International Journal of Medical Microbiology最新文献

{"title":"Prolonged carriage of OXA-244-carbapenemase-producing Escherichia coli complicates epidemiological investigations","authors":"Felix Reichert, Simon Brinkwirth, Niels Pfennigwerth, Sebastian Haller, Lena Sophie Fritsch, Tim Eckmanns, Guido Werner, Sören Gatermann, Jörg B. Hans","doi":"10.1016/j.ijmm.2023.151595","DOIUrl":"https://doi.org/10.1016/j.ijmm.2023.151595","url":null,"abstract":"<p>The rapid increase of OXA-244-producing <em>Escherichia coli</em>, predominantly driven by genetically clustered isolates of sequence type (ST)38, has been observed in at least nine European countries, including Germany. However, the reasons for the spread of OXA-244-producing <em>E. coli</em> remain unclear. Here, we aim to evaluate the possibility of prolonged carriage. We identified a total of six different patients with repeated detection of OXA-244-producing <em>E. coli</em> isolates, which were subjected to both short and long-read whole-genome sequencing (WGS). Besides allelic differences using core genome multilocus sequence typing (cgMLST) analyses, we obtained numbers of single-nucleotide polymorphisms (SNPs) to calculate individual base-pair substitution (BPS) rates. To assess possible re-exposure and risk factors for prolonged carriage, case interviews were conducted. The time between detections ranged from eleven months to more than three years. Initial isolates originated in 3/6 cases from clinical samples, remaining samples from screening, mostly in the inpatient setting. As expected, cgMLST analyses showed low numbers of allelic differences between isolates of each case ranging from 1 to 4, whereas numbers of SNPs were between 2 and 99 (mean = 36), thus clearly highlighting the discrepancy between these different bacterial typing approaches. For five out of six cases, observed BPS rates suggest that patients can be colonized with OXA-244-producing <em>E. coli</em>, including ST38 cluster isolates, for extensively long times. Thus, we may have previously missed the epidemiological link between cases because exposure to OXA-244-producing <em>E. coli</em> could have occurred in a time frame, which has not been evaluated in previous investigations. Our results may help to guide future epidemiological investigations as well as to support the interpretation of genetic diversity of OXA-244-producing <em>E. coli</em>, particularly among ST38 cluster isolates.</p>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"9 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139030086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal and anti-biofilm activities of patchouli alcohol against Candida albicans","authors":"Qiulei Zhang ,&nbsp;Jingxiao Zhang ,&nbsp;Yu Zhang ,&nbsp;Yujie Sui ,&nbsp;Yang Du ,&nbsp;Longfei Yang ,&nbsp;Yongjie Yin","doi":"10.1016/j.ijmm.2023.151596","DOIUrl":"10.1016/j.ijmm.2023.151596","url":null,"abstract":"<div><p>The opportunistic fungal pathogen <em>Candida albicans</em> could cause severe clinical outcomes which could be exacerbated by the scarcity of antifungals. The capacity of <em>C. albicans</em> to form biofilms on medical devices that are hard to eradicate, further deepen the need to develop antifungal agents. In this study, we, for the first time, showed that patchouli alcohol (PA) can inhibit the growth of multiple <em>C. albicans</em> strains, as well as four other <em>Candida</em> species, with MICs of 64 μg/mL and MFCs from 64 to 128 μg/mL. The biofilm formation and development, adhesion, yeast-to-hyphal transition and extracellular polysaccharide of <em>C. albicans</em> can be inhibited by PA in a concentration-dependent manner. Confocal microscopy analyses of cells treated with PA showed that PA can increase the membrane permeability and intracellular reactive oxygen species (ROS) production. In <em>C. elegans</em>, PA did not influence the survival below 64 μg/mL. In this study PA demonstrated antifungal and antibiofilm activity against <em>C. albicans</em> and our results showed the potential of developing PA to fight <em>Candida</em> infections.