Annals of Diagnostic Pathology最新文献

{"title":"Diagnostic value of cyclin D1 immunohistochemistry in differentiating malignant mesothelioma from reactive mesothelial proliferation","authors":"Döndü Nergiz ,&nbsp;Arsenal Sezgin Alikanoğlu ,&nbsp;Dinç Süren","doi":"10.1016/j.anndiagpath.2025.152502","DOIUrl":"10.1016/j.anndiagpath.2025.152502","url":null,"abstract":"<div><h3>Introduction</h3><div>Although morphologic assessment carries utmost importance in differentiating malignant mesotheliomas (MM) from reactive mesothelial proliferations (RMP), sometimes it may fail to clearly differentiate between these two entities. The aim of this study is to evaluate the potential role of cyclin D1 immunohistochemistry in this differentiation.</div></div><div><h3>Material and methods</h3><div>Eighty cases (40 MM, 40 RMP) were examined. Nuclear staining of the mesothelial cells was evaluated after cyclin D1 immunostaining. Immunostaining was scored according to the percentage of immunopositive cells (0 %, 1–25 %; 26–50 %; 51–75 %; 76–100 %).</div></div><div><h3>Results</h3><div>For MMs, 24/40 (60 %) cases demonstrated &gt;50 % staining, with 13/24 in the 51–75 %, 11/24 in the &gt;75 % range. RMPs generally showed no staining (27/40 cases) or 1–25 % staining (11/40 cases) with no cases showing &gt;50 % staining. There was a statistically significant difference between RMP and MM groups according to Cyclin D1 staining percentage (<em>p</em> = 0.001). When 50 % was set as the cut-off value for Cyclin D1 staining, cyclin D1 immunohistochemistry had 60 % sensitivity, 100 % specificity and 80 % accuracy in differential diagnosis between MM and RMP.</div></div><div><h3>Conclusion</h3><div>In the diagnosis of MM, immunostaining of &gt;50 % of the cells with cyclin D1 is a useful adjunct to morphologic assessment. Although cyclin D1 immunostaining showed high specificity when 50 % immunopositivity was set as a cut-off value in the differentiation between MM and RMP, its sensitivity and accuracy were relatively low. Validation of diagnostic utility of cyclin D1 immunohistochemistry based on the results of relevant prospective studies is necessary before its clinical application.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152502"},"PeriodicalIF":1.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rethinking tissue preservation: A review of non-toxic and environmentally sustainable fixatives","authors":"Sowmiya Shri Thangamani,&nbsp;Swaathi Ravindran,&nbsp;Magesh Karuppur Thiagarajan,&nbsp;Sathya Kumar Mayilvakanam,&nbsp;Aravindhan Ravi,&nbsp;Sivachandran Annamalai","doi":"10.1016/j.anndiagpath.2025.152500","DOIUrl":"10.1016/j.anndiagpath.2025.152500","url":null,"abstract":"<div><div>Fixatives are substances used in histopathology to preserve tissue samples by preventing degradation and keeping them structurally intact for microscopic analysis. The common examples include formaldehyde, glutaraldehyde, and alcohols. The widely used fixative, formaldehyde, carries significant health hazards, like respiratory distress, mucosal irritation and carcinogenic effects. Consequently, there is a growing academic impetus to explore natural alternatives to traditional chemical fixatives. Studies were obtained from PubMed database using keywords that covered a wide range of fixatives, including natural, formalin-based, sugar-based, plant-based, and alternative options. Collected data was analyzed and organized into four categories of naturally available fixatives: <em>Aloe vera</em>, Honey, Jaggery, Sugar. 11 studies were included from January 2013 to March 2024. In these, the alternative fixatives has been used for tissue processing. Honey was tested eight times at concentrations of 10 %, 20 %, and 100 %; jaggery six times at 30 % and 100 %; Sugar was used as sugar syrup three times at 20 % and 100 %, as sirka once and as sugarcane vinegar in one study; <em>aloe vera</em> once at 20 %; ethanol twice at 95 %; and formalin nine times at 4 % and 10 %. Compelling evidence demonstrates that natural fixatives can provide tissue preservation on par with formalin, particularly in short-term applications. Although these fixatives are harmless, they come with certain disadvantages Honey has shorter shelf life; Jaggery cause mold formation and produces discoloration of sample; Sugar attracts insects and leads mold formation; <em>Aloe vera</em> produce poor nuclear staining and cell morphology preservation.