Comparative Immunology Microbiology and Infectious Diseases最新文献

{"title":"Dynamics of high pathogenicity avian influenza virus infection with multiple introductions in a crow flock in an urban park in Hokkaido, Japan","authors":"Norikazu Isoda ,&nbsp;Takahiro Hiono ,&nbsp;Yik Lim Hew ,&nbsp;Fumihito Takaya ,&nbsp;Bao Linh Nguyen ,&nbsp;Daiki Kobayashi ,&nbsp;Kaien Fujino ,&nbsp;Yoshihiro Sakoda","doi":"10.1016/j.cimid.2025.102367","DOIUrl":"10.1016/j.cimid.2025.102367","url":null,"abstract":"<div><div>Since 2021, high pathogenicity avian influenza viruses (HPAIVs) of the H5N1 clade 2.3.4.4b has been circulating globally, not only in domestic poultry but also in wild birds, both migratory and resident species. In March to May 2022, March to April 2023, and January to April 2024, crow die-offs were reported in an urban garden in Hokkaido, Japan, raising suspicions of HPAIV infection. Since August 2022, all dead carcasses were investigated for HPAIV detection and isolation. Phylogenetic analysis of the H5 hemagglutinin gene revealed that all detected HPAIVs belonged to clade 2.3.4.4b, a dominant lineage in Hokkaido since early 2022. Two distinct subgroups were identified: G2d (in 2022–2024) and G2a (in 2024). A maximum clade credibility tree, based on concatenated nucleotide sequences of the isolates, suggested that multiple distinct types of HPAIVs were introduced into the garden in rotation during the winters of 2022–2023 and 2023–2024. Infectious HPAIVs were isolated not only from the lungs and brains but also from the rectal contents of the dead crows, with no apparent difference in viral titers between the two subgroups. The case reproduction numbers of HPAIV infection in the crow flock ranged from 0.52 and 1.57 in the spring of 2022 and from 0.55 to 1.78 in the spring of 2023, suggesting that the contiguous HPAIV infections in the crows were due to multiple introductions into the flock. Crow can play a key role of potential spread to other animals, poultry and wildlife in urban areas or humans in rural areas.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"121 ","pages":"Article 102367"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Chlamydia psittaci in pet parrots sold in Xining, China","authors":"Zeqi Liang ,&nbsp;Te Ba ,&nbsp;Lin Liang","doi":"10.1016/j.cimid.2025.102368","DOIUrl":"10.1016/j.cimid.2025.102368","url":null,"abstract":"<div><div><em>Chlamydia psittaci</em> (<em>C. psittaci</em>) is a zoonotic pathogen, causing a systemic wasting disease in avian as well as lung infections in humans. To evaluate the risks of psittacosis posed through pet birds, 182 fecal samples were collected from commercially popular parrot species, including budgerigars (n = 87), cockatiels (n = 28), and lovebirds (n = 67), in Xining City, northwestern China. Through molecular methods, results revealed that an overall <em>C. psittaci</em> prevalence of 14.3 % (26/182), while species-specific infection rates of 21.8 % (19/87) in budgerigars, 10.7 % (3/28) in cockatiels, and 6.0 % (4/67) in lovebirds. Phylogenetic analysis <em>ompA</em>, 16S rRNA, and 16–23S rRNA intergenic spacer (IGS) gene sequence obtained in this study confirmed that all sequences were classified to the <em>Chlamydiaceae</em> family and showed a high similarity to <em>C. psittaci</em> strains 6BC, that was previously identified in avian. This is the first documented report of <em>C. psittaci</em> prevalence in the pet bird markets on the Qinghai-Tibet Plateau. These findings provided an essential baseline data for understanding the epidemiology of <em>C. psittaci</em> in northwestern China and highlighted the need for surveillance to mitigate potential zoonotic risks.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"121 ","pages":"Article 102368"},"PeriodicalIF":2.