Experimental and Toxicologic Pathology最新文献

{"title":"A combined approach to early detect in vitro drug-induced hemostatic changes in preclinical safety.","authors":"M. Defontis, S. Côté, D. Ledieu","doi":"10.1016/j.etp.2017.01.010","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.010","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"52 1","pages":"275-283"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75001610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of silymarin and curcumin on cyclophosphamide-induced cardiotoxicity","authors":"H. Avci ,&nbsp;E.T. Epikmen ,&nbsp;E. Ipek ,&nbsp;R. Tunca ,&nbsp;S.S. Birincioglu ,&nbsp;H. Akşit ,&nbsp;S. Sekkin ,&nbsp;A.N. Akkoç ,&nbsp;M. Boyacioglu","doi":"10.1016/j.etp.2017.02.002","DOIUrl":"10.1016/j.etp.2017.02.002","url":null,"abstract":"<div><h3>Introduction</h3><p>Cyclophosphamide<span><span> (CP) is a potent anticancer agent; its clinical use is limited due to its marked </span>cardiotoxicity.</span></p></div><div><h3>Aim</h3><p><span>The present study was aimed at evaluating the cardioprotective effects of </span>silymarin<span> (SLY) and curcumin (CUR), which have strong antioxidant properties, against the toxic effects of high-dose CP on the heart of rats.</span></p></div><div><h3>Materials and methods</h3><p><span>A total of 36 adult Wistar albino female rats were randomly divided into six groups. Group I (control group; nothing was administered), Group II (CP group; 30</span> <!-->mg/kg/day CP was administered intraperitoneally to each animal for seven days), Group III (SLY group; 100<!--> <!-->mg/kg/day SLY by gavage for 14 days), Group IV (CUR group; 100<!--> <!-->mg/kg/day CUR by gavage for 14 days), Group V (SLY<!--> <!-->+<!--> <!-->CP group; 100<!--> <!-->mg/kg/day SLY by gavage for 14<!--> <!-->days plus 30<!--> <!-->mg/kg/day CP intraperitoneally starting from the seventh day) and Group VI (CUR<!--> <!-->+<!--> <!-->CP group; 100<!--> <!-->mg/kg/day CUR by gavage for 14<!--> <!-->days plus 30<!--> <!-->mg/kg/day CP intraperitoneally starting from the seventh day). Biochemical, histopathological and immunohistochemical methods were utilised for evaluation of the cardiotoxicity.</p></div><div><h3>Results</h3><p><span>The result showed that an increase in heart MDA and DNA fragmentation<span><span><span> levels were detected while significant decreases were seen in SOD<span> levels in CP alone group when compared to the other groups. CP caused severe damage in the histopathological status of heart tissue including intersititial oedema, haemorrhage, degeneration and necrosis in muscle fibrils and perinuclear </span></span>vacuolization. A significant increase in the percentage of TUNEL-positive cells and γH2AX </span>protein expression was detected in the CP-treated group compared to the control and other treated groups. There was significant increase in the percentage of caspase 3-positive cells and decrease in the percentage of Bcl-2 positive cells in the CP group compared to the control group and other treated groups. However, a significant decrease in the percentage of cTnI and cTnT </span></span>immunoreactivity was also observed in the CP-treated group compared to the control and other treated groups. In the groups in which SLY and CUR were administered concurrently with CP, biochemical parameters, histopathological and immunohistochemical results were found to be significantly lower than in the CP-only group.</p></div><div><h3>Conclusions</h3><p>These results lead to conclusion that the natural antioxidant<span> SLY and CUR might have protective effects against CP-induced cardiotoxicity and oxidative stress in rats.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 317-327"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34763184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on brush border membrane enzymes, carbohydrate metabolism and antioxidant system in rat intestine","authors":"Faaiza Shahid ,&nbsp;Zeba Farooqui ,&nbsp;Sana Rizwan ,&nbsp;Subuhi Abidi ,&nbsp;Iqbal Parwez ,&nbsp;Farah Khan","doi":"10.1016/j.etp.2017.02.001","DOIUrl":"https://doi.org/10.1016/j.etp.2017.02.001","url":null,"abstract":"<div><p><span><span>Cisplatin (CP) is an effective chemotherapeutic agent that induces </span>gastrointestinal toxicity. </span><span><em>Nigella sativa</em></span><span> oil (NSO) has been shown to be beneficial in a wide range