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152500"},"PeriodicalIF":1.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sarcomatoid squamous cell carcinoma of the cervix: A case series and literature review","authors":"Qiuyang Jing ,&nbsp;Wenjuan Pu ,&nbsp;Peng Guo ,&nbsp;Xin Liao","doi":"10.1016/j.anndiagpath.2025.152501","DOIUrl":"10.1016/j.anndiagpath.2025.152501","url":null,"abstract":"<div><div>Primary cervical sarcomatoid squamous cell carcinoma (SSCC), a rare biphasic malignancy with 29 documented cases, is characterized by co-existing malignant squamous epithelium and sarcomatoid spindle cells. Immunohistochemical co-expression of epithelial (PCK) and mesenchymal (vimentin) markers remains diagnostic. We analyzed 8 institutional cases (2006–2024) alongside literature reports. Patients (median age 46, range 28–72) predominantly presented with vaginal bleeding (7/8 cases), while one exhibited abdominal pain with discharge. HPV16 was detected in 2 cases. All tumors showed variable immunoreactivity for PCK, vimentin, p16, and ≥ 1 squamous marker (p63/p40/CK5/6). Treatment included chemotherapy (6/8) and radiotherapy (3/8). Follow-up (median 57.4 months, <em>n</em> = 7) revealed aggressive behavior: two stage IIIC patients developed supraclavicular/visceral metastases (alive with disease at 10–31 months), one stage IIIA patient died at 24 months, while others remained disease-free. Combined analysis with 29 published cases confirms SSCC's dual histologic presentation (biphasic/monophasic) and consistent epithelial-mesenchymal marker co-expression. These findings underscore SSCC's clinicopathologic heterogeneity and metastatic potential despite multimodal therapy.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152501"},"PeriodicalIF":1.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Papillary renal neoplasm with reverse polarity shows benign behavior: Results from a 77-case clinicopathological and molecular study.","authors":"Yong Il Lee, Ja-Min Park, Sun Young Yoon, Cheryn Song, Yong Mee Cho","doi":"10.1016/j.anndiagpath.2025.152498","DOIUrl":"https://doi.org/10.1016/j.anndiagpath.2025.152498","url":null,"abstract":"<p><p>Papillary renal neoplasm with reverse polarity (PRNRP) is a low-grade renal tumor characterized by oncocytic cells with apical nuclei, GATA3 expression, and frequent KRAS mutations. This study aimed to comprehensively characterize and determine prognostic significance of PRNRP by conducting clinical, histological, immunohistochemical, and genetic analyses while reviewing prior reports. A total of 506 patients with papillary renal cell carcinoma (PRCC) who underwent curative nephrectomy from 1989 to 2023 were analyzed, leading to the reclassification of 77 cases (17 %) as PRNRP. All cases were pT1 tumors with a median size of 1.5 cm. Initially, 40 %, 31 %, and 18 % were diagnosed as PRCC type 1, PRNRP, and PRCC type 2, respectively. PRNRP cases consistently displayed characteristic features; however, two tumors exhibited high-grade nuclear atypia resembling WHO/ISUP grade 3, highlighting the existence of atypical subgroups. Immunohistochemical analysis showed GATA3 expression in 97 % of cases. Transcription factor EB (TFEB) expression was observed in 86 % of cases. KRAS exon 2 mutations were identified in all cases, including the two atypical PRNRPs. Neither recurrence nor disease-specific death occurred throughout a median follow-up period of 46 months. This study highlights the necessity of thorough diagnostic evaluation, incorporating unique histological features, GATA3 positivity, and KRAS mutation analysis, especially in the presence of atypical morphology, while reaffirming the benign nature of PRNRP.