Experimental and Toxicologic Pathology最新文献

{"title":"editorial board (IFC)","authors":"","doi":"10.1016/S0940-2993(17)30314-7","DOIUrl":"https://doi.org/10.1016/S0940-2993(17)30314-7","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 6","pages":"Page IFC"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0940-2993(17)30314-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71862408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity and genotoxicity effects of arsenic trioxide on SQ20B human laryngeal carcinoma cells","authors":"Fatma Trabelsi ,&nbsp;Rim Khlifi ,&nbsp;Didier Goux ,&nbsp;Marilyne Guillamin ,&nbsp;Amel Hamza-Chaffai ,&nbsp;François Sichel","doi":"10.1016/j.etp.2017.02.003","DOIUrl":"10.1016/j.etp.2017.02.003","url":null,"abstract":"<div><p><span><span>This study investigates the cytotoxicity and the genotoxicity induced by </span>arsenic trioxide As</span>₂O₃<span>in human laryngeal SQ20B carcinoma cell line. SQ20B cells were exposed to graded concentrations of arsenic trioxide (2 and 5</span> <!-->μM) for 48<!--> <span>h. Comet assay<span> and γ-H2AX foci formation were used for measuring DNA damages, flow cytometry was used to identify cell cycle alterations and apoptosis<span>, while cell morphology was visualized using transmission electron microscopy. The results show a dose-dependent induction of DNA damages and double strand breaks, alterations in cell cycle and morphologic alterations of cells. These results prove that As</span></span></span>₂O₃<span> is highly cytotoxic and genotoxic at the micromolar range ina human laryngeal carcinoma cell line.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 6","pages":"Pages 349-358"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34784964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The exposure to formaldehyde causes renal dysfunction, inflammation and redox imbalance in rats","authors":"Camila de Oliveira Ramos ,&nbsp;Clarissa Rodrigues Nardeli ,&nbsp;Keila Karine Duarte Campos ,&nbsp;Karina Braga Pena ,&nbsp;Dafne Fernandes Machado ,&nbsp;Ana Carla Balthar Bandeira ,&nbsp;Guilherme de Paula Costa ,&nbsp;André Talvani ,&nbsp;Frank Silva Bezerra","doi":"10.1016/j.etp.2017.02.008","DOIUrl":"10.1016/j.etp.2017.02.008","url":null,"abstract":"<div><p><span>Twenty-eight Fischer male rats were divided into four groups: control group (CG), exposed to the ambient air, and groups exposed to formaldehyde (FA) at concentrations of 1% (FA1%), 5% (FA5%) and 10% (FA10%). Kidney function<span><span> was assessed by dosage of uric acid, creatinine and urea. </span>Morphometry was performed on the thickness of the lumen of </span></span>Bowman's capsule<span><span> and diameter of the lumen of the renal tubules<span><span>. We evaluated the redox imbalance through the catalase<span> and superoxide dismutase activity as well as oxidative damage by </span></span>lipid peroxidation. Inflammatory chemokines CCL2, CCL3 and </span></span>CCL5 were analyzed by enzyme immunoassays. There was an increase in the concentration of urea in FA10% compared with CG and FA1%. The levels of creatinine, renal lumen and lipid peroxidation increased in all FA-treated groups compared with CG. The concentration of uric acid in FA10% was lower compared with all other groups. There was an increase in the space of Bowman's capsule in FA5% and FA10% compared with CG and FA1%. However, the superoxide dismutase activity was higher in FA5% compared with other groups while CCL5 was higher in FA1% compared with CG. The exposure to formaldehyde in a short period of time leads to changes in the kidney function, inflammation and morphology, as well as promoted the increase of superoxide dismutase activity and oxidative damage.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 6","pages":"Pages 367-372"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34849969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcuma longa Linn. extract and curcumin protect CYP 2E1 enzymatic activity against mercuric chloride-induced hepatotoxicity and oxidative stress: A protective approach","authors":"Deepmala Joshi ,&nbsp;Deepak Kumar Mittal ,&nbsp;Sangeeta Shukla ,&nbsp;Sunil Kumar Srivastav ,&nbsp;Vaibhav A. Dixit","doi":"10.1016/j.etp.2017.02.009","DOIUrl":"10.1016/j.etp.2017.02.009","url":null,"abstract":"<div><p>The present investigation has been conducted to evaluate the therapeutic potential of <span><em>Curcuma longa</em></span> (200<!--> <!-->mg<!--> <!-->kg⁻¹<span>, po) and curcumin (80</span> <!-->mg<!--> <!-->kg⁻¹, po) for their hepatoprotective efficacy against mercuric chloride (HgCl₂: 12<!--> <!-->μmol<!--> <!-->kg⁻¹<span>, ip; once only) hepatotoxicity. The HgCl</span>₂<span><span><span><span> administration altered various biochemical parameters, including transaminases, </span>alkaline phosphatase, </span>lactate dehydrogenase, </span>bilirubin<span>, gamma-glutamyl transferase, triglycerides and cholesterol contents with a concomitant decline in protein and albumin concentration in serum which were restored towards control by therapy of </span></span><em>Curcuma longa</em><span> or curcumin. On the other hand, both treatments<span> showed a protective effect on drug metabolizing enzymes </span></span><em>viz.