (DRCPAP group). The primary outcome was tracheal intubation within 72 h after birth. Six hundred and seven patients were included in the study (control: 232; DRCPAP: 375). Compared with the control group, DRCPAP reduced the intubation rate (56.8% vs. 62.9%, OR 0.57, 95% CI 0.34-0.96, p = 0.035), including <28 weeks gestational age (GA) subgroup (61.5% vs. 84.7%, OR 0.12, 95% CI 0.02-0.78, p = 0.027). One-to-one propensity score matching (195:195) was used to match the baseline characteristics of patients in DRCPAP and control group. After matching, no significant differences were observed in intubation rate within 72 h between the two groups (20.5% [40 of 195] vs. 22.1% [43 of 195]; p = 0.711). Whether DRCPAP can reduce intubation rate within 72 h requires further investigation.","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 2","pages":"e2507"},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waardenburg-Shah syndrome: A case of neonatal palliative care. Waardenburg-Shah综合征：新生儿姑息治疗一例。

Pediatric discovery Pub Date : 2024-09-24 eCollection Date: 2024-12-01 DOI: 10.1002/pdi3.2506

Michael Colpani, Maria Del Carmen Rodriguez Perez, Bruno Angelo Drera, Francesco Maria Risso

引用次数: 0

Perioperative hemodynamic parameters monitored by three noninvasive technologies in children with congenital heart disease: A prospective study. 三种无创技术监测先天性心脏病患儿围手术期血流动力学参数：一项前瞻性研究

Pediatric discovery Pub Date : 2024-09-12 eCollection Date: 2025-06-01 DOI: 10.1002/pdi3.2505

Xiaoyu Xiong, Feng Xu, Wei Qiu, Shaojun Li, Chengjun Liu

{"title":"Perioperative hemodynamic parameters monitored by three noninvasive technologies in children with congenital heart disease: A prospective study.","authors":"Xiaoyu Xiong, Feng Xu, Wei Qiu, Shaojun Li, Chengjun Liu","doi":"10.1002/pdi3.2505","DOIUrl":"10.1002/pdi3.2505","url":null,"abstract":"This study aims to compare the efficiencies of three noninvasive technologies in monitoring the perioperative hemodynamics of children with congenital heart disease (CHD) including ventricular septal defects with or without atrial septal defects. Three noninvasive technologies included transthoracic echocardiography (TTE), electrical cardiometry (EC), and vasoactive inotropic score (VIS). Parameters included left ventricular ejection fraction (LVEF) and cardiac index (cardiac index monitored by ultrasound, uCI) in TTE, cardiac index (cardiac index monitored by electrical cardiometry, eCI) and systemic vascular resistance index (SVRI) in EC, and VIS. Seventy-four children were eligible. Three types of adverse events (AEs) related to disease activity and prognosis were observed, including cardio-pulmonary resuscitation in five cases (5/74, 6.76%), hypoxic-ischemic brain damage in four cases (4/74, 5.41%) and hemopurification in four cases (4/74, 5.41%). Except for LVEF, eight parameters (VISmax [maximum VIS], VISmea [mean VIS], uCImea [mean uCI], uCImin [minimum uCI], eCImea [mean eCI], eCImin [minimum eCI], SVRImea [mean SVRI], and SVRImin [minimum SVRI]) showed predictive value for any AE (p < 0.05). VISmea, uCImea, and eCImea demonstrated the highest accuracy and linear associations (AUROC > 0.9, p = 0.00). Linear associations also existed between the three groups of parameters and the duration of mechanical ventilation (MV) and the length of stay (LOS) in the intensive care unit (ICU). The duration of MV and the LOS in the ICU increased as VISmea rose, or uCImea and eCImea fell (p < 0.05). LVEF in TTE could not predict any AE (p > 0.05) and not fully reflect the cardiovascular function. Therefore, most parameters obtained in TTE, EC, and VIS can reflect the perioperative hemodynamics of children with CHD, with VISmea, uCImea, and eCImea being most accurate.","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 2","pages":"e2505"},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144645135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiomyocyte proliferation and regeneration in congenital heart disease. 先天性心脏病的心肌细胞增殖与再生

