Pharmacia最新文献

{"title":"3’-Methyl-4-thio-1H-tetrahydropyranspiro-5’-hydantoin platinum complex as a novel deoxyribonuclease I inhibitor","authors":"Ana Marković, M. Atanasova, R. Buyukliev, A. Bakalova, A. Šmelcerović, E. Cherneva","doi":"10.3897/pharmacia.71.e126246","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e126246","url":null,"abstract":"Deoxyribonuclease I (DNase I) is one of the main nucleases involved in deoxyribonucleic acid (DNA) degradation during apoptosis. It catalyzes the hydrolytic cleavage of DNA, producing 5‘-oligonucleotides. The inhibition of DNase I may serve as an important mechanism for protecting DNA against premature degradation during cell damage. Fourteen hydantoin-containing compounds, including two newly synthesized and seven previously synthesized metal complexes, along with five previously synthesized hydantoin ligands, were evaluated in vitro for their inhibitory properties against bovine pancreatic DNase I. As a result, the 3’-methyl-4-thio-1H-tetrahydropyranspiro-5’-hydantoin platinum complex (8) inhibited the enzyme with an IC50 value of 110.20 ± 24.20 µM, a potency 3-fold greater than that of the reference crystal violet (IC50 = 378.27 ± 47.75 µM). To understand the binding mode and mechanism of inhibition of compound 8 with DNase I, molecular docking calculations were performed. The analysis revealed that compound 8 interacts with the most important catalytic residues of DNase I. To the best of our knowledge, this is the first report of a platinum complex inhibiting DNase I.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"41 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141683195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proton therapy for head and neck cancer therapy: A real-world data case study from Bulgaria","authors":"Maria Dimitrova, Nezabravka Tsvetanova, Daniel Penchev, G. Petrova","doi":"10.3897/pharmacia.71.e129379","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e129379","url":null,"abstract":"Head and neck cancers (HNC) are one of the most severe types of cancer in Europe, accounting for almost 4.5% of all cancer types, according to GLOBOCAN. The incidence is geographically variable, with higher rates observed in Eastern and Southern Europe than in Northern and Western Europe. Proton and photon therapies are both available treatment approaches for head and neck cancer, as each has its own unique advantages, characteristics, and considerations. Proton therapy is the newest one and is considered safer among both. The current study aims to analyse the available data for patients with head and neck cancer treated with proton therapy in Bulgaria based on real-world data generated through AI-based patient registries and the current availability of strategic policy documents ensuring the affordability of proton therapy on a national level. We wanted to explore the feasibility of building a national proton therapy centre based on available patients’ information and strategic policy documents. We conducted a 3-year (2020–2022) combined non-interventional retrospective database study on head and neck cancer using secondary use of real-world data from dynamic patient registries and desktop analysis of strategic policy documents for capacity and financial affordability for proton therapy in Bulgaria. The results show that Bulgaria has a strategic policy document that focuses on the need and funding possibilities for establishing this treatment within the country. However, the country lacks the political will to ensure appropriate funding for it. Building a national proton centre is a feasible investment but needs additional detailed budget impact analysis.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"7 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141685750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical fingerprint analysis for quality assessment and control of Curcuma longa L. rhizomes from Vietnam using a high-performance liquid chromatography-diode array detector (HPLC-DAD)","authors":"Tho Do Chau Minh Vinh, Sil Nguyen Thanh, Ngan Nguyen Thanh, Tham Le Kim, Thi Huynh Huynh Anh, Giang Le Thi Truc, Mai Nguyen Thi Hong","doi":"10.3897/pharmacia.71.e124050","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e124050","url":null,"abstract":"Turmeric, extensively cultivated across Southeast Asia, especially in Vietnam, harbors active polyphenols, primarily curcumin (2–5%), renowned for its diverse health benefits. Pharmacopoeias recognize turmeric, yet it lacks standardized quality assessments and encounters challenges in extraction and identification due to natural variations and adulteration. This analytical method is vital for verifying the authenticity, purity, and quality of turmeric products in both the pharmaceutical and nutraceutical industries. This study successfully developed an efficient extraction process for curcumin (CUR), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) from Curcuma longa L. rhizomes. The herbal powder was extracted with methanol (1:30, w/v) by the ultrasound-assisted method for 10 minutes, and this process was repeated three times. A high-performance liquid chromatography-diode array detector (HPLC-DAD) method was validated for the simultaneous quantification of three analytes, following the AOAC guideline and achieving a correlation coefficient (R2) value greater than 0.9950. Utilizing the HPLC-DAD method, the study developed a chemical fingerprint analysis for three analytes to identify the characteristic chemical components distinguishing turmeric from each region. Nineteen samples collected from various provinces across Vietnam were subjected to analysis. In all analyzed samples, the concentrations of CUR, DMC, and BDMC ranged from 0.77–10.30%, 0.33–6.92%, and 0.03–3.23%, respectively. CUR was determined to be the dominant compound in most samples, while BDMC consistently exhibited the lowest levels of content. Utilizing the findings derived from the analysis of RRT and RPA metrics, the research assessed variances across sample batches. It is suggested that this newly established approach can be applied to construct and develop raw material areas to serve the needs of each field.