Development Genes and Evolution最新文献_第2页

Molecular evolution of transcription factors AF4/FMR2 family member (AFF) gene family and the role of lamprey AFF3 in cell proliferation. 转录因子 AF4/FMR2 家族成员（AFF）基因家族的分子进化及灯鱼 AFF3 在细胞增殖中的作用。

IF 0.8 3区生物学

Development Genes and Evolution Pub Date : 2024-06-01 Epub Date: 2024-05-11 DOI: 10.1007/s00427-024-00717-1

Difan Sun, Xinyu Du, Peng Su

{"title":"Molecular evolution of transcription factors AF4/FMR2 family member (AFF) gene family and the role of lamprey AFF3 in cell proliferation.","authors":"Difan Sun, Xinyu Du, Peng Su","doi":"10.1007/s00427-024-00717-1","DOIUrl":"10.1007/s00427-024-00717-1","url":null,"abstract":"AF4/FMR2 family member (AFF) proteins are a group of transcriptional regulators that can regulate gene transcription and play an important role in cellular physiological processes such as proliferation and differentiation. The transcriptome data of the lamprey spinal cord injury were analyzed in previous research. We then identified a hub gene, Lr-AFF3, from this dataset. Phylogenetic tree analysis determined the evolutionary relationships of the AFF gene family across different species. In addition, analysis of motifs, domains, and 3D structures further confirmed the conservatism of the AFF gene family. In particular, the gene structure of the AFF3 gene was not conserved, possibly because of intron insertion. It was also found that the neighboring genes of the Lr-AFF3 gene had a higher diversity than that in jawed vertebrates through synteny analysis. The results of the MTT and EdU experiments showed that the C-terminal homology domain (CHD) and N-terminal homology domain (NHD) of Lr-AFF3 promoted cell proliferation. In summary, our research will not only provide new insights into the origin and evolution of the AFF gene family in different species, but also provide new clues for the functions of Lr_AFF3.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":" ","pages":"45-53"},"PeriodicalIF":0.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140909545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From bristle to brain: embryonic development of topographic projections from basiconic sensilla in the antennal nervous system of the locust Schistocerca gregaria 从鬃毛到大脑：蝗虫触角神经系统中基本感觉器的地形投射的胚胎发育过程

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2024-05-01 DOI: 10.1007/s00427-024-00716-2

George Boyan, Erica Ehrhardt

{"title":"From bristle to brain: embryonic development of topographic projections from basiconic sensilla in the antennal nervous system of the locust Schistocerca gregaria","authors":"George Boyan, Erica Ehrhardt","doi":"10.1007/s00427-024-00716-2","DOIUrl":"https://doi.org/10.1007/s00427-024-00716-2","url":null,"abstract":"The antennal flagellum of the locust S. gregaria is an articulated structure bearing a spectrum of sensilla that responds to sensory stimuli. In this study, we focus on the basiconic-type bristles as a model for sensory system development in the antenna. At the end of embryogenesis, these bristles are found at fixed locations and then on only the most distal six articulations of the antenna. They are innervated by a dendrite from a sensory cell cluster in the underlying epithelium, with each cluster directing fused axons topographically to an antennal tract running to the brain. We employ confocal imaging and immunolabeling to (a) identify mitotically active sense organ precursors for sensory cell clusters in the most distal annuli of the early embryonic antenna; (b) observe the subsequent spatial appearance of their neuronal progeny; and (c) map the spatial and temporal organization of axon projections from such clusters into the antennal tracts. We show that early in embryogenesis, proliferative precursors are localized circumferentially within discrete epithelial domains of the flagellum. Progeny first appear distally at the antennal tip and then sequentially in a proximal direction so that sensory neuron populations are distributed in an age-dependent manner along the antenna. Autotracing reveals that axon fasciculation with a tract is also sequential and reflects the location and age of the cell cluster along the most distal annuli. Cell cluster location and bristle location are therefore represented topographically and temporally within the axon profile of the tract and its projection to the brain.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"39 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140831416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of the Dmrt1B gene in the oriental river prawn, Macrobrachium nipponense 东方对虾 Dmrt1B 基因的鉴定和特征描述

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2024-04-15 DOI: 10.1007/s00427-024-00715-3

