Effects of Chronic Cold Exposure on Proteomics of Lung Tissue in Mice

IF 0.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Current Proteomics Pub Date : 2023-10-26 DOI:10.2174/0115701646245422231013072302

Moyou Li, Ying Liu, Xiaoye Tian, Zhuojun Wang, Feng Cheng, Xiao Han, Zheyuan Chen, Ruihang Ma, Hongxu Jin

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引用次数: 0

Abstract

Background: Cold exposure can induce inflammation-related injury in lung tissue, but the exact mechanism is still unclear. Objective: The study aimed to clarify the proteomic characteristics of lung tissue under cold exposure. Methods: Forty mice were randomly equally divided into a control group and a model group. The model group was exposed to - 20 °C for two weeks (4 hours per day), while the control group was maintained at 22 ± 2 °C. H&E staining and ELISA were used to verify the injury of lung tissue. Furthermore, a quantitative analysis of the overall proteome in the lung of mice exposed to cold stress was conducted by using LC-MS/MS. 15 differentially expressed proteins were selected for PRM validation. Results: According to our results, cold exposure induced lung injury, and the expressions of 151 proteins were upregulated and those of 95 proteins were downregulated. Bioinformatics analysis showed that differentially expressed proteins were associated with tricarboxylic acid cycle, fat metabolism, glycolysis, and oxidative phosphorylation. The expression of gabra2, Klkb1, and complement-related proteins was significantly upregulated. The results of PRM validation were consistent with those of proteomics. Conclusion: We found changes in glycolysis, gabra2, Klkb1, and the complement system in the lung tissue of cold-stressed mice, which may play an important role in cold stress-induced lung injury

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慢性冷暴露对小鼠肺组织蛋白质组学的影响

背景:冷暴露可诱导肺组织炎症相关损伤，但确切机制尚不清楚。目的:研究低温下肺组织的蛋白质组学特征。方法:将40只小鼠随机分为对照组和模型组。模型组在- 20℃下连续2周(每天4小时)，对照组维持在22±2℃。采用H&E染色和ELISA法验证肺组织损伤情况。此外，我们还利用LC-MS/MS对冷应激小鼠肺部的整体蛋白质组进行了定量分析。选择15个差异表达蛋白进行PRM验证。结果:低温暴露诱导肺损伤，151个蛋白表达上调，95个蛋白表达下调。生物信息学分析显示，差异表达蛋白与三羧酸循环、脂肪代谢、糖酵解和氧化磷酸化有关。gabra2、Klkb1和补体相关蛋白的表达显著上调。PRM验证结果与蛋白质组学验证结果一致。结论:我们发现冷应激小鼠肺组织中糖酵解、gabra2、Klkb1和补体系统的变化可能在冷应激诱导的肺损伤中起重要作用

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来源期刊

Current Proteomics BIOCHEMICAL RESEARCH METHODS-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed in-depth/mini review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry. Current Proteomics publishes in-depth/mini review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, software, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to: Protein separation and characterization techniques 2-D gel electrophoresis and image analysis Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching Determination of co-translational and post- translational modification of proteins Protein/peptide microarrays Biomolecular interaction analysis Analysis of protein complexes Yeast two-hybrid projects Protein-protein interaction (protein interactome) pathways and cell signaling networks Systems biology Proteome informatics (bioinformatics) Knowledge integration and management tools High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography) High-throughput computational methods for protein 3-D structure as well as function determination Robotics, nanotechnology, and microfluidics.