bioRxiv - Bioengineering最新文献_第3页

Rapid production of modified cattle and sheep from spermatid-like stem cells 利用类精子干细胞快速生产改良牛羊

bioRxiv - Bioengineering Pub Date : 2024-09-14 DOI: 10.1101/2024.09.10.612264

Lei Yang, Anqi Di, Lishuang Song, Xuefei Liu, Di Wu, Song Wang, Zhengting Hao, Lige Bu, Chunling Bai, Guanghua Su, Zhuying Wei, Li Zhang, Zhonghua Liu, Shaorong Gao, Guangpeng Li

引用次数: 0

Proposing a methodology for axon-centric analysis of IOP-induced mechanical insult 提出一种以轴突为中心分析眼压引起的机械损伤的方法

bioRxiv - Bioengineering Pub Date : 2024-09-14 DOI: 10.1101/2024.09.09.611631

Manik Bansal, Bingrui Wang, Susannah Waxman, Fugiang Zhong, Yi Hua, Yuankai Lu, Juan Reynaud, Brad Fortune, Ian A Sigal

{"title":"Proposing a methodology for axon-centric analysis of IOP-induced mechanical insult","authors":"Manik Bansal, Bingrui Wang, Susannah Waxman, Fugiang Zhong, Yi Hua, Yuankai Lu, Juan Reynaud, Brad Fortune, Ian A Sigal","doi":"10.1101/2024.09.09.611631","DOIUrl":"https://doi.org/10.1101/2024.09.09.611631","url":null,"abstract":"Purpose. IOP-induced mechanical insult on retinal ganglion cell axons within the optic nerve head (ONH) is believed to be a key factor in axonal damage and glaucoma. However, most studies focus on tissue-level mechanical deformations, overlooking that axons are long and thin, and that their susceptibility to damage likely depends on the insult's type (e.g. stretch/compression) and orientation (longitudinal/transverse). We propose an axon-centric approach to quantify IOP-induced mechanical insult from an axon perspective. Methods. We used optical coherence tomography (OCT) scans from a healthy monkey eye along with histological images of cryosections to reconstruct the axon-occupied volume including detailed lamina cribrosa (LC) pores. Tissue-level strains were determined experimentally using digital volume correlation from OCT scans at baseline and elevated IOPs, then transformed into axonal strains using axon paths estimated by a fluid mechanics simulation. Results. Axons in the LC and post-LC regions predominantly experienced longitudinal compression and transverse stretch, whereas those in the pre-LC and ONH rim mainly suffered longitudinal stretch and transverse compression. No clear patterns were observed for tissue-level strains. Conclusions. Our approach allowed discerning axonal longitudinal and transverse mechanical insults, which are likely associated with different mechanisms of axonal damage. The technique also enabled quantifying insult along individual axon paths, providing a novel link relating the retinal nerve fiber layer and the optic nerve through the LC via individual axons. This is a promising approach to establish a clearer connection between IOP-induced insult and glaucoma. Further studies should evaluate a larger cohort.","PeriodicalId":501308,"journal":{"name":"bioRxiv - Bioengineering","volume":"202 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic Virus-Like Particles to control cell functions 控制细胞功能的仿生病毒颗粒

bioRxiv - Bioengineering Pub Date : 2024-09-14 DOI: 10.1101/2024.09.14.612851

Hasna Maayouf, Thomas Dos Santos, Alphonse Boche, Rayane Hedna, Kaspars Tars, Isabelle Brigaud, Tatiana Petithory, Franck Carreiras, Carole Arnold, Ambroise Lambert, Laurent Pieuchot

