Annals of Diagnostic Pathology最新文献_第10页

Is mediastinal fine needle aspiration cytology required or redundant? A single institution-based correlation study with core needle biopsy 纵隔细针穿刺细胞学检查是必要还是多余？基于单一机构的核心针活检相关性研究

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-29 DOI: 10.1016/j.anndiagpath.2024.152300

Pavneet Kaur Selhi , Rashi Aggarwal , Sumit Grover , Simmi Klar , Ruchita Tyagi , Arshneet Kaur Selhi , Siddharth Prakash

{"title":"Is mediastinal fine needle aspiration cytology required or redundant? A single institution-based correlation study with core needle biopsy","authors":"Pavneet Kaur Selhi , Rashi Aggarwal , Sumit Grover , Simmi Klar , Ruchita Tyagi , Arshneet Kaur Selhi , Siddharth Prakash","doi":"10.1016/j.anndiagpath.2024.152300","DOIUrl":"https://doi.org/10.1016/j.anndiagpath.2024.152300","url":null,"abstract":"<div><h3>Introduction</h3><p>Fine Needle Aspiration cytology (FNAC) and core needle biopsy (CNB) are rapid, minimally invasive and useful techniques to evaluate mediastinal lesions.</p></div><div><h3>Objectives</h3><p>To compare cytopathology with histopathology of mediastinal lesions and analyse reasons for discordance.</p></div><div><h3>Material & methods</h3><p>Retrospective analysis was done in a tertiary care centre in North West India over a period of seven and half years from 1<sup>st</sup>July 2016 to 31st December 2023. Only those patients who had undergone FNAC and trucut biopsy of mediastinal masses were included. The cytopathology and histopathology slides were studied to analyse causes of discordance. Sensitivity, specificity, positive and negative predictive values of FNAC were calculated keeping histopathology as gold standard.</p></div><div><h3>Results</h3><p>Out of 57 cases analysed, eight cases were non diagnostic on cytology. Cytology could effectively classify a lesion as non neoplastic (7) or neoplastic (42). For further subtyping, histopathology and Immunohistochemistry (IHC) were required. Out of 27 cases of cytological – histopathological discordance, 8 cases had sampling error, 15 cases had limited concordance where FNAC could predict possibility of tumor and 4 cases were discordant where subtyping of malignancy varied on CNB. Sensitivity of FNAC to predict definite diagnosis was 90.2 %, specificity was 50 %, positive predictive value of FNAC to give a definite diagnosis was 93.9 %, negative predictive value was 37.5 %.</p></div><div><h3>Conclusion</h3><p>Evaluation of mediastinal masses requires combination of cytology, histopathology and ancillary techniques like IHC. FNAC and CNB are complementary modalities and both are essential for rapid, accurate and comprehensive diagnosis.</p></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"71 ","pages":"Article 152300"},"PeriodicalIF":2.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140341841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ependymomas of the spinal region in adults: Clinical and pathological features and MYCN expression levels in spinal ependymomas and myxopapillary ependymomas 成人脊柱区域的附肢瘤：脊髓上皮瘤和肌乳头状上皮瘤的临床和病理特征及 MYCN 表达水平

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-27 DOI: 10.1016/j.anndiagpath.2024.152299

