Narra J最新文献

{"title":"Genetic variations of the <i>L2</i> gene in human papillomavirus (HPV) type 16 from cervical cancer patients in Sumatra region, Indonesia.","authors":"Maya Savira, Analdi Farniga, Zidny Ilmiah, Rachmadina Rachmadina, Ika A Rini, Rahmat A Kemal, Azza E Mahargyarani, Muhammad Z Admiral, Amru Sofian, Renardy R Razali, Donel Suhaimi, Andani E Putra","doi":"10.52225/narra.v5i2.1653","DOIUrl":"10.52225/narra.v5i2.1653","url":null,"abstract":"<p><p>The L2 protein, a minor capsid component of human papillomavirus (HPV), plays a critical role in the HPV life cycle by packaging the viral genome with the L1 protein and facilitating DNA transport to the nucleus. Identifying genetic variations in the <i>L2</i> gene is essential for improving vaccine development, diagnostic accuracy, and understanding viral evolution, potentially contributing to more effective HPV vaccines. The aim of this study was to investigate the genetic variation of the <i>L2</i> gene in cervical cancer specimens collected from patients in Riau Province, Indonesia. A single-center, cross-sectional study was conducted at Arifin Achmad General Hospital, Riau Province, involving cervical cancer patients with confirmed HPV16 infection between January 2018 and August 2020. Demographic, clinical, and risk factor data were collected through structured interviews and direct assessments. Cervical biopsy specimens were collected, and viral DNA was extracted for <i>L2</i> gene amplification using polymerase chain reaction (PCR). Sequencing was conducted on PCR products, followed by single-nucleotide polymorphism (SNP) identification through alignment with the HPV16 reference genome. The amplification and sequencing of the HPV16 <i>L2</i> gene from 22 cervical cancer specimens revealed 36 SNPs, including 31 nonsynonymous and five synonymous mutations. High-frequency mutations were observed at nucleotide positions 4,074 and 4,177, each detected in 95.45% of the samples. Notable insertions were found at positions 3,668-3,669 and 4,275-4,276, indicating substantial sequence variation. Phylogenetic analysis grouped the sequences into three clusters, with most belonging to sub-lineage A2 (European), while others aligned with A4 (Asian) and East Asian lineages. The observed genetic diversity in the HPV16 <i>L2</i> gene may reflect regional viral evolution and has potential implications for future vaccine development.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e1653"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of anthropometric adiposity and excessive daytime sleepiness on endothelial function in healthcare workers: A cross-sectional analysis.","authors":"Vito A Damay, Ignatius Ivan, Nadhira A Islami, Kenza Y Rubismo","doi":"10.52225/narra.v5i2.2003","DOIUrl":"10.52225/narra.v5i2.2003","url":null,"abstract":"<p><p>Obesity and excessive daytime sleepiness (EDS) are known contributors to cardiovascular risk through their impact on endothelial function. Healthcare workers, frequently exposed to shift work, are particularly vulnerable to these risk factors. The aim of this study was to assess the relationship between anthropometric adiposity measures and EDS with endothelial function, measured via flow-mediated dilation (FMD), in healthcare workers. This cross-sectional study included 82 healthcare workers aged 20-50 years without pre-existing cardiovascular conditions. Anthropometric measures such as body mass index (BMI), waist circumference (WC), and waist-to-height ratio (WHtR) were collected to assess adiposity. EDS was evaluated using the Epworth sleepiness scale (ESS), with a score ≥10 indicating EDS. Endothelial function was measured via FMD, with values <7.1% indicating dysfunction. Multivariate logistic regression was used to identify predictors of endothelial dysfunction, adjusting for confounders such as age and sex. Collinearity diagnostics, including the Belsley-Kuh-Welsch method, were applied to confirm multicollinearity and refine the regression model. Overweight and obesity, high-risk WC, and increased risk WHtR were associated with endothelial dysfunction (<i>p</i><0.001), with WHtR showing an independent association (adjusted odds ratio (AOR): 