Experimental and Toxicologic Pathology最新文献

{"title":"Reference control data obtained from an in vivo comet-micronucleus combination assay using Sprague Dawley rats","authors":"Sawako Kasamoto ,&nbsp;Shoji Masumori ,&nbsp;Jin Tanaka ,&nbsp;Maya Ueda ,&nbsp;Masahito Fukumuro ,&nbsp;Miho Nagai ,&nbsp;Jyoji Yamate ,&nbsp;Makoto Hayashi","doi":"10.1016/j.etp.2017.01.002","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.002","url":null,"abstract":"<div><p><span>According to the International Conference on Harmonization Guidance on Genotoxicity Testing and Data Interpretation for Pharmaceuticals Intended for Human Use (ICH S2(R1)), a positive response in any </span><em>in vitro</em> assay necessitates additional <em>in vivo</em><span> test(s) (other tissue/endpoint) in addition to the erythrocyte micronucleus<span> test when Option 1 of the test battery is selected. When Option 2 of the test battery is selected, a bacterial gene mutation test and two </span></span><em>in vivo</em> tests with different tissues/endpoint are required. The <em>in vivo</em><span> alkaline comet assay is recommended as the second </span><em>in vivo</em><span> test because it can detect a broad spectrum of DNA damage in any tissue and can be combined with the erythrocyte micronucleus test. Considering animal welfare, a combination assay is preferable to an individual assay. Thus, we validated the protocol for the </span><em>in vivo</em> comet-micronucleus combination assay in rats with three daily administrations and determined the dose of the positive control (ethyl methanesulfonate; EMS, 200<!--> <!-->mg/kg/day). We also collected the negative control (vehicle) and positive control (EMS) data from the comet (liver, stomach, and kidney) and micronucleus (bone marrow) combination assay using male Sprague Dawley (SD) rats. The negative control data were comparable to our historical control data obtained from stand-alone assays. The positive control data showed clear and consistent positive responses in both endpoints.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 4","pages":"Pages 187-191"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.01.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71811993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory effects of the selective phosphodiesterase 3 inhibitor, cilostazol, and antioxidants, enzymatically-modified isoquercitrin and α-lipoic acid, reduce dextran sulphate sodium-induced colorectal mucosal injury in mice.","authors":"Yumi Kangawa, Toshinori Yoshida, Hajime Abe, Yoshiki Seto, Taishi Miyashita, Michi Nakamura, T. Kihara, S. Hayashi, M. Shibutani","doi":"10.1016/j.etp.2016.12.004","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.004","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"10 1","pages":"179-186"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82020320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatotoxicity induced by radix Sophorae tonkinensis in mice and increased serum cholinesterase as a potential supplemental biomarker for liver injury.","authors":"Liping Wang, Jinyao Lu, Wei Sun, Y. Gu, Chaochao Zhang, R. Jin, Lingyong Li, Zean Zhang, X. Tian","doi":"10.1016/j.etp.2017.01.003","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.003","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"2 1","pages":"193-202"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87098110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reference control data obtained from an in vivo comet-micronucleus combination assay using Sprague Dawley rats.","authors":"S. Kasamoto, S. Masumori, J. Tanaka, Maya Ueda, M. Fukumuro, M. Nagai, J. Yamate, M. Hayashi","doi":"10.1016/j.etp.2017.01.002","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.002","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"140 1","pages":"187-191"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72929080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold nanoparticles ameliorate acetaminophen induced hepato-renal injury in rats","authors":"Mohd Salim Reshi,&nbsp;Sadhana Shrivastava,&nbsp;Amita Jaswal,&nbsp;Neelu Sinha,&nbsp;Chhavi Uthra,&nbsp;Sangeeta Shukla","doi":"10.1016/j.etp.2017.01.009","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.009","url":null,"abstract":"<div><p><span><span>Valuable effects of gold particles have been reported and used in complementary medicine<span><span> for decades. The aim of this study was to evaluate the therapeutic efficacy of gold nanoparticles (AuNPs) against </span>acetaminophen (APAP) induced toxicity. </span></span>Albino rats were administered APAP at a dose of 2</span> <!