Fikret Dirilenoğlu , Gözde Topel , İsmail Ertan Sevin , Aslı Kahraman

{"title":"Ependymomas of the spinal region in adults: Clinical and pathological features and MYCN expression levels in spinal ependymomas and myxopapillary ependymomas","authors":"Fikret Dirilenoğlu , Gözde Topel , İsmail Ertan Sevin , Aslı Kahraman","doi":"10.1016/j.anndiagpath.2024.152299","DOIUrl":"https://doi.org/10.1016/j.anndiagpath.2024.152299","url":null,"abstract":"<div><h3>Background</h3><p>Ependymomas (EPNs) of the spinal region are a heterogeneous group of tumors that account for 17.6 % in adults. Four types have been recognized: subependymoma, spinal ependymoma (Sp-EPN), myxopapillary ependymoma (MPE), and Sp-EPN-MYCN amplified, each with distinct histopathological and molecular features.</p></div><div><h3>Methods</h3><p>This study investigated the clinical and pathological characteristics and MYCN expression levels of 35 Sp-EPN and MPE cases diagnosed at a tertiary university hospital over a decade-long period.</p></div><div><h3>Results</h3><p>Twenty-five cases were Sp-EPN and 10 cases were MPE, and were graded as WHO grade 2, except for 1 Sp-EPN case with grade 3 features. The most common symptoms were lower back pain and difficulty in walking. Radiology showed different tumor sizes and locations along the spinal cord, with MPEs exclusively in the lumbosacral region. Surgery was the main treatment, and gross total resection was achieved in all cases except for one. Immunohistochemistry showed low Ki-67 proliferation indices in all cases, and no MYCN expression. During follow-up, 3 (8.6 %) cases recurred and/or metastasized and 5 cases (14.3 %) died. No significant difference was found in disease-free survival or overall survival between Sp-EPN and MPE cases. However, 3 cases with grade 2 histology demonstrated recurrence and/or metastasis, despite the lack of MYCN expression.</p></div><div><h3>Conclusion</h3><p>Our results underscore the multifactorial nature of tumor aggressiveness in EPNs of the spinal region. This study enhances our knowledge of the clinical and pathological features of Sp-EPNs and MPEs and highlights the need for better diagnostic and prognostic markers in these rare tumors.</p></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"70 ","pages":"Article 152299"},"PeriodicalIF":2.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140328632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential diagnosis of gastric low- and high grade dysplasia using C6orf15 protein 利用 C6orf15 蛋白对胃低度和高度发育不良进行鉴别诊断

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-26 DOI: 10.1016/j.anndiagpath.2024.152298

Leilei Liu , Xuan Wang , Qibin He , Bo Yu , Jiandong Wang , Hong Shen

{"title":"Differential diagnosis of gastric low- and high grade dysplasia using C6orf15 protein","authors":"Leilei Liu , Xuan Wang , Qibin He , Bo Yu , Jiandong Wang , Hong Shen","doi":"10.1016/j.anndiagpath.2024.152298","DOIUrl":"https://doi.org/10.1016/j.anndiagpath.2024.152298","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the expression of C6orf15 protein in gastric endoscopic biopsy specimens and its usage as an ancillary diagnostic biomarker in determining the grade of gastric dysplasia.</p></div><div><h3>Methods</h3><p>We selected 102 patients with gastric endoscopic biopsy specimens from Jinling Hospital. These were divided into four groups: 22 cases of gastric mucosal benign lesions, 28 with low-grade dysplasia (LGD, intestinal-type: 21 cases，foveolar-type: 7cases), 28 with high-grade dysplasia (HGD, intestinal-type: 20 cases，foveolar-type: 8 cases), and 24 cases of gastric adenocarcinoma. We examined the expressions of C6orf15, P53, and Ki67 in 102 gastric endoscopic biopsy specimens, including 47 cases with accompanying endoscopic submucosal dissection (ESD) specimens, using immunohistochemistry.</p></div><div><h3>Results</h3><p>In gastric HGD and gastric adenocarcinoma, the c6orf15 protein exhibits diffuse and strong cytoplasmic expression in tumor cells. Conversely, in gastric LGD and benign gastric mucosal lesions, the c6orf15 protein shows negative or faint yellow cytoplasmic staining. The expression rate of C6orf15 in high-grade gastric dysplasia (HGD, 93 %) and gastric adenocarcinoma (100 %) was significantly higher than in the gastric mucosal benign lesion group (0 %) and the low-grade dysplasia (LGD, 7 %) group (<em>P</em> < 0.001).</p></div><div><h3>Conclusion</h3><p>The detection of C6orf15 protein expression could serve as a valuable adjunctive diagnostic tool for distinguishing between gastric HGD, LGD, and benign lesions. The combined assessment of C6orf15, P53, and Ki67 expressions may be beneficial in determining the grade of gastric dysplasia and evaluating the risk of progression in gastric mucosal lesions in clinical practice.</p></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"71 ","pages":"Article 152298"},"PeriodicalIF":2.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alpha-methyl CoA racemase (AMACR) reactivity across the spectrum of clear cell renal cell neoplasms 透明细胞肾细胞瘤谱中α-甲基辅酶A（AMACR）的反应性