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144178592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of Leishmania spp. in pigs farms (Sus scrofa domesticus): Epidemiological insights and zoonotic implications","authors":"Orla Ballesteros-García ,&nbsp;Lola Martínez-Sáez ,&nbsp;Ana Lesta ,&nbsp;Pablo Jesús Marín-García ,&nbsp;Ana Martínez-Guillen ,&nbsp;Lola Llobat","doi":"10.1016/j.cimid.2025.102365","DOIUrl":"10.1016/j.cimid.2025.102365","url":null,"abstract":"<div><div>Leishmaniasis is a zoonotic disease caused by the <em>Leishmania</em> spp. an intracellular parasite which are transmitted by phlebotomine sand flies. Dogs have traditionally been considered the primary reservoir for <em>Leishmania infantum</em>, predominant agent in the Mediterrenean region. However, this parasite has also been identified in different host species, including wild animals and livestock. The role of farm pigs in the epidemiology of leishmaniasis remains unclear. The objective of this study has been to assess the seroprevalence of <em>Leishmania</em> spp. in farm pigs (<em>Sus scrofa domesticus</em>) from intensive farms in the Eastern Spain, a Mediterranean region. To carry out this study, a total of 94 pigs were tested for <em>Leishmania</em> spp. antibodies by serological test, and epidemiological data were collected regarding sex, age, reproductive status, farm type, and location. The overall seroprevalence of <em>Leishmania</em> spp. was 4.3 %, with a higher prevalence in weaned piglets (2.1 %) compared to new-born piglets and adults (1.1 %). A higher of seroprevalence was found in farrow-to-wean farms than in finishing farms. For the first time, <em>Leishmania</em> spp. antibodies have been detected in farm pigs of Europe, suggesting that these animals could play a role in the maintenance and transmission of this parasite in endemic areas. The results highlight the importance of further studies related to the epidemiology of leishmaniasis including in livestock, and the potential implementation of vector control strategies in farms to reduce transmission risk.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"121 ","pages":"Article 102365"},"PeriodicalIF":2.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report on the molecular characterization and phylogenetic analysis of meq oncogene from virulent serotype-1 Marek's disease virus in laying hen flocks and broiler breeders in Algeria","authors":"Samia Ameziane ,&nbsp;Omar Salhi ,&nbsp;Nabila Hammami ,&nbsp;Salim Zaidi ,&nbsp;Amina Amraoui ,&nbsp;Aya Bensalem ,&nbsp;Meriem Tahraoui ,&nbsp;Nassim Ouchene ,&nbsp;Nadjet Amina Khelifi Touhami ,&nbsp;Sana Hireche ,&nbsp;Amir Agabou","doi":"10.1016/j.cimid.2025.102358","DOIUrl":"10.1016/j.cimid.2025.102358","url":null,"abstract":"<div><div>Marek's disease (MD) is a highly contagious viral infection in poultry, caused by Gallid alphaherpesvirus 2 (GaHV-2), better known as serotype 1 of Marek’s disease virus (MDV-1). It is one of the most concerning diseases in the poultry industry due to its significant economic impact. The disease mainly affects chickens, although other avian species can also be affected. The objective of this study was to examine the occurrence of Marek's disease virus (MDV) in fourteen poultry farms in Algeria through autopsy, histopathological analysis and molecular characterization of Marek's disease virus. This study represents one of the first investigations combining macroscopic, histopathological, and molecular analyses to assess the prevalence and genetic diversity of Marek's disease virus in Algerian poultry farms. A survey to assess the current occurrence of Marek's disease in poultry farms in Algeria was conducted from June to December 2024. Tissue samples were collected from 70 chickens, including 35 from broiler breeder farms and 35 from layer farms. Macroscopic and histopathological examinations were performed. Macroscopic pathological alterations, such as hepatomegaly, splenomegaly, renal hypertrophy, and sciatic nerve enlargement with follicular atresia and lymphomatous infiltration, were observed. Histopathological analyses revealed diffuse and multifocal whitish infiltration of the spleen, neoplastic infiltration of the liver, intrafollicular lymphoid infiltration in the bursa of Fabricius, and infiltration in the sciatic nerves. The molecular characterization of MDV through PCR amplification and sequencing of the <em>meq</em> gene provides novel insights into the circulating strains in Algeria. The samples were screened by PCR amplification of the <em>meq</em> gene. Additionally, we sequenced the <em>meq</em> gene from five samples per flock, and a phylogenetic tree was constructed using the deduced amino acid sequences of the <em>meq</em> gene. This study is the first to identify and classify MDV strains in Algerian broiler breeder and laying hen farms, revealing the presence of very virulent (vv) MDV pathotypes. Marek's disease remains a major veterinary and economic challenge in the poultry industry, requiring ongoing research to better understand its mechanisms, genetic variations, and control methods.