International Journal of Developmental Biology最新文献

{"title":"Insights into the mechanism of adult neurogenesis - an interview with Arturo Álvarez-Buylla.","authors":"Diana Escalante-Alcalde,&nbsp;Jesús Chimal-Monroy","doi":"10.1387/ijdb.200297de","DOIUrl":"https://doi.org/10.1387/ijdb.200297de","url":null,"abstract":"<p><p>Neurogenesis is the process by which new neurons are formed from progenitor cells. The adult nervous system was long considered unable to generate new neurons, especially in mammals. It was not until the 1960s that Joseph Altman and Gopal Das, using H³-thymidine autoradiography to trace newly formed cells, that the first suggestions of new neurons added to the olfactory bulb and the dentate gyrus of the rat hippocampus came about. These observations remained controversial for many years as they went against the dogmatic view that the structure of the adult brain precluded processes of neurogenesis. It was not until two decades later that work in songbirds and then in mammals, not only confirmed that new neurons could be produced in the adult brain, but revealed basic processes of how young neurons are produced, how they could migrate long distances and become incorporated into adult brain circuits. Arturo Álvarez-Buylla has made important contributions to the understanding of the mechanism of adult neurogenesis, including the identification of adult neural stem cells. Here we summarize a discussion with him related to the field of adult neurogenesis, the root of his interest in neural development and the ramifications of some of his laboratory findings.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.200297de","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38610193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial distribution of blood vessels in the chick embryo chorioallantoic membrane.","authors":"Diego Guidolin,&nbsp;Roberto Tamma,&nbsp;Tiziana Annese,&nbsp;Cinzia Tortorella,&nbsp;Domenico Ribatti","doi":"10.1387/ijdb.210151dr","DOIUrl":"https://doi.org/10.1387/ijdb.210151dr","url":null,"abstract":"<p><p>The chick embryo chorioallantoic membrane (CAM) is a useful tool with which to study both angiogenesis and anti-angiogenesis in vivo. CAM vascular growth pattern - including the way through vessels fills the available space - can be quite easily described and quantified using image analysis procedures, in order to evaluate different parameters, including fractal dimension, lacunarity and non-fractal order-disorder parameters. In the present study, we further expanded this morphological description, by estimating an index expressing the degree of symmetry characterizing the CAM vascular tree structure in the course of the embryonic development. Moreover, a uniformity index was estimated quantitatively to characterize the space-filling features of the vessels, i.e. the degree of spatial uniformity of their distribution in the tissue.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39705316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conserved roles of <i>Rax/rx3</i> genes in hypothalamus and pituitary development.","authors":"Flávio S J De Souza,&nbsp;Marysia Placzek","doi":"10.1387/ijdb.200081fd","DOIUrl":"https://doi.org/10.1387/ijdb.200081fd","url":null,"abstract":"<p><p><i>Rax</i> (<i>Rx</i>) genes encode paired-type homeodomain-containing transcription factors present in virtually all metazoan groups. In vertebrates, studies in fish, amphibian, chick and mouse models have revealed that these genes play important roles in the development of structures located at the anterior portion of the central nervous system, in particular the eyes, the hypothalamus and the pituitary gland. In addition, human patients with eye and brain defects carry mutations in the two human <i>Rax</i> paralogues, <i>RAX</i> and <i>RAX2</i>. Here, we review work done in the last years on <i>Rax</i> genes, focusing especially on the function that mouse <i>Rax</i> and its zebrafish homologue, <i>rx3</i>, play in hypothalamic and pituitary development. Work on both of these model organisms indicate that <i>Rax</i> genes are necessary for the patterning, growth and differentiation of the hypothalamus, in particular the ventro-tuberal and dorso-anterior hypothalamus, where they effect their action by controlling expression of the secreted signalling protein, Sonic hedgehog (Shh). In addition, <i>Rax/rx3</i> mutations disturb the development of the pituitary gland, mimicking phenotypes observed in human subjects carrying mutations in the <i>RAX</i> gene. Thus, along with their crucial role in eye morphogenesis, <i>Rax</i> genes play a conserved role in the development of the hypothalamus and adjacent structures in the vertebrate clade.