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"314 ","pages":"Article 151596"},"PeriodicalIF":4.1,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422123000243/pdfft?md5=87e6522f90d3784677943e5fdcd637cd&pid=1-s2.0-S1438422123000243-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138832808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolonged carriage of OXA-244-carbapenemase-producing Escherichia coli complicates epidemiological investigations","authors":"Felix Reichert ,&nbsp;Simon Brinkwirth ,&nbsp;Niels Pfennigwerth ,&nbsp;Sebastian Haller ,&nbsp;Lena Sophie Fritsch ,&nbsp;Tim Eckmanns ,&nbsp;Guido Werner ,&nbsp;Sören Gatermann ,&nbsp;Jörg B. Hans","doi":"10.1016/j.ijmm.2023.151595","DOIUrl":"10.1016/j.ijmm.2023.151595","url":null,"abstract":"<div><p>The rapid increase of OXA-244-producing <em>Escherichia coli</em>, predominantly driven by genetically clustered isolates of sequence type (ST)38, has been observed in at least nine European countries, including Germany. However, the reasons for the spread of OXA-244-producing <em>E. coli</em> remain unclear. Here, we aim to evaluate the possibility of prolonged carriage. We identified a total of six different patients with repeated detection of OXA-244-producing <em>E. coli</em> isolates, which were subjected to both short and long-read whole-genome sequencing (WGS). Besides allelic differences using core genome multilocus sequence typing (cgMLST) analyses, we obtained numbers of single-nucleotide polymorphisms (SNPs) to calculate individual base-pair substitution (BPS) rates. To assess possible re-exposure and risk factors for prolonged carriage, case interviews were conducted. The time between detections ranged from eleven months to more than three years. Initial isolates originated in three+ out of six cases from clinical samples, whereas remaining samples were from screening, mostly in the inpatient setting. As expected, cgMLST analyses showed low numbers of allelic differences between isolates of each case ranging from 1 to 4, whereas numbers of SNPs were between 2 and 99 (mean = 36), thus clearly highlighting the discrepancy between these different bacterial typing approaches. For five out of six cases, observed BPS rates suggest that patients can be colonized with OXA-244-producing <em>E. coli</em>, including ST38 cluster isolates, for extensively long times. Thus, we may have previously missed the epidemiological link between cases because exposure to OXA-244-producing <em>E. coli</em> could have occurred in a time frame, which has not been evaluated in previous investigations. Our results may help to guide future epidemiological investigations as well as to support the interpretation of genetic diversity of OXA-244-producing <em>E. coli</em>, particularly among ST38 cluster isolates.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"314 ","pages":"Article 151595"},"PeriodicalIF":4.1,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422123000231/pdfft?md5=a87c9eb916322b02a19c3f1f71b45702&pid=1-s2.0-S1438422123000231-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139022921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etiology of nosocomial infections in intensive care patients in German hospitals: An analysis of trends between 2008 and 2022","authors":"P. Gastmeier, A. Kola, F. Schwab, Michael Behnke, C. Geffers","doi":"10.1016/j.ijmm.2023.151594","DOIUrl":"https://doi.org/10.1016/j.ijmm.2023.151594","url":null,"abstract":"","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"568 ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139018905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Past and present seroprevalence and disease burden estimates of Toxoplasma gondii infections in Germany: An appreciation of the role of serodiagnostics","authors":"Frank Seeber","doi":"10.1016/j.ijmm.2023.151592","DOIUrl":"https://doi.org/10.1016/j.ijmm.2023.151592","url":null,"abstract":"<div><p>Toxoplasmosis is one of the major foodborne parasitic diseases in Germany, with 49% of its population chronically infected with its causative agent, <em>Toxoplasma gondii</em>. Although the acute disease is usually benign in immunocompetent individuals, it is a threat for immunocompromised patients as well as for fetuses of seronegative mothers. As a result of infection, congenital and ocular toxoplasmosis can have serious lifelong consequences. Here I will highlight the epidemiologic situation, from its past in the two separate parts of Germany, to its unification 30 years ago and up to the present day. The main identified risk factor for infection in Germany is thought to be the consumption of undercooked or raw meat or sausages. However, the relative impact of this risky eating habit as well as that of other risk factors are changing and are discussed and compared to the situation in the Netherlands. Finally, the importance of robust and efficient high-throughput serological assays for obtaining reliable epidemiological data, on which public health decisions can be made, is highlighted. The potential of bead-based multiplex assays, which allow the incorporation of multiple antigens with different analytical properties and thus yield additional information, are described in this context. It illustrates the interdependence of new analytic assay developments and sound epidemiology, a foundation that decades-old data from Germany did not have.