Narra J最新文献

{"title":"Low-intensity aerobic cycle ergometer effects on lung function of myasthenia gravis patients: A randomized controlled trial.","authors":"Nabila Amalina, Dewi Poerwandari, Noor I Handajani, Devi A Sudibyo, Soenarnatalina Melaniani","doi":"10.52225/narra.v4i3.844","DOIUrl":"10.52225/narra.v4i3.844","url":null,"abstract":"<p><p>Patients with generalized myasthenia gravis (MG) often show restrictive spirometry results. Although regular exercise and physical fitness are linked to better respiratory function, there is limited research assessing the effects of aerobic exercise on lung function in MG patients. The aim of this study was to analyze the effect of low-intensity aerobic exercise using a cycle ergometer on lung function parameters in MG patients. A randomized controlled trial with pre- and post-test was conducted at the Medical Rehabilitation Outpatient Clinic of Dr. Soetomo General Academic Hospital in Surabaya, Indonesia, in 2023. MG patients classified as I-IIb based on the Myasthenia Gravis Foundation of America (MGFA) classification were recruited and randomly divided into treatment and control groups. The treatment group was given low-intensity aerobic exercise using a cycle ergometer, education on lifestyle changes, and breathing exercises (deep and pursed lip breathing). Lung function parameters, including forced vital capacity (FVC), forced expiratory volume in 1 second (FEV₁), and forced expiratory volume ratio (FEVR), were then measured. Measurements were conducted before and after eight weeks of low-intensity aerobic cycle ergometer exercise and compared with those of a control group. A total of 17 MG patients were included in this study. The results showed a significant increase in FVC in the treatment group (<i>p</i>=0.003), whereas no significant change in the control group (<i>p</i>=0.068). A significant increase in FEV₁ was observed both in the treatment (<i>p</i>=0.029) and the control group (<i>p</i>=0.016). There was no improvement in FEVR in either group. After the intervention, significant differences were observed in FVC (<i>p</i>=0.009) and FEV₁ (<i>p</i>=0.029) between the treatment and control groups. There was no significant difference in FEVR values after the intervention between both groups (<i>p</i>=0.491). In conclusion, eight weeks of low-intensity aerobic cycle ergometer exercise led to significant improvements in FVC and FEV₁ among MG patients.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e844"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>GSTA1</i> gene polymorphisms are associated with cyclophosphamide effectiveness in lupus nephritis patients: A case-control study in Indonesia.","authors":"Yen Ya Indrawijaya, Aluicia A Artarini, Laniyati Hamijoyo, Maria I Iwo","doi":"10.52225/narra.v4i3.1144","DOIUrl":"10.52225/narra.v4i3.1144","url":null,"abstract":"<p><p>Glutathione-S-transferase alpha-1 (<i>GSTA1</i>) is an enzyme with high conjugation activity against aldophosphamide, a metabolite of cyclophosphamide and promoter polymorphisms in <i>GSTA1</i> may influence the cyclophosphamide effectiveness. The aim of this study was to evaluate the effectiveness and side effects of cyclophosphamide in lupus nephritis patients, using <i>GSTA1</i> variants as predictors. A case-control study was conducted at Hasan Sadikin Hospital, Bandung, Indonesia, involving 100 lupus nephritis patients from February 2023 to January 2024. The PCR-Sanger sequencing was used to genotype five selected single nucleotide polymorphisms (SNPs) in the <i>GSTA1</i> promoter: - 52 A > G, -69 T > C, -513 A > G, -567 G > T, and -631 G > T. The endpoint was assessed after six doses of cyclophosphamide by evaluating renal function, disease activity and side effects. Results indicated that six doses of intravenous cyclophosphamide treatment improved renal function and disease activity in the patients, as evidenced by significant changes in serum creatinine (0.79 vs 0.69 mg/dL), dipstick proteinuria (3.00 vs 1.50), creatinine clearance (98.50 vs 109.50 mL/min), and Modified Systemic Lupus Erythematosus Disease Activity Index 2000 (M-SLEDAI-2 K) score (8.61 vs 6.95). The AG genotype at - 513 A > G was associated with reduced cyclophosphamide effectiveness (odds ratio (OR): 0.19; 95%CI: 0.19-0.60; <i>p</i> = 0.019). The GT genotype at -631 G > T independently increased the progression of anemia (OR: 2.41; 95%CI: 0.26-22.12; <i>p</i> = 0.040). This study highlights that the presence of <i>GSTA1</i> variants affected cyclophosphamide effectiveness in lupus nephritis patients, with heterozygous polymorphisms at -513 (AA to AG) and -631 (TT to GT) predicting reduced effectiveness of cyclophosphamide by enhancing <i>GSTA1</i> promoter activity, while anemia further exacerbated lupus nephritis disease severity. <i>GSTA1</i> polymorphism was not associated with the presence of alopecia, amenorrhea, gastrointestinal disorders, and leukopenia during cyclophosphamide therapy.