Advanced biotechnology最新文献_第6页

Microbacterium testaceum facilitates polysaccharide decomposition during post-harvest aging of tobacco leaves by recruiting keystone bacterial taxa. 睾丸微杆菌通过招募关键细菌类群促进烟叶采收后老化过程中多糖的分解。

Advanced biotechnology Pub Date : 2025-11-19 DOI: 10.1007/s44307-025-00086-4

Yichao Hu, Yuwen Wang, Tian Qin, Weihao Chen, Tingting Ma, Jia Lei, Qinlin Fu, Xingpeng Feng, Zhiwei Han, Juan Li

{"title":"Microbacterium testaceum facilitates polysaccharide decomposition during post-harvest aging of tobacco leaves by recruiting keystone bacterial taxa.","authors":"Yichao Hu, Yuwen Wang, Tian Qin, Weihao Chen, Tingting Ma, Jia Lei, Qinlin Fu, Xingpeng Feng, Zhiwei Han, Juan Li","doi":"10.1007/s44307-025-00086-4","DOIUrl":"10.1007/s44307-025-00086-4","url":null,"abstract":"The use of functional microorganisms is a widely adopted, green, and efficient industrial technique for enhancing tobacco leaf quality. These microorganisms accelerate the degradation of macromolecular organic substances. However, their impact on the chemical composition of tobacco leaves across different aging durations and the mechanisms of polysaccharide degradation remain unclear. This study analyzed the degradation patterns of starch, pectin, cellulose, and hemicellulose at different time points (3 h, 2 months, 6 months, 36 months) during the tobacco aging process after the addition of Microbacterium testaceum, and compared the differences in microbial community structure, diversity, and molecular ecological networks. The results showed that compared with sterile water treatment of the tobacco leaf aging process, the exogenous addition of Microbacterium testaceum accelerated the degradation of polysaccharide macromolecules, and the highest degradation rate of starch was 17.4% at the aging stage of 2-6 months, and the highest degradation rate of pectin was 45.46% at the aging stage of 6-36 months. At the same time, the exogenous addition of Microbacterium testaceum altered the microbial community structure during the tobacco aging process by increasing the number of core functional microorganisms, such as Delftia and Proteus, which promoted microorganisms that play a role in material degradation in the ecological environment of tobacco aging. This study provided a theoretical basis for the regulation of interspecific microbial interactions by exogenous functional strains over a broad timescale (from 3 h to 36 months) during tobacco aging, thereby promoting the degradation of polysaccharide macromolecules.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"3 4","pages":"32"},"PeriodicalIF":0.0,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12627313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145552631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-exposure to microplastics and bisphenol A increases viral susceptibility in largemouth bass (Micropterus salmoides) via oxidative stress. 共同暴露于微塑料和双酚A通过氧化应激增加了大口黑鲈（Micropterus salmoides）的病毒敏感性。

Advanced biotechnology Pub Date : 2025-11-06 DOI: 10.1007/s44307-025-00085-5

Jie Gao, Junzhe Zhang, Rui Zheng, Jing Jiang, Siyou Huang, Qijin Miao, Bingya Wu, Wanting Tang, Jianguo He, Junfeng Xie