8.48; 95%CI: 2.58-27.86; <i>p</i><0.001). EDS also showed a significant independent association with impaired FMD outcomes (AOR: 3.73; 95%CI: 1.23-11.26; <i>p</i>=0.020). Pearson correlation analysis revealed significant negative correlations between BMI (<i>r</i>=-0.483, <i>p</i><0.001), WC (<i>r</i>=-0.473, <i>p</i><0.001), and WHtR (<i>r</i>=-0.432, <i>p</i><0.001) with FMD, indicating that higher adiposity levels were linked to poorer endothelial function. Obesity and poor sleep quality, even in the absence of cardiovascular disease, are associated with an increased risk of endothelial dysfunction in healthcare workers. Early intervention focusing on weight management and improving sleep quality could mitigate future cardiovascular risks in this population.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e2003"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the hypoglycemic potential of fresh, <i>semangit</i>, and <i>bosok</i> tempe: A comparative metabolite profile.","authors":"Made Astawan, Zaid Abdurrasyid, Rias R Novita, Aprilia F Damayanti, Saraswati Saraswati, Tutik Wresdiyati, Pramuan Saithong, Wanida T Chitisankul, Sastia P Putri","doi":"10.52225/narra.v5i2.2327","DOIUrl":"10.52225/narra.v5i2.2327","url":null,"abstract":"<p><p>Tempe, a traditional Indonesian fermented soybean product made with <i>Rhizopus</i> spp., is classified based on fermentation duration into fresh (two days), <i>semangit</i> (five days), and <i>bosok</i> (seven days) varieties, fermented at room temperature (28-30°C). Longer fermentation is believed to enhance its antidiabetic properties. The aim of this study was to compare the metabolite profiles and hypoglycemic activities of fresh, <i>semangit</i>, and <i>bosok</i> tempe made from germinated and non-germinated soybeans. Diabetic rat models were used to assess the effects of these tempe types on body weight, blood glucose levels, serum insulin, pancreatic β-cell count, and glycogen content in liver and muscle tissues. Metabolomic profiling was conducted using gas chromatography-mass spectrometry (GC-MS), followed by principal component analysis (PCA) to assess the influence of fermentation stage and germination. Fresh tempe, especially from germinated soybeans, had the highest moisture content. Fermentation duration significantly influenced color, texture, and pH, with <i>bosok</i> tempe showing the most notable changes. Tempe and gliclazide significantly reduced blood glucose in diabetic rats in vivo, with <i>semangit</i> and <i>bosok</i> tempe restoring levels close to normal. However, weight loss was not reversed. <i>Bosok</i> non-germinated tempe induced the highest insulin levels among tempe treatments and improved β-cell count and density to levels comparable with gliclazide. Glycogen stores in the liver and muscle were significantly restored by tempe, with <i>bosok</i> non-germinated tempe showing the greatest effect. GC-MS profiling identified 154 metabolites, of which 63 were annotated. Fermentation and germination shifted the metabolite profile, with <i>bosok</i> non-germinated tempe showing the highest diversity, including amino acids, sugars, and amines. PCA separated samples by fermentation stage, highlighting metabolite accumulation with prolonged fermentation. The findings revealed that <i>bosok</i> tempe from non-germinated soybeans had the highest abundance of bioactive metabolites, including isoflavones, which likely contributed to its superior antioxidant and hypoglycemic potential compared to other tempe types.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e2327"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential effects of <i>Lactococcus lactis</i> D4 on the proliferation, apoptosis, and inflammatory responses in colorectal cancer cells.","authors":"Muhammad I Rivai, Ronald E Lusikooy, Andani E Putra, Aisyah Elliyanti, Ade Sukma","doi":"10.52225/narra.v5i2.1596","DOIUrl":"10.52225/narra.v5i2.1596","url":null,"abstract":"<p><p><i>Lactococcus lactis</i> D4 is a probiotic produced through the fermentation of buffalo milk in bamboo, namely \"<i>dadih</i>\", a traditional food from West Sumatera, Indonesia. To the best of our knowledge, no specific research has