Development Genes and Evolution最新文献_第3页

Molecular characterization and expression analysis of a novel cold-inducible RNA-binding protein (CIRBP) gene in lamprey (Lethenteron reissneri). 一种新型冷诱导rna结合蛋白(CIRBP)基因在七鳃鳗(Lethenteron reissneri)中的表达分析。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-06-01 DOI: 10.1007/s00427-022-00700-8

Xuyuan Duan, Tong Pan, Zhuoying Wang, Yunhong Pu, Qingwei Li, Yue Pang, Yang Xu, Meng Gou

{"title":"Molecular characterization and expression analysis of a novel cold-inducible RNA-binding protein (CIRBP) gene in lamprey (Lethenteron reissneri).","authors":"Xuyuan Duan, Tong Pan, Zhuoying Wang, Yunhong Pu, Qingwei Li, Yue Pang, Yang Xu, Meng Gou","doi":"10.1007/s00427-022-00700-8","DOIUrl":"https://doi.org/10.1007/s00427-022-00700-8","url":null,"abstract":"Cold-inducible RNA-binding protein (CIRBP) responds to a wide array of cellular stresses such as cold shock, hypoxia, and inflammatory responses. However, functional studies of CIRBP in jawless vertebrates are limited. In this study, a CIRBP homolog from the jawless vertebrate lamprey (Lethenteron reissneri) was cloned and characterized (named Lr-CIRBP). The cDNA fragment of Lr-CIRBP has a 516 bp open reading frame (ORF) that encodes 171 amino acids, comprising a glycine-rich region at the C-terminal, similar to higher vertebrates but slightly shorter, and an RNA recognition motif (RRM) domain at the N-terminus. The predicted Lr-CIRBP sequence had 51.4 ~ 70.6% similarity with CIRBPs from other vertebrates. Further phylogenetic analysis revealed that Lr-CIRBP is located in the outgroup of vertebrates and is the ancestor of vertebrates. Based on real-time quantitative PCR experimental analysis, Lr-CIRBP expression was highest in leukocytes and increased significantly after multi-stimulation, peaking at 12 h. RNA interference showed that Lr-CIRBP knockdown can down-regulate the expression of inflammatory factors in Lethenteron reissneri. In conclusion, our study successfully clarifies the ancestral features and functions of CIRBP, while revealing valuable insight into how the protein is involved in the immune responses of a jawless vertebrate.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"233 1","pages":"49-57"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9623830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Duplicated zebrafish (Danio rerio) inositol phosphatases inpp5ka and inpp5kb diverged in expression pattern and function. 重复的斑马鱼（Danio rerio）肌醇磷酸酶 inpp5ka 和 inpp5kb 在表达模式和功能上存在差异。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-06-01 Epub Date: 2023-05-15 DOI: 10.1007/s00427-023-00703-z

Dhyanam Shukla, Brian M Gural, Edmund S Cauley, Namarata Battula, Shorbon Mowla, Brittany F Karas, Llion E Roberts, Luca Cavallo, Luka Turkalj, Sally A Moody, Laura E Swan, M Chiara Manzini

{"title":"Duplicated zebrafish (Danio rerio) inositol phosphatases inpp5ka and inpp5kb diverged in expression pattern and function.","authors":"Dhyanam Shukla, Brian M Gural, Edmund S Cauley, Namarata Battula, Shorbon Mowla, Brittany F Karas, Llion E Roberts, Luca Cavallo, Luka Turkalj, Sally A Moody, Laura E Swan, M Chiara Manzini","doi":"10.1007/s00427-023-00703-z","DOIUrl":"10.1007/s00427-023-00703-z","url":null,"abstract":"One hurdle in the development of zebrafish models of human disease is the presence of multiple zebrafish orthologs resulting from whole genome duplication in teleosts. Mutations in inositol polyphosphate 5-phosphatase K (INPP5K) lead to a syndrome characterized by variable presentation of intellectual disability, brain abnormalities, cataracts, muscle disease, and short stature. INPP5K is a phosphatase acting at position 5 of phosphoinositides to control their homeostasis and is involved in insulin signaling, cytoskeletal regulation, and protein trafficking. Previously, our group and others have replicated the human phenotypes in zebrafish knockdown models by targeting both INPP5K orthologs inpp5ka and inpp5kb. Here, we show that inpp5ka is the more closely related orthologue to human INPP5K. While both inpp5ka and inpp5kb mRNA expression levels follow a similar trend in the developing head, eyes, and tail, inpp5ka is much more abundantly expressed in these tissues than inpp5kb. In situ hybridization revealed a similar trend, also showing unique localization of inpp5kb in the pineal gland and retina indicating different transcriptional regulation. We also found that inpp5kb has lost its catalytic activity against its preferred substrate, PtdIns(4,5)P2. Since most human mutations are missense changes disrupting phosphatase activity, we propose that loss of inpp5ka alone can be targeted to recapitulate the human presentation. In addition, we show that the function of inpp5kb has diverged from inpp5ka and may play a novel role in the zebrafish.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"233 1","pages":"25-34"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9997629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formation of the brain by stem cell divisions of large neuroblasts in Oikopleura dioica, a simple chordate. 简单脊索动物双胸膜大神经母细胞干细胞分裂形成脑。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-06-01 DOI: 10.1007/s00427-023-00704-y