{"title":"Co-exposure to microplastics and bisphenol A increases viral susceptibility in largemouth bass (Micropterus salmoides) via oxidative stress.","authors":"Jie Gao, Junzhe Zhang, Rui Zheng, Jing Jiang, Siyou Huang, Qijin Miao, Bingya Wu, Wanting Tang, Jianguo He, Junfeng Xie","doi":"10.1007/s44307-025-00085-5","DOIUrl":"10.1007/s44307-025-00085-5","url":null,"abstract":"Microplastics (MPs) and bisphenol A (BPA) frequently co-occur in freshwater ecosystems, yet their combined impacts on host-pathogen interactions remain poorly understood. Here, we exposed juvenile largemouth bass (Micropterus salmoides) to environmentally relevant concentrations of MPs, BPA, and their mixture. Co-exposure markedly inhibited NRF2-mediated antioxidant signaling, leading to downregulation of antioxidant enzymes (SOD1, CAT, GPx), elevated hepatic reactive oxygen species and malondialdehyde, and depletion of ATP. These redox disturbances were accompanied by mitochondrial damage, increased expression of pro-apoptotic genes (Bax, Caspase-3), and accumulation of TUNEL-positive nuclei, indicative of apoptosis. Strikingly, only co-exposed fish exhibited enhanced replication of nervous necrosis virus (NNV), a response absent under single exposures. Our findings demonstrate that MPs and BPA act synergistically to disrupt redox homeostasis and compromise antiviral defense, thereby heightening viral susceptibility in a freshwater aquaculture species. This study highlights the overlooked infection risks posed by pollutant mixtures and emphasizes the need to incorporate mixture toxicity into freshwater ecotoxicological risk assessments.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"3 4","pages":"31"},"PeriodicalIF":0.0,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12592606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145454528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct detection of meiotic recombination events in the highly heterozygous amphioxus genome. 高度杂合文昌鱼基因组减数分裂重组事件的直接检测。

Advanced biotechnology Pub Date : 2025-10-06 DOI: 10.1007/s44307-025-00083-7

Lei Tao, Jing Xue, Junwei Cao, Guang Li, Cai Li

{"title":"Direct detection of meiotic recombination events in the highly heterozygous amphioxus genome.","authors":"Lei Tao, Jing Xue, Junwei Cao, Guang Li, Cai Li","doi":"10.1007/s44307-025-00083-7","DOIUrl":"10.1007/s44307-025-00083-7","url":null,"abstract":"Amphioxus, a basal chordate with highly heterozygous genomes (3.2 ~ 4.2% in sequenced species), represents a key model for understanding vertebrate origins. However, the extreme heterozygosity poses challenges for many genomic analyses, including studying meiotic recombination. Here, we present a novel bioinformatic pipeline that enables direct detection of crossover (CO) and non-crossover (NCO) recombination events using short-read whole-genome sequencing of a two-generation pedigree (two parents and 104 F1 offspring) of the amphioxus Branchiostoma floridae. Using parental assemblies generated by Platanus-allee as a custom reference for read alignment, we tracked inheritance patterns in offspring and phased contig-level haplotypes in parents, allowing us to detect recombination events. We identified 2,329 paternal and 2,288 maternal COs, yielding recombination rates of 4.66 cM/Mb and 4.57 cM/Mb, respectively. We found CO coldspots spanning > 140 Mb in each parent and these are likely associated with large-scale heterozygous inversions. CO rates were positively correlated with transposable element and gene density in both sexes, but showed weak or no correlation with GC content. We further identified ~ 10,000 paternal and ~ 5,800 maternal NCO events, predominantly shorter than 200 bp in tract length, and found evidence of GC-biased gene conversion. This work provides the first direct and genome-wide measurement of recombination in amphioxus and demonstrates how high heterozygosity, often considered a barrier, can be leveraged for fine-scale recombination mapping. Our findings illuminate conserved and divergent features of recombination in chordates and establish a framework for studying recombination in other highly heterozygous organisms.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"3 4","pages":"30"},"PeriodicalIF":0.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12500494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated proteomics and transcriptomics analysis of dynamic changes in muscle fiber types in different regions of porcine skeletal muscle. 猪骨骼肌不同区域肌纤维类型动态变化的综合蛋白质组学和转录组学分析。

Advanced biotechnology Pub Date : 2025-09-24 DOI: 10.1007/s44307-025-00080-w

Zhiting Feng, Xiaoyu Wang, Qingshuang Zhou, Yihao Liu, Rong Xu, Ziyun Liang, Chong Zhang, Xiaohong Liu, Yunxiang Zhao, Yaosheng Chen, Delin Mo