investigated the effects of <i>L. lactis</i> D4, derived from <i>dadih</i> extraction, on colorectal cancer or its potential clinical applications. Therefore, the aim of this study was to evaluate the potential of <i>L. lactis</i> D4 from <i>dadih</i> to inhibit colorectal cancer growth in rat models, with a focus on its effects on cell proliferation, apoptosis, and inflammatory responses. An in vivo study was conducted using 37 male Sprague-Dawley rats, allocated into five groups: (1) control (no treatment), (2) dysplasia (induced with 1,2-dimethylhydrazine until dysplasia developed), (3) dysplasia + <i>L. lactis</i> D4 (induced with 1,2-dimethylhydrazine, then treated with <i>L. lactis</i> D4 after dysplasia confirmation), (4) cancer (induced with 1,2-dimethylhydrazine until cancer was confirmed), and (5) cancer + <i>L. lactis</i> D4 (induced with 1,2-dimethylhydrazine until cancer was confirmed, then treated with <i>L. lactis</i> D4 for 15 days). The effects of <i>L. lactis</i> D4 on cancer progression were assessed through immunohistochemical analysis of cell proliferation (cyclin D1, Bcl-2), apoptosis (p53, caspase-3), and inflammation (nuclear factor-κB (NF-κB) and cyclooxygenase-2 (COX-2)). This study found that <i>L. lactis</i> D4 treatment reduced adenocarcinoma and dysplasia severity in colorectal cancer models through significant reduction in cyclin D1, Bcl-2, NF-κB, and COX-2 expression observed across all groups (<i>p</i><0.01), although changes in dysplasia and cancer subgroups were not statistically significant (<i>p</i>>0.05). No statistically significant change was noted in p53 expression (<i>p</i>=0.518), whereas caspase-3 expression varied significantly across groups (<i>p</i>=0.010). In conclusion, <i>L. lactis</i> D4 reduces the expression of cyclin D1, Bcl-2, NF-κB, and COX-2 proteins, offering insights into its potential to modulating proliferation and inflammation in colorectal cancer growth.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e1596"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal ingestion rate based on worker activity patterns during stool handling in a ruminant farm.","authors":"Yudith V Paramitadevi, Cindy R Priadi, Iftita Rahmatika, Andriansjah Rukmana","doi":"10.52225/narra.v5i2.1989","DOIUrl":"10.52225/narra.v5i2.1989","url":null,"abstract":"<p><p>Farm workers who handle livestock stools face an increased risk of infection by pathogenic bacteria, such as <i>Escherichia coli</i> O157 and <i>Salmonella spp</i>., leading to millions of severe health issues and thousands of fatalities annually. The aim of this study was to assess the impact of these pathogens by measuring their concentrations, determining rates of unintentional fecal consumption, and conducting a quantitative assessment of microbial risk. An integrated farm in Sukabumi City, Indonesia, was examined for <i>E. coli</i> O157 and <i>Salmonella spp</i>. in livestock stools. Additionally, the study monitored the rate of incidental fecal ingestion among farm workers. Stool samples were collected (n= 40) from ruminants and analyzed following ISO 16649-1:2018, ISO 9308-1:2014, and ISO 6579-1:2017/Amd.1:2020. The study tracked worker's behavior daily to determine the contact time. The fecal ingestion rate was calculated by multiplying the estimated stool weight ingested by the contact time workers spent cleaning livestock stools in the barn each day. Microbial analysis revealed that the highest concentration of <i>E. coli</i> O157 in beef cattle stools was 2.49 log₁₀ CFU/g. The study determined mean fecal ingestion rates during the dry season (8.64 mg/day) and rainy season (6.84 mg/day). Results from the quantitative microbial risk assessment showed that stool from beef cattle posed a higher risk of <i>E. coli</i> O157 infection compared to other ruminants, with an estimated disease burden of 9.8 × 10^-3 pppy. This study represents the first comprehensive quantitative evaluation of fecal ingestion by farm workers during animal husbandry. The findings underscore the need for improved worker safety measures, such as enhanced sanitation practices and protective equipment, to mitigate the risks of handling livestock stools.