Scandinavian Journal of Clinical and Laboratory Investigation最新文献_第3页

Fecal calprotectin as a biomarker of intestinal inflammation in ICU patients with diarrhea - testing the pipette method against the collection pin and weighing methods. 粪钙保护蛋白作为ICU腹泻患者肠道炎症的生物标志物——移液法与收集针法和称重法的比较

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-10-01 Epub Date: 2022-08-09 DOI: 10.1080/00365513.2022.2107567

Karoline Hardis, Sarah B Johansen, Janne Eriksen, Kjeld Damgaard, Peter Derek Christian Leutscher, Soren Jepsen

{"title":"Fecal calprotectin as a biomarker of intestinal inflammation in ICU patients with diarrhea - testing the pipette method against the collection pin and weighing methods.","authors":"Karoline Hardis, Sarah B Johansen, Janne Eriksen, Kjeld Damgaard, Peter Derek Christian Leutscher, Soren Jepsen","doi":"10.1080/00365513.2022.2107567","DOIUrl":"https://doi.org/10.1080/00365513.2022.2107567","url":null,"abstract":"Fecal calprotectin is an established biomarker in inflammatory bowel disease, but its role in assessment of intestinal inflammation in ICU patients is yet to be explored. ICU patients tend to acquire diarrhea, which complicates the extraction of fecal calprotectin using a collection pin for stool sampling. Pipetting is an alternative sampling method. The aim of the study was to compare the collection pin method with the pipette method for measurement of fecal calprotectin in ICU patients with diarrhea. Stool samples were collected from fecal bags used in the patients and then analyzed for calprotectin using an extraction device and a piston pipette, respectively. In addition, a validation test for the pipette method of extraction was conducted on liquid stool samples. Eleven stool samples were collected from five randomly selected ICU patients. Calprotectin was detectable in all fecal samples. On average the collection pin measured 45% lower than the pipette method with a 95% confidence interval (30 - 59%). The coefficient of variation when using the pipette method was 13% in low calprotectin concentrations and 39% in high concentrations. In conclusion, the pipette method seems to be appropriate for measuring calprotectin in liquid feces, due to practicalities and a greater precision.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"504-507"},"PeriodicalIF":2.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40692645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unusual total anti-SARS-CoV-2 antibody kinetics observed during longitudinal monitoring after BNT162b2 vaccination. 接种BNT162b2疫苗后纵向监测中观察到的异常总抗sars - cov -2抗体动力学

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-10-01 Epub Date: 2022-09-21 DOI: 10.1080/00365513.2022.2123388

Ivana Lapić, Dunja Rogić, Dragana Šegulja, Ana Kozmar, Marta Kmet, Lovorka Đerek, Renata Zadro

{"title":"Unusual total anti-SARS-CoV-2 antibody kinetics observed during longitudinal monitoring after BNT162b2 vaccination.","authors":"Ivana Lapić, Dunja Rogić, Dragana Šegulja, Ana Kozmar, Marta Kmet, Lovorka Đerek, Renata Zadro","doi":"10.1080/00365513.2022.2123388","DOIUrl":"https://doi.org/10.1080/00365513.2022.2123388","url":null,"abstract":"The present study aimed to clarify unusual total antibody kinetics in three female individuals observed during longitudinal monitoring of antibody response to BNT162b2 COVID-19 vaccine in 54 healthy volunteers. Total and IgG antibodies against the SARS-CoV-2 spike glycoprotein were measured using Roche and Abbott quantitative assays, respectively, a day before and 8, 71, 135 and 217 days after the second dose. Samples showing unusual kinetics were additionally tested with Beckman Coulter and Euroimmun IgG assays, as well as IgA assay. Antibody levels peaked 8 days after the second dose (total:2769 U/mL; IgG:20022 AU/mL) and declined to 611 U/mL (total) and 783 AU/mL (IgG), after 217 days. A delayed increase of total but not IgG antibodies evidenced in three females, was in two cases coupled with an increase in IgA antibodies. This study identified a previously unknown contribution of anti-SARS-CoV-2 IgA antibodies to a delayed total antibody increase in a subgroup of vaccinated individuals. It also emphasizes that different commercially available serological assays do not provide uniform information about the post-vaccination immune status and that thorough understanding the assays' features is crucial for the proper interpretation of antibody response monitoring.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"486-491"},"PeriodicalIF":2.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40373915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between fibrosis markers and kidney function following peptide receptor radionuclide therapy in patients with neuroendocrine tumours. 神经内分泌肿瘤患者肽受体放射性核素治疗后纤维化标志物与肾功能的关系。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-10-01 Epub Date: 2022-09-21 DOI: 10.1080/00365513.2022.2119598