{"title":"Biomimetic Virus-Like Particles to control cell functions","authors":"Hasna Maayouf, Thomas Dos Santos, Alphonse Boche, Rayane Hedna, Kaspars Tars, Isabelle Brigaud, Tatiana Petithory, Franck Carreiras, Carole Arnold, Ambroise Lambert, Laurent Pieuchot","doi":"10.1101/2024.09.14.612851","DOIUrl":"https://doi.org/10.1101/2024.09.14.612851","url":null,"abstract":"Biomimetic cues from the extracellular matrix (ECM) are essential for optimizing cell microenvironments and biomaterials. While native ECM proteins or synthetic peptides offer potential solutions, challenges such as production cost, solubility, and conformational stability limit their use. Here, we present the development of virus-like particles (VLPs) derived from the AP205 RNA phage displaying peptides from key ECM proteins and evaluate their biological activity in a variety of assays. We show that our engineered VLPs can effectively stimulate cell adhesion, migration, proliferation and differentiation. By comparing focal adhesions formed by RGD VLPs with their parent protein, fibronectin, we elucidate both similarities and differences in cell interactions. In addition, we construct heterodimeric particles co-expressing RGD with differentiation peptides and demonstrate retention of bioactivity in a multi-peptide context. This study establishes AP205 VLPs as versatile nanoscale platforms capable of tuning cell functions, with promising applications in nanomedicine and biomaterials.","PeriodicalId":501308,"journal":{"name":"bioRxiv - Bioengineering","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142255987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enantiomeric histidine-rich peptide coacervates enhance antigen delivery to T cells 富含组氨酸的对映体多肽共凝胶能增强向 T 细胞递送抗原的能力

bioRxiv - Bioengineering Pub Date : 2024-09-14 DOI: 10.1101/2024.09.10.612317

Ushasi Pramanik, Anirban Das, Elise M. Brown, Heather L. Struckman, Huihao Wang, Samuel Stealey, Macy L. Sprunger, Wasim Abdul, Jonathan Fascetti, Jagannath Mondal, Jonathan R. Silva, Silviya P. Zustiak, Meredith E. Jackrel, Jai S. Rudra

{"title":"Enantiomeric histidine-rich peptide coacervates enhance antigen delivery to T cells","authors":"Ushasi Pramanik, Anirban Das, Elise M. Brown, Heather L. Struckman, Huihao Wang, Samuel Stealey, Macy L. Sprunger, Wasim Abdul, Jonathan Fascetti, Jagannath Mondal, Jonathan R. Silva, Silviya P. Zustiak, Meredith E. Jackrel, Jai S. Rudra","doi":"10.1101/2024.09.10.612317","DOIUrl":"https://doi.org/10.1101/2024.09.10.612317","url":null,"abstract":"Peptides and peptidomimetics that self-assemble via LLPS have recently emerged as building blocks for fabricating functional biomaterials due to their unique physicochemical properties and dynamic nature. One of life's most distinctive signatures is its selectivity for chiral molecules and, to date, coacervates comprised of D-amino acids have not been reported. Here, we demonstrate that histidine-rich repeats of (GHGXY)4 (X=L/V/P) and their enantiomers undergo LLPS opening new avenues for enhancing coacervate stability. Through a series of biophysical studies, we find that LLPS kinetics, droplet size, fusion, and encapsulation efficiency are dictated by the primary sequence. Further, these coacervates can encapsulate therapeutic cargo which are then internalized via endocytic mechanisms. Finally, we show that the coacervates enhance antigen presentation to CD4+ and CD8+ T cells resulting in robust proliferation and production of functional cytokines. Collectively, our study describes the development and characterization of enantiomeric peptide coacervates as attractive vaccine delivery vehicles with tunable physicochemical properties.","PeriodicalId":501308,"journal":{"name":"bioRxiv - Bioengineering","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Randomly oriented microgrooved hydrogel guides cellular motility, modulates speed, and governs directionality of cellular spread 随机定向微槽水凝胶引导细胞运动、调节速度并控制细胞扩散的方向性