-->g/kg <em>p.o.</em> once only. After 24<!--> <!-->h of APAP intoxication, animals were treated with three different doses of AuNPs (50<!--> <!-->μg/kg, 100<!--> <!-->μg/kg, 150<!--> <span>μg/kg) orally or silymarin at a dose of 50</span> <!-->mg/kg <em>p.o.,</em> once only. Animals of all the groups were sacrificed after 24<!--> <span><span><span>h of last treatment<span>. APAP administered group showed a significant rise in the AST, ALT, SALP, LDH, cholesterol, </span></span>bilirubin<span><span><span>, albumin, urea and creatinine in serum which indicated the hepato-renal damage. A significantly enhanced LPO<span><span> and a depleted level of GSH were observed in APAP intoxicated rats. Declined activities of SOD and </span>Catalase, after acetaminophen exposure indicated </span></span>oxidative stress in liver and </span>kidney<span><span>. The activities of ATPase and glucose-6-Phosphatase were significantly inhibited after APAP administration. AuNPs treatment reversed all variables significantly towards normal level and was found nontoxic. Thus it is concluded that gold nanoparticles played a beneficial role in reducing acetaminophen induced toxicity and can be used in the development of </span>drug against hepatic as well as </span></span></span>renal diseases, after further preclinical and clinical studies.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 4","pages":"Pages 231-240"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.01.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71811989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The ability of hesperidin compared to that of insulin for preventing osteoporosis induced by type I diabetes in young male albino rats: A histological and biochemical study.","authors":"A. S. Shehata, Mona G. Amer, M. R. Abd El-Haleem, R. Karam","doi":"10.1016/j.etp.2017.01.008","DOIUrl":"https://doi.org/10.1016/j.etp.2017.01.008","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"3 1","pages":"203-212"},"PeriodicalIF":0.0,"publicationDate":"2017-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88945785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extract of bulbus Fritillaria cirrhosa perturbs spindle assembly checkpoint, induces mitotic aberrations and genomic instability in human colon epithelial cell line","authors":"Xihan Guo ,&nbsp;Juan Ni ,&nbsp;Jinglun Xue ,&nbsp;Xu Wang","doi":"10.1016/j.etp.2016.12.009","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.009","url":null,"abstract":"<div><h3>Background</h3><p>Bulbus <span><em>Fritillaria</em><em> cirrhosa</em></span><span><span><span><span> D. Don (BFC) has been used in China as a folk medicine for the </span>treatment of cough and asthma for more than 2000 years. The </span>antitussive and </span>antiasthmatic<span> effects of BFC have been reported before, nevertheless its toxicity and safety have not been documented. This study investigated the possible effects of BFC on spindle assembly checkpoint<span> (SAC), mitotic fidelity and genomic stability in human NCM460 colon epithelial cells.</span></span></span></p></div><div><h3>Methods</h3><p>Cells were treated with BFC (0, 20, 40, 80 and 160<!--> <!-->μg/ml) for 24, 48 and 72<!--> <span><span>h and harvested differently according to the biomarkers observed. Mitotic aberrations were assessed by the biomarkers of chromosome misalignment (CMA), chromosome lagging (CL) and chromatin bridge (CB). Frequencies of </span>micronuclei<span> (MN), nucleoplasmic bridge and nuclear bud (NB) in cytokinesis-block micronucleus assay were used as indicators of genomic instability (GIN). SAC activity was determined by anaphase to metaphase ratio (AMR) and the expression of several SAC genes, including </span></span><em>CENP-E, Mps1</em>, <em>Bub1</em>, <em>Mad-1</em>, <em>BubR1</em> and <em>Mad-2</em>.