Tobias Stemann Lau, Lars Bossen, Daniel Guldager Kring Rasmussen, Morten Karsdal, Federica Genovese, Anne Kirstine Arveschoug, Henning Gronbaek, Gitte Dam

{"title":"Association between fibrosis markers and kidney function following peptide receptor radionuclide therapy in patients with neuroendocrine tumours.","authors":"Tobias Stemann Lau, Lars Bossen, Daniel Guldager Kring Rasmussen, Morten Karsdal, Federica Genovese, Anne Kirstine Arveschoug, Henning Gronbaek, Gitte Dam","doi":"10.1080/00365513.2022.2119598","DOIUrl":"https://doi.org/10.1080/00365513.2022.2119598","url":null,"abstract":"Peptide receptor radionuclide therapy (PRRT) is a treatment for neuroendocrine tumours (NET). Renal impairment is a known side effect due to kidney fibrosis. We investigated the association between novel specific fibrosis markers and kidney function following PRRT. We included 38 patients who had all finished PRRT. In serum and urine, we analysed levels of three different fibrosis markers, PRO-C6 (type VI collagen formation), PRO-C3 (type III collagen formation) and C3M (type III collagen degradation). We determined kidney function by the 51Cr-EDTA plasma clearance. We used Wilcoxon rank sum test and Spearman's rank correlation to evaluate the association between the fibrosis markers and kidney function. We included 38 NET patients, 25 small-intestinal NET, 6 pancreatic NET, 2 pulmonary NET and 5 other types of NET. Median age was 69 years (IQR: 61-73). Median time from last PRRT to inclusion was 8 months (IQR: 3-20). We found significantly increased levels of serum PRO-C6 (p = .007) and urinary PRO-C6 (p = .033) and significantly decreased levels of urinary C3M (p = .035) in patients with impaired kidney function. Further, we observed a negative association between serum PRO-C6 and kidney function (rho = -0.33, p = .04) and a positive association between urinary C3M and kidney function (rho = 0.37, p = .02). We showed an association between the three fibrosis markers, serum PRO-C6, urinary PRO-C6 and urinary C3M and kidney function. These markers may help to improve the understanding of potential pathological tissue turnover and potentially improve monitoring of kidney function after PRRT in NET patients.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"446-453"},"PeriodicalIF":2.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40373914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-10-01 Epub Date: 2022-09-21 DOI: 10.1080/00365513.2022.2122076

Maria Kløjgaard Skytthe, Anna Lahn Sørensen, Dorle Hennig, Maria Boysen Sandberg, Lars Melholt Rasmussen, Peter Højrup, Holger J Møller, Karsten Skjødt, Søren Kragh Moestrup, Jonas Heilskov Graversen

