Anatomical Record最新文献

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Architecture of the human jaw-closing and jaw-opening muscles. 人类合下巴和开下巴肌肉的结构。
4区 医学
Anatomical Record Pub Date : 1997-07-01 DOI: 10.1002/(sici)1097-0185(199707)248:3<464::aid-ar20>3.3.co;2-4
T M Van Eijden, J A Korfage, P Brugman
{"title":"Architecture of the human jaw-closing and jaw-opening muscles.","authors":"T M Van Eijden,&nbsp;J A Korfage,&nbsp;P Brugman","doi":"10.1002/(sici)1097-0185(199707)248:3<464::aid-ar20>3.3.co;2-4","DOIUrl":"https://doi.org/10.1002/(sici)1097-0185(199707)248:3<464::aid-ar20>3.3.co;2-4","url":null,"abstract":"<p><strong>Background: </strong>The human jaw-closing and jaw-opening muscles produce forces leading to the development of three-dimensional bite and chewing forces and to three-dimensional movements of the jaw. The length of the sarcomeres is a major determinant for both force and velocity, and the maximal work, force, and shortening range each muscle is capable of producing are proportional to the architectural parameter volume, physiological cross-sectional area, and fiber length, respectively. In addition, the mechanical role the muscles play is strongly related to their three-dimensional position and orientation in the muscle-bone-joint system. The objective of this study was to compare relevant architectural characteristics for the jaw-closing and jaw-opening muscles and to provide a set of data that can be used in biomechanical modeling of the masticatory system.</p><p><strong>Methods: </strong>In eight cadavers, sarcomere lengths, muscle masses, fiber lengths, pennation angles, and physiological cross-sectional areas were determined for the following muscles: superficial and deep masseter, anterior and posterior temporalis, anterior and posterior medial pterygoid, inferior and superior lateral pterygoid, posterior and anterior digastric, geniohyoid, posterior and anterior mylohyoid, and stylohyoid. To determine the spatial position of their action lines, the three-dimensional coordinates of the attachment sites were registered.</p><p><strong>Results: </strong>Compared with the jaw openers, the jaw closers were characterized by shorter sarcomere lengths at the closed jaw, larger masses of contractile and tendinous tissue, larger physiological cross-sectional areas, larger pennation angles, shorter fiber lengths, shorter moment arms, and lower fiber-length-to-muscle-length ratios. In addition, architectural features differed across the muscles of the same functional group. Sarcomere length did not differ significantly among the regions of the same muscle. In contrast, in some muscles, significant intramuscular differences were found with respect to, e.g., physiological cross-sectional area, fiber length, pennation angle, and moment arm length.</p><p><strong>Conclusions: </strong>The results suggest that the jaw-closing muscles have architectural features that suit them for force production. Conversely, the jaw-opening muscles are better designed to produce velocity and displacement.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"248 3","pages":"464-74"},"PeriodicalIF":0.0,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20159308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Architecture, elastic fiber, and collagen in the distal air portion of the lung of the 18-month-old rat. 18月龄大鼠肺远端空气部分的结构、弹性纤维和胶原蛋白。
4区 医学
Anatomical Record Pub Date : 1997-05-01 DOI: 10.1002/(sici)1097-0185(199705)248:1<63::aid-ar7>3.3.co;2-s
J D Escolar, C Tejero, M A Escolar, F Montalvo, R Garisa
{"title":"Architecture, elastic fiber, and collagen in the distal air portion of the lung of the 18-month-old rat.","authors":"J D Escolar,&nbsp;C Tejero,&nbsp;M A Escolar,&nbsp;F Montalvo,&nbsp;R Garisa","doi":"10.1002/(sici)1097-0185(199705)248:1<63::aid-ar7>3.3.co;2-s","DOIUrl":"https://doi.org/10.1002/(sici)1097-0185(199705)248:1<63::aid-ar7>3.3.co;2-s","url":null,"abstract":"<p><strong>Background: </strong>The increase in the distal air spaces which takes place with age is the only sufficiently documented datum for differentiation between the senile and the adult lung. There are other pulmonary components which may be modified as the lung enters the phase of old age, but they have not as yet been sufficiently studied. The purpose of this study is to make a morphometric comparison between the wall thickness of the distal air spaces, elastic fiber, and collagen in lungs of rats of between 5 and 18 months of age.</p><p><strong>Methods: </strong>The left lung of each rat was histologically processed for light microscopy. The sections were contrasted using methylene blue, resorcinfuchsin and Sirius red. Systematic randomized sampling was used for the selection of the histological fields studied. Morphometric variables were studied, and were systematized into three groups, namely: variables related with the alveolar architecture, variables which quantify elastic fiber, and those which quantify collagen.