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151592"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422123000206/pdfft?md5=907cdc86be0677b0e11c90c95b37d8e6&pid=1-s2.0-S1438422123000206-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138484817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interdisciplinary studies on Coxiella burnetii: From molecular to cellular, to host, to one health research","authors":"Benjamin U. Bauer ,&nbsp;Michael R. Knittler ,&nbsp;Jennifer Andrack ,&nbsp;Christian Berens ,&nbsp;Amely Campe ,&nbsp;Bahne Christiansen ,&nbsp;Akinyemi M. Fasemore ,&nbsp;Silke F. Fischer ,&nbsp;Martin Ganter ,&nbsp;Sophia Körner ,&nbsp;Gustavo R. Makert ,&nbsp;Svea Matthiesen ,&nbsp;Katja Mertens-Scholz ,&nbsp;Sven Rinkel ,&nbsp;Martin Runge ,&nbsp;Jan Schulze-Luehrmann ,&nbsp;Sebastian Ulbert ,&nbsp;Fenja Winter ,&nbsp;Dimitrios Frangoulidis ,&nbsp;Anja Lührmann","doi":"10.1016/j.ijmm.2023.151590","DOIUrl":"https://doi.org/10.1016/j.ijmm.2023.151590","url":null,"abstract":"<div><p>The Q-GAPS (Q fever GermAn interdisciplinary Program for reSearch) consortium was launched in 2017 as a German consortium of more than 20 scientists with exceptional expertise, competence, and substantial knowledge in the field of the Q fever pathogen <em>Coxiella</em> (<em>C</em>.) <em>burnetii</em>. <em>C. burnetii</em> exemplifies as a zoonotic pathogen the challenges of zoonotic disease control and prophylaxis in human, animal, and environmental settings in a One Health approach. An interdisciplinary approach to studying the pathogen is essential to address unresolved questions about the epidemiology, immunology, pathogenesis, surveillance, and control of <em>C. burnetii</em>. In more than five years, Q-GAPS has provided new insights into pathogenicity and interaction with host defense mechanisms. The consortium has also investigated vaccine efficacy and application in animal reservoirs and identified expanded phenotypic and genotypic characteristics of <em>C. burnetii</em> and their epidemiological significance. In addition, conceptual principles for controlling, surveilling, and preventing zoonotic Q fever infections were developed and prepared for specific target groups. All findings have been continuously integrated into a Web-based, interactive, freely accessible knowledge and information platform (<span>www.q-gaps.de</span><svg><path></path></svg>), which also contains Q fever guidelines to support public health institutions in controlling and preventing Q fever. In this review, we will summarize our results and show an example of how an interdisciplinary consortium provides knowledge and better tools to control a zoonotic pathogen at the national level.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151590"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422123000188/pdfft?md5=b5ce7695402ae66210e9e93ff83c046a&pid=1-s2.0-S1438422123000188-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138489813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ListiWiki: A database for the foodborne pathogen Listeria monocytogenes","authors":"Christoph Elfmann ,&nbsp;Bingyao Zhu ,&nbsp;Jörg Stülke ,&nbsp;Sven Halbedel","doi":"10.1016/j.ijmm.2023.151591","DOIUrl":"https://doi.org/10.1016/j.ijmm.2023.151591","url":null,"abstract":"<div><p><em>Listeria monocytogenes</em> is a Gram positive foodborne pathogen that regularly causes outbreaks of systemic infectious diseases. The bacterium maintains a facultative intracellular lifestyle; it thrives under a variety of environmental conditions and is able to infect human host cells. <em>L. monocytogenes</em> is genetically tractable and therefore has become an attractive model system to study the mechanisms employed by facultative intracellular bacteria to invade eukaryotic cells and to replicate in their cytoplasm. Besides its importance for basic research, <em>L. monocytogenes</em> also serves as a paradigmatic pathogen in genomic epidemiology, where the relative stability of its genome facilitates successful outbreak detection and elucidation of transmission chains in genomic pathogen surveillance