Stresses最新文献_第3页

Regulation, Biosynthesis, and Extraction of Bacillus-Derived Lipopeptides and Its Implications in Biological Control of Phytopathogens 芽孢杆菌衍生脂肽的调控、生物合成和提取及其在植物病原体生物防治中的意义

Stresses Pub Date : 2024-01-16 DOI: 10.3390/stresses4010007

Valeria Valenzuela Ruiz, Azucena Gándara-Ledezma, María Fernanda Villarreal-Delgado, Eber Villa-Rodríguez, F. I. Parra-Cota, Gustavo Santoyo, L. J. Gómez-Godínez, Luis A. Cira Chávez, S. de los Santos-Villalobos

{"title":"Regulation, Biosynthesis, and Extraction of Bacillus-Derived Lipopeptides and Its Implications in Biological Control of Phytopathogens","authors":"Valeria Valenzuela Ruiz, Azucena Gándara-Ledezma, María Fernanda Villarreal-Delgado, Eber Villa-Rodríguez, F. I. Parra-Cota, Gustavo Santoyo, L. J. Gómez-Godínez, Luis A. Cira Chávez, S. de los Santos-Villalobos","doi":"10.3390/stresses4010007","DOIUrl":"https://doi.org/10.3390/stresses4010007","url":null,"abstract":"In recent years, the adoption of sustainable pest management strategies has increased interest in the utilization of biopesticides, with a focus on harnessing beneficial microorganisms. Among these, lipopeptides, such as surfactins, iturins, and fengycins produced by the genus Bacillus, have gained significant attention due to their multifaceted biocontrol mechanisms and wide-ranging inhibitory effects. This review aims to address the regulation, biosynthesis, and production of three main lipopeptide families secreted by the genus Bacillus, as well as the identification and quantification analysis used to date, through the omic tools approach. The three families have been identified as key contributors to the biocontrol abilities of these bacteria, with their broad-spectrum activity making them valuable tools in integrated pest management approaches that aim to reduce reliance on chemical pesticides use while maintaining crop health and productivity.","PeriodicalId":508968,"journal":{"name":"Stresses","volume":" 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139620112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplementary Light on the Development of Lettuce and Cauliflower Seedlings 补充光照对莴苣和菜花幼苗生长的影响

Stresses Pub Date : 2024-01-10 DOI: 10.3390/stresses4010006

Adilson Antonio Rizzon, W. Silvestre, C. B. Vicenço, L. Rota, G. Pauletti

{"title":"Supplementary Light on the Development of Lettuce and Cauliflower Seedlings","authors":"Adilson Antonio Rizzon, W. Silvestre, C. B. Vicenço, L. Rota, G. Pauletti","doi":"10.3390/stresses4010006","DOIUrl":"https://doi.org/10.3390/stresses4010006","url":null,"abstract":"The production of seedlings is one of the main activities for implementing agricultural crops. Many factors are involved in producing quality seedlings, including nutrition, health, genetics, and climatic factors such as temperature, humidity, and light. To evaluate the effect of light supplementation, a study was conducted using supplementary artificial light to produce lettuce and cauliflower seedlings. Sowing was carried out in styrofoam trays under a floating irrigation system. Part of the experiment containing the two species, received treatment with LED light for an additional 4 h per day, in addition to solar radiation (10 h∙day−1). The remaining seedlings received only solar radiation (without supplementation). After 37 days, the seedlings’ biometric (leaf area, root length, aerial dry mass, and root dry mass) and biochemical parameters (phenolic compounds, flavonoids, chlorophyll a/b, and total chlorophyll) were analyzed. The data showed that the complementary light enhanced the performance in all the biometric parameters evaluated in the experiment for lettuce and cauliflower. The biochemical parameters in lettuce were also higher in seedlings with light supplementation. For cauliflower, supplementary light did not differ from the natural photoperiod for biochemical parameters except for a reduction in the levels of total phenolic compounds. Considering the enhanced biometric and biochemical parameters and greater dry weight and leaf area of the seedlings grown with supplemental light, using such a tool can optimize seedling development, possibly reducing production time in the nursery and providing greater productivity.","PeriodicalId":508968,"journal":{"name":"Stresses","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139439165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Toxicological and Pharmacological Evaluation of the Anacyclus pyrethrum Aqueous Extract: Implications for Medicinal and Therapeutic Applications 除虫菊水提取物的毒理学和药理学评价：对药用和治疗应用的启示

