Comparative Cytogenetics最新文献

{"title":"Distribution patterns of rDNA loci in the Schedonorus-Lolium complex (Poaceae)","authors":"H. Ansari, N. Ellison, A. Stewart, Warren Mervyn Williams","doi":"10.3897/compcytogen.v16.i1.79056","DOIUrl":"https://doi.org/10.3897/compcytogen.v16.i1.79056","url":null,"abstract":"Abstract The Schedonorus-Lolium complex of the subtribe Loliinae (Poaceae) includes several economically important forage and turf grasses. This complex encompasses Lolium Linnaeus, 1753, Festuca Linnaeus, 1753 subgenus Schedonorus (P. Beauvois, 1824) Petermann, 1849 and Micropyropsis Romero Zarco et Cabezudo, 1983. New FISH results of 5S and 18S–26S rDNA sequences are presented for three species and the results are interpreted in a review of distribution patterns of 5S and 18S–26S rDNA sequences among other species in the complex. Micropyropsistuberosa Romero Zarco et Cabezudo, 1983 (2n = 2x = 14) displayed a distribution pattern of rDNA sequences identical to that of F.pratensis Hudson, 1762, supporting a close phylogenetic relationship at the bottom of the phylogenetic tree. “Loliummultiflorum” Lamarck, 1779 accessions sourced from Morocco showed a different pattern from European L.multiflorum and could be a unique and previously uncharacterised taxon. North African Festucasimensis Hochstetter ex A. Richard, 1851 had a marker pattern consistent with allotetraploidy and uniparental loss of one 18S–26S rDNA locus. This allotetraploid has previously been suggested to have originated from a hybrid with Festucaglaucescens (Festucaarundinaceavar.glaucescens Boissier, 1844). However, the distribution patterns of the two rDNA sequences in this allotetraploid do not align with F.glaucescens, suggesting that its origin from this species is unlikely. Furthermore, comparisons with other higher alloploids in the complex indicate that F.simensis was a potential donor of two sub-genomes of allohexaploid Festucagigantea (Linnaeus) Villars, 1787. In the overall complex, the proximal locations of both rDNA markers were conserved among the diploid species. Two types of synteny of the two markers could, to a considerable extent, distinguish allo- and autogamous Lolium species. The ancestral parentage of the three Festuca allotetraploids has not yet been determined, but all three appear to have been sub-genome donors to the higher allopolypoids of sub-genus Schedonorus. Terminal locations of both the markers were absent from the diploids but were very frequently observed in the polyploids.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 1","pages":"39 - 54"},"PeriodicalIF":1.0,"publicationDate":"2022-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43639719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Karyotype characteristics and gene COI sequences of Chironomusbonus Shilova et Dzhvarsheishvili, 1974 (Diptera, Chironomidae) from the South Caucasus (Republic of Georgia, Paravani river)","authors":"M. K. Karmokov","doi":"10.3897/CompCytogen.v16.i1.79182","DOIUrl":"https://doi.org/10.3897/CompCytogen.v16.i1.79182","url":null,"abstract":"Abstract","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 1","pages":"19 - 38"},"PeriodicalIF":1.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47371354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily.","authors":"Marcello Mezzasalma,&nbsp;Franco Andreone,&nbsp;Gaetano Odierna,&nbsp;Fabio Maria Guarino,&nbsp;Angelica Crottini","doi":"10.3897/compcytogen.v16.i1.76260","DOIUrl":"10.3897/compcytogen.v16.i1.76260","url":null,"abstract":"<p><p>We performed a molecular and cytogenetic analysis on different Mantellinae species and revised the available chromosomal data on this group to provide an updated assessment of its karyological diversity and evolution. Using a fragment of the mitochondrial 16S rRNA, we performed a molecular taxonomic identification of the samples that were used for cytogenetic analyses. A comparative cytogenetic analysis, with Giemsa's staining, Ag-NOR staining and sequential C-banding + Giemsa + CMA + DAPI was performed on eight species: <i>Gephyromantis</i> sp. Ca19, <i>G.striatus</i> (Vences, Glaw, Andreone, Jesu et Schimmenti, 2002), Mantidactylus (Chonomantis) sp. Ca11, M. (Brygoomantis) alutus (Peracca, 1893), M. (Hylobatrachus) cowanii (Boulenger, 1882), Spinomantispropeaglavei \"North\" (Methuen et Hewitt, 1913), <i>S.phantasticus</i> (Glaw et Vences, 1997) and <i>S.