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-24 DOI: 10.1016/j.anndiagpath.2024.152297

Pavla Rotterova , Reza Alaghehbandan , Josef Skopal , Joanna Rogala , Maryna Slisarenko , Andrea Strakova Peterikova , Kvetoslava Michalova , Delia Perez Montiel , Mihaela Farcas , Monika Ulamec , Petr Stransky Jr , Ondrej Fiala , Tomas Pitra , Milan Hora , Michal Michal , Kristyna Pivovarcikova , Ondrej Hes

{"title":"Alpha-methyl CoA racemase (AMACR) reactivity across the spectrum of clear cell renal cell neoplasms","authors":"Pavla Rotterova , Reza Alaghehbandan , Josef Skopal , Joanna Rogala , Maryna Slisarenko , Andrea Strakova Peterikova , Kvetoslava Michalova , Delia Perez Montiel , Mihaela Farcas , Monika Ulamec , Petr Stransky Jr , Ondrej Fiala , Tomas Pitra , Milan Hora , Michal Michal , Kristyna Pivovarcikova , Ondrej Hes","doi":"10.1016/j.anndiagpath.2024.152297","DOIUrl":"https://doi.org/10.1016/j.anndiagpath.2024.152297","url":null,"abstract":"<div><p>a-Methylacyl coenzyme A racemase (AMACR) is traditionally considered to be a marker of papillary renal cell carcinoma. However, AMACR expression can be seen in other renal tumors. The aim of this study was to investigate AMACR immunoreactivity within the spectrum of clear cell renal cell neoplasms.</p><p>Fifty-three clear cell renal epithelial tumors were used in assembling the following four cohorts: low grade (LG) clear cell renal cell carcinoma (CCRCC), high grade (HG) CCRCC, CCRCC with cystic changes, and multilocular cystic renal neoplasm of low malignant potential (MCRNLMP). Representative blocks were stained for AMACR, using two different clones (SP52 and OV-TL12/30).</p><p>There were at least some AMACR immunoreactivity in 77.8 % and 68.9 % of CCRCCs (using SP52 and OV-TL12/30 clone, respectively). Moderate to strong positivity, or positivity in more than one third of the tumor (even weak in intensity) was detected in 46.7 % of CCRCCs using SP52 and in 48.9 % of CCRCC using OV-TL12/30 clone. The highest AMACR reactivity was observed in HG CCRCC (60 % by SP52 and 66.7 % by OV-TL12/30). Strong and diffuse AMACR positivity was detected in 8.9 % of all CCRCCs. AMACR immunoreactivity in MCRNLMP was 37.5 % (SP52 clone) and 25 % (OV-TL12/30 clone).</p><p>We demonstrated relatively high expression rate of AMACR in CCRCC, while very variable in intensity and distribution. This finding may have diagnostic implications especially in limited samples (i.e., core biopsies), as AMACR positivity does not exclude the diagnosis of CCRCC.</p></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"71 ","pages":"Article 152297"},"PeriodicalIF":2.0,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140344304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital image analysis of Ki67 hotspot detection and index counting in gastroenteropancreatic neuroendocrine neoplasms 对 Ki67 热点检测和指数计数胃肠胰神经内分泌肿瘤的数字图像分析

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-20 DOI: 10.1016/j.anndiagpath.2024.152295

Kritsanu Saetiew , Napat Angkathunyakul , Saowalak Hunnangkul , Ananya Pongpaibul

