Infectious diseases & clinical microbiology最新文献

{"title":"Cytomegalovirus Infections in Patients Diagnosed with Inflammatory Bowel Disease.","authors":"Melike İnan-Hekimoğlu, Elif Mukime Sarıcaoğlu, Güle Çınar, İrem Akdemir, Ezgi Gülten, Murat Törüner, Arzu Ensari, Berna Savaş, Kemal Osman Memikoğlu","doi":"10.36519/idcm.2024.370","DOIUrl":"10.36519/idcm.2024.370","url":null,"abstract":"<p><strong>Objective: </strong>The impact of cytomegalovirus (CMV) on inflammatory bowel disease (IBD) flares remains a matter of debate. This study aimed to evaluate patients with CMV infection who presented with IBD exacerbation in terms of diagnosis and treatment and investigate the importance of CMV DNA levels in colitis development.</p><p><strong>Materials and methods: </strong>Patients who were followed up with IBD and examined with clinical suspicion of CMV colitis at a university hospital between January 2016 and December 2021 were retrospectively scanned. This study included all patients who underwent colonoscopic biopsy with a preliminary diagnosis of CMV colitis and compared those with colitis detected histopathologically with those without colitis.</p><p><strong>Results: </strong>Thirty-nine patients with IBD were included in the study. No statistically significant difference was observed regarding the two groups' demographic data, clinical findings, and outcomes. The median serum CMV DNA level in patients with CMV colitis was 104 copies/mL, which was lower than in patients without colitis (1216 copies/mL) (<i>p</i>=0.008). Among patients with CMV colitis, CMV DNA levels were negative or low in 16 (61.5%).</p><p><strong>Conclusion: </strong>In patients with IBD, CMV colitis may not always be accompanied by CMV viremia. Therefore, negative or low serum CMV DNA levels are not sufficient to exclude CMV colitis. In case of high clinical suspicion, further examinations should be planned.</p>","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"282-290"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cystic Echinococcosis Cases: A Retrospective Evaluation in a General Surgery Department.","authors":"Oruç Numan Gökçe, Sevil Alkan, Volkan Karadağ","doi":"10.36519/idcm.2024.425","DOIUrl":"10.36519/idcm.2024.425","url":null,"abstract":"<p><strong>Objective: </strong>Cystic echinococcosis (CE) caused by the larval stage of the <i>Echinococcus granulosus</i> parasite is a global health problem. This study aimed to assess cases of CE admitted to our General Surgery Department retrospectively, as there is no known similar publication concerning surgical treatment of abdominal cystic echinococcosis in Çanakkale province.</p><p><strong>Materials and methods: </strong>We analyzed laboratory and radiological findings alongside clinical and demographic features, treatments, and outcomes of cases undergoing surgical treatment for abdominal cystic echinococcosis in our department between 2012 and 2022.</p><p><strong>Results: </strong>Among the 37 cases reviewed, the mean age was 45.59±7.1 years, and 19 (51.35%) were female. While the majority (54.05%) were from urban districts, only 37.84% were involved in animal husbandry and agriculture. Most common complaint was abdominal pain (67.57%), with an average symptom duration of 4.3±1.02 months. The diagnosis was confirmed by visualization of the cuticular membrane through pathological examination. <i>E. granulosus</i> IHA testing yielded positive in 29 cases. Spleen involvement was seen in 3 (8.11%) cases and isolated liver involvement in 34 (91.89%) cases. Gharbi type III cysts (n=15) were the most frequent. The average cyst size was 106.32 mm (range 50-200); 29 cases had solitary cysts, six had double cysts, and one had triple and quadruple cysts. Cystotomy with capitonage and laparoscopic pericystectomy were performed in 30 cases and seven cases, respectively. Our recurrence rate was low (2.7%). Early and late complications developed in 12 (32.43%) cases.