of gastrointestinal disorders. The present study investigates the possible protective effect of NSO on CP-induced gastrointestinal toxicity. NSO administration (2</span> <span>ml/kg bwt, orally), prior to and following, a single dose CP treatment (6</span> <span><span>mg/kg bwt. ip), significantly attenuated the CP-induced decrease in brush border membrane (BBM) enzyme activities in intestinal homogenates and BBM vesicles<span> (BBMV). NSO administration also mitigated CP induced alterations in the activities of carbohydrate metabolism enzymes and in the enzymatic and non-enzymatic antioxidant parameters in the intestine. The results suggest that NSO by empowering the endogenous antioxidant system improves intestinal redox and metabolic status and restores BBM integrity in CP treated rats. Histopathological studies supported the biochemical findings. Thus, NSO may help prevent the accompanying </span></span>gastrointestinal dysfunction in CP chemotherapy.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 299-306"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71830060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytogenotoxicological defense of retinyl palmitate in the front damage of antineoplastics.","authors":"Ricardo Melo de Carvalho, R. P. Aguiar, M. T. Islam, M. A. D. de Alencar, Ana Maria oliveira da Mata, A. L. Braga, Josemar José da Silva Júnior, L. R. Sousa, Rosália Maria Tôrres de Lima, M. F. C. J. Paz, J. M. C. E. E Sousa, A. A. Melo-Cavalcante","doi":"10.1016/j.etp.2017.01.013","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.013","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"34 1","pages":"293-297"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81520200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential protective role of taurine against 5-fluorouracil-induced nephrotoxicity in adult male rats.","authors":"H. Yousef, H. Aboelwafa","doi":"10.1016/j.etp.2017.01.012","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.012","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"43 1","pages":"265-274"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86937004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on brush border membrane enzymes, carbohydrate metabolism and antioxidant system in rat intestine.","authors":"F. Shahid, Z. Farooqui, S. Rizwan, S. Abidi, I. Parwez, F. Khan","doi":"10.1016/j.etp.2017.02.001","DOIUrl":"https://doi.org/10.1016/j.etp.2017.02.001","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"6 1","pages":"299-306"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78472136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progression of renal fibrosis in congenital CKD model rats with reduced number of nephrons","authors":"Hidenori Yasuda,&nbsp;Yuki Tochigi,&nbsp;Kentaro Katayama,&nbsp;Hiroetsu Suzuki","doi":"10.1016/j.etp.2017.01.007","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.007","url":null,"abstract":"<div><p><span><span>A congenital reduction in the number of nephrons is a critical risk factor for both onset of chronic </span>kidney disease (CKD) and its progression to end-stage kidney disease (ESKD). Hypoplastic kidney (HPK) rats have only about 20% of the normal number of nephrons and show progressive CKD. This study used an immunohistological method to assess glomerular and interstitial pathogenesis in male HPK rats aged 35–210</span> <span>days. CD68<span> positive-macrophages were found to infiltrate into glomeruli in HPK rats aged 35 and 70</span></span> <!-->days and to infiltrate into interstitial tissue in rats aged 140 and 210<!--> <!-->days. HPK rats aged 35 and 70<!--> <span><span><span>days showed glomerular hypertrophy, loss of normal linear </span>immunostaining of podocine, and increased expression of PDGFr-β, TGF-β, collagens, and </span>fibronectin<span>, with all of these alterations gradually deteriorating with age. α-SMA-positive myofibroblasts were rarely detected in glomerular tufts, whereas α-SMA-positive glomerular parietal epithelium (GPE) cells were frequently observed along Bowman’s capsular walls. The numbers of PDGFr-β-positive fibroblasts in interstitial tissue were increased in rats aged 35</span></span> <span>days and older, whereas interstitial fibrosis<span>, characterized by the increased expression of tubular PDGF-BB, the appearance of myofibroblasts doubly positive for PDGFr-β and α-SMA, and increased expression of collagens and fibronectin, were observed in rats aged 70 and older. These results clearly indicate that congenital CKD with only 20% of nephrons cause renal fibrosis in rats.