{"title":"Digital image analysis of Ki67 hotspot detection and index counting in gastroenteropancreatic neuroendocrine neoplasms","authors":"Kritsanu Saetiew , Napat Angkathunyakul , Saowalak Hunnangkul , Ananya Pongpaibul","doi":"10.1016/j.anndiagpath.2024.152295","DOIUrl":"10.1016/j.anndiagpath.2024.152295","url":null,"abstract":"<div><p>The Ki-67 proliferative index plays a pivotal role in the subclassification of neuroendocrine neoplasm (NEN) according to the WHO Classification of Digestive System Tumors (5th edition), which designates neuroendocrine tumor (NET) grades 1, 2, and 3 for Ki-67 proliferative index of <3 %, 3–20 %, and >20 %, respectively. Proliferative index calculation must be performed in the hotspot, traditionally selected by visual scanning at low-power magnification. Recently, gradient map visualization has emerged as a tool for various purposes, including hotspot selection. This study includes 97 cases of gastrointestinal neuroendocrine neoplasms, with hotspots selected by bare eye and gradient map visualization (GM). Each hotspot was analyzed using three methods: eye estimation (EE), digital image analysis (DIA), and manual counting.</p><p>Of the NENs studied, 91 % were NETs (26 % for G1, 55 % for G2, and 10 % for G3). Only 9 cases were neuroendocrine carcinoma (NEC). Between two hotspot selection methods, GM resulted in a higher grade in 14.77 % of cases, primarily upgrading from NET G1 to G2. Among the counting methods, DIA demonstrated substantial agreement with manual counting, both for pathologist and resident. Grading by other methods tended to result in a higher grade than MC (26.99 % with EE and 8.52 % with DIA).</p><p>Given its clinical and statistical significance, this study advocates for the application of GM in hotspot selection to identify higher-grade tumors. Furthermore, DIA provides accurate grading, offering time efficiency over MC.</p></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"71 ","pages":"Article 152295"},"PeriodicalIF":2.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140274802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the archives of MD Anderson Cancer Center. Mesothelial/monocytic incidental cardiac excrescence with a review of the literature 来自 MD 安德森癌症中心的档案。间皮细胞/单核细胞偶发性心脏赘生物及文献综述

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-20 DOI: 10.1016/j.anndiagpath.2024.152296

Jayati Mallick, Beenu Thakral, Qing Wei, L. Jeffrey Medeiros

{"title":"From the archives of MD Anderson Cancer Center. Mesothelial/monocytic incidental cardiac excrescence with a review of the literature","authors":"Jayati Mallick, Beenu Thakral, Qing Wei, L. Jeffrey Medeiros","doi":"10.1016/j.anndiagpath.2024.152296","DOIUrl":"https://doi.org/10.1016/j.anndiagpath.2024.152296","url":null,"abstract":"<div><p>Mesothelial/monocytic incidental cardiac excrescence (MICE) is a rare benign lesion composed of monocytes and mesothelial cells that is most often encountered during cardiothoracic surgery. We describe a case in a 71-year-old man with known aortic valve stenosis who presented with gradual onset dyspnea over a few weeks, made worse with minimal exertion. A transesophageal echocardiogram revealed severe aortic stenosis and mild pericardial effusion. The patient underwent aortic valve replacement, coronary artery bypass, and amputation of the left atrial appendage. Histological examination of a 0.8 cm blood clot received along with the atrial appendage showed an aggregation of bland cells with features of monocytes associated with small strands and nodules of mesothelial cells, fat cells, fibrin and a minute fragment of bone. Immunohistochemical analysis showed that the monocytic cells were positive for CD4 and CD68 (strong) and negative for calretinin and keratin. By contrast, the mesothelial cells were positive for calretinin and keratin and negative for all other markers. In sum, the morphologic and immunohistochemical findings support the diagnosis of MICE. Based on our review of the literature, about 60 cases of MICE have been reported previously which we have tabulated. We also discuss the differential diagnosis.</p></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"71 ","pages":"Article 152296"},"PeriodicalIF":2.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140209536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the relationship between tumor-infiltrating lymphocytes and PD-L1 expression in triple negative breast cancer: Identifying optimal TILs cut-off value for pathologic reporting 评估三阴性乳腺癌中肿瘤浸润淋巴细胞与 PD-L1 表达之间的关系：为病理报告确定最佳TILs临界值