</p><p><strong>Conclusion: </strong>Abdominal pain was the primary presenting symptom; radiology is valuable in diagnosis. Occupation in agriculture or animal husbandry is not the main risk factor in our region. Surgical interventions have favorable outcomes with low recurrence rates, particularly laparoscopic pericystectomy.</p>","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"291-297"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraventricular Tigecycline Therapy for Ventriculoperitoneal Shunt-Related Septic Shock Caused by Colistin-Resistant <i>Klebsiella pneumoniae</i>: A Case Report.","authors":"Tuğba Yanık-Yalçın, Fatma İrem Yeşiler, Helin Şahintürk, Salih Gülşen, Özlem Kurt-Azap, Pınar Zeyneloğlu","doi":"10.36519/idcm.2024.384","DOIUrl":"10.36519/idcm.2024.384","url":null,"abstract":"<p><p>Ventriculoperitoneal shunts (VPSs) have been proven to be life-saving procedures, but their complications pose challenges, particularly in this era of rising antibiotic resistance. We report a critically ill case with VPS infection due to colistin-resistant <i>Klebsiella pneumoniae</i> that was treated with intraventricular tigecycline as salvage therapy without adverse events, resulting in microbiologic cure and clinical response. The use of intraventricular tigecycline in the treatment of colistin-resistant <i>K. pneumoniae</i> appears promising; however, appropriate dosage adjustments and evidence-based recommendations are needed.</p>","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"328-333"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milestones and Momentum: Celebrating Six Years of Progress in <i>Infectious Diseases and Clinical Microbiology</i> (IDCM).","authors":"Murat Akova","doi":"10.36519/idcm.2024.523","DOIUrl":"https://doi.org/10.36519/idcm.2024.523","url":null,"abstract":"","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"257"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Diagnosis of Candidemia in the Intensive Care Unit by Clinical and Molecular Methods: A Prospective Observational Study.","authors":"Khalis Mustafayev, Mert Ahmet Kuşkucu, Fatma Nihan Akkoç-Mustafayev, Seval Ürkmez, Bilgül Mete, Gökhan Aygün","doi":"10.36519/idcm.2024.443","DOIUrl":"10.36519/idcm.2024.443","url":null,"abstract":"<p><strong>Objective: </strong>Early diagnosis and treatment of candidemia in intensive care units (ICUs) remain a significant challenge globally because of the lack of well-established non-culture-based diagnostic methods. This study aimed to evaluate risk factors in critically ill ICU patients, develop a unique <i>Candida</i> score, and create a real-time polymerase chain reaction (PCR) assay for the early diagnosis of candidemia.</p><p><strong>Materials and methods: </strong>The study was conducted in three phases: 1) Retrospective analysis of 100 ICU patients from İstanbul University-Cerrahpaşa between January 2017 and December 2018 to identify risk factors for invasive candidiasis, 2) development of Cerrahpaşa <i>Candida</i> score based on these findings, and 3) prospective evaluation of 75 ICU patients, applying the newly created Cerrahpaşa <i>Candida</i> score and implementing a rapid PCR-based test on whole blood samples. The PCR assay was part of a development study and was not used for scoring or clinical diagnostic evaluation. Specific primer pairs and EvaGreen® dye (Biotium Inc., USA) were used to differentiate between <i>Candida albicans</i> and <i>Candida parapsilosis</i> isolates through melting curve analysis of real-time PCR amplicons. The PCR test demonstrated a detection limit of 10 CFU/mL.</p><p><strong>Results: </strong>In the retrospective analysis, key risk factors for candidemia were identified, including sepsis, intra-abdominal infections, hospitalization within the last three months, prolonged antibiotic use (>1 week), <i>Candida</i> colonization, percutaneous endoscopic gastrostomy, central venous catheter, and acute kidney injury ( <i>p<</i>0.05). During the prospective phase, which evaluated 75 ICU patients, a Cerrahpaşa <i>Candida</i> score cutoff value of ≥4 points was established as significantly associated with an increased risk of candidemia (<i>p</i><0.05). The Cerrahpaşa <i>Candida</i> score and PCR assay, targeting the internal transcribed spacer (ITS) and D1/D2 regions, showed clinical utility for early diagnosis.