SEPARATION SCIENCE PLUS最新文献_第2页

Advancements in solvent microextraction: Recent developments and diverse applications in the modern era 溶剂微萃取的进展：当代的最新发展和多样化应用

SEPARATION SCIENCE PLUS Pub Date : 2024-03-03 DOI: 10.1002/sscp.202300243

Priyanka Rani, Bibhu Prasad Nanda, R. Narang, Rohit Bhatia

{"title":"Advancements in solvent microextraction: Recent developments and diverse applications in the modern era","authors":"Priyanka Rani, Bibhu Prasad Nanda, R. Narang, Rohit Bhatia","doi":"10.1002/sscp.202300243","DOIUrl":"https://doi.org/10.1002/sscp.202300243","url":null,"abstract":"Solvent microextraction (SME) is designed to selectively isolate desired analytes while minimizing the presence of interfering substances in the extracted sample. There are different approaches from which single‐drop microextraction (SDME) can be amalgamated with various analytical methods to enhance its functionalities and improve the analysis of extracted compounds like gas chromatography, and liquid chromatography amplify sensitivity, selectivity, and the capacity to detect trace amounts of analytes in complex matrices. The review focuses on the intricacies of solid‐phase microextraction and elucidates its operational principles. Additionally, it explores various types of SDMEs, detailing their respective benefits, drawbacks, and potential applications in the future. A typical explanation of the construction and working of hollow fiber‐liquid phase microextraction as well as its types has been discussed. The applications of SMEs in the medical and biomedical fields from food analysis to environmental analysis like analysis and purification of water, organic pollutants, drug analysis, and screening of new drugs have been discussed and also illustrated in tabular form. The pros and cons of both analytical techniques are given in a tabular manner as well a broad comparison between different types of advanced SME techniques provides a new direction for environmental monitoring, food safety, and refining of impurities from water samples. The review provides a comprehensive overview of the current progress, recent obstacles, and future possibilities of SMEs in an appropriate manner. Despite numerous promising advancements, the utilization of SMEs remains a challenging field.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140081225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent overview of microextraction of metal ions and pharmaceuticals by solidified floating organic drop microextraction techniques 利用固化浮动有机液滴微萃取技术微萃取金属离子和药物的最新综述

SEPARATION SCIENCE PLUS Pub Date : 2024-03-03 DOI: 10.1002/sscp.202400018

Shivam Kumar, Sourabh Satapathy, B. Kurmi, Ghanshyam Das Gupta, Preeti Patel

{"title":"Recent overview of microextraction of metal ions and pharmaceuticals by solidified floating organic drop microextraction techniques","authors":"Shivam Kumar, Sourabh Satapathy, B. Kurmi, Ghanshyam Das Gupta, Preeti Patel","doi":"10.1002/sscp.202400018","DOIUrl":"https://doi.org/10.1002/sscp.202400018","url":null,"abstract":"This work presents a comprehensive overview of solidified floating organic drop microextraction, with a particular emphasis on its applications in the analysis of heavy metal ions and different pharmaceutical compounds by sample pretreatment, complexation, and their identification by various analytical techniques. Here, we discussed basic principles, procedures, applications of the technique, and effects of different variables like pH, temperature, stirring rate, extraction time, salts addition, extraction solvent and extraction volume, centrifugation speed, and, vortex time on preconcentration. In brief, the review summarises the various modes of solidified floating organic drop microextraction such as dispersive liquid‐liquid microextraction, ultrasound‐assisted, air‐assisted liquid‐liquid microextraction, and vortex‐assisted solidified floating organic drop microextraction. this review covers the methods combined with analytical techniques such as inductively coupled plasma‐optical emission spectrometry, electrothermal atomic absorption spectrometry, flame atomic absorption spectroscopy, graphite furnace atomic absorption spectrometry, electrothermal vaporization inductively coupled plasma mass spectrometry, high‐performance liquid chromatography, and gas chromatography/mass spectrometry. among these, the incorporation of ultrasonic, vortex, and air agitation improves the dispersion mechanism. The coupling of solidified floating organic drop microextraction with other techniques enhances the selectivity and efficiency of the preconcentration procedure. This review provides a potential overview of the fundamental principles, advancements in various modes, influencing factors, and applications of solidified floating organic drop microextraction, showing its potential for advancements in sample preparation and analytical methodologies across various domains.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140081165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and characterization of vinyl silica‐supported surface molecularly imprinted polymers coupled with liquid chromatography‐mass spectrometry for extraction of prostaglandin analogs from cosmetic products 制备和表征乙烯基二氧化硅支撑表面分子印迹聚合物，并将其与液相色谱-质谱联用，用于提取化妆品中的前列腺素类似物