{"title":"Haptoglobin-related protein in human plasma correlates to haptoglobin concentrations and phenotypes.","authors":"Maria Kløjgaard Skytthe, Anna Lahn Sørensen, Dorle Hennig, Maria Boysen Sandberg, Lars Melholt Rasmussen, Peter Højrup, Holger J Møller, Karsten Skjødt, Søren Kragh Moestrup, Jonas Heilskov Graversen","doi":"10.1080/00365513.2022.2122076","DOIUrl":"https://doi.org/10.1080/00365513.2022.2122076","url":null,"abstract":"Haptoglobin-related protein (Hpr) is a plasma protein with high sequence similarity to haptoglobin (Hp). Like Hp, Hpr also binds hemoglobin (Hb) with high affinity, but it does not bind to the Hb-Hp receptor CD163 on macrophages. The Hpr concentration is markedly lower than Hp in plasma and its regulation is not understood. In the present study, we have developed non-crossreactive antibodies to Hpr to analyze the Hpr concentration in 112 plasma samples from anonymized individuals and compared it to Hp. The results show that plasma Hpr correlated with Hp concentrations (rho = 0.46, p = .0001). Hpr accounts for on average 0.35% of the Hp/Hpr pool but up to 29% at low Hp levels. Furthermore, the Hpr concentrations were significantly lower in individuals with the Hp2-2 phenotype compared to those with the Hp2-1 or Hp1-1 phenotypes. Experimental binding analysis did not provide evidence that Hpr associates with Hp and in this way is removed via CD163. In conclusion, the Hpr concentration correlates to Hp concentrations and Hp-phenotypes by yet unknown mechanisms independent of CD163-mediated removal of Hb-Hp complexes.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"461-466"},"PeriodicalIF":2.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40373982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood urea nitrogen/albumin ratio on admission predicts mortality in patients with non ST segment elevation myocardial infarction. 入院时血尿素氮/白蛋白比可预测非ST段抬高型心肌梗死患者的死亡率。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-10-01 Epub Date: 2022-09-20 DOI: 10.1080/00365513.2022.2122075

Sarper Sevdımbas, Salim Satar, Muge Gulen, Selen Acehan, Armagan Acele, Gonca Koksaldı Sahin, Deniz Aka Satar

{"title":"Blood urea nitrogen/albumin ratio on admission predicts mortality in patients with non ST segment elevation myocardial infarction.","authors":"Sarper Sevdımbas, Salim Satar, Muge Gulen, Selen Acehan, Armagan Acele, Gonca Koksaldı Sahin, Deniz Aka Satar","doi":"10.1080/00365513.2022.2122075","DOIUrl":"https://doi.org/10.1080/00365513.2022.2122075","url":null,"abstract":"The aim of this study is to reveal the predictive power of biomarkers and SYNTAX (SX) score for short-term mortality in patients diagnosed with non-ST-segment elevation myocardial infarction (NSTEMI) in the emergency department. This is prospective observational cohort study. Demographic characteristics of the patients, laboratory parameters on admission, left ventricular ejection fraction (LVEF) percentages, affected vessels in angiography (CAG) and the treatment strategy [medical therapy, percutaneous transluminal coronary angioplasty (PTCA), coronary angio by-pass graft] and SX scores were recorded on the data collection form. ROC curve was used to investigate the predictivity of blood urea nitrogen/albumin ratio (BAR), procalcitonin, C-reactive protein (CRP), high sensitivity cardiac troponin I (Hs-cTnI), CRP to serum albumin ratio (CAR), neutrophil to lymphocyte ratio (NLR) and SX scores in mortality. Multivariate analysis of biomarkers and SX score was performed to estimate the patients' 30-day mortality. Of the 415 patients were included in the study. ROC analysis of BAR, CAR, CRP, Procalcitonin, Hs-cTnI, NLR and SX score to predict mortality was statistically significant. BAR (OR: 1.280, 95% CI: 1.113-1.472, p = .001) and SX score (OR: 1.071, 95% CI: 1.018-1.126, p = .007) were found to be independent predictors of 30 days mortality. LVEF reduction, SX score, the number of affected vessels and the frequency of LMCA lesions increase were found to be statistically significant in patients with BAR ≥4.8. BAR, which can be calculated easily and quickly on admission to the emergency department and in clinical practice, may be used to predict mortality in patients with NSTEMI.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"454-460"},"PeriodicalIF":2.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40373066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparison of the automatised and the optimised carbon monoxide rebreathing methods. 自动化和优化一氧化碳再呼吸方法的比较。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-10-01 Epub Date: 2022-09-21 DOI: 10.1080/00365513.2022.2122078

