Annals of Diagnostic Pathology最新文献

{"title":"Comparative analysis of MIB1 and SP6 antibodies for Ki-67 assessment in breast carcinoma with focus on the influence of molecular subtyping","authors":"Hristo Milev ,&nbsp;Ivan Ivanov","doi":"10.1016/j.anndiagpath.2025.152523","DOIUrl":"10.1016/j.anndiagpath.2025.152523","url":null,"abstract":"<div><div>Ki-67 is a nuclear protein used as a proliferation marker in breast carcinoma, aiding in tumor classification and prognostic assessment. However, its clinical utility is limited by variability in immunohistochemical assessment, influenced by numerous factors, including differences in antibody clones. This study aims to compare the performance of two widely used Ki-67 antibody clones, MIB1 and SP6, and to evaluate their impact on molecular subtyping in breast cancer. Sections from formalin-fixed, paraffin-embedded biopsy samples from 35 primary breast carcinoma cases were stained using MIB1 and SP6 antibodies. Histological tumor characteristics and receptor statuses were evaluated for each case. The Ki-67 proliferative index was assessed following the latest recommendations of the International Ki-67 in Breast Cancer Working Group. Statistical analysis included Pearson correlation, paired <em>t</em>-test, Welch's ANOVA, and McNemar's test. A strong correlation was observed between Ki-67 indices obtained using MIB1 and SP6 (<em>r</em> = 0.755, <em>p</em> &lt; 0.001), with no statistically significant difference in mean values (<em>p</em> = 0.288). Ki-67 index correlated significantly with tumor grade for both antibodies. Among Luminal HER2-negative tumors, 17 % showed discordant molecular subtyping between MIB1 and SP6 using a 20 % cut-off. Similar discrepancies were observed at 14 % and 25 % cut-offs, suggesting that antibody choice may affect subtype classification. In conclusion, both MIB1 and SP6 provide reliable assessment of proliferative index in breast carcinoma, however, discrepancies in individual cases may impact molecular subtyping and subsequent clinical decision-making. Therefore, reporting the antibody clone used in Ki-67 evaluation may be advisable, pending further validation.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"79 ","pages":"Article 152523"},"PeriodicalIF":1.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between fibroblastic foci and respiratory function: Does the abundance of fibroblastic foci reflect a recent decline in respiratory function?","authors":"Hiroyuki Katsuragawa ,&nbsp;Hiroaki Ito ,&nbsp;Tomohiro Handa ,&nbsp;Masatsugu Hamaji ,&nbsp;Satona Tanaka ,&nbsp;Ryo Sakamoto ,&nbsp;Daisuke Nakajima ,&nbsp;Hiroshi Date ,&nbsp;Hironori Haga ,&nbsp;Akihiko Yoshizawa","doi":"10.1016/j.anndiagpath.2025.152522","DOIUrl":"10.1016/j.anndiagpath.2025.152522","url":null,"abstract":"<div><h3>Aims</h3><div>Fibroblastic foci (FF) are main findings in idiopathic pulmonary fibrosis (IPF) but are not specific to IPF. Pirfenidone and nintedanib are standard antifibrotic treatments for IPF and affect factors associated with fibroblasts. A proportion of interstitial lung diseases (ILDs) are progressive fibrosing ILDs (PF-ILDs). This progressive fibrosing phenotype includes various ILDs, including fibrotic hypersensitivity pneumonitis (FHP) and connective tissue disease-related ILD (CTD-ILD). We examined the relationship between FF and a relative decline in respiratory function.</div></div><div><h3>Methods and results</h3><div>Among patients with lung transplantation (LT), those diagnosed with IPF, nonspecific interstitial pneumonia (NSIP), CTD-ILD, and FHP for whom respiratory function test results within 24 months before LT were retrospectively available were included (<em>n</em> = 67). Patients were classified as PF-ILD+ or PF-ILD- based on the criteria for the progression of a relative decline in the predicted value of forced vital capacity (FVC) within 24 months before LT. We classified FF into peripheral (pFF), alveolar (aFF), centrilobular (cFF), and distorted or dense fibrotic lesions (dFF). The number of FF/cm² at each location was counted, and its percentage was calculated. Spearman's rank correlation coefficient between a relative decline in %FVC and total FF/cm² in NSIP was 0.721. The dFF/cm² and dFF/total FF ratios were higher and the aFF/total FF ratio was lower in the PF-ILD+ group than in the PF-ILD- group.