SEPARATION SCIENCE PLUS Pub Date : 2024-02-15 DOI: 10.1002/sscp.202300184

Aqeel Shahzad, Farzana Batool, Kamran Bashir, Jiameng Xu, Qiang Fu

{"title":"Preparation and characterization of vinyl silica‐supported surface molecularly imprinted polymers coupled with liquid chromatography‐mass spectrometry for extraction of prostaglandin analogs from cosmetic products","authors":"Aqeel Shahzad, Farzana Batool, Kamran Bashir, Jiameng Xu, Qiang Fu","doi":"10.1002/sscp.202300184","DOIUrl":"https://doi.org/10.1002/sscp.202300184","url":null,"abstract":"Prostaglandin analogs (PGAs) are commonly used to treat ocular hypertension and glaucoma patients. Nowadays, the use of PGAs enhances eyelashes in cosmetics, particularly in the condition of hypotrichosis. A method was developed in which surface molecularly imprinted polymers were synthesized for selective extraction of PGAs (bimatoprost, latanoprost, and travoprost) by using bimatoprost as template molecules which were coupled with liquid chromatography‐mass spectrometry (LC‐MS) system. Scanning electron microscopy and Fourier‐transform infrared spectroscopy techniques were used to study the morphological and physical characterization. The adsorption selectivity studies showed the high selectivity of PGAs. The adsorption capacities of bimatoprost, latanoprost, and travoprost were 93.8, 92.3, and 91.5 μg/g, respectively. The recoveries and precision of samples at three concentration levels (10–30 ng/mL) of bimatoprost, latanoprost, and travoprost were tested (98.5%–102.5%, 1.2%–4.5%, n = 3). The % relative standard deviation for repeatability of the method for bimatoprost, latanoprost, and travoprost ranged from 1.8 to 5.6, 1.5 to 4.8, and 1.6 to 5.2, respectively. While the limit of detection for bimatoprost, latanoprost, and travoprost was 0.20, 0.25, and 0.45 ng/mL, respectively. The method of surface molecularly imprinted polymer coupled with LC‐MS was successfully applied to cosmetic products for selective extraction of PGAs.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139963794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reverse‐phase high‐performance liquid chromatography methodology for film‐forming mafenide spray: Compatibility, assay, and in‐vitro release evaluation using Franz vertical diffusion cell apparatus 成膜马芬尼喷雾剂的反相高效液相色谱法：使用弗朗兹垂直扩散池装置进行兼容性、检测和体外释放评估

SEPARATION SCIENCE PLUS Pub Date : 2024-02-02 DOI: 10.1002/sscp.202300148

R. Mahajan, Sai Teja Chandragiri, Vivek Borse, Gananadhamu Samanthula, Amit Asthana

{"title":"Reverse‐phase high‐performance liquid chromatography methodology for film‐forming mafenide spray: Compatibility, assay, and in‐vitro release evaluation using Franz vertical diffusion cell apparatus","authors":"R. Mahajan, Sai Teja Chandragiri, Vivek Borse, Gananadhamu Samanthula, Amit Asthana","doi":"10.1002/sscp.202300148","DOIUrl":"https://doi.org/10.1002/sscp.202300148","url":null,"abstract":"This paper described a short and precise reverse‐phase high‐performance liquid chromatography (HPLC) method for quantifying mafenide in a film‐forming spray and preliminary in‐vitro release study. Following the International Council for Harmonization guidelines Q2 (R1), the method was validated. The chromatographic separation was achieved using a 10 mM potassium dihydrogen phosphate solution (mobile phase A) mixed with HPLC‐grade methanol (mobile phase B) in an 85:15 v/v ratio. Inert Sustain C8 (4.6 × 250 mm), 5 μm column was selected as the stationary phase. The flow rate was set as 0.8 mL/min, and detection was carried out at a wavelength of 222 nm. The developed HPLC method showed an accuracy between 98% and 102%. The primary diffusion study of film‐forming spray was performed using the Franz vertical diffusion cell apparatus. It was observed that an average of 26.94% of drug releases at 24 h. This indicates the drug has a slower release rate and shows local pharmacological action. The Weibull dissolution model was more fitting with regression square 0.9953. Furthermore, the drug excipient compatibility study revealed no interactions as the only drug peak was found in the chromatographic method, indicating that the chosen excipients were appropriate for a stable formulation.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139683520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissection of the potential immunomodulatory activity materials and mechanism of Wu Shen Wan (a classical traditional Chinese medicine prescription) based on in vivo substances profiling and network pharmacology 基于体内物质谱和网络药理学的乌参丸（一种经典中药处方）潜在免疫调节活性物质和机制的研究

