Journal of Experimental Marine Biology and Ecology最新文献

{"title":"Resilience to changes in salinity and predator diversity in representative Gulf of Mexico estuarine fish predator-prey interactions","authors":"Scott B. Alford ,&nbsp;Shea A. Husband ,&nbsp;Charles W. Martin","doi":"10.1016/j.jembe.2024.152053","DOIUrl":"10.1016/j.jembe.2024.152053","url":null,"abstract":"<div><p>Predators strongly influence ecological communities and subsequent ecosystem function by directly consuming prey individuals and indirectly by inciting alterations in prey behavior, termed non-consumptive effects (NCEs). Gulf of Mexico estuaries support complex food webs structured by numerous species interactions maintained in constant environmental flux from variation in salinity. Environmental variation presents a source of physiological stress for estuarine fish and plays a role in their distributions and subsequent interactions. Here, we present the results of a mesocosm experiment examining if acute changes in salinity disrupt NCEs of different predators on estuarine prey. We exposed Gulf killifish (<em>Fundulus grandis</em>) to visual and/or olfactory cues from a common estuarine predator, red drum (<em>Sciaenops ocellatus</em>), and from a freshwater predator that is a transient species in lower portions of estuaries, largemouth bass (<em>Micropterus salmoides</em>), under either decreasing, constant, or increasing salinity. Killifish decreased shoal size and movement in response to olfactory cues or both visual and olfactory cues compared to when they were exposed to no cues or visual cues, demonstrating variable perceived risk dependent on cue type. Behavioral response did not vary by predator species, implying a generalized response to different fish predators. Prey did not change behavior when exposed to decreasing salinity compared to constant salinity. However, prey movement increased with increasing salinity, indicating that salinity changes may provide cues to search for different habitats instead of a stressor. Our experiment indicates that estuarine predator-prey interactions may be resilient to acute salinity changes given the broad tolerances of euryhaline species.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"580 ","pages":"Article 152053"},"PeriodicalIF":1.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes of glycolysis and gluconeogenesis key enzymes in the muscle of the shrimp Penaeus vannamei in response to hypoxia and reoxygenation","authors":"Dalia G. Cruz-Moreno ,&nbsp;Laura E. Hernández-Aguirre ,&nbsp;Alma B. Peregrino-Uriarte ,&nbsp;Lilia Leyva-Carrillo ,&nbsp;Silvia Gómez-Jiménez ,&nbsp;Carmen Contreras-Vergara ,&nbsp;Jorge Hernández-López ,&nbsp;Gloria Yepiz-Plascencia","doi":"10.1016/j.jembe.2024.152052","DOIUrl":"10.1016/j.jembe.2024.152052","url":null,"abstract":"<div><p>Although the shrimp <em>Penaeus vannamei</em> can tolerate oxygen-limited conditions, the organisms may suffer detrimental effects in several processes, including growth, reproduction, immune responses, and others. During hypoxia, the shrimp makes metabolic adjustments in energy use and glucose homeostasis. Glucose is hydrolyzed during glycolysis and synthesized from non-glycosidic metabolites in gluconeogenesis. Although these pathways share many enzymes, the processes are not exactly the reverse of each other. We studied the first and last irreversible steps of glycolysis and gluconeogenesis catalyzed by hexokinase/glucose-6-phosphatase (HK/G6Pase) and pyruvate kinase/pyruvate carboxylase/phosphoenolpyruvate carboxykinase (PK/PC/PEPCK), respectively, in muscle under hypoxia and hypoxia followed by reoxygenation to gain insights about the coordinated metabolic responses that maintain glucose balance during oxygen stress. For the first group, HK gene expression was induced in hypoxia while G6Pase was reduced in hypoxia and reoxygenation, whereas for enzymes activity, HK was maintained and G6Pase was only detected in reoxygenation. For the second group, gene expression increased in PK in hypoxia, in PC in reoxygenation and in both cases, the enzymatic activities were maintained. Mitochondrial PEPCK expression increased during hypoxia and cytosolic PEPCK decreased, while total PEPCK activity was maintained. Intracellular lactate decreased during hypoxia while glucose levels were maintained. Altogether the results show that in limited oxygen, the expression of glycolytic enzymes-genes are induced, the gluconeogenic counterparts are active, although with more varied responses to oxygen limited conditions, and intracellular glucose levels are maintained.