Comparative Immunology Microbiology and Infectious Diseases最新文献

{"title":"First report on the seroprevalence of Egg Drop Syndrome Virus-76 in laying hen flocks in Algeria","authors":"Omar Salhi ,&nbsp;Samia Ameziane ,&nbsp;Mohamed Rahal ,&nbsp;Mustapha Nabi ,&nbsp;Meriem Tahraoui ,&nbsp;Chafik Redha Messaï ,&nbsp;Aziz Lounas ,&nbsp;Nassim Ouchene ,&nbsp;Nadjet Amina Khelifi Touhami","doi":"10.1016/j.cimid.2025.102317","DOIUrl":"10.1016/j.cimid.2025.102317","url":null,"abstract":"<div><div>Egg Drop Syndrome (EDS-76), caused by an avian hemagglutinating adenovirus, results in economic losses by reducing egg production and eggshell quality. This study investigated EDS-76 occurrence in 35 Algerian commercial laying hen flocks (10,000–150,000 birds, aged 24–62 weeks), identified risk factors, and assessed diagnostic methods. A total of 1400 birds were sampled, and their serum was analyzed using indirect ELISA to evaluate seroprevalence and contributing factors. Out of 35 tested flocks, 19 (54.28 %) were seropositive. Highest prevalence (48.57 %, p &lt; 0.01) was observed in summer. Significant strain differences were observed (p &lt; 0.01), with ISA Brown showing the highest prevalence (42.85 %) and Hy-line the lowest (14.28 %). Higher bird density per cage (&gt;5 birds) was associated with increased prevalence (85.71 %, p &lt; 0.0001), as was inadequate hygiene (65.71 %, p &lt; 0.001). Vaccination had a protective effect, reducing prevalence (40 % vs. 73.33 %, p &lt; 0.0001). High mortality rates (&gt;5 %) and severe (&gt;40 %) or prolonged (&gt;3 weeks) egg-laying drops significantly increased risk (p &lt; 0.03). Abnormal eggshell quality was strongly linked to high prevalence (80 %, p &lt; 0.0001). Timing of egg-laying drops also mattered, with the peak laying period showing the highest prevalence (57.14 %, p &lt; 0.04). To prevent and manage EDS-76, it is crucial to enhance biosecurity and hygiene, reduce bird density, implement tailored vaccination programs, and conduct regular serological surveillance. Optimized management during summer, farmer awareness, and targeted national policies will help minimize economic losses and improve productivity in commercial laying hen flocks.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"118 ","pages":"Article 102317"},"PeriodicalIF":2.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143336175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Listeria monocytogenes from different sources: The serotyping, genotyping, virulotyping, and antibiotic susceptibilities of the recovered isolates","authors":"Emre Karakaya ,&nbsp;Fuat Aydin ,&nbsp;Kadir Semih Gümüşsoy ,&nbsp;Tuba Kayman ,&nbsp;Özgür Güran ,&nbsp;Cansu Güran ,&nbsp;Doğancan Yarim ,&nbsp;Enes Said Gündüz ,&nbsp;Seçil Abay","doi":"10.1016/j.cimid.2025.102314","DOIUrl":"10.1016/j.cimid.2025.102314","url":null,"abstract":"<div><div>It was aimed at serotyping, genotyping, determining various virulence genes, and investigating antibiotic susceptibilities of <em>Listeria monocytogenes</em> isolates recovered from different sources in the current study. For this purpose, a total of 70 <em>L. monocytogenes</em> isolates including 22 chicken, 20 fish, 18 sheep, and 10 cattle origin were used. Polymerase Chain Reaction (PCR) was performed for serotyping and analysis of virulence genes of the isolates, and also Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was performed for the genotyping. In addition, it was determined the susceptibilities of the isolates against nine different antibiotics via the disk diffusion method. As a result of serotyping, the most detected serogroup in analyzed <em>L. monocytogenes</em> isolates was 1/2a-3a (44.3 %), and but the least detected serogroup 1/2b-3b-7 (11.4 %). ERIC-PCR results revealed a total of 18 different patterns. All isolates were positive for the presence of <em>inl</em>A, <em>inl</em>B, <em>inl</em>C, <em>iap</em>, <em>prf</em>A, <em>act</em>A, <em>hly</em>, <em>plc</em>A, <em>plc</em>B and <em>mpl</em> virulence genes tested. The prevalence of the <em>act</em>A gene in isolates was determined as 70 %. Antibiotic resistance was detected against six antibiotics, and high resistance to oxacillin (80 %) and ciprofloxacin (65.7 %) in the isolates. Furthermore, the rate of multi-drug resistance in <em>L. monocytogenes</em> isolates was 28.5 % (20/70). In conclusion, the present study showed that the sources may pose a potential health risk, according to obtained data on the virulence gene prevalence, serogroup distribution, high genetic heterogeneity, and antibiotic resistance profiles of <em>L. monocytogenes</em> isolates from different sources.