Scandinavian Journal of Clinical and Laboratory Investigation最新文献_第10页

Comparison of rocket and crossed immuno-electrophoresis assays for determination of the level of actin complexing of Gc globulin. 火箭和交叉免疫电泳法测定Gc球蛋白肌动蛋白络合水平的比较。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701326909

C S Jørgensen, F V Schiødt, B Dahl, I Laursen, G Houen

{"title":"Comparison of rocket and crossed immuno-electrophoresis assays for determination of the level of actin complexing of Gc globulin.","authors":"C S Jørgensen, F V Schiødt, B Dahl, I Laursen, G Houen","doi":"10.1080/00365510701326909","DOIUrl":"https://doi.org/10.1080/00365510701326909","url":null,"abstract":"Objective: Gc globulin (vitamin D-binding protein) is a component of the extracellular actin scavenger system. The level of Gc globulin is reduced in patients with fulminant hepatic failure, septic shock and trauma. Furthermore, low levels of Gc globulin in patients with fulminant hepatic failure and multiple trauma have been found to correlate with the morbidity and mortality of patients. Owing to a large increase in the turnover of Gc globulin upon complex formation with actin, it may be important to determine both the total Gc globulin concentration and the degree of complexing with actin for estimating the clinical prognosis of a patient. For this reason, we have compared a crossed immuno-electrophoresis method (CIE), suitable for visualizing the degree of complexing with actin, with a rocket immuno-electrophoresis method (RIE), previously used for determination of the complex degree.Material and methods: Sera from healthy donors and from patients with acetaminophen-induced liver disease or trauma were investigated using CIE, RIE and enzyme-linked immunosorbent assay (ELISA).Results: Using the CIE, no Gc globulin-actin complexes were detected among healthy donors. Complexes were present in 21 of 39 patients with liver disease and 3 of 37 trauma patients. High complex ratios (> 20 %) were found in 6 of 7 patients with hepatic encephalopathy. Using the RIE, complexes were detected in most samples.Conclusion: The results show that the CIE method may be used for determining the degree of actin complexing in conjunction with ELISA or RIE in determining the levels of total Gc globulin.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"767-77"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701326909","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40961235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Experimental hyperhomocysteinemia disturbs bone metabolism in rats. 实验性高同型半胱氨酸血症扰乱大鼠骨代谢。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701342088

S Ozdem, S Samanci, A Tasatargil, A Yildiz, G Sadan, L Donmez, M Herrmann

{"title":"Experimental hyperhomocysteinemia disturbs bone metabolism in rats.","authors":"S Ozdem, S Samanci, A Tasatargil, A Yildiz, G Sadan, L Donmez, M Herrmann","doi":"10.1080/00365510701342088","DOIUrl":"https://doi.org/10.1080/00365510701342088","url":null,"abstract":"Objective: To investigate whether experimental hyperhomocysteinemia (HHCY) can induce adverse changes in bone metabolism.Methods: Blood and urine samples were collected from rats fed with a methionine-enriched diet (HHCY, n = 18) or an isocaloric control diet (control, n = 10) for 12 weeks. Biochemical bone turnover markers (osteocalcin, hydroxyproline, N-terminal collagen I telopeptides and homocysteine (HCY), folate and vitamin B12) were measured. Whole body bone mineral density (BMD) was assessed by dual energy X-ray absorptiometry.Results: HCY was significantly higher in HHCY than in control rats (16.2 versus 3.2 micromol/L; p = 0.0006). Bone resorption parameters hydroxyproline (1.60 +/- 0.6 versus 0.85 +/- 0.4; p<0.05) and N-terminal collagen I telopeptides (150.8 +/- 78 versus 48.1 +/- 26 nmol/L BCE; p<0.05) increased, whereas bone formation marker osteocalcin (9.01 +/- 3.8 versus 15.07 +/- 4.2 ng/mL; p<0.05) decreased in HHCY compared to control rats. The relation N-terminal collagen I telopeptides/osteocalcin significantly increased in HHCY compared to control rats (13.14 +/- 3.1 versus 4.14 +/- 1.9). BMD measurement did not reveal any differences between groups.Conclusion: These findings demonstrate a significant modification of bone turnover in HHCY rats. The relation between bone resorption and formation indicates a shift toward bone resorption, which might be a plausible explanation for the relation between HHCY and fracture risk.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"748-56"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701342088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40961237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Performance of automated measurement of antibodies to cyclic citrullinated peptide in the routine clinical laboratory. 常规临床实验室环瓜氨酸肽抗体自动测定的性能。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701408582