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"580 ","pages":"Article 152052"},"PeriodicalIF":1.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial DNA damage, repair and copy number dynamics of Sclerophytum sp. (Anthozoa: Octocorallia) in response to short-term abiotic oxidative stress","authors":"Gaurav G. Shimpi ,&nbsp;Sergio Vargas ,&nbsp;Bastian Bentlage ,&nbsp;Gert Wörheide","doi":"10.1016/j.jembe.2024.152051","DOIUrl":"10.1016/j.jembe.2024.152051","url":null,"abstract":"<div><p>As a consequence of global climate change, the increasing frequency of environmental disturbances and surplus oxidative stress experienced by coral reefs will likely contribute to phase shifts from stony to soft corals. Mitochondrial response to reactive oxygen species (ROS) -induced oxidative damage appears pivotal for bioenergetic adaptation and recovery during environmental stress, partly governed by mitochondrial DNA copy number. Unlike other animals, octocorals possess unique mitogenomes with an intrinsic DNA mismatch repair gene, the <em>mtMutS,</em> that is likely to have a role in mitochondrial response and mtDNA damage recovery. Yet, there is a general lack of stress response studies on octocorals from a mitochondrial perspective. Here we evaluate the mitochondrial response of the octocoral <em>Sclerophytum</em> sp. subjected to acute elevated temperature and low pH, and its putative competence to reverse oxidative mtDNA damage caused by exogenous agents like hydrogen peroxide (H₂O₂). Temporal changes in mtDNA copy number and mtDNA damage and recovery were monitored. Both short-term thermal and low pH stress applied independently instigated mtDNA damage and affected mtDNA copy number differently, while <em>mtMutS</em> gene was significantly upregulated during low pH stress. mtDNA damage caused by H₂O₂ insult was observed to be promptly reversed in <em>Sclerophytum</em> sp., and a higher mtDNA copy number was associated with lower mtDNA damage. These findings provide insights into the potential role of <em>mtMutS</em> gene in conferring resilience to octocorals, the relevance of mtDNA copy number, and emphasize the importance of better understanding the mitochondrial stress response of cnidarians in the context of climate change.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"580 ","pages":"Article 152051"},"PeriodicalIF":1.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022098124000662/pdfft?md5=6f990cd20c0afe302442496a4eee1c0a&pid=1-s2.0-S0022098124000662-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142039798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental and biotic habitat attributes affect rocky intertidal community variability in glacially influenced estuaries","authors":"Madeleine McArthur ,&nbsp;Schery Umanzor ,&nbsp;Julie Schram ,&nbsp;Brenda Konar","doi":"10.1016/j.jembe.2024.152050","DOIUrl":"10.1016/j.jembe.2024.152050","url":null,"abstract":"&lt;div&gt;&lt;p&gt;Climate change is increasing the rate of glacial recession in high latitude coastal environments. The associated increase in cold, sediment-laden freshwater into the nearshore may alter a wide array of water attributes, which will likely have ecosystem-wide impacts. One of these potential impacts is a change in typical patterns of ecological community variability, which can be an indicator of stress in response to an environmental impact. Fluctuation in community composition over multiple temporal scales is natural and can be affected by environmental conditions, as well as by the presence of habitat forming, spatially dominant organisms. The goal of this study was to determine how variation in environmental attributes and spatially dominant species contributes to the seasonal variability of rocky intertidal community assemblages in glacially influenced estuaries. The environmental attributes of interest were both dynamic in nature (water temperature, salinity, dissolved oxygen, turbidity, and pCO&lt;sub&gt;2)&lt;/sub&gt;, and static (substrate type, wave exposure, beach slope, and distance to freshwater). Variation in the proportions of three spatially dominant organisms that are common in many intertidal systems, &lt;em&gt;Mytilus trossulus&lt;/em&gt; (mussels), &lt;em&gt;Balanus&lt;/em&gt; spp. (barnacles), and &lt;em&gt;Fucus&lt;/em&gt; spp. (rockweed) were examined as biotic habitat attributes. It was hypothesized that 1) dynamic environmental