BioMedicine-Taiwan最新文献

{"title":"Imbalances in parathyroid hormones and few electrolytes in patients with renal failure","authors":"Aseel Ibrahim Suhael, Lana Nazar Abdul-Razzaq, Majid M. Mahmood","doi":"10.51248/.v43i3.2820","DOIUrl":"https://doi.org/10.51248/.v43i3.2820","url":null,"abstract":"Introduction and Aim: Chronic kidney disease also referred as chronic renal disease is a condition in which there is a gradual loss of kidney function. End-stage renal failure (ESRD) is the final stage of the disease in which the kidneys cease to function, and the patient continues to depend on long-term dialysis. In this study we aimed to analyze the relationship between parathyroid hormone (PTH), electrolyte disturbances and vitamin D3 in ESRD patients and compare it to healthy individuals.\u0000 \u0000Materials and Methods: 100 outpatients (ages 30-70 years) from Al-Diwaniyah General Hospital and 49 healthy participants (ages 35-65 years) were investigated between July and October 2022.\u0000 \u0000Results: A significant increase in PTH concentrations was observed in the ESRD group 275.16 ± 224.03 pg/ml) when compared to the healthy group (44.42 ± 19.26 pg/ml). Similarly, phosphate concentrations differed significantly between ESRD patients and the healthy group (1.52 ± 0.53 vs. 1.04 ± 0.15 mmol/L, respectively).\u0000A non-significant positive correlation was noticed between PTH and phosphate levels (p value = 0.068; r = 0.253). There was a significant (p-value 0.001) decrease in calcium levels between patients and healthy samples (2.06 ± 0.28 mmol/L vs. 2.36 ± 0.11, respectively), and a negative correlation (r = -0.536) between PTH and calcium levels. The vitamin D3 values showed no significant differences (p > 0.01), between ESRD patients and healthy subjects.\u0000 \u0000Conclusion: A positive correlation existed between serum PTH and phosphate levels in patients with ESRD. Hence these parameters could be monitored on a regular basis to ensure appropriate dietary and medical treatments.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43093244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision medicine advancements in glioblastoma: A systematic review.","authors":"Karan Iyer, Shubham Saini, Suman Bhadra, Sohini Kulavi, Jaya Bandyopadhyay","doi":"10.37796/2211-8039.1403","DOIUrl":"10.37796/2211-8039.1403","url":null,"abstract":"<p><strong>Background: </strong>Glioblastoma multiforme, commonly known as GBM or glioblastoma is a grade IV astrocytoma. Brain tumors are difficult to treat and lead to poor prognosis and survival in patients. Gliomas are categorized into four different grades among which GBM is the worst grade primary brain tumor with a survival of less than a year. The genomic heterogeneity of the brain tumor results in different profiles for patients diagnosed with glioblastoma. Precision medicine focuses on this specific tumor type and suggests specialized treatment for better prognosis and overall survival (OS).</p><p><strong>Purpose: </strong>With the recent advancements in Genome-Wide Studies (GWS) and various characterizations of brain tumors based on genetic, transcriptomic, proteomic, epigenetic, and metabolomics, this review discusses the advancements and opportunities of precision medicine therapeutics, drugs, and diagnosis methods based on the different profiles of glioblastoma.</p><p><strong>Methods: </strong>This review has exhaustively surveyed several pieces of works from various literature databases.</p><p><strong>Conclusion: </strong>It is evident that most primary brain tumors including glioblastoma require specific and precision therapeutics for better prognosis and OS. In present and future, molecular understanding and discovering specific therapies are essential for treatment in the field of neurooncology.</p>","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46886556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of anti-retroviral therapy on baseline serum interleukin-18 levels in HIV-I infected patients relative to viral suppression and CD4+ gain: A prospective pilot study.","authors":"Olayemi Balogun, Bukhari I Shuaib, Abdulrasheed Usman, Aminu A Yusuf, Bolanle O P Musa, Obiako O Reginald, Aliyu A Babadoko","doi":"10.37796/2211-8039.1406","DOIUrl":"10.37796/2211-8039.1406","url":null,"abstract":"<p><strong>Background: </strong>In HIV infection, dysregulation of cytokines, including interleukin 18 (IL-18), has been linked to poor clinical outcomes in studies mainly conducted in resource-rich countries. This phenomenon has not been well-studied in resource-limited settings where outcomes could be confounded by exposure to endemic infections and genetic factors.