Experimental and Toxicologic Pathology最新文献

{"title":"Comparison of the effects of various lubricant eye drops on the in vitro rabbit corneal healing and toxicity.","authors":"R. Dutescu, C. Panfil, N. Schrage","doi":"10.1016/j.etp.2016.12.002","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.002","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"31 1","pages":"123-129"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90070921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential risks of maternal administration of Mucophylline on the pups of albino rats during lactation.","authors":"H. Hamdi, Heba M. Ali","doi":"10.1016/j.etp.2016.12.008","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.008","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"87 1","pages":"143-152"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84205410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential risks of maternal administration of Mucophylline on the pups of albino rats during lactation","authors":"Hamida Hamdi ,&nbsp;Heba Ali","doi":"10.1016/j.etp.2016.12.008","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.008","url":null,"abstract":"<div><p><span><span>The present study was undertaken to evaluate the potential risks of the mucolytic and broncholytic </span>drug<span>, Theophylline derivatives (Mucophylline) maternally administered on the pups. The nursing rats orally administered from 1st postpartum day (PPD) to 21th PPD with two different doses 30.83</span></span> <!-->mg/kg (low dose) and 66.61<!--> <span><span>mg/kg (Human equivalent dose (HED)). On the 21th PPD, the postnatal developmental signs, skeletal malformation and the </span>histopathology<span> of neonatal liver, kidney<span><span> and brain<span> were examined. Our results showed that Mucophylline induced a significant reduction in the neonatal weight and length, delayed, weak and incomplete ossification, wavy ribs and the neonatal liver revealed histopathological changes, pyknotic hepatocytes, cytoplasmic vacuolization, dilated </span></span>sinusoid<span><span><span> and necrotic area. Kidney revealed alternation changes, enlargement of the glomerulus, </span>renal tubules degeneration and lymphatic </span>infiltration<span>. Brain (cerebral cortex and cerebellum) showed neurodegenerative changes, vacuolization of neuropil, congested and dilated blood vessel and dark stain neurons. Our results showed that the activities of non-enzymatic (GSH) and enzymatic (GST, CAT) antioxidants were insignificantly decrease in both neonatal brain and liver tissues of rats administered with 30.83</span></span></span></span></span> <!-->mg/kg and 61.66<!--> <span>mg/kg of Mucophylline and insignificant increase in MDA levels in both neonatal brain and liver tissues. However, significant reduction (P</span> <!-->≤<!--> <span>0.05) in the content of GR was recorded in neonatal brain tissue of rats administered with 30.83</span> <!-->mg/kg and 61.66<!--> <span>mg/kg of Mucophylline during lactation period in comparison with control. These support and proof the potential risks of the maternal administration of Mucophylline on pups.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 3","pages":"Pages 143-152"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2016.12.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71813904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of Tritone (Livosone) on oxidative DNA damage and its hepatoprotective potential against various hepatotoxic agent in wistar rats","authors":"Sheetal Kashinath Medhekar ,&nbsp;Tejas Pandurang Jadhav ,&nbsp;Vishal Sadashiv Sasane ,&nbsp;Vikas Suresh Shende ,&nbsp;Nagesh Hanmantrao Aloorkar ,&nbsp;Anjali Baburao Chincholkar ,&nbsp;Girish Sudhakar Soman ,&nbsp;Ajit Shankarrao Kulkarni","doi":"10.1016/j.etp.2016.12.005","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.005","url":null,"abstract":"<div><h3>Aim</h3><p>To evaluate antioxidant activity, DNA damage inhibition and hepatoprotecitve potential of polyherbal formulation Tritone (Livosone).</p></div><div><h3>Methods</h3><p><em>In vitro</em><span><span> antioxidant activity of Tritone formulation was performed by using DPPH assay. Hepatoprotecitve potential of Tritone was evaluated against various </span>hepatotoxic<span> agents including Paracetamol (2</span></span> <span>g/kg b. wt p.o. single dose on 15th day), Galactosamine (400</span> <!-->mg/kg b. wt. i.p. single dose on 8th day) and Alcohol (30% p.o.1<!