Comparative Cytogenetics最新文献_第7页

Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses. 基于mtDNA和核型分析的中华绒螯蛙（Actinopterygii，Cottadae）的分化。

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-06-14 eCollection Date: 2021-01-01 DOI: 10.3897/CompCytogen.v15.i2.63207

Irina N Moreva, Olga A Radchenko, Anna V Petrovskaya

{"title":"Differentiation of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses.","authors":"Irina N Moreva, Olga A Radchenko, Anna V Petrovskaya","doi":"10.3897/CompCytogen.v15.i2.63207","DOIUrl":"https://doi.org/10.3897/CompCytogen.v15.i2.63207","url":null,"abstract":"A molecular genetic and karyological study of the frog sculpin Myoxocephalus stelleri Tilesius, 1811 was carried out on an extensive sample from a large area of the species' range. A total of 42 specimens was sampled from the Sea of Japan, Sea of Okhotsk, and coastal waters off the southern Kuril Islands, which makes this sampling scheme the most comprehensive to date. The level of mtDNA polymorphism was found to be low. The haplotypes of the species formed three phylogenetic groups. The unique M. stelleri haplotype from the coast of Shikotan Island linked all the studied groups, indicating that it is likely ancestral. Robertsonian polymorphism was identified in the species. In all five cytotypes (I - 2n = 44, II - 2n = 43, III - 2n = 42, IV - 2n = 41, V - 2n = 40; NF = 44+2) were identified, all of which were present in the Sea of Japan. Only one (cytotype I) was found in the Sea of Okhotsk, which is probably the closest to the ancestral karyotype. The significant chromosomal polymorphism and the presence of common haplotypes in the studied samples indicate their recent origin from a common ancestor and/or relatively recent contacts within the range. The discrepancies between mtDNA and karyotypes in assigning the ancestral M. stelleri to the coastal waters off Shikotan Island (southern Kuril Islands) and the Sea of Okhotsk, respectively, can be explained by the different inheritance mechanisms and the rates of evolution of molecular genetic and karyological traits.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"15 2","pages":"179-197"},"PeriodicalIF":1.0,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39101359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Unusual chromosomal polymorphism of the common shrew, Sorex araneus L., in southern Belarus. 白俄罗斯南部常见鼩鼱的异常染色体多态性。

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-06-04 eCollection Date: 2021-01-01 DOI: 10.3897/CompCytogen.v15.i2.63084

Iryna A Kryshchuk, Victor N Orlov, Elena V Cherepanova, Yury M Borisov

引用次数: 1

Chromosomes of three gall wasps of the tribe Aylacini (Hymenoptera, Cynipidae). 膜翅目，瘿蜂科三种瘿蜂的染色体。

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-06-04 eCollection Date: 2021-01-01 DOI: 10.3897/compcytogen.v15.i2.66781

Vladimir E Gokhman

{"title":"Chromosomes of three gall wasps of the tribe Aylacini (Hymenoptera, Cynipidae).","authors":"Vladimir E Gokhman","doi":"10.3897/compcytogen.v15.i2.66781","DOIUrl":"https://doi.org/10.3897/compcytogen.v15.i2.66781","url":null,"abstract":"Chromosomes of two species of the tribe Aylacini (Cynipidae), Isocolus jaceae (Schenck, 1863) and I. scabiosae (Giraud, 1859) (both have 2n = 18) were studied for the first time. In addition, 2n = 20 is confirmed in a member of the same tribe, Aulacidea hieracii (Bouché, 1834). All chromosomes of these gall wasps are biarmed; however, they gradually decrease in size in the case of A. hieracii, whereas a pair of large metacentrics is characteristic of karyotypes of both Isocolus Förster, 1869 species. Chromosomes of the two latter gall wasps are either metacentric or submetacentric, but elements with lower centromeric indices prevail in the karyotype of A. hieracii. Chromomycin A3 (CMA3)/DAPI staining revealed single CMA3-positive bands on a particular pair of chromosomes of all species, and these bands apparently refer to the nucleolus organizing regions (NORs). However, localization of CMA3-positive bands differs substantially between the studied members of Isocolus and Aulacidea Ashmead, 1897. Together with normal haploid and diploid mitotic divisions, several metaphase plates with 2n = 17 containing a peculiar dicentric chromosome were found in a single male specimen of I. scabiosae; this appears to be the first report of an obvious dicentric in the order Hymenoptera in general. Certain aspects of the chromosome diversity and karyotype evolution within the family Cynipidae and the tribe Aylacini in particular are briefly discussed.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"15 2","pages":"171-178"},"PeriodicalIF":1.0,"publicationDate":"2021-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39236212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Karyotype and COI sequences of Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera,Chironomidae) from the bay of Orkhon River, Mongolia Kiknadze et Siirin, 1999，内蒙古鄂尔孔河湾蠓科(双翅目，蠓科)的核型和COI序列