Stresses Pub Date : 2024-01-09 DOI: 10.3390/stresses4010005

Abdelmounaim Baslam, R. Aboufatima, Hamid Kabdy, S. Boussaa, A. Chait, Marouane Baslam

{"title":"The Toxicological and Pharmacological Evaluation of the Anacyclus pyrethrum Aqueous Extract: Implications for Medicinal and Therapeutic Applications","authors":"Abdelmounaim Baslam, R. Aboufatima, Hamid Kabdy, S. Boussaa, A. Chait, Marouane Baslam","doi":"10.3390/stresses4010005","DOIUrl":"https://doi.org/10.3390/stresses4010005","url":null,"abstract":"Plants have long been valued for their medicinal and nutritional contributions to human life. Anacyclus pyrethrum, a member of the Asteraceae family, has attracted increasing attention as a source of natural products with diverse applications. In this study, we explored the toxicity and pharmacological properties of the aqueous extract of A. pyrethrum (AEAP). The acute toxicity study involved groups of mice subjected to oral administration of varying doses of AEAP, with immediate post-administration observations to detect any signs of toxicity or mortality. Comprehensive biochemical and hematological analyses encompassed assessments of renal function. The pharmacological profile was assessed by evaluating antinociceptive, anxiolytic, and antidepressant effects, which were measured using the hot plate test, elevated plus maze, open field test, and forced swim test, respectively. Different doses (100, 200, 400, and 800 mg/kg) were administered to rats via gavage for this assessment. The results revealed that the acute toxicity demonstrated the safety of AEAP at the tested doses, with no observed mortality or significant alterations. Moreover, it revealed that AEAP possesses an LD50 value greater than 5000 mg/kg. The pharmacological properties of AEAP demonstrated anxiolytic and antidepressant activities at a dose of 200 mg/kg, while no antinociceptive effect was observed. These findings underscore the potential of A. pyrethrum as a natural source of bioactive compounds with therapeutic applications. Further research is needed to explore long-term and chronic effects for a comprehensive assessment.","PeriodicalId":508968,"journal":{"name":"Stresses","volume":"17 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139443092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orbital Inflammation in Thyroid Eye Disease: Stress Responses and Their Implications 甲状腺眼病的眼眶炎症：应激反应及其影响

Stresses Pub Date : 2024-01-08 DOI: 10.3390/stresses4010004

Tracy Aoun, Diana Danielova Gueorguieva, Kevin Y. Wu

引用次数: 0

Role of Ethylene in the Regulation of Plant Developmental Processes 乙烯在植物发育过程调控中的作用

Stresses Pub Date : 2024-01-08 DOI: 10.3390/stresses4010003

Sheen Khan, Ameena Fatima Alvi, Nafees A. Khan

引用次数: 0

Functional and Qualitative Metabolic Compounds in the Twigs of the Deciduous Mistletoe Loranthus europaeus Jacq. 落叶槲寄生小枝中的功能性和定性代谢化合物 Loranthus europaeus Jacq.

Stresses Pub Date : 2023-12-29 DOI: 10.3390/stresses4010002

Anthi Bampali, O. Karoutzou, Alexandra Katsarou, K. Haralampidis, L. Skaltsounis, Sophia Rhizopoulou

{"title":"Functional and Qualitative Metabolic Compounds in the Twigs of the Deciduous Mistletoe Loranthus europaeus Jacq.","authors":"Anthi Bampali, O. Karoutzou, Alexandra Katsarou, K. Haralampidis, L. Skaltsounis, Sophia Rhizopoulou","doi":"10.3390/stresses4010002","DOIUrl":"https://doi.org/10.3390/stresses4010002","url":null,"abstract":"In this study, a detailed phytochemical investigation of compounds in the twigs of the mistletoe Loranthus europaeus Jacq., which belongs to the Loranthaceae family, is presented. Specimens were collected from the mistletoe L. europaeus growing on oak trees in the mainland of Greece. The alliance of oaks and mistletoes became a symbol of knowledge and strength for many centuries. Although numerous compounds of aerial tissues of other mistletoes, e.g., Viscum album, have been published, few studies have been conducted to investigate the metabolic and physiological traits of the hemiparasitic, deciduous Loranthus europaeus. LC-HRMS-based analysis led to a detailed characterization of ethyl acetate and dichloromethane extracts of the twigs of L. europaeus, which, to the best of our knowledge, exhibit enhanced antioxidant potential. Hence, twenty-four and twenty-six compounds were tentatively identified from the ethyl acetate and dichloromethane twigs’ extracts, respectively; these compounds belong to fatty acids, flavonoids, and flavonoid glycosides. Also, chlorophyll, soluble sugar, starch, and lipid contents in the twigs of L. europaeus, which have not hitherto been published, were investigated.","PeriodicalId":508968,"journal":{"name":"Stresses","volume":"8 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139147563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Binge-like Alcohol Administration Alters Decision Making in an Adolescent Rat Model: Role of N-Methyl-D-Aspartate Receptor Signaling 狂饮型酒精管理会改变青少年大鼠模型的决策：N-Methyl-D-Aspartate 受体信号的作用