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e1144"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant, anti-collagenase, and antibacterial activities of <i>Fucus vesiculos</i>us silver nanoparticles.","authors":"Evika S Savitri, Annisa E Rahmawaty, Eko B Minarno","doi":"10.52225/narra.v4i3.882","DOIUrl":"10.52225/narra.v4i3.882","url":null,"abstract":"<p><p><i>Fucus vesiculosus</i> is an alga with high fucoxanthin, phlorotannin, fucoidan, sterol, and astaxanthin. The silver nanoparticles of <i>F. vesiculosus</i> (AgNPs-Fv) are expected to have high antioxidant, anti-collagenase, and antibacterial activities. The aim of this study was to characterize the distribution and size of AgNPs-Fv and determine their antioxidant, anti-collagenase, and antibacterial activities. The distribution and size of AgNPs-Fv were measured using particle size analyzer (PSA) analysis. The nanoparticle compound and their functional groups were characterized using a scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR), respectively. The antioxidant activity of AgNPs-Fv was determined using a 1.1-diphenyl-2-picrylhydrazyl (DPPH) assay, while the anti-collagenase activity was examined using the spectrophotometric method. The antibacterial activity was assessed using an inhibition zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). Results indicated that the AgNPs-Fv had a dominant volume of about 86.3% with a diameter of 113.3 nm. SEM analysis revealed the spherical AgNPs with sizes between 27 and 54 nm. The FTIR analysis of the AgNPs-Fv absorption band at 1,046 cm^-1 demonstrated the bond between the Ag metal and the O-H hydroxyl group. The antioxidant activity of AgNPs-Fv was higher than <i>F. vesiculosus</i> extract (24.23±3.55 mg/L vs 47.45±3.16 mg/L). AgNPs-Fv also had a higher anti-collagenase activity compared to <i>F. vesiculosus</i> extract (66.74±6.352 mg/L vs 145.1±6.326 mg/L). The inhibition zone diameter of AgNPs-Fv was greater than <i>F. vesiculosus</i> extract. The MIC and MBC of AgNPs-Fv were 18.75 and 18.75 ppm, while <i>F. vesiculosus</i> extract was 37.5 and 75 ppm, respectively. These results suggested that AgNPs of <i>F. vesiculosus</i> had higher antioxidant, anti-collagenase, and antibacterial activities than <i>F. vesiculosus</i> extract alone.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e882"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors for 30-day mortality in hepatocellular carcinoma patients undergoing liver resection.","authors":"Erik Prabowo, Neni Susilaningsih, Catharina Suharti, Hery D Purnomo, Ignatius Riwanto, Ahmad F Fuadi, Ardiyana Ar, Beatrice LA Bulandari, Kevin C Tjandra, Danendra Rk Respati, Derren Dch Rampengan","doi":"10.52225/narra.v4i3.1001","DOIUrl":"10.52225/narra.v4i3.1001","url":null,"abstract":"<p><p>Hepatocellular carcinoma (HCC) ranks among the most prevalent and fatal liver cancers globally. Liver surgery, particularly resection, offers the potential for cure but poses challenges, especially in Indonesia, where patients often present in advanced stages. This study aimed to determine the intraoperative and perioperative factors associated with 30- day mortality of HCC patients undergoing liver resection at a tertiary referral hospital. The study included HCC patients undergoing liver resection at Karadi General Hospital, Semarang, Indonesia, between January 2018 and September 2023. Demographic data, intraoperative, perioperative, and postoperative factors were collected, with the primary outcome being 30-day mortality. Factors influencing 30-day survival were assessed using a log-rank test and the survival analysis employed Kaplan-Meier curves. Among 58 HCC patients who had liver resection, 62.1% were males, with a mean age was 57.27 ± 9.56 years. Preoperative comorbidities, notably hepatitis B, affected 34.4% of patients. Child-Pugh Score categorized 91.4% as class A. The study found a 30-day mortality rate of 10.3% with no subsequent increase in incidence. The failure-to-rescue rate (FTR) of this study was found 46%. Factors associated with 30-day mortality were Child-Pugh classification (<i>p</i> < 0.001), intraoperative bleeding (<i>p</i> = 0.001), creatinine levels (<i>p</i> = 0.005), Clavien-Dindo classification (<i>p</i> < 0.001), and posthepatectomy liver failure (PHLF) (<i>p</i> < 0.001). This study suggests that preoperative (Child-Pugh classification), intraoperative (blood loss volume), and postoperative factors (Creatinine level, Clavien-Dindo classification, and PHLF) could predict the mortality rate of HCC patients undergoing liver resection.