attributes would be more strongly correlated to community variability patterns than static attributes, and 2) higher proportions of spatially dominant species would correspond to lower levels of community variability. To document seasonal community variability, as well as proportions of spatially dominant species, percent cover and biomass data were collected along with environmental data from 2019 to 2022. Barnacle cover, rockweed cover, and substrate characteristics (cover of gravel and total cover of bare rock) were significantly correlated to community variability levels based on percent cover data. Community variability levels based on biomass data were significantly correlated to mussel cover, rockweed biomass, substrate aspects (cover of gravel and mud), distance to a freshwater source, and variation in dissolved oxygen levels. Almost all relationships between community variability and each of these attributes were negative, where a greater level of an environmental attribute was associated with lower seasonal variability in community composition. Distance to freshwater and dissolved oxygen variation had a positive relationship to community variability, meaning that greater distance to a freshwater source and variation in dissolved oxygen were associated with higher seasonal community variability. As several of these variability drivers may be affected by climate-related changes, the results of this study provide insights into how glacially influenced rocky intertidal communities may in turn be affected by the progression of climate c","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"580 ","pages":"Article 152050"},"PeriodicalIF":1.8,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Garibaldi (Hypsypops rubicundus) from artificial reefs and cooler island natural reefs consume more animal material, which is associated with greater adult size and body condition","authors":"Brenda E. Fuentes Calderon ,&nbsp;Jacob L. Eagleton ,&nbsp;Chelsea M. Williams ,&nbsp;Jeremy T. Claisse","doi":"10.1016/j.jembe.2024.152048","DOIUrl":"10.1016/j.jembe.2024.152048","url":null,"abstract":"<div><p>Access to and quality of dietary resources can affect marine fish life history metrics as ingested prey are transformed into the energy and nutrients used for growth and life-sustaining activities. Here, we analyze the diet composition of Garibaldi (<em>Hypsypops rubicundus</em>) populations across natural and artificial rocky reefs subject to a wide range of environmental conditions within the Southern California Bight (SCB). The diet of populations from the warmer islands and mainland natural reefs had higher proportions of algae, while the diet of populations from cooler island natural reefs and mainland artificial reefs had higher proportions of animal content. More animal material in the diet was positively associated with higher body condition in both sexes, larger adult average length, and higher initial growth rates, with relationships generally being stronger in females. These results support the role of the habitat-related variation in diet as a driver of local differences in growth patterns and body condition and provide a more comprehensive description of Garibaldi's omnivorous diet. These findings also provide implications for performance assessments of artificial reef habitats.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"580 ","pages":"Article 152048"},"PeriodicalIF":1.8,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022098124000637/pdfft?md5=e7668532ba185df021b3b091852ae950&pid=1-s2.0-S0022098124000637-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141964573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regional differences in the effects of watershed glacial coverage on the performance of Pacific Blue mussel, Mytilus trossulus","authors":"Gracelyn Ham ,&nbsp;Scott Gabara ,&nbsp;Preslee Chase ,&nbsp;Brenda Konar ,&nbsp;Schery Umanzor","doi":"10.1016/j.jembe.2024.152043","DOIUrl":"10.1016/j.jembe.2024.152043","url":null,"abstract":"<div><p>Climate change is causing atmospheric warming, leading to increasing global temperatures. Warming is especially evident in high latitude regions, leading to losses in glacial mass and consequences downstream. Pacific Blue mussels are a vital species in such downstream coastal environments where they provide services such as stabilizing substrate, facilitating energy transfer, and supporting the mariculture industry and subsistence communities. As glaciers continue to melt, it