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-05-27 DOI: 10.3897/compcytogen.v15.i2.66549

Viktor V. Bolshakov,Alexander A. Prokin

{"title":"Karyotype and COI sequences of Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera,Chironomidae) from the bay of Orkhon River, Mongolia","authors":"Viktor V. Bolshakov,Alexander A. Prokin","doi":"10.3897/compcytogen.v15.i2.66549","DOIUrl":"https://doi.org/10.3897/compcytogen.v15.i2.66549","url":null,"abstract":"Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera, Chironomidae) is recorded from Mongolia for the first time. Eleven banding sequences determined in the Mongolian population were previously known from Altai and Yenisei populations: sokA1, sokB1, sokB2, sokC1, sokC2, sokD1, sokD2, sokE1, sokF1, sokF2 and sokG1. The additional B-chromosomes are absent. DNA-barcoding of COI gene was carried out for this species for the first time. The phylogenetic tree estimated by Bayesian inference showed a high similarity of the studied species with Ch. acutiventris Wülker, Ryser et Scholl, 1983 from the Chironomus obtusidens-group. The estimated genetic distance K2P between Ch. sokolovae and Ch. acutiventris is much lower (0.38%) than the commonly accepted threshold of 3% for species of genus Chironomus Meigen, 1803. Our results show that the accepted cytogenetic criteria of species level in the genus Chironomus are more in accordance with morphological ones of the same level, than with molecular-genetic criteria accepted for species COI genetic distance.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"59 1","pages":"149-157"},"PeriodicalIF":1.0,"publicationDate":"2021-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138528470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Karyotype and COI sequences of Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera,Chironomidae) from the bay of Orkhon River, Mongolia. Kiknadze et Siirin, 1999，内蒙古鄂尔孔河湾蠓科(双翅目，蠓科)的核型和COI序列。

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-05-27 eCollection Date: 2021-01-01 DOI: 10.3897/CompCytogen.v15i2.66549

Viktor V Bolshakov, Alexander A Prokin

{"title":"Karyotype and COI sequences of Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera,Chironomidae) from the bay of Orkhon River, Mongolia.","authors":"Viktor V Bolshakov, Alexander A Prokin","doi":"10.3897/CompCytogen.v15i2.66549","DOIUrl":"https://doi.org/10.3897/CompCytogen.v15i2.66549","url":null,"abstract":"Chironomus sokolovae Istomina, Kiknadze et Siirin, 1999 (Diptera, Chironomidae) is recorded from Mongolia for the first time. Eleven banding sequences determined in the Mongolian population were previously known from Altai and Yenisei populations: sokA1, sokB1, sokB2, sokC1, sokC2, sokD1, sokD2, sokE1, sokF1, sokF2 and sokG1. The additional B-chromosomes are absent. DNA-barcoding of COI gene was carried out for this species for the first time. The phylogenetic tree estimated by Bayesian inference showed a high similarity of the studied species with Ch. acutiventris Wülker, Ryser et Scholl, 1983 from the Chironomus obtusidens-group. The estimated genetic distance K2P between Ch. sokolovae and Ch. acutiventris is much lower (0.38%) than the commonly accepted threshold of 3% for species of genus Chironomus Meigen, 1803. Our results show that the accepted cytogenetic criteria of species level in the genus Chironomus are more in accordance with morphological ones of the same level, than with molecular-genetic criteria accepted for species COI genetic distance.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"15 2","pages":"149-157"},"PeriodicalIF":1.0,"publicationDate":"2021-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39065659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Genome size variation and karyotype diversity in eight taxa of Sorbus sensu stricto (Rosaceae) from China. 中国蔷薇科8个Sorbus stru stricto分类群基因组大小变异及核型多样性。