SEPARATION SCIENCE PLUS Pub Date : 2024-01-15 DOI: 10.1002/sscp.202300155

Haiqiang Zhang, Jinqin Han, Qiao Li, Jianing Nie, Luanwei Han, Yang Yu, Chongning Lv, Jincai Lu

{"title":"Dissection of the potential immunomodulatory activity materials and mechanism of Wu Shen Wan (a classical traditional Chinese medicine prescription) based on in vivo substances profiling and network pharmacology","authors":"Haiqiang Zhang, Jinqin Han, Qiao Li, Jianing Nie, Luanwei Han, Yang Yu, Chongning Lv, Jincai Lu","doi":"10.1002/sscp.202300155","DOIUrl":"https://doi.org/10.1002/sscp.202300155","url":null,"abstract":"In the present study, a comprehensive strategy based on substances profiling in vivo and network pharmacology method was applied to screen out the potential immunomodulatory activity substances and mechanism of Wu Shen Wan. As a result, a total of 121 constituents were identified or tentatively characterized, including 26 flavonoids, 16 alkaloids, 10 tanshinones, four diterpenes, seven iridoid glycosides, eight coumarin, 18 phenolic acids, 29 ginsenosides, and three others. Moreover, 57 prototypes were clearly screened out in rat plasma and urine after ingestion of Wu Shen Wan. The Wu Shen Wan exhibited immunomodulatory effects via significantly increasing the phagocytic activity of macrophages and inducing the release of nitric oxide, tumor necrosis factor‐α, and interleukin 6 in RAW264.7 cells. In addition, the network pharmacology results showed that the top 20 Kyoto Encyclopedia of Genes and Genomes clusters were enriched, such as mitogen‐activated protein kinases pathway. This is the first systematic study on the chemical constituents of Wu Shen Wan. The analytical method features a quick and comprehensive dissection of the chemical composition and provides a basis for exploring its immunomodulatory effects.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139622556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioanalytical method optimization for simultaneous quantification of structurally related probe drugs in a phenotyping cocktail using liquid chromatography‐tandem mass spectrometry 利用液相色谱-串联质谱法同时定量表型鸡尾酒中结构相关探针药物的生物分析方法优化

SEPARATION SCIENCE PLUS Pub Date : 2024-01-11 DOI: 10.1002/sscp.202300241

Machel Leuschner, Duncan Cromarty

{"title":"Bioanalytical method optimization for simultaneous quantification of structurally related probe drugs in a phenotyping cocktail using liquid chromatography‐tandem mass spectrometry","authors":"Machel Leuschner, Duncan Cromarty","doi":"10.1002/sscp.202300241","DOIUrl":"https://doi.org/10.1002/sscp.202300241","url":null,"abstract":"The physicochemical diversity of the structurally related aromatic probe drugs, used together in a drug cocktail to assess metabolic and transport phenotypes, require optimized analytical procedures for simultaneous quantification. The analytical conditions can greatly influence the analyte selectivity, retention, stability, and ultimately the robustness of the method. The aim of this study was to assess the selectivity of the structurally related ionizable analytes between the commonly used C18 column chemistry and an alternative biphenyl column chemistry as well as the influence of changes in the analytical conditions on method robustness using liquid chromatography‐tandem mass spectrometry. A repeated measure two‐factor analysis of variance with Geisser‐Greenhouse correction was used to determine statistical significance. The results showed that a biphenyl stationary phase in combination with methanol as the organic eluent, could provide improved resolution and analyte selectivity. Changes in analytical conditions caused statistically significant variation in the retention behavior, selectivity, column efficiency, and sensitivity of the analytes of interest The robustness experiment confirmed the importance of controlling analytical conditions to ensure the reproducibility and reliability of the quantitative method.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical quality by design aided stability indicating a robust ultra‐performance liquid chromatographic technique for the quantification of sonidegib and its organic impurities in bulk drug substance 以设计辅助分析质量稳定性，显示用于定量散装药物中索尼地布及其有机杂质的稳健超高效液相色谱技术