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e1989"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key contents of health education and their impact on improving medication adherence among hypertensive patients: A systematic review and meta-analysis.","authors":"Mustara Mustara, Hartono Hartono, Eti P Pamungkasari","doi":"10.52225/narra.v5i2.2080","DOIUrl":"10.52225/narra.v5i2.2080","url":null,"abstract":"<p><p>Hypertension is a global health issue with significant effects on morbidity and mortality, and medication adherence is crucial for effective management. Despite its importance, adherence remains low among hypertensive patients. Health education has been shown to improve medication adherence, though its effectiveness varies across studies. The aim of this study was to systematically synthesize evidence on the impact of health education in enhancing medication adherence among hypertensive patients. This study followed preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines and employed the population, intervention, control, and outcome (PICO) approach to develop keywords for a search across five databases: Emerald, ProQuest, PubMed, ScienceDirect, and Scopus. All randomized control trials published between 2019 and 2024 in English, evaluating health education's impact on medication adherence in hypertensive patients aged ≥18 years were included. The protocol was registered on PROSPERO (CRD42024532890), and study quality was assessed using the CEBMa scale. Twelve high-quality articles (CEBMa score of ≥7) involving 1,827 participants were included, identifying four key themes in health education for hypertension: an overview of hypertension and complications, medication and side-effect management, healthy lifestyle modification, and medication adherence strategies. Health education significantly improved medication adherence by 33% (risk ratio (RR): 1.33; 95%CI: 1.08-1.64; <i>p</i>=0.008), with a medium-large effect on improving medication adherence (<i>d</i>=0.70; 95%CI: 0.34-1.05; <i>p</i><0.0001) and a small-medium effect on reducing non-adherence (<i>d</i>=-0.45; 95%CI: -0.66-(-0.24); <i>p</i><0.0001). Health education delivered 1 to 3 months and with individualized approaches showed better adherence outcomes compared to more than three months and group-based methods. Face-to-face education was more effective than the digital method. In conclusion, health education improves medication adherence in hypertensive patients when delivered comprehensively over 1-3 months through individualized face-to-face sessions. These findings support its integration as a key strategy in hypertension management to enhance adherence.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e2080"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mortality and associated factors among community-acquired pneumonia patients: A cross-sectional study in a provincial referral hospital in Indonesia.","authors":"Dani Rosdiana, Fajri M Siregar, Nabila C Ediwi, Rahmi T Putri, Zuyyina Er Nurrahma, Adinda Elisabet, Rosantia Sarassari, Dodi Safari, Cimi Ilmiawati, Aisyah Elliyanti","doi":"10.52225/narra.v5i2.1649","DOIUrl":"10.52225/narra.v5i2.1649","url":null,"abstract":"<p><p>Community-acquired pneumonia (CAP) poses a significant impact on the healthcare system due to rehospitalization and mortality. About one-third of hospitalized CAP patients died within one year. In addition to advanced age, vulnerable groups with comorbidities such as cancer, diabetes, and cerebrovascular disease (CVD) are more likely to suffer from severe CAP. The aim of this study was to investigate the factors linked to mortality in adult hospitalized CAP patients. The study extracted the medical records of patients aged ≥18 years, admitted to a referral hospital in Riau Province, who were diagnosed with CAP between January and December 2023. Multiple logistic regression step-wise analysis was employed to determine the factors associated with mortality in CAP patients. The study involved 334 patients with a median age of 58 years. Based on the confusion, urea, respiratory rate, blood pressure, and age ≥65 years (CURB-65) score, 11.9% of patients had severe CAP (CURB-65 scores 3 and 4). Age