Archiv EuroMedica最新文献

{"title":"MORPHOLOGICAL FEATURES OF DENTAL TISSUES IN STREPTOZOTOCIN-INDUCED DIABETES MELLITUS MODEL","authors":"D. Domenyuk, L. Ostrovskaya, O. Eremin, Denis Loginov, Yu.V. Kobzeva, Yuri Harutyunyan, S. Domenyuk","doi":"10.35630/2023/13/4.821","DOIUrl":"https://doi.org/10.35630/2023/13/4.821","url":null,"abstract":"The global issue of diabetes mellitus (DM), which is a serious burden faced by the healthcare systems and economies in many countries, has been increasing steadily in the recent decades. The fast pace of DM growth and prevalence, as well as associated complications (macro- and microangiopathy, neuropathy, nephropathy, impaired vision), high economic costs and social damage, high disability and mortality rates entailed by this disease take constant improvement of the respective diagnostics, prevention and treatment approaches. Dental manifestations of DM are diagnosed in most patients and reveal themselves through demineralized hard dental tissues, high intensity of dental caries and its prevalence, as well as the prevalence of periodontal diseases and issues affecting the oral mucosa. Our research included 60 male Wistar stock rats, all divided into two groups: the comparison group with no DM (n=10) and the main group with induced DM (n=50). DM modeling was done by a single intraperitoneal injection of streptozotocin (dosage – 65 mg/kg). The main group lab animals were removed from the experiment on Days 8, 16, 24, 32 and 60 under anesthesia by decapitation. All the histological and morphometric changes observed in the dental tissues were recorded in the ImadgeJ software package using a Leica DM2500 hardware and software set. The studied sections were used to evaluate the thickness of enamel, dentin, predentin, cement, pulp, the dentin tubule diameter, as well as the density of odontoblasts and ameloblasts. On Day 60 into the experiment, the following changes were identified in relation to the hard dental tissues: regarding the enamel − demineralization, disintegration of the interprism substance; deformed enamel prisms; erosion-type defects developing; regarding the dentin − expanded dentin tubules, vacuole dystrophy of odontoblasts, accumulating edematous fluid under the odontoblast layer, deformation and atrophy of odontoblasts; regarding the cement – layer thinning, cementolysis, cavities developing. Quantitative evaluation of rat dental tissues on Day 60, if compared with intact animals, revealed a multidirectional dynamics in the morphometric values. There was a statistically significant (p≤0.05) decrease in the enamel thickness identified (from 31.06±2.17 microns to 18.19±1.36 microns), in predentin (from 25.19±1.48 microns to 20.93±1.16 microns), in cement (from 37.28±2.04 microns to 31.04±1.46 microns), in the dentin tubule diameter (from 1.82±0.14 microns to 1.56±0.13 microns), as well as in the density of odontoblasts (from 7683.24±319.76 units/mm2 to 7312.61±256.19 units/mm2) along with a statistically reliable (p ≤0.05) increase in the thickness of dentin (from 96.54±3.39 microns to 105.11± 3.16 microns), pulp (from 107.43± 4.12 microns to 120.38±5.26 microns), as well as in the ameloblast density (from 6471.39± 272.18 units/mm2 to 6849.06±253.84 units/mm2). The outcomes expand the understanding of the structural changes affecting ra","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48141693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CELLULAR COMPOSITION OF THE LYMPHOID STRUCTURES OF THE HUMAN INTRAHEPATIC BILE DUCTS","authors":"Naile Aliyeva","doi":"10.35630/2023/13/4.808","DOIUrl":"https://doi.org/10.35630/2023/13/4.808","url":null,"abstract":"he aim of our study was to investigate the cellular composition of the lymphoid structures of the human intrahepatic bile ducts. Materials and Methods: The object of the study was the lymphoid structures of the walls of the intrahepatic bile ducts obtained from 45 cadavers of different ages. Lymphoid nodules in the walls of the intrahepatic bile ducts were investigated by T. Hellman’s method. The microanatomy of lymphoid structures was studied on microscopic preparations. Microscopic preparations were stained according to Van Gieson, with hematoxylin-eosin, methylene blue. The digital data obtained during the study was subjected to statistical processing. Results: A macromicroscopic study of lymphoid formations in the intrahepatic bile ducts showed that lymphoid formations in the walls of these organs are represented by lymphoid nodules and diffuse lymphoid tissue. The peripheral contours of the lymphoid nodules are clearly defined. Histologically, the cellular composition of lymphoid nodules and diffuse lymphoid tissue was studied. In the mucous membrane and in the submucosa, small, medium sized lymphocytes, and large lymphocytes, macrophages, plasma, reticular, mast cells, cells in a stage of mitosis, degeneratively altered (destructive), and other forms of lymphoid cells are determined. Conclusions: According to our data, newborns have not only lymphoid nodules, but also a typical cellular composition in the lymphoid tissue, which has a completely “mature character”. The results of morphometric studies showed that in early childhood there is a high percentage of small, medium, large lymphocytes, macrophages, cells with signs of mitosis.