--> <!-->ml/100<!--> <!-->g of rat for 15<!--> <!-->days). Tritone formulation at the doses of (40.5, 81 and 162<!--> <span>mg/kg) and standard silymarin (100</span> <!-->mg/kg) and Liv52 (270<!--> <span><span>mg/kg) were administered p.o. The hepatoprotective assessment was done by estimating biochemical parameters: SGOT<span><span>, SGPT, ALP and Total </span>Bilirubin<span> total protein and ChE levels. Additionally histopathological and </span></span></span>DNA fragmentation study of Tritone was also performed.</span></p></div><div><h3>Result</h3><p><span><span><span>Administration of hepatotoxins (paracetamol, D-GaiN and alcohol) in </span>experimental animals showed significant biochemical, histological deterioration and DNA fragmentation. </span>Pretreatment with Tritone (Livosone) shows significant reduction in serum SGOT, SGPT, ALP and total bilirubin levels and shows significant elevation in total protein and cholinesterase (ChE) levels compared to groups treated with hepatotoxic agents. Histopathological observations of rat liver pretreated with Tritone (Livosone) shows significant protection against hepatic damage. Inhibition of DNA fragmentation by Tritone indicates protective effect of formulation on liver at molecular level. Finally all the results were compared with standard </span>drugs Silymarin and Liv52.</p></div><div><h3>Conclusion</h3><p><span>Correlation of antioxidant activity, biochemical results, histopathological changes and inhibition of DNA damage after treatment with Tritone shows maximum hepatoprotective potential at dose 81</span> <!-->mg/kg and 162<!--> <!-->mg/kg.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 3","pages":"Pages 153-161"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2016.12.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71814433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological evaluation of a new pulp capping material developed from Portland cement.","authors":"A. Negm, E. Hassanien, A. Abu-Seida, M. Nagy","doi":"10.1016/j.etp.2016.12.006","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.006","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"66 1","pages":"115-122"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83927547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extract of bulbus Fritillaria cirrhosa perturbs spindle assembly checkpoint, induces mitotic aberrations and genomic instability in human colon epithelial cell line.","authors":"Xihan Guo, J. Ni, Jing-lun Xue, Xu Wang","doi":"10.1016/j.etp.2016.12.009","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.009","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"17 1","pages":"163-171"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90891637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological evaluation of a new pulp capping material developed from Portland cement","authors":"Ahmed M. Negm ,&nbsp;Ehab E. Hassanien ,&nbsp;Ashraf M. Abu-Seida ,&nbsp;Mohamed M. Nagy","doi":"10.1016/j.etp.2016.12.006","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.006","url":null,"abstract":"<div><p><span>This study evaluates the biological properties of a new pulp capping material developed from Portland cement. This study was conducted on 48 teeth in 4 dogs (12 teeth/dog). The dogs were classified into two equal groups (n</span> <!-->=<!--> <!-->24 teeth) according to the evaluation period including: group A (3 weeks) and group B (3 months). Each group was further subdivided into three equal subgroups (n<!--> <!-->=<!--> <span>8 teeth) according to the capping material including: subgroup 1: mineral trioxide aggregate (MTA), subgroup2: Portland cement</span> <!-->+<!--> <span>10% calcium hydroxide</span> <!-->+<!--> <!-->20% bismuth oxide (Port Cal) and subgroup 3: Portland cement<!--> <!-->+<!