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-05-20 eCollection Date: 2021-01-01 DOI: 10.3897/CompCytogen.v15i2.58278

Jiabao Li, Kailin Zhu, Qin Wang, Xin Chen

{"title":"Genome size variation and karyotype diversity in eight taxa of Sorbus sensu stricto (Rosaceae) from China.","authors":"Jiabao Li, Kailin Zhu, Qin Wang, Xin Chen","doi":"10.3897/CompCytogen.v15i2.58278","DOIUrl":"https://doi.org/10.3897/CompCytogen.v15i2.58278","url":null,"abstract":"Eight taxa of Sorbus Linnaeus, 1753 sensu stricto (Rosaceae) from China have been studied karyologically through chromosome counting, chromosomal measurement and karyotype symmetry. Genome size was also estimated by flow cytometry. Six taxa, S. amabilis Cheng ex T.T.Yu et K.C.Kuan, 1963, S. hupehensis var. paucijuga (D.K. Zang et P.C. Huang, 1992) L.T. Lu, 2000, S. koehneana C.K. Schneider, 1906, S. pohuashanensis (Hance, 1875) Hedlund, 1901, S. scalaris Koehne, 1913 and S. wilsoniana C.K. Schneider, 1906 are diploids with 2n = 34, whereas two taxa, S. filipes Handel-Mazzetti,1933 and S. ovalis McAllister, 2005 are tetraploid with 2n = 68. In general, the chromosome size is mainly small, and karyotypes are symmetrical with predominance of metacentric chromosomes. Genome size variation of diploids and tetraploids is 1.401 pg -1.676 pg and 2.674 pg -2.684 pg, respectively. Chromosome numbers of S. amabilis and S. hupehensis var. paucijuga, and karyotype and genome size of eight taxa studied are reported for the first time. This study emphasised the reliability of flow cytometry in genome size determination to infer ploidy levels in Chinese native Sorbus species.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"15 2","pages":"137-148"},"PeriodicalIF":1.0,"publicationDate":"2021-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39034942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Genome size variation and karyotype diversity in eight taxa of Sorbus sensu stricto (Rosaceae) from China 中国蔷薇科8个Sorbus stru stricto分类群基因组大小变异及核型多样性

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-05-20 DOI: 10.3897/compcytogen.v15.i2.58278

Jiabao Li,Kailin Zhu,Qin Wang,Xin Chen

{"title":"Genome size variation and karyotype diversity in eight taxa of Sorbus sensu stricto (Rosaceae) from China","authors":"Jiabao Li,Kailin Zhu,Qin Wang,Xin Chen","doi":"10.3897/compcytogen.v15.i2.58278","DOIUrl":"https://doi.org/10.3897/compcytogen.v15.i2.58278","url":null,"abstract":"Eight taxa of Sorbus Linnaeus, 1753 sensu stricto (Rosaceae) from China have been studied karyologically through chromosome counting, chromosomal measurement and karyotype symmetry. Genome size was also estimated by flow cytometry. Six taxa, S. amabilis Cheng ex T.T.Yu et K.C.Kuan, 1963, S. hupehensis var. paucijuga (D.K. Zang et P.C. Huang, 1992) L.T. Lu, 2000, S. koehneana C.K. Schneider, 1906, S. pohuashanensis (Hance, 1875) Hedlund, 1901, S. scalaris Koehne, 1913 and S. wilsoniana C.K. Schneider, 1906 are diploids with 2n = 34, whereas two taxa, S. filipes Handel-Mazzetti,1933 and S. ovalis McAllister, 2005 are tetraploid with 2n = 68. In general, the chromosome size is mainly small, and karyotypes are symmetrical with predominance of metacentric chromosomes. Genome size variation of diploids and tetraploids is 1.401 pg –1.676 pg and 2.674 pg –2.684 pg, respectively. Chromosome numbers of S. amabilis and S. hupehensis var. paucijuga, and karyotype and genome size of eight taxa studied are reported for the first time. This study emphasised the reliability of flow cytometry in genome size determination to infer ploidy levels in Chinese native Sorbus species.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"25 1","pages":"137-148"},"PeriodicalIF":1.0,"publicationDate":"2021-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138528477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity. 文章标题泰国四种水蚤属植物的核型多样性比较研究。