C M Van Campenhout, K A Van Cotthem, W J Stevens, L S De Clerck

{"title":"Performance of automated measurement of antibodies to cyclic citrullinated peptide in the routine clinical laboratory.","authors":"C M Van Campenhout, K A Van Cotthem, W J Stevens, L S De Clerck","doi":"10.1080/00365510701408582","DOIUrl":"https://doi.org/10.1080/00365510701408582","url":null,"abstract":"Objective: To evaluate the performing technical and clinical characteristics of an automated system for routine measurement of anticyclic citrullinated peptide antibodies (aCCP), a new marker for rheumatoid arthritis (RA).Material and methods: Reproducibility, repeatability and linearity of aCCP, as measured by an automated fluorescent enzyme immunoassay (FEIA/Phadia), were evaluated and compared with the performance of a manual ELISA technique (Axis Shield Diagnostics). Clinical verification of both methods included estimation of sensitivity in RA patients (n = 42) and specificity in well-characterized non-RA autoimmune disease controls (n = 49) and healthy subjects (n = 39).Results: Precision studies showed a coefficient of variation between 4.9 % and 10 % for the FEIA technique and between 6.35% and 19% for the ELISA technique. Both systems showed good linear response. Sensitivity of aCCP for RA was 74% for FEIA and 79% for ELISA. Specificity was 100% for both methods, as calculated for healthy subjects. For non-RA-diseased controls, specificities of 98% and 94% were obtained for FEIA and ELISA, respectively. Both methods were concordant in 97% of cases. Increasing the cut-off for the ELISA system from >5 U/mL to >11 U/mL resulted in lower sensitivity (71.4%) but higher specificity (98.0%), i.e. improved discriminating power between RA and non-RA and 100% agreement between both methods.Conclusion: Automated FEIA measurement of aCCP in the routine clinical laboratory improves imprecision compared to the manual ELISA. Our preliminary results suggest that an increase in cut-off for the ELISA can improve specificity to RA from 94% to 98 %.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"859-67"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701408582","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40960542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Biological variation and reference intervals for circulating osteopontin, osteoprotegerin, total soluble receptor activator of nuclear factor kappa B ligand and high-sensitivity C-reactive protein. 循环骨桥蛋白、骨保护素、核因子κ B配体总可溶性受体激活剂和高敏c反应蛋白的生物学变异和参考区间。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701432509

H P Sennels, S Jacobsen, T Jensen, M S Hansen, M Ostergaard, H J Nielsen, S Sørensen

{"title":"Biological variation and reference intervals for circulating osteopontin, osteoprotegerin, total soluble receptor activator of nuclear factor kappa B ligand and high-sensitivity C-reactive protein.","authors":"H P Sennels, S Jacobsen, T Jensen, M S Hansen, M Ostergaard, H J Nielsen, S Sørensen","doi":"10.1080/00365510701432509","DOIUrl":"https://doi.org/10.1080/00365510701432509","url":null,"abstract":"Objective: Monitoring inflammatory diseases and osteoclastogenesis with osteopontin (OPN), osteoprotegerin (OPG), total soluble receptor activator of nuclear factor kappa B ligand (total sRANKL) and high-sensitivity C-reactive protein (hsCRP) has recently attracted increased interest. The purpose of our study was to determine reference intervals, variability caused by sampling time, biological variation and stability after repeated freeze-thaw cycles of circulating levels of OPN, OPG, total sRANKL and hsCRP.Material and methods: Plasma OPN and plasma OPG concentrations were determined with sandwich ELISA; serum total sRANKL concentration was determined using a two-site sandwich ELISA; and hsCRP was analysed by turbidimetry in 300 Danish blood donors (183 M and 117 F) with a median age of 43 years (range 18-64 years). Variability due to biological variation and sampling time was studied in serial samples from 38 healthy subjects.Results: The 95th percentiles in the donors were 76 microg/L for OPN, 4.2 pmol/L for OPG, 40.2 nmol/L for total sRANKL and 12 mg/L for hsCRP. The overall medians for both genders were 51 microg/L, 2.2 pmol/L, 0.66 nmol/L and 1.0 mg/L, respectively. We found a significant correlation between hsCRP and OPN (rho = 0.173; p<0.003). The biological within-subject variations were calculated to be 8.2 % for OPN, 8.8% for total sRANKL and 50% for hsCRP.Conclusions: Reference intervals have been established with a high analytic performance for OPN and an acceptable analytic performance for OPG and total sRANKL. The study revealed low biological variation for OPN and total sRANKL and high biological variation for hsCRP.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"821-35"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701432509","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40960544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Faecal and serum levels of eosinophil cationic protein in a healthy paediatric population. 健康儿童人群粪便和血清嗜酸性阳离子蛋白水平

