Anatomical Record最新文献

{"title":"Modulation of autotransplanted adrenal gland by endothelin-1: a morphological and biochemical study.","authors":"P Vendeira,&nbsp;D Neves,&nbsp;M M Magalhães,&nbsp;M C Magalhães","doi":"10.1002/(SICI)1097-0185(199609)246:1<98::AID-AR11>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199609)246:1<98::AID-AR11>3.0.CO;2-#","url":null,"abstract":"<p><strong>Background: </strong>Adrenal gland autotransplantation, a model of cortical tissue regeneration, provides the reconstruction of distinct functional and morphological zonae. A morphological and biochemical study of the adrenal gland of adult male rats after autotransplantation and endothelin-1 (ET-1) administration was made.</p><p><strong>Methods: </strong>The technique involved bilateral adrenalectomy and placement of pieces of the adrenal gland in a dorsal plane between the skin and muscle. The animals were killed 90 days after the autotransplantation and 1 hr after intravenous ET-1 administration (0.5 microgram/kg body weight). The autotransplanted pieces were removed, fixed, and processed for light and electron microscopic morphologic studies. Trunk blood was collected for steroid assay.</p><p><strong>Results: </strong>Saline-treated control autotransplanted animals showed no remarkable differences in adrenal organization; grafts exhibiting a mass of regenerated cortical tissue were arranged in nests of glandular cells surrounded by a fibrous capsule and intersected by layers of connective tissue. The adrenal medulla was systematically absent. Ultrastructure of ET-1-treated animals revealed an inner area in the graft, consisting mainly of fasciculatalike cells. Cytoplasmic changes were evident, with high variations in mitochondrial size and arrangement. Profiles of smooth endoplasmic reticulum sometimes exhibited evidence of hypertrophy. Glandular cells in the graft outer area (subcapsular) were almost invariably like glomerulosa; however, some of them showed mitochondria with a peculiar arrangement of the cristae. \"Hybrid\" cells with mitochondria resembling those of the zona reticularis were also observed in the subcapsular environment. ET-1-stimulated animals showed significant increases in plasma corticosterone and aldosterone concentrations.</p><p><strong>Conclusions: </strong>Endothelin-1, previously reported to stimulate acutely the aldosterone secretion by the adrenal zona glomerulosa in the rat, seems to exert a modulator role on the physiology of adrenal autotransplants, their regeneration and secretion.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"246 1","pages":"98-106"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199609)246:1<98::AID-AR11>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19842365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution in tooth developmental biology: of morphology and molecules.","authors":"H C Slavkin,&nbsp;T Diekwisch","doi":"10.1002/(SICI)1097-0185(199606)245:2<131::AID-AR3>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199606)245:2<131::AID-AR3>3.0.CO;2-#","url":null,"abstract":"","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"245 2","pages":"131-50"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199606)245:2<131::AID-AR3>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19742516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of cell and tissue interactions during early tooth development.","authors":"I Thesleff,&nbsp;A Vaahtokari,&nbsp;S Vainio,&nbsp;A Jowett","doi":"10.1002/(SICI)1097-0185(199606)245:2<151::AID-AR4>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1097-0185(199606)245:2<151::AID-AR4>3.0.CO;2-#","url":null,"abstract":"<p><strong>Background: </strong>Morphogenesis and cell differentiation during the development of all organs, including the tooth, are regulated by interactions between cells and tissues. The developing tooth is one of the organs in which the molecular mechanisms of such interactions are starting to be elucidated.</p><p><strong>Results: </strong>Homotypic cell interactions take place between cells of the same developmental history, and they are a central mechanism in the formation of mesenchymal cell condensates during the bud stage of tooth development. Syndecan-1, a cell surface heparan sulfate proteoglycan, is transiently expressed in the dental mesenchyme and may regulate dental mesenchymal cell condensation. It binds tenascin, a matrix glycoprotein abundant in dental mesenchyme, suggesting involvement of cell-matrix interactions. Syndecan also binds growth factors, and its association with cell proliferation in the dental mesenchyme suggests roles in the regulation of cell number in the condensing cells. Inductive interactions between the epithelial and mesenchymal tissues regulate tooth development at all stages. In the early dental mesenchyme, the expression of several molecules, including syndecan and tenascin, are regulated by the epithelium. There is evidence that growth factors act as diffusible signals mediating these interactions. BMP-2 and BMP-4 (bone morphogenetic proteins), which belong to the TGF beta superfamily, are expressed in the early dental epithelium, and their effects on the dental mesenchyme mimic those of the epithelium. In particular, BMPs induce the expression of the homeobox-containing transcription factors Msx-1 and Msx-2 in the dental mesenchyme.