</p><p><strong>Objectives: </strong>Therefore, the influence of immunological and virological status of HIV-infected, antiretroviral therapy (ART)-naïve patients on serum IL-18 levels at baseline (pretreatment) and 24 weeks following initiation of combination ART (cART24) in a resource-limited setting was investigated.</p><p><strong>Methods: </strong>Using the cross-sectional and longitudinal mixed method design, a total of Forty-four (44) newly diagnosed consenting HIV patients were consecutively recruited during routine clinic visits at the Nasara Treatment & Care Centre of the Ahmadu Bello University Teaching Hospital (ABUTH), Zaria, Nigeria between December 2016 to January 2018, and followed up for 24 weeks on initiation of first-line cART.</p><p><strong>Results: </strong>Serum IL-18 concentrations, CD4+ T-cell counts (CD4+) counts, and HIV1 RNA levels <i>were determined at baseline and cART24. There was little CD4</i>+ <i>count gain in both</i> <200 and ≥ 200 cell/mm³subgroups despite the high proportion of subjects having virological suppression (n = 35, [80%]) at cART24. However, at cART24 there was a more than a threefold decrease in the level of IL-18 concentration compared to baseline in patients with <200 cells/mm³ and a significant decrease in the median plasma IL-18 concentration in patients with HIV1 RNA <1000 cp/mL at cART24. A multivariate logistic regression model shows IL-18 intermediate quartile to be more related to immunological poor gain as compared to the highest quartile.</p><p><strong>Conclusion: </strong>Our study found high baseline and significantly low levels of IL-18 at cART24 in virologically suppressed subjects but not among virological non-suppressed responders despite comparable IL-18 levels by CD4+ T cell count strata at cART24. These findings have implications for risk stratification and treatment outcomes in HIV-positive persons.</p>","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47955662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radial arterial waves for chemotherapy- and radiotherapy-related myocardial damage identification in patients with breast cancer.","authors":"Chia-Ying Lee, Daniela Yunchin Yen, Mark C Hou, Ying-Ling Chen, Rong-Jen Shiau","doi":"10.37796/2211-8039.1390","DOIUrl":"10.37796/2211-8039.1390","url":null,"abstract":"<p><strong>Introduction: </strong>Chemotherapy and radiation therapy for breast cancer cause side effects, such as cardiovascular changes, which can be monitored with echocardiography. However, more convenient methods are always encouraged. Radial arterial waves that are used to detect cardiovascular changes can be used to assist in confirming cardiovascular changes.</p><p><strong>Aim: </strong>This retrospective study aimed to analyze the frequency and time domains of the radial artery pulse wave in patients with breast cancer to understand its effectiveness in identifying cardiovascular changes.</p><p><strong>Methods: </strong>Patients with breast cancer were screened from the pulse examination records in Changhua Christian Hospital and divided into the treatment and remission groups. After unlinking the data, the pulse data were analyzed for the breast cancer treatment and remission group, including the average value of the parameters of four consecutive pulse diagnosis records in four consecutive months to test the difference in pulse waves due to breast cancer treatment between the two groups. Additionally, the pulse wave stability of the two groups was compared using the coefficient of variation.</p><p><strong>Results and conclusion: </strong>The comparison of the pulse wave data between 19 patients in the treatment group and 40 patients in the remission group revealed 45 parameters in time and 50 in frequency domains. D3, ND3, NA1, and NT1 are the four parameters with significant differences (p < 0.05), which are all related to heart function, and mainly related to cardiac output and peripheral resistance, indicating that patients in the treatment period have poor heart function. No difference was found in the degree of data dispersion between the two groups. Cardiovascular side effects caused by breast cancer treatment can mainly be shown in the pulse wave time domain.