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"118 ","pages":"Article 102314"},"PeriodicalIF":2.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143336174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First identification of Brucella melitensis and Rickettsia monacensis in a red fox, Vulpes vulpes","authors":"Rachid Selmi ,&nbsp;Hanène Belkahia ,&nbsp;Mariem Ben Abdallah ,&nbsp;Aymen Mamlouk ,&nbsp;Monia Daaloul-Jedidi ,&nbsp;Mourad Ben Said ,&nbsp;Lilia Messadi","doi":"10.1016/j.cimid.2025.102315","DOIUrl":"10.1016/j.cimid.2025.102315","url":null,"abstract":"<div><div>Wild canids, in particular red foxes, serve as potential carriers of zoonotic pathogens. This study aimed to investigate the presence of somepathogenic bacteria relevant to veterinary public health concern. On November 15, 2023, we collected spleen and blood samples from a corpse of a red fox found randomly during a field trip in the El-Jouza district, Beja governorate, northern Tunisia. PCR amplification assays were conducted using specific primers to detect <em>Brucella</em> spp. and major vector-borne bacteria, including <em>Rickettsia</em>, <em>Anaplasma</em>, <em>Borrelia</em>, <em>Bartonella</em>, <em>Coxiella</em>, and hemotropic <em>Mycoplasma</em>. Species identification and genetic characterization were performed through BLAST analysis and phylogenetic studies following the sequencing of the obtained PCR products. DNA of both <em>Brucella melitensis</em> and <em>Rickettsia monacensis</em> was detected in the blood sample. Phylogenetic analysis revealed that the identified <em>B. melitensis</em> isolate was clustered with two strains isolated from humans in Mediterranean countries. The <em>R. monacensis</em> isolate was found to be genetically close to a human strain from a South Korea and several isolates infecting<em>Ixodes</em> ticks primarily in Europe. This study revealed for the first time the potential infection of red foxes by <em>B. melitensis</em> and <em>R. monacensis</em>. Further research with larger sample sizes is needed to comprehensively understand the range of zoonotic bacteria in wildlife and to develop effective monitoring and control strategies.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"117 ","pages":"Article 102315"},"PeriodicalIF":2.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey on the prevalence of trypanosomosis in small ruminants and on tsetse distribution in Dedesa and Bedele districts, Buno Bedele Zone, Ethiopia","authors":"Tsegaye Wolde Oche ,&nbsp;Yigremachew Kasahun","doi":"10.1016/j.cimid.2025.102304","DOIUrl":"10.1016/j.cimid.2025.102304","url":null,"abstract":"<div><h3>Background</h3><div>African animal trypanosomosis (AAT) is one of the most serious diseases with ongoing detrimental effects on animal health and food production.</div></div><div><h3>Methods</h3><div>A cross-sectional study was conducted in the Bedele and Dedesa districts of Buno Bedele Zone, Southwest Ethiopia, to determine the prevalence of trypanosomosis and its vector distributions in small ruminants. Blood samples collected from a total of 384 small ruminants were examined for trypanosomosis via hematological analysis. For the tsetse distribution survey, 72 traps were deployed in the designated districts.</div></div><div><h3>Results</h3><div>Among the small ruminants examined with hematological techniques (n = 384), 16 (8 sheep and 8 goats) were infected with trypanosomes. The overall prevalence of trypanosomosis was 4.17 %. Only two species of trypanosomes, namely, <em>T. vivax</em> and <em>T. congolense,</em> were observed in this study, with equal prevalence rates (2.08 %). The mean PCV ± SD of the parasitemic small ruminants (24.37 ± 5.66) was significantly lower than that of the aparasitemic (27.48 ± 4.38) small ruminants. A total of 369 flies were caught (<em>Glossina tachinoides</em> (74.5 %) and <em>G. morsitans</em> (25.5 %)), with an overall mean apparent density of 2.56 flies per trap per day.