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-04-26 eCollection Date: 2021-01-01 DOI: 10.3897/CompCytogen.v15i2.60649

Pun Yeesin, Phichaya Buasriyot, Sukhonthip Ditcharoen, Patcharaporn Chaiyasan, Chatmongkon Suwannapoom, Sippakorn Juntaree, Sitthisak Jantarat, Sucheela Talumphai, Marcelo de Bello Cioffi, Thomas Liehr, Alongklod Tanomtong, Weerayuth Supiwong

{"title":"Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity.","authors":"Pun Yeesin, Phichaya Buasriyot, Sukhonthip Ditcharoen, Patcharaporn Chaiyasan, Chatmongkon Suwannapoom, Sippakorn Juntaree, Sitthisak Jantarat, Sucheela Talumphai, Marcelo de Bello Cioffi, Thomas Liehr, Alongklod Tanomtong, Weerayuth Supiwong","doi":"10.3897/CompCytogen.v15i2.60649","DOIUrl":"https://doi.org/10.3897/CompCytogen.v15i2.60649","url":null,"abstract":"Karyotypes of four catfishes of the genus Mystus Scopoli, 1777 (family Bagridae), M. atrifasciatus Fowler, 1937, M. mysticetus Roberts, 1992, M. singaringan (Bleeker, 1846) and M. wolffii (Bleeker, 1851), were analysed by conventional and Ag-NOR banding as well as fluorescence in situ hybridization (FISH) techniques. Microsatellite d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeat probes were applied in FISH. The obtained data revealed that the four studied species have different chromosome complements. The diploid chromosome numbers (2n) and the fundamental numbers (NF) range between 52 and 102, 54 and 104, 56 and 98, or 58 and 108 in M. mysticetus, M. atrifasciatus, M. singaringan or M. wolffii, respectively. Karyotype formulae of M. mysticetus, M. atrifasciatus, M. singaringan and M. wolffii are 24m+26sm+4a, 26m+24sm+2a, 24m+18sm+14a and 30m+22sm+6a, respectively. A single pair of NORs was identified adjacent to the telomeres of the short arm of chromosome pairs 3 (metacentric) in M. atrifasciatus, 20 (submetacentric) in M. mysticetus, 15 (submetacentric) in M. singaringan, and 5 (metacentric) in M. wolffii. The d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeats were abundantly distributed in species-specific patterns. Overall, we present a comparison of cytogenetic and molecular cytogenetic patterns of four species from genus Mystus providing insights into their karyotype diversity in the genus.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"15 2","pages":"119-136"},"PeriodicalIF":1.0,"publicationDate":"2021-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38968063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity 文章标题泰国四种水蚤属植物的核型多样性比较研究

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-04-26 DOI: 10.3897/compcytogen.v15.i2.60649

Pun Yeesin,Phichaya Buasriyot,Sukhonthip Ditcharoen,Patcharaporn Chaiyasan,Chatmongkon Suwannapoom,Sippakorn Juntaree,Sitthisak Jantarat,Sucheela Talumphai,Marcelo de Bello Cioffi,Thomas Liehr,Alongklod Tanomtong,Weerayuth Supiwong