</p>","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41953973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red cell distribution width, neutrophil lymphocyte ratio and interleukin 10 are good prognostic markers in multiple myeloma.","authors":"Marwa M Seyam, Noha E Esheba, Manal A Eid, Mamdouh A Gabr","doi":"10.37796/2211-8039.1405","DOIUrl":"10.37796/2211-8039.1405","url":null,"abstract":"Background Multiple myeloma (MM) is still an incurable disease so we need to continue developing new diagnostic and prognostic options for its management. There are multiple prognostic factors for MM, but most of them are costly and time consuming. Hence comes the urge to identify bed side and low cost prognostic tools, that is why this study was aiming to identify in Egyptian MM patients. Materials and methods The study was carried on 60 newly diagnosed multiple myeloma patients and 20 age and sex matched healthy individuals as controls. Studied subjects were subdivided into two groups: Group I: 60 multiple myeloma patients which were subdivided into three subgroups: Stage I: 10 patients, Stage II: 17 patients, Stage III: 33 patients, Group II: 20 healthy controls. Results A progressive significant increase in IL-10, RDW, NLR, and beta2 microglobulin (β2M) with disease progression from stage I towards stage III as compared to the control group. However, IL-10, RDW, and NLR have the best prognostic efficiency value regarding to sensitivity, specificity and positive predictive value when compared with β2M. Conclusions IL-10, RDW, and NLR are simple, easy and bedside tests (in the case of RDW, and NLR). They have high sensitivity and specificity when compared to β2M, which is a well-established prognostic factor that highlights the valuable role they play as prognostic markers in MM.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41637742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metformin induces autophagy of cisplatin-resistant human gastric cancer cells in addition to apoptosis.","authors":"Chih-Wun Fang, Jai-Sing Yang, Jo-Hua Chiang, Po-Chuen Shieh, Fuu-Jen Tsai, Chia-Wen Tsai, Wen-Shin Chang","doi":"10.37796/2211-8039.1408","DOIUrl":"10.37796/2211-8039.1408","url":null,"abstract":"<p><p>Metformin has been used to treat cases of type 2 diabetes mellitus, and mounting studies have shown that metformin can act alone or in synergy with other anticancer agents to achieve anti-cancer efficacies on various types of tumors. However, the role of metformin in either inducing autophagy and cisplatin-resistance of human gastric cancer (GC) cells has never been examined. The study has established a cisplatin-resistant GC cell line and investigated the effects of metformin on inducing autophagy on it. The results demonstrated that treatment with metformin can concentration-dependently suppress the cell viability and cell confluence of cisplatin-resistant GC cells, while having no effects on human primary stomach epithelial cells (HPSEC). For the first time, we found that metformin can significantly increase the acidic vesicular organelles (AVO) level and decrease the acridine orange (AO) level spontaneously in the cisplatin-resistant GC cells. Thus, we further checked the other markers, Atg5, Atg12 and LC3-II, which showed that metformin indeed induced autophagy in the cisplatin-resistant GC cells. In addition, treatment of 3-Methyladenine (3-MA) can significantly rescue the metformin-induced autophagy. At the same time, metformin can induce the alterations of apoptosis-associated signal molecules, such as caspase-3 and caspase-7 activities. Overall, the pilot study provided evidence for metformin induced autophagy in addition to apoptosis, making it as an effective anticancer drug for the therapy of cisplatin-resistant GC. Killing the cisplatin-resistant GC cells with non-toxic metformin <i>via</i> both autophagy and apoptosis might extend its usefulness in our fighting with chemo-resistance of gastric cancer cells.