Comparative Cytogenetics最新文献

{"title":"Assessing ploidy levels and karyotype structure of the fire ant <i>Solenopsissaevissima</i> Smith, 1855 (Hymenoptera, Formicidae, Myrmicinae).","authors":"Ananda Ribeiro Macedo de Andrade, Danon Clemes Cardoso, Maykon Passos Cristiano","doi":"10.3897/compcytogen.17.100945","DOIUrl":"10.3897/compcytogen.17.100945","url":null,"abstract":"<p><p>The family Formicidae is composed of ants that organize themselves into castes in which every individual has a joint organizational function. <i>Solenopsis</i> Westwood, 1840 is an ant genus with opportunistic and aggressive characteristics, known for being invasive species and stings that cause burning in humans. This genus is particularly difficult to classify and identify since its morphology provides few indications for species differentiation. For this, a tool that has been useful for evolutionary and taxonomic studies is cytogenetics. Here, we cytogenetically studied <i>Solenopsissaevissima</i> Smith, 1855 from Ouro Preto, Minas Gerais, Brazil. We evaluated the occurrence of polyploid cells in individuals and colonies by conventional cytogenetics. A total of 450 metaphases were analyzed and counted. Chromosome counts of individuals and colonies showed varied numbers of ploidies, from n = 16 to 8n = 128. The karyomorphometrical approach allowed determination of the following karyotypes: n = 10 m + 4 sm + 2 st, 2n = 20 m + 8 sm + 4 st, and 4n = 40 m + 16 sm + 8 st. Polyploidy can be found naturally in individuals and colonies and may represent an adaptative trait related to widespread distribution and invasion ability of new habitats.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"1 1","pages":"59-73"},"PeriodicalIF":1.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45560377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative karyotype analysis of eight Cucurbitaceae crops using fluorochrome banding and 45S rDNA-FISH.","authors":"Chao-Wen She, Xiang-Hui Jiang, Chun-Ping He","doi":"10.3897/compcytogen.v17.i1.99236","DOIUrl":"10.3897/compcytogen.v17.i1.99236","url":null,"abstract":"<p><p>To have an insight into the karyotype variation of eight Cucurbitaceae crops including <i>Cucumissativus</i> Linnaeus, 1753, <i>Cucumismelo</i> Linnaeus, 1753, <i>Citrulluslanatus</i> (Thunberg, 1794) Matsumura et Nakai, 1916, <i>Benincasahispida</i> (Thunberg, 1784) Cogniaux, 1881, <i>Momordicacharantia</i> Linnaeus, 1753, <i>Luffacylindrica</i> (Linnaeus, 1753) Roemer, 1846, Lagenariasicerariavar.hispida (Thunberg, 1783) Hara, 1948 and <i>Cucurbitamoschata</i> Duchesne ex Poiret, 1819, well morphologically differentiated mitotic metaphase chromosomes were prepared using the enzymatic maceration and flame-drying method, and the chromosomal distribution of heterochromatin and 18S-5.8S-26S rRNA genes (45S rDNA) was investigated using sequential combined PI and DAPI (CPD) staining and fluorescence <i>in situ</i> hybridization (FISH) with 45S rDNA probe. Detailed karyotypes were established using the dataset of chromosome measurements, fluorochrome bands and rDNA FISH signals. Four karyotype asymmetry indices, CV_CI, CV_CL, M_CA and Stebbins' category, were measured to elucidate the karyological relationships among species. All the species studied had symmetrical karyotypes composed of metacentric and submetacentric or only metacentric chromosomes, but their karyotype structure can be discriminated by the scatter plot of M_CA vs. CV_CL. The karyological relationships among these species revealed by PCoA based on <i>x</i>, 2<i>n</i>, TCL, M_CA, CV_CL and CV_CI was basically in agreement with the phylogenetic relationships revealed by DNA sequences. CPD staining revealed all 45S rDNA sites in all species, (peri)centromeric GC-rich heterochromatin in <i>C.sativus</i>, <i>C.melo</i>, <i>C.lanatus</i>, <i>M.charantia</i> and <i>L.cylindrica</i>, terminal GC-rich heterochromatin in <i>C.sativus</i>. DAPI counterstaining after FISH revealed pericentromeric DAPI⁺ heterochromatin in <i>C.moschata</i>. rDNA FISH detected two 45S loci in five species and five 45S loci in three species. Among these 45S loci, most were located at the terminals of chromosome arms, and a few in the proximal regions. In <i>C.sativus</i>, individual chromosomes can be precisely distinguished by the CPD band and 45S rDNA signal patterns, providing an easy method for chromosome identification of cucumber. The genome differentiation among these species was discussed in terms of genome size, heterochromatin, 45S rDNA site, and karyotype asymmetry based on the data of this study and previous reports.