Annals of Diagnostic Pathology最新文献

{"title":"Variability in primary thyroid lymphoma: A clinicopathological exploration of diffuse large B-cell, marginal zone, and follicular lymphoma","authors":"Agnes Stephanie Harahap ,&nbsp;Ivana Santoso ,&nbsp;Stefanny Charles ,&nbsp;Amanda Virginia Hapsari Ardhiawan ,&nbsp;Abdillah Hasbi Assadyk ,&nbsp;Maria Francisca Ham","doi":"10.1016/j.anndiagpath.2025.152444","DOIUrl":"10.1016/j.anndiagpath.2025.152444","url":null,"abstract":"<div><div>Primary thyroid lymphoma (PTL) is a rare condition, posing significant diagnostic challenges due to limited incidence and data. However timely and accurate diagnosis is crucial for effective management. This study aims to analyze the clinicopathological features of PTL cases observed over 15 years at a tertiary national referral hospital. PTL cases from 2009 to 2023 at Universitas Indonesia – Dr. Cipto Mangunkusumo Hospital archives were retrospectively analyzed, with an assessment of clinical data, histopathological, and immunohistochemistry analysis. Statistical analysis was conducted using Chi-Square and Kruskal Wallis. Women constituted the majority of cases (male-to-female ratio was 1: 2.6), with a median patient age of 55 years. Of the 40 identified PTL cases, only one was a T-cell lymphoma among the non-Hodgkin lymphomas (NHL). The NHL subtypes included diffuse large B-cell lymphoma (DLBCL [72.5 %]), marginal zone lymphoma (15.0 %), and follicular lymphoma (FL [10.0 %]). An enlarged neck mass (94.7 %) was the most frequent symptom, and 42.1 % had a history of Hashimoto's thyroiditis. The overall surviving proportion in the present study is 80.7 %, with the median survival duration of 14.5 months, ranging from 1 to 54 months. The longest duration of survival documented in FL case and the shortest in DLBCL case. Lymphoepithelial lesions could be found in all lymphoma types. The main diagnostic and treatment modality used was surgery. Prompt diagnosis and personalized treatment approaches are important to improve survival outcomes. PTL should be anticipated in middle-aged women with rapid enlarged neck mass and a history of Hashimoto's thyroiditis.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152444"},"PeriodicalIF":1.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital image analysis of tumour pattern and histological models for prognostic evaluation of invasive non-mucinous adenocarcinoma of the lung","authors":"Waratchaya Tirasarnvong,&nbsp;Kanet Kanjanapradit","doi":"10.1016/j.anndiagpath.2025.152445","DOIUrl":"10.1016/j.anndiagpath.2025.152445","url":null,"abstract":"<div><div>The 2021 World Health Organisation classification of lung adenocarcinoma is based on the predominance and percentage of high-grade histological patterns, e.g. solid and micropapillary patterns, determined by semiquantitative estimation. Digital pathology can be used to evaluate the area of each pattern and calculate the exact percentage. To evaluate the prognostic predictive ability of a histological model for invasive non-mucinous adenocarcinoma using digital pathology. This retrospective cohort study included 76 patients with invasive non-mucinous lung adenocarcinoma who underwent lung resection at Songklanagarind Hospital between January 2010 and December 2016. The histological pattern area was measured on a digital slide using the QuPath Open software version 0.3.2. Clinical and pathological data, including the presence of tumour spread through airspaces, tumour necrosis, tumour-infiltrating lymphocytes, and lymphovascular invasion, were collected. The primary outcome was 5-year overall survival. The best model was provided by the Akaike information criterion, and the prognostic discrimination ability was compared with that of other models from previous studies by identifying the area under the curve (AUC) in the receiver operating characteristic analysis. The best model was validated using bootstrapping. The best model was a combination of stage and an 82 % cut-off high-grade pattern (AUC = 0.776). Tumours with ≥82 % high-grade pattern resulted in significantly worse prognoses (<em>p</em> = 0.001) than those with &lt;82 % high-grade pattern. Our model had the highest AUC among all models from previous studies. This was validated using bootstrapping, with an AUC of 0.708. The best model for survival prediction was a combination of stage and an 82 % cut-off high-grade pattern.