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"359 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141686426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of the FTIR spectroscopic method for the quantitative determination of the narrow therapeutic index levothyroxine sodium in pharmaceutical tablets","authors":"Malak AlBathish, A. Gazy, Marwa Al Jamal, A. Bejjani","doi":"10.3897/pharmacia.71.e125879","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e125879","url":null,"abstract":"Levothyroxine sodium is a narrow therapeutic index drug used for the treatment of hypothyroidism. The medication is marketed in tablet form with very low doses ranging from 25 to 150 µg, which requires the development of a sensitive quantitative analytical method to ensure a safe and effective pharmacological response. In the present work, a Fourier transform infrared method has been developed and validated for levothyroxine sodium determination in various pharmaceutical formulations. The proposed method involves selectively extracting levothyroxine sodium from the studied tablets using chloroform as solvent, then depositing it on a KBr pellet, followed by infrared measurements and spectra analysis. The peak band area corresponding to the C=C centered at 1409 cm-1 has been chosen for the quantification. The method has been validated according to ICH guidelines and was found to be simple, precise, accurate, and specific. The linearity, detection, and quantitation limits are 25–800, 8.121, and 24.545 µg/pellet, respectively. These values confer the method’s sensitivity and applicability for the determination of different pharmaceutical tablets with various dosages. A statistical comparison with a reference HPLC method showed no significant difference. Accordingly, the developed method can be employed for quality control testing of levothyroxine sodium due to its simplicity and the absence of sophisticated instrumentation and procedures.\u0000 Graphical abstract:\u0000 \u0000 \u0000","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"14 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141700578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug interaction detection and glycemic control – telepharmacy’s role in diabetes management: before-after study","authors":"Ida Lisni, Irma Rahayu Latarissa, Lucia Rizka Andalusia, Keri Lestari","doi":"10.3897/pharmacia.71.e123313","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e123313","url":null,"abstract":"Introduction: Diabetes management faces intertwined challenges like polypharmacy and adherence complexities, hindering effective care delivery. Telepharmacy emerges as a promising solution, revolutionizing diabetes care by enhancing pharmacists’ pivotal role. However, a tailored model for Indonesia is still lacking.\u0000 Purpose: This study aimed to assess the potential effectiveness of a telepharmacy application in improving the detection of drug-drug interactions and glycemic control.\u0000 Methods: We conducted an observational before-and-after study among type 2 diabetic patients in Indonesia.\u0000 Results: We noted a significant surge in detected drug interactions. Patients counseled through telepharmacy had 3.653 times higher likelihood of achieving good glycemic control.\u0000 Conclusion: The study underscores the positive impact of telepharmacy on drug interaction detection and glycemic control among Indonesian diabetics.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"56 45","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141102993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of dietary intervention on myokines: a narrative review","authors":"Yavor Assyov, Kristian Chechev, Antoaneta Gateva, Karamfilova Vera, Tsvetan Gatev, Dobrin Popov, Yuliyan Naydenov, Emanuela Zaharieva, Natalia Konova, Savelia Yordanova, Bozhidar Marinov, Diana Nikolova, Zdravko Kamenov","doi":"10.3897/pharmacia.71.e123805","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e123805","url":null,"abstract":"This review elucidates the role of dietary factors in influencing myokine secretion, complementing the recognized benefits of physical activity on metabolic health. While exercise is known to positively modulate myokines, contributing to their health-promoting effects, an exploration into how diet affects these critical cytokines remains sparse. Through analysis of the literature, this work identifies dietary patterns that markedly affect myokine levels, underscoring the complex relationship between nutrition and myokine activity. The investigation reveals that specific dietary approaches can profoundly alter myokine secretion, suggesting an essential avenue for combined dietary and exercise interventions to optimize health outcomes. These insights highlight the need for further detailed study into diet-induced myokine modulation.