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Construction and development of new rabies virus vectors carrying enhanced green fluorescent protein 构建和开发携带增强型绿色荧光蛋白的新型狂犬病毒载体
Future Virology Pub Date : 2024-01-25 DOI: 10.2217/fvl-2023-0168
Seyed Hamidreza Naghi Mousavi, Alireza Gholami, Mojtaba Jafarinia, Mehdi Ajorloo
{"title":"Construction and development of new rabies virus vectors carrying enhanced green fluorescent protein","authors":"Seyed Hamidreza Naghi Mousavi, Alireza Gholami, Mojtaba Jafarinia, Mehdi Ajorloo","doi":"10.2217/fvl-2023-0168","DOIUrl":"https://doi.org/10.2217/fvl-2023-0168","url":null,"abstract":"Aim: Rabies is one of the most lethal viral diseases. This study developed a recombinant rabies virus, PVEGFP, expressing an EGFP protein based on PV strain to be applied in virus-neutralizing antibody (VNA) titration methods. Methods: The EGFP gene was inserted into the PVMCS strain, a previously discussed recombinant virus. The recombinant virus was applied in the rapid fluorescent focus inhibition test (RFFIT) VNA titration method, and six vaccinated persons were studied for validation. Results: The recombinant rabies strain showed similar propagation properties to its ancestry strain, PVMCS. It could also measure VNA titers in the RFFIT method not significantly different from those acquired using the CVS-11 virus. Conclusion: Utilizing the PVEGFP strain in the RFFIT method can produce rapid, reliable and affordable results.","PeriodicalId":503758,"journal":{"name":"Future Virology","volume":"18 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139596062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative and phylogenetic analysis of Epstein–Barr virus isolates in gastric cancer and chronic gastritis patients in Iran 伊朗胃癌和慢性胃炎患者中 Epstein-Barr 病毒分离株的比较和系统发育分析
Future Virology Pub Date : 2024-01-10 DOI: 10.2217/fvl-2023-0144
Behrang Sarshari, Raziyeh Zareh-Khoshchehreh, Mehdi Tavallaei, Mohsen Rajabnia, Mohammad Tayefeh Norooz, S. Mohebbi, M. Ravanshad
{"title":"Comparative and phylogenetic analysis of Epstein–Barr virus isolates in gastric cancer and chronic gastritis patients in Iran","authors":"Behrang Sarshari, Raziyeh Zareh-Khoshchehreh, Mehdi Tavallaei, Mohsen Rajabnia, Mohammad Tayefeh Norooz, S. Mohebbi, M. Ravanshad","doi":"10.2217/fvl-2023-0144","DOIUrl":"https://doi.org/10.2217/fvl-2023-0144","url":null,"abstract":"Aim: To investigate the correlation between Epstein–Barr virus (EBV) variants and EBV-associated gastric cancer (EBVaGC) in Iran. Materials & methods: Biopsies from 21 gastric cancer (4 positive EBER-ISH (EBVaGC) and 17 negative EBER-ISH) and 11 gastritis patients were analyzed using nested PCR and sequencing. Results: Genotype 1 (75%) was the predominant EBV genotype. The P-thr variant was the most common in all groups (positive EBER-ISH 75%, negative EBER-ISH 76.4%, and gastritis 81.8%), followed by P-ala. Additionally, the predominant LMP1 strain in all groups was Med. Sequencing analysis revealed a strong correlation between the P-thr and type 1. Conclusion: Since no preferential correlations were observed between distinct EBV variants and the study groups, these results might indicate geographic polymorphisms.","PeriodicalId":503758,"journal":{"name":"Future Virology","volume":"88 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139440488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of the COVID-19 pandemic on the reporting of Crimean–Congo hemorrhagic fever COVID-19 大流行对克里米亚-刚果出血热报告的影响
Future Virology Pub Date : 2023-11-15 DOI: 10.2217/fvl-2023-0129
M. Pouriayevali, Ehsan Mostafavi, M. Fazlalipour, T. Jalali, S. Khakifirouz, Seyed Dawood Mosavi-Nasab, Mostafa Salehi-Vaziri
{"title":"Effect of the COVID-19 pandemic on the reporting of Crimean–Congo hemorrhagic fever","authors":"M. Pouriayevali, Ehsan Mostafavi, M. Fazlalipour, T. Jalali, S. Khakifirouz, Seyed Dawood Mosavi-Nasab, Mostafa Salehi-Vaziri","doi":"10.2217/fvl-2023-0129","DOIUrl":"https://doi.org/10.2217/fvl-2023-0129","url":null,"abstract":"","PeriodicalId":503758,"journal":{"name":"Future Virology","volume":"105 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inactivated Sendai Virus Induces Reactive Oxygen species-mediated Mitochondrial Apoptosis in Human Renal Carcinoma Cells 灭活仙台病毒诱导活性氧介导的人肾癌细胞线粒体凋亡
Future Virology Pub Date : 2020-03-01 DOI: 10.2217/fvl-2019-0102
Lei Zhou, Shuya Sun, Qing Li, Liying Shi
{"title":"Inactivated Sendai Virus Induces Reactive Oxygen species-mediated Mitochondrial Apoptosis in Human Renal Carcinoma Cells","authors":"Lei Zhou, Shuya Sun, Qing Li, Liying Shi","doi":"10.2217/fvl-2019-0102","DOIUrl":"https://doi.org/10.2217/fvl-2019-0102","url":null,"abstract":"","PeriodicalId":503758,"journal":{"name":"Future Virology","volume":"7 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141227921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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