Current Proteomics最新文献

{"title":"Investigating PI3P Binding with Plasmodium Falciparum HSP70 Proteins","authors":"Vipul Upadhyay, Satinder Kaur, Rachna Hora, Prakash Chandra Mishra","doi":"10.2174/0115701646297476240408042556","DOIUrl":"https://doi.org/10.2174/0115701646297476240408042556","url":null,"abstract":"aims: Investigating the binding of phosphatidylinositol 3 phosphate (PI3P) with Plasmodium falciparum heat shock protein 70 homologs background: The 70 kDa Plasmodium falciparum (Pf) heat shock proteins (PfHSP70s) are an important class of molecules that are critically involved in parasite survival through periods of stress. Interaction between a crucial lipid modulatorPI3P and the C terminal lid domain of cytosolic PfHSP70-1, stabilizes the parasite digestive vacuole (DV) to facilitate hemoglobin trafficking and breakdown in turn impacting parasite survival. PfHSP70 homologs and PI3P are amply expressed together in various subcellular compartments of the parasite providing these with an opportunity to interact and affect biological processes. objective: The objectives of this study included identification of the PI3P binding pockets on PfHSP70s, understanding the binding affinity of each homolog for PI3P and identifying residues involved in different types of interactions. method: We have analyzed PI3P binding of all four PfHSP70s by using bioinformatics tools like sequence analysis, molecular docking and LigPlot+ analysis. result: Our results show that differently localized PfHSP70 homologs bind PI3P with variable affinity. The mitochondrial and ER localized PfHSP70s bind more strongly with PI3P than their cytosolic counterpart PfHSP70-1. The PI3P binding region on all PfHSP70 homologs lies at the interface of helices 1 and 3 of their substrate binding domain . Analysis of these results has also helped to pinpoint specific residues on PfHSP70s that may be engaged in these interactions. conclusion: PI3P preferentially binds to a particular pocket on PfHSP70 homologs. Different PfHSP70s bind to PI3P with variable binding affinity. Specific residues of PfHSP70 homologs involved in these interactions have been identified. other: The present study may therefore form the basis for designing interventions that hinder PfHSP70-PI3P interaction and influence parasite survival.","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":"303 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a Novel Multi-Epitope Peptide as a Potential Serodiagnosis Marker for the Diagnosis of Acinetobacter baumannii: An In silico Approach","authors":"Maryam Rezaee, Mohsen Mohammadi, Amir Savardashtaki, Mohammad Reza Rahbar, Navid Nezafat","doi":"10.2174/0115701646297689240325062145","DOIUrl":"https://doi.org/10.2174/0115701646297689240325062145","url":null,"abstract":"Background: Acinetobacter baumannii is an opportunistic pathogen that causes many infections, including nosocomial infections; this bacterium has a high mortality rate among other bacteria. A. baumannii has an elastic genome that changes rapidly when exposed to harsh environmental conditions, leading to widespread bacterial resistance to various disinfectants and antibiotics. The high ability of bacteria to bind to all surfaces and survive in different conditions has caused the spread of bacteria in various environments. Rapid detection is very important in preventing the spread and even treatment of the infection. Methods: Currently, the Polymerase Chain Reaction (PCR) method is the only effective method used for diagnosis, which has some pros and cons. Results and Conclusion: This study aimed to design a new recombinant multi-epitope protein from Acinetobacter baumannii that can be used in ELISA for rapid diagnosis. The unique feature of this study from others is the use of patient serum for antibody monitoring.","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":"50 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140585015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic Analysis of the Molecular Mechanisms of Chlorpromazine Inhibiting Migration of Oral Squamous Cell Carcinoma","authors":"Nannan Zhang, Junzhi Liu, Qiuping Dong, Chen Liu, Xinyu Liang, Peiyuan Tang, Zheng Liang","doi":"10.2174/0115701646291510240212091951","DOIUrl":"https://doi.org/10.2174/0115701646291510240212091951","url":null,"abstract":"Background: Oral squamous cell carcinoma (OSCC) is a common malignant tumor of the head and neck region known for its high metastatic and invasive potential. Chlorpromazine (CPZ) has been shown to inhibit the growth of oral cancer cells. However, the effects of