is unknown how and to what extent the projected glacial runoff will affect intertidal mussel performance. To determine the effects of glacial melt on mussel performance, mussel morphometrics (weight, length, width), mussel adhesion force, shell breaking force, and growth rates were recorded over five months at nine sites whose watersheds span a gradient of glacial coverage (0–60%) in the Gulf of Alaska (GOA). Five sites were used in Kachemak Bay (Southcentral Alaska) and four sites in Lynn Canal (Southeast Alaska). Overall, adhesion force and shell breaking force decreased with increasing glacial coverage of watersheds. However, oceanic proximity and input appear to buffer against the effects of glacial melt as mussels in the more oceanic Kachemak Bay region had higher adhesion and shell breaking forces relative to the mussels in the less oceanic Lynn Canal region. Within the more oceanic Kachemak Bay region, mussel growth rates decreased with increasing glacial coverage of watersheds. In contrast, mussel growth rates generally increased at sites with increasing glaciation of watersheds in the less oceanic Lynn Canal region. Overall, our results suggest that glacial melt is altering conditions in the nearshore marine environment and reducing mussel performance.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"580 ","pages":"Article 152043"},"PeriodicalIF":1.8,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022098124000583/pdfft?md5=043f23d7a9d6e4570477cab32da794ce&pid=1-s2.0-S0022098124000583-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141979348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coupling effects of CO2-induced ocean acidification and nitrogen enrichment on growth, photosynthesis and nitrogen utilization of an invasive seaweed (Sargassum muticum)","authors":"Fang Yan ,&nbsp;Zhengzheng Lv ,&nbsp;Huichao Jiang ,&nbsp;Hongyan Wu ,&nbsp;Xiukai Song ,&nbsp;Xiangyang Jiang ,&nbsp;Chen Gao ,&nbsp;Menglin Bao ,&nbsp;Shasha Zang ,&nbsp;Zhiguang Xu ,&nbsp;Lijuan Liu","doi":"10.1016/j.jembe.2024.152049","DOIUrl":"10.1016/j.jembe.2024.152049","url":null,"abstract":"<div><p><em>Sargassum muticum</em>, an invasive seaweed, has colonized many parts of the world along the coast. Marine environment invaded by this species is aggravated the complexity by CO₂-induced ocean acidification (OA) and coastal eutrophication. However, the coupling effects of seawater acidification and eutrophication on this invasive species remain unclear. In this study, we cultured <em>Sargassum muticum</em> at two concentrations of <em>p</em>CO₂ (420 ppmv, LC and 1000 ppmv, HC) and nitrate (10 μM, LN and 200 μM, HN) for 16 days, to investigate the coupling effects of CO₂-induced seawater acidification and nitrate enrichment on growth and photosynthesis of <em>Sargassum muticum</em>. The results showed that high CO₂ increased the relative growth rate (RGR) of alga by 58.9% under LN condition, while such increment was not found under HN condition. Thus, the highest RGR was emerged in the HCLN treatment. The photosynthetic rate curve under different inorganic carbon concentrations (P<img>C curve) presented that high CO₂ increased the maximum inorganic carbon utilization rate (<em>V</em>_max) by 8.1% under HN condition; while inhibited it by 29.8% under LN condition. The affinity to inorganic carbon, reflected by the half-saturation constant (<em>K</em>_0.5), was improved significantly by high CO₂ and/or high nitrate, compared with LCLN treatment. The photosynthetic rate curves under different irradiances (P<img>I curve) suggested that the maximum photosynthetic rate (<em>P</em>_max) of alga was enhanced remarkably by high N, and kept unaffected by high CO₂. The lowest value of dark respiration rate (<em>R</em>_d) was found in HCLN treatment, and there was no significant difference among the other three treatments. Additionally, an increase chlorophyll <em>a</em> content caused by high N was only found in HC treatment. After 16 d culture, nitrate reductase activity (NRA) of algae in HN treatments decreased significantly, compared with those in LN treatments. Furthermore, high CO₂ enhanced NRA dramatically only in algae grown at LN level. Correspondingly, the lowest nitrate uptake rate (NUR) was found in LCHN treatment, and there was no significant difference among the other three treatments. In conclusion, our results showed that elevated CO₂ enhanced the RGR, and the coupling of high CO₂ and nitrate affected the photosynthesis and NUR, however did not synergistically promote growth of <em>S. muticum</em>. Therefore, we speculate that the future OA may exacerbate the invasiveness of <em>S. muticum</em>; nevertheless, the eutrophication of seawater would not amplify this effect.