Gang Jiang, Yucai Xue, Arifuzzaman Arifuzzaman, Xuxiong Huang

{"title":"Identification and characterization of the Dmrt1B gene in the oriental river prawn, Macrobrachium nipponense","authors":"Gang Jiang, Yucai Xue, Arifuzzaman Arifuzzaman, Xuxiong Huang","doi":"10.1007/s00427-024-00715-3","DOIUrl":"https://doi.org/10.1007/s00427-024-00715-3","url":null,"abstract":"Dmrt (doublesex and mab-3 related transcription factor) is a protein family of transcription factors implicated in sexual regulation. Dmrt proteins are widely conserved and known for their involvement in sex determination and differentiation across species, from invertebrates to humans. In this study, we identified a novel gene with a DM (doublesex/Mab-3)-domain gene in the river prawn, Macrobrachium nipponense, which we named MniDmrt1B due to its similarities and close phylogenetic relationship with Dmrt1B in Macrobrachium rosenbergii. Through amino acid alignments and structural predictions, we observed conservation and identified putative active sites within the DM domain. qRT-PCR analysis revealed that MniDmrt1B exhibited high expression levels in the testis, with consistently higher expression in males compared to females during development. Additionally, similar to other sex-regulated genes, the MniDmrt1B gene exhibited high expression levels during the sex differentiation–sensitive periods in M. nipponense. These results strongly indicated that MniDmrt1B probably plays an important role in testis development and sex differentiation in M. nipponense.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"252 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140576336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of 20 SCPP genes during tooth and bone mineralization in Senegal bichir. 塞内加尔比奇犬牙齿和骨骼矿化过程中 20 个 SCPP 基因的表达。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-12-01 Epub Date: 2023-07-06 DOI: 10.1007/s00427-023-00706-w

S Delgado, M A Fernandez-Trujillo, G Houée, J Silvent, X Liu, E Corre, J Y Sire

{"title":"Expression of 20 SCPP genes during tooth and bone mineralization in Senegal bichir.","authors":"S Delgado, M A Fernandez-Trujillo, G Houée, J Silvent, X Liu, E Corre, J Y Sire","doi":"10.1007/s00427-023-00706-w","DOIUrl":"10.1007/s00427-023-00706-w","url":null,"abstract":"The African bichir (Polypterus senegalus) is a living representative of Polypteriformes. P. senegalus possesses teeth composed of dentin covered by an enameloid cap and a layer of collar enamel on the tooth shaft, as in lepisosteids. A thin layer of enamel matrix can also be found covering the cap enameloid after its maturation and during the collar enamel formation. Teleosts fish do not possess enamel; teeth are protected by cap and collar enameloid, and inversely in sarcopterygians, where teeth are only covered by enamel, with the exception of the cap enameloid in teeth of larval urodeles. The presence of enameloid and enamel in the teeth of the same organism is an opportunity to solve the evolutionary history of the presence of enamel/enameloid in basal actinopterygians. In silico analyses of the jaw transcriptome of a juvenile bichir provided twenty SCPP transcripts. They included enamel, dentin, and bone-specific SCPPs known in sarcopterygians and several actinopterygian-specific SCPPs. The expression of these 20 genes was investigated by in situ hybridizations on jaw sections during tooth and dentary bone formation. A spatiotemporal expression patterns were established and compared with previous studies of SCPP gene expression during enamel/enameloid and bone formation. Similarities and differences were highlighted, and several SCPP transcripts were found specifically expressed during tooth or bone formation suggesting either conserved or new functions of these SCPPs.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":" ","pages":"91-106"},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9758904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of posterior Hox genes and opisthosomal appendage development in a mygalomorph spider. 髓蛛后部 Hox 基因的表达和乳突附肢的发育

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-12-01 Epub Date: 2023-07-27 DOI: 10.1007/s00427-023-00707-9

Ralf Janssen, Matthias Pechmann

{"title":"Expression of posterior Hox genes and opisthosomal appendage development in a mygalomorph spider.","authors":"Ralf Janssen, Matthias Pechmann","doi":"10.1007/s00427-023-00707-9","DOIUrl":"10.1007/s00427-023-00707-9","url":null,"abstract":"Spiders represent an evolutionary successful group of chelicerate arthropods. The body of spiders is subdivided into two regions (tagmata). The anterior tagma, the prosoma, bears the head appendages and four pairs of walking legs. The segments of the posterior tagma, the opisthosoma, either lost their appendages during the course of evolution or their appendages were substantially modified to fulfill new tasks such as reproduction, gas exchange, and silk production. Previous work has shown that the homeotic Hox genes are involved in shaping the posterior appendages of spiders. In this paper, we investigate the expression of the posterior Hox genes in a tarantula that possesses some key differences of posterior appendages compared to true spiders, such as the lack of the anterior pair of spinnerets and a second set of book lungs instead of trachea. Based on the observed differences in posterior Hox gene expression in true spiders and tarantulas, we argue that subtle changes in the Hox gene expression of the Hox genes abdA and AbdB are possibly responsible for at least some of the morphological differences seen in true spiders versus tarantulas.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":" ","pages":"107-121"},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10040450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genomic landscape of mammal domestication might be orchestrated by selected transcription factors regulating brain and craniofacial development. 哺乳动物驯化的基因组图谱可能是由调节大脑和颅面发育的特定转录因子精心策划的。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-12-01 Epub Date: 2023-08-08 DOI: 10.1007/s00427-023-00709-7