CPZ on OSCC migration and its underlying molecular mechanisms remain unclear. Objective: We aimed to identify global protein changes and potential core proteins involved in CPZ-mediated inhibition of migration in SCC-15 cells using proteomics. Methods: We assessed the effect of CPZ on SCC-15 using CCK-8 assays and wound healing experiments. Next, we performed LC-MS-based proteomic analysis to identify protein alterations in SCC-15 cells treated with CPZ at different times. Differential expression proteins (DEPs) were identified and subjected to bioinformatics analysis using GO, KEGG, and PPI tools. Key candidate proteins were selected and validated using the TCGA-HNSCC database and molecular docking. Results: It was found that 20μm of CPZ had no effect on cell proliferation, but inhibited cell migration. A total of 4748 proteins were identified by Proteomics, among which 56 DEPs were identified, including 34 upregulated proteins and 22 downregulated proteins. Three proteins (RPF2, ACTB, and TGFBI) were identified as key candidate proteins associated with cell adhesion and migration in oral cancer cells. Conclusion: CPZ may affect the expression of RPF2, ACTB, and TGFBI proteins and change the extracellular matrix and cell adhesion function, thus inhibiting the migration of SCC-15 cells. The results of this study provide a robust basis for further research on the molecular mechanism of CPZ to inhibit the migration of OSCC.","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":"43 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140008576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoprotective Potential of Adenylosuccinate Synthetase Protein (PurA) in Streptococcus equi ssp. zooepidemicus Infections","authors":"Xiaoling Zhang, Sihuan Chen, Shuji Gao, Weiping Yang, Yuxin Wang, Yang Wang, Li Yi","doi":"10.2174/0115701646284439240218063821","DOIUrl":"https://doi.org/10.2174/0115701646284439240218063821","url":null,"abstract":" Background: Streptococcus equi ssp. zooepidemicus(SEZ) is one important pathogen. There are still sporadic outbreaks in China, northern United States and the Netherlands. Adenylosuccinate synthetase PurA, a newly discovered protein in prior research, requires further assessment of its protective effectiveness. Methods: In this study, we focused on the expression of recombinant PurA from SEZ ATCC 35246. We evaluated the immunoreactivity of this recombinant protein using convalescent minipig sera. Additionally, we conducted experiments in mice to assess its immunogenic properties. Results: Our findings revealed that the recombinant PurA triggered a substantial antibody response in mice, resulting in an 80% protection rate against SEZ infection. Notably, mice immunized with PurA exhibited significantly reduced bacterial colonization in all organs compared to the PBS control group. Furthermore, the levels of IL-6, IL-8, IL-1β, and TNF-α in mouse serum were significantly elevated in the PurA-immunized group compared to the control group. Hyperimmune sera targeting PurA effectively eliminated SEZ in bactericidal tests. Remarkably, antibodies against PurA demonstrated a significant inhibitory effect on developing SEZ biofilm. Conclusion: Immunization with PurA elicited robust humoral and cellular immune responses in mice. These promising results suggest the potential utility of PurA in developing SEZ vaccine immunogens, providing a valuable avenue for further research into SEZ infection prevention and control.","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":"20 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141165687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of miR-135a-5p Promotes Mitophagy by Regulating FoxO1/PINK1/Parkin Signaling in Hepatoma Cells Exposed to Oxidative Stress","authors":"Wang Zhenchang, Zhang Wenf, Wu Shanshan, Yang Lei","doi":"10.2174/0115701646258315231102070151","DOIUrl":"https://doi.org/10.2174/0115701646258315231102070151","url":null,"abstract":"Introduction: Excessive oxidative stress is always associated with hepatic disease, including hepatitis, liver fibrosis, and hepatocellular carcinoma (HCC). Despite this, the intricate molecular processes driving hepatocyte apoptosis due to oxidative stress remain incompletely comprehended. Aim: Consequently, we aimed to explore the role of miR-135a-5p in hepatoma cells (HepG2/3B). Methods: The assessment of protein expression was conducted through western blotting. Furthermore, miR-135a-5p expression was evaluated through RT-qPCR, and apoptosis detection was performed using a flow cytometry assay. Result: The findings