Misako Konishi, Kanae Kishi, Ryo Morita, Atsuko Yamada, Takeshi A Onuma, Hiroki Nishida

{"title":"Formation of the brain by stem cell divisions of large neuroblasts in Oikopleura dioica, a simple chordate.","authors":"Misako Konishi, Kanae Kishi, Ryo Morita, Atsuko Yamada, Takeshi A Onuma, Hiroki Nishida","doi":"10.1007/s00427-023-00704-y","DOIUrl":"https://doi.org/10.1007/s00427-023-00704-y","url":null,"abstract":"Stem cell division contributes to the generation of various cell types during animal development, especially a diverse pool of neural cells in the nervous system. One example is reiterated unequal stem cell divisions, in which a large stem cell undergoes a series of oriented unequal divisions to produce a chain of small daughter cells that differentiate. We show that reiterated unequal stem cell divisions are involved in the formation of the brain in simple chordate appendicularians (larvaceans). Two large neuroblasts in the anterior and middle of the brain-forming region of hatched larvae were observed. They produced at least 30 neural cells out of 96 total brain cells before completion of brain formation at 10 hours after fertilization by reiterated unequal stem cell divisions. The daughter cells of the anterior neuroblast were postmitotic, and the number was at least 19. The neuroblast produced small daughter neural cells posteriorly every 20 min. The neural cells first moved toward the dorsal side, turned in the anterior direction, aligned in a single line according to their birth order, and showed collective movement to accumulate in the anterior part of the brain. The anterior neuroblast originated from the right-anterior blastomeres of the eight-cell embryos and the right a222 blastomere of the 64-cell embryo. The posterior neuroblast also showed reiterated unequal stem cell divisions, and generated at least 11 neural cells. Sequential unequal stem cell divisions without stem cell growth have been observed in protostomes, such as insects and annelids. The results provide the first examples of this kind of stem cell division during brain formation in non-vertebrate deuterostomes.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"233 1","pages":"35-47"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9679414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complete dorsal structure is formed from only the blastocoel roof of Xenopus blastula: insight into the gastrulation movement evolutionarily conserved among chordates. 完整的背部结构仅由爪蟾囊胚顶形成:对脊索动物中进化保守的原肠胚形成运动的洞察。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-06-01 DOI: 10.1007/s00427-023-00701-1

