Advances in Parasitology最新文献

Advances, challenges, and applications of laboratory culture of digenean trematode parasites. 地沟吸虫实验室培养的进展、挑战和应用。

3区医学

Advances in Parasitology Pub Date : 2025-01-01 Epub Date: 2025-05-15 DOI: 10.1016/bs.apar.2025.04.001

Simão Correia, Sergio Fernández-Boo, Manuel Vera, Robert Poulin, Luísa Magalhães

{"title":"Advances, challenges, and applications of laboratory culture of digenean trematode parasites.","authors":"Simão Correia, Sergio Fernández-Boo, Manuel Vera, Robert Poulin, Luísa Magalhães","doi":"10.1016/bs.apar.2025.04.001","DOIUrl":"https://doi.org/10.1016/bs.apar.2025.04.001","url":null,"abstract":"The development of parasite cultures has long been pivotal in advancing parasitology, with broad applications in medicine, veterinary science, and biology. Laboratory cultures are invaluable tools for studying parasite biology, host-parasite interactions, and the development of treatments and vaccines. However, cultures of digenean trematodes under laboratory conditions remain a challenging yet critical endeavour in parasitology. These parasites hold significant importance to both human health and ecological systems. Nevertheless, trematodes exhibit a complex life cycle involving multiple hosts, which demands innovative culture methods. This review aims to provide a comprehensive overview of laboratory culture techniques for digenean trematodes, covering in vitro, in vivo, and in ovo approaches. These techniques are discussed in order across the different parasitic life stages of trematodes, from sporocyst/redia to adults, as well as the handling of trematode eggs, with a focus on optimising media composition, host-cell integration, and environmental parameters. In vitro approaches, particularly those using host-cell-based media or host-derived sera, have shown promise for certain zoonotic species. However, cultures of marine trematodes often face limitations due to suboptimal media protocols. On the other hand, in vivo and in ovo methods, while generally achieving higher success rates, raise ethical and logistical concerns. Despite notable progress, the standardisation of protocols and the adaptation of techniques for a broader range of species remain significant challenges in digenean trematode cultures. Future research should prioritise the development of host-cell-based media, innovative culture technologies, and integrative molecular and proteomic tools to address these limitations and further our understanding of trematode biology.","PeriodicalId":50854,"journal":{"name":"Advances in Parasitology","volume":"127 ","pages":"119-151"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144530929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acarofauna of Neotropical reptiles: Integrative morphology and vector competence of zoonotic pathogens. 新热带爬行动物：人畜共患病原体的整体形态和媒介能力。

3区医学

Advances in Parasitology Pub Date : 2025-01-01 Epub Date: 2025-05-24 DOI: 10.1016/bs.apar.2025.04.002

Jairo Alfonso Mendoza-Roldan, Marcos Antônio Bezerra-Santos, Darci Moraes Barros Battesti, Domenico Otranto

引用次数: 0

Cystoisospora suis - the non-model model coccidium. 猪囊异孢子虫——非模型模型球虫。

3区医学

Advances in Parasitology Pub Date : 2025-01-01 Epub Date: 2025-04-16 DOI: 10.1016/bs.apar.2025.03.001