</p>","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43148627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The intensity of free radical processes and chaperone activity in the saliva of patients with type 2 diabetes.","authors":"Anna A Cheprasova, Sergey S Popov, Alexander N Pashkov, Aleksei N Verevkin, Valentina O Mittova, Konstantin K Shulgin","doi":"10.37796/2211-8039.1407","DOIUrl":"10.37796/2211-8039.1407","url":null,"abstract":"<p><strong>Introduction: </strong>Saliva is a clinically informative biological fluid that contains many biomarkers, allowing multiple analyses to be performed.</p><p><strong>Aim: </strong>The objectives of this study were the assessment of the serum and saliva levels of biochemical parameters and intensity of free radical processes in T2DM patients and the identification of the correlation between certain criteria.</p><p><strong>Methods: </strong>This case-control study included 40 T2DM patients, which were compared with 40 healthy individuals. The levels of glucose, cholesterol triglycerides, total protein, diene conjugates, and chaperone activity were measured using the spectrophotometric method. The concentration of 8-oxo-2'-deoxyguanosine was assessed by competitive enzyme-linked immunosorbent assay.</p><p><strong>Results: </strong>It was established that the progression of diabetes led to an increase in glucose in saliva. The content of 8-oxo-2'-deoxyguanosine and conjugated dienes increased in serum and this increase was associated with the level of glucose and glycated hemoglobin. The level of protein and chaperone activity increased in the saliva of patients with T2DM compared with the control. The correlation analysis revealed a relationship between total protein concentration and conjugated dienes and between chaperone activity and conjugated dienes in saliva.</p><p><strong>Conclusions: </strong>According to the results of the analysis, the pathological changes in DM affected the salivary glands and their secretions. The obtained results allowed us to recommend using saliva as an alternative to blood for the diagnosis and monitoring of T2DM treatments since it is readily available and quickly responds to changes in metabolism in the body.</p>","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49620203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological evaluation of a polyherbal formulation on testicular function and gonadal histomorphology in exposed Wistar rats.","authors":"Godswill James Udom, Uduak Peter Ise, Omoirri Moses Aziakpono, Ayodeji Aturamu, Mba Ogbonnaya, Israel Kevin Umana, Jude Efiom Okokon","doi":"10.37796/2211-8039.1404","DOIUrl":"10.37796/2211-8039.1404","url":null,"abstract":"<p><strong>Introduction: </strong>Dr Iguedo Goko Cleanser® is a herbal formulation (HF) widely marketed in southern Nigeria and purported to be very efficacious for the management of various diseases including giardiasis, toilet infections, hypertension, diabetes, ulcer, impotence, low libido, low sperm count amongst others. Medicinal plants reportedly produce an array of adverse reactions capable of inducing harmful conditions, including death.</p><p><strong>Aim: </strong>This study evaluated the subchronic toxicity concern of HF on testicular function and gonadal histoarchitecture in Wistar rats.</p><p><strong>Methods: </strong>Thirty Wistar rats of both sexes were randomly divided into six groups (5/group) and were orally administered HF for 60 days. The control groups received 5 mL/kg of distilled water; the treatment groups were administered 476.24 and 158.75 mg/kg body weight of HF each for both male and female rats. Using standard procedures, semen analysis was done for all male rats. Animals were anaesthetised and sacrificed on the 62nd day; the gonads were eviscerated, weighed and fixed in 10% buffered formalin for histopathological examinations.</p><p><strong>Results: </strong>Significant (p < 0.05) increase in sperm count relative to control as well as spermatotoxic effects were observed in male rats. Histologically, the ovary presented some degrees of pathologies: cloggy appearing ovarian cortex with a display of a tumour-like cortical area, scantily displayed primordial follicles, haemorrhagic blood vessels, atretic secondary follicle, and eroding granulosa cells amongst others. Testicular histopathology showed abnormal seminiferous tubules' histoarchitecture, degenerated spermatids, distorted spermatogenic cells' orientation, and displaced spermatids into the luminal space.