Pediatric discovery Pub Date : 2024-09-01 Epub Date: 2024-08-12 DOI: 10.1002/pdi3.2501

Jialiang Liang, Xingyu He, Yigang Wang

{"title":"Cardiomyocyte proliferation and regeneration in congenital heart disease.","authors":"Jialiang Liang, Xingyu He, Yigang Wang","doi":"10.1002/pdi3.2501","DOIUrl":"10.1002/pdi3.2501","url":null,"abstract":"Despite advances in prenatal screening and a notable decrease in mortality rates, congenital heart disease (CHD) remains the most prevalent congenital disorder in newborns globally. Current therapeutic surgical approaches face challenges due to the significant rise in complications and disabilities. Emerging cardiac regenerative therapies offer promising adjuncts for CHD treatment. One novel avenue involves investigating methods to stimulate cardiomyocyte proliferation. However, the mechanism of altered cardiomyocyte proliferation in CHD is not fully understood, and there are few feasible approaches to stimulate cardiomyocyte cell cycling for optimal healing in CHD patients. In this review, we explore recent progress in understanding genetic and epigenetic mechanisms underlying defective cardiomyocyte proliferation in CHD from development through birth. Targeting cell cycle pathways shows promise for enhancing cardiomyocyte cytokinesis, division, and regeneration to repair heart defects. Advancements in human disease modeling techniques, CRISPR-based genome and epigenome editing, and next-generation sequencing technologies will expedite the exploration of abnormal machinery governing cardiomyocyte differentiation, proliferation, and maturation across diverse genetic backgrounds of CHD. Ongoing studies on screening drugs that regulate cell cycling are poised to translate this nascent technology of enhancing cardiomyocyte proliferation into a new therapeutic paradigm for CHD surgical interventions.","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11412308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142306178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activated phosphoinositide 3-kinase delta syndrome: Pathogenesis, clinical manifestations, and treatment. 活化磷酸肌肽3-激酶δ综合征：发病机制、临床表现和治疗。

Pediatric discovery Pub Date : 2024-08-23 eCollection Date: 2024-09-01 DOI: 10.1002/pdi3.2504

Ke Zhu, Qifan Li, Lingli Han, Jinqiao Sun

{"title":"Activated phosphoinositide 3-kinase delta syndrome: Pathogenesis, clinical manifestations, and treatment.","authors":"Ke Zhu, Qifan Li, Lingli Han, Jinqiao Sun","doi":"10.1002/pdi3.2504","DOIUrl":"10.1002/pdi3.2504","url":null,"abstract":"Activated phosphoinositide 3-kinase delta syndrome (APDS) is an autosomal dominant inborn errors of immunity resulting from gain of function mutations in the PIK3CD gene or loss of function mutations in the PIK3R1 gene. These mutations lead to hyperactivation of the phosphoinositide 3-kinase/Akt/mammalian target of rapamycin (mTOR) signaling pathways, causing complex deficiencies in cellular and humoral immunity. APDS patients exhibit diverse clinical manifestations including recurrent respiratory infections, nonneoplastic lymphoproliferation, an increasing risk of malignancy, and autoimmune diseases. Neurodevelopmental abnormalities, short stature, failure to thrive, and psychological disorders are also observed. Management strategies for APDS involve antibiotic prophylaxis and immunoglobulin replacement therapy, immunosuppressive therapies, and hematopoietic stem cell transplantation for severe cases. Targeted therapies, such as the mTOR inhibitor sirolimus and the elective Phosphoinositide 3-kinase delta inhibitor leniolisib, have emerged as promising options, demonstrating both safety and effectiveness. Continuous monitoring and further research are essential to optimize treatment strategies and understand the long-term implications of these interventions. This review aims to summarize the pathogenesis, clinical manifestations, and treatment of APDS.","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"2 3","pages":"e2504"},"PeriodicalIF":0.0,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth parameters and food frequently consumed by Basotho children aged 6-24 months old at Maseru and Leribe districts of Lesotho: A cross-sectional study. 莱索托马塞卢和莱里贝地区6-24个月大的巴索托儿童的生长参数和经常食用的食物：一项横断面研究。