</p><p><strong>Results: </strong>The old animals exhibited significant differences (p < 0.05) in the following variables: 1. In relation with the alveolar architecture, mean linear intercept, alveolar chord and wall thickness increased, whereas internal alveolar perimeter and tissue density decreased. 2. The relation elastic fiber density/lung tissue density increased. None of the variables which quantify collagen displayed significant differences.</p><p><strong>Conclusion: </strong>Our data lead us to consider that the lungs of the old animals displayed, not only an enlargement of the distal air spaces, but also a thickening of the alveolar wall and an increase in elastic fiber in relation to the rest of the lung tissue.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"248 1","pages":"63-9"},"PeriodicalIF":0.0,"publicationDate":"1997-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20091391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Distribution of bursal secretory dendritic cells in the chicken. 鸡法氏囊分泌性树突状细胞的分布。
4区 医学
Anatomical Record Pub Date : 1996-11-01 DOI: 10.1002/(SICI)1097-0185(199611)246:3<372::AID-AR8>3.0.CO;2-#
M Gallego, E Del Cacho, C Felices, A Varas, J A Bascuas
{"title":"Distribution of bursal secretory dendritic cells in the chicken.","authors":"M Gallego,&nbsp;E Del Cacho,&nbsp;C Felices,&nbsp;A Varas,&nbsp;J A Bascuas","doi":"10.1002/(SICI)1097-0185(199611)246:3<372::AID-AR8>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199611)246:3<372::AID-AR8>3.0.CO;2-#","url":null,"abstract":"<p><strong>Background: </strong>The bursa of Fabricius provided the microenvironment for B-cell differentiation. Continuous contact between lymphoid cells and antigen in the bursa further suggested that antigenic material has an important influence on the maintenance and development of B cells in the bursa. In addition, a dendritic cell, the bursal secretory dendritic cell (BSDC), has been identified in the medulla. The hypothesis that, in the bursal follicles, the contact between the lymphoid cells and the antigen may be mediated by dendritic cells prompted us to identify a bursal dendritic cell that becomes activated after contact with the antigen.</p><p><strong>Methods: </strong>A polyclonal antiserum to S-100 protein was used to identify bursal dendritic cells because S-100 protein, a calcium-binding protein, has been shown to be a marker for the identification of chicken dendritic cells following recent contact with antigen.</p><p><strong>Results: </strong>At every age investigated, S-100-positive cells showed a location and shape identical to those described for BSDCs. Positive cells were found within and under the follicle-associated epithelial cells (FAE), indicating that these cells were strategically placed where they would encounter the antigen. In addition, positive cells were found arranged along the corticomedullary junction, which is a regenerative zone for the BSDC. After 10 weeks of age, the number of positive cells dramatically decreased, suggesting that the endocytic activity of the FAE may become impaired as the bursa regresses.</p><p><strong>Conclusions: </strong>The polyclonal antiserum to S-100 protein identified the BSDCs in the bursal follicles. Positive cells may be BSDCs that have undergone a functional activation after contact with the antigen. These cells may have a role as antigen-presenting cells in the bursal follicles. Hence, these cells may be involved in the events that lead to B-cell differentiation.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"246 3","pages":"372-6"},"PeriodicalIF":0.0,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199611)246:3<372::AID-AR8>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19879378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Ultrastructural characterization of preosteoclasts derived from bone marrow progenitors stimulated by osteoclast colony stimulating factor. 破骨细胞集落刺激因子刺激骨髓祖细胞的超微结构特征。
4区 医学
Anatomical Record Pub Date : 1996-10-01 DOI: 10.1002/(SICI)1097-0185(199610)246:2<176::AID-AR4>3.0.CO;2-#
M Y Lee, M Jonas, J L Lottsfeldt, E Y Chi
{"title":"Ultrastructural characterization of preosteoclasts derived from bone marrow progenitors stimulated by osteoclast colony stimulating factor.","authors":"M Y Lee,&nbsp;M Jonas,&nbsp;J L Lottsfeldt,&nbsp;E Y Chi","doi":"10.1002/(SICI)1097-0185(199610)246:2<176::AID-AR4>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199610)246:2<176::AID-AR4>3.0.CO;2-#","url":null,"abstract":"<p><strong>Background: </strong>Osteoclast colony stimulating factor (O-CSF) is an osteoclast-specific growth factor that stimulates the clonal growth of primitive osteoclast progenitors from bone marrow cells in culture. To characterize the morphology of immature osteoclasts (preosteoclasts) arising from complex hematopoietic tissues, progenies of O-CSF-responsive progenitors were cocultured with devitalized calvariae, and their cytochemical and ultrastructural features were examined.