Cytotoxicity and genotoxicity effects of arsenic trioxide on SQ20B human laryngeal carcinoma cells

Q2 Medicine

Experimental and Toxicologic Pathology Pub Date : 2017-07-05 DOI:10.1016/j.etp.2017.02.003

Fatma Trabelsi , Rim Khlifi , Didier Goux , Marilyne Guillamin , Amel Hamza-Chaffai , François Sichel

引用次数: 1

Abstract

This study investigates the cytotoxicity and the genotoxicity induced by arsenic trioxide As₂O₃in human laryngeal SQ20B carcinoma cell line. SQ20B cells were exposed to graded concentrations of arsenic trioxide (2 and 5 μM) for 48 h. Comet assay and γ-H2AX foci formation were used for measuring DNA damages, flow cytometry was used to identify cell cycle alterations and apoptosis, while cell morphology was visualized using transmission electron microscopy. The results show a dose-dependent induction of DNA damages and double strand breaks, alterations in cell cycle and morphologic alterations of cells. These results prove that As₂O₃ is highly cytotoxic and genotoxic at the micromolar range ina human laryngeal carcinoma cell line.

查看原文本刊更多论文

三氧化二砷对人喉癌SQ20B细胞的细胞毒性和遗传毒性作用

研究了三氧化二砷as2o3对人喉癌SQ20B细胞的细胞毒性和遗传毒性。将SQ20B细胞暴露于浓度不同的三氧化二砷(2 μM和5 μM)中48 h，采用彗星法和γ-H2AX聚焦法检测DNA损伤，流式细胞术检测细胞周期变化和凋亡，透射电镜观察细胞形态。结果显示，剂量依赖性诱导DNA损伤和双链断裂，细胞周期改变和细胞形态改变。这些结果证明，As2O3在人喉癌细胞系的微摩尔范围内具有高度的细胞毒性和遗传毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Experimental and Toxicologic Pathology 医学-病理学

CiteScore

2.08

自引率

0.00%

发文量

审稿时长

5.3 weeks

期刊介绍： Cessation. The international multidisciplinary journal is devoted to the publication of studies covering the whole range of experimental research on disease processes and toxicology including cell biological investigations. Its aim is to support progress in the interdisciplinary cooperation of researchers working in pathobiology, toxicology, and cell biology independent of the methods applied. During the past decades increasing attention has been paid to the importance of toxic influence in the pathogenesis of human and animal diseases. This is why Experimental and Toxicologic Pathology meets the urgent need for an interdisciplinary journal felt by a wide variety of experts in medicine and biology, including pathologists, toxicologists, biologists, physicians, veterinary surgeons, pharmacists, and pharmacologists working in academic, industrial or clinical institutions.