Pediatric discovery Pub Date : 2024-08-13 eCollection Date: 2025-03-01 DOI: 10.1002/pdi3.2503

Mothusi Nyofane, Malebusa Rapotsane, Mohlotsane Moshao

{"title":"Growth parameters and food frequently consumed by Basotho children aged 6-24 months old at Maseru and Leribe districts of Lesotho: A cross-sectional study.","authors":"Mothusi Nyofane, Malebusa Rapotsane, Mohlotsane Moshao","doi":"10.1002/pdi3.2503","DOIUrl":"10.1002/pdi3.2503","url":null,"abstract":"Undernutrition in children remains a public health concern. Despite the global efforts to address undernutrition, Lesotho continues to bear the highest burden of childhood undernutrition. The study assessed the anthropometric measurements and dietary intake of children aged 6-24 months. A descriptive cross-sectional study was conducted among 113 mother-child dyads attending clinic visits at Makoanyane Military Hospital (Maseru district); n = 50 and Motebang Hospital (Leribe district); n = 63. A structured sociodemographic and feeding practices questionnaires based on adapted World Health Organization (WHO) questionnaires were used. The usual food consumption was collected using an unquantified food frequency questionnaire. Anthropometric measurements and z scores computation were done as per WHO standard guidelines. Statistics included percentages for categorical variables and means for continuous variables. The percentage of continued breastfeeding was 54.0% in Maseru and 28.6% in Leribe districts. Complementary feeds were introduced at the mean age of 5.3 ± 1.0 (Maseru) and 5.2 ± 1.3 months (Leribe). In Leribe, 84.1% of children were consuming maize porridge every day while in Maseru, 68.0% of children were consuming commercial baby cereal every day. The prevalence of wasting was 10.0% (Maseru) and 20.6% with 14.3% of severe wasting (Leribe). A higher percentage of stunting was observed in Leribe (36.5%) than in Maseru (20.0%) (p < 0.001). The prevalence of moderate and severe stunting was 8.0% and 12.0% in Maseru and 20.6% and 15.9% in Leribe, respectively. The prevalence of stunting is alarmingly high in Leribe. The findings suggest an urgent need to strengthen maternal and child health and nutrition programs to ameliorate feeding practices and nutritional status.","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"3 1","pages":"e2503"},"PeriodicalIF":0.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-way valves in breathing tubing reduce dead space during spontaneous breathing in anesthetized piglets. 呼吸管中的单向阀减少麻醉仔猪自主呼吸时的死亡空间。

Pediatric discovery Pub Date : 2024-08-04 eCollection Date: 2024-09-01 DOI: 10.1002/pdi3.2502