</i> sp. Ca3. <i>Gephyromantisstriatus</i>, M. (Brygoomantis) alutus and Spinomantispropeaglavei \"North\" have a karyotype of 2n = 24 chromosomes while the other species show 2n = 26 chromosomes. Among the analysed species we detected differences in the number and position of telocentric elements, location of NOR loci (alternatively on the 6^th, 7^th or 10^th pair) and in the distribution of heterochromatin, which shows species-specific patterns. Merging our data with those previously available, we propose a karyotype of 2n = 26 with all biarmed elements and loci of NORs on the 6^th chromosome pair as the ancestral state in the whole family Mantellidae. From this putative ancestral condition, a reduction of chromosome number through similar tandem fusions (from 2n = 26 to 2n = 24) occurred independently in Mantidactylus Boulenger, 1895 (subgenus Brygoomantis Dubois, 1992), <i>Spinomantis</i> Dubois, 1992 and <i>Gephyromantis</i> Methuen, 1920. Similarly, a relocation of NORs, from the putative primitive configuration on the 6^th chromosome, occurred independently in <i>Gephyromantis</i>, <i>Blommersia</i> Dubois, 1992, <i>Guibemantis</i> Dubois, 1992, <i>Mantella</i> Boulenger, 1882 and <i>Spinomantis</i>. Chromosome inversions of primitive biarmed elements likely generated a variable number of telocentric elements in <i>Mantellanigricans</i> Guibé, 1978 and a different number of taxa of <i>Gephyromantis</i> (subgenera <i>Duboimantis</i> Glaw et Vences, 2006 and <i>Laurentomantis</i> Dubois, 1980) and <i>Mantidactylus</i> (subgenera <i>Brygoomantis</i>, <i>Chonomantis</i> Glaw et Vences, 1994, <i>Hylobatrachus</i> Laurent, 1943 and <i>Ochthomantis</i> Glaw et Vences, 1994).</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 1","pages":"1-17"},"PeriodicalIF":1.0,"publicationDate":"2022-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39670842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First cytogenetic data on Afrotropical lutefishes (Citharinidae) in the light of karyotype evolution in Characiformes.","authors":"Sergey A Simanovsky,&nbsp;Dmitry A Medvedev,&nbsp;Fekadu Tefera,&nbsp;Alexander S Golubtsov","doi":"10.3897/compcytogen.v16.i2.79133","DOIUrl":"https://doi.org/10.3897/compcytogen.v16.i2.79133","url":null,"abstract":"<p><p>The Afrotropical lutefish family Citharinidae (Citharinoidei, Characiformes) comprises three genera with eight species in total. Although Citharinidae have been studied in terms of taxonomy and systematics, no cytogenetic information was available for any representative of the family. Furthermore, only one species out of 116 in Citharinoidei (<i>Distichodusaffinis</i> Günther, 1873) has been studied cytogenetically. Here, we report the karyotypes of <i>Citharinuscitharus</i> (Geoffroy St. Hilaire, 1809) from West Africa and <i>Citharinuslatus</i> Müller et Troschel, 1844 from Northeast Africa. The former has the diploid chromosome number 2n = 40 and the fundamental number FN = 80, while the latter has 2n = 44 and FN = 88. Hence, these karyotypes consist exclusively of bi-armed chromosomes. Such karyotypes were previously found in <i>D.affinis</i> and in many lineages of Neotropical species of another suborder of Characiformes, Characoidei. In contrast, the karyotypes dominated by uni-armed elements are typical for a number of phylogenetically basal lineages of Afrotropical and Neotropical Characoidei. We discuss the importance of our data on Citharinidae for the understanding of the karyotype evolution within the order Characiformes.