Stresses Pub Date : 2023-12-22 DOI: 10.3390/stresses4010001

Camila Arce, Rodrigo G. Mira, Matías Lira, W. Cerpa

{"title":"Binge-like Alcohol Administration Alters Decision Making in an Adolescent Rat Model: Role of N-Methyl-D-Aspartate Receptor Signaling","authors":"Camila Arce, Rodrigo G. Mira, Matías Lira, W. Cerpa","doi":"10.3390/stresses4010001","DOIUrl":"https://doi.org/10.3390/stresses4010001","url":null,"abstract":"Alcohol is one of the most used legal drugs abused worldwide, and its consumption is associated with high mortality and morbidity rates. There is an increasing concern about the starting age of consumption of this drug since it has become evident that it is at younger ages. The so-called “pattern of consumption by binge” corresponds to ingesting large amounts of alcohol in a short period and is the most popular among young people. Previous studies show that alcohol causes damage in different areas, such as the hippocampus, hypothalamus, and prefrontal cortex, and adolescents are more susceptible to alcohol toxicity. Alcohol inhibits the membrane glutamate receptor, NMDA-type glutamate receptors (NMDAR). Using a binge-like alcohol administration protocol in adolescent rats (PND25), we investigate decision making through the attentional set-shifting test (ASST) and alterations in the NMDAR signaling in related areas. We observe an impairment in executive function without alterations in NMDAR abundance. However, binge alcohol changes NMDAR signaling and decreases quantity in the synapse, mainly in the hippocampus and hypothalamus. We suggest that prefrontal cortex impairment could arise from damaged connections with the hippocampus and hypothalamus, affecting the survival pathway and memory and learning process.","PeriodicalId":508968,"journal":{"name":"Stresses","volume":"28 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139166178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Stress Predictors in Vaquejada Horses Running with Different Interval Rest Periods 不同间歇休息时间下瓦克哈达马的压力预测分析

Stresses Pub Date : 2023-12-13 DOI: 10.3390/stresses3040058

C. Coelho, T. D. Sodré, Lara N. Sousa, Thadeu M. Silva, J. Simões, V. Souza, R. F. Siqueira, H. C. Manso Filho

{"title":"Analysis of Stress Predictors in Vaquejada Horses Running with Different Interval Rest Periods","authors":"C. Coelho, T. D. Sodré, Lara N. Sousa, Thadeu M. Silva, J. Simões, V. Souza, R. F. Siqueira, H. C. Manso Filho","doi":"10.3390/stresses3040058","DOIUrl":"https://doi.org/10.3390/stresses3040058","url":null,"abstract":"The purpose of this study was to assess the stress responses associated with vaquejada simulating tests (VSTs). Ten clinically healthy 8.9 ± 4.3-year-old Quarter horses, weighing 441.3 ± 25.0 kg, performed two VSTs five days apart. In the first VST (M1), animals ran three times with a 5-min rest between races; and in the second (M2), they ran with a 15-min rest between races. Horses were examined before (T0) and immediately after the third run (T1) and at 4 h (T2) of recovery. Stress biomarkers included heart rate (HR), heart rate variability (HRV), cortisol, and white blood cell count. All variables were analyzed by ANOVA and Tukey tests, considering p < 0.05. A significant reduction in cortisol was observed for pull (p = 0.0463) and helper (p = 0.0349) horses when they had a 15-min rest between races. The rMSSD and mean R-R values for helper horses were also significantly lower in M2. In conclusion, the 15-min rest interval proved to be better than the 5-min period for both categories of equine athletes used in vaquejada mainly for helper horses. A longer rest time between races allowed the organic recovery necessary for these animals to impose a greater applied physical effort load, which is a fact that guarantees good performance and well-being.","PeriodicalId":508968,"journal":{"name":"Stresses","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139180504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osmotic Stress Alleviation in Saccharomyces cerevisiae for High Ethanol Fermentations with Different Wort Substrates 利用不同麦芽汁基质进行高乙醇发酵的酿酒酵母中的渗透压缓解作用