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"17 1","pages":"31-58"},"PeriodicalIF":1.0,"publicationDate":"2023-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9994029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Karyotype differentiation in the <i>Nothobranchiusugandensis</i> species group (Teleostei, Cyprinodontiformes), seasonal fishes from the east African inland plateau, in the context of phylogeny and biogeography.","authors":"Eugene Yu Krysanov, Béla Nagy, Brian R Watters, Alexandr Sember, Sergey A Simanovsky","doi":"10.3897/compcytogen.v7.i1.97165","DOIUrl":"10.3897/compcytogen.v7.i1.97165","url":null,"abstract":"<p><p>The karyotype differentiation of the twelve known members of the <i>Nothobranchiusugandensis</i> Wildekamp, 1994 species group is reviewed and the karyotype composition of seven of its species is described herein for the first time using a conventional cytogenetic protocol. Changes in the architecture of eukaryotic genomes often have a major impact on processes underlying reproductive isolation, adaptation and diversification. African annual killifishes of the genus <i>Nothobranchius</i> Peters, 1868 (Teleostei: Nothobranchiidae), which are adapted to an extreme environment of ephemeral wetland pools in African savannahs, feature extensive karyotype evolution in small, isolated populations and thus are suitable models for studying the interplay between karyotype change and species evolution. The present investigation reveals a highly conserved diploid chromosome number (2n = 36) but a variable number of chromosomal arms (46-64) among members of the <i>N.ugandensis</i> species group, implying a significant role of pericentric inversions and/or other types of centromeric shift in the karyotype evolution of the group. When superimposed onto a phylogenetic tree based on molecular analyses of two mitochondrial genes the cytogenetic characteristics did not show any correlation with the phylogenetic relationships within the lineage. While karyotypes of many other <i>Nothobranchius</i> spp. studied to date diversified mainly via chromosome fusions and fissions, the <i>N.ugandensis</i> species group maintains stable 2n and the karyotype differentiation seems to be constrained to intrachromosomal rearrangements. Possible reasons for this difference in the trajectory of karyotype differentiation are discussed. While genetic drift seems to be a major factor in the fixation of chromosome rearrangements in <i>Nothobranchius</i>, future studies are needed to assess the impact of predicted multiple inversions on the genome evolution and species diversification within the <i>N.ugandensis</i> species group.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"17 1","pages":"13-29"},"PeriodicalIF":1.0,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9620493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome complements of <i>Channalucius</i> and <i>C.striata</i> from Phu Quoc Island and karyotypic evolution in snakehead fishes (Actinopterygii, Channidae).","authors":"Denis V Prazdnikov","doi":"10.3897/compcytogen.v17.i1.94943","DOIUrl":"10.3897/compcytogen.v17.i1.94943","url":null,"abstract":"<p><p>Snakehead fishes of the family Channidae are obligatory air-breathers freshwater predators, the vast majority of which belong to the genus <i>Channa</i> Scopoli, 1777. <i>Channa</i> species are characterized by high karyotypic diversity due to various types of chromosomal rearrangements. It is assumed that, in addition to the lifestyle, fragmentation and isolation of snakehead populations contribute to an increase in karyotypic diversity. However, the chromosome complements of many isolated populations of widespread <i>Channa</i> species remain unknown, and the direction of karyotype transformations is poorly understood. This paper describes the previously unstudied karyotypes of <i>Channalucius</i> (Cuvier, 1831) and <i>C.striata</i> (Bloch, 1793) from Phu Quoc Island and analyzes the trends of karyotypic evolution in the genus <i>Channa</i>. In <i>C.lucius</i>, the karyotypes are differed in the number of chromosome arms (2n = 48, NF = 50 and 51), while in <i>C.striata</i>, the karyotypes are differed in the diploid chromosome number (2n = 44 and 43, NF = 48). A comparative cytogenetic analysis showed that the main trend of karyotypic evolution of <i>Channa</i> species is associated with a decrease in the number of chromosomes and an increase in the number of chromosome arms, mainly due to fusions and pericentric inversions. The data obtained support the assumption that fragmentation and isolation of populations, especially of continental islands, contribute to the karyotypic diversification of snakeheads and are of interest for further cytogenetic studies of Channidae.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"17 1","pages":"1-12"},"PeriodicalIF":1.