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701308337

A C Silva, L Levy, J C Trindade, P Mendonça, C Silva, A I Lopes

{"title":"Faecal and serum levels of eosinophil cationic protein in a healthy paediatric population.","authors":"A C Silva, L Levy, J C Trindade, P Mendonça, C Silva, A I Lopes","doi":"10.1080/00365510701308337","DOIUrl":"https://doi.org/10.1080/00365510701308337","url":null,"abstract":"Background: Eosinophil cationic protein (ECP) has been regarded as an excellent marker of eosinophil activation in various diseases where eosinophil-mediated inflammation plays a role. Recently, it has been suggested as a faecal marker of intestinal inflammation in several immune-mediated diseases with gastrointestinal expression. Owing to the scarcity of information at paediatric age, the establishment of reference values is necessary before further clinical studies.Objective: To determine faecal and serum ECP levels in healthy children and their association with other biological parameters, thereby providing background additional validation data for this age group.Methods: Faecal and serum ECP levels were available from healthy Caucasian children recruited at a regular outpatient clinic. Exclusion criteria were: chronic illnesses, acute illness, mucosal bleeding and recent pharmacological medication. Faecal and serum ECP levels and faecal a1AT were determined by commercial radioimmunoassay and serum IgE by fluoroenzyme immunoassay Uni-CAP.Results: Mean and median faecal ECP levels were 1.93 microg/g and 1.20 microg/g, respectively (range 0.41-22.20),while the corresponding serum ECP levels were 13.50 microg/L and 9.54 microg/L, respectively (range 0.20-74.8). The cut-offs found were 2.80 microg/g and 16.89 microg/L for faecal and serum ECP, respectively. A significant (p=0.001) increase in serum, but not in faecal, ECP levels was found among patients with high peripheral eosinophil blood count. Neither faecal nor serum ECP levels were influenced by serum IgE levels.Conclusions: Faecal and serum ECP levels, as determined in the present study, add background information concerning reference levels at paediatric age for further studies indifferent clinical settings.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"757-66"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701308337","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40961236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Autoantibodies to the proteasome in monosymptomatic optic neuritis may predict progression to multiple sclerosis. 单症状性视神经炎的蛋白酶体自身抗体可预测多发性硬化症的进展。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701342062

N H Beyer, J Milthers, A-M Bonde Lauridsen, G Houen, J Lautrup Frederiksen

{"title":"Autoantibodies to the proteasome in monosymptomatic optic neuritis may predict progression to multiple sclerosis.","authors":"N H Beyer, J Milthers, A-M Bonde Lauridsen, G Houen, J Lautrup Frederiksen","doi":"10.1080/00365510701342062","DOIUrl":"https://doi.org/10.1080/00365510701342062","url":null,"abstract":"Objective: Proteasome autoantibodies (PAB) have been found in multiple sclerosis (MS) patient sera and cerebrospinal fluid (CSF). Presence of PAB could thus be a possible diagnostic marker for MS. We investigated whether PAB serum status in acute monosymptomatic optic neuritis (ON) and MS differed significantly from that of healthy controls, and whether or not PAB status is predictive of later MS development in patients with ON.Material and methods: Sera from ON patients, MS patients and healthy donors were analysed retrospectively using ELISA. Subsequently, a small group of PAB-positive samples were subjected to SDS-PAGE, immunoblotting and 2-D PAGE.Results: We found that 20 % (6/30) ON patients, 47 % (22/47) MS patients and 9 % (7/81) controls tested PAB positive using ELISA analysis. High PAB levels were found in 2 (4 %) MS patients, 1 (3 %) ON patient and 2 (3 %) controls. PAB positivity in ELISA was confirmed by immunoblotting. Separation of proteasome subunits by 2D PAGE followed by immunoblotting revealed no particular PAB subunit preference.Conclusions: A retrospective search in available patient files revealed that 6 of 6 (100.0 %) PAB-positive ON patients developed MS over time. Eight of 24 (33 %) PAB-negative ON patients developed MS over time and 47 % (14/30) of all ON patients developed MS. A series of patient CSF was analysed by ELISA to assess the possible correlation between PAB status of concurrent serum and CSF samples, but no correlation was found. However, the results from the six PAB-positive ON patients could potentially be of prognostic value.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"696-706"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701342062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40960801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Effect of freeze-thaw cycles on serum measurements of AFP, CEA, CA125 and CA19-9. 冻融循环对血清AFP、CEA、CA125和CA19-9测定的影响。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701297480