Pan Li, Weiping Wang, Wen Gao, Yanling Tan, Yu Hu, Li Jiang

{"title":"One-way valves in breathing tubing reduce dead space during spontaneous breathing in anesthetized piglets.","authors":"Pan Li, Weiping Wang, Wen Gao, Yanling Tan, Yu Hu, Li Jiang","doi":"10.1002/pdi3.2502","DOIUrl":"10.1002/pdi3.2502","url":null,"abstract":"The circle breathing system was unsafe for spontaneous breathing because of hypercapnia during anesthesia. Few studies have examined the minimizing dead space in breathing tubing. This study investigated one-way valves in the breathing tubing during spontaneous breathing in piglets. Six female piglets aged 68-71 days spontaneously breathed sevoflurane for 4 h randomly via traditional or anti-rebreathing tubing. Arterial carbon dioxide tension (paCO2) and respiratory characteristics were used to assess spontaneous breathing efficiency. mRNA-based methods, immunohistochemistry, and histology were used to assess the lungs. After induction, all piglets had mild hypercapnia. Those who breathed via traditional tubing experienced severe hypercapnia and required assisted ventilation (mean [95% confidence interval for mean]: 3 [0.5; 5.5] times) over 4 h. Piglets who breathed via anti-rebreathing tubing were able to normalize without assisted ventilation in less than 3 h and maintained. paCO2 was higher in the traditional group than the anti-rebreathing group at 3 and 4 hours (46.3 [42.1; 50.5] vs. 38.3 [34.1; 42.5] mmHg, p = 0.020; 46.3 [42.6; 50.0] vs. 40.7 [37.0; 44.4] mmHg, p = 0.040). However, one-way valves increased resistance to breathing. For the lungs, mRNA-based methods indicated higher expressions of cyclin-dependent kinase, cell division cycle 20, and cyclin B2 in the traditional group; immunohistochemistry identified higher expression of phosphorylated histone 2AX in the traditional group; histology showed similar damage between the groups. These findings suggest that one-way valves inside breathing tubing reduced dead space during spontaneous breathing and enhanced inhalation anesthesia advantages in the circle breathing system.","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"2 3","pages":"e2502"},"PeriodicalIF":0.0,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astrocytic connexin 43-hemichannels aggravate seizures by modulating blood-brain barrier permeability in temporal lobe epilepsy mice. 星形细胞连接蛋白43半通道通过调节颞叶癫痫小鼠血脑屏障通透性加重癫痫发作。

Pediatric discovery Pub Date : 2024-08-02 eCollection Date: 2024-09-01 DOI: 10.1002/pdi3.2500

Honghong Song, Yi Li, Jiayu Liu, Xianshu Bai, Li Jiang, Tingsong Li

{"title":"Astrocytic connexin 43-hemichannels aggravate seizures by modulating blood-brain barrier permeability in temporal lobe epilepsy mice.","authors":"Honghong Song, Yi Li, Jiayu Liu, Xianshu Bai, Li Jiang, Tingsong Li","doi":"10.1002/pdi3.2500","DOIUrl":"10.1002/pdi3.2500","url":null,"abstract":"The involvement of astrocytic connexin 43 (Cx43) in epileptogenesis has been extensively studied through various approaches, yet the underlying mechanism remains enigmatic. In this study, we explored whether astrocytic Cx43 forms hemichannels (HCs) that contribute to seizure progression in temporal lobe epilepsy (TLE) in mice. We focused on how these HCs influence the permeability of the blood-brain barrier (BBB), a crucial factor in the pathophysiology of epilepsy. Immunofluorescence staining and western blot analysis were employed to assess Cx43 expression in kainic acid-induced TLE mice, while BBB permeability was evaluated in TLE mice and those treated with TAT-Gap19 (an astrocytic Cx43 HC inhibitor) using Evans Blue permeation, serum S100β protein quantification, ZO-1 expression, and albumin extravasation into brain parenchyma via western blotting. Furthermore, seizure burden was monitored continuously using telemetric electroencephalography (EEG) and video monitoring in epileptic and TAT-Gap19-treated mice. Results demonstrated a significant increase in Cx43 content in hippocampal tissue in the TLE group, with a pronounced expression around blood vessels. TAT-GAP19 treatment alleviated EEG seizures and BBB permeability in TLE mice. These findings suggest that astrocytic Cx43 HCs in the hippocampus play a crucial role in epileptogenesis and seizure progression by regulating BBB permeability. Targeting Cx43-formed HCs distributed around the neurovascular unit may offer a novel therapeutic approach for epilepsy.","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"2 3","pages":"e2500"},"PeriodicalIF":0.0,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of ribonucleotide reductase M2 as a novel prognostic maker and therapeutic target to inhibit medulloblastoma proliferation, migration, and invasiveness. 核糖核苷酸还原酶M2作为抑制成神经管细胞瘤增殖、迁移和侵袭性的新预后因子和治疗靶点的潜力。