Anja Joachim, Anna Feix, Bärbel Ruttkowski, Teresa Cruz-Bustos

{"title":"Cystoisospora suis - the non-model model coccidium.","authors":"Anja Joachim, Anna Feix, Bärbel Ruttkowski, Teresa Cruz-Bustos","doi":"10.1016/bs.apar.2025.03.001","DOIUrl":"https://doi.org/10.1016/bs.apar.2025.03.001","url":null,"abstract":"Cystoisospora suis, the cause of suckling piglet coccidiosis, is an intestinal protozoan pathogen of worldwide distribution and major economic and animal health significance in swine industry. It is closely related to cyst-forming, facultatively heteroxenic Coccidia like Toxoplasma gondii and Neospora caninum, but its biology resembles more that of the non-cyst-forming, homoxenic genus Eimeria. Lately, a unique in vitro cultivation system for C. suis was developed by which sporozoites infect monolayer cell cultures to produce merozoites which can in turn be propagated in a host-cell free system and develop into sexually differentiated gamonts, gametes and finally oocysts. This system has been used to produce and analyse developmental stages throughout the life cycle of C. suis. Transcriptomic, proteomic and secretomic data are now available, providing information for fundamental and applied research not only on this coccidian species but extrapolation to related parasites. In addition, antiparasitic compounds can be tested in this in vitro model, and further upscaling will provide a higher-throughput system for (pre-clinical) compound screening and in vitro efficacy testing for anticoccidial drugs, supporting the early detection of anticoccidial resistance in C. suis field strains. With these developments, C. suis can be considered a \"non-model model\" for the Coccidia, bridging the gap between the cyst-forming Sarcocystidae and the non-cyst-forming Eimeriidae, and between parasites of One Health relevance, such as T. gondii, and those members of the Coccidia that are of relevance in veterinary medicine and animal health.","PeriodicalId":50854,"journal":{"name":"Advances in Parasitology","volume":"127 ","pages":"1-25"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144530950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The nematode family Cloacinidae (Strongyloidea), parasites of Australasian kangaroos, wallabies and wombats: from morphology and ecology to molecules. 线虫科（圆线虫总科），澳大利亚袋鼠、小袋鼠和袋熊的寄生虫：从形态学、生态学到分子。

3区医学

Advances in Parasitology Pub Date : 2025-01-01 Epub Date: 2025-05-12 DOI: 10.1016/bs.apar.2025.03.002

Tanapan Sukee, Ian Beveridge, Anson V Koehler, Robin B Gasser, Abdul Jabbar

引用次数: 0

An update and review of arthropod vector sensory systems: Potential targets for behavioural manipulation by parasites and other disease agents. 节肢动物媒介感觉系统的更新与回顾：寄生虫和其他病原体操纵行为的潜在目标。

3区医学

Advances in Parasitology Pub Date : 2024-01-01 Epub Date: 2024-03-12 DOI: 10.1016/bs.apar.2024.02.003

Jean-François Doherty, Tahnee Ames, Leisl Imani Brewster, Jonathan Chiang, Elsa Cyr, Cameron R Kelsey, Jeehan Phillip Lee, Bingzong Liu, Ivan Hok Yin Lo, Gurleen K Nirwal, Yunusa Garba Mohammed, Orna Phelan, Parsa Seyfourian, Danica Marie Shannon, Nicholas Kristoff Tochor, Benjamin John Matthews

{"title":"An update and review of arthropod vector sensory systems: Potential targets for behavioural manipulation by parasites and other disease agents.","authors":"Jean-François Doherty, Tahnee Ames, Leisl Imani Brewster, Jonathan Chiang, Elsa Cyr, Cameron R Kelsey, Jeehan Phillip Lee, Bingzong Liu, Ivan Hok Yin Lo, Gurleen K Nirwal, Yunusa Garba Mohammed, Orna Phelan, Parsa Seyfourian, Danica Marie Shannon, Nicholas Kristoff Tochor, Benjamin John Matthews","doi":"10.1016/bs.apar.2024.02.003","DOIUrl":"10.1016/bs.apar.2024.02.003","url":null,"abstract":"For over a century, vector ecology has been a mainstay of vector-borne disease control. Much of this research has focused on the sensory ecology of blood-feeding arthropods (black flies, mosquitoes, ticks, etc.) with terrestrial vertebrate hosts. Of particular interest are the cues and sensory systems that drive host seeking and host feeding behaviours as they are critical for a vector to locate and feed from a host. An important yet overlooked component of arthropod vector ecology are the phenotypic changes observed in infected vectors that increase disease transmission. While our fundamental understanding of sensory mechanisms in disease vectors has drastically increased due to recent advances in genome engineering, for example, the advent of CRISPR-Cas9, and high-throughput \"big data\" approaches (genomics, proteomics, transcriptomics, etc.), we still do not know if and how parasites manipulate vector behaviour. Here, we review the latest research on arthropod vector sensory systems and propose key mechanisms that disease agents may alter to increase transmission.","PeriodicalId":50854,"journal":{"name":"Advances in Parasitology","volume":"124 ","pages":"57-89"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Getting around the roundworms: Identifying knowledge gaps and research priorities for the ascarids. 绕过蛔虫：确定蛔虫的知识差距和研究重点。