</div></div><div><h3>Conclusions</h3><div>Overall, these results indicate that this disease is prevalent in these animals, and maintaining their health may play a significant role in defending the disease's spread. Greater emphasis needs to be given to controlling this disease in these ruminants, and the role of these animals as carriers of the disease needs to be considered in future planning and strategies of African animal trypanosomosis control activities.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"117 ","pages":"Article 102304"},"PeriodicalIF":2.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of a high dose of isometamidium chloride treatment in single and mixed experimental infections with T. congolense and T. brucei brucei in dogs","authors":"Romanus C. Ezeokonkwo ,&nbsp;Chukwunonso F. Obi ,&nbsp;Michael I. Okpala ,&nbsp;Chijioke N. Iheagwam ,&nbsp;Ikenna O. Ezeh","doi":"10.1016/j.cimid.2025.102303","DOIUrl":"10.1016/j.cimid.2025.102303","url":null,"abstract":"<div><div>Canine African trypanosomosis is endemic in sub-Saharan Africa. Chemotherapy remains the commonly employed approach to trypanosomosis control. However, it is beleaguered by the absence of new drugs, treatment failures, relapse infection and resistance. The efficacy of a high dose of isometamidium chloride (ISM) in single and mixed infections of <em>T. congolense</em> and <em>T. brucei brucei</em> therapy was assessed in dogs. Fifteen dogs employed in this study were allocated into four groups at random, each with four dogs except group I which had three dogs. Group I dogs were not infected while groups II and III dogs received 10⁶ <em>T. congolense</em> and <em>T. brucei brucei</em> respectively. Group IV dogs received both (5 × 10⁵) <em>T. congolense</em> and <em>T. brucei brucei</em>. Groups II-IV dogs were dosed with 1 mg/kg ISM (Trypamidium-Samorin®) intraperitoneally on day 14 post-infection (PI). Parasitaemia levels, live body weight changes (LBWC), clinical signs, rectal temperature (RT), some haematological and serum biochemical parameters were used to evaluate the efficacy of high dose of ISM. Following infection, all the infected dogs became parasitaemic by the 14th day PI, with obvious clinical signs. Treatment with ISM cleared parasitaemia within 72 hours post-treatment, caused the reversal of the clinical signs, and enhanced the RT, LBWC, haematological and serum biochemical parameters of the dogs. Relapse infection was not recorded throughout the study duration (84 days post-infection). In conclusion, 1 mg/kg of ISM is effective in treating African trypanosomosis in dogs and should be adopted as a first-line treatment for the disease.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"117 ","pages":"Article 102303"},"PeriodicalIF":2.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reptile trade and chelonians-associated Salmonellosis in humans: A public health concern","authors":"Randa Benabdallah ,&nbsp;Hani Saoudi ,&nbsp;Faouzi Matallah ,&nbsp;Valentina Virginia Ebani ,&nbsp;Ammar Ayachi ,&nbsp;Leila Aoun","doi":"10.1016/j.cimid.2025.102305","DOIUrl":"10.1016/j.cimid.2025.102305","url":null,"abstract":"<div><div>Animal trade has become a serious criminal practice in the world. Every day thousands of exotic wild animals, including reptiles, are farmed and sold worldwide. The illegal collection of turtles and tortoises remains completely unsupervised and represents a big challenge for responsible authorities<strong>.</strong> This has consequences for animals and public health because it can contribute to the spread of multiple zoonotic pathogens by introducing exotic species into new geographical areas like wet markets, pet shops, and houses. Non-typhoidal <em>Salmonella</em> are bacteria that can commonly cause self-limiting gastroenteritis but can also cause more serious symptoms, especially in more vulnerable people with impaired immune systems. There is growing evidence linking <em>Salmonella</em> to reptiles, especially chelonians, and its prevalence has increased recently as turtles and tortoises have become popular as new pets. These animals can potentially transmit antimicrobial resistant strains of <em>Salmonella</em> to humans, which puts people in danger, especially young children. Pet owners should be aware and take all the control measures to prevent the infection. This updated review investigates the reptile trade situations worldwide, including chelonians, explaining their relationship with the spread of <em>Salmonella</em> and the risk of infections for humans.