Indian Journal of Dermatology Venereology & Leprology最新文献

{"title":"Autoantibody detection by conventional and novel mosaic BIOCHIP technique: A comparative analysis for the diagnosis of autoimmune bullous diseases.","authors":"Punya Suvarna, Raghavendra Rao","doi":"10.25259/IJDVL_1089_2024","DOIUrl":"10.25259/IJDVL_1089_2024","url":null,"abstract":"<p><p>Background Circulating autoantibodies in patients with autoimmune bullous diseases can be detected by indirect immunofluorescence (IIF) microscopy. The sensitivity of this method depends on the substrate used. Normal human skin (NHS) and salt-split skin (SSS) are widely used in conventional serodiagnosis of autoimmune bullous diseases. A novel mosaic biochip has been reported to be a highly sensitive and specific test to detect the circulating antibodies in this subset of patients. Objectives This study was designed to compare IIF microscopy by conventional and mosaic biochip technologies in the serodiagnosis of patients with autoimmune bullous diseases. Methods This cross-sectional study included sera of 103 patients with autoimmune bullous diseases. Conventional IIF microscopy was carried out using NHS and SSS substrates. IIF using the mosaic biochip was performed as per the manufacturer's instructions. Results The conventional technique detected intercellular staining with IgG in 62 patients, and the mosaic biochip detected it in 56 monkey oesophageal samples. The latter also detected both desmoglein (Dsg) 1 and 3 in 45 patients and Dsg 1 and 3 individually in eight and ten serum samples, respectively. Both techniques detected epidermal staining in the SSS of 37 patients with sub-epidermal autoimmune bullous diseases, and dermal staining was observed in seven sera. Seventeen patients with epidermal staining patterns revealed antibodies to BP180 only; six patients showed reactivity to both BP 180 and 230. Limitation Due to financial constraints, sera of healthy controls could not be studied. Conclusions The BIOCHIP mosaic IIF is an useful adjunct IIF technique. It not only helps to detect the staining pattern but also identify the target antigens in common autoimmune bullous disease. Sensitivity of conventional IIF in detecting ICS is 93.9% in contrast to 84.4% with the BIOCHIP mosaic technique. However, mosaic BIOCHIP technique helped to identify the target antigens in common AIBD.</p>","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":" ","pages":"482-487"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dermoscopic cues of Paget disease: A series of three cases.","authors":"Bhavni Oberoi, Durga Madhab Tripathy, Shekhar Neema, Eeshaan Ranjan, Satish Chand, Shobhit Goel","doi":"10.25259/IJDVL_199_2024","DOIUrl":"10.25259/IJDVL_199_2024","url":null,"abstract":"","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":" ","pages":"534-536"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin coloured asymptomatic facial plaque in a woman.","authors":"Ruby Jain, Subhra Dhar","doi":"10.25259/IJDVL_1088_2023","DOIUrl":"10.25259/IJDVL_1088_2023","url":null,"abstract":"","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":" ","pages":"550-552"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baricitinib in the management of persistent actinic granuloma: An innovative therapeutic approach.","authors":"Ran An, Junting Tang, Hao-Xue Lei, Yin Tu","doi":"10.25259/IJDVL_554_2024","DOIUrl":"10.25259/IJDVL_554_2024","url":null,"abstract":"","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":" ","pages":"521-523"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic biomarkers and altered phenylalanine metabolic pathway in preschool children with atopic dermatitis - A pilot study.","authors":"Jia Yu, Ting Chen, He Zhou, Sujun Li, Bo Wu, Ying Xiong","doi":"10.25259/IJDVL_1125_2023","DOIUrl":"10.25259/IJDVL_1125_2023","url":null,"abstract":"<p><p>Background Atopic dermatitis (AD) has high prevalence in children. Current AD diagnosis and management focuses only on clinical phenotypes, but do not explore the endophenotypes, which are more important because they are a series of biomarkers linking clinical phenotype and genotype Aims Metabolomics can qualitatively and quantitatively capture real-time dynamic changes in a wide range of small molecule metabolites. This pilot study evaluated metabolomics biomarkers and altered metabolic pathways in preschool children with AD, aiming to explore the underlying molecular mechanisms and signalling pathways of the disease. Methods Blood samples of 23 preschool children with AD and 23 healthy children without AD or any other skin disease were collected. The untargeted metabolomic measurements were performed on a SCIEX-AD ultraperformance liquid chromatography system coupled with an AB SCIEX X500B QTOF system. Characteristics of small molecules in AD children were assessed and their associations with AD clinical index were evaluated. Altered metabolic pathways in AD children were also analysed using a comprehensive metabolomics platform. Results A total of 1,969 metabolites were identified, of which AD children exhibited 377 significantly altered metabolites. Multivariate statistical analysis demonstrated that the AD group and the control group could be clearly separated. Volcano plot analysis illustrated that 144 metabolites were up-regulated and 233 metabolites were down-regulated in AD children. The Severity Scoring of Atopic Dermatitis (SCORAD index) showed a moderate-to-strong association with estrogens, carotenes, leukotrienes, flavonols and keto acids in AD children (|r|=0.440-0.557). Several pathways, including the phenylalanine metabolism, were identified as altered in AD children. Limitations A small group of children was included in the study; the results need to be validated in larger sample sizes. Conclusion Results of this study illustrate potential alterations in metabolites and the phenylalanine metabolic pathway in preschool children with AD. Although this is a pilot study with a limited sample size, it may provide a new perspective for exploring the pathogenesis of AD, and for personalised treatment modalities.