{"title":"Integrated proteomics and transcriptomics analysis of dynamic changes in muscle fiber types in different regions of porcine skeletal muscle.","authors":"Zhiting Feng, Xiaoyu Wang, Qingshuang Zhou, Yihao Liu, Rong Xu, Ziyun Liang, Chong Zhang, Xiaohong Liu, Yunxiang Zhao, Yaosheng Chen, Delin Mo","doi":"10.1007/s44307-025-00080-w","DOIUrl":"10.1007/s44307-025-00080-w","url":null,"abstract":"Skeletal muscle serves as a valuable source of nutrition, with distinct muscle fiber types exhibiting different physicochemical properties that influence both meat quality and muscle function. Bama miniature pigs (BM) are recognized for their superior meat quality and their relevance as models for human medical research. Therefore, investigating the differences between slow and fast muscles at various developmental stages (from 57 days post-fertilization to 120 days postnatally) in BM is crucial for both the pork industry and biomedical studies. In this study, we employed a non-targeted data-independent acquisition (nDIA) -based proteomic approach for the first time to porcine embryonic skeletal muscle fibers. A total of 616 differentially expressed genes (DEGs) and 272 differentially abundant proteins (DAPs) were identified in the fast-twitch longissimus dorsi (LD) and slow-twitch semitendinosus (SD) muscles of BM. Domain enrichment analysis and in vitro experiments demonstrated that the NEK3 gene, containing the S_TKc domain, inhibits fast-twitch muscle fiber differentiation postnatally. Additionally, cross-species analysis showed upregulation of skeletal muscle development organ genes in pigs at postnatal day 28. In summary, our results provide both fundamental data and novel insights to further uncover the mechanisms underlying pig skeletal muscle development and muscle fiber transition.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"3 4","pages":"29"},"PeriodicalIF":0.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced biotechnology Pub Date : 2025-09-23 DOI: 10.1007/s44307-025-00081-9

Jia-Yi Luo, Ju-Feng Wang, Jiong Yang, Peng Zhang, Geoff Hide, De-Hua Lai, Zhao-Rong Lun

{"title":"Thermo-related degeneration of stumpy forms of Trypanosoma brucei, the pathogen of African sleeping sickness.","authors":"Jia-Yi Luo, Ju-Feng Wang, Jiong Yang, Peng Zhang, Geoff Hide, De-Hua Lai, Zhao-Rong Lun","doi":"10.1007/s44307-025-00081-9","DOIUrl":"10.1007/s44307-025-00081-9","url":null,"abstract":"Trypanosoma brucei, the causative agent of African trypanosomiasis, develops from the long slender (LS) to the short stumpy (SS) form in the mammalian host. The SS trypanosomes are critical for transmission to the insect vector but face significant challenges within the vertebrate host. The role of the immune response in controlling the parasitaemia is well studied, however, the mechanism underpinning the rapid degeneration of SS trypanosomes during the first parasitaemic peak in mice remains somewhat elusive. We demonstrate that fever is a critical yet underexplored factor in facilitating the clearance of SS trypanosomes, suggesting that temperature may play a critical role in regulating the natural turnover of SS trypanosomes. The elevated body temperature correlates with the parasitaemic dynamics, accelerating SS trypanosome elimination in the mammalian host. The SS trypanosomes exhibited high thermo-sensitivity to elevated temperatures, accompanied with apoptosis-like events, mitochondrial damage and oxidative stress. Metabolomic profiling also revealed disruptions in glycolysis and the TCA cycle, shedding light on the processes in compromising the SS trypanosomes. Interestingly, antibodies during the acute phase did not directly cause SS trypanosomes death, but the combination of elevated temperature and antibodies enhanced the clearance of SS trypanosomes, highlighting the critical role of fever in eliminating the first parasitaemic peak. Our findings detail the mechanism of vulnerability of SS trypanosome to elevated temperatures and suggest that host fever serves as a neglected, but critical mechanism, for T. brucei SS trypanosome clearance.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"3 4","pages":"28"},"PeriodicalIF":0.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering essential lncRNAs through transcriptome-scale CRISPR-Cas13 screening. 通过转录组级CRISPR-Cas13筛选发现必要的lncrna。