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152445"},"PeriodicalIF":1.5,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GPNMB expression differentiates subependymal giant cell astrocytoma from other mimickers","authors":"Rui Pan,&nbsp;Xiaotong Wang,&nbsp;Ru Fang,&nbsp;Qiuyuan Xia,&nbsp;Xuan Wang,&nbsp;Rusong Zhang,&nbsp;Xue Wei,&nbsp;Nan Wu,&nbsp;Qiu Rao","doi":"10.1016/j.anndiagpath.2025.152442","DOIUrl":"10.1016/j.anndiagpath.2025.152442","url":null,"abstract":"<div><div>Subependymal giant cell astrocytomas (SEGAs) are neoplasms that exhibit slow growth patterns and are closely associated with tuberous sclerosis complex (TSC). Recent research indicates that TFE3/TFEB-targeted biomarker glycoprotein nonmetastatic B (GPNMB) is upregulated inTSC1/2-related tumours. In this study, we performed molecular analysis on SEGAs and analyzed GPNMB expression in 6 SEGAs, 10 PXAs, 9 GBMs, 8 eGBMs, 8 diffuse astrocytomas, 8 oligodendrogliomas and 7 glioneuronal tumours through immunohistochemistry, 100 % (6/6) of the SEGA cases exhibited positive GPNMB expression, whereas it was negative in all other CNS tumours. The results indicated that GPNMB is specifically expressed in all SEGAs, distinguishing it from other morphologically similar tumours. The findings demonstrate specific GPNMB expression in SEGA, underscoring its promise as a diagnostic biomarker.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152442"},"PeriodicalIF":1.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the predictive value of Ki-67 and progesterone receptor algorithms for recurrence and disease-free survival in meningiomas","authors":"Servet Guresci ,&nbsp;Ozge Basaran Aydogdu ,&nbsp;Ahmet Eren Secen ,&nbsp;Burak Uzel","doi":"10.1016/j.anndiagpath.2025.152441","DOIUrl":"10.1016/j.anndiagpath.2025.152441","url":null,"abstract":"<div><div>Sophisticated refinements in histopathology are evolving to improve meningioma outcome prediction. The aim of this study is to evaluate the stand-alone performance of Ki-67 and progesterone receptor (PR) algorithm scores in meningiomas and their power in predicting recurrence and disease-free survival of the patients.</div><div>Whole slide images of Ki-67 and PR-stained slides from 404 meningioma cases were analyzed by a digital image viewer and analysis software Virapath-2.1, which analyzes the tumor cells by size, color, and shape. Ki-67 scores were calculated in the hotspot region that contains at least 1000 tumor cells, while PR was calculated on the whole slide. The results were compared with WHO grade, tumor recurrence and disease-free survival (DFS).</div><div>Mean Ki-67 scores were 4.2 ± 3.5, 12.1 ± 10.6 and 22. ± 8.5 for grade 1, 2 and 3 tumors (p &lt; 0.05), while PR scores were 49 ± 35, 43 ± 34 and 16 ± 30, respectively (p &gt; 0.05). Median survival of patients based on Ki-67 values ≤13.2 % and &gt; 13.2 % was 122 versus 60 months (p = 0.004). Prediction of recurrence based on Ki-67 score was found to have acceptable discrimination (AUC = 0.74). PR expression was not found to correlate with DFS, but recurrent tumors had lower PR scores than non-recurrent tumors (31.3 ± 33.8 vs. 49.0 ± 33.0; p = 0.03).</div><div>Elevated Ki-67 levels identified by the algorithm may classify meningioma patients at high recurrence risk and inform clinical management. Although PR scores did not correlate with DFS, lower expression in recurrent tumors suggests a role in recurrence risk assessment. Larger prospective studies are needed for routine clinical practice.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152441"},"PeriodicalIF":1.5,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological and prognostic significance of stromal p16 and p53 expression in oral squamous cell carcinoma","authors":"Yusuke Amano ,&nbsp;Masayo Hasegawa ,&nbsp;Atsushi Kihara ,&nbsp;Daisuke Matsubara ,&nbsp;Noriyoshi Fukushima ,&nbsp;Hiroshi Nishino ,&nbsp;Yoshiyuki Mori ,&nbsp;Kentaro Inamura ,&nbsp;Toshiro Niki","doi":"10.1016/j.anndiagpath.2025.152439","DOIUrl":"10.1016/j.anndiagpath.2025.152439","url":null,"abstract":"<div><div>The tumor microenvironment is highly heterogeneous and consists of neoplastic cells and diverse stromal components, including fibroblasts, endothelial cells, pericytes, immune cells, local and bone marrow-derived stromal stem and progenitor cells, and the surrounding extracellular matrix. Although the significance of p16 and p53 has been reported in various tumor types, their involvement in the stromal cells of oral squamous cell carcinoma (OSCC) remains unclear. We performed immunohistochemical analyses of p16 and p53 expression in OSCC samples, Of the 