Narra J最新文献

{"title":"Exploring factors contributing to falls in home-dwelling older adults: A cross-sectional study in Northeastern Thailand.","authors":"Laksanee Boonkhao, Nattagorn Choochouy, Pongsak Rattanachaikunsopon, Kitti Laosupap, Thitima Saenrueang, Chiraporn Labcom, Nittaya Chakhamrun, Arun Boonsang, Aree Butsorn","doi":"10.52225/narra.v5i1.1545","DOIUrl":"https://doi.org/10.52225/narra.v5i1.1545","url":null,"abstract":"<p><p>Falls are the most common accidents among older adults in home settings. Older adults experience falls due to several risk factors. In 2005, Thailand became an aging society, with projections indicating that by 2021, older adults would represent the majority of the population, and by 2035, approximately 30 percent of the population would be older adults. Approximately 3 million fall episodes transpire among older adults each year in Thailand, leading to almost 60,000 hospitalizations. The aim of this study was to examine the factors associated with falls among older adults in northeastern Thailand, hypothesizing that characteristics such as cognitive capacity, visual acuity, hearing acuity, balance ability, and mobility are associated with fall risk in this population. A cross-sectional analytical study involved 264 older adults aged 60 years or older using a questionnaire and a battery of tests that assessed the participants' cognitive capacity, eyesight proficiency, hearing ability, balancing ability, and mobility. The variables that exhibited a statistically significant association (<i>p</i> < 0.05) were employed in a binary logistic regression analysis. The results revealed that falls among home-living older adults were significantly associated with sex, family size, congenital issues, and mobility. Older adults who were female and had a large family, congenital disorders, or mobility impairments were at a higher risk of falls, which emphasizes the need for personalized prevention strategies. It is recommended to adopt a proactive healthcare strategy to prevent falls and ensure safe living conditions. Interventions aimed at improving balance, mobility, and mental health, as well as encouraging an active lifestyle, may reduce the risk of falls among older adults living in the community. The findings may aid private and government agencies in developing effective fall prevention programs for older adults living at home.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e1545"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of rotavirus vaccines in Indonesia: A review of genotype distribution and impact.","authors":"Abu T Aman, Afifah Patriani, Yuli Mawarti","doi":"10.52225/narra.v5i1.1681","DOIUrl":"https://doi.org/10.52225/narra.v5i1.1681","url":null,"abstract":"<p><p>Rotavirus remains the leading cause of diarrhea among children under five years of age, with an incidence of 31.1-90.9% in Indonesia. Initially, a rotavirus vaccination program was introduced in several provinces of Indonesia in 2022, which would be conducted nationally. This review provides information on the rotavirus genotype distribution in Indonesia, efficacy and effectiveness data of the rotavirus vaccine, and an update on the status of rotavirus vaccine implementation worldwide. The results show a varied distribution of G and P genotypes from 1978 to 2018, with G1-G3, G9, P[4], P[6], and P[8] as the prevalent genotypes, followed by a small proportion of G4, P[9], P[10], and P[11]. Three rotavirus vaccines, which are prequalified by the World Health Organization (WHO) and available in Indonesia, showed an efficacy of 17.6-76.9% in high-mortality countries. The Indonesian government procured ROTAVAC with a G9P[11] genotype for the national immunization program, which showed 31.3-69.1% protective efficacy against severe gastroenteritis caused by other strains. This review suggested that the decision to choose the rotavirus vaccine for the national program should take into account the country's prevalent circulating genotype and the vaccine's efficacy against severe diarrhea. The use of a pentavalent rotavirus vaccine with high efficacy in high-mortality countries can be regarded as the prime choice for the program. Another alternative is the rotavirus vaccine, which showed efficacy data in multiple high-mortality countries. In addition, regular surveillance of the rotavirus genotypes and the clinical manifestations of diarrhea are necessary to design vaccination strategies in Indonesia.