</em><span><span><span> aniline hydroxylase<span> (AH) and amidopyrine-N-demethylase (AND), hexobarbitone induced sleep time and </span></span>BSP retention. </span>Choleretic, 1,1-diphenyl-2-picryl-hydrazil (DPPH)-free radical scavenging activities and histological studies also supported the biochemical findings. The present study concludes that </span><em>Curcuma longa</em> extract or curcumin has the ability to alleviate the hepatotoxic effects caused by HgCl₂ in rats.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 6","pages":"Pages 373-382"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34850600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Artesunate on some biochemical parameters in pregnant albino Wistar rats challenged with lethal strain Plasmodium berghei NK65: Appreciating the activities of artemisinin drugs on key pregnancy hormone balance","authors":"Chinaka O. Nwaehujor ,&nbsp;Onyeka V. Asuzu ,&nbsp;Daniel D. Nwibo ,&nbsp;Obichukwu C. Nwobi ,&nbsp;Ihechiluru I. Ezeigbo","doi":"10.1016/j.etp.2017.02.005","DOIUrl":"10.1016/j.etp.2017.02.005","url":null,"abstract":"<div><p><span><span>In humans, malaria in pregnancy can cause serious maternal and foetal morbidity and in extreme untreated cases, foetal mortality occurs. The therapeutic approach to curbing this malaise is the administration of an effective and/or combinations of anti-malaria medicaments. Acute or chronic administration of some of these </span>drugs<span>, however, gives rise to some adverse medical conditions including reproductive dysfunction, especially in pregnancy. Studies aimed at the hormonal interplays following administration of these drugs in pregnancy have been limited due to too few appropriate animal models. In this experiment, pregnant albino<span> rats were infected with rodent parasite, </span></span></span><span><em>Plasmodium berghei</em></span> on the 5th day of gestation, following which biochemical changes, specific for pregnancy maintenance were monitored in the blood of test rats. We observed that infecting the pregnant rats with <em>P. berghei</em><span> negatively impacted the measured biological parameters (hormones) compared to unchallenged controls. The observed effect was however retreated following oral administration of 3</span> <span><span>mg/kg body weight, qDay of Artesunate until the 17th day of gestation. Findings, therefore, suggest that Artesunate is an effective therapeutic agent in pregnancy, demonstrated by the restoration of the hormonal changes occasioned by the </span>parasitic infection.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 6","pages":"Pages 408-411"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34850601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypical changes in a differentiating immortalized bronchial epithelial cell line after exposure to mainstream cigarette smoke and e-cigarette vapor","authors":"Michaela Aufderheide,&nbsp;Makito Emura","doi":"10.1016/j.etp.2017.03.004","DOIUrl":"10.1016/j.etp.2017.03.004","url":null,"abstract":"<div><p>3D constructs composed of differentiated immortalized primary normal human bronchial epithelial (NHBE) cells (CL-1548) were repeatedly exposed at the air-liquid interface to non-lethal concentrations of mainstream cigarette smoke (4 cigarettes a day, 5<!--> <span>days/week, 8 repetitions in total) and e-cigarette vapor (50 puffs a day, 5 days/week, 8 repetitions in total) to build up a permanent burden on the cells. Samples were taken after 4, 6 and 8 times of repeated smoke exposure and the cultures were investigated using histopathological methods Compared to the clean air-exposed cultures (process control) and incubator control, the aerosol-exposed cultures showed a reduction of ciliated, mucus-producing and club cells. At the end of the exposure phase, we even found metaplastic areas positive for CK13<span> antibody in the cultures exposed to mainstream cigarette smoke and e-liquid vapor, commonly seen in squamous cells<span> as a marker for non-cornified squamous epithelium. The control cultures (incubator cells) showed no comparable phenotypical changes. In conclusion, our </span></span></span><em>in vitro</em> model presents a valuable tool to study the induction of phenotypical changes after exposure to hazardous airborne material.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 6","pages":"Pages 393-401"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.03.