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"579 ","pages":"Article 152049"},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141933884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal dynamics of microbial communities in the water of polyculture pond system for Chinese swimming crab Portunus trituberculatus","authors":"Qian Huang ,&nbsp;Meng Li ,&nbsp;Sha Xu ,&nbsp;Caiwen Li","doi":"10.1016/j.jembe.2024.152047","DOIUrl":"10.1016/j.jembe.2024.152047","url":null,"abstract":"<div><p>Microorganisms as the vital component of aquaculture ecosystems, were involved in nutrient cycling, water quality regulation, and the health of cultured species. In this study, aquatic water samples were collected monthly to reveal microbial community compositions, functions, and interactions in different habitats (pond and waterway) during the culture stages of the Chinese swimming crab <em>Portunus trituberculatus</em> in Shandong Province. Due to the semi-closed conditions with limited water exchange, the <em>α</em> diversities were lower in the polyculture pond. The relative abundances of the dominant phyla varied dramatically, and most biomarkers were identified in the polyculture pond. The <em>β</em> diversity of the microbial communities significantly differed among culture stages and habitats. Furthermore, the heterogeneity of prokaryotic microorganisms was greater than that of eukaryotic microorganisms. In the polyculture pond, the microbial community was sensitive to nutrient cycling, with abundant functions related to “photoautotrophy”, “nitrogen_respiration” and “nitrate_respiration”, corresponding to <em>P. trituberculatus</em> culture activities. The <em>co</em>-occurrence network of the microbial community in the polyculture pond tended to be looser than that in the waterway, with a lower number of taxa and correlations. In addition, these networks had nonrandom patterns and formed “small world” topological structures. The low-abundance genera with a higher level of betweenness centrality scores were the hubs or connectors in the networks and might play an essential role in microbial community composition. Furthermore, potential pathogens (<em>Vibrio</em>, <em>Acholeplasma</em>, <em>Photobacterium</em>, and <em>Flavobacterium</em>) deserve more attention during <em>P. trituberculatus</em> culture. This study will be useful for understanding microbial communities in polyculture pond systems and will provide a reference for the health aquaculture of <em>P. trituberculatus</em>.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"579 ","pages":"Article 152047"},"PeriodicalIF":1.8,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141933885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lethal and sub-lethal responses of rocky shore gastropods to extreme temperatures","authors":"H.M. Parry-Wilson ,&nbsp;P.B. Fenberg ,&nbsp;S.J. Hawkins ,&nbsp;N. Mieszkowska","doi":"10.1016/j.jembe.2024.152044","DOIUrl":"10.1016/j.jembe.2024.152044","url":null,"abstract":"<div><p>Intertidal communities are more frequently experiencing extreme air and sea temperatures as a result of anthropogenic climate change, with heatwaves increasing in intensity and duration. Most studies exploring the thermal tolerances of intertidal species to extreme temperatures haven't directly studied early life stages. We explored whether locally extreme tide-out temperatures were lethally impacting premature Boreal (<em>Littorina littorea</em> and <em>Steromphala cineraria</em>) and Lustanian (<em>Phorcus lineatus</em> and <em>Steromphala umbilicalis</em>) intertidal gastropod species from southwest England. Two separate experiments tested lethal and sub-lethal responses to simulated local heatwave and cold spell temperatures. Two sensitive early size classes (new recruits and year 1+ juveniles) for each species were exposed to simulated extreme and ambient tide-out temperatures in replicated boulderfield (air) and rockpool (seawater) microhabitats per experiment. Realistic experimental temperatures were determined by EnvLogger temperature data recorded locally in sun-exposed, shaded and rockpool intertidal habitats. For each nine-day experiment, specimens were exposed for six hours daily to four laboratory thermal treatments simulating both