DNA Research最新文献_第5页

Genomic and transcriptomic analyses illuminate the molecular basis of the unique lifestyle of a tubeworm, Lamellibrachia satsuma. 基因组学和转录组学分析阐明了一种管虫独特生活方式的分子基础。

IF 4.1 2区生物学

DNA Research Pub Date : 2023-08-01 DOI: 10.1093/dnares/dsad014

Taiga Uchida, Yuki Yoshioka, Yu Yoshida, Manabu Fujie, Ayuta Yamaki, Akira Sasaki, Koji Inoue, Chuya Shinzato

{"title":"Genomic and transcriptomic analyses illuminate the molecular basis of the unique lifestyle of a tubeworm, Lamellibrachia satsuma.","authors":"Taiga Uchida, Yuki Yoshioka, Yu Yoshida, Manabu Fujie, Ayuta Yamaki, Akira Sasaki, Koji Inoue, Chuya Shinzato","doi":"10.1093/dnares/dsad014","DOIUrl":"https://doi.org/10.1093/dnares/dsad014","url":null,"abstract":"Vestimentiferan tubeworms are representative members of deep-sea chemosynthetic ecosystems. In this study, we developed a draft genome and gene models and performed genomic and transcriptomic analyses of Lamellibrachia satsuma, the only vestimentiferan reported from the euphotic zone. The quality of the genome assembly and gene models is comparable to or higher than those of previously reported vestimentiferan tubeworms. Tissue-specific transcriptome sequencing revealed that Toll-like receptor genes and lineage-specific expanded bacteriolytic enzyme genes are highly expressed in the obturacular and vestimental regions, respectively, suggesting the importance of these tissues in defense against pathogens. On the other hand, globin subunit genes are expressed almost exclusively in the trunk region, supporting the hypothesis that the trophosome is the site of haemoglobin biosynthesis. Vestimentiferan-specific expanded gene families included chitinases, ion channels, and C-type lectins, suggesting the importance of these functions for vestimentiferans. C-type lectins in the trunk region, in particular, may be involved in recognition of pathogens, or in interactions between tubeworms and symbiotic bacteria. Our genomic and transcriptomic analyses enhance understanding of molecular mechanisms underlying the unique lifestyle of vestimentiferan tubeworms, particularly their obligate mutualism with chemosynthetic bacteria.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"30 4","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b0/c3/dsad014.PMC10291997.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10072083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GINGER: an integrated method for high-accuracy prediction of gene structure in higher eukaryotes at the gene and exon level. GINGER：在基因和外显子水平高精度预测高等真核生物基因结构的综合方法。

IF 4.1 2区生物学

DNA Research Pub Date : 2023-08-01 DOI: 10.1093/dnares/dsad017

Takeaki Taniguchi, Miki Okuno, Takahiro Shinoda, Fumiya Kobayashi, Kazuki Takahashi, Hideaki Yuasa, Yuta Nakamura, Hiroyuki Tanaka, Rei Kajitani, Takehiko Itoh

{"title":"GINGER: an integrated method for high-accuracy prediction of gene structure in higher eukaryotes at the gene and exon level.","authors":"Takeaki Taniguchi, Miki Okuno, Takahiro Shinoda, Fumiya Kobayashi, Kazuki Takahashi, Hideaki Yuasa, Yuta Nakamura, Hiroyuki Tanaka, Rei Kajitani, Takehiko Itoh","doi":"10.1093/dnares/dsad017","DOIUrl":"10.1093/dnares/dsad017","url":null,"abstract":"The prediction of gene structure within the genome sequence is the starting point of genome analysis, and its accuracy has a significant impact on the quality of subsequent analyses. Gene structure prediction is roughly divided into RNA-Seq-based methods, ab initio-based methods, homology-based methods, and the integration of individual prediction methods. Integrated methods are mainstream in recent genome projects because they improve prediction accuracy by combining or taking the best individual prediction findings; however, adequate prediction accuracy for eukaryotic species has not yet been achieved. Therefore, we developed an integrated tool, GINGER, that solves various issues related to gene structure prediction in higher eukaryotes. By handling artefacts in alignments of RNA and protein sequences, reconstructing gene structures via dynamic programming with appropriately weighted and scored exon/intron/intergenic regions, and applying different prediction processes and filtering criteria to multi-exon and single-exon genes, we achieved a significant improvement in accuracy compared to the existing integration methods. The feature of GINGER is its high prediction accuracy at the gene and exon levels, which is pronounced for species with more complex gene architectures. GINGER is implemented using Nextflow, which allows for the efficient and effective use of computing resources.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"30 4","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cb/45/dsad017.PMC10439787.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10042560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome-level genome assembly of an endangered plant Prunus mongolica using PacBio and Hi-C technologies. 利用PacBio和Hi-C技术对濒危植物蒙古李染色体水平基因组进行组装。