Antonio Benítez-Burraco, Juan Uriagereka, Serge Nataf

{"title":"The genomic landscape of mammal domestication might be orchestrated by selected transcription factors regulating brain and craniofacial development.","authors":"Antonio Benítez-Burraco, Juan Uriagereka, Serge Nataf","doi":"10.1007/s00427-023-00709-7","DOIUrl":"10.1007/s00427-023-00709-7","url":null,"abstract":"Domestication transforms once wild animals into tamed animals that can be then exploited by humans. The process entails modifications in the body, cognition, and behavior that are essentially driven by differences in gene expression patterns. Although genetic and epigenetic mechanisms were shown to underlie such differences, less is known about the role exerted by trans-regulatory molecules, notably transcription factors (TFs) in domestication. In this paper, we conducted extensive in silico analyses aimed to clarify the TF landscape of mammal domestication. We first searched the literature, so as to establish a large list of genes selected with domestication in mammals. From this list, we selected genes experimentally demonstrated to exhibit TF functions. We also considered TFs displaying a statistically significant number of targets among the entire list of (domestication) selected genes. This workflow allowed us to identify 5 candidate TFs (SOX2, KLF4, MITF, NR3C1, NR3C2) that were further assessed in terms of biochemical and functional properties. We found that such TFs-of-interest related to mammal domestication are all significantly involved in the development of the brain and the craniofacial region, as well as the immune response and lipid metabolism. A ranking strategy, essentially based on a survey of protein-protein interactions datasets, allowed us to identify SOX2 as the main candidate TF involved in domestication-associated evolutionary changes. These findings should help to clarify the molecular mechanics of domestication and are of interest for future studies aimed to understand the behavioral and cognitive changes associated to domestication.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":" ","pages":"123-135"},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10035640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of overexpression of KLF4 on the growth and development of hair follicles in mice. 过表达 KLF4 对小鼠毛囊生长和发育的影响

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-12-01 Epub Date: 2023-08-10 DOI: 10.1007/s00427-023-00708-8

Hongyu Han, Hong Qin, Yu Yang, Lijun Zhao, Tong Shen, Quanhai Pang

{"title":"Effect of overexpression of KLF4 on the growth and development of hair follicles in mice.","authors":"Hongyu Han, Hong Qin, Yu Yang, Lijun Zhao, Tong Shen, Quanhai Pang","doi":"10.1007/s00427-023-00708-8","DOIUrl":"10.1007/s00427-023-00708-8","url":null,"abstract":"Hair follicle growth is cyclical, and hair cycle dysfunction can lead to hair follicle-related disorders, including alopecia and hirsutism. The objective was to investigate the influence and underlying mechanism of Krüppel-like factor 4 (KLF4) overexpression on hair follicle growth and development in C57BL/6 mice. To provide a theoretical basis for the biological functions of KLF4 gene in hair follicle development and hair follicle cycle, mice were assigned to three groups: experimental, overexpressing KLF4 (Ad-KLF4); control, expressing green fluorescent protein (Ad-NC); and blank, no treatment. Fur was removed from the dorsal surface, and the mice were intradermally injected with 25 μL 1 × 1010 PFU/mL adenovirus vector (Ad-KLF4 or Ad-NC) at three points. Samples were collected for molecular biological and histological analysis. It was found that mRNA and protein levels of Wnt pathway-associated factors β-catenin, LEF1, hair follicle cell proliferation-related factor Ki67, and hair follicle inner caledrin marker AE15 were all significantly greater in the Ad-NC and blank groups than in Ad-KLF4 mice (P < 0.01). These findings were confirmed by immunohistochemical analysis. Hair growth was monitored photographically for 14 days, showing an absence of growth in the injected region of the KLF4-overexpressing mice in contrast to non-overexpressing areas where hair growth was normal. HE staining showed that hair follicles in the blank and Ad-NC mice were normal, while those in the KLF4-overexpressing areas remained in telogen or early anagen with spherical dermal papillae situated at the edge of the dermis and subcutaneous tissue without an inner heel sheath. In conclusion, it was found that KLF4 downregulated key Wnt/β-catenin-associated factors during follicular regeneration in mice, reducing both follicular development and growth.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":" ","pages":"137-145"},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10038189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in genome sequencing reveal changes in gene content that contribute to arthropod macroevolution. 基因组测序的进展揭示了节肢动物宏观进化过程中基因含量的变化。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-12-01 Epub Date: 2023-11-20 DOI: 10.1007/s00427-023-00712-y

