Experimental and Toxicologic Pathology最新文献_第6页

Progression of renal fibrosis in congenital CKD model rats with reduced number of nephrons. 肾单位数量减少的先天性CKD模型大鼠肾纤维化的进展。

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.01.007

H. Yasuda, Y. Tochigi, K. Katayama, Hiroetsu Suzuki

引用次数: 5

A combined approach to early detect in vitro drug-induced hemostatic changes in preclinical safety. 联合方法早期检测体外药物致止血变化的临床前安全性。

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.01.010

M. Defontis, S. Côté, D. Ledieu

引用次数: 2

Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on brush border membrane enzymes, carbohydrate metabolism and antioxidant system in rat intestine 黑皮油可改善顺铂对大鼠肠道刷缘膜酶、碳水化合物代谢及抗氧化系统的影响

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.02.001

Faaiza Shahid , Zeba Farooqui , Sana Rizwan , Subuhi Abidi , Iqbal Parwez , Farah Khan

{"title":"Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on brush border membrane enzymes, carbohydrate metabolism and antioxidant system in rat intestine","authors":"Faaiza Shahid , Zeba Farooqui , Sana Rizwan , Subuhi Abidi , Iqbal Parwez , Farah Khan","doi":"10.1016/j.etp.2017.02.001","DOIUrl":"https://doi.org/10.1016/j.etp.2017.02.001","url":null,"abstract":"<div>Cisplatin (CP) is an effective chemotherapeutic agent that induces gastrointestinal toxicity. Nigella sativa oil (NSO) has been shown to be beneficial in a wide range of gastrointestinal disorders. The present study investigates the possible protective effect of NSO on CP-induced gastrointestinal toxicity. NSO administration (2 ml/kg bwt, orally), prior to and following, a single dose CP treatment (6 mg/kg bwt. ip), significantly attenuated the CP-induced decrease in brush border membrane (BBM) enzyme activities in intestinal homogenates and BBM vesicles (BBMV). NSO administration also mitigated CP induced alterations in the activities of carbohydrate metabolism enzymes and in the enzymatic and non-enzymatic antioxidant parameters in the intestine. The results suggest that NSO by empowering the endogenous antioxidant system improves intestinal redox and metabolic status and restores BBM integrity in CP treated rats. Histopathological studies supported the biochemical findings. Thus, NSO may help prevent the accompanying gastrointestinal dysfunction in CP chemotherapy.</div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 299-306"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71830060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Metaplastic phenotype in human primary bronchiolar epithelial cells after repeated exposure to native mainstream smoke at the air-liquid interface 人原发性细支气管上皮细胞反复暴露于空气-液体界面的天然主流烟雾后的化生表型

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.01.015

Michaela Aufderheide , Shigeaki Ito , Shinkichi Ishikawa , Makito Emura

{"title":"Metaplastic phenotype in human primary bronchiolar epithelial cells after repeated exposure to native mainstream smoke at the air-liquid interface","authors":"Michaela Aufderheide , Shigeaki Ito , Shinkichi Ishikawa , Makito Emura","doi":"10.1016/j.etp.2017.01.015","DOIUrl":"10.1016/j.etp.2017.01.015","url":null,"abstract":"<div>3D constructs composed of primary normal differentiated human bronchiolar epithelial (NHBE) cells as mono- or co-culture in combination with normal human lung fibroblasts were exposed repeatedly at the air-liquid interface with non-lethal concentrations of mainstream cigarette smoke (4 cigarettes a day, 5 days/week, 13 times repetition in total) to build up a permanent burden on the cells. Samples were taken after 4, 8 and 13 times of repeated smoke exposure and the cultures were analyzed by histopathological methods In comparison with the clean air exposure (process control) and incubator control cells the cigarette smoke exposed cultures showed a reduction of cilia bearing as well as mucus producing cells. In both mono- as well as co-cultures, hyperplasia was induced showing different histological cell types (undifferentiated secretory and squamous cell types). At the end of the exposure phase, we observed the development of non-hyperplastic areas strongly positive to CK13 antibody, commonly seen in squamous cells as a marker for non-cornified squamous epithelium, thus suggesting a transition of the normal bronchial epithelial cells towards metaplastic cells. The control cultures (clean air exposed and incubator cells) showed no comparable phenotypic changes. In conclusion, our in vitro model presents a valuable tool to study the induction of metaplastic alterations after exposure to airborne material.</div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 307-315"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.01.015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34777401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