</span></span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 245-258"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.01.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71830055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A spontaneous high-grade undifferentiated mammary carcinoma in a seven-week-old female rat","authors":"Ana I. Faustino-Rocha ,&nbsp;Adelina Gama ,&nbsp;Paula A. Oliveira ,&nbsp;Antonieta Alvarado ,&nbsp;Rita Ferreira ,&nbsp;Mário Ginja","doi":"10.1016/j.etp.2017.01.006","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.006","url":null,"abstract":"<div><p><span>The present work describes a rare case of a spontaneous high-grade carcinoma in a seven-week-old Sprague-Dawley female rat that had been included in the control group of an assay of mammary carcinogenesis. The mass was detected at 50</span> <span>days of age, it grown quickly and the animal was humanely sacrificed eight days later. The tumor was located in the left cervical region, in the vicinity of the left submandibular and sublingual glands<span>. It was soft and reddish and had several dens with a bloody content. The tumor was PAS negative and exhibited immunostaining for ER-α. The histopathologic and immunohistochemical data are suggestive of a high-grade carcinoma from mammary gland. It was the first report of a spontaneous mammary tumor in such a young rat.</span></span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 241-244"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.01.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71830057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metaplastic phenotype in human primary bronchiolar epithelial cells after repeated exposure to native mainstream smoke at the air-liquid interface","authors":"Michaela Aufderheide ,&nbsp;Shigeaki Ito ,&nbsp;Shinkichi Ishikawa ,&nbsp;Makito Emura","doi":"10.1016/j.etp.2017.01.015","DOIUrl":"10.1016/j.etp.2017.01.015","url":null,"abstract":"<div><p><span>3D constructs composed of primary normal differentiated human bronchiolar epithelial (NHBE) cells as mono- or co-culture in combination with normal human lung fibroblasts were exposed repeatedly at the air-liquid interface with non-lethal concentrations of mainstream cigarette smoke (4 cigarettes a day, 5</span> <span>days/week, 13 times repetition in total) to build up a permanent burden on the cells. Samples were taken after 4, 8 and 13 times of repeated smoke exposure and the cultures were analyzed by histopathological methods In comparison with the clean air exposure (process control) and incubator control cells the cigarette smoke exposed cultures showed a reduction of cilia bearing as well as mucus<span> producing cells. In both mono- as well as co-cultures, hyperplasia<span><span><span> was induced showing different histological cell types (undifferentiated secretory and squamous cell types). At the end of the exposure phase, we observed the development of non-hyperplastic areas strongly positive to CK13 antibody, commonly seen in squamous cells as a marker for non-cornified </span>squamous epithelium, thus suggesting a transition of the normal bronchial epithelial cells towards </span>metaplastic cells. The control cultures (clean air exposed and incubator cells) showed no comparable phenotypic changes. In conclusion, our </span></span></span><em>in vitro</em> model presents a valuable tool to study the induction of metaplastic alterations after exposure to airborne material.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 307-315"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.01.015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34777401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological and biochemical evaluation of coumarin and chlorophyllin against aflatoxicosis in rat.","authors":"M. S. Abdel-Latif, K. Elmeleigy, T. Aly, M. Khattab, S. M. Mohamed","doi":"10.1016/j.etp.2017.01.014","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.014","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"24 1","pages":"285-291"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84357847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}