Y-C Gao, Z-B Yuan, Y-D Yang, H-K Lu

{"title":"Effect of freeze-thaw cycles on serum measurements of AFP, CEA, CA125 and CA19-9.","authors":"Y-C Gao, Z-B Yuan, Y-D Yang, H-K Lu","doi":"10.1080/00365510701297480","DOIUrl":"https://doi.org/10.1080/00365510701297480","url":null,"abstract":"AFP, CEA, CA125 and CA19-9 are commonly used serum tumour markers (TMs) in clinical practice, although their quantification by immunoassay may be influenced by pre-analytical sample handling. Though the effect of repetitive freeze-thaw cycles is generally recognized, it is not clear in detail. The present study measured (CLIA) these TMs in serum samples freshly separated and after each of five freeze-thaw cycles, in which the samples were frozen at -40 degrees C for 10 months at cycle 4 and 2 h at other cycles. Statistical analysis with the General Linear Model for Repeated Measures revealed significant decreases in the measurements of the four TMs, with the least decrease of 6.8 % for CA125 and the most decrease of 18.2 % for CA19-9 after the last cycle, and an overwhelming single cycle decrease of mean 7.7 % at cycle 4 for AFP, CEA and CA125, of 7.5 % and 9.3 % at cycles 4 and 5 for CA19-9. So it seems that measurements of AFP, CEA and CA125 are more readily affected by long-term frozen storage compared with frequent freezing-thawing, while CA19-9 is relatively unstable under both conditions.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"741-7"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701297480","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40961240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Cardiovascular disease and type 2 diabetes mellitus: a multifaceted symbiosis. 心血管疾病和2型糖尿病:一个多方面的共生关系。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701408558

O E Johansen

{"title":"Cardiovascular disease and type 2 diabetes mellitus: a multifaceted symbiosis.","authors":"O E Johansen","doi":"10.1080/00365510701408558","DOIUrl":"https://doi.org/10.1080/00365510701408558","url":null,"abstract":"Chronic hyperglycaemia (e.g. type 2 diabetes mellitus (T2DM) and prediabetes) in humans is associated with an increased risk of cardiovascular (CV) complications, and, vice versa, the presence of CV complications (e.g. myocardial infarction, stroke or intermittent claudication) among patients heightens the risk of T2DM or prediabetes. In both cases (i.e. chronic hyperglycaemia and CV complications), significant diagnostic and treatment challenges resulting from a broad range of factors may serve as barriers to reducing the deleterious societal impact of T2DM and prediabetes. These challenges often include clinicians: failing to intervene early and aggressively enough among patients with T2DM to achieve CV risk factor control; failing to efficaciously identify T2DM patients with already established CV complications; and failing proactively to assess individuals at high risk for T2DM. This review discusses the apparent symbiosis between CV disease and T2DM, with a focus on identifying patients with established T2DM or at risk for T2DM; traditional and novel risk factors and markers for CV disease in T2DM; challenges related to diagnosing CV disease in T2DM; and organization of T2DM care in order to prevent CV complications. These are issues that require attention because identifying patients at high risk for T2DM can halt or reduce their further glycaemic deterioration if addressed properly, and because novel markers and non-invasive tests could be applied in patients with T2DM as a means of detecting and possibly treating unrecognized CV disease in time. Furthermore, several approaches for T2DM care can be effective in controlling the CV risk factors contributing to CV complications.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"786-800"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701408558","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40960802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

External quality assessment in the measurement of haemoglobin by blood gas analysers in Belgium. 比利时血气分析仪测量血红蛋白的外部质量评价。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701297464