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42697737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RESULTS OF THE USE OF POLYVALENT BACTERIOPHAGES IN THE TREATMENT OF INFECTIOUS COMPLICATIONS IN EMERGENCY ABDOMINAL SURGERY","authors":"DMITRY PARSHIN, Vyacheslav Mykhaylichenko, A. Kupriyanov, Ramazan Alibekov, R. Mardanov, Enver Kеrimov, G. Puchkina, O. Bezrukov, A. Abdullaev","doi":"10.35630/2023/13/4.813","DOIUrl":"https://doi.org/10.35630/2023/13/4.813","url":null,"abstract":"We comparatively studied the results of treatment of 86 patients with surgical infectious complications after cavitary intra-abdominal operations for emergency abdominal diseases. In the main group, treatment was supplemented with polyvalent bacteriophages. We studied integral scales for assessing the severity of patient’s condition of (APACHE II, ACI, ASEPSIS), microbial pattern, enteral morphofunctional coefficient, levels of alkaline phosphatase and intestinal alkaline phosphatase, and indicators of cellular immunity. The results of the study show that complex treatment including polyvalent bacteriophages in postoperative purulent-inflammatory complications in urgent surgery reduces the development of antibiotic-resistant strains, as well as the risk of nosocomial microbial wound associations. Enteral application of polyvalent bacteriophages in patients with acute intestinal failure syndrome normalizes microbial enteral imbalance and the level of pathogens contamination of the small intestine. Supplementing the treatment of postoperative abdominal purulent-inflammatory complications with phage therapy can reduce the number of surgical interventions, as well as the treatment duration and mortality.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46012517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A CASE OF DEVELOPING SQUAMOUS CELL AND BASAL-CELL CARCINOMA IN PATIENTS WITH PSORIASIS","authors":"E. Efanova, Y. Rusak, E. Vasilieva","doi":"10.35630/2023/13/4.820","DOIUrl":"https://doi.org/10.35630/2023/13/4.820","url":null,"abstract":"The described clinical example illustrates the rare association of psoriasis with basal cell and squamous cell carcinoma. This clinical case may be of interest to dermatologists and oncologists. It illustrates the neglect of the process in view of the late treatment of the patient. The rising incidence rates of skin cancer are caused by exposure to UVB procedures and increasing with age, which emphasizes the importance of thorough dermatological screening among patients with psoriasis and histological examination of old psoriatic plaques. Alertness for the early detection of skin tumors is relevant not only among medical workers, but also among the population.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45239160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INFLUENCE OF CD68 POSITIVE CELLS ON THE PROCESSES OF PROLIFERATION AND APOPTOSIS IN THE STRUCTURES OF THE RESPIRATORY SYSTEM","authors":"A. Protsko, T. Shishkina, L. Naumova, A. Zhidovinov, Irina Chekunova, Irina S. Davlatova, O. Ovsyannikova","doi":"10.35630/2023/13/4.809","DOIUrl":"https://doi.org/10.35630/2023/13/4.809","url":null,"abstract":"Aim: To study the influence of macrophages or CD68 positive cells on the processes of proliferation and apoptosis in the respiratory system. Materials and Methods: Experimental modeling of chronic hypoxic hypoxia in combination with inhalation of natural gas was carried out on 133 outbred male rats. Hypoxia was simulated using special seed chambers and natural gas from the Astrakhanskoye Conventional Gas Field at a concentration of 3 mg/m3. The animals were placed under these conditions four hours a day for five days a week for four months. The animals were removed once a month - after 30, 60, 90 and 120 days. The activity of proliferation and apoptosis processes was studied by immunohistochemical method, followed by analysis of the expression of the proliferation marker Ki67, the apoptosis marker p53. The morphofunctional activity of macrophages was determined by the distribution of CD68 positive cells in structural components of respiratory system and the cytokine content in arterial blood taken from the abdominal aorta, that is, at the exit from lungs. Results: With the increase of the chronic experiment duration, the formation of two types of multidirectional morphological changes was revealed: thinning and stretching of the interalveolar septa, an increase in the volume of the alveoli in the peripheral parts of the lungs, and thickening of the interalveolar septa due to increased blood supply and edema-swelling in the early stages and proliferation of fibrous connective tissue in later periods; whereas the diameter and volume of the alveoli became smaller. Under chronic hypoxia combined with the action of natural gas, in all studied structures of the respiratory system there was an increase in the activity of CD68 positive cells. This is manifested by an increase in their number and activation of spontaneous