</p>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":" ","pages":"152498"},"PeriodicalIF":1.5,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retroperitoneal schwannoma: A clinicopathological analysis of 14 cases","authors":"Shuting Li ,&nbsp;Zhenyong Wu ,&nbsp;Xiaodong Lin ,&nbsp;Lifang Ye ,&nbsp;Xin Zeng","doi":"10.1016/j.anndiagpath.2025.152497","DOIUrl":"10.1016/j.anndiagpath.2025.152497","url":null,"abstract":"<div><div>Retroperitoneal schwannomas (RSs) are rare benign neurogenic tumors with nonspecific imaging features and histological mimics, posing significant diagnostic challenges. To address this gap, we retrospectively analyzed 14 RS cases to delineate clinicopathological and immunohistochemical characteristics. Cases were retrieved from the pathology database of the First Affiliated Hospital of Guangzhou Medical University (2019–2024), with hematoxylin-eosin (H&amp;E) and immunohistochemical (IHC) staining performed for evaluation. Among 14 patients, the male/female ratio was 2:5 and the average age was 47.3 years (range, 28 to 71 years). Patients with RSs generally did not have specific symptoms, and they were usually found during routine medical examinations (9/14). All underwent total tumor resection, with a mean tumor size of 5.5 cm (range, 2.5 to 9.5 cm). Microscopically, typical schwannoma features were observed alongside degenerative changes, including cyst formation (6/14), hemorrhage (8/14), calcification (3/14), ossification (3/14), ossification with bone marrow elements (1/14), and degenerative nuclear atypia (5/14). Immunohistochemically, tumors were strongly positive for S-100 (12/12) and SOX10 (12/12), with focal EMA (1/3) and SMA (2/10) reactivity. Over a mean follow-up of 26.4 months (range, 2 to 68 months), 13 patients remained recurrence-free, while one experienced local recurrence after 43 months. Our findings highlight that RS diagnosis requires integrating clinical, radiological, morphological, and immunohistochemical analyses to distinguish these tumors from mimics. Despite their benign nature, long-term surveillance is advised due to the potential for recurrence. This study underscores the importance of a multidisciplinary approach in diagnosing and managing RSs.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152497"},"PeriodicalIF":1.5,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of storage temperature on nucleic acid quality in formalin-fixed paraffin-embedded tissue samples","authors":"Yoichiro Okubo ,&nbsp;Nagisa Toyama ,&nbsp;Rika Kasajima ,&nbsp;Haruya Saji ,&nbsp;Hiroyuki Ito ,&nbsp;Yui Kaburaki ,&nbsp;Chie Hasegawa ,&nbsp;Shu Yuguchi ,&nbsp;Emi Yoshioka ,&nbsp;Kota Washimi ,&nbsp;Shinya Sato ,&nbsp;Yukihiko Hiroshima ,&nbsp;Hiroyuki Hayashi ,&nbsp;Yohei Miyagi ,&nbsp;Tomoyuki Yokose","doi":"10.1016/j.anndiagpath.2025.152496","DOIUrl":"10.1016/j.anndiagpath.2025.152496","url":null,"abstract":"<div><div>Formalin-fixed paraffin-embedded (FFPE) tissue samples are widely used for genomic analyses in clinical and research settings. However, prolonged storage at room temperature may compromise nucleic acid quality, potentially affecting the reliability of molecular testing. In this study, we investigated the impact of various storage temperatures and repeated temperature changes on nucleic acid quality in FFPE samples. Four surgically resected tumor cases were analyzed. Following cold ischemia times of 29–60 min, tumor tissues were sampled upon arrival at the pathology department, divided into five equal parts, and fixed in 10 % neutral buffered formalin. The resulting FFPE blocks were stored at 18 °C, 4 °C, −20 °C, −80 °C, or − 150 °C. After storage, the blocks were returned to room temperature for sectioning and analysis. DNA and RNA quality were assessed at 3, 6, and 12 months using DNA integrity number (DIN), short-to-long cycle threshold (S/L Ct) ratio, RNA integrity number (RIN), and DV200. Samples stored at 18 °C and 4 °C exhibited time-dependent deterioration in certain indicators, whereas those stored at −20 °C or below maintained stable nucleic acid quality. Despite multiple freeze-thaw cycles, no significant degradation was observed under colder conditions. Our findings suggest that −20 °C storage is a feasible and effective approach for preserving nucleic acid integrity in FFPE tissues. Although limited by a small sample size (<em>n</em> = 4), this study provides practical insights into tissue archiving strategies and supports selective implementation of refrigerated storage for long-term molecular analysis.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152496"},"PeriodicalIF":1.