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.200081fd","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38382572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the maize Mediator CDK8 module and transposon-mediated mutagenesis of <i>ZmMed12a</i>.","authors":"Ana L Alonso-Nieves,&nbsp;Tania Núñez-Ríos,&nbsp;Julio A Massange-Sánchez,&nbsp;Kevin R Ahern,&nbsp;Daniel Lepe-Soltero,&nbsp;Marcelina García-Aguilar,&nbsp;Thomas P Brutnell,&nbsp;Ruairidh J H Sawers,&nbsp;C Stewart Gillmor","doi":"10.1387/ijdb.200098sg","DOIUrl":"https://doi.org/10.1387/ijdb.200098sg","url":null,"abstract":"<p><p>Mediator is a conserved transcriptional co-activator that links transcription factors bound at enhancer elements to RNA Polymerase II. Mediator-RNA Polymerase II interactions can be sterically hindered by the Cyclin Dependent Kinase 8 (CDK8) module, a submodule of Mediator that acts to repress transcription in response to discrete cellular and environmental cues. The CDK8 module is conserved in all eukaryotes and consists of 4 proteins: CDK8, CYCLIN C (CYCC), MED12, and MED13. In this study, we have characterized the CDK8 module of Mediator in maize using genomic, molecular and functional resources. The maize genome contains single copy genes for <i>Cdk8</i>, <i>CycC</i>, and <i>Med13</i>, and two genes for <i>Med12</i>. Analysis of expression data for the CDK8 module demonstrated that all five genes are broadly expressed in maize tissues, and change their expression in response to phosphate and nitrogen limitation. We performed <i>Dissociation (Ds)</i> insertional mutagenesis, recovering two independent insertions in the <i>ZmMed12a</i> gene, one of which produces a truncated transcript. Our molecular identification of the maize CDK8 module, assays of CDK8 module expression under nutrient limitation, and characterization of transposon insertions in <i>ZmMed12a</i> establish the basis for molecular and functional studies of the role of these important transcriptional regulators in development and nutrient homeostasis in <i>Zea mays</i>.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38382574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitotic timing is differentially controlled by A- and B-type cyclins and by CDC6 associated with a <i>bona fide</i> CDK inhibitor Xic1 in <i>Xenopus laevis</i> cell-free extract.","authors":"Mohammed El Dika,&nbsp;Lisa Wechselberger,&nbsp;Bilal Djeghout,&nbsp;Djamel Eddine Benouareth,&nbsp;Krystyna Jęderka,&nbsp;Sławomir Lewicki,&nbsp;Robert Zdanowski,&nbsp;Claude Prigent,&nbsp;Malgorzata Kloc,&nbsp;Jacek Z Kubiak","doi":"10.1387/ijdb.200313jk","DOIUrl":"https://doi.org/10.1387/ijdb.200313jk","url":null,"abstract":"<p><p>The timing of the M-phase is precisely controlled by a CDC6-dependent mechanism inhibiting the mitotic histone H1 kinase. Here, we describe the differential regulation of the dynamics of this mitotic kinase activity by exogenous cyclin A or cyclin B in the <i>Xenopus laevis</i> cycling extracts. We show that the experimental increase in cyclin A modifies only the level of histone H1 kinase activity, while the cyclin B increase modifies two parameters: histone H1 kinase activity and the timing of its full activation, which is accelerated. On the other hand, the cyclin A depletion significantly delays full activation of histone H1 kinase. However, when CDC6 is added to such an extract, it inhibits cyclin B-associated histone H1 kinase, but does not modify the mitotic timing in the absence of cyclin A. Further, we show via p9 co-precipitation with Cyclin-Dependent Kinases (CDKs), that both CDC6 and the <i>bona fide</i> CDK1 inhibitor Xic1 associate with the mitotic CDKs. Finally, we show that the Xic1 temporarily separates from the mitotic CDKs complexes during the peak of histone H1 kinase activity. These data show the differential coordination of the M-phase progression by cyclin A- and cyclin B-dependent CDKs, confirm the critical role of the CDC6-dependent histone H1 kinase inhibition in this process, and show that CDC6 acts differentially through the cyclin B- and cyclin A-associated CDKs. This CDC6- and cyclins-dependent mechanism likely depends on the precisely regulated association of Xic1 with the mitotic CDKs complexes. We postulate that: i. the dissociation of Xic1 from the CDKs complexes allows the maximal activation of CDK1 during the M-phase, ii. the switch between cyclin A- and cyclin B-CDK inhibition upon M-phase initiation may be responsible for the diauxic growth of mitotic histone H1 kinase activity.