</p><p><strong>Conclusion: </strong>Herbal drugs are usually regarded to be completely safe due to their natural sources, however, this study discovered exposure-related toxic effects of Dr Iguedo Goko Cleanser® on testicular function and gonadal histomorphology. The findings recommend extreme caution with chronic use and avoidance whenever possible.</p>","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10627203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43784483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory activity of Mentha spicata oils on biofilms of Proteus mirabilis isolated from burns","authors":"Idriss Saleh Jalil, S. Qassim Mohammad, Abdullah Khazal Mohsen, Bahaa Abdullah Laftaah Al-Rubaii","doi":"10.51248/.v43i02.2699","DOIUrl":"https://doi.org/10.51248/.v43i02.2699","url":null,"abstract":"Introduction and Aim: Proteus mirabilis is an opportunistic pathogen, infecting humans, through the release of endotoxins and enzymes such as urease, hemolysin, protease, DNase etc. One of the factors contributing to its virulence is its unusual ability to form crystalline biofilms. This study aimed to investigate the effect of Mentha spicata volatile oil on P. mirabilis biofilm formation.\u0000 \u0000Materials and Methods: P. mirabilis was isolated from infected wound of burns of patients using conventional biochemical tests. Vitek 2-Compact System was used to confirm the diagnosis of bacterial isolates. The isolates were tested for their susceptibility to 11 antibiotics. Aqueous and alcoholic extracts as well as Volatile Oil and natural Menthol extracted from M. spicata were tested for their ability to inhibit biofilm formation by P. mirabilis.\u0000 \u0000Results: In this study 16 out of the 45 burn injury samples were tested positive for P. mirabilis.  Bacterial isolates were found to be resistant to the drugs Levofloxacin and Norfloxacin, with percentages of 10.2% and 11.8%, respectively. Majority of these isolates had the capacity to produce several virulence factors, including biofilm in variable amounts and the enzymes protease, hemolysin, DNase, and gelatinase. The volatile oil and natural component menthol extracted from M. spicata inhibited the formation of biofilm at increasing concentrations.\u0000 \u0000Conclusion: The volatile oil and the natural menthol compound of M. spicata are effective in inhibiting biofilm formation by P. mirabilis.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47790325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification and detection of some virulence and antibiotic genes in Pantoea spp. isolated from clinical samples","authors":"Noor Hameed Shahab, Suha Maher Abed, Maan Hasan Salih","doi":"10.51248/.v43i02.2109","DOIUrl":"https://doi.org/10.51248/.v43i02.2109","url":null,"abstract":"Introduction and Aim: Pantoea species, are Gram negative bacteria that belong to the family Erwiniaceae. These bacteria are opportunistic pathogens known to cause several clinical infections in humans. The purpose of current research was to study the prevalence and identification of Pantoea spp. based on 16S rRNA sequencing, as well as detect the presence of antibiotic resistance and virulence genes in samples isolated from clinical cases.\u0000 \u0000Materials and Methods: For the identification of Pantoea spp., DNA extracted from isolates were subjected to 16s rRNA amplification by PCR. Pantoea isolates were assessed for the presence of virulence (EsaI and ompA), and antibiotic (bacA, oqxB, qnrA, and ermB) genes using specific primers.\u0000 \u0000Results: Three new strains were recorded based on results of 16S rRNA sequencing. The virulence gene Esal was prevalent in all isolates (100%) tested while 15/16 (94%) of isolates had ompA genes. The antibiotic-resistant gene oqxB was found in all isolates (100%), followed by bacA (87.5%), qnrA (75%), and ermB (37%) genes.\u0000 \u0000Conclusion: Pantoea spp isolated from clinical samples were seen to harbor virulence as well as antimicrobial genes indicating the isolates to be pathogenic.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44838479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}