116 samples, 74 showed p16-positive stromal cells, and 33 showed p53-positive stromal cells. Both p16 and p53 positivity were associated with an increased histological grade, lymphovascular invasion, an immature stromal pattern with abundant amorphous extracellular matrix material, infiltrative invasion patterns (Yamamoto Kohama classification-4C and <img>4D), and poor prognosis. Multivariate analyses identified p16 and p53 positivity in the stroma as independent prognostic factors for overall survival (<em>P</em> = 0.032 and <em>P</em> = 0.020, respectively); moreover, stromal p16 positivity correlated with stromal p53 positivity. These findings indicated that p16 and p53 stroma positivity may regulate OSCC tumor aggressiveness.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152439"},"PeriodicalIF":1.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gamna-Gandy bodies in renal neoplasms: A multi-institutional clinicopathologic study of 350 consecutive nephrectomies","authors":"Aysha Mubeen ,&nbsp;Richard Pacheco ,&nbsp;Mahmut Akgul ,&nbsp;Sean R. Williamson ,&nbsp;Katrina Collins ,&nbsp;Emily Chan ,&nbsp;Ankur R. Sangoi","doi":"10.1016/j.anndiagpath.2025.152440","DOIUrl":"10.1016/j.anndiagpath.2025.152440","url":null,"abstract":"<div><div>Gamna-Gandy (GG) bodies are sclerosiderotic nodules composed of iron pigment and calcium, that have been described predominantly in the spleens of patients with sickle cell disease. Their formal depiction in the kidney is mainly limited to case reports and small series. We aimed to investigate the incidence of GG bodies and associated clinicopathologic features in consecutive nephrectomies performed for renal tumors. Slides of consecutive nephrectomies for renal neoplasms at 3 institutions were reviewed by genitourinary pathologists, with detailed clinicopathologic features recorded. The incidence of GG bodies in our nephrectomy cohort was 13% (44/350). The most common tumor exhibiting GG bodies was clear cell renal cell carcinoma (RCC) (40/44), followed by papillary RCC (2/44), chromophobe RCC (1/44), and epithelioid angiomyolipoma (1/44). Most RCCs were pathologic stage pT3a (46%). GG bodies were intratumoral in 77%, peritumoral in 5%, and both in 18% of patients. They were focal in 43% and multifocal in 57%, with the largest focus ranging from 0.2 to 7 mm (mean 1.7 mm). Background fibrosis and hemosiderin laden macrophages were commonly associated (93% for both). All tumors demonstrated cystic elements. In 2 renal tumor specimens, an extensive fungal workup was performed and was negative; the “structures” were not formally recognized as GG bodies in either specimen. GG bodies are not an uncommon finding in renal tumors, particularly in clear cell RCC. Awareness of the morphologic appearance is crucial to avoid mistaking them for fungal structures or other organisms.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152440"},"PeriodicalIF":1.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new histomorphological finding in the follow-up of celiac disease: Intraepithelial lymphocyte localization is a reliable indicator of dietary compliance","authors":"F. Yilmaz ,&nbsp;K. Atay","doi":"10.1016/j.anndiagpath.2025.152438","DOIUrl":"10.1016/j.anndiagpath.2025.152438","url":null,"abstract":"<div><div>The correlation between clinical, serological, and endoscopic findings and histological response after a gluten-free diet (GFD) is limited in adult celiac (CD) patients. This study aims to evaluate the effects of GFD on intraepithelial lymphocyte (IEL) localization by comparing the histopathological, clinical, serological, and endoscopic findings of adult CD patients. The patients (<em>n</em> = 131) were divided into three groups: those with good (CDgc) (<em>n</em> = 23) and poor (CDpc) (<em>n</em> = 21) GFD compliance and newly diagnosed ones (nCD) (<em>n</em> = 87). Total and supranuclear IELs were counted per 100 enterocytes and divided into three groups: apical, mixed, and basal, according to ROC (Receiver operating characteristic) analysis. The roles of clinicopathological parameters in predicting good dietary compliance were calculated using the multivariable logistic regression model. CDgc group predominantly (78.3 %) exhibited a basal pattern, and none exhibited an apical. Conversely, most CDpc and nCD patients showed mixed (66.7 % and 73.6 %, respectively) and apical (9.5 % and 25.3 %) patterns. Non-atrophic Marsh types (<em>p</em> = 0.040) and basal pattern (<em>p</em> = 0.043) were independent parameters predicting good dietary compliance. This study first showed that IEL localizations can indicate GFD compliance in samples from CD patients. Localization-based examination of IELs can be an additional histological indicator in monitoring GFD compliance and signs of recovery, especially in adult CD patients.