The potential protective role of taurine against 5-fluorouracil-induced nephrotoxicity in adult male rats. 牛磺酸对5-氟尿嘧啶引起的成年雄性大鼠肾毒性的潜在保护作用。

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.01.012

H. Yousef, H. Aboelwafa

引用次数: 21

Cytogenotoxicological defense of retinyl palmitate in the front damage of antineoplastics. 棕榈酸视黄醇在抗肿瘤前损伤中的细胞基因毒理学防御。

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.01.013

Ricardo Melo de Carvalho, R. P. Aguiar, M. T. Islam, M. A. D. de Alencar, Ana Maria oliveira da Mata, A. L. Braga, Josemar José da Silva Júnior, L. R. Sousa, Rosália Maria Tôrres de Lima, M. F. C. J. Paz, J. M. C. E. E Sousa, A. A. Melo-Cavalcante

引用次数: 8

Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on brush border membrane enzymes, carbohydrate metabolism and antioxidant system in rat intestine. 黑皮油可改善顺铂对大鼠肠道刷缘膜酶、碳水化合物代谢及抗氧化系统的影响。

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.02.001

F. Shahid, Z. Farooqui, S. Rizwan, S. Abidi, I. Parwez, F. Khan

引用次数: 31

Progression of renal fibrosis in congenital CKD model rats with reduced number of nephrons 肾单位数量减少的先天性CKD模型大鼠肾纤维化的进展

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.01.007

Hidenori Yasuda, Yuki Tochigi, Kentaro Katayama, Hiroetsu Suzuki

{"title":"Progression of renal fibrosis in congenital CKD model rats with reduced number of nephrons","authors":"Hidenori Yasuda, Yuki Tochigi, Kentaro Katayama, Hiroetsu Suzuki","doi":"10.1016/j.etp.2017.01.007","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.007","url":null,"abstract":"<div>A congenital reduction in the number of nephrons is a critical risk factor for both onset of chronic kidney disease (CKD) and its progression to end-stage kidney disease (ESKD). Hypoplastic kidney (HPK) rats have only about 20% of the normal number of nephrons and show progressive CKD. This study used an immunohistological method to assess glomerular and interstitial pathogenesis in male HPK rats aged 35–210 days. CD68 positive-macrophages were found to infiltrate into glomeruli in HPK rats aged 35 and 70   days showed glomerular hypertrophy, loss of normal linear immunostaining of podocine, and increased expression of PDGFr-β, TGF-β, collagens, and fibronectin, with all of these alterations gradually deteriorating with age. α-SMA-positive myofibroblasts were rarely detected in glomerular tufts, whereas α-SMA-positive glomerular parietal epithelium (GPE) cells were frequently observed along Bowman’s capsular walls. The numbers of PDGFr-β-positive fibroblasts in interstitial tissue were increased in rats aged 35 days and older, whereas interstitial fibrosis, characterized by the increased expression of tubular PDGF-BB, the appearance of myofibroblasts doubly positive for PDGFr-β and α-SMA, and increased expression of collagens and fibronectin, were observed in rats aged 70 and older. These results clearly indicate that congenital CKD with only 20% of nephrons cause renal fibrosis in rats.</div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 245-258"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.01.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71830055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A spontaneous high-grade undifferentiated mammary carcinoma in a seven-week-old female rat 7周龄雌性大鼠自发性高级别未分化乳腺癌

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.01.006

Ana I. Faustino-Rocha , Adelina Gama , Paula A. Oliveira , Antonieta Alvarado , Rita Ferreira , Mário Ginja

引用次数: 2

Pathological and biochemical evaluation of coumarin and chlorophyllin against aflatoxicosis in rat. 香豆素和叶绿素抗黄曲霉中毒大鼠的病理及生化评价。

Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI: 10.1016/j.etp.2017.01.014

M. S. Abdel-Latif, K. Elmeleigy, T. Aly, M. Khattab, S. M. Mohamed

引用次数: 17