</p><p><strong>Conclusion: </strong>The Cerrahpaşa <i>Candida</i> score and real-time PCR test developed in this study offer promising tools for guiding antifungal therapy in ICU patients, potentially reducing unnecessary antifungal use. However, improvements in PCR sensitivity are necessary. Further multicenter studies with larger patient cohorts are recommended to validate the clinical effectiveness of these diagnostic tools.</p>","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"306-319"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chikungunya Surge in Pakistan: A Call for Rapid Public Health Measures.","authors":"Marium Amjad","doi":"10.36519/idcm.2024.494","DOIUrl":"10.36519/idcm.2024.494","url":null,"abstract":"","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"349-350"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Community-Acquired Human Bocavirus Infection in an Immunocompetent Adult.","authors":"Oğuz Usta, Sidar Çöpür, Evren Şentürk, Çetin Atasoy, Önder Ergönül","doi":"10.36519/idcm.2024.372","DOIUrl":"10.36519/idcm.2024.372","url":null,"abstract":"<p><p>Human bocavirus 1 (HBoV1) is a recognized pathogen in respiratory infections among children; however, its prevalence and clinical implications in immunocompetent adults are unclear. We present a case of HBoV1 infection in a 61-year-old immunocompetent female patient with myositis, leading to respiratory failure. The involvement of respiratory muscles rather than lung parenchyma was observed. By this case, we highlight the importance of HBoV1 infection as a potential cause of viral pneumonia and myositis in adults. Prompt recognition and management of such cases are crucial for optimal patient outcomes and to avoid unnecessary use of antibiotics.</p>","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"339-342"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Adult-Onset Chronic Granulomatous Disease Case with Hemophagocytic Lymphohistiocytosis Caused by <i>Burkholderia</i> and <i>Aspergillus</i> Infections.","authors":"Yunus Kaygusuz, Ceren Üstün, Ahmet Görkem Er, Zehranur Kiki, İsmail Yaz, Sevda Tüten-Dal, Ayşegül Üner, Deniz Çağdaş, Ahmet Çağkan İnkaya, Murat Akova","doi":"10.36519/idcm.2024.381","DOIUrl":"10.36519/idcm.2024.381","url":null,"abstract":"<p><p>Chronic granulomatous disease (CGD) is a congenital disorder impairing phagocyte function, causing recurrent, life-threatening infections, and is rarely seen in adulthood. We present a 36-year-old male initially diagnosed with pneumonia. Bronchoalveolar lavage and blood cultures yielded <i>Burkholderia multivorans/cepacia</i> complex, sputum cultures <i>Aspergillus niger</i>. Despite the antimicrobial treatment, his condition deteriorated. His clinical and laboratory findings indicated hemophagocytic lymphohistiocytosis. He responded to steroids. Nitroblue tetrazolium and dihydroergotamine-123 tests confirmed CGD. Whole exome sequencing identified <i>NCF1</i> deletion. He received interferon-gamma, voriconazole, and trimethoprim-sulfamethoxazole. Allogeneic hematopoietic stem cell transplantation was planned. This case report improves understanding of CGD in adults, aiming to enhance diagnostic and therapeutic strategies.</p>","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"334-338"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Antibiotic Resistance Rates of <i>Mycobacterium tuberculosis</i> complex in Mycolor TK^® System.","authors":"Dilara Yıldırım, Özlem Aldemir, Tuğba Yanık-Yalçın, Mesut Parlak","doi":"10.36519/idcm.2024.435","DOIUrl":"10.36519/idcm.2024.435","url":null,"abstract":"<p><strong>Objective: </strong>Tuberculosis (TB) is a public health problem. This study aimed to determine the growth rates and drug susceptibility levels of patients with <i>Mycobacterium tuberculosis</i> complex (MTC) growth in cultures obtained and to compare the results with the growth rates and drug susceptibility levels found in our country and other countries. It also aimed to evaluate the results of supplementing classical methods such as Lowenstein-Jensen (LJ) with liquid TK MEDIUM^® and to determine the relationship between the growth rates obtained with both methods.