Katja Kellenberger, Thomas Steiner, Jon Peter Wehrlin

{"title":"Comparison of the automatised and the optimised carbon monoxide rebreathing methods.","authors":"Katja Kellenberger, Thomas Steiner, Jon Peter Wehrlin","doi":"10.1080/00365513.2022.2122078","DOIUrl":"https://doi.org/10.1080/00365513.2022.2122078","url":null,"abstract":"Recently, a new automated carbon monoxide (CO) rebreathing method (aCO) to estimate haemoglobin mass (Hbmass) was introduced. The aCO method uses the same CO dilution principle as the widely used optimised CO rebreathing method (oCO). The two methods differ in terms of CO administration, body position, and rebreathing time. Whereas with aCO, CO is administered automatically by the system in a supine position of the subject, with oCO, CO is administered manually by an experienced operator with the subject sitting. Therefore, the aim of this study was to quantify possible differences in Hbmass estimated with the two methods. Hbmass was estimated in 18 subjects (9 females, 9 males) with oCO using capillary blood samples (oCOc) and aCO taking simultaneously venous blood samples (aCOv) and capillary blood samples (aCOc). Overall, Hbmass was different between the three measurement procedures (F = 57.55, p < .001). Hbmass was lower (p < .001) for oCOc (737 g ± 179 g) than for both aCOv (825 g ± 189 g, -9.3%) and aCOc (835 g ± 189 g, -10.6%). There was no difference in Hbmass estimated with aCOv and aCOc procedures (p = .12). Three factors can likely explain the 10% difference in Hbmass: differences in calculations (including a factor for myoglobin flux), body position (distribution of CO in blood circulation) during rebreathing, and time of blood sampling. Moreover, the determination of Hbmass with aCO is possible with capillary blood sampling instead of venous blood sampling.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"474-480"},"PeriodicalIF":2.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40374864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Interference of preservatives on urinary iodine measurement by Sandell-Kolthoff method. 防腐剂对Sandell-Kolthoff法测定尿碘的干扰。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-10-01 Epub Date: 2022-08-06 DOI: 10.1080/00365513.2022.2107566

Elif Kaya, Murat Usta, Ömer Emecen, Sembol Yıldırmak

{"title":"Interference of preservatives on urinary iodine measurement by Sandell-Kolthoff method.","authors":"Elif Kaya, Murat Usta, Ömer Emecen, Sembol Yıldırmak","doi":"10.1080/00365513.2022.2107566","DOIUrl":"https://doi.org/10.1080/00365513.2022.2107566","url":null,"abstract":"This report aimed to determine the effects of different preservatives used to maintain the integrity of urine samples for a 24-hour urine collection on urinary iodine measurement by the Sandell-Kolthoff method in vitro. The selected preservatives were hydrochloric acid, acetic acid, boric acid, sodium carbonate, sodium fluoride, thymol with isopropanol, sodium chloride with formalin, and Saccomanno fixative. The test samples to which these preservatives were added and the control samples for basal measurements were prepared from the urine pool obtained from urine samples taken from healthy volunteers. The urinary iodine measurements were performed following the calculated minimum number of replicates in these prepared samples. The data analysis of the interferences of the preservatives was performed based on the Clinical and Laboratory Standards Institute EP7-A2 guideline. The preservatives that had more acceptable effects on the urinary iodine measurement compared to the others were sodium carbonate (0.5% w/v) and thymol (10% w/v) with isopropanol (0.25% v/v) for 24-hour storage at room temperature. This report presented reliable and usable data revealing the effects of preservatives, which are frequently used to maintain the integrity of urine samples for a 24-hour urine collection, on urinary iodine measurement. There are need to reveal the possible effects of potential exogenous interfering substances and the pathological levels of endogenous analytes or shaped elements in the urine matrix on methods with routine clinical use in making medical decisions.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"498-503"},"PeriodicalIF":2.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference intervals for platelet large cell ratio, platelet distribution width, plateletcrit and standard haematological parameters determined on the Sysmex XN-10 in a cohort of 30,917 Danish blood donors. 在30,917名丹麦献血者中，用Sysmex XN-10测定血小板大细胞比、血小板分布宽度、血小板电化学和标准血液学参数的参考区间。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-10-01 Epub Date: 2022-09-16 DOI: 10.1080/00365513.2022.2116727