</p></div><div><h3>Results</h3><p>Compared with the control, cells in BFC treated groups (80 and 160<!--> <!-->μg/ml) showed: 1) increased AMR (p<!--> <!-->&lt;<!--> <!-->0.05), up-regulated expression of <em>Mps1</em>, <em>Bub1</em> and <em>Mad-1</em> (p<!--> <!-->&lt;<!--> <!-->0.05) and down-regulated expression of <em>CENP-E, BubR1</em> and <em>Mad-2</em> (p<!--> <!-->&lt;<!--> <!-->0.05); 2) increased frequencies of CMA, CL and CB (p<!--> <!-->&lt;<!--> <!-->0.01); 3) increased incidences of MN and NB (p<!--> <!-->&lt;<!--> <!-->0.01).</p></div><div><h3>Conclusions</h3><p>This study revealed for the first time that BFC causes mitotic aberrations and GIN in human colon epithelial cells and these effects maybe the result of SAC dysfunction.</p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 3","pages":"Pages 163-171"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2016.12.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71814419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of tannic acid on the bone tissue of adult male Wistar rats exposed to cadmium and lead","authors":"Ewa Tomaszewska ,&nbsp;Piotr Dobrowolski ,&nbsp;Anna Winiarska-Mieczan ,&nbsp;Małgorzata Kwiecień ,&nbsp;Agnieszka Tomczyk ,&nbsp;Siemowit Muszyński","doi":"10.1016/j.etp.2016.12.003","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.003","url":null,"abstract":"<div><p><span>Toxic elements such as cadmium (Cd) and lead (Pb) accumulate to the largest extent in bones. Rats at the age of 12 weeks were used to check whether tannic acid (TA) at the concentration of 0.5%, 1.0%, 1.5%. 2.0% or 2.5% would have a protective effect on the structure and properties of bones in the case of exposure to Cd and Pb (diet: 7</span> <!-->mg Cd/kg and 50<!--> <span><span><span>mg Pb/kg) for 12 weeks. The effects of administration of TA in Cd- and Pb-poisoned rats on bone mechanical and geometric properties, trabecular histomorphometry<span> as well as the morphology of articular and growth cartilages were determined. All the rats co-exposured to Cd and Pb had enhanced heavy metals<span> concentration in blood plasma and bone and reduced bone Ca content irrespective of the tannic acid administration. Heave metals given to adult rats did not influence the morphology and geometry of the femur, but reduced the mechanical endurance and histomorphometric parameters of trabecular bone<span> irrespective of the treatment. A diet rich in TA improved </span></span></span></span>articular cartilage and </span>growth plate constituents in heavy metal-poisoned rats, as indicated by the measurement of the thickness of particular zones. It seems that a use of alimentary TA supplementation in adult rats can counteract, in a dose-dependent manner, only some of the destructive changes evoked by Cd and Pb excess.</span></p></div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 3","pages":"Pages 131-141"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2016.12.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71813907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of tannic acid on the bone tissue of adult male Wistar rats exposed to cadmium and lead.","authors":"E. Tomaszewska, P. Dobrowolski, A. Winiarska-Mieczan, M. Kwiecień, A. Tomczyk, S. Muszyński","doi":"10.1016/j.etp.2016.12.003","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.003","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"53 1","pages":"131-141"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78830109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of Tritone (Livosone) on oxidative DNA damage and its hepatoprotective potential against various hepatotoxic agent in wistar rats.","authors":"S. K. Medhekar, T. P. Jadhav, Vishal Sadashiv Sasane, V. Shende, N. Aloorkar, Anjali Baburao Chincholkar, G. Soman, A. Kulkarni","doi":"10.1016/j.etp.2016.12.005","DOIUrl":"https://doi.org/10.1016/j.etp.2016.12.005","url":null,"abstract":"","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"15 1","pages":"153-161"},"PeriodicalIF":0.0,"publicationDate":"2017-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87984273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}