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"72 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141106041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and application of gold nanoparticles as antioxidants","authors":"B. A. Suliasih, Setia Budi, Haliza Katas","doi":"10.3897/pharmacia.71.e112322","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e112322","url":null,"abstract":"Non-communicable diseases (NCDs) and premature aging, caused by free radicals, have spurred a demand for extensive research into finding effective antioxidants. Currently, there is an abundance of both natural and synthetic antioxidants, including metal nanoparticles with high antioxidant activity. Among these, gold nanoparticles (AuNPs) stand out as favoured antioxidants because of their minimal toxicity, simple synthesis, and detectability. The antioxidant properties of AuNPs enhance its wide-ranging potential for use in healthcare including applications as anti-aging, anti-inflammatory, and wound healing agents, as well as treatment for various diseases. This review highlights recent progress in the synthesis of AuNPs as antioxidants and method for assessing their antioxidant capacity as well as delves into their mechanism of action and explores their potential health applications. In conclusion, considering the physicochemical and biological properties, along with the benefits and potential challenges for future development, AuNPs are deemed promising and effective antioxidants suitable for clinical applications.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141105540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of biological medicinal products and tyrosine kinase inhibitors in rheumatoid arthritis and COVID-19: A systematic review of scientific literature","authors":"I. Parvova, E. Yordanov, E. Hristov, V. Petkova","doi":"10.3897/pharmacia.71.e124471","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e124471","url":null,"abstract":"We analyzed whether commonly used biological medicinal products and/or tyrosine kinase inhibitors with indications for treatment of rheumatologic diseases can be used in patients with concurrent COVID-19 by systematically reviewing scientific articles. The articles were selected according to PRISMA by keywords in the MEDLINE and Central Medical Library - MU Sofia databases for the period January 2020–December 2023. We found 168 scientific publications, of which 9 met the set criteria. Results were evaluated using descriptive methods and the PICOS tool. We found no evidence that the use of BMPs and TKIs in patients with rheumatologic diseases leads to an increased risk of COVID-19 infection and/or a more severe course of SARS-CoV-2 infection. Several cases of prolonged or atypical COVID-19-induced pneumonia have been identified in patients treated with Rituximab. This suggests the need for caution in the use of this medicinal product in patients with COVID-19. The 2022 EULAR recommendations are in the same spirit.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"54 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141109182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of CYP2C19 genotypes on steady-state plasma concentration of escitalopram in South Indian population with Major Depressive Disorder","authors":"B. Jeevan Kumar, Vijayakumar Thangavel Mahalingam, Ganesh Kumar M","doi":"10.3897/pharmacia.71.e123645","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e123645","url":null,"abstract":"Background: Among depressed patients, escitalopram plasma levels differed between those who were considered as extensive metabolizers and poor metabolizers of CYP2C19. However, the majority of research utilized the dose-response relationship. Consequently, we investigated the effect of variations in the CYP2C19 gene on the levels of drug in the bloodstream of individuals suffering from Major Depressive Disorder (MDD) in south India.\u0000 Methods: A total of 109 individuals with MDD who were prescribed escitalopram at doses of 5, 10, 15, or 20 mg daily participated in this research. We used HPLC with SPD-10AVP UV-Visible detector to measure the escitalopram concentrations in the blood. The polymorphisms of the CYP2C19 were determined by employing the PCR techniques.\u0000 Results: Our study found that 55% of the subjects were intermediate metabolizers, followed by extensive (19.3%), poor (17.4%), and ultra-rapid (8.3%). The significant correlation is identified between the steady state plasma concentration and Sex with (P - Value < 0.05), insignificant correlation was seen in Age and BMI with (P - Value > 0.05). The majority of gene variants seen in the study population were CYP2C19*1/*2, accounting for 49 individuals (44.9%).\u0000 Conclusion: These findings showed that sex had a substantial impact on the CYP2C19 genetic variation. Medication administration to individuals with CYP2C19PM requires special caution.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"50 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141113208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological prophylaxis and functional restoration of the lower limb in total knee arthroprosthesis","authors":"Mariya Vakrilova Becheva, A. Kirkova-Bogdanova, Stefka Achkova Ivanova, Krasimira Milcheva Kazalakova","doi":"10.3897/pharmacia.71.e124586","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e124586","url":null,"abstract":"The complexity of the movements of the bones of the knee joint requires an anatomical study to construct a knee prosthesis. The reasons for knee replacement are related to wear of the articular cartilage, which leads to pain, deformation, and limitation of movements. The consequences of the surgical intervention allow functional restoration of the knee joint and the supporting function of the lower limb. Kinesitherapy begins the day after surgery and aims to reduce pain, restore mobility of the knee joint, actively lock the knee during extension, increase and maintain muscle strength, and ensure the functional independence of patients. To a large extent, the volume of movement is restored, and the pain symptoms are reduced. Patients have the comfort of everyday movements and the possibility of participating in certain sports activities—swimming, cycling, and golf. The paper deals with pharmacological prophylaxis, the kinesitherapeutic program, and total knee arthroprosthesis.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"37 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141122007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}