suggest a connection between miR-135a-5p and mitochondrial-driven apoptosis through caspase signaling pathways. Furthermore, miR-135a-5p suppression inhibited the apoptotic response triggered by H2O2, reactive oxygen species (ROS) generation, as well as the decrease in mitochondrial membrane potential. Conclusion: Additionally, miR-135a-5p knockdown promoted mitophagy by regulating FoxO1/PINK1/Parkin signaling via targeting FoxO1. To conclude, our study implied that miR135a-5p might function as a probable regulator that protects cells against oxidative stress.","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":"23 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138572423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PCM1: A Potential Prognostic Biomarker Correlated with Immune Infiltration in Lung Adenocarcinoma","authors":"Zhihua Guo, Jinghao Liang, Xin Zhang, Qing Ai, Zixian Xie, Haonan Zhao, Fayuan Wu, Zhaofeng Tan, Weiqiang Yin, Linghua Ji","doi":"10.2174/0115701646270898231123065507","DOIUrl":"https://doi.org/10.2174/0115701646270898231123065507","url":null,"abstract":"Background: Recent studies have validated the role of Pericentriolar Material 1 (PCM1) in several malignant tumour cell lines, but its specific biological function in lung adenocarcinoma (LUAD) remains unclear. Objective: To address this gap, this study analyzed 411 LUAD and control samples to evaluate the prognostic value of PCM1 using Cox regression analysis. objective: To address this gap, this study analyzed 411 LUAD and control samples to evaluate the prognostic value of PCM1 using Cox regression analysis. Methods: Multiple genes co-expressed with PCM1 were also analyzed to investigate the biological processes and roles involved in PCM1. An endogenous competitive network with PCM1 as the key gene was constructed to uncover its regulatory and competitive relationships in LUAD. The study further explored the immunological characteristics of PCM1 in different expression groups based on immune infiltration analysis. Results: These findings indicated that higher PCM1 expression levels were associated with better survival prognoses, possibly due to its antagonistic effects on RHOC. Immunological infiltration analysis revealed a significant correlation between PCM1 and various immune cell infiltration levels, including CD4+ T cells, naïve B cells, M2 macrophages, and mast cells. However, there was no significant relationship between PCM1 and MSI, TMB, or stemness, although it was positively correlated with m6A genes. Patients with lower PCM1 expression responded better to CTLA-4 therapy. The study also estimated that some chemotherapeutic and targeted agents might be effective in treating patients with high PCM1 levels. PCM1 was mainly expressed in the cytoplasmic and membranous structures. Conclusion: PCM1 shows potential as a prognostic biomarker for LUAD due to its strong correlation with immune cell infiltration and its ability to enhance anticancer treatment sensitivity. other: No.","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":"159 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138572021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a New Hypotensive Peptide from the Venom of Snake bothrops jararaca (Bj)","authors":"Ajoy Basak, Euridice Carmona, Sanjukta Basak, Felicia Au, Rosa Anna Maria Barbarulo Borgheresi","doi":"10.2174/0115701646254996231130050528","DOIUrl":"https://doi.org/10.2174/0115701646254996231130050528","url":null,"abstract":"Background: Snake venom has become a key source of many bioactive peptides, enzymes, and toxins associated with blood coagulation and neuronal toxicity. In the past, a number of bradykinin potentiating peptides have been isolated from snake venom that display hypotensive activity due to their inhibitory activity towards Angiotensin-Converting Enzyme (ACE). Significant interest has developed to isolate, characterize, and subsequently design peptide analogs as potent ACE-inhibitors which may find therapeutic applications for the treatment of hypertension and associated diseases. Aim: The aim of this study is to search for new bioactive peptide/s in the venom of the snake Bothrops Jararaca (Bj). Objective: The objective is to isolate and characterize new hypotensive peptides from BJ venom. Methodology: We examined the venom of Bj which is known to host a range of bioactive peptides. We have isolated a new peptide (BJ-1) which displayed in vitro potent hypotensive activity. The peptide was purified via Sephadex G25 column chromatography and RP-HPLC. It was characterized by mass