SEPARATION SCIENCE PLUS Pub Date : 2024-01-11 DOI: 10.1002/sscp.202300181

Kasturi Rajashekhar, Challa Gangu Naidu, Chebolu Naga Sesha Sai Pavan Kumar, Bondigalla Ramachandra, Raju Padiya

{"title":"Analytical quality by design aided stability indicating a robust ultra‐performance liquid chromatographic technique for the quantification of sonidegib and its organic impurities in bulk drug substance","authors":"Kasturi Rajashekhar, Challa Gangu Naidu, Chebolu Naga Sesha Sai Pavan Kumar, Bondigalla Ramachandra, Raju Padiya","doi":"10.1002/sscp.202300181","DOIUrl":"https://doi.org/10.1002/sscp.202300181","url":null,"abstract":"A simple quality by design aided stability indicating method was developed for quantification of sonidegib (SONI) and its process related impurities using on ultra‐performance liquid chromatography in bulk drug substance. AutoChrom and Design‐Expert software were used to predict physicochemical properties, draw Ionization graphs, and generate analytical target profile. SONI was subjected to forced degradation conditions, such as oxidative, acid hydrolysis, base hydrolysis, hydrolytic, thermal, and photolytic hydrolysis. All degradation products and process contaminants were separated using an Acquity Ethylene Bridged Hybrid C18 column in gradient elution mode with a mobile phase containing 0.02 M ammonium acetate buffer and acetonitrile: methanol (80:20 v/v). The predicted physicochemical properties are accurate and they facilitated for selection of robust conditions in development of chromatographic method with minimal trials. The developed method can be used for quantification of drug and its process related impurities in bulk drugs.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139625869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress degradation study of Etoricoxib, isolation, and characterization of major degradation impurity by preparative high‐performance liquid chromatography, liquid chromatography‐mass spectrometry, and nuclear magnetic resonance: Validation of ultra‐performance liquid chromatography method 采用制备型高效液相色谱法、液相色谱-质谱法和核磁共振法研究依托考昔的应力降解、主要降解杂质的分离和特征：超高效液相色谱法的验证

SEPARATION SCIENCE PLUS Pub Date : 2024-01-09 DOI: 10.1002/sscp.202300106

Babu Reddy Goguladinne, Pulipaka Shyamala, K. M. V. N. Rao

{"title":"Stress degradation study of Etoricoxib, isolation, and characterization of major degradation impurity by preparative high‐performance liquid chromatography, liquid chromatography‐mass spectrometry, and nuclear magnetic resonance: Validation of ultra‐performance liquid chromatography method","authors":"Babu Reddy Goguladinne, Pulipaka Shyamala, K. M. V. N. Rao","doi":"10.1002/sscp.202300106","DOIUrl":"https://doi.org/10.1002/sscp.202300106","url":null,"abstract":"Etoricoxib (ETO) is a selective cyclooxygenase‐2 inhibitor, used as a nonsteroidal anti‐inflammatory drug. In this study, a detailed investigation was conducted on the degradation behavior of ETO under acidic, basic, neutral, photolytic, oxidative, and thermal degradation conditions as per the International Conference on Harmonization (ICH) guideline Q1A (R2). A gradient “ultra‐performance liquid chromatography (UPLC)” method was developed for separating the degradation products formed under various degradation conditions along with process‐related impurities. Acquity BEH phenyl column was used for the separation of analytes and 0.1% formic acid in water and 0.05% formic acid in acetonitrile:methanol (8:2 %v/v) were used as mobile phase‐A and mobile phase‐B. A major degradant namely, N‐oxide impurity was observed in oxidative degradation along with two minor degradants where N‐oxide was confirmed based on LC‐mass spectrometry (LC‐MS) studies. Further, the major degradant was enriched and isolated using preparative HPLC and the conformation of the N‐oxide impurity was established using nuclear magnetic resonance techniques including two‐dimensional studies and high‐resolution MS. In addition, the method for related substances by UPLC has been validated by considering a major degradant, namely N‐oxide along with process‐related impurities as per the ICH guidelines validation parameters.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139444689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a simple and fast method named solvent‐assisted dispersive solid phase extraction for trace detection of triazole fungicides in water, fruit, vegetable, and agricultural soil samples 开发一种简单快速的溶剂辅助分散固相萃取法，用于痕量检测水、水果、蔬菜和农业土壤样品中的三唑类杀菌剂