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"120 ","pages":"Article 102358"},"PeriodicalIF":2.0,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunization of BALB/c mice with detoxified lipopolysaccharide and hydrolytic O-polysaccharide from Brucella melitensis either in combination with or conjugated to tetanus toxoid, enhances protective immune responses against the pathogen","authors":"Nima Khoramabadi ,&nbsp;Reza Hosseini Doust ,&nbsp;Ashraf Mohabati Mobarez ,&nbsp;Reza Shapouri","doi":"10.1016/j.cimid.2025.102357","DOIUrl":"10.1016/j.cimid.2025.102357","url":null,"abstract":"<div><div>Lipopolysaccharide (LPS) is the major surface antigen of <em>Brucella</em>, an intracellular pathogen that causes brucellosis in both animals and humans. A deeper understanding of the immune responses elicited by this key antigen may offer valuable insights for the development of effective vaccines for use in both humans and animals. In this study, detoxified LPS (d-LPS) and hydrolytic O-polysaccharide (OPS) from <em>B. melitensis</em> were prepared and separately conjugated to tetanus toxoid (TT) as a carrier protein. The resulting conjugates, d-LPS-TT and OPS-TT, as well as mixture of d-LPS+TT and OPS+TT, were used to immunize separate groups of BALB/c mice. The conjugated antigens induced significant IgG2a-specific serum responses targeting the polysaccharide components. Furthermore, mice immunized with d-LPS-TT and OPS-TT demonstrated elevated levels of IL-12 and IFN-γ following intraperitoneal challenge with <em>B. melitensis</em> 16 M. Notably, the strongest protective immune responses were observed in mice receiving the d-LPS-TT. Most previous studies have attributed protective responses primarily to specific serum antibodies. Although antibodies against Brucella polysaccharides typically associated with T-helper 2 (Th2) type responses, develop during infection, they are insufficient to eliminate the intracellular pathogen from the host. While the precise mechanism remain to be fully elucidated, our findings suggest that immunization with covalently conjugated polysaccharide antigens may promote T-helper 1(Th1) type cellular immunity, which appear to play a more pivotal role in protection against <em>B. melitensis</em>.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"121 ","pages":"Article 102357"},"PeriodicalIF":2.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and characterization of Leptospira species in bats and other small wild mammals from Villeta municipality, Colombia","authors":"Carlos Ramiro Silva-Ramos ,&nbsp;J. Manuel Matiz-González ,&nbsp;C. Alexander Barrero-Rubiano ,&nbsp;Juan Diego Villar ,&nbsp;Jerson Andrés Cuéllar-Sáenz ,&nbsp;Camila López-Rivera ,&nbsp;Laura Natalia Robayo-Sánchez ,&nbsp;José J. Henao-Osorio ,&nbsp;Alexandra Cardona-Giraldo ,&nbsp;Julián A. Mejorano-Fonseca ,&nbsp;Piedad Agudelo-Flórez ,&nbsp;Jesús Alfredo Cortés-Vecino ,&nbsp;Álvaro A. Faccini-Martínez ,&nbsp;Claudia Cuervo ,&nbsp;Héctor E. Ramírez-Chaves ,&nbsp;Marylin Hidalgo ,&nbsp;Alejandro Ramírez-Hernández","doi":"10.1016/j.cimid.2025.102355","DOIUrl":"10.1016/j.cimid.2025.102355","url":null,"abstract":"<div><div><em>Leptospira</em> is a bacterial genus which includes several pathogenic species. Wild mammals can act as reservoir hosts, shedding bacteria in their urine. Leptospirosis is an important health problem in Villeta, but data regarding potential reservoirs hosts and natural sources of infection are still scarce. We aimed to detect and characterize the presence of <em>Leptospira</em> among small wild mammals from Villeta municipality, Colombia. Small wild mammals from three orders: Didelphimorphia, Chiroptera and Rodentia, were sampled in the region. DNA was extracted from kidney samples and screened for <em>Leptospira</em> through real-time PCR targeting a the 16 s rRNA gene. Positive samples were screened through conventional PCR using five complementary genes: <em>adk</em>, <em>icdA</em>, <em>lipL32</em>, <em>lipL41</em> and <em>secY</em>. Amplicons were sequenced and used for phylogenetic analysis. A total of 75 kidney samples were collected from three small wild mammal orders: 62 bats, 5 opossums, and 7 rodents. <em>Leptospira</em> spp. was detected in 38.7 % of the samples, with bats presenting the most frequent infection rate (43.5 %). The infection rate varied by sampling site, with the highest frequency observed in Mave village (57.1 %). Concatenated phylogenetic analysis revealed that all sequences clustered within the P1/Pathogenic <em>Leptospira</em> major clade, forming three subclades: two bat-related <em>Leptospira</em> subclades, and one subclade including <em>Leptospira interrogans</em>, <em>Leptospira kirschneri</em>, and <em>Leptospira noguchii</em> species. This study describes the presence of <em>Leptospira</em> among bats and opossums from Villeta, Colombia. It also identifies the circulation of several P1/pathogenic <em>Leptospira</em> species among bats forming three clusters, two of them composed exclusively of bat-related leptospires, and one together with recognized pathogenic species.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"120 ","pages":"Article 102355"},"PeriodicalIF":2.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of enteric viruses in symptomatic and asymptomatic dogs in Gujarat, India","authors":"Sejal P. Antiya ,&nbsp;Arun C. Patel ,&nbsp;Kishan Kumar Sharma ,&nbsp;H.C. Chauhan ,&nbsp;Sandip S. Patel ,&nbsp;Sushil Kumar Mohapatra ,&nbsp;Mehul D. Shrimali ,&nbsp;Harshkumar A. Patel","doi":"10.1016/j.cimid.2025.102356","DOIUrl":"10.1016/j.cimid.2025.102356","url":null,"abstract":"<div><div>In the recent past, canine astrovirus (CaAstV) and SARS-CoV-2 have emerged as newly identified agents contributing to canine viral gastroenteritis alongside established pathogens <em>viz</em>. canine coronavirus (CCoV), canine parvovirus (CPV), and canine distemper virus (CDV). To study the molecular epidemiology of these viruses in India, faecal samples (n = 168) were collected from diarrhoeic and healthy dogs and history of animals was recorded. Viruses were identified with real-time PCR using virus-specific primers. Sequencing of CaAstV and CPV positive amplicons was done for confirmation of diagnosis and to know the prevalent strains/variants of viruses in the study area, respectively. 75.60 % (127/168) of the samples were positive for intended viruses, and CPV-2 (73.80 %) was found as the most prevalent virus, followed by CDV (11.90 %), CaAstV (5.95 %), SARS-CoV-2(3.57 %) and CCoV (1.19 %). In clinically healthy dogs, CPV-2, CDV, CaAstV, SARS-CoV2 and CCoV were recorded in 57.73 %, 7.21 %, 3.09 %, 1.03 % and 0 % of samples, respectively. In diarrhoeic dogs, the prevalence of CPV-2, CDV, CaAstV, SARS-CoV2, and CCoV was found to be 95.77 %, 18.30 %, 9.86 %, 7.04 % and 2.81 %, respectively. Co-infections were present in 27.56 % (35/127) of the positive samples, CPV-2 and CDV formed the most prevalent combination. CDV and CPV infection was also seen in incompletely or fully vaccinated dogs.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"121 ","pages":"Article 102356"},"PeriodicalIF":2.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schistosoma japonicum cystatin attenuated CLP-induced sepsis in mice though inducing tolerogenic dendritic cells and regulatory T cells","authors":"Yi-Xiang Wang ,&nbsp;Yi-Dan Ma ,&nbsp;Hui-Hui Li ,&nbsp;Wen-Juan Duo ,&nbsp;Qi-Wang Jin ,&nbsp;Kai-Jun Zhou ,&nbsp;Yan-Ran Gao ,&nbsp;Jun-Nan He ,&nbsp;Yu-Jie Xie ,&nbsp;Liang Chu ,&nbsp;Xiao-Di Yang","doi":"10.1016/j.cimid.2025.102345","DOIUrl":"10.1016/j.cimid.2025.102345","url":null,"abstract":"<div><div>Sepsis is a life-threatening complication caused by the overwhelming immune response to bacterial infection leading to the fatal organ damage and even death. Helminth infections modulate host’s immune system through secreting functional proteins to reduce host immune attack as a survival strategy, therefore have been used for the therapy of some inflammatory or autoimmune diseases. <em>Sj</em>-Cys is a cysteine protease inhibitor secreted by <em>Schistosoma japonicum</em> exerting strong immunomodulatory function which has been used to treat sepsis, however, the mechanism underlying the therapeutic efficacy has not been fully