Birgitte Sandfeld-Paulsen, Louise Ørnskov Pedersen, Trine Kolding Damgaard, Helle Pilgaard Kristiansen

{"title":"Reference intervals for platelet large cell ratio, platelet distribution width, plateletcrit and standard haematological parameters determined on the Sysmex XN-10 in a cohort of 30,917 Danish blood donors.","authors":"Birgitte Sandfeld-Paulsen, Louise Ørnskov Pedersen, Trine Kolding Damgaard, Helle Pilgaard Kristiansen","doi":"10.1080/00365513.2022.2116727","DOIUrl":"https://doi.org/10.1080/00365513.2022.2116727","url":null,"abstract":"With the introduction of the Sysmex XN-10, new platelet indices reflecting platelet maturity can be obtained alongside with a full blood count. Therefore, the need for verified reference intervals is present. The purpose of this study was to establish reference intervals for the platelet indices: platelet large cell ratio (P-LCR), platelet distribution width (PDW) and plateletcrit (PCT) in a large Scandinavian cohort. Furthermore, we aimed to verify previously established reference intervals of haematological parameters included in a full blood count. Blood samples were obtained from healthy Danish blood donors and analysed by use of the Sysmex XN-10 analyser (Sysmex, Kobe, Japan). Non-parametric 95% reference intervals were determined as the 2.5 and 97.5 percentile and presented with 90% confidence intervals (CI). In total, 30,917 blood donors were included and the following reference intervals were established: P-LCR, ratio: 17.3 (90% CI: 17.2 - 17.5) - 46.2 (90% CI:45.9 - 46.4); P DW, fL:9.5 (90% CI: 9.5 - 9.5) - 17.2 (90% CI: 17.2 - 17.3) and PCT, fraction: 0.18 (0.18 - 0.18) - 0.38 (0.38 - 0.39). The reference intervals were stable across age and sex. Furthermore, reference intervals for the remaining haematological parameters included in a full blood count were verified and found in line with the previously established reference intervals.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"439-445"},"PeriodicalIF":2.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40362132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experiences with point-of-care blood gas measurements in a prehospital setting. 院前现场血气测量的经验

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-10-01 Epub Date: 2022-09-16 DOI: 10.1080/00365513.2022.2122079

Jonas E Pedersen, Mads Nybo, Eva R B Petersen, Jimmy H Holm, Søren Mikkelsen, Stine T Zwisler