IF 4.1 2区生物学

DNA Research Pub Date : 2023-08-01 DOI: 10.1093/dnares/dsad012

Qiang Zhu, Yali Wang, Ning Yao, Xilu Ni, Cuiping Wang, Meng Wang, Lei Zhang, Wenyu Liang

{"title":"Chromosome-level genome assembly of an endangered plant Prunus mongolica using PacBio and Hi-C technologies.","authors":"Qiang Zhu, Yali Wang, Ning Yao, Xilu Ni, Cuiping Wang, Meng Wang, Lei Zhang, Wenyu Liang","doi":"10.1093/dnares/dsad012","DOIUrl":"https://doi.org/10.1093/dnares/dsad012","url":null,"abstract":"Prunus mongolica is an ecologically and economically important xerophytic tree native to Northwest China. Here, we report a high-quality, chromosome-level P. mongolica genome assembly integrating PacBio high-fidelity sequencing and Hi-C technology. The assembled genome was 233.17 Mb in size, with 98.89% assigned to eight pseudochromosomes. The genome had contig and scaffold N50s of 24.33 Mb and 26.54 Mb, respectively, a BUSCO completeness score of 98.76%, and CEGMA indicated that 98.47% of the assembled genome was reliably annotated. The genome contained a total of 88.54 Mb (37.97%) of repetitive sequences and 23,798 protein-coding genes. We found that P. mongolica experienced two whole-genome duplications, with the most recent event occurring ~3.57 million years ago. Phylogenetic and chromosome syntenic analyses revealed that P. mongolica was closely related to P. persica and P. dulcis. Furthermore, we identified a number of candidate genes involved in drought tolerance and fatty acid biosynthesis. These candidate genes are likely to prove useful in studies of drought tolerance and fatty acid biosynthesis in P. mongolica, and will provide important genetic resources for molecular breeding and improvement experiments in Prunus species. This high-quality reference genome will also accelerate the study of the adaptation of xerophytic plants to drought.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"30 4","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10254739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9652478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-grafting-induced epigenetic changes leading to drought stress tolerance in tomato plants. 番茄植株自嫁接诱导的抗干旱表观遗传变化。