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucinous neoplasms associated with mature cystic teratomas: A clinicopathologic study of 50 cases: Are we ready for nomenclature change?","authors":"Preetha Ramalingam ,&nbsp;Mario L. Marques-Piubelli ,&nbsp;Isabel Alvarado-Cabrero ,&nbsp;Maria Delia Perez Montiel ,&nbsp;Nidhi Tandon ,&nbsp;Elizabeth D. Euscher ,&nbsp;Anais Malpica","doi":"10.1016/j.anndiagpath.2025.152494","DOIUrl":"10.1016/j.anndiagpath.2025.152494","url":null,"abstract":"<div><div>Mucinous neoplasms associated with ovarian teratomas (MOvNTs) are uncommon with only a few large series published thus far. Their clinicopathologic features are evaluated in this multi-institutional study. Fifty MOvNTs were retrieved from the files of four institutions over 16-years (yr). Patient age, CEA and CA-125 levels, laterality, tumor size, type of mucinous tumor (according to gynecologic pathology criteria and proposed appendiceal nomenclature when applicable), immunohistochemistry (IHC), FIGO stage, presence of pseudomyxoma ovarii and peritonei, treatment, follow-up in months (mos), and outcome were recorded. Patients ranged in age from 17 years to 74 years (mean 43, median, 42). Increased CA-125 levels correlated with mucinous carcinoma and mucinous borderline tumor (p=0.0270), and CEA correlated with mucinous carcinoma (p=0.0256). Mucinous neoplasms were either cystadenomas (16), borderline tumors (20) or carcinomas (14). Forty-one were Mullerian/surface epithelial type and 9 were appendiceal-like. Using appendiceal nomenclature: Seven were low grade mucinous appendiceal neoplasms and 2 high grade appendiceal neoplasms. IHC: 39 % were CK20/CDX2/SATB2 positive and 61 % were CK7 &gt; CK20 (+), and SATB2-. Almost all patients presented with FIGO stage I disease; except one each with FIGO II and IIIC disease. All patients had surgical resection, and 10 patients had adjuvant therapy. Recurrences occurred in 4 patients (range: 10–84 mos). At last follow-up (median 60 mos): 33 patients had no evidence of disease, 3 died of disease, 1 was alive with disease, 2 died of other causes, and 11 were lost to follow-up. MOvNTs are usually Mullerian/surface epithelial type and FIGO stage I. As the only patients with adverse outcome were those who had a diagnosis of mucinous carcinoma or microinvasive carcinoma, whether of appendiceal-like or Mullerian/surface epithelial type, using established gynecologic, rather than GI criteria for classifying all types of MOvNTs is recommended.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152494"},"PeriodicalIF":1.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone assisted telepathology: A review","authors":"Nadeem Tanveer ,&nbsp;Farhat Naz","doi":"10.1016/j.anndiagpath.2025.152493","DOIUrl":"10.1016/j.anndiagpath.2025.152493","url":null,"abstract":"<div><h3>Objectives</h3><div>Many practicing pathologists of the current generation have used smartphone-assisted telepathology at some point in time or the other. The use case may vary-second opinions, social media posts, teaching, and so on. This has largely been made possible by the recent improvements in smartphone cameras, better smartphone microscope adaptors, and faster internet. This review aims to provide insights into the different techniques, practical applications, limitations, and prospects of smartphone-assisted telepathology.</div></div><div><h3>Methods</h3><div>We provide a ready reference for all practicing pathologists who want to use smartphone-assisted telepathology more effectively. The review is based on literature available on MEDLINE and personal observations and experiences.</div></div><div><h3>Results</h3><div>Smartphone-assisted telepathology despite its limitations is here to stay. With the introduction of 5G internet, internet speeds are bound to improve. However, the extent of this improvement would ultimately decide how far the smartphone can disrupt the world of telepathology.</div></div><div><h3>Conclusion</h3><div>Learning to use the smartphone effectively for telepathology is important for all pathologists. It is a silent revolution that is slowly but surely changing the way pathologists interact with each other professionally.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152493"},"PeriodicalIF":1.