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34881221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of the CULTEX® exposure technology by radial distribution of the test aerosol","authors":"Michaela Aufderheide ,&nbsp;Wolf-Dieter Heller ,&nbsp;Olaf Krischenowski ,&nbsp;Niklas Möhle ,&nbsp;Dieter Hochrainer","doi":"10.1016/j.etp.2017.02.004","DOIUrl":"https://doi.org/10.1016/j.etp.2017.02.004","url":null,"abstract":"<div><p>The exposure of cellular based systems cultivated on microporous membranes at the air-liquid interface (ALI) has been accepted as an appropriate approach to simulate the exposure of cells of the respiratory tract to native airborne substances. The efficiency of such an exposure procedure with regard to stability and reproducibility depends on the optimal design at the interface between the cellular test system and the exposure technique. The actual exposure systems favor the dynamic guidance of the airborne substances to the surface of the cells in specially designed exposure devices. Two module types, based on a linear or radial feed of the test atmosphere to the test system, were used for these studies. In our technical history, the development started with the linear designed version, the CULTEX<strong>^®</strong> glass modules, fulfilling basic requirements for running ALI exposure studies (Mohr and Durst, 2005). The instability in the distribution of different atmospheres to the cells caused us to create a new exposure module, characterized by a stable and reproducible radial guidance of the aerosol to the cells. The outcome was the CULTEX<strong>^®</strong> RFS (Mohr et al., 2010). In this study, we describe the differences between the two systems with regard to particle distribution and deposition clarifying the advantages and disadvantages of a radial to a linear aerosol distribution concept.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 6","pages":"Pages 359-365"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71862383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal exposure to titanium dioxide nanoparticles during pregnancy and lactation alters offspring hippocampal mRNA BAX and Bcl-2 levels, induces apoptosis and decreases neurogenesis","authors":"Alireza Ebrahimzadeh Bideskan ,&nbsp;Abbas Mohammadipour ,&nbsp;Alireza Fazel ,&nbsp;Hossein Haghir ,&nbsp;Houshang Rafatpanah ,&nbsp;Mahmoud Hosseini ,&nbsp;Aliakbar Rajabzadeh","doi":"10.1016/j.etp.2017.02.006","DOIUrl":"10.1016/j.etp.2017.02.006","url":null,"abstract":"<div><h3>Introduction</h3><p><span>The usage of Titanium dioxide nanoparticles (TiO</span>₂<span>-NPs) covers a vast area in different fields ranging from cosmetics and food to the production of drugs. Maternal exposure to TiO</span>₂-NPs during developmental period has been associated with hippocampal injury and with a decrease in learning and memory status of the offspring. However, little is known about its injury mechanism. This paper describes the in vivo neurotoxic effects of TiO₂<span>-NPs on rat offspring hippocampus during developmental period.</span></p></div><div><h3>Material and methods</h3><p><span>Pregnant and lactating Wistar rats received intragastric TiO</span>₂-NPs (100<!--> <!-->mg/kg body weight) daily from gestational day (GD) 2 to (GD) 21 and postnatal day (PD) 2 to (PD) 21 respectively. Animals in the control groups received an equal volume of distilled water via gavage.</p><p><span><span>At the end of the treatment<span> process, offspring were deeply anesthetized and sacrificed. Then brains of each group were collected and sections of the rat offspring’s brains were stained using </span></span>TUNEL staining<span> (for detection of apoptotic cells) and immunostaining (for neurogenesis). Moreover, the right hippocampus (n</span></span> <!-->=<!--> <span>6 per each group) were removed from the right hemisphere for evaluating the expression of Bax and Bcl-2 level.</span></p></div><div><h3>Results</h3><p>Results of histopatological examination by TUNEL staining showed that maternal exposure to TiO₂<span>-NPs during pregnancy and lactation periods increased apoptotic cells significantly (P</span> <!-->&lt;<!--> <span><span>0.01) in the offspring hippocampus. The immunolabeling of double cortin (DCX) protein as </span>neurogenesis marker also showed that TiO</span>₂-NPs reduced neurogenesis in the hippocampus of the offspring (P<!--> <!-->&lt;<!--> <!-->0.05). Moreover, in comparison with the control group, the mRNA levels of Bax and Bcl-2 in the TiO₂-NPs group significantly increased and decreased, respectively (P<!--> <!-->&lt;<!--> <!-->0.01).</p></div><div><h3>Conclusion</h3><p>These findings provide strong evidence that maternal exposure to TiO₂<span>-NPs significantly impact hippocampal neurogenesis and apoptosis in the offspring. The potential impact of nanoparticle exposure for millions of pregnant mothers and their offspring across the world is potentially devastating.