IF 4.1 2区生物学

DNA Research Pub Date : 2023-08-01 DOI: 10.1093/dnares/dsad016

Maria Isabel Fuentes-Merlos, Masaru Bamba, Shusei Sato, Atsushi Higashitani

{"title":"Self-grafting-induced epigenetic changes leading to drought stress tolerance in tomato plants.","authors":"Maria Isabel Fuentes-Merlos, Masaru Bamba, Shusei Sato, Atsushi Higashitani","doi":"10.1093/dnares/dsad016","DOIUrl":"https://doi.org/10.1093/dnares/dsad016","url":null,"abstract":"Grafting is widely used as a method to increase stress tolerance in good fruiting lines of Solanaceae plants. However, little is known about how grafting, affects epigenetic modifications and leads to stress tolerance, especially within the same line. Here, we studied the effects of self-grafting in tomato plants on histone and DNA modifications and changes in gene expression related to drought stress. We found that at the three-leaf stage, 1 week after self-grafting, histone H3 K4 trimethylation and K27 trimethylation changes were observed in more than 500 genes each, and DNA methylation changes in more than 5,000 gene regions at the shoot apex compared to the non-grafted control. In addition, two weeks after the epigenomic changes, global expression changes continued to be observed at the shoot apex in several genes related to the metabolic process of nitrogen compounds, responses to stimulus, chromosome organization, cell cycle-related genes, and regulation of hormone levels. Finally, these grafted seedlings acquired remarkable drought tolerance, suggesting that epigenomic modifications during the wound-healing process mitigate stress tolerance in tomato plants.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"30 4","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10368339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9877002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of metagenomic workflows using a newly constructed human gut microbiome mock community. 使用新构建的人类肠道微生物组模拟群落评估宏基因组工作流程。

IF 4.1 2区生物学

DNA Research Pub Date : 2023-06-01 DOI: 10.1093/dnares/dsad010

Hiroshi Mori, Tamotsu Kato, Hiroaki Ozawa, Mitsuo Sakamoto, Takumi Murakami, Todd D Taylor, Atsushi Toyoda, Moriya Ohkuma, Ken Kurokawa, Hiroshi Ohno

{"title":"Assessment of metagenomic workflows using a newly constructed human gut microbiome mock community.","authors":"Hiroshi Mori, Tamotsu Kato, Hiroaki Ozawa, Mitsuo Sakamoto, Takumi Murakami, Todd D Taylor, Atsushi Toyoda, Moriya Ohkuma, Ken Kurokawa, Hiroshi Ohno","doi":"10.1093/dnares/dsad010","DOIUrl":"https://doi.org/10.1093/dnares/dsad010","url":null,"abstract":"To quantify the biases introduced during human gut microbiome studies, analyzing an artificial mock community as the reference microbiome is indispensable. However, there are still limited resources for a mock community which well represents the human gut microbiome. Here, we constructed a novel mock community comprising the type strains of 18 major bacterial species in the human gut and assessed the influence of experimental and bioinformatics procedures on the 16S rRNA gene and shotgun metagenomic sequencing. We found that DNA extraction methods greatly affected the DNA yields and taxonomic composition of sequenced reads, and that some of the commonly used primers for 16S rRNA genes were prone to underestimate the abundance of some gut commensal taxa such as Erysipelotrichia, Verrucomicrobiota and Methanobacteriota. Binning of the assembled contigs of shotgun metagenomic sequences by MetaBAT2 produced phylogenetically consistent, less-contaminated bins with varied completeness. The ensemble approach of multiple binning tools by MetaWRAP can improve completeness but sometimes increases the contamination rate. Our benchmark study provides an important foundation for the interpretation of human gut microbiome data by providing means for standardization among gut microbiome data obtained with different methodologies and will facilitate further development of analytical methods.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"30 3","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10229288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9916361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Chromosome-level genome assembly of Lilford's wall lizard, Podarcis lilfordi (Günther, 1874) from the Balearic Islands (Spain). 巴利阿里群岛（西班牙）利尔福德壁蜥 Podarcis lilfordi (Günther, 1874) 染色体级基因组组装。

IF 3.9 2区生物学

DNA Research Pub Date : 2023-06-01 DOI: 10.1093/dnares/dsad008

Jessica Gomez-Garrido, Fernando Cruz, Tyler S Alioto, Nathalie Feiner, Tobias Uller, Marta Gut, Ignacio Sanchez Escudero, Giacomo Tavecchia, Andreu Rotger, Katherin Eliana Otalora Acevedo, Laura Baldo