Advanced biotechnology Pub Date : 2025-09-15 DOI: 10.1007/s44307-025-00082-8

Jiahua Si, Xinming Su, Zhuoyan Jin, Shiwei Duan

{"title":"Uncovering essential lncRNAs through transcriptome-scale CRISPR-Cas13 screening.","authors":"Jiahua Si, Xinming Su, Zhuoyan Jin, Shiwei Duan","doi":"10.1007/s44307-025-00082-8","DOIUrl":"10.1007/s44307-025-00082-8","url":null,"abstract":"Approximately 75% of the human genome is transcribed into RNA, yet less than 5% encodes proteins, with the majority producing non-coding RNAs (ncRNAs). Among them, long non-coding RNAs (lncRNAs) represent a major class that exerts broad regulatory influence across cellular processes, disease contexts, and developmental stages. Despite their potential as biomarkers and therapeutic targets, their low sequence conservation, limited abundance, and structural complexity present significant challenges for functional characterization. Traditional RNA interference and CRISPR-Cas9-based methods have offered partial insights but remain limited in efficiency, specificity, and scalability. To address these barriers, Neville E. Sanjana's team developed CaRPool-seq, a transcriptome-scale CRISPR-Cas13 screening platform that directly targets RNA. Applying this approach across diverse human cell lines, they identified 778 essential lncRNAs, including 46 universally required for survival, with distinctive structural features and functional independence from neighboring protein-coding genes. Integration with single-cell transcriptomics revealed their critical roles in cell-cycle regulation, apoptosis, and developmental gene expression, as well as aberrant expression patterns in cancer linked to patient outcomes. This study establishes CRISPR-Cas13 as a precise and scalable strategy for lncRNA functional discovery, expanding opportunities for biomarker identification, therapeutic development, and precision medicine.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"3 3","pages":"27"},"PeriodicalIF":0.0,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of AarMIXTAs as essential regulators in T-shaped non-glandular trichome development of Artemisia argyi. armixtas在艾叶t型非腺毛发育中的功能特征

Advanced biotechnology Pub Date : 2025-09-12 DOI: 10.1007/s44307-025-00077-5

Xinlian Chen, Duan Wu, Chunyu Li, Baosheng Liao, Qi Shen

引用次数: 0

Arabidopsis MACP2 contributes to autophagy induction by modulating starvation-induced reactive oxygen species homeostasis. 拟南芥MACP2通过调节饥饿诱导的活性氧稳态参与自噬诱导。

Advanced biotechnology Pub Date : 2025-08-20 DOI: 10.1007/s44307-025-00078-4

Ying Zhou, Xue Zhang, Tiancong Qi, Zi-Han Wang, Yao Wang, Lin-Na Wang, Yong-Lun Zeng, Hanjie He, Liwen Jiang, Daoxin Xie, Shi Xiao, Lu-Jun Yu, Qin-Fang Chen

