Nicole Jahnsen-Guzmán , Nelson A. Lagos , Marco A. Lardies , Pedro A. Quijón , Diego Quintanilla-Ahumada , M. Roberto García-Huidobro , Claudio García , Nicol Zúñiga-Cueto , Cristian Duarte
{"title":"Projected cooling and pCO2 conditions in upwelling zones and their influence on a prominent rocky shore ecosystem engineer","authors":"Nicole Jahnsen-Guzmán , Nelson A. Lagos , Marco A. Lardies , Pedro A. Quijón , Diego Quintanilla-Ahumada , M. Roberto García-Huidobro , Claudio García , Nicol Zúñiga-Cueto , Cristian Duarte","doi":"10.1016/j.ecss.2025.109531","DOIUrl":"10.1016/j.ecss.2025.109531","url":null,"abstract":"<div><div>By the end of the century, upwelling zones are expected to undergo distinct changes due to the accumulation of greenhouse gases in the atmosphere. These changes include an intensification of the winds causing upwelling, further reducing sea surface temperatures (cooling), and an intensification of ocean acidification (OA). While only a few studies have evaluated the influence of cooling conditions in these systems, even fewer have assessed the combined effects of cooling and projected OA. This study addressed this gap by exposing juveniles of the intertidal purple mussel (<em>Perumytilus purpuratus</em>), a prominent intertidal ecosystem engineer, to distinct temperatures and <em>p</em>CO<sub>2</sub> levels. Using a mesocosm system and a 2 × 2 factorial design, groups of purple mussels were exposed to current (15 °C) and projected cooling conditions (10 °C), and current and projected <em>p</em>CO<sub>2</sub> levels (500 and 1500 μatm, respectively). After two months, we quantified mussel growth, calcification, byssus thread production, clearance, and metabolic rates. Growth, calcification, and byssus thread production rates were consistently affected by temperature and by the interaction between temperature and <em>p</em>CO<sub>2</sub>: At current temperatures (15 °C) all these variables increased in response to OA, but when exposed to projected cooling conditions (10 °C), these trends reversed and declined with OA. Mussel clearance rates followed the same trend, but in this case the only significant factor was the interaction between variables. Meanwhile, metabolic rates declined with temperature. A close examination of the variation among treatments suggests that the main changes were consistently associated with a sharp decline in most response variables to a combination of cooling and high <em>p</em>CO<sub>2</sub> conditions. Hence, projected end of the century cooling and OA are likely to have direct (negative) effects on this habitat-forming species. Indirectly, the combination of these stressors may weaken mussel bed structure and reduce habitat complexity, thereby halting the benefits provided to associated intertidal communities.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"326 ","pages":"Article 109531"},"PeriodicalIF":2.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yi Wei , Kui Wang , Haiyan Jin , Wenbin Yin , Daji Huang , Jiliang Xuan , Bin Wang , Feng Zhou , Jianfang Chen
{"title":"Intermittent intrusions of Changjiang Diluted water in Hangzhou Bay modulated by intraseasonal winds and tropical cyclones","authors":"Yi Wei , Kui Wang , Haiyan Jin , Wenbin Yin , Daji Huang , Jiliang Xuan , Bin Wang , Feng Zhou , Jianfang Chen","doi":"10.1016/j.ecss.2025.109525","DOIUrl":"10.1016/j.ecss.2025.109525","url":null,"abstract":"<div><div>It is generally recognised that the north-easterly monsoon leads to greater intrusion of Changjiang Diluted Water (CDW) into Hangzhou Bay in winter than in summer, which strongly influences the hydrography of Hangzhou Bay. However, anomalously lower salinity (<10) and higher dissolved inorganic nitrogen (DIN) concentrations (1.9 mg L<sup>−1</sup>) were observed in central Hangzhou Bay in summer (August 2019) than the salinity (>15) and DIN concentrations (1.5 mg L<sup>−1</sup>) in winter (March 2022). A high-resolution, well-validated model has further revealed intermittent intrusions of CDW, characterised as intraseasonal (30–120 d) and episodic variations, resulting in a unique low-salinity and high-nutrient water mass in the north-central bay during summer. The intermittent intrusions are driven by the combined effects of the Changjiang discharge, intraseasonal winds, tropical cyclones, and tidal residual currents. The Changjiang discharge amplifies the effect of CDW intrusion on a seasonal scale, which is responsible for the large (>10) decline in summer salinity in the initial intrusion area of the northern bay mouth. The intraseasonal variation in the south-westerly monsoon in summer causes intermittent intrusion of the CDW, accompanied by variations in the Taiwan Warm Current. Intrusion of CDW is greatly enhanced during episodic events of strong northerly winds caused by tropical cyclones. This episodic intrusion can cause a decline in salinity in the initial intrusion area of up to 6 in approximately one week. After the CDW intrudes into the north central bay, the westward tidal residual current along the northern coast continuously transports the CDW toward the head of Hangzhou Bay.