</p><p><strong>Conclusions: </strong>Based on current knowledge about the molecular changes accompanying tooth development and the results of experimental studies, we present a model for molecular regulation of early tooth development.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"245 2","pages":"151-61"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1097-0185(199606)245:2<151::AID-AR4>3.0.CO;2-#","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19742517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular distribution patterns of lanthanum and morphometry of rat hearts exposed to different degrees of ischemic stress.","authors":"A Schmiedl,&nbsp;F Bach,&nbsp;H Fehrenbach,&nbsp;P A Schnabel,&nbsp;J Richter","doi":"10.1002/ar.1092430412","DOIUrl":"https://doi.org/10.1002/ar.1092430412","url":null,"abstract":"<p><strong>Background: </strong>The element lanthanum (La) can be used as a tracer for verification of membrane permeability. The aim of this study was to establish whether 1) distribution of La in the myocardium of rat hearts depends on the degree of ischemic stress and 2) morphometrically determined cell and mitochondrial swelling correlates with the La distribution.</p><p><strong>Materials and methods: </strong>Isolated beating rat hearts were arrested by coronary perfusion with the cardioplegic solution Custodiol (controls) or by aortic cross clamping followed by exposure to different degrees of ischemic stress. The solutions for perfusion-and postfixation as well as for rinsing contained 1.1% La(NO3)3. Cellular and mitochondrial swelling were determined morphometrically and myocytes exhibiting intracellular La were quantified and stated as percentage of test fields.</p><p><strong>Results: </strong>Immediately after cardiac arrest La was present as precipitates only in a few myocytes adjacent to the outer mitochondrial membrane as seen by cTEM and ESI. In such cells La was also detected by EELS in mitochondrial matrix and myofibrils. Advanced ischemic stress led to an increase of the percentage of myocytes containing detectable intracellular La. After 45 min ischemia at 30 degrees C, myocytes and mitochondria showed a remarkable edema and different intracellular distribution patterns of La. After 90 min of ischemia at 20 degrees C interruptions of sarcolemma could only be detected in a few of the swollen myocytes. Roundish La granules were seen in the myofibrils. The percentage of myocytes containing intracellular La and the extent of cellular and mitochondrial swelling showed a significant correlation.</p><p><strong>Conclusions: </strong>Patterns of intracellular La distribution depend on the degree of ischemic stress and correspond to the degree of cellular as well as mitochondrial edema. These results point at a direct relation between alterations of membrane permeability and development of edema.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"496-508"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430412","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19577382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm nuclei entering parthenogenetically activated mouse oocytes before the first mitosis transform into pronuclei. An ultrastructural study.","authors":"M Maleszewski","doi":"10.1002/ar.1092430414","DOIUrl":"https://doi.org/10.1002/ar.1092430414","url":null,"abstract":"<p><strong>Background: </strong>This report is an extension of previous observations (Maleszewski 1992. Mol. Reprod. Dev., 33:215-221) on the behavior of mouse sperm nuclei incorporated into parthenogenetically activated mouse oocytes prior to the first cleavage division and undergoing transformation during mitosis.</p><p><strong>Method: </strong>Artificially activated mouse oocytes were inseminated in vitro and an ultrastructural analysis was performed of sperm-derived nuclei present in two parthenogenetic two-cell embryos.</p><p><strong>Results: </strong>Both chromatin and nuclear envelope of sperm derived-nuclei are structurally identical with those of oocyte-derived nuclei and of the nuclei of blastomeres of normal two-cell embryos.</p><p><strong>Conclusions: </strong>Cytoplasm of the parthenogenote during the first mitotic division has the ability to transform sperm nucleus into a male pronucleus just like the cytoplasm of a metaphase II oocyte.