3区医学

Advances in Parasitology Pub Date : 2024-01-01 Epub Date: 2024-02-20 DOI: 10.1016/bs.apar.2023.12.002

Adrian J Wolstenholme, Erik C Andersen, Shivani Choudhary, Friederike Ebner, Susanne Hartmann, Lindy Holden-Dye, Sudhanva S Kashyap, Jürgen Krücken, Richard J Martin, Ankur Midha, Peter Nejsum, Cedric Neveu, Alan P Robertson, Georg von Samson-Himmelstjerna, Robert Walker, Jianbin Wang, Bradley J Whitehead, Paul D E Williams

{"title":"Getting around the roundworms: Identifying knowledge gaps and research priorities for the ascarids.","authors":"Adrian J Wolstenholme, Erik C Andersen, Shivani Choudhary, Friederike Ebner, Susanne Hartmann, Lindy Holden-Dye, Sudhanva S Kashyap, Jürgen Krücken, Richard J Martin, Ankur Midha, Peter Nejsum, Cedric Neveu, Alan P Robertson, Georg von Samson-Himmelstjerna, Robert Walker, Jianbin Wang, Bradley J Whitehead, Paul D E Williams","doi":"10.1016/bs.apar.2023.12.002","DOIUrl":"10.1016/bs.apar.2023.12.002","url":null,"abstract":"The ascarids are a large group of parasitic nematodes that infect a wide range of animal species. In humans, they cause neglected diseases of poverty; many animal parasites also cause zoonotic infections in people. Control measures include hygiene and anthelmintic treatments, but they are not always appropriate or effective and this creates a continuing need to search for better ways to reduce the human, welfare and economic costs of these infections. To this end, Le Studium Institute of Advanced Studies organized a two-day conference to identify major gaps in our understanding of ascarid parasites with a view to setting research priorities that would allow for improved control. The participants identified several key areas for future focus, comprising of advances in genomic analysis and the use of model organisms, especially Caenorhabditis elegans, a more thorough appreciation of the complexity of host-parasite (and parasite-parasite) communications, a search for novel anthelmintic drugs and the development of effective vaccines. The participants agreed to try and maintain informal links in the future that could form the basis for collaborative projects, and to co-operate to organize future meetings and workshops to promote ascarid research.","PeriodicalId":50854,"journal":{"name":"Advances in Parasitology","volume":"123 ","pages":"51-123"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140050955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in protease inhibition-based chemotherapy: A decade of insights from Malaria research. 基于蛋白酶抑制剂的化疗进展：疟疾研究的十年启示。

3区医学

Advances in Parasitology Pub Date : 2024-01-01 Epub Date: 2024-08-30 DOI: 10.1016/bs.apar.2024.07.001

Daniel Sojka, Pavla Šnebergerová

{"title":"Advances in protease inhibition-based chemotherapy: A decade of insights from Malaria research.","authors":"Daniel Sojka, Pavla Šnebergerová","doi":"10.1016/bs.apar.2024.07.001","DOIUrl":"https://doi.org/10.1016/bs.apar.2024.07.001","url":null,"abstract":"Over the last decade, research on the most studied parasite, Plasmodium falciparum, has disclosed significant findings in protease research. Detailed descriptions of the individual roles of protease isoenzymes from various protease classes encoded by the parasite genome have been elucidated, along with their functional and biochemical characterizations. These insights have enabled the development of innovative chemotherapy using low molecular weight inhibitors targeting specific molecular sites. Progress has been made in understanding the proteolytic cascade associated with the apical complex, particularly the roles of aspartyl proteases plasmepsins IX and X as master regulators. Additionally, advancements in direct and alternative methods of proteasome inhibition and expression regulation have been achieved. Research on digestive/food vacuole-associated proteases, with a focus on essential metalloproteases, has also seen significant developments. The rise of extensive genomic datasets and functional genomic tools for other parasitic organisms now allows these approaches to be applied to the study and treatment of other, less known parasitic diseases, aiming to uncover specific biological mechanisms and develop innovative, less toxic chemotherapies.","PeriodicalId":50854,"journal":{"name":"Advances in Parasitology","volume":"126 ","pages":"205-227"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astacin metalloproteases in human-parasitic nematodes. 人类寄生线虫中的天星素金属蛋白酶