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e1001"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the promising therapeutic benefits of iodine and radioiodine in breast cancer cell lines.","authors":"Aisyah Elliyanti, Nurul Hafizhah, Dhianisa Salsabila, Veronica Y Susilo, Sri Setiyowati, Alimuddin Tofrizal, Yulia Kurniawati, Miftah Irrahmah","doi":"10.52225/narra.v4i3.1078","DOIUrl":"10.52225/narra.v4i3.1078","url":null,"abstract":"<p><p>Iodine has an anti-proliferative effect on cancer cells; however, its effects have not been explored adequately. The aim of this study was to evaluate the therapeutic potential of iodine and radioiodine by assessing their effects on the viability of various breast cancer cell lines: MCF7, SKBR3, and MDA-MB231. The viability of cells was measured in treated cells exposed to six doses of iodine (5, 10, 20, 40, 60, 80 µM) and two doses of radioiodine (3.7 × 10⁴ and 3.7 × 10⁵ Bq). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and modified clonogenic assays were used to assess cell viability. Exposure to 80 µM of iodine significantly reduced the viability of all cell types. The cells were then exposed to a 50% inhibitory concentration (IC₅₀) dose. When the cells were exposed to the IC₅₀ dose of iodine, the MCF7 cell viability was reduced by 42.6 ± 0.14% (IC₅₀ dose 12.88 µM), 40.2 ± 0.08% for SKBR3 (IC₅₀ dose 11.03 µM) and 47.0 ± 0.02% for MDA-MB231 (IC₅₀ dose 14.09 µM). All cells were also exposed to 3.7 × 10⁴ Bq and 3.7 × 10⁵ Bq radioiodine. Both doses significantly reduced the cell viability of MCF7 and SKBR3 cells compared to the unexposed control cells (all had <i>p</i> < 0.05), while MDA-MB231 cell viability only reduced significantly after 3.7 × 10⁵ Bq of radioiodine exposure compared to the unexposed control cells (<i>p</i> < 0.05). This study highlighted that iodine had a toxic effect on breast cancer cells, and radioiodine enhanced the toxicity to breast cancer cells. The types of cancer cells and doses of iodine and radioiodine influenced the effect. These findings suggest that iodine and radioiodine hold promise as therapeutic agents for breast cancer, similar to their established use in thyroid disease treatment. However, further in vivo studies are important to provide more evidence.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e1078"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of early-life malaria exposure on childhood stunting: A case-control study in high endemic malaria area, Papua, Indonesia.","authors":"Muhamad Sahiddin, Hasanuddin Ishak, Andi A Arsin, Mustika Pramestiyani","doi":"10.52225/narra.v4i3.1451","DOIUrl":"10.52225/narra.v4i3.1451","url":null,"abstract":"<p><p>Papua faces public health challenges as a region with high malaria endemicity and a very high prevalence of stunting. Infectious diseases are one of the risk factors for stunting. The aim of this study was to investigate the effect of early-life malaria exposure on stunting among children in Papua. The study was conducted in 14 public health centers (PHCs) in Papua in 2023. Six hundred eighty-one children (227 stunted and 454 non-stunted) were selected using simple random sampling. The study data were gathered from medical records, structured parent interviews, and direct anthropometric measurements of the children. Chi-square tests were performed to determine unadjusted OR, while adjusted OR was calculated using multivariate analysis. The height-for-age z-score was calculated using WHO Anthro version 3.2.2. The results showed that 45.1% of mothers who had malaria during pregnancy had stunted children. The average z-score height-for-age of children from mothers who experienced malaria during pregnancy vs those who did not was -1.69 ± 1.23 vs -1.41 ± 1.55. Among the 84 children who had malaria under one year old, 45.2% experienced stunting. The average z-score height-for-age of children who had malaria under one year old vs those who did not was -1.83 ± 1.24 vs -1.38 ± 1.6. In the unadjusted analysis, malaria during pregnancy (OR 1.74; 95%CI: 1.06-2.87), malaria in children under one year old (OR 1.78; 95%CI: 1.12-2.83), low birth weight status (OR 1.82; 95%CI: 1.08-3.05), family income (OR 1.75; 95%CI: 1.09-2.81), and mother's ethnicity (OR 1.45; 95%CI: 1.05-2.01) were associated with stunting incidence in children. In the multivariate analysis, mother's ethnicity (aOR 1.41; 95%CI: 1.00-1.97) and low birth weight status (aOR 1.72; 95%CI: 1.00-2.94) were the only risk factor for stunting. This study suggests a potential association between early-life malaria exposure and stunting in children. In malaria-endemic areas, health interventions targeting malaria prevention during pregnancy and early childhood are necessary to reduce the risk of stunting.