{"title":"Chromosome-level genome assembly of Lilford's wall lizard, Podarcis lilfordi (Günther, 1874) from the Balearic Islands (Spain).","authors":"Jessica Gomez-Garrido, Fernando Cruz, Tyler S Alioto, Nathalie Feiner, Tobias Uller, Marta Gut, Ignacio Sanchez Escudero, Giacomo Tavecchia, Andreu Rotger, Katherin Eliana Otalora Acevedo, Laura Baldo","doi":"10.1093/dnares/dsad008","DOIUrl":"10.1093/dnares/dsad008","url":null,"abstract":"The Mediterranean lizard Podarcis lilfordi is an emblematic species of the Balearic Islands. The extensive phenotypic diversity among extant isolated populations makes the species a great insular model system for eco-evolutionary studies, as well as a challenging target for conservation management plans. Here we report the first high-quality chromosome-level assembly and annotation of the P. lilfordi genome, along with its mitogenome, based on a mixed sequencing strategy (10X Genomics linked reads, Oxford Nanopore Technologies long reads and Hi-C scaffolding) coupled with extensive transcriptomic data (Illumina and PacBio). The genome assembly (1.5 Gb) is highly contiguous (N50 = 90 Mb) and complete, with 99% of the sequence assigned to candidate chromosomal sequences and >97% gene completeness. We annotated a total of 25,663 protein-coding genes translating into 38,615 proteins. Comparison to the genome of the related species Podarcis muralis revealed substantial similarity in genome size, annotation metrics, repeat content, and a strong collinearity, despite their evolutionary distance (~18-20 MYA). This genome expands the repertoire of available reptilian genomes and will facilitate the exploration of the molecular and evolutionary processes underlying the extraordinary phenotypic diversity of this insular species, while providing a critical resource for conservation genomics.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"30 3","pages":""},"PeriodicalIF":3.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9604292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome sequence and salinity adaptation of the desert shrub Nitraria sibirica (Nitrariaceae, Sapindales). 荒漠灌木西伯利亚白刺(Nitraria sibirica)基因组序列与盐度适应。

IF 4.1 2区生物学

DNA Research Pub Date : 2023-05-11 DOI: 10.1093/dnares/dsad011

Xiaohui Ma, Dafu Ru, Diego F Morales-Briones, Fengyuan Mei, Jingjing Wu, Jianquan Liu, Shengdan Wu

{"title":"Genome sequence and salinity adaptation of the desert shrub Nitraria sibirica (Nitrariaceae, Sapindales).","authors":"Xiaohui Ma, Dafu Ru, Diego F Morales-Briones, Fengyuan Mei, Jingjing Wu, Jianquan Liu, Shengdan Wu","doi":"10.1093/dnares/dsad011","DOIUrl":"https://doi.org/10.1093/dnares/dsad011","url":null,"abstract":"The genetic bases of halophytes for salinity tolerance are crucial for genetically breeding salt-tolerant crops. All natural Nitrariaceae species that exclusively occur in arid environments are highly tolerant to salt stress, but the underlying genomic bases to this adaptation remain unknown. Here we present a high-quality, chromosome-level genome sequence of Nitraria sibirica, with an assembled size of 456.66 Mb and 23,365 annotated genes. Phylogenomic analyses confirmed N. sibirica as the sister to all other sampled representatives from other families in Sapindales, and no lineage-specific whole-genome duplication was found except the gamma triplication event. Still, we found that the genes involved in K + retention, energy supply, and Fe absorption expanded greatly in N. sibirica. Deep transcriptome analyses showed that leaf photosynthesis and cuticular wax formation in roots were enhanced under salt treatments. Furthermore, many transcription factors involved in salt tolerance changed their expressions significantly and displayed tissue- and concentration-dependent signaling in response to salt stress. Additionally, we found vacuolar Na + compartmentalization is an ongoing process under salt treatment, while Na + exclusion tends to function at high salt concentrations. These genomic and transcriptomic changes conferred salt tolerance in N. sibirica and pave the way for future breeding of salt-tolerant crops.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2b/78/dsad011.PMC10211498.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9894892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of tandem repeats in the Capsicum annuum genome. 辣椒基因组串联重复序列的检测。