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-16 DOI: 10.1016/j.anndiagpath.2024.152294

Krit Suwannaphoom, Sithapong Soontornsit, Kulachet Wiwatwarayos, Prapapun Seneetuntigul, Parsinee Julimasart

{"title":"Assessing the relationship between tumor-infiltrating lymphocytes and PD-L1 expression in triple negative breast cancer: Identifying optimal TILs cut-off value for pathologic reporting","authors":"Krit Suwannaphoom, Sithapong Soontornsit, Kulachet Wiwatwarayos, Prapapun Seneetuntigul, Parsinee Julimasart","doi":"10.1016/j.anndiagpath.2024.152294","DOIUrl":"https://doi.org/10.1016/j.anndiagpath.2024.152294","url":null,"abstract":"<div><h3>Background</h3><p>Triple Negative Breast Cancer (TNBC) presents diagnostic complexities, particularly in evaluating Tumor-Infiltrating Lymphocytes (TILs) and Programmed Death-Ligand 1 (PD-L1) expression. This study aimed to identify optimal TILs percentage cut-offs predictive of PD-L1 expression and to investigate the relationship between TILs, PD-L1, and tertiary lymphoid structures (TLSs).</p></div><div><h3>Method</h3><p>Method: Analyzing 141 TNBC cases, we assessed TILs, PD-L1 expression (clones 22C3 and SP142), and TLS presence.</p></div><div><h3>Results</h3><p>We identified TILs cut-offs (<20 %, 20–60 %, ≥60 %) correlating with PD-L1 expression. TILs <20 % rarely express PD-L1 with either 22C3 or SP142 clones. TILs ≥60 % demonstrate PD-L1 expression across both clones. TILs within the 20–60 % range correlate with PD-L1 expression using the SP142 clone, but not 22C3. Evaluating TILs solely at the tumor edge led to inaccuracies, highlighting the need for overall assessment of TILs throughout the entire lesion. TLS presence correlated with higher TIL percentages and PD-L1 expression, particularly with SP142. Discrepancies between 22C3 and SP142 clones (15 % vs. 50 % positivity, respectively) underscored the variability in PD-L1 detection.</p></div><div><h3>Conclusion</h3><p>This study identifies TILs cut-offs predictive of PD-L1 positivity, suggesting the need for institutions to tailor these thresholds based on the selected PD-L1 clone and treatment. Evaluating TILs solely at the tumor edge may overlook the complexity of tumor immune infiltration. While TLS presence correlates with higher PD-L1 expression, particularly with the SP142 clone, its exact predictive value for PD-L1 remains to be clarified. The SP142 clone exhibits higher positivity rates compared to 22C3.</p></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"70 ","pages":"Article 152294"},"PeriodicalIF":2.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140162409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the archives of MD Anderson Cancer Center: Monomorphic epitheliotropic intestinal T-cell lymphoma: A case with an unusual immunophenotype and discussion of differential diagnosis 来自 MD 安德森癌症中心的档案：单形上皮细胞性肠T细胞淋巴瘤：一例异常免疫表型病例及鉴别诊断讨论

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-08 DOI: 10.1016/j.anndiagpath.2024.152293