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"516-8"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430414","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19576474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reducing the levels of formaldehyde exposure in gross anatomy laboratories.","authors":"R Coleman","doi":"10.1002/ar.1092430417","DOIUrl":"https://doi.org/10.1002/ar.1092430417","url":null,"abstract":"<p><strong>Background: </strong>A method is described in which formaldehyde levels are greatly reduced in our gross anatomy laboratory in order to comply with increasingly severe safety and health regulations.</p><p><strong>Methods: </strong>A novel type of dissection \"bed\" has been introduced which incorporates an internal motor that causes a downflow of formaldehyde-rich vapors, which are absorbed by a replaceable active carbon filtration system.</p><p><strong>Results: </strong>Use of the new dissection \"beds\" has resulted in the recirculated air being virtually formaldehyde-free. Formaldehyde vapor levels in our gross anatomy laboratory have been greatly reduced and are typically in the range of 0.03-0.09 ppm.</p><p><strong>Conclusions: </strong>The new system allows us to comply with safety and health regulations and provide a dissection room with an excellent working environment.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"531-3"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430417","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19576477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined vascular-bronchoalveolar casting using formalin-fixed canine lungs and a low viscosity silicone rubber.","authors":"J A Nettum","doi":"10.1002/ar.1092430410","DOIUrl":"https://doi.org/10.1002/ar.1092430410","url":null,"abstract":"<p><strong>Background: </strong>Previous work using unfixed or fixed tissues has shown that casts can be made that demonstrate the three-dimensional structure of tissues such as the bronchoalveolar tree or the vasculature. In this report, a new method for creating a vascular-bronchoalveolar cast is described.</p><p><strong>Method: </strong>Canine lungs were taken from storage in formalin. Silastic 734 RTV (room temperature vulcanizing) with added red or blue pigments was injected into the pulmonary arteries and veins, respectively, using compressed air. This was followed by filling the airway with clear (translucent) Silastic 734 RTV. The lung tissue was then corroded with potassium hydroxide.</p><p><strong>Results: </strong>Vascular-bronchoalveolar casts were recovered giving fine detail as shown using stereo light microscopy or scanning electron microscopy.</p><p><strong>Conclusions: </strong>This method may be useful for not only microvascular anatomy studies of lungs, but also for studying the microvasculature of other normal and diseased tissues.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"479-82"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430410","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19577380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of high dose of parathyroid hormone on fetal osteoclasts and their precursors in vivo: an ultrastructural-cytochemical study.","authors":"H Isaki,&nbsp;H Hanaoka","doi":"10.1002/ar.1092430404","DOIUrl":"https://doi.org/10.1002/ar.1092430404","url":null,"abstract":"<p><strong>Background: </strong>It is not well known how the immediate precursors of osteoclast develop into osteoclasts in the fetus. This ultrastructural-cytochemical study was designed to clarify the formation process of the osteoclasts and their increased activities in the fetal mouse limb buds after administration of high dose parathyroid hormone (PTH).</p><p><strong>Methods: </strong>Twenty-four or forty-eight hours after the high doses of PTH were injected into amniotic fluid of the pregnant C3H mice, the femoral limb buds of embryos were dissected out. Tartrate-resistant acid phosphatase (TRAP) reactions were performed while preparing specimens for electron microscopy.</p><p><strong>Results: </strong>Both control and PTH-given preosteoclasts and osteoclasts exhibited TRAP-positivities in dense bodies and vesicles. As effects of PTH, a binucleated preosteoclast of tandem fashion was observed. More osteoclastic hyperactivities were observed in the diaphyseal bone marrow. An osteoclast with a large cytoplasm exhibited two sets of clear zones and ruffled borders. Some osteoclasts demonstrated prominent amoeboid figures, while other osteoclasts developed large cytoplasmic vacuoles, which contained pieces of calcified chondroid bars.</p><p><strong>Conclusions: </strong>Our results revealed the progression of maturation from young preosteoclasts to osteoclasts. An existence of a peculiar binucleated preosteoclasts suggested one of the processes for multinucleation of the osteoclast. Quite remarkable osteoclastic hyperactivities were obviously the effects of high dose PTH. Our results also indicated the endophagocytic ability of the osteoclast. How PTH affected the osteoclasts and their precursors in the diaphyseal bone marrow can be speculated.