Stresses Pub Date : 2023-11-29 DOI: 10.3390/stresses3040055

Rafael Douradinho, P. Sica, Fernando Tonoli, Eduardo Mattos, Matheus Oliveira, Alana Pinto, L. Mota, T. Faria, Vitória Franco Costa, Gabriela Leite, Valter Arthur, Suani Coelho, Antonio Baptista

{"title":"Osmotic Stress Alleviation in Saccharomyces cerevisiae for High Ethanol Fermentations with Different Wort Substrates","authors":"Rafael Douradinho, P. Sica, Fernando Tonoli, Eduardo Mattos, Matheus Oliveira, Alana Pinto, L. Mota, T. Faria, Vitória Franco Costa, Gabriela Leite, Valter Arthur, Suani Coelho, Antonio Baptista","doi":"10.3390/stresses3040055","DOIUrl":"https://doi.org/10.3390/stresses3040055","url":null,"abstract":"High-gravity fermentation, used for ethanol production from sugarcane, corn, and mixed substrates, offers several benefits. Yeast, a rapidly multiplying unicellular microorganism, can be adapted for high sugar and ethanol tolerance on a lab scale. However, different substrates can enhance fermentation efficiency. Our study consisted of two experiments. In the first, we compared simple batch feeding with a fed-batch system for yeast selection in high-gravity fermentation. We ran eight cycles with increasing initial sugar contents (50 to 300 g L−1). No significant differences were observed in the first seven cycles, but in the eighth, the fed-batch system showed lower glycerol and fructose contents and higher cell viability than the simple batch system. In the second experiment, we used the fed-batch system with 300 g L−1 from sugarcane, corn, and mixed wort. The results showed that mixed wort produced higher ethanol contents and greater fermentation efficiency compared to corn and sugarcane as substrates. In conclusion, our findings indicate that the fed-batch system is more suitable for high-gravity fermentation on a lab scale, and the combination of sugarcane juice and corn can enhance fermentation efficiency, paving the way for integrating these substrates in industrial ethanol production.","PeriodicalId":508968,"journal":{"name":"Stresses","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139212384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional Changes during Phytophthora capsici Infection Reveal Potential Defense Mechanisms in Squash 疫霉感染期间的转录变化揭示了南瓜的潜在防御机制

Stresses Pub Date : 2023-11-29 DOI: 10.3390/stresses3040056

Shailesh Raj Acharya, Swati Shrestha, V. Michael, Yuqing Fu, Prerna Sabharwal, Shallu Thakur, G. Meru

{"title":"Transcriptional Changes during Phytophthora capsici Infection Reveal Potential Defense Mechanisms in Squash","authors":"Shailesh Raj Acharya, Swati Shrestha, V. Michael, Yuqing Fu, Prerna Sabharwal, Shallu Thakur, G. Meru","doi":"10.3390/stresses3040056","DOIUrl":"https://doi.org/10.3390/stresses3040056","url":null,"abstract":"Phytophthora capsici incites foliar blight, root, fruit, and crown rot in squash (Cucurbita spp.) and limits production worldwide. Resistance to crown rot in C. moschata breeding line #394-1-27-12 is conferred by three dominant genes, but the molecular mechanisms underlying this resistance are poorly understood. In the current study, RNA sequencing was used to investigate transcriptional changes in #394-1-27-12 (resistant) and Butterbush (susceptible) following infection by P. capsici at 12, 24, 48, 72, and 120 h post inoculation (hpi). Overall, the number of differentially expressed genes (DEGs) in Butterbush (2648) exceeded those in #394-1-27-12 (1729), but in both genotypes, the highest number of DEGs was observed at 72 hpi and least at 24 hpi. Our gene ontology (GO) analysis revealed a downregulation of the genes involved in polysaccharide and lignin metabolism in Butterbush but as an upregulation of those associated with regulation of peptidase activity. However, in #394-1-27-12, the downregulated genes were primarily associated with response to stimuli, whereas those upregulated were involved in oxidation–reduction and response to stress. The upregulated genes in #394-1-27-12 included defensin-like proteins, respiratory-burst oxidases, ethylene-responsive transcription factors, cytochrome P450 proteins, and peroxidases. These findings provide a framework for the functional validation of the molecular mechanisms underlying resistance to P. capsici in cucurbits.","PeriodicalId":508968,"journal":{"name":"Stresses","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139214941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0