{"title":"Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand: insights into their karyotypic diversity","authors":"Pun Yeesin,Phichaya Buasriyot,Sukhonthip Ditcharoen,Patcharaporn Chaiyasan,Chatmongkon Suwannapoom,Sippakorn Juntaree,Sitthisak Jantarat,Sucheela Talumphai,Marcelo de Bello Cioffi,Thomas Liehr,Alongklod Tanomtong,Weerayuth Supiwong","doi":"10.3897/compcytogen.v15.i2.60649","DOIUrl":"https://doi.org/10.3897/compcytogen.v15.i2.60649","url":null,"abstract":"Karyotypes of four catfishes of the genus Mystus Scopoli, 1777 (family Bagridae), M. atrifasciatus Fowler, 1937, M. mysticetus Roberts, 1992, M. singaringan (Bleeker, 1846) and M. wolffii (Bleeker, 1851), were analysed by conventional and Ag-NOR banding as well as fluorescence in situ hybridization (FISH) techniques. Microsatellite d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeat probes were applied in FISH. The obtained data revealed that the four studied species have different chromosome complements. The diploid chromosome numbers (2n) and the fundamental numbers (NF) range between 52 and 102, 54 and 104, 56 and 98, or 58 and 108 in M. mysticetus, M. atrifasciatus, M. singaringan or M. wolffii, respectively. Karyotype formulae of M. mysticetus, M. atrifasciatus, M. singaringan and M. wolffii are 24m+26sm+4a, 26m+24sm+2a, 24m+18sm+14a and 30m+22sm+6a, respectively. A single pair of NORs was identified adjacent to the telomeres of the short arm of chromosome pairs 3 (metacentric) in M. atrifasciatus, 20 (submetacentric) in M. mysticetus, 15 (submetacentric) in M. singaringan, and 5 (metacentric) in M. wolffii. The d(GC)15, d(CAA)10, d(CAT)10 and d(GAA)10 repeats were abundantly distributed in species-specific patterns. Overall, we present a comparison of cytogenetic and molecular cytogenetic patterns of four species from genus Mystus providing insights into their karyotype diversity in the genus.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"43 1","pages":"119-136"},"PeriodicalIF":1.0,"publicationDate":"2021-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138528476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and physical localization of male-biased repetitive DNA sequences in Spinacia oleracea (Amaranthaceae). 苋科植物菠菜雄性偏置重复DNA序列的克隆与物理定位。

IF 1 4区生物学

Comparative Cytogenetics Pub Date : 2021-04-23 eCollection Date: 2021-01-01 DOI: 10.3897/CompCytogen.v15i2.63061

Jian Zhou, Shaojing Wang, Li'ang Yu, Ning Li, Shufen Li, Yulan Zhang, Ruiyun Qin, Wujun Gao, Chuanliang Deng

{"title":"Cloning and physical localization of male-biased repetitive DNA sequences in Spinacia oleracea (Amaranthaceae).","authors":"Jian Zhou, Shaojing Wang, Li'ang Yu, Ning Li, Shufen Li, Yulan Zhang, Ruiyun Qin, Wujun Gao, Chuanliang Deng","doi":"10.3897/CompCytogen.v15i2.63061","DOIUrl":"https://doi.org/10.3897/CompCytogen.v15i2.63061","url":null,"abstract":"Spinach (Spinacia oleracea Linnaeus, 1753) is an ideal material for studying molecular mechanisms of early-stage sex chromosome evolution in dioecious plants. Degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) technique facilitates the retrotransposon-relevant studies by enriching specific repetitive DNA sequences from a micro-dissected single chromosome. We conducted genomic subtractive hybridization to screen sex-biased DNA sequences by using the DOP-PCR amplification products of micro-dissected spinach Y chromosome. The screening yielded 55 male-biased DNA sequences with 30 576 bp in length, of which, 32 DNA sequences (12 049 bp) contained repeat DNA sequences, including LTR/Copia, LTR/Gypsy, simple repeats, and DNA/CMC-EnSpm. Among these repetitive DNA sequences, four DNA sequences that contained a fragment of Ty3-gypsy retrotransposons (SP73, SP75, SP76, and SP77) were selected as fluorescence probes to hybridization on male and female spinach karyotypes. Fluorescence in situ hybridization (FISH) signals of SP73 and SP75 were captured mostly on the centromeres and their surrounding area for each homolog. Hybridization signals primarily appeared near the putative centromeres for each homologous chromosome pair by using SP76 and SP77 probes for FISH, and sporadic signals existed on the long arms. Results can be served as a basis to study the function of repetitive DNA sequences in sex chromosome evolution in spinach.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"15 2","pages":"101-118"},"PeriodicalIF":1.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38968062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2