Alys M Cheatle Jarvela, Judith R Wexler

引用次数: 0

Central projections from Johnston's organ in the locust: Axogenesis and brain neuroarchitecture. 蝗虫约翰斯顿器官的中枢投射：轴突生成与大脑神经结构

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-12-01 Epub Date: 2023-09-11 DOI: 10.1007/s00427-023-00710-0

George Boyan, Leslie Williams, Erica Ehrhardt

{"title":"Central projections from Johnston's organ in the locust: Axogenesis and brain neuroarchitecture.","authors":"George Boyan, Leslie Williams, Erica Ehrhardt","doi":"10.1007/s00427-023-00710-0","DOIUrl":"10.1007/s00427-023-00710-0","url":null,"abstract":"Johnston's organ (Jo) acts as an antennal wind-sensitive and/or auditory organ across a spectrum of insect species and its axons universally project to the brain. In the locust, this pathway is already present at mid-embryogenesis but the process of fasciculation involved in its construction has not been investigated. Terminal projections into the fine neuropilar organization of the brain also remain unresolved, information essential not only for understanding the neural circuitry mediating Jo-mediated behavior but also for providing comparative data offering insights into its evolution. In our study here, we employ neuron-specific, axon-specific, and epithelial domain labels to show that the pathway to the brain of the locust is built in a stepwise manner during early embryogenesis as processes from Jo cell clusters in the pedicel fasciculate first with one another, and then with the two tracts constituting the pioneer axon scaffold of the antenna. A comparison of fasciculation patterns confirms that projections from cell clusters of Jo stereotypically associate with only one axon tract according to their location in the pedicellar epithelium, consistent with a topographic plan. At the molecular level, all neuronal elements of the Jo pathway to the brain express the lipocalin Lazarillo, a cell surface epitope that regulates axogenesis in the primary axon scaffold itself, and putatively during fasciculation of the Jo projections to the brain. Central projections from Jo first contact the primary axon scaffold of the deutocerebral brain at mid-embryogenesis, and in the adult traverse mechanosensory/motor neuropils similar to those in Drosophila. These axons then terminate among protocerebral commissures containing premotor interneurons known to regulate flight behavior.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":" ","pages":"147-159"},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10258206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal plasticity of wing size and wing spot size in Drosophila guttifera. 果蝇翅膀大小和翅斑大小的热可塑性

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-12-01 Epub Date: 2023-06-19 DOI: 10.1007/s00427-023-00705-x

Yuichi Fukutomi, Aya Takahashi, Shigeyuki Koshikawa

{"title":"Thermal plasticity of wing size and wing spot size in Drosophila guttifera.","authors":"Yuichi Fukutomi, Aya Takahashi, Shigeyuki Koshikawa","doi":"10.1007/s00427-023-00705-x","DOIUrl":"10.1007/s00427-023-00705-x","url":null,"abstract":"Thermal plasticity of melanin pigmentation patterns in Drosophila species has been studied as a model to investigate developmental mechanisms of phenotypic plasticity. The developmental process of melanin pigmentation patterns on wings of Drosophila is divided into two parts, prepattern specification during the pupal period and wing vein-dependent transportation of melanin precursors after eclosion. Which part can be affected by thermal changes? To address this question, we used polka-dotted melanin spots on wings of Drosophila guttifera, whose spot areas are specified by wingless morphogen. In this research, we reared D. guttifera at different temperatures to test whether wing spots show thermal plasticity. We found that wing size becomes larger at lower temperature and that different spots have different reaction norms. Furthermore, we changed the rearing temperature in the middle of the pupal period and found that the most sensitive developmental periods for wing size and spot size are different. The results suggest that the size control mechanisms for the thermal plasticity of wing size and spot size are independent. We also found that the most sensitive stage for spot size was part of the pupal period including stages at which wingless is expressed in the polka-dotted pattern. Therefore, it is suggested that temperature change might affect the prepattern specification process and might not affect transportation through wing veins.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":" ","pages":"77-89"},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9657094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0