</div></div><div><h3>Conclusion</h3><div>Total FF correlated with relative declines in %FVC in NSIP. Higher dFF/total FF ratios were associated with progressive status, and higher aFF/total FF ratios were associated with less progressive status.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"79 ","pages":"Article 152522"},"PeriodicalIF":1.5,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144366229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the frequency of meningothelial hyperplasia and its clinicopathological correlation in patients diagnosed with subdural hematoma","authors":"Fadime Eda Gökalp Satıcı ,&nbsp;Gözde Arslan ,&nbsp;Hamide Sayar","doi":"10.1016/j.anndiagpath.2025.152521","DOIUrl":"10.1016/j.anndiagpath.2025.152521","url":null,"abstract":"<div><div>Subdural hematomas develop as a result of hemorrhages in bridging veins located within the subdural space, between the dura mater and arachnoid mater. Histopathological examination of surgical specimens from hematoma regions may reveal meningothelial cell hyperplasia secondary to the hemorrhage. This study aims to evaluate the frequency of meningothelial hyperplasia (MH), cell count and size observed in these specimens and to highlight this proliferation which may mimic meningioma in differential diagnosis. Histopathological slides of 68 patients operated for subdural hematoma were retrospectively analyzed. Membrane thickness and the cell count and size of hyperplasia in areas with MH were measured. Associations with clinical parameters such as age, sex, hypertension, diabetes mellitus, and chronic kidney disease, as well as histopathological parameters like granulation tissue and psammoma bodies were investigated. MH was observed in 23 patients (33.8 %) and absent in 45 patients (66.2 %). The meningothelial cell layer count ranged from a minimum of 3 to a maximum of 15, with an average cell count of 9.6. The average size of the MH areas was 482 μm and the mean membrane thickness across all patients was 1.8 mm. Psammoma bodies were observed in 23 patients (33.8 %) and granulation tissue was seen in 25 patients (36.8 %). The presence of MH was found to be associated with increasing age (<em>p</em> = 0.021) but was unrelated to hypertension, diabetes, or chronic kidney disease. However, increased membrane thickness was observed in patients with hypertension (<em>p</em> = 0.029). In conclusion, this study investigated the frequency of MH which is a reactive process in subdural hematoma specimens and further clinicopathological studies are crucial for better understanding meningothelial cells, which are also the origin of meningiomas.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"79 ","pages":"Article 152521"},"PeriodicalIF":1.5,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of fine needle aspiration cytology in the diagnosis of mediastinal lesions: A 9-year experience from coastal Karnataka","authors":"Chaithra Gowthuvalli Venkataramana ,&nbsp;Shalini Radhakrishnan ,&nbsp;K.M. Sinchana ,&nbsp;Aditi Dixit ,&nbsp;Kocherlakota Krishna Akhil ,&nbsp;Rakshatha Nayak ,&nbsp;Sunita Hegde ,&nbsp;Shikha Jayasheelan","doi":"10.1016/j.anndiagpath.2025.152520","DOIUrl":"10.1016/j.anndiagpath.2025.152520","url":null,"abstract":"<div><div>Fine needle aspiration cytology has emerged as a minimally invasive, cost-effective diagnostic tool with significant utility in evaluating mediastinal lesions. However, comprehensive data on its diagnostic accuracy, efficacy, and clinical impact on managing these lesions remain limited. This study aims to evaluate the diagnostic utility of fine needle aspiration cytology in mediastinal lesions, focusing on its accuracy, safety, and role in guiding therapeutic approaches. A cross-sectional retrospective analysis was conducted on patients who underwent fine needle aspiration for mediastinal lesions at our tertiary care centre between 2015 and 2024. Demographic data, cytological findings, histopathological correlation, and ancillary studies were reviewed. Diagnostic accuracy