3区医学

Advances in Parasitology Pub Date : 2024-01-01 Epub Date: 2024-04-14 DOI: 10.1016/bs.apar.2024.03.001

Matthew S Moser, Elissa A Hallem

{"title":"Astacin metalloproteases in human-parasitic nematodes.","authors":"Matthew S Moser, Elissa A Hallem","doi":"10.1016/bs.apar.2024.03.001","DOIUrl":"https://doi.org/10.1016/bs.apar.2024.03.001","url":null,"abstract":"Parasitic nematodes infect over 2 billion individuals worldwide, primarily in low-resource areas, and are responsible for several chronic and potentially deadly diseases. Throughout their life cycle, these parasites are thought to use astacin metalloproteases, a subfamily of zinc-containing metalloendopeptidases, for processes such as skin penetration, molting, and tissue migration. Here, we review the known functions of astacins in human-infective, soil-transmitted parasitic nematodes - including the hookworms Necator americanus and Ancylostoma duodenale, the threadworm Strongyloides stercoralis, the giant roundworm Ascaris lumbricoides, and the whipworm Trichuris trichiura - as well as the human-infective, vector-borne filarial nematodes Wuchereria bancrofti, Onchocerca volvulus, and Brugia malayi. We also review astacin function in parasitic nematodes that infect other mammalian hosts and discuss the potential of astacins as anthelmintic drug targets. Finally, we highlight the molecular and genetic tools that are now available for further exploration of astacin function and discuss how a better understanding of astacin function in human-parasitic nematodes could lead to new avenues for nematode control and drug therapies.","PeriodicalId":50854,"journal":{"name":"Advances in Parasitology","volume":"126 ","pages":"177-204"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmodium proteases and their role in development of Malaria vaccines. 疟原虫蛋白酶及其在开发疟疾疫苗中的作用。

3区医学

Advances in Parasitology Pub Date : 2024-01-01 Epub Date: 2024-09-25 DOI: 10.1016/bs.apar.2024.08.001

Gunjan Arora, Jiří Černý

引用次数: 0

Immunology and pathology of echinostomes and other intestinal trematodes. 棘尾虫和其他肠道吸虫的免疫学和病理学。

3区医学

Advances in Parasitology Pub Date : 2024-01-01 Epub Date: 2024-03-08 DOI: 10.1016/bs.apar.2024.02.002

Rafael Toledo, Paola Cociancic, Emma Fiallos, J Guillermo Esteban, Carla Muñoz-Antoli

{"title":"Immunology and pathology of echinostomes and other intestinal trematodes.","authors":"Rafael Toledo, Paola Cociancic, Emma Fiallos, J Guillermo Esteban, Carla Muñoz-Antoli","doi":"10.1016/bs.apar.2024.02.002","DOIUrl":"10.1016/bs.apar.2024.02.002","url":null,"abstract":"Intestinal trematodes constitute a major group of helminths that parasitize humans and animals with relevant morbidity and mortality. Despite the importance of the intestinal trematodes in medical and veterinary sciences, immunology and pathology of these helminth infections have been neglected for years. Apart from the work focused on the members of the family Echnistomatidae, there are only very isolated and sporadic studies on the representatives of other families of digeneans, which makes a compilation of all these studies necessary. In the present review, the most salient literature on the immunology and pathology of intestinal trematodes in their definitive hosts in examined. Emphasis will be placed on members of the echinostomatidae family, since it is the group in which the most work has been carried out. However, we also review the information on selected species of the families Brachylaimidae, Diplostomidae, Gymnophallidae, and Heterophyidae. For most of these families, coverage is considered under the following headings: (i) Background; (ii) Pathology of the infection; (iii) Immunology of the infection; and (iv) Human infections.","PeriodicalId":50854,"journal":{"name":"Advances in Parasitology","volume":"124 ","pages":"1-55"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0