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"326 ","pages":"Article 109525"},"PeriodicalIF":2.6,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mangrove-induced landward sediment transport owing to sediment flux asymmetry and bed shear stress","authors":"Guangwei Huang, Tongtiegang Zhao","doi":"10.1016/j.ecss.2025.109527","DOIUrl":"10.1016/j.ecss.2025.109527","url":null,"abstract":"<div><div>Mangrove-induced tidal asymmetries lead to complex patterns of sediment transport. This paper aims to detail mangrove-induced landward sediment fluxes by numerical modelling. Specifically, using rigid cylinders to generalize mangrove forests and account for geometric and morphometric characteristics, mangrove-induced asymmetries in duration, velocity and sediment flux are explicitly considered. A case study is devised for Shenzhen Bay considering its unique status as the only mangrove nature reserve located in the hinterland of the modern metropolis in China. The results under the idealized bay model reveal that mangrove forests induce asymmetric tidal durations, i.e., shorter flood tides and longer ebb tides. The tidal duration asymmetry induces tidal velocity asymmetry, characterized by faster flood tides and slower ebb tides, leading to differences in bed shear stress. The peak difference in bed shear stress contributes to landward-dominant sediment fluxes. Higher bed shear stress during flood tides transports sediment landward, whereas lower bed shear stress during ebb tides fails to induce sediment resuspension. During flood tides, the maximum suspended sediment concentration exceeds that during ebb tides by ∼0.676 kg/m<sup>3</sup> in the middle of the bay, ∼0.789 kg/m<sup>3</sup> outside mangrove forests and ∼0.015 kg/m<sup>3</sup> inside mangrove forests. Sediment accumulates at the fringe and also inside mangrove forests, eventually raising bed elevation over time. In the meantime, increasing water depth attenuates tidal asymmetry in duration, velocity and sediment flux, whereas the landward-dominant asymmetry persists. Overall, this paper disentangles the integrated process of mangrove-induced landward sediment fluxes by considering sediment flux asymmetry and bed shear stress.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"326 ","pages":"Article 109527"},"PeriodicalIF":2.6,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ligia Salgado Bechara , Rafael Monteiro Duarte , Nícolas Tomaz Santana , Gustavo Mattos , Arthur de Barros Bauer , Maurício Mussi Molisani , Luciano Gomes Fischer , Carlos Eduardo de Rezende , Yasmina Shah Esmaeili , Patrícia Luciano Mancini , Carlos Alberto de Moura Barboza
{"title":"Ontogenetic trophic variation in ghost crab Ocypode quadrata","authors":"Ligia Salgado Bechara , Rafael Monteiro Duarte , Nícolas Tomaz Santana , Gustavo Mattos , Arthur de Barros Bauer , Maurício Mussi Molisani , Luciano Gomes Fischer , Carlos Eduardo de Rezende , Yasmina Shah Esmaeili , Patrícia Luciano Mancini , Carlos Alberto de Moura Barboza","doi":"10.1016/j.ecss.2025.109509","DOIUrl":"10.1016/j.ecss.2025.109509","url":null,"abstract":"<div><div>Changes in food resource use over an organism's lifespan play a pivotal role in structuring trophic webs. Although substantial evidence suggests that many benthic invertebrate species undergo ontogenetic dietary shifts, this process remains scarcely studied in sandy beach species. <em>Ocypode quadrata</em> is the unique ghost crab species in the Western Atlantic and exhibits generalist feeding habits, playing a key role in energy transfer across trophic levels and connecting coastal ecosystems. We analyzed stomach contents and <em>δ</em><sup>15</sup>N and <em>δ</em><sup>13</sup>C stable isotopes from juveniles and adults of <em>O. quadrata</em> to test the hypothesis of size-dependent niche shifts. Sediment and unidentified organic matter were the most commonly detected items in stomach contents. Adults consumed the mole crab <em>Emerita brasilienis</em> more frequently (63 %), a prey absent in juvenile stomachs, whereas insects were more prevalent in juveniles (78 %). Microplastics were detected in over one-third of juveniles, indicating their exposure to marine litter contamination. Similar <em>δ</em><sup>15</sup>N values suggest that adults and juveniles occupy similar trophic levels, sharing a large portion of their diet, though assimilation frequencies or magnitudes may differ. Our results support the hypothesis that adults have a broader trophic niche than juveniles, likely reflecting greater ability in exploiting a wider range of food resources. The broader isotopic niche in adults aligns with their higher consumption of <em>E. brasilienis</em>. The assimilation of both marine and terrestrial resources reinforces the meta-ecosystem framework, highlighting the ecological importance of allochthonous inputs to sandy beach ecosystems and underscoring the need for landscape-scale conservation strategies.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"326 ","pages":"Article 109509"},"PeriodicalIF":2.6,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kyle A. Emery , Samuel G. Rivera , Andrew Pettit , Jessica R. Madden , Nicholas K. Schooler , David M. Hubbard , Jenifer E. Dugan