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"117 ","pages":"Article 102305"},"PeriodicalIF":2.0,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic and molecular analysis of hemagglutinin gene and Fsp-coding region of canine distemper virus: Insight into novel vaccine development","authors":"Mojtaba Khosravi ,&nbsp;Fatemeh Zahra Gharib ,&nbsp;Alireza bakhshi","doi":"10.1016/j.cimid.2024.102292","DOIUrl":"10.1016/j.cimid.2024.102292","url":null,"abstract":"<div><div>Canine distemper virus (CDV) causes a highly contagious and lethal disease in a vast range of carnivorous and non-carnivorous species. The study aimed to genetically investigate the hemagglutinin (H) gene and Fsp-coding region of CDV isolates from vaccinated dogs. Phylogenetic analysis of the H gene and Fsp-coding region showed that our viruses belonged to the Arctic-like lineage which was distinct from two commonly used vaccine strains (America-1 lineage strains) in Iran. Our data presented a high similarity between the H gene sequences of studied viruses. The multiple sequence alignment of the H gene of our viruses against vaccine strains revealed 91.3–95.6 % and 89.9–94.4 % in the level of nucleotide and amino acid identity, respectively. Our finding identified a potential recombination breakpoint occurring between codons 520–607, along with three positive selection sites including residues 415,547, and 549 among the H gene using the Data Monkey platform. A significant variation of B cell epitopes was found in Hemagglutinating and noose epitope (HNE), with respect to America-1 vaccine strains. Moreover, the H genes of studied viruses had 8 N-glycosylation sites, which is more than the America-1 vaccine strains. Our results confirmed that the circulation of Arctic-like lineage may be a prevalent lineage. Despite widespread vaccination, it does not provide full protection against CDV infection. Due to antigenic differences between our viruses and commonly used vaccine strains, it seems a novel vaccine strain is needed to prevent and prepare full protection against Arctic-like CDV infection.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"117 ","pages":"Article 102292"},"PeriodicalIF":2.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First record of the presence of antibodies against Leptospira in Lagostomus maximus, and Leptospira weillii serogroup Celledoni in Chaetophractus villosus, Argentina","authors":"Marta Susana Kin ,&nbsp;María C. Sanchez ,&nbsp;Micaela Esteban ,&nbsp;Mara L. Martinez ,&nbsp;Bibiana F. Brihuega ,&nbsp;Micaela Hamer ,&nbsp;Vanina Saraullo ,&nbsp;Eduardo D. Cayron ,&nbsp;Gabriel P. Cayron ,&nbsp;Hugo Giménez ,&nbsp;Carlos R. Cayron ,&nbsp;Marcelo Fort","doi":"10.1016/j.cimid.2025.102302","DOIUrl":"10.1016/j.cimid.2025.102302","url":null,"abstract":"<div><div>Leptospirosis is a zoonosis with a worldwide distribution, which is transmitted through direct contact with infected animals or by contact with contaminated water or soil. Objectives was to examine the presence of antibodies against 23 serogroups of <em>Leptospira</em> in <em>Lagostomus maximus</em> and <em>Chaetopractus villosus</em> in La Pampa province, Argentina. Ninety-four serum samples from <em>L. maximus</em> (80) and <em>C. villosus</em> (14) were taken. The samples were analysed by microscopic agglutination test (MAT). Only 5/94 (5.3 %) serum samples presented antibodies against <em>Leptospira</em> sp. Three (21.4 %) serum samples from <em>C. villosus</em>, were reactive to the serogroups, Ballum, Grippotyphosa, Canicola and Celledoni, and two samples (2.5 %) of <em>L. maximus</em> to Pomona, Canicola and Ballum. This constitutes the first record of the presence of antibodies serogroup Celledoni in <em>C. villosus</em> and serogroups Pomona, Canicola and Ballum in <em>L. maximus</em> in Argentina.