</p><p><strong>Methods: </strong>Murine bone marrow cells were cultured in semisolid medium for 14 days in the presence of O-CSF. Colonies derived from osteoclast progenitors were then cocultured with devitalized mouse calvariae for 5 days. Cells attached to the calvariae were stained for tartrate resistant acid phosphatase (TRAPase), and the ultrastructure of these cells was examined by transmission electron microscopy. Bone marrow cells stimulated by macrophage colony stimulating factor (M-CSF) were similarly studied as a control.</p><p><strong>Results: </strong>O-CSF-induced preosteoclasts stained strongly positive for TRAPase when cocultured with calvariae. These cells showed single nuclei, and their cytoplasm contained numerous mitochondria, vacuoles, granules, and coated vesicles. The ruffled cell border consisted of short, blunt, fingerlike projections. The adjacent clear zone contained abundant microtubules, microfilaments, and long narrow channels. M-CSF-induced macrophages were TRAPase negative, with no ruffled borders or clear zones.</p><p><strong>Conclusions: </strong>All the characteristic features of active osteoclasts were observed in the cells derived from O-CSF-responsive bone marrow progenitors except that these cells were mononucleated, and their ruffled borders were not fully convoluted, indicative of their immature nature. This study documents for the first time the ultrastructural characteristics of preosteoclasts derived from cultured bone marrow progenitors in early stages of development.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"246 2","pages":"176-84"},"PeriodicalIF":0.0,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199610)246:2<176::AID-AR4>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19853705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Modulation of autotransplanted adrenal gland by endothelin-1: a morphological and biochemical study. 内皮素-1调节自体移植肾上腺:形态学和生化研究。
4区 医学
Anatomical Record Pub Date : 1996-09-01 DOI: 10.1002/(SICI)1097-0185(199609)246:1<98::AID-AR11>3.0.CO;2-#
P Vendeira, D Neves, M M Magalhães, M C Magalhães
{"title":"Modulation of autotransplanted adrenal gland by endothelin-1: a morphological and biochemical study.","authors":"P Vendeira,&nbsp;D Neves,&nbsp;M M Magalhães,&nbsp;M C Magalhães","doi":"10.1002/(SICI)1097-0185(199609)246:1<98::AID-AR11>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199609)246:1<98::AID-AR11>3.0.CO;2-#","url":null,"abstract":"<p><strong>Background: </strong>Adrenal gland autotransplantation, a model of cortical tissue regeneration, provides the reconstruction of distinct functional and morphological zonae. A morphological and biochemical study of the adrenal gland of adult male rats after autotransplantation and endothelin-1 (ET-1) administration was made.</p><p><strong>Methods: </strong>The technique involved bilateral adrenalectomy and placement of pieces of the adrenal gland in a dorsal plane between the skin and muscle. The animals were killed 90 days after the autotransplantation and 1 hr after intravenous ET-1 administration (0.5 microgram/kg body weight). The autotransplanted pieces were removed, fixed, and processed for light and electron microscopic morphologic studies. Trunk blood was collected for steroid assay.</p><p><strong>Results: </strong>Saline-treated control autotransplanted animals showed no remarkable differences in adrenal organization; grafts exhibiting a mass of regenerated cortical tissue were arranged in nests of glandular cells surrounded by a fibrous capsule and intersected by layers of connective tissue. The adrenal medulla was systematically absent. Ultrastructure of ET-1-treated animals revealed an inner area in the graft, consisting mainly of fasciculatalike cells. Cytoplasmic changes were evident, with high variations in mitochondrial size and arrangement. Profiles of smooth endoplasmic reticulum sometimes exhibited evidence of hypertrophy. Glandular cells in the graft outer area (subcapsular) were almost invariably like glomerulosa; however, some of them showed mitochondria with a peculiar arrangement of the cristae. \"Hybrid\" cells with mitochondria resembling those of the zona reticularis were also observed in the subcapsular environment. ET-1-stimulated animals showed significant increases in plasma corticosterone and aldosterone concentrations.</p><p><strong>Conclusions: </strong>Endothelin-1, previously reported to stimulate acutely the aldosterone secretion by the adrenal zona glomerulosa in the rat, seems to exert a modulator role on the physiology of adrenal autotransplants, their regeneration and secretion.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"246 1","pages":"98-106"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199609)246:1<98::AID-AR11>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19842365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Evolution in tooth developmental biology: of morphology and molecules. 牙齿发育生物学的进化:形态学和分子。