{"title":"Spatial patterns of sandy beach habitat use by mobile invertebrates vary with wrack type and tide phase","authors":"Kyle A. Emery , Samuel G. Rivera , Andrew Pettit , Jessica R. Madden , Nicholas K. Schooler , David M. Hubbard , Jenifer E. Dugan","doi":"10.1016/j.ecss.2025.109510","DOIUrl":"10.1016/j.ecss.2025.109510","url":null,"abstract":"<div><div>Habitat distributions of many coastal species are expected to shift with climate change and sea level rise, affecting local resource use and biotic interactions. Intertidal zones of sandy beaches support a diversity of highly mobile invertebrates that move frequently to adjust to changing tides as well as habitat availability and condition. Many of these species rely on allochthonous inputs of marine macrophytes, or wrack, for food and shelter. We evaluated the effects of habitat availability and resources on spatial niche partitioning by macroinvertebrate detritivores and predators using field surveys and a field experiment on wrack colonization and consumption. Semi-lunar tide phase greatly affected the amount of suitable habitat (54 % more on spring tide) and the mean position of wrack subsidies on the beach (34 % more landward). Predator and detritivore distributions overlapped, but predators were concentrated higher on the beach. From neap to spring tide, the mean intertidal positions of wrack detritivores expanded more (biomass 49.8 %; abundance 45.4 %) than predator positions. In the experiment, significantly more kelp wrack, <em>Egregia menziesii</em> and <em>Macrocystis pyrifera</em>, was consumed than seagrass wrack, <em>Phyllospadix torreyi,</em> at all intertidal levels. Wrack-associated invertebrate community composition differed among wrack types and intertidal levels. Our findings demonstrate how highly mobile intertidal macroinvertebrates partition beach habitat and food resources, with notable spatial separation between detritivores and predators, and how this behavior can be mediated by tide phase and wrack type and position. Linking spatial dynamics of mobile intertidal animals to future projections of habitat availability and resource supply can inform management of sandy beaches with relevance for their conservation as ecosystems in the face of global change.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"326 ","pages":"Article 109510"},"PeriodicalIF":2.6,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Robert A.B. Mason , Clothilde Langlais , Julian Uribe-Palomino , Mark Tonks , Frank Coman , Severine Choukroun , Javier Porobic , Christopher Doropoulos
{"title":"Reef-scale variation in larval supply and settlement: validating Lagrangian dispersal predictions with observations of coral larvae","authors":"Robert A.B. Mason , Clothilde Langlais , Julian Uribe-Palomino , Mark Tonks , Frank Coman , Severine Choukroun , Javier Porobic , Christopher Doropoulos","doi":"10.1016/j.ecss.2025.109506","DOIUrl":"10.1016/j.ecss.2025.109506","url":null,"abstract":"<div><div>Population persistence and recovery in marine systems is driven by larval dispersal in the water column, generating ecological connectivity between the natal and settlement locations. Connectivity modelling is commonly utilised for the spatial planning of marine protected areas and, more recently, for the prioritisation of restoration interventions. Here, we conducted field experiments to validate the spatial patterns and rates of larval arrival as simulated by a high-resolution connectivity model (∼300 m resolution), using complementary spatial and temporal sampling of coral larvae and newly settled recruits around a cluster of offshore coral reefs. At the within-reef scale, Lagrangian dispersal modelling demonstrated only a fair performance at predicting observed spatial patterns of larval arrival and settlement, at best. However, at the reef cluster level, hydrodynamically-driven interannual variations in larval supply were well correlated with observed interannual variations. Combined, the model results resolve empirical observations for the temporal (inter-annual) and spatial scales relevant to meta-population dynamics (1–10s of km's). At the finer spatial scales of resolution (>1/10 ha to <10 ha), relevant to current restoration interventions, skill at predicting larval density is poor whilst skill at predicting larval settlement is fair. Overall, our findings identify the need for a model validation framework that considers the scales of physical processes resolved by the hydrodynamic modelling, spatial-temporal variability in the propagule populations being measured, the error tolerance for how the outputs of model simulations are being utilised (theoretical <em>versus</em> operational), and the complementary use of modelling and field sampling for different scales of application.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"326 ","pages":"Article 109506"},"PeriodicalIF":2.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maren Voss , Peter Holtermann , Soeren Ahmerkamp , Damian Leonardo Arévalo Martínez , Bronwyn Cahill , Manita Chouksey , Joachim W. Dippner , Jörg Dutz , Peter Feldens , Jacob Geersen , Kaja Gentsch , Mayya Gogina , Daniel Herlemann , Jan Henkel , Marion Kanwischer , Bennet Krebs , Anke Kremp , Anju Malissery , Robert Mars , Jann Müller , Oliver Zielinski