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9836404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10693821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Karyotype of <i>Sabanejewiabulgarica</i> (Drensky, 1928) (Teleostei, Cobitidae) from the Danube Delta, Romania.","authors":"Eva Hnátková,&nbsp;Zuzana Majtánová,&nbsp;Vendula Bohlen Šlechtová,&nbsp;Joerg Bohlen,&nbsp;Petr Ráb","doi":"10.3897/compcytogen.17.103152","DOIUrl":"https://doi.org/10.3897/compcytogen.17.103152","url":null,"abstract":"<p><p>The karyotype of the freshwater fish <i>Sabanejewiabulgarica</i> (Drensky, 1928), from the Danube Delta, was studied by conventional Giemsa staining and the C-banding technique. The diploid chromosome number was 2n = 50. The karyotype contained 2 pairs of metacentric (the first one was much larger than the second one), 6 pairs of submetacentric and 17 pairs of subtelocentric to acrocentric chromosomes. Pericentromeric blocks of heterochromatin were revealed in most of the chromosome pairs. The karyotype phenotype of <i>S.bulgarica</i> was the same as found for <i>S.balcanica</i> from Northern Carpathian Mountains.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"17 ","pages":"157-162"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10353547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9899411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"More hidden diversity in a cryptic species complex: a new subspecies of <i>Leptideasinapis</i> (Lepidoptera, Pieridae) from Northern Iran.","authors":"Vazrick Nazari,&nbsp;Vladimir A Lukhtanov,&nbsp;Alireza Naderi,&nbsp;Zdenek Faltýnek Fric,&nbsp;Vlad Dincă,&nbsp;Roger Vila","doi":"10.3897/compcytogen.17.102830","DOIUrl":"https://doi.org/10.3897/compcytogen.17.102830","url":null,"abstract":"<p><p>A new subspecies of <i>Leptideasinapis</i> from Northern Iran, discovered by means of DNA barcoding, is described as <i>Leptideasinapistabarestana</i><b>ssp. nov.</b> The new subspecies is allopatric with respect to other populations of <i>L.sinapis</i> and is genetically distinct, appearing as a well-supported sister clade to all other populations in COI-based phylogenetic reconstructions. Details on karyotype, genitalia, ecology and behaviour for the new subspecies are given and a biogeographical speciation scenario is proposed.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"17 ","pages":"113-128"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9617619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Allium</i> cytogenetics: a critical review on the Indian taxa.","authors":"Biplab Kumar Bhowmick,&nbsp;Sayantika Sarkar,&nbsp;Dipasree Roychowdhury,&nbsp;Sayali D Patil,&nbsp;Manoj M Lekhak,&nbsp;Deepak Ohri,&nbsp;Satyawada Rama Rao,&nbsp;S R Yadav,&nbsp;R C Verma,&nbsp;Manoj K Dhar,&nbsp;S N Raina,&nbsp;Sumita Jha","doi":"10.3897/CompCytogen.17.98903","DOIUrl":"https://doi.org/10.3897/CompCytogen.17.98903","url":null,"abstract":"<p><p>The genus <i>Allium</i> Linnaeus, 1753 (tribe Allieae) contains about 800 species worldwide of which almost 38 species are reported in India, including the globally important crops (onion, garlic, leek, shallot) and many wild species. A satisfactory chromosomal catalogue of <i>Allium</i> species is missing which has been considered in the review for the species occurring in India. The most prominent base number is x=8, with few records of x=7, 10, 11. The genome size has sufficient clues for divergence, ranging from 7.8 pg/1C to 30.0 pg/1C in diploid and 15.16 pg/1C to 41.78 pg/1C in polyploid species. Although the karyotypes are seemingly dominated by metacentrics, substantial variation in nucleolus organizing regions (NORs) is noteworthy. The chromosomal rearrangement between <i>A.cepa</i> Linnaeus, 1753 and its allied species has paved way to appreciate genomic evolution within <i>Allium</i>. The presence of a unique telomere sequence and its conservation in <i>Allium</i> sets this genus apart from all other Amaryllids and supports monophyletic origin. Any cytogenetic investigation regarding NOR variability, telomere sequence and genome size in the Indian species becomes the most promising field to decipher chromosome evolution against the background of species diversity and evolution, especially in the Indian subcontinent.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"17 ","pages":"129-156"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9617621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraspecific divergence of diploid grass <i>Aegilopscomosa</i> is associated with structural chromosome changes.","authors":"Ekaterina D Badaeva,&nbsp;Violetta V Kotseruba,&nbsp;Andnrey V Fisenko,&nbsp;Nadezhda N Chikida,&nbsp;Maria Kh Belousova,&nbsp;Peter M Zhurbenko,&nbsp;Sergei A Surzhikov,&nbsp;Alexandra Yu Dragovich","doi":"10.3897/CompCytogen.17.101008","DOIUrl":"https://doi.org/10.3897/CompCytogen.17.101008","url":null,"abstract":"<p><p><i>Aegilopscomosa</i> Smith in Sibthorp et Smith, 1806 is diploid grass with MM genome constitution occurring mainly in Greece. Two morphologically distinct subspecies - <i>Ae.c.comosa</i> Chennaveeraiah, 1960 and <i>Ae.c.heldreichii</i> (Holzmann ex Boissier) Eig, 1929 are discriminated within <i>Ae.comosa</i>, however, genetic and karyotypic bases of their divergence are not fully understood. We used Fluorescence in situ hybridization (FISH) with repetitive DNA probes and electrophoretic analysis of gliadins