was a significant predictor of severe CAP (<i>p≤</i>0.001). The most prevalent comorbidities were malignancy (33.2%), CVD (30.2%), and diabetes (28.4%). Mortality incidence during hospitalization reached 35.9%. Significant factors associated with mortality in hospitalized CAP patients included renal dysfunction/elevated serum urea levels (<i>p</i>=0.031), CURB-65 score (<i>p</i>=0.023), vasopressor use (<i>p≤</i>0.001), mechanical ventilator use (<i>p≤</i>0.01) and steroid use (p=0.029). However, CVD was associated with a decreased risk of mortality (<i>p</i>=0.019). Gram-negative bacteria predominated, accounting for 50.6% of all positive isolates. Several significant factors were associated with mortality in adult patients hospitalized with CAP at referral Hospital in Riau, including renal dysfunction, CURB-65 score, vasopressor use, mechanical ventilator use, and steroid use. This finding underscored the importance of early identification factors in CAP patients.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e1314"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectrum of rare <i>EGFR</i> mutations in Indonesian lung adenocarcinoma: Findings from an 8-year analysis of 4,778 cases highlighting the need for advanced targeted therapies.","authors":"Didik S Heriyanto, Ika Trisnawati, Lisnawati Rachmadi, Jeffry B Tenggara, Vincent Lau, Andrew N Gunawan, Brigitta N Halim, Fara S Yuliani, Vincent Laiman, Soehartati Gondhowiardjo, Hsiao-Chi Chuang","doi":"10.52225/narra.v5i2.1721","DOIUrl":"10.52225/narra.v5i2.1721","url":null,"abstract":"<p><p>Lung cancer patients in Indonesia exhibit a high prevalence of epidermal growth factor receptor (<i>EGFR</i>) mutations, with a substantial proportion attributed to rare or uncommon variants. The clinical significance of rare <i>EGFR</i> mutations lies in their differential sensitivity to tyrosine kinase inhibitors (TKIs). While they are frequently resistant to first- and second-generation TKIs, they often respond to third-generation TKIs, necessitating tailored treatment options. The need for improving access to advanced targeted therapies in Indonesia also highlights the importance of conducting research on rare <i>EGFR</i> mutations. The aim of this study was to identify the spectrum and frequency of <i>EGFR</i> mutations in patients with lung adenocarcinoma in Indonesia. A cross-sectional observational study with total sampling was conducted from January 2016 to April 2024 to investigate <i>EGFR</i> mutation profiles in lung adenocarcinoma patients. Samples were acquired from patients with a confirmed anatomical pathology diagnosis from various healthcare centers across Indonesia. A total of 4,778 samples were analyzed using real-time quantitative polymerase chain reaction (RT-qPCR) on various specimen types to determine <i>EGFR</i> mutation prevalence and patterns. Associations between demographic data and <i>EGFR</i> mutation status were assessed. <i>EGFR</i> mutations were detected in 54.6% of samples, with common mutations (exon 19 deletions/insertions and point mutation L858R) comprising 76.2% of positive cases and rare mutations (exon 20 insertions, point mutation G719X, S768I, T790M, and L861Q) accounted for 20.3%. Significant associations were found between geographic origin, age, and sex with <i>EGFR</i> mutation status. This study confirms substantial genetic variability and geographical differences in <i>EGFR</i> mutations among Indonesian lung adenocarcinoma patients, emphasizing the urgent need for further research to prompt enhanced molecular diagnostics and targeted therapies in the region.