and induced production of the pro-inflammatory cytokine TNFα. Besides, in areas of the lungs with changes in the type of atelectasis, an increase in the expression of the proliferation marker Ki67 and the apoptosis marker p53 was revealed. The latter is most likely associated with a deterioration in blood supply and oxygenation of these areas and the migration of leukocyte and fibroblast cells here, followed by their proliferation and formation pulmonary fibrosis. In areas with emphysematous changes, a decrease in the content of Ki67 positive cells was revealed with a simultaneous increase in the number of p53 positive cells. Conclusion: Thus, it is possible to determine a direct relationship between the increase in the morphofunctional activity of CD68 positive cells and the intensity of proliferation and apoptosis processes in the structural components of the respiratory system under chronic hypoxia combined with natural gas inhalation.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44759168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHALLENGES OF THE PANDEMIC AND WAR: ASPECTS OF HEALTH AND MENTAL WELL-BEING-2030","authors":"I. Khozhylo, L. Pavlenko, N. Lipovska, Katerina Sakharova","doi":"10.35630/2023/13/4.802","DOIUrl":"https://doi.org/10.35630/2023/13/4.802","url":null,"abstract":"The article deals with challenges, caused by the COVID-19 pandemic and Russia’s full-scale war against Ukraine. Aspects of health and mental well-being 2030 are in the focus of the research. The major challenges in the healthcare systems through the prism of their resilience and emergence are examined. The study emphasises that the achievement of the Sustainable Development Goals (SDGs) in the healthcare sector is possible through the application of the principle of universal access to healthcare services. This approach ensures the integration of Goal 3 \"Good Health and Well-being\" with other SDGs, namely: poverty eradication (SDG 1), economic growth and decent work for all (SDG 8), achieving inclusive and equitable education (SDG 4), equality and empowers women and girls (SDG 5), peace, justice and building strong institutions at the national and global levels (SDG 16). The Polish experience in organizing anti-epidemic measures to combat COVID-19 is described as a useful example for Ukraine because of the similarity of the initial conditions for the functioning of the healthcare industry at the national level. Considerable attention is paid to the healthcare system of Ukraine during the war, in particular, aspects of public administration. The data presented in the article are the legal acts of the UN and WHO and legal acts of the countries of the European region (Ukraine, Poland), which are freely available on the Internet.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46657919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANALYSIS OF DIELECTRIC PROPERTIES OF HUMAN TISSUES: RESULTS OF EX VIVO EXPERIMENTS","authors":"A. Martusevich, V. Nazarov, A. Surovegina, Aleksandra Fedotova, Y. Kiseliv, Pavel Suldin, Sergey Petrov","doi":"10.35630/2023/13/3.306","DOIUrl":"https://doi.org/10.35630/2023/13/3.306","url":null,"abstract":"The aim of the work was to evaluate the dielectric properties of human tissues in ex vivo conditions. The study was performed on 68 tissue samples, 20 of which were fragments of muscle tissue, 17 – fatty, 11 – fibrous and 20 – a full-layer skin flap. The analysis of the dielectric properties of tissue fragments was carried out by the method of near-field resonant microwave sounding using a special software and hardware complex. The study made it possible to verify the presence of pronounced differences in the dielectric properties of tissues depending on their histotype. It was revealed that adipose tissue has the highest level of dielectric permittivity, and fibrous tissue has the lowest one. Muscle tissue and a full-layered skin flap occupy an intermediate position between them. These data are fundamentally important for the creation of standards for the dielectric characteristics of biological objects and the further development of near-field resonant microwave sensing technology as an innovative medical imaging technology.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42880358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NEUROENDOCRINE AND IMMUNE INTERACTION IN AUTOIMMUNE THYROIDITIS","authors":"R. Rahimova, Gulnara Dashdamirova, I. Shahverdiyeva, V. Yagubova, N. Bayramova","doi":"10.35630/2023/13/3.309","DOIUrl":"https://doi.org/10.35630/2023/13/3.309","url":null,"abstract":"The purpose of the study is to elucidate the interrelationships and notable correlations among the indicators of hormonal regulation, immune status, and markers of neurodegeneration in patients diagnosed with autoimmune thyroiditis. The study enrolled a total of 170 patients with autoimmune thyroiditis (divided subclinical and manifest form). Control group consist of 65 people without thyroid pathologies or other autoimmune diseases. The examination of cytokine profiles in patients with autoimmune thyroiditis revealed a significant increase in cytokine levels in groups of