Frontiers in insect science最新文献

{"title":"Molecular characterization, localization, and physiological roles of ITP and ITP-L in the mosquito, <i>Aedes aegypti</i>.","authors":"Farwa Sajadi, Jean-Paul V Paluzzi","doi":"10.3389/finsc.2024.1374325","DOIUrl":"10.3389/finsc.2024.1374325","url":null,"abstract":"<p><p>The insect ion transport peptide (ITP) and its alternatively spliced variant, ITP-like peptide (ITP-L), belong to the crustacean hyperglycemic hormone family of peptides and are widely conserved among insect species. While limited, studies have characterized the ITP/ITP-L signaling system within insects, and putative functions including regulation of ion and fluid transport, ovarian maturation, and thirst/excretion have been proposed. Herein, we aimed to molecularly investigate <i>Itp</i> and <i>Itp-l</i> expression profiles in the mosquito, <i>Aedes aegypti</i>, examine peptide immunolocalization and distribution within the adult central nervous system, and elucidate physiological roles for these neuropeptides. Transcript expression profiles of both <i>AedaeItp</i> and <i>AedaeItp-l</i> revealed distinct enrichment patterns in adults, with <i>AedaeItp</i> expressed in the brain and <i>AedaeItp-l</i> expression predominantly within the abdominal ganglia. Immunohistochemical analysis within the central nervous system revealed expression of <i>Aedae</i>ITP peptide in a number of cells in the brain and in the terminal ganglion. Comparatively, <i>Aedae</i>ITP-L peptide was localized solely within the pre-terminal abdominal ganglia of the central nervous system. Interestingly, prolonged desiccation stress caused upregulation of <i>AedaeItp</i> and <i>AedaeItp-l</i> levels in adult mosquitoes, suggesting possible functional roles in water conservation and feeding-related activities. RNAi-mediated knockdown of <i>AedaeItp</i> caused an increase in urine excretion, while knockdown of both <i>AedaeItp</i> and <i>AedaeItp-l</i> reduced blood feeding and egg-laying in females as well as hindered egg viability, suggesting roles in reproductive physiology and behavior. Altogether, this study identifies <i>Aedae</i>ITP and <i>Aedae</i>ITP-L as key pleiotropic hormones, regulating various critical physiological processes in the disease vector, <i>A. aegypti</i>.</p>","PeriodicalId":517424,"journal":{"name":"Frontiers in insect science","volume":"4 ","pages":"1374325"},"PeriodicalIF":2.4,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11035804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conservation of <i>shibire</i> and <i>RpII215</i> temperature-sensitive lethal mutations between <i>Drosophila</i> and <i>Bactrocera tryoni</i>.","authors":"Thu N M Nguyen, Amanda Choo, Simon W Baxter","doi":"10.3389/finsc.2024.1249103","DOIUrl":"10.3389/finsc.2024.1249103","url":null,"abstract":"<p><p>The sterile insect technique can suppress and eliminate population outbreaks of the Australian horticultural pest, <i>Bactrocera tryoni</i>, the Queensland fruit fly. Sterile males mate with wild females that produce inviable embryos, causing population suppression or elimination. Current sterile insect releases are mixed sex, as the efficient removal of unrequired factory-reared females is not yet possible. In this paper, we assessed the known <i>Drosophila melanogaster</i> temperature-sensitive embryonic lethal alleles <i>shibire</i> (G268D, <i>shi^ts1</i>) and <i>RNA polymerase II 215</i> (R977C, <i>RpII215^ts</i>) for potential use in developing <i>B. tryoni</i> genetic sexing strains (GSS) for the conditional removal of females. Complementation tests in <i>D. melanogaster</i> wild-type or temperature-sensitive genetic backgrounds were performed using the GAL4-UAS transgene expression system. A <i>B. tryoni</i> wild-type <i>shibire</i> isoform partially rescued <i>Drosophila</i> temperature lethality at 29°C by improving survivorship to pupation, while expressing <i>B. tryoni shi^ts1</i> failed to rescue the lethality, supporting a temperature-sensitive phenotype. Expression of the <i>B. tryoni RpII215</i> wild-type protein rescued the lethality of <i>D. melanogaster RpII215^ts</i> flies at 29°C. Overexpressing the <i>B. tryoni RpII215^ts</i> allele in the <i>D. melanogaster</i> wild-type background unexpectedly produced a dominant lethal phenotype at 29°C. The <i>B. tryoni shibire</i> and <i>RpII215</i> wild-type alleles were able to compensate, to varying degrees, for the function of the <i>D. melanogaster</i> temperature-sensitive proteins, supporting functional conservation across species. <i>Shibire</i> and <i>RpII215</i> hold potential for developing insect strains that can selectively kill using elevated temperatures; however, alleles with milder effects than <i>shi^ts1</i> will need to be considered.</p>","PeriodicalId":517424,"journal":{"name":"Frontiers in insect science","volume":"4 ","pages":"1249103"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10926519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Bt corn expressing <i>Bacillus thuringiensis</i> Berliner insecticidal proteins on the growth and survival of <i>Spodoptera frugiperda</i> larvae in Colombia.","authors":"Jairo Rodriguez-Chalarca, Sandra J Valencia, Alejandra Rivas-Cano, Francisco Santos-González, Diana Patricia Romero","doi":"10.3389/finsc.2024.1268092","DOIUrl":"10.3389/finsc.2024.1268092","url":null,"abstract":"<p><p>Bioassays were conducted under controlled conditions to determine the response of <i>Spodoptera frugiperda</i> (J. E. Smith) larvae fed with corn materials expressing <i>Bacillus thuringiensis</i> (Bt) insecticidal endotoxins: (1) VT Double Pro^® (VT2P) expressing Cry1A.105-Cry2Ab2 proteins and (2) VT