</p>","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":" ","pages":"440-447"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary drug resistant cutaneous tuberculosis: A retrospective case series of seven patients.","authors":"","doi":"10.25259/IJDVL_63_2023_ER","DOIUrl":"https://doi.org/10.25259/IJDVL_63_2023_ER","url":null,"abstract":"","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":"91 4","pages":"567"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144530875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intradermal injection of botulinum toxin for erythema in rosacea: A scoping review and meta-analysis.","authors":"Marvin Chia-Han Yeh, Ya-Chu Shih, Yu-Chen Huang","doi":"10.25259/IJDVL_274_2024","DOIUrl":"10.25259/IJDVL_274_2024","url":null,"abstract":"<p><p>Background Rosacea is a skin condition characterised by persistent facial erythema, flushing, papules, pustules, and telangiectasia. Botulinum toxin A (BoNT-A) has been used to treat a variety of conditions, but its effectiveness in improving facial erythema in rosacea patients is uncertain. Objectives The aim of the study is to evaluate the effectiveness and determine the optimal dose of BoNT-A treatment for rosacea. Methods An online database search (Pubmed, Cochrane Library and Embase) was conducted on 30th June 2023 to identify studies that used intradermal injection of BoNT-A to treat facial erythema in rosacea patients and excluded studies in which BoNT-A was used for facial erythema due to other known medical condition such as menopause, drug or pregnancy. The primary outcome measure for this study was the improvement in erythema score as objectively assessed. A random effect model was used in the meta-analysis. Results Seven studies involving a total of 167 rosacea patients were included in the meta-analysis. Meta-analysis of two randomised controlled trials showed improvement of erythema on the third month after treatment standardized mean difference (SMD): 1.676, 95% confidence interval (CI): 2.278-1.074, I2: 35.76%). A separate analysis of seven single-armed treatment studies found significant improvement in erythema with intradermal injection of BoNT-A at one, two and three months after treatment (first month: SMD: 2.712, 95% CI: 4.1182-1.243; second month: SMD:2.213, 95% CI: 3.702-0.725; third month: SMD: 1.912, 95% CI: 2.882-0.941). Adverse events, including mild facial paralysis and injectional purpura, were reported in some studies. Limitation The limitations of this study include heterogeneity in study design and a small sample size. Conclusion Intradermal injection of BoNT-A may be an effective treatment for facial erythema in rosacea. Unwanted facial muscle paralysis was seen in different BoNT-A concentration but not noted when the dose was less than 0.02ml per site. Future studies particularly randomised trials are required to identify the volume of injection required to reduce the erythema.</p>","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":" ","pages":"448-454"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ambispective cross-sectional descriptive study to characterise the cutaneous and histological features of pediatric Langerhans cell histiocytosis (LCH).","authors":"Avik Mondal, Vishal Gaurav, Sujay Khandpur, Aditya Kumar Gupta, Shipra Agarwal, Rachna Seth, M Ramam, Neetu Bhari","doi":"10.25259/IJDVL_1217_2024","DOIUrl":"10.25259/IJDVL_1217_2024","url":null,"abstract":"<p><p>Background Langerhans cell histiocytosis (LCH) is an idiopathic disorder characterised by the presence of pathological Langerhans cells derived from myeloid precursor cells. There is a scarcity of data regarding its diverse cutaneous and histological presentations, as well as their prognostic implications. Objectives To investigate the clinical and histological features in children with LCH and explore the correlation between the frequency and severity of systemic involvement and various clinical and histopathological findings. Methods A two-year ambispective cross-sectional descriptive study was conducted at a tertiary referral centre to identify and describe the clinical and histological features of LCH. Twenty patients (10 prospective and 10 retrospective) were diagnosed based on histopathology. Results This study included 20 patients with LCH, majority in the age range of 1-5 years and with male predominance (75%). The primary complaint was fever (100%), accompanied by itching (65%), abdominal pain (45%), or respiratory issues (40%). Scalp involvement was universal, with erythematous papules (95%) being the most common type of lesions. Systemic examination revealed hepatomegaly (65%), splenomegaly (40%), and cervical lymphadenopathy (50%). Histopathological evaluation from varied skin lesions revealed dense dermal infiltrates of Langerhans cells, with papillary dermal oedema and significant RBC extravasation. Immunohistochemistry confirmed Langerhans cells. Systemic evaluation indicated organ involvement in varying percentages. Limitations Small sample size and retrospective nature of half of the cases are major limitations of our study. Conclusion This study shows skin lesions may develop as crops and fever is a consistent feature found on history or at presentation which may act as important clues towards clinical diagnosis of LCH. In terms of histological features, papillary dermal oedema and RBC extravasation are some prominent findings. Histopathological features does not predict the progression of disease and the extent of systemic involvement.</p>","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":" ","pages":"488-495"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosacea: Considerations for a better understanding of the disease.","authors":"William Wd James","doi":"10.25259/IJDVL_1789_2024","DOIUrl":"10.25259/IJDVL_1789_2024","url":null,"abstract":"","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":" ","pages":"564-566"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of polysensitivity in allergic contact dermatitis: A five-year retrospective study.","authors":"Channaraya Aradita, Srihari Sahana, P S S Ranugha, Garehatty Rudrappa Kanthraj","doi":"10.25259/IJDVL_880_2024","DOIUrl":"10.25259/IJDVL_880_2024","url":null,"abstract":"","PeriodicalId":50376,"journal":{"name":"Indian Journal of Dermatology Venereology & Leprology","volume":" ","pages":"544-546"},"PeriodicalIF":3.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}