bioRxiv - Bioengineering Pub Date : 2024-09-14 DOI: 10.1101/2024.09.10.612339

Biswajoy Ghosh, Krishna Agarwal

{"title":"Randomly oriented microgrooved hydrogel guides cellular motility, modulates speed, and governs directionality of cellular spread","authors":"Biswajoy Ghosh, Krishna Agarwal","doi":"10.1101/2024.09.10.612339","DOIUrl":"https://doi.org/10.1101/2024.09.10.612339","url":null,"abstract":"Cell migration is a fundamental biological process, yet the mechanisms underlying how cells sense and navigate complex environments remain poorly understood. In this study, we developed a system of randomly oriented microgrooves, designed at cellular length scales, to explore motility intelligence in response to varied topographies. These microgrooves allowed cells to freely choose their migratory paths, revealing key insights into how cells sense and adapt to topological cues. Using fibroblast cells migrating over these grooved substrates, we examined cellular processes such as actin cytoskeleton remodeling, cell adhesion dynamics, and the impact of groove alignment on migration speed and directionality. Our results demonstrate that cells align their cytoskeletal structures to groove geometries, forming actin-rich anchors that enhance migration in groove-aligned environments. Cells migrating in grooves aligned with their intrinsic polarity exhibited faster, more directed migration compared to those in misaligned or control conditions. This work advances our understanding of cell-topology interaction and provides new perspectives for tissue engineering applications in cancer therapy and wound healing.","PeriodicalId":501308,"journal":{"name":"bioRxiv - Bioengineering","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Causality Analysis of Protein Corona Composition: Phosphatidylcholine-Enhances Plasma Proteome Profiling by Proteomics 蛋白质电晕组成的因果关系分析：通过蛋白质组学分析磷脂酰胆碱增强血浆蛋白质组图谱

bioRxiv - Bioengineering Pub Date : 2024-09-14 DOI: 10.1101/2024.09.10.612356

Arshia Rafieioskouei, Kenneth Rogale, Amirata Saei Dibavar, Morteza Mahmoudi, Borzoo Bonakdarpour

{"title":"Causality Analysis of Protein Corona Composition: Phosphatidylcholine-Enhances Plasma Proteome Profiling by Proteomics","authors":"Arshia Rafieioskouei, Kenneth Rogale, Amirata Saei Dibavar, Morteza Mahmoudi, Borzoo Bonakdarpour","doi":"10.1101/2024.09.10.612356","DOIUrl":"https://doi.org/10.1101/2024.09.10.612356","url":null,"abstract":"The study of the protein corona, the immediate and evolving biomolecular coating that forms on the surface of nanoparticles when exposed to a biological environment, is a crucial area in nanomedicine. This phenomenon significantly influences the behavior, functionality, and biological interactions of nanoparticles with biosystems. Until now, conclusions regarding the role of the protein corona in specific biological applications have been based on establishing correlation rather than causation. By understanding causality, researchers can predict how changes in nanoparticle properties or biological conditions will affect protein corona composition, in turn affecting the nanoparticle interactions with the biosystems and their applications. This predictive capability is essential for designing nanoparticles with specific characteristics tailored for therapeutic and diagnostic nanomedicine applications. Here, we explore the concept of actual causality (by Halpern and Pearl) to mathematically prove how various small molecules, including metabolites, lipids, vitamins, and nutrients, spiked into plasma can induce diverse protein corona patterns on identical nanoparticles. This approach significantly enhances the depth of plasma proteome profiling. Our findings reveal that among the various spiked small molecules, phosphatidylcholine was the actual cause of the observed increase in the proteomic depth of the plasma sample. By considering the concept of causality in the field of protein corona, the nanomedicine community can substantially improve their ability to design safer and more efficient nanoparticles for both diagnostic and therapeutic purposes.","PeriodicalId":501308,"journal":{"name":"bioRxiv - Bioengineering","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunable picoliter-scale dropicle formation using amphiphilic microparticles with patterned hydrophilic patches 利用带有图案化亲水斑块的两亲性微颗粒形成可调节的皮升级液滴颗粒

bioRxiv - Bioengineering Pub Date : 2024-09-13 DOI: 10.1101/2024.09.09.611468

Xinpei Song, Shreya Udani, Mengxing Ouyang, Mehmet Akif Sahin, Dino Di Carlo, Ghulam Destgeer

