Advanced biotechnology最新文献_第3页

Microbial biotechnology: from synthetic biology to synthetic ecology. 微生物生物技术：从合成生物学到合成生态学。

Advanced biotechnology Pub Date : 2025-01-03 DOI: 10.1007/s44307-024-00054-4

Qingyun Yan, Yuzhen Ming, Jianzhong Liu, Huaqun Yin, Qiang He, Juan Li, Mingtao Huang, Zhili He

引用次数: 0

Therapeutic gene correction of HBB frameshift CD41-42 (-TCTT) deletion in human hematopoietic stem cells. 人造血干细胞HBB移码CD41-42 （-TCTT）缺失的治疗性基因校正

Advanced biotechnology Pub Date : 2025-01-02 DOI: 10.1007/s44307-024-00053-5

Qianyi Liu, Xinyu Li, Hui Xu, Ying Luo, Lin Cheng, Junbin Liang, Yuelin He, Haiying Liu, Jianpei Fang, Junjiu Huang

{"title":"Therapeutic gene correction of HBB frameshift CD41-42 (-TCTT) deletion in human hematopoietic stem cells.","authors":"Qianyi Liu, Xinyu Li, Hui Xu, Ying Luo, Lin Cheng, Junbin Liang, Yuelin He, Haiying Liu, Jianpei Fang, Junjiu Huang","doi":"10.1007/s44307-024-00053-5","DOIUrl":"10.1007/s44307-024-00053-5","url":null,"abstract":"Β-thalassemia is one of the global health burdens. The CD41-42 (-TCTT) mutation at HBB is the most prevalent pathogenic mutation of β-thalassemia in both China and Southeast Asia. Previous studies focused on repairing the HBB CD41-42 (-TCTT) mutation in β-thalassemia patient-specific induced pluripotent stem cells, which were subsequently differentiated into hematopoietic stem and progenitor cells (HSPCs) for transplantation. In this study, we directly applied the CRISPR/Cas9-based gene editing therapy to correct the HBB CD41-42 (-TCTT) mutation in patient-derived HSPCs. The effective editing induced by Cas9:sgRNA ribonucleoprotein and single-stranded oligodeoxynucleotides (ssODNs) was confirmed in HUDEP-2 cell lines harboring the HBB CD41-42 (-TCTT) mutation. Further correction of heterozygote and homozygote HBB CD41-42 (-TCTT) mutations in patient-derived HSPCs resulted in a 13.4-40.8% increase in the proportion of HBB-expressing (HBB +) cells following erythroid differentiation in vitro. At 16 weeks post-xenotransplantation of the edited HSPCs into coisogenic immunodeficient mice, the reparation efficiency in engrafted bone marrow was 17.21% ± 3.66%. Multiparameter flow cytometric analysis of the engrafted bone marrow showed an increase in the percentage of HBB + cells without impairing the ability of engraftment, self-renewal, and multilineage hematopoietic repopulation of HSPCs. For the safety evaluation, 103 potential off-target sites were predicted by SITE-seq and CRISPOR, with one site displaying significant off-target editing. Since this off-target site is located in the intergenic region, it is presumed to pose minimal risk. Taken together, our study provides critical preclinical data supporting the safety and efficacy of the gene therapy approach for HBB CD41-42 (-TCTT) mutation.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"3 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio-nanopore technology for biomolecules detection. 生物分子检测的生物纳米孔技术。

Advanced biotechnology Pub Date : 2024-12-06 DOI: 10.1007/s44307-024-00051-7

Peizhi Li, Dan Liang, En Yang, Mustafa Zeb, Huiqi Huang, Haihui Sun, Wenhan Zhang, Chifang Peng, Yuan Zhao, Wei Ma

引用次数: 0

Defense guard: strategies of plants in the fight against Cadmium stress. 防御防御：植物对抗镉胁迫的策略。

Advanced biotechnology Pub Date : 2024-12-02 DOI: 10.1007/s44307-024-00052-6

Qian-Hui Zhang, Yi-Qi Chen, Zhen-Bang Li, Xuan-Tong Tan, Guo-Rong Xin, Chun-Tao He

引用次数: 0

Roots to the rescue: how plants harness hydraulic redistribution to survive drought across contrasting soil textures. 根系救援：植物如何利用水力再分配在不同土壤质地的干旱中生存。