--> <span><span><span>bismuth oxide. After general anesthesia<span>, a class V buccal cavity was prepared coronal to the gingival margin<span>. After pulp exposure and hemostasis,the capping materials and </span></span></span>glass ionomer filling were placed on the exposure sites. All histopathological findings, </span>inflammatory cell<span> count and dentin bridge formation were recorded. Data were analyzed statistically. After 3 months, the histopathological picture of the pulp in subgroup 1 showed normal pulp, continuous odontoblastic layer and complete dentin bridge formation while subgroup 2 showed partial and complete dentin bridge over a normal and necrotic pulps. Subgroup 3 showed loss of normal architecture, areas of necrosis, complete, or incomplete dentin bridge formation, attached and detached pulp stones and fatty degeneration in group B. For group A, MTA subgroup showed the least number of inflammatory cell infiltrate followed by Port Cal subgroup. While subgroup 3 showed the highest number of inflammatory cell infiltrate. For group B, the mean inflammatory cell count increased with the three tested materials with no statistical difference. Regarding dentin bridge formation at group A, no significant differences was found between subgroups, while at group B, MTA subgroup exhibited significantly higher scores than other subgroups. In conclusion, addition of calcium hydroxide to Portland cement improves the dentin bridge formation qualitatively and quantitatively.</span></span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 3","pages":"Pages 115-122"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2016.12.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71813905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the effects of various lubricant eye drops on the in vitro rabbit corneal healing and toxicity","authors":"R. Michael Dutescu,&nbsp;Claudia Panfil,&nbsp;Norbert Schrage","doi":"10.1016/j.etp.2016.12.002","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.002","url":null,"abstract":"<div><p><span>Ingredients of lubricant eye drops are potentially harmful to the ocular surface<span>. The products Optive, Optive Fusion, Neopt<span> were tested regarding corneal irritability versus Vismed Multi and 0.01% benzalkonium chloride as negative and positive control, respectively. Formulas (30–40</span></span></span> <!-->μl per hour) were applied hourly in-vitro for six days on rabbit corneas (n<!--> <!-->=<!--> <span><span>5, per product) cultured in artificial anterior chambers (EVEIT system). Initially, four </span>corneal abrasions (2.4–4.6</span> <!-->mm²<span><span>) were induced. All defects were monitored during drop application by fluorescein stains and </span>photographs. To ensure corneal vitality, glucose and lactate concentrations in artificial anterior chamber fluids were determined photometrically.</span></p><p>All products showed a complete corneal healing on day 2. Thereafter, all five Optive-treated corneas developed progressive fluorescein-positive epithelial lesions until day six (24.96<!--> <!-->μm, ±21.45<!--> <!-->μm, p<!--> <!-->&lt;<!--> <span>0.01). For Optive Fusion three corneas showed corneal erosions on day six (23.11</span> <!-->μm, ±37.02<!--> <!-->μm, p<!--> <!-->&gt;<!--> <span>0.5) while Vismed Multi did not adversely affect the corneal integrity. Glucose/lactate concentrations remained unchanged while lubricants<span> were applied. Histology revealed epithelial loss and severe alterations of the superficial stroma for Optive. Optive Fusion displayed a comparable pathology. Neopt did not significantly affect the corneal healing and integrity.</span></span></p><p>This study suggested a cumulative corneal toxicity of Optive and, to a lesser extent, Optive Fusion most likely caused by its oxidative preservative, SOC. Clinical data are needed to clarify the application frequency at which corneal toxicity might occur. Neopt and Vismed Multi did not affect the corneal integrity.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 3","pages":"Pages 123-129"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2016.12.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71813906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"editorial board (IFC)","authors":"","doi":"10.1016/S0940-2993(17)30064-7","DOIUrl":"https://doi.org/10.1016/S0940-2993(17)30064-7","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 3","pages":"Page IFC"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0940-2993(17)30064-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71814434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Zika virus infection induces spinal cord injury and encephalitis in newborn Swiss mice.","authors":"N. Fernandes, J. Nogueira, R. Réssio, C. Cirqueira, L. Kimura, Karolina R Fernandes, M. S. Cunha, R. Souza, J. Guerra","doi":"10.1016/j.etp.2016.11.004","DOIUrl":"https://doi.org/10.1016/j.etp.2016.11.004","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"15 1","pages":"63-71"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76987810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}