Triple Pro^® (VT3P) expressing Cry1A.105-Cry2Ab2-Cry3Bb1 proteins. The parameters assessed were: (i) mortality rate, and (ii) growth inhibition (GI) with respect to the control. To conduct this study, larvae were collected from commercial non-Bt corn fields, in four agricultural sub-regions in Colombia, between 2018 and 2020. Fifty-two populations were assessed from the field and neonate larvae from each of the populations were used for the bioassays. The study found that mortality rates in the regions for larvae fed with VT2P corn ranged from 95.1 to 100.0%, with a growth inhibition (%GI) higher than 76.0%. Similarly, mortality rate for larvae fed with VT3P corn were between 91.4 and 100.0%, with a %GI above 74.0%. The population collected in Agua Blanca (Espinal, Tolima; Colombia) in 2020, showed the lowest mortality rate of 53.2% and a %GI of 73.5%, with respect to the control. The population that exhibited the lowest %GI was collected in 2018 in Agua Blanca (Espinal, Tolima, Colombia) with a 30.2%, growth inhibition, with respect to the control. In recent years, the use of plant tissue to monitor susceptibility to fall armyworm has proven to be useful in the resistance management program for corn in Colombia determining that the FAW populations are still susceptible to Bt proteins contained in VT2P and VT3P.</p>","PeriodicalId":517424,"journal":{"name":"Frontiers in insect science","volume":"4 ","pages":"1268092"},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10926427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate identification of <i>Helicoverpa armigera-Helicoverpa zea</i> hybrids using genome admixture analysis: implications for genomic surveillance.","authors":"Dario Trujillo, Thiago Mastrangelo, Consuelo Estevez de Jensen, Jose Carlos Verle Rodrigues, Roger Lawrie, Steven E Massey","doi":"10.3389/finsc.2024.1339143","DOIUrl":"10.3389/finsc.2024.1339143","url":null,"abstract":"<p><p><i>Helicoverpa armigera</i>, the cotton bollworm moth, is one of the world's most important crop pests, and is spreading throughout the New World from its original range in the Old World. In Brazil, invasive <i>H. armigera</i> has been reported to hybridize with local populations of <i>Helicoverpa zea</i>. The correct identification of <i>H. armigera-H. zea</i> hybrids is important in understanding the origin, spread and future outlook for New World regions that are affected by outbreaks, given that hybridization can potentially facilitate <i>H. zea</i> pesticide resistance and host plant range via introgression of <i>H. armigera</i> genes. Here, we present a genome admixture analysis of high quality genome sequences generated from two <i>H. armigera-H. zea</i> F1 hybrids generated in two different labs. Our admixture pipeline predicts 48.8% and 48.9% <i>H. armigera</i> for the two F1 hybrids, confirming its accuracy. Genome sequences from five <i>H. zea</i> and one <i>H. armigera</i> that were generated as part of the study show no evidence of hybridization. Interestingly, we show that four <i>H. zea</i> genomes generated from a previous study are predicted to possess a proportion of <i>H. armigera</i> genetic material. Using unsupervised clustering to identify non-hybridized <i>H. armigera</i> and <i>H. zea</i> genomes, 8511 ancestry informative markers (AIMs) were identified. Their relative frequencies are consistent with a minor <i>H. armigera</i> component in the four genomes, however its origin remains to be established. We show that the size and quality of genomic reference datasets are critical for accurate hybridization prediction. Consequently, we discuss potential pitfalls in genome admixture analysis of <i>H. armigera-H. zea</i> hybrids, and suggest measures that will improve such analyses.</p>","PeriodicalId":517424,"journal":{"name":"Frontiers in insect science","volume":"4 ","pages":"1339143"},"PeriodicalIF":2.4,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10926370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Dichotomous sperm in Lepidopteran insects: a biorational target for pest management.","authors":"Rakesh K Seth, Priya Yadav, Stuart E Reynolds","doi":"10.3389/finsc.2024.1343089","DOIUrl":"https://doi.org/10.3389/finsc.2024.1343089","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/finsc.2023.1198252.].</p>","PeriodicalId":517424,"journal":{"name":"Frontiers in insect science","volume":"4 ","pages":"1343089"},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10926491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Current status of honey bee genetic and breeding programs: progress and perspectives.","authors":"María Alejandra Palacio, Ernesto Guzman-Novoa, Alejandra Carla Scannapieco, Agostina Giacobino, Fanny Mondet","doi":"10.3389/finsc.2024.1365665","DOIUrl":"https://doi.org/10.3389/finsc.2024.1365665","url":null,"abstract":"","PeriodicalId":517424,"journal":{"name":"Frontiers in insect science","volume":"4 ","pages":"1365665"},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10926512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Modeling-informed Engineered Genetic Incompatibility strategies to overcome resistance in the invasive <i>Drosophila suzukii</i>.","authors":"Adam Sychla, Nathan R Feltman, William D Hutchison, Michael J Smanski","doi":"10.3389/finsc.2023.1360167","DOIUrl":"https://doi.org/10.3389/finsc.2023.1360167","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/finsc.2022.1063789.].</p>","PeriodicalId":517424,"journal":{"name":"Frontiers in insect science","volume":"3 ","pages":"1360167"},"PeriodicalIF":0.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10926383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}