Yuki Sato, Izumi Narasaki, Takuya Kunimoto, Yuki Moriyama, Chikara Hashimoto

{"title":"The complete dorsal structure is formed from only the blastocoel roof of Xenopus blastula: insight into the gastrulation movement evolutionarily conserved among chordates.","authors":"Yuki Sato, Izumi Narasaki, Takuya Kunimoto, Yuki Moriyama, Chikara Hashimoto","doi":"10.1007/s00427-023-00701-1","DOIUrl":"https://doi.org/10.1007/s00427-023-00701-1","url":null,"abstract":"Gastrulation is a critical event whose molecular mechanisms are thought to be conserved among vertebrates. However, the morphological movement during gastrulation appears to be divergent across species, making it difficult to discuss the evolution of the process. Previously, we proposed a novel amphibian gastrulation model, the \"subduction and zippering (S&Z) model\". In this model, the organizer and the prospective neuroectoderm are originally localized in the blastula's blastocoel roof, and these embryonic regions move downward to make physical contact of their inner surfaces with each other at the dorsal marginal zone. The developmental stage when contact between the head organizer and the anterior-most neuroectoderm is established is called \"anterior contact establishment (ACE).\" After ACE, the A-P body axis elongates posteriorly. According to this model, the body axis is derived from limited regions of the dorsal marginal zone at ACE. To investigate this possibility, we conducted stepwise tissue deletions using Xenopus laevis embryos and revealed that the dorsal one-third of the marginal zone had the ability to form the complete dorsal structure by itself. Furthermore, a blastocoel roof explant of the blastula, which should contain the organizer and the prospective neuroectoderm in the S&Z model, autonomously underwent gastrulation and formed the complete dorsal structure. Collectively, these results are consistent with the S&Z gastrulation model and identify the embryonic region sufficient for construction of the complete dorsal structure. Finally, by comparing amphibian gastrulation to gastrulation of protochordates and amniotes, we discuss the gastrulation movement evolutionarily conserved among chordates.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"233 1","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10239386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9677878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

BMP signaling is required to form the anterior neural plate border in ascidian embryos. 在海鞘胚胎中形成前神经板边界需要BMP信号。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2023-06-01 DOI: 10.1007/s00427-023-00702-0

Boqi Liu, Ximan Ren, Yutaka Satou

{"title":"BMP signaling is required to form the anterior neural plate border in ascidian embryos.","authors":"Boqi Liu, Ximan Ren, Yutaka Satou","doi":"10.1007/s00427-023-00702-0","DOIUrl":"https://doi.org/10.1007/s00427-023-00702-0","url":null,"abstract":"Cranial neurogenic placodes have been considered vertebrate innovations. However, anterior neural plate border (ANB) cells of ascidian embryos share many properties with vertebrate neurogenic placodes; therefore, it is now believed that the last common ancestor of vertebrates and ascidians had embryonic structures similar to neurogenic placodes of vertebrate embryos. Because BMP signaling is important for specifying the placode region in vertebrate embryos, we examined whether BMP signaling is also involved in gene expression in the ANB region of ascidian embryos. Our data indicated that Admp, a divergent BMP family member, is mainly responsible for BMP signaling in the ANB region, and that two BMP-antagonists, Noggin and Chordin, restrict the domain, in which BMP signaling is activated, to the ANB region, and prevent it from expanding to the neural plate. BMP signaling is required for expression of Foxg and Six1/2 at the late gastrula stage, and also for expression of Zf220, which encodes a zinc finger transcription factor in late neurula embryos. Because Zf220 negatively regulates Foxg, when we downregulated Zf220 by inhibiting BMP signaling, Foxg was upregulated, resulting in one large palp instead of three palps (adhesive organs derived from ANB cells). Functions of BMP signaling in specification of the ANB region give further support to the hypothesis that ascidian ANB cells share an evolutionary origin with vertebrate cranial placodes.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"233 1","pages":"13-23"},"PeriodicalIF":2.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9622175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Exploring the roles of FGF/MAPK and cVG1/GDF signalling on mesendoderm induction and convergent extension during chick primitive streak formation. 探讨FGF/MAPK和cVG1/GDF信号在鸡原始条纹形成过程中中胚层诱导和收敛扩展中的作用。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2022-12-01 DOI: 10.1007/s00427-022-00696-1

Hyung Chul Lee, Nidia M M Oliveira, Claudio D Stern

{"title":"Exploring the roles of FGF/MAPK and cVG1/GDF signalling on mesendoderm induction and convergent extension during chick primitive streak formation.","authors":"Hyung Chul Lee, Nidia M M Oliveira, Claudio D Stern","doi":"10.1007/s00427-022-00696-1","DOIUrl":"https://doi.org/10.1007/s00427-022-00696-1","url":null,"abstract":"During primitive streak formation in the chick embryo, cells undergo mesendoderm specification and convergent extension at the same time and in the same cells. Previous work has implicated cVG1 (GDF3) as a key factor for induction of primitive streak identity and positioning the primitive streak, whereas FGF signalling was implicated in regulating cell intercalation via regulation of components of the WNT-planar cell polarity (PCP) pathway. FGF has also been reported to be able to induce a primitive streak (but lacking the most axial derivatives such as notochord/prechordal mesendoderm). These signals emanate from different cell populations in the embryo, so how do they interact to ensure that the same cells undergo both cell intercalation and acquire primitive streak identity? Here we begin to address this question by examining in more detail the ability of the two classes of signals in regulating the two developmental events. Using misexpression of inducers and/or exposure to inhibitors and in situ hybridisation, we study how these two signals regulate expression of Brachyury (TBXT) and PRICKLE1 as markers for the primitive streak and the PCP, respectively. We find that both signals can induce both properties, but while FGF seems to be required for induction of the streak by cVG1, it is not necessary for induction of PRICKLE1. The results are consistent with cVG1 being a common regulator for both primitive streak identity and the initiation of convergent extension that leads to streak elongation.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"232 5-6","pages":"115-123"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10630076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early embryonic development of Johnston's organ in the antenna of the desert locust Schistocerca gregaria. 沙漠蝗虫（Schistocerca gregaria）触角中约翰斯顿器官的早期胚胎发育。