{"title":"Coastal zones of the Baltic Sea in the Anthropocene: Current state and the impact of climate change","authors":"Maren Voss , Peter Holtermann , Soeren Ahmerkamp , Damian Leonardo Arévalo Martínez , Bronwyn Cahill , Manita Chouksey , Joachim W. Dippner , Jörg Dutz , Peter Feldens , Jacob Geersen , Kaja Gentsch , Mayya Gogina , Daniel Herlemann , Jan Henkel , Marion Kanwischer , Bennet Krebs , Anke Kremp , Anju Malissery , Robert Mars , Jann Müller , Oliver Zielinski","doi":"10.1016/j.ecss.2025.109504","DOIUrl":"10.1016/j.ecss.2025.109504","url":null,"abstract":"<div><div>Coastal zones and estuaries are highly dynamic marine systems subject to anthropogenic pressure and to climate-related changes. The coastal zone of the Baltic Sea, shaped by extreme seasonality, strong bentho-pelagic coupling, and intense human use, represents a unique model system to study these interactions under accelerating climate change. While extensive research has been conducted on warming, eutrophication, and large scale hypoxia in the region, critical gaps remain in understanding how physical forcing, sediment type, and benthic–pelagic coupling control nutrient turnover, primary production, seed and egg bank dynamics, and the emission of climate-relevant gases under rapidly changing dynamic conditions. The Baltic Sea, with 26 % of its area shallower than 15 m, harbor mostly sandy sediments along the southern coast, but the coastal nutrient filter remains poorly investigated because element fluxes are mostly controlled by advection. Sediments are home for phytoplankton resting stages, but the contribution of seed germination to the development of phytoplankton blooms is unknown. The resting eggs of zooplankton are also deposited in the sediment, but the timing of zooplankton and fish development in spring and the impact of progressive warming are poorly understood. Decreases in salinity strongly influence macrobenthos in the Baltic Sea, whereas increased eutrophication favors the growth of opportunistic species. Finally, coastal darkening is discussed, as it affects marine life in ways that are difficult to assess. Future studies of coastal zones will benefit from technical innovations like mooring systems that transmits data immediately to the shore and uses drones to aid in sampling along coastlines. This review synthesizes current knowledge on the state and functioning of the Baltic coastal zone, highlighting novel insights into the role of permeable sandy sediments as nutrient cycling hotspots. We identify key uncertainties arising from the high spatio-temporal variability of these systems, which limit the predictive capacity of existing models. Only by combining novel approaches we can improve projections of climate-change impacts, and provide a robust scientific basis for the management and protection of vulnerable coastal ecosystems. Accordingly, the measures that will enable stakeholders and politicians to improve the protection of coastal areas remain to be developed.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"326 ","pages":"Article 109504"},"PeriodicalIF":2.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Length-weight relationship of the bony fish community inhabiting the southwest Atlantic coast, with emphasis on commercially important species","authors":"Ailin Monti , Claudio Ruarte , Paula Orlando , Mariano Elisio","doi":"10.1016/j.ecss.2025.109508","DOIUrl":"10.1016/j.ecss.2025.109508","url":null,"abstract":"<div><div>Knowledge of the parameters of the fish length-weight relationship model (LWR), <span><math><mrow><mi>W</mi><mi>e</mi><mi>i</mi><mi>g</mi><mi>h</mi><mi>t</mi><mo>=</mo><mi>a</mi><mspace></mspace><mo>∗</mo><mspace></mspace><mi>L</mi><mi>e</mi><mi>n</mi><mi>g</mi><mi>h</mi><msup><mi>t</mi><mrow><mspace></mspace><mi>b</mi></mrow></msup></mrow></math></span>, provides essential information for understanding biology and population assessments, enabling the development of conservation measures and fisheries management. This study estimated LWR parameters and described the growth type of 46 bony fish species (from 28 families and 43 genera) caught by bottom trawl in the fishery assessment surveys carried out by the National Institute of Fisheries Research and Development between 1981 and 2022 on the southwest Atlantic coast between 34° and 42°S, <50 m depth. The LWR parameters (a and b) were estimated by fitting linear regression models to the total