Advanced biotechnology Pub Date : 2024-11-25 DOI: 10.1007/s44307-024-00050-8

Shenglan Sha, Gaochao Cai, Shurong Liu, Mutez Ali Ahmed

{"title":"Roots to the rescue: how plants harness hydraulic redistribution to survive drought across contrasting soil textures.","authors":"Shenglan Sha, Gaochao Cai, Shurong Liu, Mutez Ali Ahmed","doi":"10.1007/s44307-024-00050-8","DOIUrl":"10.1007/s44307-024-00050-8","url":null,"abstract":"Hydraulic redistribution (HR) is a critical ecological process whereby plant roots transfer water from wetter to drier soil layers, significantly impacting soil moisture dynamics and plant water and nutrient uptake. Yet a comprehensive understanding of the mechanism triggering HR and its influencing factors remains elusive. Here, we conducted a systematic meta-analysis to discuss the influence of soil conditions and plant species characteristics on HR occurrence. The threshold of HR ranges from -1.80 to -0.05 MPa, with soil hydraulic conductivity between 1.51 × 10-13 and 6.53 × 10-5 cm s-1 when HR occurs. HR is influenced by various factors. Soil texture plays a pivotal role, with loamy soils promoting HR more effectively than sandy and clay soils. Plant root structure and hydraulic conductivity significantly influence HR occurrence, where HR is more prevalent in deep-rooted tree species with larger root canal diameters and dimorphic roots. Additionally, mycorrhizal fungi enhance HR by expanding root uptake area, reducing water transport distances and improving soil structure. However, adverse soil conditions, inadequate plant physiological regulatory capacity, or methodological limitations can hinder HR detection. The findings highlight that HR is more likely to occur where there is a significant water potential gradient, appropriate root-soil contact, and low nocturnal transpiration. Plants can effectively replenish the water in dry root systems under drought conditions by HR by increasing the water potential of root systems to maintain normal physiological functions. Our study identifies key factors influencing HR, offering a comprehensive framework for future research aimed at improving plant drought resistance and refining ecohydrological models.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"2 4","pages":"43"},"PeriodicalIF":0.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saving coral reefs: significance and biotechnological approaches for coral conservation. 拯救珊瑚礁：珊瑚保育的意义及生物技术方法。

Advanced biotechnology Pub Date : 2024-11-22 DOI: 10.1007/s44307-024-00049-1

Pansa Cecchini, Thomas Nitta, Edoardo Sena, Zhi-Yan Du

引用次数: 0

The inhibitory effect of Hypericum japonicum on H9N2 avian influenza virus. 金丝桃对H9N2禽流感病毒的抑制作用。

Advanced biotechnology Pub Date : 2024-11-06 DOI: 10.1007/s44307-024-00046-4

Huiqiong Hu, Jingmin Li, Shumei Zheng, Keyu Zhao, Yongbo Xia, Xiaona Wei, Mingzheng Han, Yukun Zhao, Ouyang Peng, Zhiqing Zhao, Zexin Chen, Weiwei Su, Yongchang Cao, Yonggang Wang, Chunyi Xue

{"title":"The inhibitory effect of Hypericum japonicum on H9N2 avian influenza virus.","authors":"Huiqiong Hu, Jingmin Li, Shumei Zheng, Keyu Zhao, Yongbo Xia, Xiaona Wei, Mingzheng Han, Yukun Zhao, Ouyang Peng, Zhiqing Zhao, Zexin Chen, Weiwei Su, Yongchang Cao, Yonggang Wang, Chunyi Xue","doi":"10.1007/s44307-024-00046-4","DOIUrl":"10.1007/s44307-024-00046-4","url":null,"abstract":"The H9N2 subtype of avian influenza virus (AIV) causes severe immunosuppression and high mortality in view of its frequent co-infection with other pathogens, resulting in significant economic losses in the poultry industry. Current vaccines provide suboptimal immune protection against H9N2 AIV owing to antigenic variations, highlighting the urgent need for safe and effective antiviral drugs for the prevention and treatment of this virus. This study aimed to investigate the inhibitory effects of Hypericum japonicum extract on H9N2 AIV. Our findings revealed that the extract obtained through resin column separation using 60% ethanol (S06-60%) inhibited H9N2 AIV replication in Madin-Daby canine kidney cells in a dose-dependent manner. The maximum safe concentration of the water-soluble S06-60% extract was determined to be 0.05 mg/mL. Time-course experiments indicated that S06-60% primarily exerted its antiviral effects during the viral pretreatment and adsorption stages. Furthermore, in vivo experiments conducted on specific pathogen-free chickens confirmed the effectiveness of S06-60% in inhibiting H9N2 AIV infection and mitigating associated damage to tracheal and lung tissues. Overall, our study highlights the therapeutic potential of Hypericum japonicum extract S06-60% as a viable antiviral candidate against H9N2 AIV, offering promising implications for its application in poultry health management to reduce the economic impact on the poultry industry.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"2 4","pages":"41"},"PeriodicalIF":0.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When agriculture meets biotechnology: a route for future agricultural innovation. 当农业遇上生物技术：未来农业创新之路。

Advanced biotechnology Pub Date : 2024-11-01 DOI: 10.1007/s44307-024-00047-3

Jun-Peng Shi, Ruo-Han Xie, Fang Yang, Wang Zhou, Xiao-Qian Jiang, Hai-Fei Hu, Chao Yang, Yong-Yao Xie, Shi Xiao

引用次数: 0

A century of innovation in life sciences at Sun Yat-sen University. 中山大学生命科学百年创新。

Advanced biotechnology Pub Date : 2024-11-01 DOI: 10.1007/s44307-024-00048-2

Jianhua Yang, Shi Xiao

引用次数: 0

Advances in biotechnology and breeding innovations in China's marine aquaculture. 中国海洋水产养殖生物技术与育种创新进展。

Advanced biotechnology Pub Date : 2024-10-18 DOI: 10.1007/s44307-024-00043-7

Wenteng Xu, Yang Liu, Ming Li, Sheng Lu, Songlin Chen

引用次数: 0