IF 4.1 2区生物学

DNA Research Pub Date : 2023-04-25 DOI: 10.1093/dnares/dsad007

Valentina Rudenko, Eugene Korotkov

{"title":"Detection of tandem repeats in the Capsicum annuum genome.","authors":"Valentina Rudenko, Eugene Korotkov","doi":"10.1093/dnares/dsad007","DOIUrl":"https://doi.org/10.1093/dnares/dsad007","url":null,"abstract":"In this study, we modified the multiple alignment method based on the generation of random position weight matrices (RPWM) and used it to search for tandem repeats (TRs) in the Capsicum annuum genome. The application of the modified (m)RPWM method, which considers the correlation of adjusting nucleotides, resulted in the identification of 908,072 TR regions with repeat lengths from 2 to 200 bp in the C. annuum genome, where they occupied ~29%. The most common TRs were 2 and 3 bp long followed by those of 21, 4, and 15 bp. We performed clustering analysis of TRs with repeat lengths of 2 and 21 bp and created position-weight matrices (PWMs) for each group; these templates could be used to search for TRs of a given length in any nucleotide sequence. All detected TRs can be accessed through publicly available database (http : //victoria.biengi.ac.ru/capsicum_tr/). Comparison of mRPWM with other TR search methods such as Tandem Repeat Finder, T-REKS, and XSTREAM indicated that mRPWM could detect significantly more TRs at similar false discovery rates, indicating its superior performance. The developed mRPWM method can be successfully applied to the identification of highly divergent TRs, which is important for functional analysis of genomes and evolutionary studies.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10211496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9528783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Telomere-to-telomere genome assembly of matsutake (Tricholoma matsutake). 松茸(Tricholoma matsutake)端粒-端粒基因组组装。

IF 4.1 2区生物学

DNA Research Pub Date : 2023-04-25 DOI: 10.1093/dnares/dsad006

Hiroyuki Kurokochi, Naoyuki Tajima, Mitsuhiko P Sato, Kazutoshi Yoshitake, Shuichi Asakawa, Sachiko Isobe, Kenta Shirasawa

引用次数: 0

Chromosome-level genome assembly of Phrynocephalus forsythii using third-generation DNA sequencing and Hi-C analysis. 利用第三代DNA测序和Hi-C分析的连翘蕨染色体水平基因组组装。

IF 4.1 2区生物学

DNA Research Pub Date : 2023-04-01 DOI: 10.1093/dnares/dsad003

Yue Qi, Wei Zhao, Yangyang Zhao, Chenkai Niu, Shuhui Cao, Yingmei Zhang

{"title":"Chromosome-level genome assembly of Phrynocephalus forsythii using third-generation DNA sequencing and Hi-C analysis.","authors":"Yue Qi, Wei Zhao, Yangyang Zhao, Chenkai Niu, Shuhui Cao, Yingmei Zhang","doi":"10.1093/dnares/dsad003","DOIUrl":"https://doi.org/10.1093/dnares/dsad003","url":null,"abstract":"Phrynocephalus forsythii is a viviparous sand lizard that is endemic to the Tarim Basin with a broad altitudinal range of 872-3,100 m. Such variation in altitude and ecological variables can offer an opportunity to uncover genetic mechanisms of ectothermic adaptation to extreme environments at high- and low-altitude. Furthermore, the evolutionary relationship of karyotype with two different chromosome numbers (2n = 46 or 2n = 48) in the Chinese Phrynocephalus is unclear. In this study, a chromosome-level reference genome of P. forsythii was assembled. The genome assembly size was 1.82 Gb with a contig N50 length of 46.22 Mb, 20,194 protein-coding genes were predicted and 95.50% of these genes were annotated in functional public databases. After cluster contigs into chromosome level using Hi-C paired-end reads, we found that two chromosomes of P. forsythii were originated from one ancestral chromosome of species with 46 chromosomes. Comparative genomic analysis revealed that numerous characteristics associated with high- or low-altitude adaptation, including energy metabolism pathways, hypoxic adaptation, and immune, exhibit rapid changes or show signals of positive selection in the P. forsythii genome. This genome provides an excellent genome resource for the study of the karyotype evolution and ecological genomics of Phrynocephalus.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":"30 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10113879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9330727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0