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e1721"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative assessment of smartphone-based digital planimetry for wound area measurement.","authors":"Khan Sharun, Shajahan A Banu, Merlin Mamachan, Obli R Vinodhkumar, Kallemuchikal M Manjusha, Rohit Kumar, Abhijit M Pawde, Kuldeep Dhama, Amar Pal","doi":"10.52225/narra.v5i2.1987","DOIUrl":"10.52225/narra.v5i2.1987","url":null,"abstract":"<p><p>Accurate wound area measurement is essential for effective wound care as it helps determine the progression of healing in patients. The aim of this study was to compare two wound area measurement techniques wound tracing (manual planimetry) and imitoMeasure (smartphone-based digital planimetry) with standard ImageJ-based digital image analysis in a rabbit wound healing study. The study involved 291 wounds categorized into small, intermediate, and large wounds. ImageJ was used as the reference method for comparisons. The intraclass correlation coefficient (ICC) was computed to assess the agreement and reliability between different wound measurement techniques. A mountain plot was used to assess the agreement between measurement methods, and a Bland-Altman plot was used to evaluate the agreement and concordance between measurement methods. The time required for analysis (processing time) was also compared. The study revealed that the imitoMeasure consistently demonstrated a greater level of agreement with ImageJ, especially in small and intermediate wounds. The ICC values indicated substantial agreement between ImageJ and imitoMeasure, with an exceptionally high ICC value for small wounds. Mountain plots revealed that the imitoMeasure had better agreement with ImageJ across all wound sizes. Bland-Altman plots further supported these findings, with wound tracing exhibiting wider limits of agreement and greater variability than imitoMeasure. ImitoMeasure consistently proved to be the quickest method across all wound sizes, whereas wound tracing required the longest processing time. These findings indicate that the imitoMeasure is a more reliable and consistent method for measuring the wound area, in particular for small and intermediate wounds.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e1987"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of walking exercise intensity on cartilage IL-1, TNF-α, IL-4, MMP-13 and pain threshold in osteoarthritis rat models.","authors":"Kusworini Handono, Dwi A Prasetyo, Nia Kurnianingsih, Cesarius S Wahono, Thoha M Albaar","doi":"10.52225/narra.v5i2.2109","DOIUrl":"10.52225/narra.v5i2.2109","url":null,"abstract":"<p><p>Pro-inflammatory cytokines produced by chondrocytes play a crucial role in activating matrix metalloproteinase-13 (MMP-13), leading to an imbalance between the synthesis and degradation of the extracellular matrix (ECM) in osteoarthritis (OA). Although regular walking exercise has been shown to reduce inflammatory cytokine levels in OA animal models, the optimal exercise intensity remains underexplored. Therefore, the aim of this study was to investigate the effects of different intensities of regular walking exercise on the levels of pro-inflammatory cytokines (interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-α)), anti-inflammatory cytokine (interleukin-4 (IL-4)), as well as MMP-13 expression in cartilage and pain thresholds in an OA animal model. A total of 30 adult male <i>Rattus norvegicus</i> (6-8 weeks old) were divided into five groups: (1) healthy control; (2) monosodium iodoacetate (MIA)-induce OA model; (3) OA with light-intensity walking (OA1); (4) OA with moderate-intensity walking (OA2); (5) and OA with high- intensity walking (OA3). The exercise intervention began one week after MIA injection and continued for six weeks. Pain threshold, inflammatory cytokine (IL-1, TNF-α, IL-4) levels, and MMP-13 expression were measured using an analgesymeter, enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry (IHC), respectively. The results demonstrated a significant reduction in IL-1 and TNF-α levels, along with decreased MMP-13 expression and increased IL-4 levels, in all exercise groups (OA1, OA2, OA3) compared to the untreated OA group. Additionally, pain thresholds improved following exercise. However, no significant differences were observed among the three exercise intensities in terms of cytokine levels, MMP-13 expression, or pain threshold. This study highlights that the light-intensity regular walking exercise effectively reduces inflammation, MMP-13 expression, and pain in OA. Further research is needed to elucidate the underlying mechanisms of exercise in OA management.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 2","pages":"e2109"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}