4区 医学
Anatomical Record Pub Date : 1996-06-01 DOI: 10.1002/(SICI)1097-0185(199606)245:2<131::AID-AR3>3.0.CO;2-#
H C Slavkin, T Diekwisch
{"title":"Evolution in tooth developmental biology: of morphology and molecules.","authors":"H C Slavkin,&nbsp;T Diekwisch","doi":"10.1002/(SICI)1097-0185(199606)245:2<131::AID-AR3>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199606)245:2<131::AID-AR3>3.0.CO;2-#","url":null,"abstract":"","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"245 2","pages":"131-50"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199606)245:2<131::AID-AR3>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19742516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Molecular mechanisms of cell and tissue interactions during early tooth development. 早期牙齿发育过程中细胞和组织相互作用的分子机制。
4区 医学
Anatomical Record Pub Date : 1996-06-01 DOI: 10.1002/(SICI)1097-0185(199606)245:2<151::AID-AR4>3.0.CO;2-#
I Thesleff, A Vaahtokari, S Vainio, A Jowett
{"title":"Molecular mechanisms of cell and tissue interactions during early tooth development.","authors":"I Thesleff,&nbsp;A Vaahtokari,&nbsp;S Vainio,&nbsp;A Jowett","doi":"10.1002/(SICI)1097-0185(199606)245:2<151::AID-AR4>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199606)245:2<151::AID-AR4>3.0.CO;2-#","url":null,"abstract":"<p><strong>Background: </strong>Morphogenesis and cell differentiation during the development of all organs, including the tooth, are regulated by interactions between cells and tissues. The developing tooth is one of the organs in which the molecular mechanisms of such interactions are starting to be elucidated.</p><p><strong>Results: </strong>Homotypic cell interactions take place between cells of the same developmental history, and they are a central mechanism in the formation of mesenchymal cell condensates during the bud stage of tooth development. Syndecan-1, a cell surface heparan sulfate proteoglycan, is transiently expressed in the dental mesenchyme and may regulate dental mesenchymal cell condensation. It binds tenascin, a matrix glycoprotein abundant in dental mesenchyme, suggesting involvement of cell-matrix interactions. Syndecan also binds growth factors, and its association with cell proliferation in the dental mesenchyme suggests roles in the regulation of cell number in the condensing cells. Inductive interactions between the epithelial and mesenchymal tissues regulate tooth development at all stages. In the early dental mesenchyme, the expression of several molecules, including syndecan and tenascin, are regulated by the epithelium. There is evidence that growth factors act as diffusible signals mediating these interactions. BMP-2 and BMP-4 (bone morphogenetic proteins), which belong to the TGF beta superfamily, are expressed in the early dental epithelium, and their effects on the dental mesenchyme mimic those of the epithelium. In particular, BMPs induce the expression of the homeobox-containing transcription factors Msx-1 and Msx-2 in the dental mesenchyme.</p><p><strong>Conclusions: </strong>Based on current knowledge about the molecular changes accompanying tooth development and the results of experimental studies, we present a model for molecular regulation of early tooth development.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"245 2","pages":"151-61"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199606)245:2<151::AID-AR4>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19742517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 76
Cellular distribution patterns of lanthanum and morphometry of rat hearts exposed to different degrees of ischemic stress. 不同程度缺血应激大鼠心脏镧的细胞分布规律及形态测定。
4区 医学
Anatomical Record Pub Date : 1995-12-01 DOI: 10.1002/ar.1092430412
A Schmiedl, F Bach, H Fehrenbach, P A Schnabel, J Richter
{"title":"Cellular distribution patterns of lanthanum and morphometry of rat hearts exposed to different degrees of ischemic stress.","authors":"A Schmiedl,&nbsp;F Bach,&nbsp;H Fehrenbach,&nbsp;P A Schnabel,&nbsp;J Richter","doi":"10.1002/ar.1092430412","DOIUrl":"https://doi.org/10.1002/ar.1092430412","url":null,"abstract":"<p><strong>Background: </strong>The element lanthanum (La) can be used as a tracer for verification of membrane permeability. The aim of this study was to establish whether 1) distribution of La in the myocardium of rat hearts depends on the degree of ischemic stress and 2) morphometrically determined cell and mitochondrial swelling correlates with the La distribution.</p><p><strong>Materials and methods: </strong>Isolated beating rat hearts were arrested by coronary perfusion with the cardioplegic solution Custodiol (controls) or by aortic cross clamping followed by exposure to different degrees of ischemic stress. The solutions for perfusion-and postfixation as well as for rinsing contained 1.1% La(NO3)3. Cellular and mitochondrial swelling were determined morphometrically and myocytes exhibiting intracellular La were quantified and stated as percentage of test fields.