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 2","pages":"143-150"},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10694314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A critical review on cytogenetics of Cucurbitaceae with updates on Indian taxa.","authors":"Biplab Kumar Bhowmick,&nbsp;Sumita Jha","doi":"10.3897/compcytogen.v16.i2.79033","DOIUrl":"https://doi.org/10.3897/compcytogen.v16.i2.79033","url":null,"abstract":"<p><p>The cytogenetic relationships in the species of Cucurbitaceae are becoming immensely important to answer questions pertaining to genome evolution. Here, a simplified and updated data resource on cytogenetics of Cucurbitaceae is presented on the basis of foundational parameters (basic, zygotic and gametic chromosome numbers, ploidy, genome size, karyotype) and molecular cytogenetics. We have revised and collated our own findings on seven agriculturally important Indian cucurbit species in a comparative account with the globally published reports. Chromosome count (of around 19% species) shows nearly three-fold differences while genome size (of nearly 5% species) shows 5.84-fold differences across the species. There is no significant correlation between chromosome numbers and nuclear genome sizes. The possible trend of evolution is discussed here based on molecular cytogenetics data, especially the types and distribution of nucleolus organizer regions (NORs). The review supersedes the scopes of general chromosome databases and invites scopes for continuous updates. The offline resource serves as an exclusive toolkit for research and breeding communities across the globe and also opens scope for future establishment of web-database on Cucurbitaceae cytogenetics.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 2","pages":"93-125"},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10694319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Karyotype description and comparative chromosomal mapping of rDNA and U2 snDNA sequences in <i>Eigenmannialimbata</i> and <i>E.microstoma</i> (Teleostei, Gymnotiformes, Sternopygidae).","authors":"Cristian Andrés Araya-Jaime,&nbsp;Duílio Mazzoni Zerbinato de Andrade Silva,&nbsp;Luís Ricardo Ribeiro da Silva,&nbsp;Cristiano Neves do Nascimento,&nbsp;Claudio Oliveira,&nbsp;Fausto Foresti","doi":"10.3897/CompCytogen.v16i2.72190","DOIUrl":"https://doi.org/10.3897/CompCytogen.v16i2.72190","url":null,"abstract":"<p><p>The genus <i>Eigenmannia</i> Jordan et Evermann,1896 includes electric fishes endemic to the Neotropical region with extensive karyotype variability and occurrence of different sex chromosome systems, however, cytogenetic studies within this group are restricted to few species. Here, we describe the karyotypes of <i>Eigenmannialimbata</i> (Schreiner et Miranda Ribeiro, 1903) and <i>E.microstoma</i> (Reinhardt, 1852) and the chromosomal locations of 5S and 18S rDNAs (ribosomal RNA genes) and U2 snDNA (small nuclear RNA gene). Among them, 18S rDNA sites were situated in only one chromosomal pair in both species, and co-localized with 5S rDNA in <i>E.microstoma</i>. On the other hand, 5S rDNA and U2 snRNA sites were observed on several chromosomes, with variation in the number of sites between species under study. These two repetitive DNAs were observed co-localized in one chromosomal pair in <i>E.limbata</i> and in four pairs in <i>E.microstoma</i>. Our study shows a new case of association of these two types of repetitive DNA in the genome of Gymnotiformes.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 2","pages":"127-142"},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10685851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The new highest number of B chromosomes (Bs) in Leisler's bat <i>Nyctalusleisleri</i> (Kuhl, 1817).","authors":"Marija Rajičić,&nbsp;Ivana Budinski,&nbsp;Milan Miljević,&nbsp;Branka Bajić,&nbsp;Milan Paunović,&nbsp;Mladen Vujošević,&nbsp;Jelena Blagojević","doi":"10.3897/CompCytogen.v16i3.89911","DOIUrl":"https://doi.org/10.3897/CompCytogen.v16i3.89911","url":null,"abstract":"<p><p>B chromosomes (Bs) are supernumerary to the standard chromosome set, from which they prevalently derive. Variation in numbers both among individuals or populations and among cells within individuals is their constant feature. Leisler's bat <i>Nyctalusleisleri</i> (Kuhl, 1817) is one of only four species of Chiroptera with detected Bs. Four males of <i>N.leisleri</i> were collected from two localities on the territory of Serbia and cytogenetically analysed. All animals had Bs with interindividual variability ranging from two to five heterochromatic micro Bs. The highest number of Bs was detected in this species. Among mammals, Rodentia and Chiroptera are orders with the largest number of species, but Bs frequently appear in rodents and rarely in chiropterans. Possible explanations for this difference are offered.