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39437353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The subterranean catfish <i>Phreatobius cisternarum</i> provides insights into visual adaptations to the phreatic environment.","authors":"Louise N Perez,&nbsp;Bertha R Mariluz,&nbsp;Jamily Lorena,&nbsp;Amy Liu,&nbsp;Marcos P Sousa,&nbsp;Rodrigo A P Martins,&nbsp;John S Taylor,&nbsp;Patricia N Schneider","doi":"10.1387/ijdb.200335pn","DOIUrl":"https://doi.org/10.1387/ijdb.200335pn","url":null,"abstract":"<p><p>Vertebrate eyes share the same general organization, though species have evolved morphological and functional adaptations to diverse environments. Cave-adapted animals are characterized by a variety of features including eye reduction, loss of body pigmentation, and enhanced non-visual sensory systems. Species that live in perpetual darkness have also evolved sensory mechanisms that are independent of light stimuli. The subterranean catfish <i>Phreatobius cisternarum</i> lives in the Amazonian phreatic zone and displays a diversity of morphological features that are similar to those observed in cavefish and appear to be adaptations to life in the dark. Here we combine histological and transcriptome analyses to characterize sensory adaptations of <i>P. cisternarum</i> to the subterranean environment. Histological analysis showed that the vestigial eyes of <i>P. cisternarum</i> contain a rudimentary lens. Transcriptome analysis revealed a repertoire of eleven visual and non-visual opsins and the expression of 36 genes involved in lens development and maintenance. In contrast to other cavefish species, such as <i>Astyanax mexicanus</i>, <i>Phreatichthys andruzzii, Sinocyclocheilus anophthalmus</i> and <i>Sinocyclocheilus microphthalmus</i>, DASPEI neuromast staining patterns did not show an increase in the number of sensory hair cells. Our work reveals unique adaptations in the visual system of <i>P. cisternarum</i> to underground habitats and helps to shed light into troglomorphic attributes of subterranean animals.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38758839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virtual meeting, real and sound science: report of the 17^th Meeting of the Spanish Society for Developmental Biology (SEBD-2020).","authors":"Sofia J Araújo,&nbsp;Isabel Almudi,&nbsp;Laura Bozal-Basterra,&nbsp;Fernando Casares,&nbsp;Sergio Casas-Tintó,&nbsp;Augusto Escalante,&nbsp;Fernando García-Moreno,&nbsp;María Losada-Pérez,&nbsp;Ignacio Maeso,&nbsp;Luciano Marcon,&nbsp;Oscar Ocaña,&nbsp;Olatz Pampliega,&nbsp;Álvaro Rada-Iglesias,&nbsp;Teresa Rayon,&nbsp;James Sharpe,&nbsp;James D Sutherland,&nbsp;Cristina Villa Del Campo,&nbsp;Rosa Barrio","doi":"10.1387/ijdb.210005rb","DOIUrl":"https://doi.org/10.1387/ijdb.210005rb","url":null,"abstract":"<p><p>The Spanish Society for Developmental Biology (SEBD) organized its 17th meeting in November 2020 (herein referred to as SEBD2020). This meeting, originally programmed to take place in the city of Bilbao, was forced onto an online format due to the SARS-CoV2, COVID-19 pandemic. Although, we missed the live personal interactions and missed out on the Bilbao social scene, we were able to meet online to present our work and discuss our latest results. An overview of the activities that took place around the meeting, the different scientific sessions and the speakers involved are presented here. The pros and cons of virtual meetings are discussed.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38865461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoreceptor progenitor dynamics in the zebrafish embryo retina and its modulation by primary cilia and N-cadherin.","authors":"Gonzalo Aparicio,&nbsp;Magela Rodao,&nbsp;José L Badano,&nbsp;Flavio R Zolessi","doi":"10.1387/ijdb.200113fz","DOIUrl":"https://doi.org/10.1387/ijdb.200113fz","url":null,"abstract":"<p><p>Photoreceptor cells of the vertebrate neural retina originate in the neuroepithelium, and like other neurons, must undergo cell body translocation and polarity transitions to acquire their final functional morphology, which includes features of neuronal and epithelial cells. We analyzed this process in detail in zebrafish embryos using <i>in vivo</i> confocal microscopy and electron microscopy. Photoreceptor progenitors were labeled by the transgenic expression of enhanced green fluorescent protein under the regulation of the photoreceptor-specific promoter <i>crx</i>, and structures of interest were disrupted using morpholino oligomers to knock-down specific genes. Photoreceptor progenitors detached from the basal retina at pre-mitotic stages, rapidly retracting a short basal process as the cell body translocated apically. They remained at an apical position indefinitely to form the outer nuclear layer (ONL), initially extending and retracting highly dynamic neurite-like processes, tangential to the apical surface. Many photoreceptor progenitors presented a short apical primary cilium. The number and length of these cilia was gradually reduced until nearly disappearing around 60 hpf. Their disruption by knocking-down <i>ift88</i> and <i>elipsa</i> caused a notorious defect on basal process retraction. To assess the role of cell adhesion in the organization of photoreceptor progenitors, we knocked-down <i>cdh2</i>/N-cadherin and observed the cell behavior by time-lapse microscopy. The ectopic photoreceptor progenitors initially migrated in an apparent random manner, profusely extending cell processes, until they encountered other cells to establish cell rosettes in which they stayed, acquiring photoreceptor-like polarity. Altogether, our observations indicate a complex regulation of photoreceptor progenitor dynamics to form the retinal ONL, previous to the post-mitotic maturation stages.