to characterize the genome and karyotype of <i>Ae.comosa</i> to assess the level of their genetic diversity and uncover mechanisms leading to radiation of subspecies. We show that two subspecies differ in size and morphology of chromosomes 3M and 6M, which can be due to reciprocal translocation. Subspecies also differ in the amount and distribution of microsatellite and satellite DNA sequences, the number and position of minor NORs, especially on 3M and 6M, and gliadin spectra mainly in the a-zone. Frequent occurrence of hybrids can be caused by open pollination, which, along with genetic heterogeneity of accessions and, probably, the lack of geographic or genetic barrier between the subspecies, may contribute to extremely broad intraspecific variation of GAA_n and gliadin patterns in <i>Ae.comosa</i>, which are usually not observed in endemic plant species.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"17 ","pages":"75-112"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10252141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9620726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete chloroplast genome sequence of <i>Rhododendronmariesii</i> and comparative genomics of related species in the family Ericaeae.","authors":"Zhiliang Li,&nbsp;Zhiwei Huang,&nbsp;Xuchun Wan,&nbsp;Jiaojun Yu,&nbsp;Hongjin Dong,&nbsp;Jialiang Zhang,&nbsp;Chunyu Zhang,&nbsp;Shuzhen Wang","doi":"10.3897/compcytogen.17.101427","DOIUrl":"https://doi.org/10.3897/compcytogen.17.101427","url":null,"abstract":"<p><p><i>Rhododendronmariesii</i> Hemsley et Wilson, 1907, a typical member of the family Ericaeae, possesses valuable medicinal and horticultural properties. In this research, the complete chloroplast (cp) genome of <i>R.mariesii</i> was sequenced and assembled, which proved to be a typical quadripartite structure with the length of 203,480 bp. In particular, the lengths of the large single copy region (LSC), small single copy region (SSC), and inverted repeat regions (IR) were 113,715 bp, 7,953 bp, and 40,918 bp, respectively. Among the 151 unique genes, 98 were protein-coding genes, 8 were tRNA genes, and 45 were rRNA genes. The structural characteristics of the <i>R.mariesii</i>cp genome was similar to other angiosperms. Leucine was the most representative amino acid, while cysteine was the lowest representative. Totally, 30 codons showed obvious codon usage bias, and most were A/U-ending codons. Six highly variable regions were observed, such as <i>trnK-pafI</i> and <i>atpE-rpoB</i>, which could serve as potential markers for future barcoding and phylogenetic research of <i>R.mariesii</i> species. Coding regions were more conserved than non-coding regions. Expansion and contraction in the IR region might be the main length variation in <i>R.mariesii</i> and related Ericaeae species. Maximum-likelihood (ML) phylogenetic analysis revealed that <i>R.mariesii</i> was relatively closed to the <i>R.simsii</i> Planchon, 1853 and <i>R.pulchrum</i> Sweet,1831. This research will supply rich genetic resource for <i>R.mariesii</i> and related species of the Ericaeae.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"17 ","pages":"163-180"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10464601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10482830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First karyotype description of the species of <i>Adenomera</i> Steindachner, 1867 (Anura, Leptodactylidae) in the \" <i>thomei</i>\" clade.","authors":"Ramon Costa Dominato, Guilherme Costa de Oliveira, Carla Santana Cassini, Victor Goyannes Dill Orrico, Cléa Dos Santos Ferreira Mariano, Janisete Gomes Silva","doi":"10.3897/compcytogen.v16.i3.82641","DOIUrl":"10.3897/compcytogen.v16.i3.82641","url":null,"abstract":"<p><p>The genus <i>Adenomera</i> Steindachner, 1867 currently comprises 29 nominal species, some of which are suggested to be cryptic species complexes. The present study was carried out with specimens of the \"<i>thomei</i>\" clade that encompasses three taxa distributed in the Atlantic Forest biome: <i>Adenomerathomei</i> Almeida et Angulo, 2006, <i>Adenomera</i> sp. L., and <i>Adenomera</i> sp. M. We used classical cytogenetics to describe the diploid number and karyomorphology of these three species collected in two different locations in the state of Bahia, Brazil. Our results revealed the diploid number 2n = 24 (FN = 34) with two pairs of metacentric chromosomes (pairs 1 and 5), three pairs of submetacentric chromosomes (pairs 2, 3, and 4), and seven pairs of telocentric chromosomes (pairs 6, 7, 8, 9, 10, 11, and 12). Further morphological, bioacoustic, and cytogenetic data (C-banding and AgNor) are needed to better delineate the lineages within the \"<i>thomei</i>\" clade.</p>","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 3","pages":"151-159"},"PeriodicalIF":1.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10689447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}