</p><p><strong>Materials and methods: </strong>The study included patients who were Erlich Ziehl Neelsen (EZN) positive or negative and whose samples showed MTC growth in culture and susceptibility to the main drugs used in the treatment of TB, including streptomycin (SM), isoniazid (INH), rifampicin (RIF) and ethambutol (EMB). Patient samples were inoculated simultaneously into liquid TK^® MEDIUM based on color change and LJ solid medium. TB identification and antimicrobial susceptibility testing were performed using the TK ANTI TB and PNB KIT^® for the Mycolor TK^® automated mycobacterial culture system.</p><p><strong>Results: </strong>The study found resistance to dual drugs at 5.4%, INH+RIF at 1.35%, INH at 6.8%, RIF at 14.8%, EMB at 17.6%, and SM at 1.35%. Out of 69 samples cultured in LJ medium, three failed to grow in liquid TK^® MEDIUM, and two of 71 samples cultured in liquid TK^® MEDIUM gave negative results in solid LJ medium.</p><p><strong>Conclusion: </strong>We found that the drug resistance rates we obtained in our study were similar to the results of the Republic of Türkiye Ministry of Health and other studies conducted in our country. In a joint study conducted with liquid and solid media, no difference was found in the detection of TB bacilli. Mycolor TK^® automated system can enable rapid mycobacterial culture in laboratories with limited resources.</p>","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"320-327"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive Role and Clinical Value of Serum Cytokines in COVID-19.","authors":"Fatihan Pınarlık, Gizem Babuççu, Özgür Albayrak, Özlem Doğan, Şiran Keske, Yeşim Beşli, Füsun Can, Önder Ergönül","doi":"10.36519/idcm.2024.367","DOIUrl":"10.36519/idcm.2024.367","url":null,"abstract":"<p><strong>Objective: </strong><b>Cytokines and chemokines</b> are clinically relevant for severity prediction and treatment of COVID-19 caused by SARS-CoV-2. We aimed to demonstrate the potential cytokines for severity prediction in the five days after symptom onset and describe the importance of serum cytokine levels for patients with different disease severity.</p><p><strong>Materials and methods: </strong>Hospitalized COVID-19 patients and healthy control participants were recruited, and serial sera were collected from COVID-19 patients. Thirteen cytokines, including interleukin (IL) 1β, interferon (IFN) α2, IFN- γ, tumor necrosis factor (TNF) α, monocyte chemoattractant protein (MCP-1/CCL2), IL-6, IL-8 (CXCL8), IL-10, IL-12p70, IL-17A, IL-18, IL-23, and IL-33, were studied by bead-based multiplex assay by flow cytometry. Data regarding routine laboratory test results (leucocyte count, neutrophil count, lymphocyte count, platelet count, hemoglobin, liver transaminases, C-reactive protein [CRP], procalcitonin, and creatinine) were collected.</p><p><strong>Results: </strong>We demonstrated that COVID-19 patients had elevated serum levels of IFN-α2, TNF-α, MCP-1/CCL2, IL-6, IL-8, IL-18, IL-33 compared to healthy participants. Elevated levels of CRP and decreased lymphocyte count were observed in the critical disease group. Longitudinal analysis revealed a statistically significant increase in IL-6, IL-18, and MCP-1 serum levels of critical patients compared to healthy controls.</p><p><strong>Conclusion: </strong>MCP-1, IL-6, and IL-18 were found to be the best predictors of critical COVID-19 disease, and MCP-1 has the highest level of predictive performance.</p>","PeriodicalId":519881,"journal":{"name":"Infectious diseases & clinical microbiology","volume":"6 4","pages":"258-267"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}