{"title":"Arabidopsis MACP2 contributes to autophagy induction by modulating starvation-induced reactive oxygen species homeostasis.","authors":"Ying Zhou, Xue Zhang, Tiancong Qi, Zi-Han Wang, Yao Wang, Lin-Na Wang, Yong-Lun Zeng, Hanjie He, Liwen Jiang, Daoxin Xie, Shi Xiao, Lu-Jun Yu, Qin-Fang Chen","doi":"10.1007/s44307-025-00078-4","DOIUrl":"10.1007/s44307-025-00078-4","url":null,"abstract":"In plants, autophagy is a conserved recycling system essential for development and stress responses by targeting cellular components for massive degradation in the vacuole. Our previous work suggested that autophagy contributes to Arabidopsis (Arabidopsis thaliana) stress responses by modulating NADPH-oxidase-mediated reactive oxygen species (ROS) homeostasis; however, the molecular link between extracellular ROS and autophagy remains unknown. We performed a yeast two-hybrid screen to identify components involved in autophagy, using the central autophagy component ATG8e as a bait. We identified MEMBRANE ATTACK COMPLEX/PERFORIN-LIKE 2 (MACP2) as an interactor of ATG8e via its the ATG8-interacting motif and confirmed this interaction by co-immunoprecipitation and bimolecular fluorescence complementation assays. MACP2-overexpressing lines showed enhanced sensitivity to nutritional starvation, accelerated leaf senescence, and increased hydrogen peroxide (H2O2) levels, resembling the phenotypes of atg mutants defective in autophagy. Conversely, macp2 knockouts exhibited diminished starvation-induced H2O2 accumulation and attenuated autophagosome formation and fully suppressed the starvation-hypersensitive phenotypes of the atg5-1 mutant. In particular, MACP2 was degraded through the autophagy machinery during prolonged starvation, suggesting a feedback regulatory mechanism for maintaining MACP2 homeostasis. Our findings suggest that MACP2 acts as a key regulator in autophagy induction by controlling influx of extracellular H2O2 in Arabidopsis.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"3 3","pages":"25"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144985237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering of autoactive NLRs: a big step toward breeding crops with durable and broad-spectrum resistance. 自动nlr的工程：向培育具有持久和广谱抗性的作物迈出了一大步。

Advanced biotechnology Pub Date : 2025-08-20 DOI: 10.1007/s44307-025-00079-3

Yan Wang, Shi Xiao

引用次数: 0

Mannose receptor RpMR1 of Manila clam (Ruditapes philippinarum) defense against Vibrio anguillarum infection. 马尼拉蛤抗鳗弧菌感染的甘露糖受体RpMR1。

Advanced biotechnology Pub Date : 2025-08-04 DOI: 10.1007/s44307-025-00075-7

Zhihui Yin, Hongtao Nie

{"title":"Mannose receptor RpMR1 of Manila clam (Ruditapes philippinarum) defense against Vibrio anguillarum infection.","authors":"Zhihui Yin, Hongtao Nie","doi":"10.1007/s44307-025-00075-7","DOIUrl":"10.1007/s44307-025-00075-7","url":null,"abstract":"The mannose receptor (MR) is a member of the C-type lectin superfamily and a type I transmembrane protein that functions as a pattern recognition receptor (PRR) in immune responses. In this study, we identified 13 MR genes (RpMR1-13) in the genome of Ruditapes philippinarum and investigated their expression profiles following Vibrio anguillarum challenge. Notably, RpMR1, RpMR2, RpMR3, and RpMR4 exhibited peak expression at 72 h post-infection. We successfully purified the recombinant RpMR1 protein and demonstrated its antibacterial activity against three Gram-negative bacteria (V. splendidus, V. alginolyticus, and V. anguillarum), though it had no effect on Gram-positive bacteria. Furthermore, in vivo injection of RpMR1 significantly reduced mortality in R. philippinarum following V. anguillarum infection. To explore role of RpMR1 in immune signaling, we performed RNA interference (dsRNA-RpMR1) and observed successful gene silencing. Subsequent qRT-PCR analysis revealed that RpMR1 knockdown significantly suppressed TLR4 expression (P< 0.05) under V. anguillarum stress, confirming an interaction between RpMR1 and TLR4 in the immune response. This study provides the first functional evidence of mannose receptor-mediated immunity in mollusks, offering new insights into the molecular defense mechanisms of R. philippinarum against bacterial infection.","PeriodicalId":519913,"journal":{"name":"Advanced biotechnology","volume":"3 3","pages":"23"},"PeriodicalIF":0.0,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12321717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144786270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0