patients with subclinical and manifest forms of the disease compared to the control group. The analysis of antibody levels in patients with different clinical forms of hypothyroidism revealed that the median concentration of Ab-nDNA was significantly higher in patients with a manifest form of the disease compared to those with a subclinical group. According to an assessment of neuron-spesific enolase levels in the blood plasma of patients with autoimmune thyroiditis significantly compared to control group. Furthermore, a study examining the concentration of neuron-spesific enolase in patients with different clinical forms of autoimmune thyroiditis revealed that individuals with a subclinical form of the disease had statistically significant increase (p=0.042) in the value of this parameter. Our study results revealed significant changes in immunological parameters and markers of neurodegeneration in patients with autoimmune thyroiditis alongside hormonal imbalance.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48455819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORPHOMETRIC CHARACTERISTICS OF THE MANDIBULAR CONDYLAR PROCESSES","authors":"H. Darawsheh, S. Dydykin, Aisr Zmiro, S. Gupalo, M. Kapitonova, G. Pakhlevanyan, E. Terekhina, N. Tiunova, Viktoria Vladimirova, Emin Mammadov, Yuri Vasil'ev","doi":"10.35630/2023/13/3.305","DOIUrl":"https://doi.org/10.35630/2023/13/3.305","url":null,"abstract":"Aim: to study the morphometric characteristics of the mandibular condylar processes to identify patterns and their classification. Materials and Methods: a study of 49 skeletonized and certified mandibular preparations was performed using a caliper and a depth gauge. During the study of the material, the forms and the main morphometric parameters of the condylar processes were determined and analyzed. Statistical data processing was carried out using the Microsoft Excel program. Results: The study of certified biological material yielded the main morphometric and morphofunctional parameters of the mandibular condylar processes. We revealed characteristic differences in the anatomical structures of the studied preparations. The main variants and types of combinations of forms of condylar processes were singled out: oval, hook-shaped, diamond-shaped, L-shaped. The lowest asymmetry index is characteristic of a combination of oval and diamond–shaped forms - 2.44%., and the highest asymmetry index is characteristic of diamond–shaped and L-shaped forms of condylar processes - 7.13%. Conclusion: The presented data can help practitioners to more accurately determine the target point for Gow-Gates anesthesia; to take into account the nature of the influence of structural changes in the joint on other elements of the dental apparatus, thus preventing functional diseases of the temporomandibular joint. The methodology of studying the main morphometric parameters on skeletonized preparations of the lower jaws can be adapted for the examination of living persons using diagnostic radiology.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42656513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FEATURES OF THE DEVELOPMENT AND HEALTH CONDITION OF CHILDREN CONCEIVED BY IN VITRO FERTILIZATION","authors":"Nailya R. Pimenova, E. Kashirskaya, N.P. Provatar, E. Novikova, N. Tkacheva, Aleksey Zhidovinov, L. Goncharova","doi":"10.35630/2023/13/3.310","DOIUrl":"https://doi.org/10.35630/2023/13/3.310","url":null,"abstract":"Aim: To study the anamnesis, development parameters and health condition in the first year of life of the children conceived by IVF. Materials and Methods: 116 children born by IVF and 46 children born in natural pregnancy were examined. Results: In most cases of our study, children after IVF were born prematurely from women with a bad obstetric and gynecological history and had a low birth weight. The period of early adaptation in premature infants born after assisted reproductive technologies was complicated by respiratory distress syndrome. Most often the children after IVF had such pathologies as central nervous system damage, deficiency anemia and minor anomalies of heart development in the neonatal period. Compared with children born in natural pregnancy, infectious diseases were more often detected in newborns after IVF. The development of bronchopulmonary dysplasia was significantly more frequent in children after IVF within a month of life. At six months, most of the IVF babies had low parameters of physical development, whereas weight and growth indicators did not differ significantly from the general population indicators by the first year of life. Most of the children born after IVF had neurological outcomes, such as delayed psychomotor development, malfunctioning of the autonomic nervous system, convulsive syndrome by the year of life. Conclusion: Children born after IVF have features of somatic status, neuropsychiatric and physical development, which indicates the need for targeted monitoring and continued research in this group of children to minimize health risks.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45974064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}