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e1451"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico analysis of <i>Arbacia lixula</i>-derived peptides and plasmid construction for recombinant anti-aging therapies.","authors":"Satya W Yenny, Jamsari Jamsari, Auliya A Hazmi, Kevin N Cuandra, Wafiq Hanifah, Angela S Yahono, Dhyani P Wahyudi, Gherriandi R Buana, Awalil Rk Rahman, Annisa D Maharani, Muhammad F Firjatullah, Rafi Maulana, Norbertus M Prayogi, Christopher D Tristan","doi":"10.52225/narra.v4i3.1283","DOIUrl":"10.52225/narra.v4i3.1283","url":null,"abstract":"<p><p>Skin aging is one of the degenerative processes influenced by tyrosinase, elastase, collagenase, hyaluronidase, and matrix metalloproteinase-9 (MMP9) activity. One promising avenue for discovering antiaging therapeutics is the peptides from the <i>Arbacia lixula</i> spine. The aim of this study was to explore the potential of peptides from <i>A. lixula</i> spine as a multitarget inhibitor for recombinant antiaging therapies through in silico approaches. The crystal structure of peptides previously identified in <i>A. lixula</i> spine was visualized using the UCSF Chimera. The protein data bank (PDB) database was used to obtain the crystal structures of protein targets. The webservers Innovagen, AllerTop, and ToxinPred were utilized to predict the peptide's water solubility, toxicity, and allergenicity. MOE application was used to prepare all ligands and proteins, molecular docking, and visualization. Molecular dynamics simulations were carried out on the protein-ligand complexes on Yasara Dynamics application. The Benchling website was used to perform virtual electrophoresis and reconstruct the recombinant plasmid (Psb1c3). Based on the molecular docking results, peptide REGSPDLLE has the potential as a multitarget inhibitor of tyrosinase (-9.07 kcal/mol), hyaluronidase (-10.57 kcal/mol), elastase (-9.32 kcal/mol), collagenase (-10.57 kcal/mol), and MMP9 (-10.43 kcal/mol). Peptide REGSPDLLE was selected due to its strong binding affinity on the active site of each target protein and exhibits non-toxic, non-allergenic, and good water-soluble as indicated by Support Vector Machine score <0. Molecular dynamics simulations confirmed stable interactions with receptor proteins. Peptide REGSPDLLE was successfully inserted into the recombinant pSB1C3 plasmid, confirmed by virtual electrophoresis with bands at ∼2000bp and ∼150 bp. Further in vitro and in vivo studies are necessary to verify the anti- aging efficacy of peptide REGSPDLLE.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e1283"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long non-coding RNAs as prognostic biomarkers in non-muscle invasive bladder cancer: A systematic review.","authors":"Ahmad Z Hendri, Sri Suryawati, Didik S Heriyanto, Mardiah S Hardianti, Narpati W Pikatan, Sabillal Shaleh, Robert Robert, Toni Febriyanto, Belinda Liliana, Pratama Angga Dm","doi":"10.52225/narra.v4i3.1233","DOIUrl":"10.52225/narra.v4i3.1233","url":null,"abstract":"<p><p>Traditional prognostic tools for non-muscle invasive bladder cancer (NMIBC) often overestimate progression and recurrence risks, underscoring the need for more precise biomarkers. While long non-coding ribonucleic acids (lncRNAs) have been reviewed in bladder cancer, no review has focused on NMIBC. The aim of this study was to address this gap by investigating the role of lncRNAs in predicting NMIBC survival and progression. A systematic review was conducted using PubMed, Scopus, and Cochrane databases as of July 31, 2024. Prognostic studies investigating the association between lncRNA expression and survival outcomes, such as cancer-specific survival, disease-free survival, recurrence-free survival, or overall survival, using Kaplan-Meier curves or hazard ratios, were included. A total of three studies were analyzed, involving 279 NMIBC patients and focusing on three lncRNAs: urothelial cancer associated 1 (UCA1), growth arrest-specific 5 (GAS5), and up-regulated in non-muscle invasive bladder cancer (UNMIBC). Increased UCA1 expression was strongly associated with poor disease-free survival (hazard ratio (HR): 1.974; 95%CI: 1.061-3.673; <i>p</i> = 0.032) and progression-free survival (HR: 3.476; 95%CI: 1.187-10.18; <i>p</i> = 0.023). Reduced GAS5 expression was significantly associated with poor disease-free survival (HR: 2.659; 95%CI: 1.348-5.576; <i>p</i> = 0.005) and progression-free survival (HR: 6.628; 95%CI: 1.494-29.40; <i>p</i> = 0.013). Higher level of UNMIBC was strongly associated with poor recurrence-free survival (HR: 2.362; 95%CI: 1.504-4.837; <i>p</i> = 0.007). In conclusion, lncRNAs have potential as prognostic biomarkers in NMIBC, with UCA1 and UNMIBC overexpression and GAS5 underexpression being significant in predicting disease recurrence and progression, highlighting the clinical relevance of monitoring these lncRNAs to improve prognosis and guide treatment decisions.