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e1681"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of an <i>E. coli</i>-expressed spike protein-based in-house ELISA system for assessment of antibody responses after COVID-19 infection and vaccination.","authors":"Sitti Nurisyah, Mitsuhiro Iyori, Ammar A Hasyim, Khaeriah Amru, Kei Itani, Kurumi Nakamura, Kartika H Zainal, Handayani Halik, Irawaty Djaharuddin, Agussalim Bukhari, Puji Bs Asih, Din Syafruddin, Shigeto Yoshida, Irfan Idris, Yenni Yusuf","doi":"10.52225/narra.v5i1.1250","DOIUrl":"https://doi.org/10.52225/narra.v5i1.1250","url":null,"abstract":"<p><p>Evaluating long-term immunity after COVID-19 infection and vaccination is critical for managing potential outbreaks. The aim of this study was to develop a cost-effective in-house enzyme-linked immunosorbent assay (ELISA) based on <i>Escherichia coli</i>-expressed SARS-CoV-2 spike protein (E-S1) for antibody detection and to evaluate its performance. The system was validated by comparing the in-house ELISA results with those obtained using a commercial ELISA with HEK293-expressed spike protein (H-S1). Recombinant SARS-CoV-2 spike protein was produced in <i>E. coli</i>, purified, and validated for antigenicity via ELISA. Indirect ELISAs with both E-S1 and H-S1 antigens were performed on 386 serum samples from COVID-19 survivors, vaccinated individuals, and pre-pandemic controls collected at different time points. The E-S1 ELISA showed a statistically significant but weak correlation with H-S1 ELISA across all samples (<i>r</i>=0.205; <i>p</i>=0.0001). Stronger correlations were observed among vaccinated individuals with prior infection on day 90 (<i>r</i>=0.6017; <i>p</i><0.001) and in naïve vaccine recipients on day 30 (<i>r</i>=0.5361; <i>p</i>=0.0003). Pre-pandemic sera from a rural population in Sumba Island exhibited high background reactivity in E-S1 ELISA, likely due to anti-<i>E. coli</i> antibodies, while urban pre-pandemic sera from Jakarta showed a stronger correlation with H-S1 ELISA. This suggests potential regional or immune background differences influencing assay performance. Although E-S1 retained antigenic properties, its diagnostic utility is limited by non-specific reactivity and reduced sensitivity compared to H-S1. In conclusion, <i>E. coli</i> expression systems may not be ideal for producing spike protein-based ELISA antigens specific to SARS-CoV-2. Alternative expression systems, such as human or baculovirus, could enhance diagnostic accuracy and specificity for COVID-19 antibody detection.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e1250"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with mental health disorders in undergraduate students: A meta-analysis.","authors":"Dhian Kartikasari, Sri Andarini, Viera Wardhani, Heni D Windarwati","doi":"10.52225/narra.v5i1.1962","DOIUrl":"10.52225/narra.v5i1.1962","url":null,"abstract":"<p><p>Mental health disorders are a significant issue, yet there is currently no consistent data regarding the factors that may contribute to their occurrence. The aim of this study was to identify the factors that may influence the occurrence of mental health disorders in the undergraduate student population. This meta-analysis, registered in PROSPERO, was conducted as of September 5, 2024. The systematic searches were conducted on three databases (Scopus, Embase, and PubMed). Data on factors related to the occurrence of mental health disorders in undergraduate students were collected to calculate pooled point estimates using the Mantel-Haenszel test. Out of 6,837 articles, 16 articles were included in the analysis. Our findings revealed that female students had a higher chance of experiencing mental health disorders compared to male students (OR: 1.43; 95%CI: 1.14-1.80; <i>p</i>-Egger of 0.1041; <i>p</i>-Heterogeneity of <0.0001; <i>p</i> = 0.0020). The chance of having mental health disorders was also higher among single students compared to those in relationships (OR: 1.20; 95%CI: 1.02-1.43; <i>p</i>-Egger of 0.0535; <i>p</i>-Heterogeneity of 0.1130; <i>p</i> = 0.0180). This study did not find any associations between age group, family income, smoking, living arrangements, years of study, or regularity of physical activity with the occurrence of mental health disorders. These findings could serve as a basis for improving policies aimed at reducing the rates of mental health disorders in undergraduate students.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e1962"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the role of polysaccharides in mitigating organ damage caused by pesticide-induced toxicity: A systematic review and meta-analysis of in vivo studies.","authors":"Elly N Sakinah, Nurud Diniyah, Achmad Subagio, Ancah Cn Marchianti, Jauhar Firdaus, Stela S Fambudi, Stefia A Amini, Dhiani E Putri","doi":"10.52225/narra.v5i1.1553","DOIUrl":"10.52225/narra.v5i1.1553","url":null,"abstract":"<p><p>Although polysaccharides have demonstrated potential in alleviating dysbiosis, the overall impact of polysaccharides on minimizing oxidative stress and organ damage in vivo has not been thoroughly investigated. The aim of this study was to investigate the comprehensive effects of polysaccharides in mitigating pesticide toxicity in animal studies, focusing on biomarkers related to oxidative stress, antioxidant activity, kidney injury, lipid profiles, liver function, and the preservation of liver and kidney weights. A systematic search was conducted across