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"117 ","pages":"Article 102302"},"PeriodicalIF":2.0,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feline immunodeficiency virus (FIV): Prevalence, risk factors, and clinical findings in domestic cats (Felis catus) from southern Brazil","authors":"Lauren Santos de Mello ,&nbsp;Laura Pancich Diesel ,&nbsp;Weslei de Oliveira Santana ,&nbsp;Nilo Ikuta ,&nbsp;André Salvador Kazantzi Fonseca ,&nbsp;Diéssy Kipper ,&nbsp;Raquel Redaelli ,&nbsp;Vagner Reinaldo Zingali Bueno Pereira ,&nbsp;André Felipe Streck ,&nbsp;Vagner Ricardo Lunge","doi":"10.1016/j.cimid.2024.102285","DOIUrl":"10.1016/j.cimid.2024.102285","url":null,"abstract":"<div><div>Feline immunodeficiency virus (FIV) is a retrovirus affecting domestic cats worldwide and causing immunosuppression and reduced quality of life. The prevalence of FIV infection varies according to geographic regions / countries and it is associated with domestic cat health managements (vaccination, neutering, basic health care, etc.). This study aimed to evaluate prevalence, risk factors and some clinical information of FIV infection in a domestic cat population in southern Brazil. A total of 366 cats from ten different veterinary facilities and 43 neighborhoods located in the city of Caxias do Sul were sampled for this study. Each animal was examined by a clinical veterinarian and blood was collected for laboratory analysis. Blood samples were tested with a point-of-care (POC) assay to detect FIV antibodies and a real-time polymerase chain reaction (qPCR) to detect and quantify FIV proviral DNA. The results demonstrated an overall prevalence of 7.1 % (n = 26; 95 % CI = 4.9 % – 10.2 %) of FIV infection. FIV-positive cats demonstrated undetectable (n = 6; 23.1 %) and detectable (n = 20; 76.9 %) proviral DNA, with loads varying from less than 10 (10¹) to 4460,000 (10^6.6) with a mean of 480,000 (10^5.7) copies/mL. FIV-positive cats were significantly older (median age of 7 years), also presenting outdoor access (OR: 5.0, <em>p</em> = 0.046), feline leukemia virus (FeLV) coinfection (OR: 7.1, <em>p</em> &lt; 0.001) and other chronic diseases (OR: 4.1, <em>p</em> = 0.34) as main risk factors. FIV-positive animals presented a 9.9- and 7.6-times higher risk for developing lymphoma and anemia in the multivariate analysis. This study contributes to assess some important epidemiological aspects of FIV infection in cats in southern Brazil, highlighting the strong association of FIV and FeLV coinfection.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"116 ","pages":"Article 102285"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling of antimicrobial resistance genes from Staphylococcus pseudintermedius isolated from dogs with pyoderma using whole genome sequencing","authors":"D.K. Parasana ,&nbsp;I.H. Kalyani ,&nbsp;A.V. Kachchhi ,&nbsp;P.G. Koringa ,&nbsp;P.M. Makwana ,&nbsp;D.R. Patel ,&nbsp;U.V. Ramani ,&nbsp;B.B. Javia ,&nbsp;S.N. Ghodasara","doi":"10.1016/j.cimid.2024.102288","DOIUrl":"10.1016/j.cimid.2024.102288","url":null,"abstract":"<div><div><em>Staphylococcus pseudintermedius</em> is considered as a most common cause of canine pyoderma. Antimicrobial resistance is considered as a global public health concern. The main aim of the study was to evaluate the distribution of Antimicrobial resistance genes in <em>S. pseudintermedius</em> isolates using whole genome sequencing. In the current work, Antimicrobial susceptibility testing and Whole genome sequencing was performed on Five <em>S. pseudintermedius</em> isolates recovered from canine pyoderma cases presented at Veterinary Clinical Complex, Navsari. Bioinformatics analysis of sequencing data was performed to detect Antimicrobial resistance genes, plasmids and mobile genetic elements in <em>S. pseudintermedius</em> isolates. All <em>S. pseudintermedius</em> isolates shows resistance towards amikacin, ampicillin, cefpodoxime, cephalexin, erythromycin and trimethoprim. Higher sensitivity was observed against clindamycin (4/5, 80 %). A total 15 AMR genes, one plasmid (repUS43), two insertion sequences (ISLgar5 and IS256) and one Integrative Conjugative Element (Tn6009) were detected using whole genome sequencing analysis. This study aids us to know about relationship between antimicrobial resistance and presence of AMR genes, plasmids and mobile genetic elements.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"116 ","pages":"Article 102288"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}