M Van Blerk, W Coucke, B Chatelain, W Goossens, K Jochmans, K Meeus, K Mertens, O Pradier, J-L Rummens, J-M Scheiff, J-C Libeer

{"title":"External quality assessment in the measurement of haemoglobin by blood gas analysers in Belgium.","authors":"M Van Blerk, W Coucke, B Chatelain, W Goossens, K Jochmans, K Meeus, K Mertens, O Pradier, J-L Rummens, J-M Scheiff, J-C Libeer","doi":"10.1080/00365510701297464","DOIUrl":"https://doi.org/10.1080/00365510701297464","url":null,"abstract":"Objective: The Belgian national External Quality Assessment Scheme (EQAS) for haematology organized a survey to assess the reliability of haemoglobin (Hb) measurements with the blood gas analysers (BGAs) currently available in Belgian hospitals.Material and methods: All hospital laboratories received two specimens of fresh EDTA anticoagulated whole blood and were asked to determine the Hb concentration using both the conventional haematology analyser (HA) and all BGAs in the hospital. Ninety-seven hospital laboratories participated in the study and a total of 166 results were reported. The BGAs used (grouped according to technology) were Rapidlab 845, 855, 865 (Bayer 1, n = 41), Rapidlab 1245, 1265, Rapidpoint 405 (Bayer 2, n = 19), GEM Premier 3000 (Instrumentation Laboratory, IL, n = 13), ABL 500 and 600 series (Radiometer 1, n = 13), ABL 700 and 800 series (Radiometer 2, n = 35), Omni C, S5 (Roche 1, n = 7), Omni 3, 6, 9, S2, S4, S6 (Roche 2, n = 21).Results: For the BGAs from Bayer, Radiometer and Roche, interlaboratory variation ranged from 0.6 % to 4.1 %, indicating good precision and close agreement between centres. A significant negative bias observed on the GEM Premier 3000 using the EDTA anticoagulated blood samples did not appear to be present in fresh heparinized whole blood samples. There was no significant difference in imprecision and bias between Hb measurements on BGA situated in and outside the central laboratory.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"735-40"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701297464","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40961239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Patient-centred care--preanalytical factors demand attention: a questionnaire study of venous blood sampling and specimen handling. 以患者为中心的护理-分析前因素需要注意:静脉血采样和标本处理的问卷研究。

IF 2.1

Scandinavian Journal of Clinical and Laboratory Investigation Pub Date : 2007-01-01 DOI: 10.1080/00365510701370675

O Wallin, J Söderberg, B Van Guelpen, C Brulin, K Grankvist

{"title":"Patient-centred care--preanalytical factors demand attention: a questionnaire study of venous blood sampling and specimen handling.","authors":"O Wallin, J Söderberg, B Van Guelpen, C Brulin, K Grankvist","doi":"10.1080/00365510701370675","DOIUrl":"https://doi.org/10.1080/00365510701370675","url":null,"abstract":"Objective: Most mistakes in laboratory medicine are the result of human error occurring before the blood sample reaches the laboratory. This survey of preanalytical procedures was designed to identify sources of error and potential targets for quality improvement strategies.Material and methods: The staff in a highly specialized surgical ward at a university hospital completed a questionnaire addressing the collection and handling of venous blood samples in plastic vacuum test-tubes for general clinical chemistry testing.Results: The results suggest that venous blood sampling instructions are not always followed. When uncertain about how a sample should be collected, the majority of respondents rely on potentially poor sources of information, such as out-of-date printed instructions or the advice of a colleague, rather than consult up-to-date electronic instructions. Furthermore, they do not always report errors and the referrals are not always handled according to sampling instructions. The respondents were highly motivated, however, and had a strong interest in receiving further education in, and assuming increased responsibility for, venous blood sampling procedures in the ward.Conclusions: We believe that the introduction of standardized routines and regular staff training, combined with an exchange of the existing paper-based referral management system with an electronic system for managing referrals, could increase safety in the preanalytical process, with positive effects on patient safety. Given the importance of venous blood samples in patient care, a more extensive study covering other hospital wards and primary health-care centres is needed.","PeriodicalId":501634,"journal":{"name":"Scandinavian Journal of Clinical and Laboratory Investigation","volume":" ","pages":"836-47"},"PeriodicalIF":2.1,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00365510701370675","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40960454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24