nine indexed databases, including PubMed, Cochrane CENTRAL, Taylor & Francis, Scopus, Sage, EBSCO, ProQuest, ScienceDirect, and Google Scholar. Rayyan.ai was used to screen in vivo studies that met the predefined inclusion and exclusion criteria. The quality of the selected in vivo studies was evaluated using SYRCLE's Risk of Bias tool, specifically designed for animal studies. Thirteen randomized animal studies, comprising 330 mice and rats, were included in the analysis. The findings revealed that polysaccharides significantly increased antioxidant levels, including catalase (CAT) (<i>p</i><0.00001), superoxide dismutase (SOD) (<i>p</i><0.00001), glutathione peroxidase (GPx) (<i>p</i><0.00001), and reduced glutathione (GSH) (<i>p</i><0.00001). Polysaccharides also significantly reduced oxidative stress markers, such as malondialdehyde (MDA) (<i>p</i><0.00001) and nitric oxide (NO) (<i>p</i><0.0001), as well as kidney injury biomarkers, including serum creatinine (<i>p</i><0.00001) and urea (<i>p</i><0.00001). Additionally, improvements in lipid profiles were observed, with significant reductions in triglycerides (TG) (<i>p</i>=0.04) and total cholesterol (TC) (<i>p</i><0.00001). However, there were no significant differences in high-density lipoprotein (HDL) (<i>p</i>=0.28) and low-density lipoprotein (LDL) (<i>p</i>=0.32) levels. Polysaccharides significantly alleviate liver biomarkers, including aspartate transaminase (AST) (<i>p</i><0.0001), alanine transaminase (ALT) (<i>p</i><0.005), and alkaline phosphatase (ALP) (<i>p</i><0.0001). Polysaccharides also contributed to the maintenance of liver weight (<i>p</i>=0.009), although no significant differences were observed in kidney weights (<i>p</i>=0.81). The study highlights that polysaccharides exert significant effects in enhancing antioxidant levels, reducing oxidative stress and organ damage biomarkers, and preserving liver weights.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e1553"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of clove flower extract ( <b><i>Syzygium aromaticum</i></b> ) administration timing on skeletal muscle damage induced by eccentric exercise: An in vivo study.","authors":"Tyas Sr Ningrum, Muchsin Doewes, Dono Indarto, Risya Cilmiaty, Agus Kristiyanto, Vitri Widyaningsih","doi":"10.52225/narra.v5i1.1680","DOIUrl":"https://doi.org/10.52225/narra.v5i1.1680","url":null,"abstract":"<p><p>Eccentric exercise often leads to oxidative stress, inflammation, and muscle damage that impair athletic performance. To counter these adverse effects, clove flower extract (<i>Syzygium aromaticum</i>) offers promising potential as a natural remedy to promote muscle repair with its potent antioxidant and anti-inflammatory properties. The aim of this study was to assess the effects of clove flower extract administration timing on oxidative stress and inflammatory responses in skeletal muscle damage induced by acute eccentric exercise in mice. This study used a post-test-only control group design, involving 35 male mice (<i>Mus musculus</i>, Balb/c) randomly divided into five groups: a healthy control group (HG) with no exercise and no treatment, a negative control group (NG) with exercise but no treatment, T1 group (receiving clove flower extract 24 hours before exercise), T2 group (receiving clove flower extract immediately after exercise), and T3 group (receiving clove flower extract 24 hours after exercise). The treatment groups received a single dose of clove flower extract (500 mg/kg body weight (BW)). The skeletal muscle damage in mice was collected by measuring NADPH oxidase (NOX) and superoxide dismutase (SOD) activities using spectrophotometry, as well as toll-like receptor 4 (TLR4) and interleukin-8 (IL-8) levels using an enzyme-linked immunosorbent assay (ELISA). Moreover, the skeletal muscle damage was analyzed through the histopathological method. Data were analyzed using one-way analysis of variance (ANOVA) followed by Fisher's least significant difference (LSD) tests as a post hoc test. The result showed that clove flower extract significantly reduced NOX (<i>p</i>=0.049) and IL-8 (<i>p</i>=0.032) levels, increased SOD activity (<i>p</i>=0.001), and did not significantly affect TLR4 levels (<i>p</i>=0.532). Moreover, the results showed a significant reduction in muscle damage (<i>p</i>=0.001). The study highlights that the administration of clove flower extract (500 mg/kg BW) 24 hours before exercise, immediately after exercise, or 24 hours after exercise can help prevent muscle damage.