Identification and characterization of a novel α-haemolytic streptococci, Streptococcus parapneumoniae sp. nov., which caused bacteremia with pyelonephritis

IF 4.5 3区医学 Q1 MICROBIOLOGY

International Journal of Medical Microbiology Pub Date : 2024-06-01 DOI:10.1016/j.ijmm.2024.151625

Yuri Katayama , Masatomo Morita , Bin Chang , Daisuke Katagiri , Masahiro Ishikane , Gen Yamada , Kazuhisa Mezaki , Masami Kurokawa , Hideki Takano , Yukihiro Akeda

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引用次数: 0

Abstract

Objectives

We report a case of bacteremia with pyelonephritis in an adult male with an underlying disease caused by α-hemolytic streptococci. α-Hemolytic streptococci were isolated from blood, but it was challenging to identify its species. This study aimed to characterize the causative bacterium SP4011 and to elucidate its species.

Methods

The whole-genome sequence and biochemical characteristics of SP4011 were determined. Based on the genome sequence, phylogenetic analysis was performed with standard strains of each species of α-hemolytic streptococci. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values were calculated.

Results

SP4011 showed optochin susceptibility and bile solubility, but did not react with pneumococcal omni antiserum. Phylogenetic analysis of the whole-genome sequence showed that SP4011 clustered with S. pneumoniae and S. pseodopneumoniae and was most closely related to S. pseodopneumoniae. Genomic analysis revealed that ANI and dDDH values between SP4011 and S. pseodopneumoniae were 94.0 % and 56.0 %, respectively, and between SP4011 and S. pneumoniae were 93.3 % and 52.2 %, respectively. Biochemical characteristics also showed differences between SP4011 and S. pseodopneumoniae and between SP4011 and S. pneumoniae. These results indicate that SP4011 is a novel species.

Conclusion

Our findings indicate that SP4011 is a novel species of the genus Streptococcus. SP4011 has biochemical characteristics similar to S. pneumoniae, making it challenging to differentiate and requiring careful clinical diagnosis. This isolate was proposed to be a novel species, Streptococcus parapneumoniae sp. nov. The strain type is SP4011^T (= JCM 36068^T = KCTC 21228^T).

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新型α-溶血性链球菌--副肺炎链球菌新菌株的鉴定和特征描述，该菌株可引起伴有肾盂肾炎的菌血症

我们报告了一例由α-溶血性链球菌引起的伴有肾盂肾炎的成年男性菌血症。本研究旨在描述致病菌 SP4011 的特征，并阐明其种类。研究测定了 SP4011 的全基因组序列和生化特征。根据基因组序列，对α-溶血性链球菌各菌种的标准菌株进行了系统发育分析。计算了数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）值。SP4011 对光素有敏感性，并具有胆汁溶解性，但与肺炎球菌全能抗血清没有反应。全基因组序列的系统进化分析表明，SP4011 与和和的亲缘关系最密切。基因组分析表明，SP4011 与之间的 ANI 值和 dDDH 值分别为 94.0 % 和 56.0 %，SP4011 与之间的 ANI 值和 dDDH 值分别为 93.3 % 和 52.2 %。生化特征也显示出 SP4011 和 SP4011 之间以及 SP4011 和 SP4011 之间的差异。这些结果表明，SP4011 是一个新物种。我们的研究结果表明，SP4011 是.属的一个新物种。SP4011 的生化特征与Ⅳ相似，因此很难将其区分开来，需要进行仔细的临床诊断。该分离株被认为是一个新菌种（sp. nov.菌株类型为 SP4011（= JCM 36068 = KCTC 21228）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Medical Microbiology 医学-病毒学

CiteScore

9.70

自引率

0.00%

发文量

审稿时长

45 days

期刊介绍： Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.