{"title":"Tunable picoliter-scale dropicle formation using amphiphilic microparticles with patterned hydrophilic patches","authors":"Xinpei Song, Shreya Udani, Mengxing Ouyang, Mehmet Akif Sahin, Dino Di Carlo, Ghulam Destgeer","doi":"10.1101/2024.09.09.611468","DOIUrl":"https://doi.org/10.1101/2024.09.09.611468","url":null,"abstract":"Microparticle-templated droplets or dropicles have recently gained interest in the fields of diagnostic immunoassays, single-cell analysis, and digital molecular biology. Amphiphilic particles have been shown to spontaneously capture aqueous droplets within their cavities upon mixing with an immiscible oil phase, where each particle templates a single droplet. Here, we fabricated an amphiphilic microparticle with four discrete hydrophilic patches embedded at the inner corners of a square-shaped hydrophobic outer ring of the particle (4C particle). 3D computational fluid dynamics simulations predicted droplet formation dynamics and differing equilibrium conditions depending on the patterning configuration. Experiments recapitulated equilibrium conditions enabling tunable dropicle configurations with reproducible volumes down to ~200pL templated by the amphiphilic particles. The dropicle configurations depended predominantly on the size of the hydrophilic patches of the 4C particles. This validated modeling approach can inform the design of dropicles with varying volumes and numbers per particle, which can be harnessed in new amplified bioassays for greater sensitivity, dynamic range, and statistical confidence.","PeriodicalId":501308,"journal":{"name":"bioRxiv - Bioengineering","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glassy Surfactants Enable Ultra-High Concentration Biologic Therapeutics 玻璃表面活性剂实现超高浓度生物疗法

bioRxiv - Bioengineering Pub Date : 2024-09-13 DOI: 10.1101/2024.09.09.612104

Carolyn K Jons, Alexander N Prossnitz, Noah Eckman, Changxin Dong, Eric Appel

{"title":"Glassy Surfactants Enable Ultra-High Concentration Biologic Therapeutics","authors":"Carolyn K Jons, Alexander N Prossnitz, Noah Eckman, Changxin Dong, Eric Appel","doi":"10.1101/2024.09.09.612104","DOIUrl":"https://doi.org/10.1101/2024.09.09.612104","url":null,"abstract":"Protein therapeutics, like peptides and antibodies, have become critical to healthcare. Despite their exceptional potency and specificity, biopharmaceuticals are prone to aggregation, often necessitating low formulation concentrations as well as cold storage and distribution to maintain stability. Yet, high doses are required to treat many diseases. To achieve these doses, most approved protein drug products are administered intravenously, imposing excessive burdens on patients and the healthcare system. New approaches are needed to formulate proteins at high concentrations to enable less burdensome subcutaneous injection, preferably in an autoinjector format. To address this challenge, we report a subcutaneously injectable biotherapeutic delivery platform composed of spray-dried protein microparticles suspended in a non-solvent liquid carrier. These microparticles contain only active biopharmaceutical agent and a high glass transition temperature polyacrylamide-derived copolymer excipient affording several key benefits over traditional excipients, including: (i) improved stabilization of biopharmaceuticals through the spray drying process, and (ii) improved morphology and properties of the spray-dried particles, enhancing suspension injectability. Experiments with albumin and human IgG demonstrate that this technology enables ultra-high-concentration protein formulations (exceeding 500 mg/mL) that are injectable through standard needles with clinically relevant injection forces. Additionally, experiments in mice show these ultra-high-concentration formulations reduce required injection volumes without altering pharmacokinetics. This approach could double the number of commercial protein drugs amenable to subcutaneous administration, dramatically reducing burden and improving access to these critical biopharmaceuticals.","PeriodicalId":501308,"journal":{"name":"bioRxiv - Bioengineering","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nicotine interacts with DNA lesions induced by alpha radiation which may contribute to erroneous repair in human lung epithelial cells 尼古丁与阿尔法辐射诱导的 DNA 损伤相互作用，可能导致人类肺上皮细胞的错误修复