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e1680"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144056641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergent roles of circulating miR-133 and miR-155 in modulating angiotensin II levels among hypertensive patients in Melanesian and non-Melanesian populations.","authors":"Shoma A Wijaya, Dwi A Pujianto, Ani R Prijanti, Bambang Widyantoro","doi":"10.52225/narra.v5i1.1759","DOIUrl":"https://doi.org/10.52225/narra.v5i1.1759","url":null,"abstract":"<p><p>The therapeutic approach to hypertension often varies across racial and ethnic groups; however, antihypertensive treatments have not yet been tailored to account for these variations in Indonesia, a country with diverse racial and ethnic groups. In addition, microRNA-133 (miR-133) and microRNA-155 (miR-155) play critical roles in cardiac muscle homeostasis and inflammatory responses, but their specific functions in hypertension remain unclear. The aim of this study was to investigate the correlation between circulating miR-133 and miR-155 levels and angiotensin II (ANG-II) levels in hypertensive patients from Melanesian and non-Melanesian populations in Indonesia. A cross-sectional study was conducted in Jayapura, Indonesia among Melanesian and non- Melanesian hypertensive patients. The levels of ANG-II were quantified using sandwich ELISA, while the relative expression of miR-133 and miR-155 levels were measured by real-time PCR. Differences between the two groups were assessed using the Mann- Whitney test, and correlations between miR and ANG-II levels were determined using the Spearman correlation test. The relative expression levels of miR-133 and miR-155 in the Melanesian group were significantly higher than in the non-Melanesian group; 6.94-fold (3.85 vs 0.55) and 2.1-fold higher (0.19 vs 0.09), respectively. MiR-133 had a moderate negative correlation with ANG-II in both Melanesian (<i>r</i> = -0.538; <i>p</i> < 0.001) and non- Melanesian (<i>r</i> = -0.649; <i>p</i> < 0.001). However, miR-155 had no significant correlation with ANG-II levels in either the Melanesian group (<i>p</i> = 0.551) or non-Melanesian group (<i>p</i> = 0.159). This study highlights that miR-133 levels are significantly correlated with ANG- II concentrations in both Melanesian and non-Melanesian hypertensive patients, suggesting that miR-133 may play a regulatory role in the ANG-II pathway. These findings provide insights into the potential of miR-133 as a biomarker for hypertension management in diverse populations.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e1759"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the power of peptides from <i>Cucumaria frondosa</i> coelomic fluid as multitarget therapy of diabetic kidney disease: An in-silico study.","authors":"Rauza S Rita, Kevin N Cuandra, Syahidatul A Nasri, Mutiara A Carmenita, Nathania A Kristaningtyas, Daffa Z Rasendriya, Rafi Maulana, Muhammad N Hibatullah, Angela S Yahono, Fitrah Afdhal, Filzatuz Z Ibrahim, Balqist K Nayu, Muhammad Teguh","doi":"10.52225/narra.v5i1.1180","DOIUrl":"https://doi.org/10.52225/narra.v5i1.1180","url":null,"abstract":"<p><p>Diabetic kidney disease is a condition characterized by persistent albuminuria, diabetic glomerular lesions, and a reduced glomerular filtration rate in people with diabetes. Peptides in <i>Cucumaria frondosa</i> coelomic fluid have been proven to provide antidiabetic and anti-inflammatory activity that can be used as one of the innovations in developing a multitarget therapy, especially in diabetic kidney disease. Therefore, the aim of this study was to unravel the power of peptide-based metabolites from <i>C. frondosa</i> coelomic fluid as multitarget therapy for diabetic kidney disease using an in-silico study. UCSF Chimera software was utilized to construct the three-dimensional structure of coelomic fluid peptides from <i>C. frondosa.</i> The toxicity and allergenicity of peptides were examined using the ToxinPred and AllerTop websites, respectively. From the PDBJ database, the 3D structures of protein kinase B, alpha isoform (AKT1); vascular endothelial growth factor receptor 2 (VEGFR2); epidermal growth factor receptor (EGFR); α-glucosidase; and glucokinase were obtained. Molecular docking was carried out using MOE Software. In this in-silico study, peptide 9 (-10.32 kcal/mol), peptide 1 (-9.41 kcal/mol), and peptide 3 (-9-55 kcal/mol) were shown to act as specific adenosine triphosphate-competitive inhibitors of EGFR, AKT1, and VEGFR2, respectively. Peptide 8 (-11.06 kcal/mol) can specifically inhibit α-glucosidase by binding to its active site. Peptide 1 (-9.80 kcal/mol) is predicted to specifically inhibit glucokinase activity by blocking its active side. Molecular dynamics simulations confirmed stable interactions with receptor proteins. In conclusion, <i>C. frondosa</i> coelomic fluid peptides have been shown not only to alleviate diabetic kidney disease but also to stabilize blood glucose levels and prevent hyperglycemia based on in-silico analysis.