不同分析方法评价蓖麻蛋白与槲皮素的相互作用

IF 0.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Current Proteomics Pub Date : 2023-07-17 DOI:10.2174/1570164620666230717114018

L. Hernández-Ochoa, M. Martínez-Ceniceros, L. O. Nevarez-Prado, David Neder‐Suárez, F. Sandoval-Salas, L. Rodríguez-Valdez, L. Landeros-Martínez, Karla Bernal-Alvarado

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引用次数: 0

摘要

蓖麻毒素是已知毒性最强的蛋白质。它是核糖体失活蛋白(RIPs, type 2)的一部分，在他的研究中产生了重要意义;用酚类化合物来解毒这种蛋白质是可能的;然而，有必要了解这种解毒是如何发生的。采用电泳、紫外可见光谱和高效液相色谱分析蓖麻毒素蛋白与槲皮素黄酮醇的解毒过程。对样品的上清液和沉淀物进行紫外可见分析;采用单因素方差分析(ANOVA)和Tukey检验对提取结果进行分析，总蛋白含量显著水平为0.05.34.9 μg / mL，蓖麻毒素含量显著水平为4.2 μg / mL。共进行了8次相互作用，均出现沉淀，通过电泳技术观察到蛋白质对应的条带减少;用高效液相色谱法分析这些结果，观察到色谱峰面积的大小减小。在这项研究中获得的结果表明，蛋白质的团聚，产生沉淀，可能有利于蛋白质的失活作为一个解毒过程。

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Evaluation of the interaction between ricin protein and quercetin using different analytical methods

Ricin is the most toxic protein known. It is part of the ribosome-inactivating proteins, RIPs, type 2, which has generated importance in his research; it is possible to detoxify this protein with phenolic compounds; however, it is essential to understand how this detoxification occurs. To analyze using electrophoresis, UV-visible spectroscopy, and high-performance liquid chromatography (HPLC) the protein ricin with the flavonol quercetin, understanding the detoxification process. The UV-visible analysis was performed on both the supernatant and the precipitate of the samples; these results were analyzed using one-factor analysis of variance (ANOVA) and a Tukey test with a significance level of 0.05. 34.9 μg / mL of total protein and 4.2 μg / mL of ricin were obtained in the extraction method. Eight interactions were carried out, and all presented precipitation, observing through the electrophoresis technique a decrease in the bands corresponding to the protein; these results were analyzed with HPLC observing a decrease in the size of the area of the peaks in the chromatograms. The results obtained in this study suggest an agglomeration of the protein, generating a precipitate that could benefit the protein's inactivation as a detoxification process.

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来源期刊

Current Proteomics BIOCHEMICAL RESEARCH METHODS-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed in-depth/mini review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry. Current Proteomics publishes in-depth/mini review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, software, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to: Protein separation and characterization techniques 2-D gel electrophoresis and image analysis Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching Determination of co-translational and post- translational modification of proteins Protein/peptide microarrays Biomolecular interaction analysis Analysis of protein complexes Yeast two-hybrid projects Protein-protein interaction (protein interactome) pathways and cell signaling networks Systems biology Proteome informatics (bioinformatics) Knowledge integration and management tools High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography) High-throughput computational methods for protein 3-D structure as well as function determination Robotics, nanotechnology, and microfluidics.