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"421-9"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19577428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative morphology and cytology of the olfactory organs in Moray eels with remarks on their foraging behavior.","authors":"L Fishelson","doi":"10.1002/ar.1092430402","DOIUrl":"https://doi.org/10.1002/ar.1092430402","url":null,"abstract":"<p><strong>Background: </strong>This study compares the morphology and cytology of olfactory organs in moray eels (Muraenidae), particularly Siderea grisea and some species of the genera Echidna, Gymnothorax, and Lycodontis, fishes that are top predators in shallow-water marine habitats. Some of the species search visually for food while others search by olfaction.</p><p><strong>Methods: </strong>The morays were collected in the Red Sea; the nasal olfactory organs were dissected and fixed in Bouin's solution for light-microscopy, and 3.5% glutaraldehyde for electron-microscopy studies.</p><p><strong>Results: </strong>In each studied species the olfactory rosettes are elongated structures situated in closed olfactory chambers between anterior tubular inlet nares and slit-form posterior outlet openings. The double row of lamellae constituting these rosettes are round in Siderea and Echidna and elongated in the other species. They are attached at their base to a median raphe and range in number from 20 in the youngest observed Siderea to 168 in Gymnothorax of 1,500 mm total length. As in other teleosts, the lamellae are covered by a ciliated epithelium composed of three types of sensory cells: two of these, ciliated sensory neurons and ciliated supporting cells, differ in detail, length, and thickness of their cilia and intracellular rootlet system; the third type of sensory cells bears microvillae as well as cilia. Proximal, axonal extensions of the ciliated cells cross the basal lamina in bundles and combine to form fila olfactoria from which the two olfactory nerves extend towards the olfactory bulbs. Lateral extensions at the basal parts of these ciliated cells, the so-called spines, cross the membranes of neighboring cells as dendrites, possibly changing part of all of the ciliated epithelium into an olfactory field. The density and number of sensory cells on the lamellae, as well as observed differences in their foraging behavior in nature and captivity, enable the morays to be divided into two groups: one group, in which the lamellae are richly covered with stereocilia, includes species of the genera Siderea and Echidna, that search for food by olfaction, and the second group, which has a great deal less cells with stereocilia and includes the studies species of Gymnothorax and Lycodontis, locates its food visually.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"403-12"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430402","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19577426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological changes in the normal pattern of ventricular myoarchitecture in the developing human heart.","authors":"D Sanchez-Quintana,&nbsp;V Garcia-Martinez,&nbsp;V Climent,&nbsp;J M Hurle","doi":"10.1002/ar.1092430411","DOIUrl":"https://doi.org/10.1002/ar.1092430411","url":null,"abstract":"<p><strong>Background: </strong>The aim of the present study was to describe the morphological changes in the normal pattern of ventricular myoarchitecture in the prenatal and adult human heart, to understand the three-dimensional organization of the muscle fibers and their active functional role in valvular dynamics.</p><p><strong>Methods: </strong>We used dissection and histological techniques in 56 human hearts from fetuses and adults of both sexes.</p><p><strong>Results: </strong>In all hearts, the ventricular wall was arranged in three different layers: superficial (subepicardial), middle, and deep (subendocardial) myocardium. The superficial and deep layers are present in both ventricles, whereas the middle layer is found only in the left ventricle. Age-related differences were noted in the pattern of myoarchitecture of the superficial layer, mainly in the fetal period, and especially in the right ventricle; however, the middle layer always shows a circumferential pattern, which is specially evident in elderly hearts. The ventricular fibers in the superficial and deep layers are anchored in the ventricular orifices.</p><p><strong>Conclusions: </strong>Our findings reveal that muscle fiber architecture showed age- but not sex-related differences. These variations may reflect a mechanism of adaptation of the heart to functional demands throughout life.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"483-95"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19577381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}