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 6","pages":"Pages 329-337"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34777833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfated glycosaminoglycans in bladder tissue and urine of rats after acute exposure to paraoxon and cyclophosphamide","authors":"Vladislav E. Sobolev ,&nbsp;Richard O. Jenkins ,&nbsp;Nikolay V. Goncharov","doi":"10.1016/j.etp.2017.02.007","DOIUrl":"10.1016/j.etp.2017.02.007","url":null,"abstract":"<div><p><span><span><span>Glycosaminoglycans (GAGs) in the urine of </span>Wistar rats<span><span>, and on the surface of the epithelium and lamina propria of the </span>bladder<span>, were quantitatively assessed before and after acute intoxication with paraoxon or </span></span></span>cyclophosphamide. Paraoxon was administered subcutaneously (s.c.) twice with an interval of 1</span> <!-->h to a final dose of 275<!--> <!-->mg/kg; cyclophosphamide was administered intraperitoneally (i.p.) with a single dose of 100<!--> <!-->mg/kg or to a final dose of 240<!--> <!-->mg/kg (three times per 80<!--> <!-->mg/kg every 12<!--> <span>h). GAGs on the surface of the epithelium and lamina propria of the urinary<span><span> bladder of rats were quantitatively determined by Alcian blue dye staining. GAGs in the urine were determined spectrophotometrically with 1-9-dimethyl </span>methylene blue. By 48–96</span></span> <span><span>h after intoxication with either paraoxon or cyclophosphamide, statistically significant increases in the concentration of GAGs were obtained both for the tissues of the bladder and the urine of rats. Cyclophosphamide, in contrast to paraoxon, caused the development of hemorrhagic cystitis in the animals. The synthesis of GAGs is considered to be compensatory response to the toxic </span>xenobiotics.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 6","pages":"Pages 339-347"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34782667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of silymarin and curcumin on cyclophosphamide-induced cardiotoxicity","authors":"H. Avci ,&nbsp;E.T. Epikmen ,&nbsp;E. Ipek ,&nbsp;R. Tunca ,&nbsp;S.S. Birincioglu ,&nbsp;H. Akşit ,&nbsp;S. Sekkin ,&nbsp;A.N. Akkoç ,&nbsp;M. Boyacioglu","doi":"10.1016/j.etp.2017.02.002","DOIUrl":"10.1016/j.etp.2017.02.002","url":null,"abstract":"<div><h3>Introduction</h3><p>Cyclophosphamide<span><span> (CP) is a potent anticancer agent; its clinical use is limited due to its marked </span>cardiotoxicity.</span></p></div><div><h3>Aim</h3><p><span>The present study was aimed at evaluating the cardioprotective effects of </span>silymarin<span> (SLY) and curcumin (CUR), which have strong antioxidant properties, against the toxic effects of high-dose CP on the heart of rats.</span></p></div><div><h3>Materials and methods</h3><p><span>A total of 36 adult Wistar albino female rats were randomly divided into six groups. Group I (control group; nothing was administered), Group II (CP group; 30</span> <!-->mg/kg/day CP was administered intraperitoneally to each animal for seven days), Group III (SLY group; 100<!--> <!-->mg/kg/day SLY by gavage for 14 days), Group IV (CUR group; 100<!--> <!-->mg/kg/day CUR by gavage for 14 days), Group V (SLY<!--> <!-->+<!--> <!-->CP group; 100<!--> <!-->mg/kg/day SLY by gavage for 14<!--> <!-->days plus 30<!--> <!-->mg/kg/day CP intraperitoneally starting from the seventh day) and Group VI (CUR<!--> <!-->+<!--> <!-->CP group; 100<!--> <!-->mg/kg/day CUR by gavage for 14<!--> <!-->days plus 30<!--> <!-->mg/kg/day CP intraperitoneally starting from the seventh day). Biochemical, histopathological and immunohistochemical methods were utilised for evaluation of the cardiotoxicity.</p></div><div><h3>Results</h3><p><span>The result showed that an increase in heart MDA and DNA fragmentation<span><span><span> levels were detected while significant decreases were seen in SOD<span> levels in CP alone group when compared to the other groups. CP caused severe damage in the histopathological status of heart tissue including intersititial oedema, haemorrhage, degeneration and necrosis in muscle fibrils and perinuclear </span></span>vacuolization. A significant increase in the percentage of TUNEL-positive cells and γH2AX </span>protein expression was detected in the CP-treated group compared to the control and other treated groups. There was significant increase in the percentage of caspase 3-positive cells and decrease in the percentage of Bcl-2 positive cells in the CP group compared to the control group and other treated groups. However, a significant decrease in the percentage of cTnI and cTnT </span></span>immunoreactivity was also observed in the CP-treated group compared to the control and other treated groups. In the groups in which SLY and CUR were administered concurrently with CP, biochemical parameters, histopathological and immunohistochemical results were found to be significantly lower than in the CP-only group.</p></div><div><h3>Conclusions</h3><p>These results lead to conclusion that the natural antioxidant<span> SLY and CUR might have protective effects against CP-induced cardiotoxicity and oxidative stress in rats.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 317-327"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34763184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}