{"title":"Experiences with point-of-care blood gas measurements in a prehospital setting.","authors":"Jonas E Pedersen, Mads Nybo, Eva R B Petersen, Jimmy H Holm, Søren Mikkelsen, Stine T Zwisler","doi":"10.1080/00365513.2022.2122079","DOIUrl":"https://doi.org/10.1080/00365513.2022.2122079","url":null,"abstract":"In a prehospital setting, the use of various Point-of-Care tests (POCT) is extremely important in the handling of critically ill patients: Prehospital use of high-sensitivity cardiac troponins (hs-cTn) has become an integrated part of acute myocardial infarction diagnosis along with echocardiography [1]. Prehospital use of ultrasound has been introduced in emergency care settings [2], and its use is presumed to increase rapidly over time. Also, several studies have suggested use of plasma lactate to improve triage of high-risk trauma patients to predict the need of intensive care [3–5]. Blood gas analyses are used in acute traumatised or ill patients to assess the degree of respiratory insufficiency, and in disorders of acid-base balance as well as in monitoring patients subjected to mechanical ventilation. During the recent years, blood gas analyses including electrolytes and lactate has become part of the prehospital analyses repertoire [6]. They have the potential to increase diagnostic ability and to accelerate and guide diagnosis and treatment at the prehospital site. We have previously described implementation of a blood gas analyser, the ABL90 FLEX (Radiometer, Denmark), in a ground-based anaesthesiologist-staffed Mobile Emergency Care Unit (MECU) [7]. Furthermore, we have investigated whether access to arterial blood gas analysis increased the prehospital diagnostic accuracy by prehospital anaesthesiologists [8] and showed that it significantly increased the quality of treatment provided to acute critically ill patients. The aim of the present paper is in a more casuistic way to describe advantages of rapid access to full blood gas analyses in a prehospital setting with two cases exemplifying its usefulness. Further, it is important to discuss advantages and limitations in the use of a POCT blood gas analyser.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"495-497"},"PeriodicalIF":2.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40363405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between oxidative stress and autophagy and apoptosis in patients with paroxysmal atrial fibrillation. 阵发性心房颤动患者氧化应激与自噬和细胞凋亡的关系。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2022-09-01 Epub Date: 2022-07-19 DOI: 10.1080/00365513.2022.2100274

Halil Fedai, Ibrahim Halil Altiparmak, Mustafa Begenc Tascanov, Zulkif Tanriverdi, Asuman Bicer, Fatih Gungoren, Recep Demirbag, Ismail Koyuncu

{"title":"The relationship between oxidative stress and autophagy and apoptosis in patients with paroxysmal atrial fibrillation.","authors":"Halil Fedai, Ibrahim Halil Altiparmak, Mustafa Begenc Tascanov, Zulkif Tanriverdi, Asuman Bicer, Fatih Gungoren, Recep Demirbag, Ismail Koyuncu","doi":"10.1080/00365513.2022.2100274","DOIUrl":"https://doi.org/10.1080/00365513.2022.2100274","url":null,"abstract":"Although the pathophysiology of paroxysmal atrial fibrillation (PAF) is not fully known, oxidative stress (OS) and atrial remodeling seem to be important triggers. Autophagy and apoptosis which are the types of cell death are fundamental processes in the human body. Although they investigated in many diseases, no study evaluated these parameters in PAF patients. We aimed to investigate autophagy and apoptosis which may be associated with atrial remodeling, and to show whether these factors are associated with OS in PAF patients. In this study, 44 PAF patients admitted to our clinic and 44 healthy volunteers were included. Serum total oxidative stress (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and ATG5 for autophagy and serum M30 for apoptosis were studied. Serum TOS, OSI, ATG5, M30 and left atrium (LA) diameter were higher, while TAS was lower in PAF group than the control group (p < 0.001, for all). ATG5 was positively correlated with TOS, OSI and LA, whereas negatively correlated with TAS. Also, M30 was positively correlated with TOS and OSI, whereas negatively correlated with TAS. Logistic regression analysis showed that TOS (P = 0.002), ATG5 (p = 0.013) and M30 (p = 0.006) were independent predictors of the PAF. It also found that ATG5 was the only independent predictor of LA enlargement in linear regression analysis. Our study showed that ATG5 and M30 were increased, and they were correlated with OS in patients with PAF. Therefore, we suggest that autophagy and apoptosis may play an important role in the PAF process.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"391-397"},"PeriodicalIF":2.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40616469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3