spectrometry, amino acid analysis, N-terminal sequencing, and chemical synthesis. Result: The peptide was identified as an octa-decapeptide with an amino acid sequence as DCPSDWSSYEGHCYKPFS where the two Cys residues are likely present in a free state, although they can form an internal S-S bond upon oxidation. It was fully confirmed by comparing it with synthetic peptides prepared by solid phase chemistry. Both have the same molecular mass (2,108 Da) and identical bioactivity. Furthermore, we rationalize that BJ-1 may be derived from precursor protein “Coagulation factor IX/factor X binding protein (CF-IX/X-BP)” by proteolytic cleavage at the Nterminus of its A-chain within the sequence KPFS18ↆE 19PKN. This cleavage site contains the recognition motif of enzyme PCSK8 (Proprotein Convertase Subtilisin Kexin8) also known as Subtilisin Kexin Isozyme 1 (SKI-1) or Site 1 Protease (S1P). Despite this observation, using a synthetic peptide encompassing the proposed cleavage site and recombinant PCSK8 enzyme, we found that the enzyme responsible for the generation of BJ-1 is not PCSK8. Further studies will be needed to identify the associated enzyme and fully characterize the pharmacological and biological properties of the peptide. Conclusion: Our study revealed the presence of a novel hypotensive octa-decapeptide in the venom of the snake Bothrops jararaca. It is likely derived from the A-chain of protein CF-IX/X-BP via proteolytic cleavage at the N-terminus by a protease yet to be characterized","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":"23 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein Profiling of Hirudo orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy","authors":"Leili Amani, Mehran Mirabzadeh Ardakani, Nasrin Motamed, Masuomeh Malek, Marzieh Dehghan Shasaltaneh","doi":"10.2174/0115701646257568231130053824","DOIUrl":"https://doi.org/10.2174/0115701646257568231130053824","url":null,"abstract":"Leech therapy has been used for centuries as a recommended approach to cure several diseases, such as; psoriasis, arthrosclerosis, urinary tract diseases, and wound healing. The present study aimed to analyze the number, quantity, and distribution differences of medicinal leech (Hirudo orientalis) proteins throughout various seasons and in laboratory conditions as well. Protein profiling of salivary gland secretion from leech was studied by SDS-PAGE and 2D Electrophoresis on the proteins with the molecular weight range of 5 - 250 KDa in the lyophilized salivary gland secretion (SGS) during the seasons of summer and winter, and also in the laboratory conditions. Our results indicated differences in the number and quality of leech saliva proteins in different seasons. We observed a higher number of proteins in summer than in winter. These results demonstrated the presence of Calin and Manillase in summer and Hyaluronidase and Collagenase in winter. This study could help us in choosing the best and most favorable conditions for using H. orientalis proteins for the treatment of different diseases","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":"128 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of C-terminal Truncated Beta-amyloid Peptides with Human Serum Albumin","authors":"Diba Aslani Firozabadi, Mohammad Reza Bozorgmehr, S. Ali Beiramabadi, Sharareh Mohseni","doi":"10.2174/0115701646243074231113071548","DOIUrl":"https://doi.org/10.2174/0115701646243074231113071548","url":null,"abstract":"Background: The formation of plaque from protein fibrils is the major source of diseases, such as Alzheimer's and Prion diseases. Amyloid beta (Aβ) is a peptide with different lengths, which is one of the main components of the plaque in the brain of people with Alzheimer's. Of the amyloid beta of various lengths in the brain cells plaque, beta-amyloid with 40 amino acids (Aβ1- 40) is more abundant than the rest. Aβ monomers are in a dynamic equilibrium of various conformations with beta sheets that aggregate as oligomers or larger structures. The misfolding of betaamyloid peptide is involved in its accumulation. On the other hand, various species that exist in the cell environment can affect the structure of beta-amyloid peptides. background: The accumulation of beta-amyloid peptide is one of the effective mechanisms in creating amyloid plaques in nerve cells. These plaques are responsible for causing Alzheimer's disease Aims: This study aimed to study the interaction of truncated forms of beta-amyloid peptide with human albumin serum protein. objective: Interaction of beta-amyloid peptide with other proteins is effective