IF 0.8 3区生物学

Development Genes and Evolution Pub Date : 2022-12-01 Epub Date: 2022-09-23 DOI: 10.1007/s00427-022-00695-2

George Boyan, Erica Ehrhardt

{"title":"Early embryonic development of Johnston's organ in the antenna of the desert locust Schistocerca gregaria.","authors":"George Boyan, Erica Ehrhardt","doi":"10.1007/s00427-022-00695-2","DOIUrl":"10.1007/s00427-022-00695-2","url":null,"abstract":"Johnston's organ has been shown to act as an antennal auditory organ across a spectrum of insect species. In the hemimetabolous desert locust Schistocerca gregaria, Johnston's organ must be functional on hatching and so develops in the pedicellar segment of the antenna during embryogenesis. Here, we employ the epithelial cell marker Lachesin to identify the pedicellar domain of the early embryonic antenna and then triple-label against Lachesin, the mitosis marker phosphohistone-3, and neuron-specific horseradish peroxidase to reveal the sense-organ precursors for Johnston's organ and their lineages. Beginning with a single progenitor at approximately a third of embryogenesis, additional precursors subsequently appear in both the ventral and dorsal pedicellar domains, each generating a lineage or clone. Lineage locations are remarkably conserved across preparations and ages, consistent with the epithelium possessing an underlying topographic coordinate system that determines the cellular organization of Johnston's organ. By mid-embryogenesis, twelve lineages are arranged circumferentially in the pedicel as in the adult structure. Each sense-organ precursor is associated with a smaller mitotically active cell from which the neuronal complement of each clone may derive. Neuron numbers within a clone increase in discrete steps with age and are invariant between clones and across preparations of a given age. At mid-embryogenesis, each clone comprises five cells consolidated into a tightly bound cartridge. A long scolopale extends apically from each cartridge to an insertion point in the epithelium, and bundled axons project basally toward the brain. Comparative data suggest mechanisms that might also regulate the developmental program of Johnston's organ in the locust.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"232 5-6","pages":"103-113"},"PeriodicalIF":0.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9691482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10620826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Nodal-dependent enhancer of amphioxus Chordin sufficient to drive gene expression into the chordate dorsal organizer. 文昌鱼脊索蛋白节点依赖性增强子的鉴定足以驱动基因表达到脊索动物背侧组织中。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2022-12-01 DOI: 10.1007/s00427-022-00698-z

Simona Machacova, Zbynek Kozmik, Iryna Kozmikova

{"title":"Identification of Nodal-dependent enhancer of amphioxus Chordin sufficient to drive gene expression into the chordate dorsal organizer.","authors":"Simona Machacova, Zbynek Kozmik, Iryna Kozmikova","doi":"10.1007/s00427-022-00698-z","DOIUrl":"https://doi.org/10.1007/s00427-022-00698-z","url":null,"abstract":"The core molecular mechanisms of dorsal organizer formation during gastrulation are highly conserved within the chordate lineage. One of the key characteristics is that Nodal signaling is required for the organizer-specific gene expression. This feature appears to be ancestral, as evidenced by the presence in the most basally divergent chordate amphioxus. To provide a better understanding of the evolution of organizer-specific gene regulation in chordates, we analyzed the cis-regulatory sequence of amphioxus Chordin in the context of the vertebrate embryo. First, we generated stable zebrafish transgenic lines, and by using light-sheet fluorescent microscopy, characterized in detail the expression pattern of GFP driven by the cis-regulatory sequences of amphioxus Chordin. Next, we performed a 5'deletion analysis and identified an enhancer sufficient to drive the expression of the reporter gene into a chordate dorsal organizer. Finally, we found that the identified enhancer element strongly depends on Nodal signaling, which is consistent with the well-established role of this pathway in the regulation of the expression of dorsal organizer-specific genes across chordates. The enhancer identified in our study may represent a suitable simple system to study the interplay of the evolutionarily conserved regulatory mechanisms operating during early chordate development.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"232 5-6","pages":"137-145"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10620842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory breeding and rearing of cellar spider, Crossopriza lyoni Blackwall. 地窖蜘蛛的实验室选育与饲养。