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the 2022 Paris System on diagnostic accuracy and estimating the risk of high-grade malignancy in urine cytology","authors":"Anju Khairwa,&nbsp;Preeti Diwaker","doi":"10.1016/j.anndiagpath.2025.152495","DOIUrl":"10.1016/j.anndiagpath.2025.152495","url":null,"abstract":"<div><div>In 2016, The Paris System 1.0 (TPS 1.0) was introduced to overcome the problem of lack of a standardized reporting system and leading to diagnostic dilemmas for treating physicians. Following this, TPS 2.0 was introduced in 2022 to deal with certain limitations of TPS 1.0. Objectives of study: To evaluate the diagnostic accuracy and risk of malignancy (ROM) with risk of high-grade malignancy (ROHM) of urinary tract by of TPS 2.0 with TPS 1.0 and conventional reporting (CR) of urine cytology. Data were collected retrospectively from 2016 to 2023 from the departmental archives. The cases were reviewed and categorized as per TPS 2.0, TPS 1.0 and CR. Of 875 urine samples studied, 168 urine samples of patients with histology correlation were analyzed. TPS 2.0 had a maximum sensitivity of 64.2 % in Group A, highest specificity of 95.5, positive predictive value of 96.4 % and negative predictive value of 37.5 % in group B comparison to TPS 1.0 and CR. TPS 2.0 has significantly high sensitivity, and maximum receiver operating curve (ROC) with area under the curve (AUC) score (0.695) compared to TPS 1.0 and CR. TPS 2.0 predicted ROM for AUC, SHGUC and HGUC categories 67.6 %, 92 % and 96.8 %, whereas ROHM 26.5 %, 46.2 %, and 98.8 %, respectively. CR and TPS 1.0 predicted ROHM (95.2 % and 81.5 %), respectively. Implementation of TPS 2.0 revealed highest diagnostic accuracy and ROC AUC score for urinary tract malignancy, compared to TPS 1.0 and CR. Our study results highlight the impact of TPS 2.0 in urine cytology reporting.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152495"},"PeriodicalIF":1.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PD - L1 (SP263) expression correlates with pathological aggressive parameters in prostate cancer","authors":"Xiaopeng Zhuang ,&nbsp;Huiting Li ,&nbsp;Zhijie You ,&nbsp;Hui Cheng ,&nbsp;Guodong Guo ,&nbsp;Xin Chen ,&nbsp;Haijian Huang","doi":"10.1016/j.anndiagpath.2025.152481","DOIUrl":"10.1016/j.anndiagpath.2025.152481","url":null,"abstract":"<div><h3>Objective</h3><div>Research on the expression of PD-L1 (clone SP263) in prostate cancer (PC) is rare. This study aims to investigate PD-L1 (SP263) expression and its clinicopathological correlations in PC.</div></div><div><h3>Methods</h3><div>A total of 265 PC samples at our center from 2021 to 2024 were included in this study. The clinical information and pathological data were collected. Whole-slide immunohistochemical analysis of PD-L1 (SP263), p53, ERG, PTEN, HER-2 and Ki67 was performed on PC samples, including core biopsies, radical prostatectomies, transurethral resections of the prostate and metastases. Next-generation sequencing was performed in 17 patients and the status of <em>TP53</em>, <em>BRCA1/2</em> were analyzed. Associations were assessed between PD-L1 status and clinicopathological parameters (age, preoperative serum prostate-specific antigen [PSA], surgical margin, Gleason Group [GG], TNM stage, Ki-67, p53/<em>TP53</em>, <em>BRCA1/2</em>, survival outcomes, etc.) using chi-square, Mann-Whitney U and Kaplan-Meier analyses.</div></div><div><h3>Results</h3><div>PD-L1 positivity (10.2 %, 27/265) correlated with advanced age (<em>P</em> = 0.007), high GG (<em>P</em> = 0.019), T3/4 stage (<em>P</em> = 0.001), positive surgical margin (<em>P</em> = 0.004), aberrant p53/TP53 (<em>P</em> = 0.043), and elevated Ki-67 (<em>P</em> = 0.042). No associations were observed between these factors (serum PSA, N category, M category, PTEN, ERG, BRCA1/2, or survival outcomes) and PD - L1.</div></div><div><h3>Conclusion</h3><div>The expression of PD-L1 (SP263) is positively associated with pathologically aggressive parameters in PC. This finding implies the potential value of PD-L1 as a biomarker for risk stratification. Moreover, it indicates the possibility of using PD-L1 (SP263) and p53 expression to guide the combinational application of mutated p53 inhibitors and PD-1/PD-L1 antibodies in PC.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152481"},"PeriodicalIF":1.5,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}