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 3","pages":"173-184"},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9256954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative analysis reveals cryptic speciation linked to habitat differentiation within Albanian populations of the anomalous blues (Lepidoptera, Lycaenidae, <i>Polyommatus</i> Latreille, 1804).","authors":"Laurian Parmentier,&nbsp;Roger Vila,&nbsp;Vladimir Lukhtanov","doi":"10.3897/CompCytogen.v16.i4.90558","DOIUrl":"https://doi.org/10.3897/CompCytogen.v16.i4.90558","url":null,"abstract":"<p><p>The Balkan Peninsula is one of the greatest hotspots for biodiversity in Europe. While the region has been investigated thoroughly, some parts remain understudied and may still harbour undiscovered diversity, even in well-studied organisms such as Lepidoptera. Here we investigated the group of the so-called anomalous blue butterflies, also known as 'brown complex' of the subgenus Agrodiaetus Hübner, 1822 including the taxa of the entire <i>Polyommatusaroaniensis</i> (Brown, 1976) species complex. This species complex is distributed in the southern part of the Balkan Peninsula and known to be represented by three closely related allopatric species, differentiated by their chromosome numbers (n) and mitochondrial (mt) DNA. These are <i>P.aroaniensis</i> sensu stricto (Southern Greece, Peloponnese, n=47-48; mt haplogroup <i>aroa1</i>), <i>P.timfristos</i> Lukhtanov, Vishnevskaya et Shapoval, 2016 (Central Greece, Attika, n=38, <i>aroa2</i>) and <i>P.orphicus</i> Kolev, 2005 (North-Eastern Greece, Southern Bulgaria, n=41-42, <i>orph1</i>). Based on an analysis of chromosomal, molecular and morphological markers, we demonstrate that a fourth taxon of this species complex exists in Albania. This taxon possesses the mt haplogroup <i>aroa3</i>, which is the most differentiated within the entire <i>P.aroaniensis</i> species complex, and the karyotype (n=42-43), which differs by one fixed chromosome fission from <i>P.orphicus</i>. The Albanian taxon seems to be ecologically specialised (habitat on dark-coloured, ophiolitic substrate soils) and differs in colouration (wing reflectance) from the others taxa of the <i>P.aroaniensis</i> species group. Based on the evidence here presented and following the current view of the taxonomy of the group, we propose considering the Albanian taxon as a new species, here described as <i>Polyommatuslurae</i> <b>sp. nov.</b> At the contact zone between the new species and <i>P.orphicus</i>, in addition to typical ones, we detected specimens with haplogroup orph2, karyotype n=43 and intermediate morphology, which seem to represent <i>P.lurae</i> × <i>P.orphicus</i> hybrids.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 4","pages":"211-242"},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10687551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Karyotype differentiation and male meiosis in European clades of the spider genus <i>Pholcus</i> (Araneae, Pholcidae).","authors":"Jiří Král,&nbsp;Ivalú M Ávila Herrera,&nbsp;František Šťáhlavský,&nbsp;David Sadílek,&nbsp;Jaroslav Pavelka,&nbsp;Maria Chatzaki,&nbsp;Bernhard A Huber","doi":"10.3897/CompCytogen.v16i4.85059","DOIUrl":"https://doi.org/10.3897/CompCytogen.v16i4.85059","url":null,"abstract":"<p><p>Haplogyne araneomorphs are a diverse spider clade. Their karyotypes are usually predominated by biarmed (i.e., metacentric and submetacentric) chromosomes and have a specific sex chromosome system, X₁X₂Y. These features are probably ancestral for haplogynes. Nucleolus organizer regions (NORs) spread frequently from autosomes to sex chromosomes in these spiders. This study focuses on pholcids (Pholcidae), a highly diverse haplogyne family. Despite considerable recent progress