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152438"},"PeriodicalIF":1.5,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphologic and immunohistochemical characterization of small and large duct cholangiocarcinomas in a Western cohort: A panel of pCEA, CRP, N-cadherin, and albumin in situ hybridization aids in subclassification","authors":"Raymond Gong ,&nbsp;Zongming E. Chen ,&nbsp;Karen Matsukuma","doi":"10.1016/j.anndiagpath.2025.152437","DOIUrl":"10.1016/j.anndiagpath.2025.152437","url":null,"abstract":"<div><div>Two morphologic subtypes of intrahepatic cholangiocarcinoma (iCCA), small duct and large duct, are now recognized, and importantly, these subtypes are associated with distinct molecular pathways and therapeutic options. Initial studies demonstrated the feasibility of morphologic subclassification and helped characterize the immunoprofile of the subtypes. However, few studies have been undertaken in Western countries where incidence of the subtypes is likely distinct from that in the East. To address this, 48 tumors from a North American cohort, consisting of 29 iCCAs, 18 extrahepatic CCAs (eCCAs), and 1 tumor of unclear origin (liver vs. gallbladder growing into liver) were classified by morphologic criteria as large duct (19 tumors), small duct (13 tumors), or indeterminate (13 tumors; all iCCAs). Notably, only 3 iCCAs were classified as large duct. Additionally, we evaluated the utility of common biomarkers to aid in subclassification, given that a significant portion of iCCAs were challenging to classify (e.g., indeterminate morphology). Tumors were screened for expression of mucicarmine, epithelial membrane antigen (EMA), monoclonal (mCEA), polyclonal CEA (pCEA), N-cadherin, CD56, and albumin by in situ hybridization (ALB-ISH). Of these, pCEA, CRP, N-cadherin, and ALB-ISH showed statistically significant differences between large and small duct types (<em>P</em> &lt; 0.0028), with high specificity (≥88 %) and at least moderate sensitivity (≥60 %). Eleven of the 13 morphologically indeterminate tumors could be classified based on their expression of these 4 markers. Four additional large duct iCCAs were subsequently obtained from a second North American institution and assessed for pCEA, N-cadherin, and albumin expression. Combining these data with the initial cohort of large duct iCCAs (total of 7 large duct iCCAs) showed similar biomarker associations. In conclusion, in this Western cohort, 55 % of iCCAs (16 of 29) could be subclassified as large or small duct type based on morphology alone. With the aid of the 4-marker panel, 93 % of iCCAs (27 of 29) could be classified. Unlike in East Asian cohorts, the vast majority of iCCAs (88 %) was small duct type, and hepatolithiasis was not observed. CRP, N-cadherin, and ALB-ISH were found to be specific for small duct iCCA, whereas diffuse, strong expression of pCEA showed specificity for large duct tumors. This is the first report to highlight the utility of pCEA to subclassify iCCAs. Additionally, in cases in which the primary site (within the biliary tract) was unclear, CRP, ALB-ISH, N-cadherin, and pCEA were helpful in distinguishing iCCA from eCCA.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152437"},"PeriodicalIF":1.5,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary intrarenal hemangioma – A series of 39 cases","authors":"Maria Faraz ,&nbsp;Andrew Rosenzweig ,&nbsp;Angel Panizo ,&nbsp;Sabina Hajiyeva ,&nbsp;Nusret B. Subasi ,&nbsp;Mohammed A. Alghamdi ,&nbsp;Andrea A. Lightle ,&nbsp;Levente Kuthi ,&nbsp;Dora Kelemen ,&nbsp;Ankur R. Sangoi ,&nbsp;Luiz M. Nova-Camacho ,&nbsp;María Garcia Martos ,&nbsp;Mehrnaz Movassaghi ,&nbsp;Anandi Lobo ,&nbsp;Shilpy Jha ,&nbsp;Kutsal Yörükoğlu ,&nbsp;Busra Yaprak Bayrak ,&nbsp;Sean R. Williamson ,&nbsp;Swati Bhardwaj ,&nbsp;Shivani Kandukuri ,&nbsp;Mahmut Akgul","doi":"10.1016/j.anndiagpath.2025.152436","DOIUrl":"10.1016/j.anndiagpath.2025.152436","url":null,"abstract":"<div><div>Intrarenal hemangiomas lack concise clinicopathologic information, due to the predominance of single case reports and inclusion of other vascular neoplasms and hemangiomas of perirenal, hilar, and renal vein origin. Herein, in this multi-institutional study we