ambient conditions and extreme temperature events. These experiments were designed to test the following questions: are premature trochids and littorinids more sensitive to extreme temperatures than adults, and is there a difference in response between species of Boreal and Lusitanian origin sampled from the same geographical region? We identified that the premature intertidal trochid species may not be more thermally sensitive than adults, although premature <em>L. littorina</em> may have a slightly lower lethal heat limit. Survivorship in extreme heatwave air temperatures (40.0–42.9 °C) was lowest for lowshore and highest for highshore species, relating directly to species' vertical shore zonation. There were minimal to zero mortalities in all other thermal treatments. Following stress-induced inactivity in the extreme heatwave air temperature treatment, <em>S. cineraria</em> and <em>P. lineatus</em> specimens all died. In contrast, <em>L. littorea</em> and <em>S. umbilicalis</em> specimens recovered in some instances. Intensifying and more frequent aerial heatwave events occurring unseasonably are likely to cause population declines, local extinctions or subtidal retreat for premature lowshore Boreal intertidal species, influencing recruitment to adult populations, especially where rockpools are not present to provide thermal refugia. Conversely, mid-highshore Lusitanian species are likely to be more resilient to high air temperatures during low tides. Cold spell temperatures did not lethally or sub-lethally impact any juvenile gastropod species, suggesting Lusitanean species will thrive as a result of increasingly milder winters.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"579 ","pages":"Article 152044"},"PeriodicalIF":1.8,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022098124000595/pdfft?md5=031fe50c5ada3befabca42b6ae65db66&pid=1-s2.0-S0022098124000595-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141933949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interacting effects of local and global stressors on mussel beds and ecosystem functioning","authors":"Charlotte Carrier-Belleau ,&nbsp;Félix Lauzon ,&nbsp;Jérémie Boucher-Fontaine ,&nbsp;Scott Tiegs ,&nbsp;Mathieu Cusson ,&nbsp;Frédéric Guichard ,&nbsp;Christian Nozais ,&nbsp;Philippe Archambault","doi":"10.1016/j.jembe.2024.152046","DOIUrl":"10.1016/j.jembe.2024.152046","url":null,"abstract":"<div><p>Biogenic habitats, such as mussel beds, provide various functions in their associated ecosystems. However, these habitat-forming species are exposed to cumulative impacts as the number and diversity of anthropogenic stressors increase, particularly in estuarine ecosystems. Experiments designed to test the effect of single and multiple interacting stressors on mussel beds and associated biotic components are rare (i.e. in situ experiments are uncommon, as they usually occur in laboratory settings). We conducted a field experiment in the St. Lawrence estuary (Québec, Canada) to address this gap. We transplanted blue mussels (<em>Mytilus</em> spp.) to mimic mussel beds and exposed them to increased nutrient concentrations and thermal stress at three intervals (6.5, 10.5 and 15 weeks) during May through September. For each transplant, we evaluated epizoic microalgal biomass (as pigment biomass), microbial activity and oxygen uptake, and mortality levels and energy content in the tissues of <em>Mytilus</em> spp. following three exposure times. No effects were found for chlorophyll <em>a</em> biomass, microbial activity and oxygen uptake, and mortality in mussels. In contrast, we found thermal stress and nutrient input interacted to create antagonistic and synergistic effects on energy content in <em>Mytilus</em> spp. at different exposure times and exerted additive effects over time on phaeopigments and the ratio of chlorophyll <em>a</em>/phaeopigments. Our work highlights the importance of combining multiple biological components (i.e. multiple biological responses measured at different scales of biological complexity) and different experimental approaches to capture the complexity behind stressor interactions.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"579 ","pages":"Article 152046"},"PeriodicalIF":1.8,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022098124000613/pdfft?md5=a25bf0484e1ebbe5ff43b01316187922&pid=1-s2.0-S0022098124000613-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141951441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}