in pholcid cytogenetics, knowledge on many clades remains insufficient including the most species-rich pholcid genus, <i>Pholcus</i> Walckenaer, 1805. To characterize the karyotype differentiation of <i>Pholcus</i> in Europe, we compared karyotypes, sex chromosomes, NORs, and male meiosis of seven species [<i>P.alticeps</i> Spassky, 1932; <i>P.creticus</i> Senglet, 1971; <i>P.dentatus</i> Wunderlich, 1995; <i>P.fuerteventurensis</i> Wunderlich, 1992; <i>P.phalangioides</i> (Fuesslin, 1775); <i>P.opilionoides</i> (Schrank, 1781); <i>P.silvai</i> Wunderlich, 1995] representing the dominant species groups in this region. The species studied show several features ancestral for <i>Pholcus</i>, namely the 2n♂ = 25, the X₁X₂Y system, and a karyotype predominated by biarmed chromosomes. Most taxa have a large acrocentric NOR-bearing pair, which evolved from a biarmed pair by a pericentric inversion. In some lineages, the acrocentric pair reverted to biarmed. Closely related species often differ in the morphology of some chromosome pairs, probably resulting from pericentric inversions and/or translocations. Such rearrangements have been implicated in the formation of reproductive barriers. While the X₁ and Y chromosomes retain their ancestral metacentric morphology, the X₂ chromosome shows a derived (acrocentric or subtelocentric) morphology. Pairing of this element is usually modified during male meiosis. NOR patterns are very diverse. The ancestral karyotype of <i>Pholcus</i> contained five or six terminal NORs including three X chromosome-linked loci. The number of NORs has been frequently reduced during evolution. In the Macaronesian clade, there is only a single NOR-bearing pair. Sex chromosome-linked NORs are lost in Madeiran species and in <i>P.creticus</i>. Our study revealed two cytotypes in the synanthropic species <i>P.phalangioides</i> (Madeiran and Czech), which differ by their NOR pattern and chromosome morphology. In the Czech cytotype, the large acrocentric pair was transformed into a biarmed pair by pericentric inversion.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 4","pages":"185-209"},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10687554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Karyotype and COI gene sequences of <i>Chironomusmelanotus</i> Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems.","authors":"Viktor V Bolshakov,&nbsp;Ekaterina A Movergoz","doi":"10.3897/compcytogen.v16.i3.90336","DOIUrl":"https://doi.org/10.3897/compcytogen.v16.i3.90336","url":null,"abstract":"<p><p>Karyotype and <i>COI</i> gene sequences of <i>Chironomusmelanotus</i> Keyl, 1961 from the Yaroslavl region (Russia) were analyzed. A low level of chromosomal polymorphism has been confirmed, eventually eight banding sequences were found: melA1, melB1, melC1, melD1, melE1, melF1, and melG1; only melD2 was found in two larvae from the Sunoga river. Analysis of phylogenetic tree and estimated genetic distances has shown not all <i>COI</i> gene sequences of <i>Ch.melanotus</i> in GenBank and BOLD to belong to this species. The lower distance of 0.4% was observed between two sequences from the Yaroslavl region and Finland, apparently these are true <i>Ch.melanotus</i> sequences. The distances between true <i>Ch.melanotus</i> and other sequences from Finland were 9.5% and 12.4%, and from Sweden it was 11%. The average genetic distance between studied sequences of 9.1% is out of the range of the 3% threshold previously determined for chironomids. According to our estimates, there are two sequences with a distance of 2.9% that may belong to <i>Ch.annularius</i> Meigen, 1818, and one sequence with a genetic distance of 2.1%, may belonging to <i>Ch.cingulatus</i> Meigen, 1830, which has been confirmed karyologically. Another two sequences form a separate cluster. We suggest that they either belong to a known species, but are not present in the databases, or belong to a distinct, undescribed species.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 3","pages":"161-172"},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10689446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}