SEPARATION SCIENCE PLUS Pub Date : 2024-01-07 DOI: 10.1002/sscp.202300191

Zolfaghar Aladaghlo, Ali Sahragard, Alireza Fakhari

{"title":"Development of a simple and fast method named solvent‐assisted dispersive solid phase extraction for trace detection of triazole fungicides in water, fruit, vegetable, and agricultural soil samples","authors":"Zolfaghar Aladaghlo, Ali Sahragard, Alireza Fakhari","doi":"10.1002/sscp.202300191","DOIUrl":"https://doi.org/10.1002/sscp.202300191","url":null,"abstract":"Solvent‐assisted dispersive solid‐phase extraction (SA‐DSPE) approach was developed to measure triazole fungicides (TFs). In the SA‐DSPE technique, the addition of 1000 μL of ethanol as a disperser solvent, along with a small quantity of benzil as a sorbent to the sample solution, led to a cloudy solution. After completion of the extraction, the mixture was subjected to centrifugation to isolate benzil. Next, benzil was dissolved in ethanol, and the resulting solution was subsequently analyzed by a gas chromatography‐flame ionization detector. This method demonstrated high linearity (R2 > 0.9963) and repeatability (relative standard deviation % < 4.3) for the quantification of TFs under the optimal conditions (sorbent: benzil, amount of benzil: 2% w/v, pH of solution: 7.0, disperser solvent: ethanol, volumes of ethanol: 1000 μL, centrifuge time: 3 min, extraction temperature: 25°C, and ionic strength: without salt addition). The proposed SA‐DSPE yielded detection limits, quantification limits, and preconcentration factors within the ranges of 0.3–0.9 ng/mL, 1.0–3.0 ng/mL, and 419–426, respectively. Finally, the validated method was employed to determine TFs in a diverse range of real samples, encompassing waters, fruits, vegetables, and agricultural soils, with relative recoveries ranging from 93.0% to 104%.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139448387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validated high‐performance thin‐layer chromatographic densitometric method for quantification of gallic acid, quercetin, and ursolic acid in the combined formulation of Emblica officinalis, Punica granatum Linn, and Ocimum sanctum Linn extracts 经验证的高效薄层色谱密度测量法，用于定量分析恩布利卡、肉桂和洋甘菊提取物复方制剂中的没食子酸、槲皮素和熊果酸含量

SEPARATION SCIENCE PLUS Pub Date : 2024-01-04 DOI: 10.1002/sscp.202300083

K. Patel, Gandhi Vasima, P. Shah, V. Thakkar

{"title":"Validated high‐performance thin‐layer chromatographic densitometric method for quantification of gallic acid, quercetin, and ursolic acid in the combined formulation of Emblica officinalis, Punica granatum Linn, and Ocimum sanctum Linn extracts","authors":"K. Patel, Gandhi Vasima, P. Shah, V. Thakkar","doi":"10.1002/sscp.202300083","DOIUrl":"https://doi.org/10.1002/sscp.202300083","url":null,"abstract":"A quantitative high‐performance thin‐layer chromatography method has been developed for the estimation of three markers gallic acid, quercetin, and ursolic acid in the extracts and combined formulation of Emblica officinalis, Punica granatum Linn, and Ocimum sanctum Linn extracts. Chromatographic development was performed using precoated silica gel 60 F254 TLC plate and final optimized mobile phase as toluene:ethyl acetate:formic acid (4.5:3:0.2 v/v/v), resulting in optimum resolution with Rf of 0.29, 0.51, and 0.78 for gallic acid, quercetin, and ursolic acid, respectively. Gallic acid and quercetin were detected at 258 nm and ursolic acid at 529 nm after derivatization. All markers showed a good correlation coefficient of 0.9950 for gallic acid, 0.9953 for quercetin, and 0.9945 for ursolic acid. The accuracy and precision measured were less than 2% relative standard deviation for all markers. The mean percent recovery found for gallic acid, quercetin, and ursolic acid were within acceptable limits in formulation indicating that the excipients present have no interference. The proposed method was found to be specific, accurate, linear, precise, and robust, and can be applicable for the simultaneous estimation of gallic acid, quercetin, and ursolic acid for the analysis of individual plant extracts and herbal formulation.","PeriodicalId":508518,"journal":{"name":"SEPARATION SCIENCE PLUS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139386237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0