Pediatric discovery Pub Date : 2024-07-31 eCollection Date: 2024-09-01 DOI: 10.1002/pdi3.81

Xuanxuan Wu, Pan Gou, Chencheng Fang, Yudong Zhou, Lusheng Li, Xuan Zhai, Ping Liang

{"title":"The potential of ribonucleotide reductase M2 as a novel prognostic maker and therapeutic target to inhibit medulloblastoma proliferation, migration, and invasiveness.","authors":"Xuanxuan Wu, Pan Gou, Chencheng Fang, Yudong Zhou, Lusheng Li, Xuan Zhai, Ping Liang","doi":"10.1002/pdi3.81","DOIUrl":"10.1002/pdi3.81","url":null,"abstract":"The crucial role of ribonucleotide reductase M2 (RRM2) enzyme in cancer occurrence and progression has been well-established, but its specific function and significance in medulloblastoma (MB) remains largely unknown. First, we conducted a bioinformatics analysis of public genomic databases and observed highly expressed RRM2 in MB and an association of high RRM2 expression with adverse outcomes. In addition, by collecting clinical MB specimens for polymerase chain reaction (PCR), western blotting (WB), and immunohistochemistry (IHC), RRM2 was confirmed to be highly expressed in tumor tissues. Furthermore, immunohistochemical analysis linked adverse prognosis to high RRM2 expression. Moreover, knocking down RRM2 significantly inhibited MB cell proliferation, migration, and invasion in vitro. This report is the first to demonstrate the oncogenic role of RRM2 in MB, associated with adverse patient outcomes. Knocking down RRM2 contributes to weakened proliferating, migrating, and invading potentials of MB cells. RRM2 is expected to be a novel prognostic biomarker and therapeutic target for MB.","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"2 3","pages":"e81"},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide donor S-Nitroso-N-acetyl penicillamine for hepatic stellate cells to restore quiescence. 一氧化氮供体s -亚硝基- n -乙酰青霉胺用于肝星状细胞恢复静止。

Pediatric discovery Pub Date : 2024-06-28 eCollection Date: 2024-12-01 DOI: 10.1002/pdi3.95

Junbao Du, Yin He, Wen Jia, Xiaohua Liang, Quan Kang, Yun He

{"title":"Nitric oxide donor S-Nitroso-N-acetyl penicillamine for hepatic stellate cells to restore quiescence.","authors":"Junbao Du, Yin He, Wen Jia, Xiaohua Liang, Quan Kang, Yun He","doi":"10.1002/pdi3.95","DOIUrl":"10.1002/pdi3.95","url":null,"abstract":"Liver fibrosis is a hepatic scar repair response associated with a wide range of liver injuries, which is mediated by an imbalance between extracellular matrix (ECM) synthesis and degradation, leading to massive ECM deposition and disruption of normal liver architecture. Hepatic stellate cells (HSCs) are the main source of ECM during liver fibrosis and are the first identified cell subpopulation associated with liver fibrosis formation. Various current studies on the mechanism and treatment of liver fibrosis require resting-state HSCs as study subjects. However, spontaneous activation of primary HSCs occurs after 2-3 days of culture after isolation, and it is common that HSCs cell lines gradually differentiate into fibroblasts with culture time. This study provides an induction medium for quiescent HSCs-containing all-trans retinoic acid, sodium oleate, and S-nitroso-N-acetyl penicillamine (SNAP)-and an induction method. The induction method not only maintains the HSCs cell line in a quiescent state but also restores the activated HSCs to a quiescent state. The method has a good induction effect, short induction time, and convenient operation, which is worth being popularized and used in a wide range of laboratories.","PeriodicalId":520221,"journal":{"name":"Pediatric discovery","volume":"2 4","pages":"e95"},"PeriodicalIF":0.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0