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.200113fz","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38380162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental delay during eye morphogenesis underlies optic cup and neurogenesis defects in <i>mab21l2^u517</i> zebrafish mutants.","authors":"Rebecca Wycliffe,&nbsp;Julie Plaisancie,&nbsp;Sydney Leaman,&nbsp;Octavia Santis,&nbsp;Lisa Tucker,&nbsp;Daniela Cavieres,&nbsp;Michelle Fernandez,&nbsp;Camila Weiss-Garrido,&nbsp;Cristian Sobarzo,&nbsp;Gaia Gestri,&nbsp;Leonardo E Valdivia","doi":"10.1387/ijdb.200173lv","DOIUrl":"https://doi.org/10.1387/ijdb.200173lv","url":null,"abstract":"<p><p>Shaping the vertebrate eye requires evagination of the optic vesicles. These vesicles subsequently fold into optic cups prior to undergoing neurogenesis and allocating a population of late progenitors at the margin of the eye. <i>mab21l2</i> encodes a protein of unknown biological function expressed in the developing optic vesicles, and loss of <i>mab21l2</i> function results in malformed eyes. The bases of these defects are, however, poorly understood. To further study <i>mab21l2</i> we used CRISPR/Cas9 to generate a new zebrafish mutant allele (<i>mab21l2^u517</i>). We characterized eye morphogenesis and neurogenesis upon loss of <i>mab21l2</i> function using tissue/cell-type-specific transgenes and immunostaining, <i>in situ</i> hybridization and bromodeoxyuridine incorporation. <i>mab21l2^u517</i> eyes fail to grow properly and display an excess of progenitors in the ciliary marginal zone. The expression of a transgene reporter for the <i>vsx2</i> gene -a conserved marker for retinal progenitors- was delayed in mutant eyes and accompanied by disruptions in the epithelial folding that fuels optic cup morphogenesis. Mutants also displayed nasal-temporal malformations suggesting asynchronous development along that axis. Consistently, nasal retinal neurogenesis initiated but did not propagate in a timely fashion to the temporal retina. Later in development, mutant retinas did laminate and differentiate. Thus, <i>mab21l2^u517</i> mutants present a complex eye morphogenesis phenotype characterized by an organ-specific developmental delay. We propose that <i>mab21l2</i> facilitates optic cup development with consequences both for timely neurogenesis and allocation of progenitors to the zebrafish ciliary marginal zone. These results confirm and extend previous analyses supporting the role of <i>mab21l2</i> in coordinating morphogenesis and differentiation in developing eyes.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1387/ijdb.200173lv","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38382568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental Biology in Chile: historical perspectives and future challenges.","authors":"Miguel L Concha,&nbsp;Iskra A Signore","doi":"10.1387/ijdb.200181mc","DOIUrl":"https://doi.org/10.1387/ijdb.200181mc","url":null,"abstract":"<p><p>Developmental Biology is a growing discipline in Chile. It started in the 1950s when Luis Izquierdo challenged the traditional descriptive perspective of embryology and comparative anatomy to explore the mechanisms underlying the origin of form. After this initial drive, Claudio Barros, beginning in the late 1960s and Juan Fernández, in the late 1970s, contributed with unique and complementary facets to the early growth of the discipline. In the 1980s, the community of developmental biologists created its first forms of association together with the reproduction biology community, and in 1993 the first international course of developmental biology was organised. During the 1990s and 2000s, a group of young investigators arrived in Chile after postdocs in Europe and the US to build the first research centres of developmental biology, fostering the discipline to an unprecedented level. In the 2010s, as these centres consolidated, a stream of young developmental biologists established new labs at several institutions, expanding the community size and broadening its scope. The recent organisation of developmental biology meetings fostered the sense of community and nurtured the need of formal organisation, setting the bases for the foundation of the Chilean Society for Developmental Biology. Today, the community of developmental biologists is a mix of young and experienced investigators working in a variety of geographical locations, institutions, topics and model organisms. These characteristics are a strength of an active community that is pushing the discipline to the next level, aiming to make it a relevant actor in national and international settings.</p>","PeriodicalId":50329,"journal":{"name":"International Journal of Developmental Biology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38382569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}