Nicholas J. Dcunha, Qing Wei, Beenu Thakral, L. Jeffrey Medeiros

{"title":"From the archives of MD Anderson Cancer Center: Monomorphic epitheliotropic intestinal T-cell lymphoma: A case with an unusual immunophenotype and discussion of differential diagnosis","authors":"Nicholas J. Dcunha, Qing Wei, Beenu Thakral, L. Jeffrey Medeiros","doi":"10.1016/j.anndiagpath.2024.152293","DOIUrl":"https://doi.org/10.1016/j.anndiagpath.2024.152293","url":null,"abstract":"<div><p>Monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL) is a rare and aggressive T-cell neoplasm associated with poor survival. We report a case of MEITL that presented as an ulcerated mass in the jejunum with perforation. Microscopic examination showed that the neoplasm involved the full thickness of the intestinal wall, extended into the mesentery, and was composed of monomorphic, small to medium-size cells. Immunohistochemical analysis showed that the neoplastic cells were positive for T-cell receptor (TCR) delta, CD3, CD7, CD8 (small subset), BCL-2 and TIA-1, and negative for TCR beta, CD4, CD5, CD10, CD20, CD30, CD34, CD56, CD57, CD99, ALK, cyclin D1, granzyme B, MUM1/IRF4, and TdT. The Ki-67 proliferation index was approximately 50 %. In situ hybridization for Epstein-Barr virus-encoded RNA (EBER ISH) was negative. Next-generation sequencing (NGS) analysis showed mutations involving <em>SETD2</em> and <em>STAT5B</em>. The patient was treated with aggressive chemotherapy and consolidative autologous stem cell transplant and had clinical remission, but relapsed after about one year. Retreatment led to another one-year interval of clinical remission, but at last follow up the patient has relapsed disease involving the ileum and colon. We also discuss the differential diagnosis of MEITL.</p></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"70 ","pages":"Article 152293"},"PeriodicalIF":2.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140123221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interpreting HER2-low at the lower end: Added challenge to the pathologists 在低端解读 HER2-低值：病理学家面临的新挑战

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-07 DOI: 10.1016/j.anndiagpath.2024.152291

Thaer Khoury

引用次数: 0

Analysis of glomerular deposition of IgM and C3 in patients with podocytopathies 分析荚膜细胞病患者肾小球中 IgM 和 C3 的沉积情况

IF 2 4区医学

Annals of Diagnostic Pathology Pub Date : 2024-03-07 DOI: 10.1016/j.anndiagpath.2024.152292

Verônica Aparecida Ferreira , Crislaine Aparecida da Silva , José Rodrigues do Carmo Neto , Laura Penna Rocha , Ana Luisa Monteiro dos Santos Martins , Marlene Antônia dos Reis , Juliana Reis Machado

{"title":"Analysis of glomerular deposition of IgM and C3 in patients with podocytopathies","authors":"Verônica Aparecida Ferreira , Crislaine Aparecida da Silva , José Rodrigues do Carmo Neto , Laura Penna Rocha , Ana Luisa Monteiro dos Santos Martins , Marlene Antônia dos Reis , Juliana Reis Machado","doi":"10.1016/j.anndiagpath.2024.152292","DOIUrl":"https://doi.org/10.1016/j.anndiagpath.2024.152292","url":null,"abstract":"<div><p>Minimal Change Disease (MCD) and Focal Segmental Glomerulosclerosis (FSGS) are the main causes of nephrotic syndrome in the world. The complement system appears to play an important role in the pathogenesis of these diseases. To evaluate the deposition of immunoglobulins and particles of the complement system in renal biopsies of patients with FSGS and MCD and relate to laboratory data, we selected 59 renal biopsies from patients with podocytopathies, 31 from patients with FSGS and 28 with MCD. Epidemiological, clinical, laboratory information and the prognosis of these patients were evaluated. Analysis of the deposition of IgM, IgG, C3, C1q and C4d in renal biopsies was performed. We related IgM and C3 deposition with laboratory parameters. Statistical analysis was performed using GraphPad Prism version 7.0. Glomerular deposition of IgM was significantly higher in the FSGS group, as was codeposition of IgM and C3. The clinical course of patients and laboratory data were also worse in cases of FSGS, with a higher percentage progressing to chronic kidney disease and death. Patients with C3 deposition had significantly higher mean serum creatinine and significantly lower eGFR, regardless of disease. Patients with FSGS had more IgM and C3 deposition in renal biopsies, worse laboratory data and prognosis than patients with MCD. C3 deposition, both in FSGS and MCD, appears to be related to worsening renal function.</p></div>","PeriodicalId":50768,"journal":{"name":"Annals of Diagnostic Pathology","volume":"70 ","pages":"Article 152292"},"PeriodicalIF":2.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140123220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0