systems. In both terms, it is necessary to keep the annotation of the <em>L. monocytogenes</em> genome up to date. Therefore, we have created the database <em>Listi</em>Wiki (http://listiwiki.uni-goettingen.de/) which stores comprehensive information on the widely used <em>L. monocytogenes</em> reference strain EDG-e. <em>Listi</em>Wiki is designed to collect information on genes, proteins and RNAs and their relevant functional characteristics, but also further information such as mutant phenotypes, available biological material, and publications. In its present form, <em>Listi</em>Wiki combines the most recent annotation of the EDG-e genome with published data on gene essentiality, gene expression and subcellular protein localization. <em>Listi</em>Wiki also predicts protein-protein interactions networks based on protein homology to <em>Bacillus subtilis</em> proteins, for which detailed interaction maps have been compiled in the sibling database <em>Subti</em>Wiki. Furthermore, crystallographic information of proteins is made accessible through integration of Protein Structure Database codes and AlphaFold structure predictions. <em>Listi</em>Wiki is an easy-to-use web interface that has been developed with a focus on an intuitive access to all information. Use of <em>Listi</em>Wiki is free of charge and its content can be edited by all members of the scientific community after registration. In our labs, <em>Listi</em>Wiki has already become an important and easy to use tool to quickly access genome annotation details that we can keep updated with advancing knowledge. It also might be useful to promote the comprehensive understanding of the physiology and virulence of an important human pathogen.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151591"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S143842212300019X/pdfft?md5=84ba6edb81061c002eeb42efac77a618&pid=1-s2.0-S143842212300019X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138474161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicenter evaluation of BioFire JI panel related to improved microbiological diagnostics on acute osteoarticular infections","authors":"Llanos Salar-Vidal ,&nbsp;Catarina Chaves ,&nbsp;Ileana T. Dianzo-Delgado ,&nbsp;Patricio Favier ,&nbsp;Salvador Giner-Almaraz ,&nbsp;María José Gómez-Gómez ,&nbsp;Guillermo Martín-Gutiérrez ,&nbsp;Isabel Pereira ,&nbsp;Ana Rodríguez-Fernández ,&nbsp;Patricia Ruiz-Garbajosa ,&nbsp;Carlos Salas-Venero ,&nbsp;Jaime Esteban","doi":"10.1016/j.ijmm.2023.151588","DOIUrl":"10.1016/j.ijmm.2023.151588","url":null,"abstract":"<div><p>Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms were detected, and fourteen samples were positive for more than one microorganism. In monomicrobial infections (n = 87) agreement with culture was 69%. In 26 samples, the multiplex PCR yield an additional positive result when culture result was negative. It helped in the detection of fastidious microorganisms as <em>K. kingae</em> and <em>N. gonorrhoeae</em>. This multiplex PCR has proven to be a useful technique that can be used for patients with high suspicion of acute OI in a rapid and automated manner.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151588"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71488264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between hospital onset of infection and outcomes in sepsis patients – A propensity score matched cohort study based on health claims data in Germany","authors":"Norman Rose ,&nbsp;Melissa Spoden ,&nbsp;Antje Freytag ,&nbsp;Mathias Pletz ,&nbsp;Tim Eckmanns ,&nbsp;Lisa Wedekind ,&nbsp;Josephine Storch ,&nbsp;Peter Schlattmann ,&nbsp;Christiane S. Hartog ,&nbsp;Konrad Reinhart ,&nbsp;Christian Günster ,&nbsp;Carolin Fleischmann-Struzek","doi":"10.1016/j.ijmm.2023.151593","DOIUrl":"10.1016/j.ijmm.2023.151593","url":null,"abstract":"<div><h3>Background</h3><p>Hospital-acquired infections are a common source of sepsis. Hospital onset of sepsis was found to be associated with higher acute mortality and hospital costs, yet its impact on long-term patient-relevant outcomes and costs is unknown.</p></div><div><h3>Objective</h3><p>We aimed to assess the association between sepsis origin and acute and long-term outcomes based on a nationwide population-based cohort of sepsis patients in Germany.