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2022-12-01 DOI: 10.1007/s00427-022-00697-0

Johan Ariff Mohtar, Mohd Faidz Mohamad Shahimin

{"title":"Laboratory breeding and rearing of cellar spider, Crossopriza lyoni Blackwall.","authors":"Johan Ariff Mohtar, Mohd Faidz Mohamad Shahimin","doi":"10.1007/s00427-022-00697-0","DOIUrl":"https://doi.org/10.1007/s00427-022-00697-0","url":null,"abstract":"Spiders have emerged as one of the leading model organisms in many research fields due to their compelling biology. Often, scientific investigations involving the use of spiders face inevitable problems associated with the lack of specimens from laboratory stock, resulting in difficulties in yielding reproducible investigations for predictive research. Thus, several species of well-studied spiders, including Parasteatoda tepidariorum, have been successfully bred for such purposes. Crossopriza lyoni is a Haplogyne spider, globally distributed and widespread in human inhabitants, prompting interest in various studies over the last decades. Despite its scientific importance, no laboratory-bred C. lyoni has been documented. Therefore, we describe a successful captive breeding system of the species under controlled conditions to establish a laboratory stock culture. Methods for mating induction, egg collection and segregation, artificial embryo incubation, and colony husbandry are discussed. The technique presented is a simple and low-cost approach that is reliable for C. lyoni propagation in the laboratory over several generations.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"232 5-6","pages":"125-136"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10628864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cold acclimation diversity in Arabidopsis thaliana: CRISPR/Cas9 as a tool to fine analysis of Tandem Gene Arrays, application to CBF genes. 拟南芥冷驯化多样性:CRISPR/Cas9作为串联基因阵列精细分析的工具，在CBF基因中的应用

IF 2.4 3区生物学

Development Genes and Evolution Pub Date : 2022-12-01 DOI: 10.1007/s00427-022-00693-4

Carine Géry, Evelyne Téoulé

{"title":"Cold acclimation diversity in Arabidopsis thaliana: CRISPR/Cas9 as a tool to fine analysis of Tandem Gene Arrays, application to CBF genes.","authors":"Carine Géry, Evelyne Téoulé","doi":"10.1007/s00427-022-00693-4","DOIUrl":"https://doi.org/10.1007/s00427-022-00693-4","url":null,"abstract":"In this period of climate change, it is of major importance to increase knowledge about the mechanisms by whose plants adapt to their environment. Tandem gene arrays (TAG) are overrepresented in the pool of tandem duplicates involved in stress response and are consequently of special interest. Nevertheless, until recently, addressing questions about individual genes or fine regulations in such structures was very difficult due to the close arrangement of the genome, almost preventing the production of targeted simple or multiple mutants. The CRISPR/Cas9 new tool offers new opportunities as the setting of gene deletion strategies in various genetic backgrounds. Here, we used this technology on the cold acclimation CBF pathway in different accessions of Arabidopsis thaliana with the same set of guide RNAs. Deleted lines free of T-DNA have been produced for simple or multiple copies of CBF genes and evaluated for cold tolerance after acclimation. Expression levels of CBF genes and five COR genes have also been analyzed. Our data suggest first that two or three missing CBF genes are necessary to induce a strong reduction in cold tolerance and secondly that most deletions have a low impact on the expression of remaining CBF copies which contradicts the previous hypothesis in the literature. Our results thus show that the CRISPR/Cas9 deletion strategy is a useful performance tool to investigate how tandem gene arrays work.","PeriodicalId":50588,"journal":{"name":"Development Genes and Evolution","volume":"232 5-6","pages":"147-154"},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10634393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1