bioRxiv - Bioengineering Pub Date : 2024-09-13 DOI: 10.1101/2024.09.07.611801

Nadia Boroumand

{"title":"Nicotine interacts with DNA lesions induced by alpha radiation which may contribute to erroneous repair in human lung epithelial cells","authors":"Nadia Boroumand","doi":"10.1101/2024.09.07.611801","DOIUrl":"https://doi.org/10.1101/2024.09.07.611801","url":null,"abstract":"Purpose: Epidemiological studies show that radon and cigarette smoke interact in inducing lung cancer, but the contribution of nicotine in response to alpha radiation emitted by radon is not well understood. Materials and methods: Bronchial epithelial BEAS 2B cells were either pre-treated with 2 uM nicotine during 16 h, exposed to radiation, or the combination. DNA damage, cellular and chromosomal alterations, oxidative stress as well as inflammatory responses were assessed to investigate the role of nicotine in modulating responses. Results: Less γH2AX foci were detected at 1 h after alpha radiation exposure (1 2 Gy) in the combination group versus alpha radiation alone, whereas nicotine alone had no effect. Comet assay showed less DNA breaks already just after combined exposure, supported by reduced p-ATM, p DNA PK, p p53 and RAD51 at 1 h, compared to alpha radiation alone. Yet the frequency of translocations was higher in the combination group at 27 h after irradiation. Although nicotine did not alter G2 arrest at 24 h, it assisted in cell cycle progression at 48 h post radiation. A slightly faster recovery was indicated in the combination group based on cell viability kinetics and viable cell counts, and significantly using colony formation assay. Pan-histone acetyl transferase inhibition using PU139 blocked the reduction in p p53 and γH2AX activation, suggesting a role for nicotine-induced histone acetylation in enabling rapid DNA repair. Nicotine had a modest effect on reactive oxygen species induction but tended to increase alpha particle induced pro inflammatory IL 6 and IL 1β (4 Gy). Interestingly, nicotine did not alter gamma radiation induced γH2AX foci.\u0000Conclusions: This study provides evidence that nicotine modulates alpha radiation response by causing a faster but more error prone repair, as well as rapid recovery, which may allow expansion of cells with genomic instabilities. These results hold implications for estimating radiation risk among nicotine users.","PeriodicalId":501308,"journal":{"name":"bioRxiv - Bioengineering","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards genuine three-dimensional diffusion imaging with physiological motion compensation 利用生理运动补偿实现真正的三维扩散成像

bioRxiv - Bioengineering Pub Date : 2024-09-13 DOI: 10.1101/2024.09.08.611927

Yishi Wang, Dehe Weng, Jieying Zhang, Tianyi Qian, Wenzhang Liu, Kun Zhou, Yanglei Wu, Baogui Zhang, Qing Li, Jing Jing, Zhe Zhang

{"title":"Towards genuine three-dimensional diffusion imaging with physiological motion compensation","authors":"Yishi Wang, Dehe Weng, Jieying Zhang, Tianyi Qian, Wenzhang Liu, Kun Zhou, Yanglei Wu, Baogui Zhang, Qing Li, Jing Jing, Zhe Zhang","doi":"10.1101/2024.09.08.611927","DOIUrl":"https://doi.org/10.1101/2024.09.08.611927","url":null,"abstract":"Purpose: We aim to implement a 3D DWI sequence and show its usage on patients with new ischemic lesions. Materials and Methods: The proposed 3D DWI sequence was implemented by integrating second-order gradient moment nulling (M2) and cardiac motion synchronization (Sync). All data were acquired on a 3T MAGNETOM Prisma scanner (Siemens Healthcare, Erlangen, Germany) using a 64 channel head and neck coil. 21 healthy volunteers underwent 3D DWI scans at 0.9 mm isotropic resolution using four motion compensation methods for comparison: no compensation (M0), M2 only, Sync only and the proposed M2+Sync method. 2D phase variation maps with different motion compensation methods were also acquired for one subject to illustrate the mechanism of the proposed method. A ghost-to-signal ratio (GSR) and blurring index was defined and compared among the four methods with repeated measures ANOVA and Tukey's test. 3D DWI was compared with 2D DWI for ADC quantification. Image quality and ischemic lesion conspicuity were evaluated with 12 patients after endovascular treatment. Results: Whole brain 3D DWI was achieved at 0.9 mm isotropic resolution within 5 minutes using the proposed sequence. M2+Sync achieved the lowest level of GSR and blurring along the slice direction. ADC quantification showed no statistically significant difference between M2+Sync compared to 2D DWI. 3D DWI showed similar image quality, higher lesion conspicuity and counts compared to 2D DWI. Conclusion: Direct 3D DWI can be achieved by the combination of second order gradient moment nulling and cardiac synchronization.","PeriodicalId":501308,"journal":{"name":"bioRxiv - Bioengineering","volume":"193 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142256041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0