evaluate clinicopathologic features of 39 intrarenal hemangiomas. The median age was 62 years (range = 27–94 years; 2:1 male to female ratio), with left-sided predominance (left = 21, right = 13; one case was bilateral). The median tumor size was 1.5 cm (0.2-10 cm). Two cases arose from transplanted kidneys. Most were asymptomatic (<em>n</em> = 30, 86 %), even though most surgical interventions (19 partial, 19 radical, 1 biopsy) were due to hemangiomas (<em>n</em> = 24, 62 %). Synchronous renal neoplasms were present in 9 (23 %) patients, including clear cell renal cell carcinoma (RCC) (<em>n</em> = 4), angiomyolipoma (n = 2), oncocytoma (n = 2), and chromophobe RCC (<em>n</em> = 1). Multifocal hemangiomas (<em>n</em> = 5) were seen in cases with end stage renal disease. Intrarenal hemangiomas were mostly anastomosing (<em>n</em> = 18; 46 %), followed by capillary (<em>n</em> = 15; 38 %), and cavernous (<em>n</em> = 6; 16 %) subtypes. Fibrin thrombus (<em>n</em> = 9; 23 %) and extramedullary hematopoiesis (<em>n</em> = 4; 10 %) were occasionally present, the latter being only in the anastomosing subtype. Immunohistochemistry was performed on a majority (<em>n</em> = 33, 84 %) of hemangiomas, with vascular markers CD31 and CD34 and lack of PAX8 were most used for diagnosis. 30 patients had follow-up (median 48 months, range 1–241 months), none showed disease progression/recurrence. This study provides comprehensive observation of the largest intrarenal hemangioma cohort, highlighting their frequent cause of surgical intervention when present, predominance of anastomosing subtype, multifocality in end stage kidney disease, and occasional concurrent ipsilateral neoplasms.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152436"},"PeriodicalIF":1.5,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142967150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical markers of potential utility in identifying POLE-mutant endometrial carcinomas: An assessment of autocrine motility factor (AMF) and autocrine motility factor receptor (AMFR)","authors":"Anıl Alpsoy ,&nbsp;Gözde Koca Yılmaz ,&nbsp;Ceyda Karadağ ,&nbsp;Özer Birge ,&nbsp;Tayup Şimşek ,&nbsp;Gülgün Erdoğan ,&nbsp;Hadice Elif Peştereli","doi":"10.1016/j.anndiagpath.2024.152433","DOIUrl":"10.1016/j.anndiagpath.2024.152433","url":null,"abstract":"<div><div><em>POLE</em> status determination is necessary for the molecular classification of endometrial carcinomas (EC). However, this determination is only achievable by molecular techniques, which are not available in many practice settings. A previously published study reported elevated AMF/GPI and AMFR/gp78 levels in <em>POLE</em>-mutant EC. We examined the relationship between <em>POLE</em> status and AMF and AMFR expression. Our study included 55 molecularly classified EC, assessed for AMF and AMFR immunohistochemically. Staining intensity was scored 0 (negative), 1 (weak), 2 (medium), 3 (strong), extent was scored 0 (0 %), 1 (1–25 %), 2 (26–50 %), 3 (51–75 %), 4 (76–100 %), with those parameters summed for the final score for each case. The molecular subtypes <em>POLE</em> mutant, mismatch repair-deficient, no specific molecular profile, p53 abnormal had mean AMF scores of 5.909, 4.643, 5.000, 4.667, respectively. The <em>POLE</em>-mutant subtype had a significantly higher average AMF score than <em>POLE</em> wild-type (<em>POLEwt</em>) group (<em>p</em> = 0.003). Using <em>POLE</em> mutant status as an end-point, ROC analysis showed that an AMF immunohistochemical score of 6 and above had an 81.8 % sensitivity, 61.4 % specificity, AUC of 0.708 (95 % CI, 0.565–0.851). <em>POLE</em>-mutant subtype had higher prevalence of a score of 6 and above than the <em>POLEwt</em> group (9/11 vs 17/44 cases, <em>p</em> = 0.010). An AMF score 6 and above increased the likelihood of being <em>POLE</em>-mutant by a factor of 10.496 (95 % CI, 1.592–69.212). Similarly, the <em>POLE</em>-mutant subtype had higher prevalence of AMFR scores of 5 and above than the <em>POLEwt</em> group (<em>p</em> = 0.023). AMF may offer some promise in identifying <em>POLE</em>-mutant EC with relatively high sensitivity, but suboptimal specificity indicates that it cannot be applied alone in practice. Additional studies are required to determine whether AMF can be combined with other markers to more optimally identify <em>POLE</em> mutant EC.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"75 ","pages":"Article 152433"},"PeriodicalIF":1.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}