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e1233"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stem cells for immune modulation in systemic lupus erythematosus: From bench research to clinical applications.","authors":"Andi R Ginting, Delfitri Munir, Mustafa M Amin, Dewi M Darlan, Agung Putra, Muhammad Rusda, Erna Mutiara, Evita Mayasari, Muhammad F Rozi","doi":"10.52225/narra.v4i3.994","DOIUrl":"10.52225/narra.v4i3.994","url":null,"abstract":"<p><p>Systemic lupus erythematosus (SLE) is a prevalent autoimmune disease affecting multiple organ systems. Disease progression is inevitable as part of its natural course, necessitating aggressive therapeutic strategies, particularly with the use of immunosuppressants. Long-term use of steroids and other immunosuppressants is associated with significant adverse effects. Mesenchymal stem cells (MSCs) have been shown to modulate the immune response, leading to immunosuppressive effects against self-antigens. MSCs have demonstrated the ability to modulate several immune cell populations, contributing to favorable outcomes in controlling immune and inflammatory conditions. Recent evidence has shown an increase in Treg and Breg cell subsets following MSC administration, along with modulation of other immune cells, including dendritic cells, B cells, and T cells. However, the balance between MSC pro-inflammatory and anti-inflammatory phenotypic activation remains a critical factor in determining therapeutic outcomes. Various covariates also influence the efficacy of MSC therapy. The aim of this study was to provide a comprehensive overview of the utilization of mesenchymal stem cells (MSCs) in SLE treatment, leveraging their immunomodulatory and immunosuppressive capabilities. Understanding the fundamental preclinical effects of MSCs and recent findings from clinical studies may enhance the potential of MSC therapy in the management of SLE patients.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e994"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity assessment and bioimaging potential of carbon dots synthesized from banana peel in zebrafish model.","authors":"Ni Pad Wijayanti, Fitri A Permatasari, Sophi Damayanti, Kusnandar Anggadiredja, Fery Iskandar, Indra Wibowo, Heni Rachmawati","doi":"10.52225/narra.v4i3.1228","DOIUrl":"10.52225/narra.v4i3.1228","url":null,"abstract":"<p><p>Zebrafish serve as a pivotal model for bioimaging and toxicity assessments; however, the toxicity of banana peel-derived carbon dots in zebrafish has not been previously reported. The aim of this study was to assess the toxicity of carbon dots derived from banana peel in zebrafish, focusing on two types prepared through hydrothermal and pyrolysis methods. Banana peels were synthesized using hydrothermal and pyrolysis techniques and then compared for characteristics, bioimaging ability, and toxicity in zebrafish as an animal model. Pyrolysis-derived banana peel and hydrothermal-derived banana peel showed blue emission under ultraviolet light, indicating excitation-dependent behavior. To test their potential application for bioimaging, a soaking method was used using zebrafish that showed fluorescence intensity in the eyes, abdomen, and tail of zebrafish. Toxicity comparison showed that pyrolysis-derived banana peel had lower toxicity with 50% lethal concentrations (LC₅₀) of 1707.3 ppm than hydrothermal-derived banana peel (LC₅₀ 993 ppm) in zebrafish. Both types of carbon dots showed significant differences (<i>p</i> < 0.05) in hatching rates at 96 and 120 hours of exposure. Of the two methods for carbon dot synthesis from banana peel, the pyrolysis method had a higher toxicity threshold than the hydrothermal method, as indicated by the LC₅₀ value and the number of zebrafish embryos that died, hatched delayed, and experienced malformation during their development.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"4 3","pages":"e1228"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11731817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}