length and weight data after log transformation. Growth type was assessed by statistically comparing the parameter b with a value of 3 (isometric growth), and considering a negative or positive allometric growth when b < 3 or b > 3, respectively. Model fit was statistically significant for all species (R<sup>2</sup> = 0.83–0.99). Half of the species evaluated exhibited negative allometric growth, 35 % positive allometric, and 15 % isometric growth. Among the 24 commercially important species, nearly half showed positive allometric growth. Thus, most of these bony fishes from the southwest Atlantic coast appear to fail the cube law of isometric growth. This study publishes for the first time information on this important biological trait for 42 species along the assessed region, four of which had no data available worldwide, and updates the available information for a further four species, improving knowledge for future biological and ecological assessments of their populations.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"325 ","pages":"Article 109508"},"PeriodicalIF":2.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Coastal fauna under threat from roadkills: A case study in southeastern Brazil","authors":"Camyla Freitas Viana , Eduardo Bulhões , Danilo Freitas Rangel , Ilana Rosental Zalmon , Leonardo Lopes Costa","doi":"10.1016/j.ecss.2025.109507","DOIUrl":"10.1016/j.ecss.2025.109507","url":null,"abstract":"<div><div>Animal collisions serve as key indicators of the impacts of roads on biodiversity. However, existing records and predictions of roadkill susceptibility predominantly focus on large inland roads and terrestrial species, overlooking its broader impacts on diverse ecosystems and coastal fauna. The objective of this study was to evaluate whether spatial and temporal factors influence the risk of animals being road-killed on a coastal road in Southeastern Brazil. The road was monitored from August 2021 to January 2024 over a stretch of approximately 8 km. The search for animals on the road was conducted at night using a vehicle traveling at ∼20 km/h. Each animal encounter was georeferenced to measure satellite-based variables including the level of urbanization, artificial light, shoreline tree coverage, beach width, coastline retreat tendency, tide range, air temperature, precipitation, and wave height. A total of 250 animals were found, including nine opossums, six birds, three snakes, one bat, 47 sea turtle hatchlings, and 184 ghost crabs. According to the Generalized Linear Model with binomial distribution, the risk of ghost crabs being road-killed was significantly higher in areas more affected by erosion, and during storm wave events. These results provide evidence that coastal roadkills are intensified by the loss of refuge habitats, a key characteristic of the coastal squeeze phenomenon. To minimize wildlife collisions on coastal roads, it is recommended to close roads during storms mainly in areas affected by erosion, build wildlife crossings, install signs indicating the presence of fauna, and implement public awareness programs in the region.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"325 ","pages":"Article 109507"},"PeriodicalIF":2.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"RNA-seq and qPCR analyses of food conditions and their influence on gene expression in the Manila clam (Ruditapes philippinarum)","authors":"Nariaki Inoue , Natsumi Sano , Shouji Houki","doi":"10.1016/j.ecss.2025.109505","DOIUrl":"10.1016/j.ecss.2025.109505","url":null,"abstract":"<div><div>An understanding of the food conditions to which clams are exposed and the associated differences in physiologyical conditions is important for identifying the mechanisms that affect resource fluctuations. We performed RNA-seq and quantitative PCR with juveniles reared under two different feeding conditions: non-feeding (N) and feeding (F). RNA-seq with 18 libraries contained a total of 167733 unigenes of which 6093 were determined to be differentially expressed genes (DEGs) in the comparison of N and F on days 3, 5 and 20 during the rearing period. From the pathway analysis of DEGs, ECM-receptor interaction and Focal adhesion pathways were well enriched, and they are characteristic pathways that respond to changes in food conditions. From changes in the defensin, ependymin-related protein-1, heat shock protein 22 and thioredoxin expression patterns, we could categorize the physiological conditions of juveniles in starvation into short-term starvation (1–3 days), during which genes expression in the non-feeding (N) relative to the feeding (F) declined rapidly; medium-term starvation (3–15 days), during which genes expression then increased rapidly; and long-term starvation (15 days and longer), during which genes expression declined again.</div></div>","PeriodicalId":50497,"journal":{"name":"Estuarine Coastal and Shelf Science","volume":"325 ","pages":"Article 109505"},"PeriodicalIF":2.6,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}