in causing Alzheimer's disease. These include interactions between beta-amyloid and cell surface proteins such as prions and extracellular proteins such as clusterins and human serum albumin (HSA). Because HSA concentrations are higher than other proteins, more than half of the interaction of beta-amyloid with proteins is related to interaction with this protein. Interaction of HSA with beta-amyloid somewhat reduces the aggregation of beta amyloid. However, due to the diversity of beta-amyloid peptides with different lengths, the mechanism of their interaction with HSA has not been well understood. In this work, the interaction of C-terminal truncated beta-amyloid peptides with HSA has been investigated. Objective: Interaction of beta-amyloid peptide with other proteins is effective in causing Alzheimer's disease. These include interactions between beta-amyloid and cell surface proteins, such as prions and extracellular proteins, such as clusterins and human serum albumin (HSA). As HSA concentrations are higher than other proteins, more than half of the interaction of beta-amyloid with proteins is related to interaction with this protein. Interaction of HSA with beta-amyloid reduces the aggregation of beta-amyloid. However, due to the diversity of beta-amyloid peptides with different lengths, the mechanism of their interaction with HSA has not been well understood. In this work, the interaction of C-terminal truncated beta-amyloid peptides with HSA has been investigated. Method: The C-terminal truncated forms of beta-amyloid peptides, Aβ1 − 26, Aβ1 − 30, and Aβ1 − 36 and Aβ1 − 40, were designed in silico. Docking between these truncated peptides was performed with serum albumin. A molecular dynamics simulation of the interaction of designed peptides with serum albumin was also performed. Results and Discussion","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":" 10","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing Multi-epitope Antigen Construct from Immunodominant Proteins for Serological Diagnosis of Chlamydia trachomatis: An In Silico Approach","authors":"Farideh Ghalamfarsa, Amir Savardashtaki, Cambyz Irajie, Amir Emami, Navid Nezafat, Younes Ghasemi","doi":"10.2174/0115701646244648231014153217","DOIUrl":"https://doi.org/10.2174/0115701646244648231014153217","url":null,"abstract":"Background: Chlamydiasis is a widespread bacterial infection in the world. Serological tests are expensive, and in addition, intrinsic antigens can cause cross-reactions and make the diagnosis process difficult. Multi-epitope protein antigens are novel and potential diagnostic markers that have the capability of more accurate and cheaper diagnosis. Therefore, in this study, the main goal is to design a new protein vaccine, including multiple epitopes of B cells with dominant immunity from three proteins named MOMP, ompA and Pgp3D from C. trachomatis Methods: The amino acid sequences were obtained from the UniProt database. The areas with the highest antigenicity were identified using the EMBOSS server. Linear B cell epitopes were determined using BCPRED, ABCpred, and Bepipred servers. Epitopes with the highest antigenicity were connected using the EAAAK linker. Results: Two epitopes from MOMP, two from ompA, and one from Pgp3D were selected. These epitopes were connected to each other with the EAAAK linker. Three residues (0.592), 16 residues (0.76), 36 residues (0.578), and 37 residues (0.734) were obtained from the prediction of the spatial structure of the B cell multiple epitopes designed with ElliPro. Model 1 of RaptorX was selected as the best structure. In this model, the ERRAT quality, ProSA-web z-score, and Verify3D were 83.1169, - 5.17 and 84.62% with PASS score, respectively. Moreover, the Ramachandran plot showed that 86.093% of the amino acid residues were located in the favored region. To achieve the highest level of protein expression, the designed multi-epitope reverse-translated with the Genscript server and was cloned in E. coli. The highest level of expression was achieved, and a CAI score of 0.91 was reported. The gene GC content was 51.98%, and the contribution of low-frequency codons was 0%. Conclusion: The results confirmed that the designed construct could identify C. trachomatis with high sensitivity and specificity in serum samples of patients with chlamydiasis. However, further experimental studies are needed for final confirmation.","PeriodicalId":50601,"journal":{"name":"Current Proteomics","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136316945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}