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e1180"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of porang glucomannan combined with a high-protein diet on oxidative stress, inflammation, and aging markers in D-galactose-induced rats.","authors":"Azizah H Safitri, Rahmata A Sayyida, Sigit Setyawan, Nurina Tyagita","doi":"10.52225/narra.v5i1.1995","DOIUrl":"https://doi.org/10.52225/narra.v5i1.1995","url":null,"abstract":"<p><p>Aging is a predominant risk factor for several diseases associated with reduced life expectancy. To address this risk factor, several studies have proposed the combined use of porang glucomannan and a high-protein diet to improve various aging markers. The aim of this study was to determine the effects of porang glucomannan and high-protein combination diet as an anti-aging agent. An experimental study using a post-test-only control group design was conducted using Sprague Dawley white rats. The animals were randomly divided into four groups with different treatments: normal control, D-galactose, high-protein diet, and a combination of porang glucomannan and high-protein combination diet. Blood samples were then collected from the ophthalmic vein on day 58 for biomarker measurement using the enzyme-linked immunosorbent assay (ELISA) method. The parameters measured were superoxide dismutase (SOD), malondialdehyde (MDA), interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), insulin growth factor-1 (IGF-1), NOD-like receptor family pyrin domain-containing protein 3 (NLRP3), growth differentiation factor-11 (GDF11), and α-Klotho levels. The results showed that the combination of porang glucomannan and high-protein diet could improve oxidative stress, inflammation, and aging markers. The analysis of variance (ANOVA) test followed by post-hoc showed significant differences between the combination diet and high protein group (<i>p</i> < 0.001). In addition, the average levels of oxidative stress markers (SOD and MDA) in porang glucomannan and high-protein combination group were improved significantly. Similar results were also obtained for inflammatory markers (IL-6 and TNF-α) and aging markers (NLRP3, IGF-1, GDF-11, and α-Klotho). The mean NRLP-3 levels in glucomannan and high-protein combination group were not significantly different compared to control. The study highlights that the combination of porang glucomannan and a high-protein diet effectively improved various aging markers.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e1995"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual anti-inflammatory and antimicrobial effects of stingless bee propolis on second-degree burns.","authors":"Christian O Manginstar, Trina E Tallei, Christina L Salaki, Nurdjannah J Niode, Hendra K Jaya","doi":"10.52225/narra.v5i1.2359","DOIUrl":"https://doi.org/10.52225/narra.v5i1.2359","url":null,"abstract":"<p><p>Propolis, a natural resinous product from stingless bees, is widely recognized for its anti-inflammatory and antimicrobial properties. However, its combined effects in addressing both inflammation and infection in second-degree burns have remained insufficiently explored. The aim of this study was to investigate the dual role of propolis in modulating inflammation and preventing bacterial infections caused by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and <i>Pseudomonas aeruginosa</i> in a second-degree burn model. Propolis was collected from stingless bees in Gowa, South Sulawesi, Indonesia, and extracted using methanol. Second-degree burns were induced in male <i>Rattus norvegicus,</i> which were then divided into three groups: one treated with propolis, another silver sulfadiazine (positive control), and third with NaCl (negative control). After seven days of treatment, the expression of tumor necrosis factor-alpha (TNF-α) and vascular endothelial growth factor (VEGF) proteins in wound samples was analyzed using immunohistochemistry. The antimicrobial activity of the propolis extract was assessed using the disc diffusion assay, followed by minimum inhibitory concentration (MIC) testing. Network pharmacology analysis was also conducted to assess the anti-inflammatory activity of propolis. Results showed that propolis significantly reduced TNF-α expression and increased VEGF expression, which might enhance VEGF-mediated angiogenesis, leading to improved wound healing compared to controls. The antimicrobial tests demonstrated strong activity against MRSA and <i>P. aeruginosa,</i> with inhibition zones correlating with higher extract concentrations. The MIC value of the propolis extract was 198.66 μg/μL against MRSA and 212.06 μg/μL against <i>P. aeruginosa.</i> Network pharmacology analysis revealed key proteins, including Jun proto-oncogene (JUN), estrogen receptor 1 (ESRi), signal transducer and activator of transcription 3 (STAT3), and proto-oncogene tyrosine-protein kinase Src (SRC), involved in the regulation of TNF-α and VEGF, further supporting the synergistic effects of propolis. This study demonstrates that stingless bee propolis effectively promotes tissue regeneration and prevents infection in second-degree burns, highlighting its potential as an alternative to conventional treatments for wound care.</p>","PeriodicalId":517416,"journal":{"name":"Narra J","volume":"5 1","pages":"e2359"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}