Transcriptional Changes during Phytophthora capsici Infection Reveal Potential Defense Mechanisms in Squash

Stresses Pub Date : 2023-11-29 DOI:10.3390/stresses3040056
Shailesh Raj Acharya, Swati Shrestha, V. Michael, Yuqing Fu, Prerna Sabharwal, Shallu Thakur, G. Meru
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引用次数: 0

Abstract

Phytophthora capsici incites foliar blight, root, fruit, and crown rot in squash (Cucurbita spp.) and limits production worldwide. Resistance to crown rot in C. moschata breeding line #394-1-27-12 is conferred by three dominant genes, but the molecular mechanisms underlying this resistance are poorly understood. In the current study, RNA sequencing was used to investigate transcriptional changes in #394-1-27-12 (resistant) and Butterbush (susceptible) following infection by P. capsici at 12, 24, 48, 72, and 120 h post inoculation (hpi). Overall, the number of differentially expressed genes (DEGs) in Butterbush (2648) exceeded those in #394-1-27-12 (1729), but in both genotypes, the highest number of DEGs was observed at 72 hpi and least at 24 hpi. Our gene ontology (GO) analysis revealed a downregulation of the genes involved in polysaccharide and lignin metabolism in Butterbush but as an upregulation of those associated with regulation of peptidase activity. However, in #394-1-27-12, the downregulated genes were primarily associated with response to stimuli, whereas those upregulated were involved in oxidation–reduction and response to stress. The upregulated genes in #394-1-27-12 included defensin-like proteins, respiratory-burst oxidases, ethylene-responsive transcription factors, cytochrome P450 proteins, and peroxidases. These findings provide a framework for the functional validation of the molecular mechanisms underlying resistance to P. capsici in cucurbits.
疫霉感染期间的转录变化揭示了南瓜的潜在防御机制
疫霉菌(Phytophthora capsici)会导致南瓜(Cucurbita spp.)的叶枯病、根腐病、果腐病和冠腐病,并在全球范围内限制产量。C. moschata育种品系#394-1-27-12对冠腐病的抗性是由三个显性基因赋予的,但人们对这种抗性的分子机制知之甚少。本研究利用 RNA 测序技术研究了 #394-1-27-12(抗性)和 Butterbush(易感性)在接种后 12、24、48、72 和 120 小时受到荚膜褐斑病菌感染后的转录变化。总体而言,黄油树的差异表达基因(DEGs)数量(2648 个)超过了 #394-1-27-12(1729 个),但在这两种基因型中,72 hpi 时的差异表达基因数量最多,24 hpi 时最少。我们的基因本体(GO)分析表明,在黄油树中,参与多糖和木质素代谢的基因下调,但与肽酶活性调节有关的基因上调。不过,在#394-1-27-12 中,下调的基因主要与对刺激的反应有关,而上调的基因则与氧化还原和对压力的反应有关。#394-1-27-12中上调的基因包括防御素样蛋白、呼吸猝灭氧化酶、乙烯反应转录因子、细胞色素P450蛋白和过氧化物酶。这些发现为从功能上验证葫芦对荚膜锈菌抗性的分子机制提供了一个框架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
4.70
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