</p><p><strong>Results: </strong>Immediately after cardiac arrest La was present as precipitates only in a few myocytes adjacent to the outer mitochondrial membrane as seen by cTEM and ESI. In such cells La was also detected by EELS in mitochondrial matrix and myofibrils. Advanced ischemic stress led to an increase of the percentage of myocytes containing detectable intracellular La. After 45 min ischemia at 30 degrees C, myocytes and mitochondria showed a remarkable edema and different intracellular distribution patterns of La. After 90 min of ischemia at 20 degrees C interruptions of sarcolemma could only be detected in a few of the swollen myocytes. Roundish La granules were seen in the myofibrils. The percentage of myocytes containing intracellular La and the extent of cellular and mitochondrial swelling showed a significant correlation.</p><p><strong>Conclusions: </strong>Patterns of intracellular La distribution depend on the degree of ischemic stress and correspond to the degree of cellular as well as mitochondrial edema. These results point at a direct relation between alterations of membrane permeability and development of edema.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"496-508"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430412","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19577382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Sperm nuclei entering parthenogenetically activated mouse oocytes before the first mitosis transform into pronuclei. An ultrastructural study. 在第一次有丝分裂转化为原核之前,精子核进入孤雌活化的小鼠卵母细胞。超微结构研究。
4区 医学
Anatomical Record Pub Date : 1995-12-01 DOI: 10.1002/ar.1092430414
M Maleszewski
{"title":"Sperm nuclei entering parthenogenetically activated mouse oocytes before the first mitosis transform into pronuclei. An ultrastructural study.","authors":"M Maleszewski","doi":"10.1002/ar.1092430414","DOIUrl":"https://doi.org/10.1002/ar.1092430414","url":null,"abstract":"<p><strong>Background: </strong>This report is an extension of previous observations (Maleszewski 1992. Mol. Reprod. Dev., 33:215-221) on the behavior of mouse sperm nuclei incorporated into parthenogenetically activated mouse oocytes prior to the first cleavage division and undergoing transformation during mitosis.</p><p><strong>Method: </strong>Artificially activated mouse oocytes were inseminated in vitro and an ultrastructural analysis was performed of sperm-derived nuclei present in two parthenogenetic two-cell embryos.</p><p><strong>Results: </strong>Both chromatin and nuclear envelope of sperm derived-nuclei are structurally identical with those of oocyte-derived nuclei and of the nuclei of blastomeres of normal two-cell embryos.</p><p><strong>Conclusions: </strong>Cytoplasm of the parthenogenote during the first mitotic division has the ability to transform sperm nucleus into a male pronucleus just like the cytoplasm of a metaphase II oocyte.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"516-8"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430414","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19576474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Reducing the levels of formaldehyde exposure in gross anatomy laboratories. 降低大体解剖实验室的甲醛暴露水平。
4区 医学
Anatomical Record Pub Date : 1995-12-01 DOI: 10.1002/ar.1092430417
R Coleman
{"title":"Reducing the levels of formaldehyde exposure in gross anatomy laboratories.","authors":"R Coleman","doi":"10.1002/ar.1092430417","DOIUrl":"https://doi.org/10.1002/ar.1092430417","url":null,"abstract":"<p><strong>Background: </strong>A method is described in which formaldehyde levels are greatly reduced in our gross anatomy laboratory in order to comply with increasingly severe safety and health regulations.</p><p><strong>Methods: </strong>A novel type of dissection \"bed\" has been introduced which incorporates an internal motor that causes a downflow of formaldehyde-rich vapors, which are absorbed by a replaceable active carbon filtration system.</p><p><strong>Results: </strong>Use of the new dissection \"beds\" has resulted in the recirculated air being virtually formaldehyde-free. Formaldehyde vapor levels in our gross anatomy laboratory have been greatly reduced and are typically in the range of 0.03-0.09 ppm.</p><p><strong>Conclusions: </strong>The new system allows us to comply with safety and health regulations and provide a dissection room with an excellent working environment.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"531-3"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430417","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19576477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 35
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