was assessed by comparing fine needle aspiration cytology findings with histopathological diagnoses. A cumulative total of 60 cases of mediastinal lesions were evaluated, of which 47 cases were considered suitable for cytological assessment. Among the 60 cases, 41 cases were identified as neoplastic (68.33 %), while 6 were classified as nonneoplastic (10 %) on cytology. Within the spectrum of neoplastic mediastinal lesions, lymphoma was recognised as the principal neoplastic entity, followed by thymic neoplasms and metastatic lesions. The overall diagnostic accuracy achieved through fine needle aspiration cytology in distinguishing these lesions was established at 83.8 %, with diagnostic accuracy being exceptionally high in metastatic lesions. Fine needle aspiration cytology of mediastinal lesions is a highly accurate, safe, and valuable diagnostic tool that significantly influences clinical management and treatment protocols. However, further studies with larger sample sizes and prospective designs are warranted to validate these results and refine procedural protocols.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"79 ","pages":"Article 152520"},"PeriodicalIF":1.5,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactivity of carbonic anhydrase IX (CA IX) across the spectrum of renal cell carcinomas with sarcomatoid differentiation","authors":"Maryna Slisarenko ,&nbsp;Reza Alaghehbandan ,&nbsp;Joanna Rogala ,&nbsp;Mihaela Farcas ,&nbsp;Josef Skopal ,&nbsp;Marian Svajdler ,&nbsp;Ondrej Fiala ,&nbsp;Petr Stránský Jr. ,&nbsp;Adriena Bartos Vesela ,&nbsp;Tomas Pitra ,&nbsp;Milan Hora ,&nbsp;Michal Michal ,&nbsp;Ondrej Hes ,&nbsp;Kristyna Pivovarcikova","doi":"10.1016/j.anndiagpath.2025.152517","DOIUrl":"10.1016/j.anndiagpath.2025.152517","url":null,"abstract":"<div><div>Carbonic anhydrase IX (CA IX) is traditionally considered to be an immunomarker of clear cell renal cell carcinoma (RCC). However, CA IX expression has also been documented in other RCCs subtypes. Discrimination between clear cell RCC and non-clear cell RCC is crucial for further patient management. The aim of this study was to assess CA IX immunoreactivity across the spectrum of RCC with sarcomatoid differentiation. The expression of CA IX was evaluated in 32 cases of RCCs with sarcomatoid differentiation (12 clear cell RCC, 7 papillary RCC and 13 chromophobe RCC). Seven urothelial carcinomas (UC) with sarcomatoid differentiation (originally from renal pelvis) and 23 soft tissue tumors were also included as a control cohort. The sensitivity for CA IX in sarcomatoid component of clear cell RCC was 91.7 % (moderate/strong CA IX staining in &gt;60 % of sarcomatoid component). However, the CA IX specificity was rather low (45 %), as a significant proportion of sarcomatoid components in different renal cell carcinoma subtypes also stained with CA IX (2/7 papillary RCC, 9/14 chromophobe RCC). When urothelial carcinoma and soft tissue tumors were included in the evaluation, the specificity of CA IX staining for sarcomatoid clear cell RCC reached 60 %. In conclusion, CA IX shows decent sensitivity for sarcomatoid clear cell RCC, but with low specificity, hence limiting its diagnostic utility as a reliable marker of in tumors with predominant sarcomatoid component.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"79 ","pages":"Article 152517"},"PeriodicalIF":1.5,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Letter to the Editor: Nuclear pseudo inclusion is associated with BRAFV600E mutation: Analysis of nuclear features in papillary thyroid carcinoma”","authors":"Noor Ul Huda ,&nbsp;Hafsa Ahsun ,&nbsp;Muhammad Fahad Mukhtar","doi":"10.1016/j.anndiagpath.2025.152516","DOIUrl":"10.1016/j.anndiagpath.2025.152516","url":null,"abstract":"<div><div>Harahap et al. (2025) examine the association between BRAFV600E mutation and nuclear features in papillary thyroid carcinoma (PTC), with a focus on nuclear pseudo inclusions (NPIs). While the study contributes to understanding molecular-histo pathological correlations, several critical limitations warrant attention. The authors do not account for congenital anomalies such as thyroid hemi agenesis, which can alter