</p></div><div><h3>Methods</h3><p>This retrospective cohort study used nationwide health claims data from 23 million health insurance beneficiaries. Sepsis patients with hospital-acquired infections (HAI) were identified by ICD-10-codes in a cohort of adult patients with hospital-treated sepsis between 2013 and 2014. Cases without these ICD-10-codes were considered as sepsis cases with community-acquired infection (CAI) and were matched with HAI sepsis patients by propensity score matching. Outcomes included in-hospital/12-month mortality and costs, as well as readmissions and nursing care dependency until 12 months postsepsis.</p></div><div><h3>Results</h3><p>We matched 33,110 HAI sepsis patients with 28,614 CAI sepsis patients and 22,234 HAI sepsis hospital survivors with 19,364 CAI sepsis hospital survivors. HAI sepsis patients had a higher hospital mortality than CAI sepsis patients (32.8% vs. 25.4%, RR 1.3, p &lt; .001). Similarly, 12-months postacute mortality was higher (37.2% vs. 30.1%, RR=1.2, p &lt; .001). Hospital and 12-month health care costs were 178% and 22% higher in HAI patients than in CAI patients, respectively. Twelve months postsepsis, HAI sepsis survivors were more often newly dependent on nursing care (33.4% vs. 24.0%, RR=1.4, p &lt; .001) and experienced 5% more hospital readmissions (mean number of readmissions: 2.1 vs. 2.0, p &lt; .001).</p></div><div><h3>Conclusions</h3><p>HAI sepsis patients face an increased risk of adverse outcomes both during the acute sepsis episode and in the long-term. Measures to prevent HAI and its progression into sepsis may be an opportunity to mitigate the burden of long-term impairments and costs of sepsis, e.g., by early detection of HAI progressing into sepsis, particularly in normal wards; adequate sepsis management and adherence to sepsis bundles in hospital-acquired sepsis; and an improved infection prevention and control.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151593"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422123000218/pdfft?md5=cc7369e9bf20126c308b4a6ce79bc5c4&pid=1-s2.0-S1438422123000218-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138529361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of resveratrol on macrophages after phagocytosis of Candida glabrata","authors":"Zong-Han Chen ,&nbsp;Meng Guan ,&nbsp;Wei-Jia Zhao","doi":"10.1016/j.ijmm.2023.151589","DOIUrl":"10.1016/j.ijmm.2023.151589","url":null,"abstract":"<div><p><em>Candida glabrata</em> is believed to be the underlying cause of many human ailments, including oral, gastrointestinal, and vaginal disorders. <em>C. glabrata</em>-caused deep-seated infections, coupled with its resistance to antifungal drugs, may contribute to a high mortality rate. Resveratrol is a polyphenol and can achieve better therapeutic effects when administered in combination with micafungin, but the underlying molecular mechanisms remain unknown. Here, we investigate the effects of varying doses of resveratrol on the proliferation, apoptosis, and activity of macrophages, which were co-cultured with micafungin-pretreated <em>C. glabrata</em>. Resveratrol can restore the decreased proliferative activity of macrophages caused by the phagocytosis of <em>C. glabrata</em>. Further investigations demonstrated that this restoration ability exhibited a dose-dependent manner, reaching the highest level at 200 µM of resveratrol. Resveratrol tended to be more effective in inhibiting macrophage apoptosis and reducing reactive oxygen species (ROS) levels with concentration increases. In addition, at medium concentrations, resveratrol may down-regulate the expression of most inflammatory cytokines, whereas at high concentrations, it started to exert pro-inflammatory functions by up-regulating their expressions. Macrophages may shift from an anti-inflammatory (M2) phenotype to an inflammatory (M1) phenotype by resveratrol at 200 µM, and from M1 to M2 at 400 µM. Our research shows that resveratrol with micafungin are effective in treating <em>C. glabrata</em> infections. The resveratrol-micafungin combination can reduce the production of ROS, and promote the proliferation, inhibit the apoptosis, and activate the polarization of macrophages in a dose-dependent manner. This study offers insights into how this combination works and may provide possible direction for further clinical application of the combination.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 6","pages":"Article 151589"},"PeriodicalIF":4.1,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422123000176/pdfft?md5=10c10801df9fb875840599bbe8fa302a&pid=1-s2.0-S1438422123000176-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89720367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}