elucidated. In this study, we expressed <em>Sj</em>-Cys as recombinant protein (r<em>Sj</em>-Cys) in prokaryotic system and r<em>Sj</em>-Cys was used to incubate with mouse bone marrow derived dendritic cells (BMDCs) <em>in vitro</em>. Our study revealed that r<em>Sj</em>-Cys was able to induce differentiation of BMDCs to tolerant property (TolDCs). Adoptive transfer of r<em>Sj</em>-Cys induced-TolDCs into mice with cecal ligation and puncture (CLP)-induced sepsis conferred a significant therapeutic effect on CLP-induced sepsis in mice with reduced mortality and vital organ damage. The therapeutic effect of <em>Sj</em>-Cys-induced TolDCs was associated with upregulation of CD3⁺CD4⁺CD25⁺Foxp3⁺ regulatory T cells (Tregs) and reduced inflammatory cytokines IL-6 and TNF-α and boosted level of regulatory cytokines IL-10 and TGF-β. The results identified in this study further suggest r<em>Sj</em>-Cys has the potential to be developed into a drug substance for the treatment of inflammatory or autoimmune diseases due to its immunomodulatory effect on tolerant dendritic cells and regulatory T cells.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"120 ","pages":"Article 102345"},"PeriodicalIF":2.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and risk factor modeling of marek’s disease virus in indigenous tilili breed chickens of the Amhara Region, Ethiopia","authors":"Kassahun Berrie ,&nbsp;Wudu Temesgen ,&nbsp;Animaw Shite ,&nbsp;Bemrew Admassu ,&nbsp;Achenef Melaku ,&nbsp;Mastewal Birhan ,&nbsp;Kalkidan Getenet ,&nbsp;Melkie Dagnaw Fenta ,&nbsp;Mebrie Zemene Kinde ,&nbsp;Abebe Belete Bitew ,&nbsp;Asnakew Mulaw ,&nbsp;Yesuneh Tefera Mekasha","doi":"10.1016/j.cimid.2025.102341","DOIUrl":"10.1016/j.cimid.2025.102341","url":null,"abstract":"<div><div>This study aims to assess the seroprevalence and potential risk factors associated with Mareke’s disease occurrence in indigenous Tilili breed chickens and its crosses. A cross-sectional serological study and questionnaire survey were conducted in seven districts of the Amhara region, Ethiopia, from November 2023 to June 2024. Using multi-stage sampling, 17 Kebeles (peasant associations) were selected from the seven districts. A total of 768 chicken sera were sampled and tested for presence of Mareke’s disease virus antibodies using an indirect enzyme-linked immunosorbant assay test. According to the study, the overall apparent seroprevalence of MDV was 74.6 %, with Ankesha district having the lowest prevalence (57.5 %) and Banja district having the highest (93.8 %).The prevalence varied significantly across the studied districts, and there was a statistically significant variation in disease distribution among the peasant associations. Key risk factors associated with Marek’s disease virus seropositivity in Tilili breeds included contact with other birds in the vicinity, sex, age group, and sanitation of the housing. However, factors such as breed, rearing method, and farming type were not significantly associated with Marek’s disease sero-status. This high seroprevalence of the disease in this chicken ecotype indicates active circulation of the virus in the area, underscoring the need for stringent control measures. Further molecular studies and identification of MDV DNA in active outbreaks is critical to characterize the circulating virus and assess the economic impact of the disease on these valuable indigenous chickens of Ethiopia.</div></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"120 ","pages":"Article 102341"},"PeriodicalIF":2.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Comparative efficacy of recombinant VP6 protein based in-house Latex Agglutination test with other diagnostic assays for detection of Rotavirus A from calves, piglets and children” [Comp. Immunol. Microbiol. Infect. Dis. 119 (2025) 102336]","authors":"Bilal Ahmad Malla ,&nbsp;Zunjar Baburao Dubal ,&nbsp;Ajay Kumar ,&nbsp;Obli Rajendran VinodhKumar ,&nbsp;Aquil Mohmad ,&nbsp;Pashupathi Mani ,&nbsp;Kaushal Kishor Rajak ,&nbsp;Kiran Narayan Bhilegaonkar","doi":"10.1016/j.cimid.2025.102339","DOIUrl":"10.1016/j.cimid.2025.102339","url":null,"abstract":"","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"120 ","pages":"Article 102339"},"PeriodicalIF":2.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}