tumor morphology independently of genetic mutations [2]. Additionally, coexisting autoimmune thyroid conditions like Hashimoto’s thyroiditis may confound nuclear features through inflammatory changes [3]. The study also overlooks the influence of demographic factors, such as age and gender, which are known to impact PTC prognosis and histology [4]. Reliance on a subjective nuclear scoring system without digital validation raises concerns about reproducibility and inter observer variability [5]. Addressing these gaps is significant to improve the diagnostic accuracy and clinical relevance of morpho-molecular correlations in Papillary Thyroid Carcinoma. Future research should adopt more comprehensive and standardized approaches.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"79 ","pages":"Article 152516"},"PeriodicalIF":1.5,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small but significant: Prognostic value of the small duct type in intrahepatic cholangiocarcinoma","authors":"Jiyun Kim,&nbsp;Joon Young Park,&nbsp;So Young Kim,&nbsp;Sejin Jung,&nbsp;Joo-Young Na,&nbsp;Hyun Jung Lee,&nbsp;Dong Hoon Shin,&nbsp;Jung Hee Lee","doi":"10.1016/j.anndiagpath.2025.152515","DOIUrl":"10.1016/j.anndiagpath.2025.152515","url":null,"abstract":"<div><div>This study aimed to evaluate the clinicopathological features, prognostic relevance, and classification accuracy of histological subtypes—the small duct type and the large duct type—in intrahepatic cholangiocarcinoma (iCCA) based on the WHO 5th edition criteria. A retrospective analysis was conducted on iCCA cases classified into the small duct type and the large duct type. Key pathological variables were identified using logistic regression and used to develop a random forest classification model. Survival outcomes were analyzed using Kaplan-Meier and Cox regression analyses. The random forest model, using nine significant pathological features, achieved an overall classification accuracy of 83 %. Mucin production, tumor grade, and intraepithelial lesion were among the most influential predictors. The small duct type was associated with significantly better overall survival (5-year rate: 57.6 %) and recurrence-free survival (46.6 %) compared to the large duct type (24.9 % and 20.6 %, respectively). Multivariable analysis confirmed the large duct type as an independent predictor of worse prognosis. Histological subtyping of iCCA holds important prognostic value. The small duct type is associated with a more favorable clinical course, supporting the integration of subtype-aware assessments into diagnostic and prognostic frameworks. These findings may inform future developments in computational pathology and personalized treatment strategies.</div></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"79 ","pages":"Article 152515"},"PeriodicalIF":1.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Comment on Mucinous Neoplasms Associated with Mature Cystic Teratomas: A Clinicopathologic Study of 50 Cases: Are We Ready for Nomenclature Change?","authors":"Vinita Parkash","doi":"10.1016/j.anndiagpath.2025.152514","DOIUrl":"10.1016/j.anndiagpath.2025.152514","url":null,"abstract":"","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"78 ","pages":"Article 152514"},"PeriodicalIF":1.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosai-Dorfman disease of the breast: A six-case clinicopathologic study","authors":"Elif Demirci ,&nbsp;Mostafa Kokabee ,&nbsp;Aruuke Sulaimanova ,&nbsp;Diana Kantarovich ,&nbsp;Iskender Genco ,&nbsp;Shabnam Jaffer ,&nbsp;Alyssa Yurovitsky ,&nbsp;Sabina Hajiyeva","doi":"10.1016/j.anndiagpath.2025.152513","DOIUrl":"10.1016/j.anndiagpath.2025.152513","url":null,"abstract":"","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"79 ","pages":"Article 152513"},"PeriodicalIF":1.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OBITUARY: RENE J. BUESA, Ph.D., HTL (ASCP)","authors":"Saul Suster","doi":"10.1016/j.anndiagpath.2025.152503","DOIUrl":"10.1016/j.anndiagpath.2025.152503","url":null,"abstract":"","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"79 ","pages":"Article 152503"},"PeriodicalIF":1.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}