Lei Yang, Anqi Di, Lishuang Song, Xuefei Liu, Di Wu, Song Wang, Zhengting Hao, Lige Bu, Chunling Bai, Guanghua Su, Zhuying Wei, Li Zhang, Zhonghua Liu, Shaorong Gao, Guangpeng Li
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引用次数: 0
摘要
单倍体雄性干细胞（haSCs）是研究动物性状的革命性资源，对家畜育种有重大影响，但家畜haSCs尚未获得。在这里，我们报告了利用新型 FACE 培养基衍生牛羊单倍体雄性干细胞的情况，这些细胞具有形成性多能特征。值得注意的是，将带有目标基因突变的原氨基化造血干细胞注射到卵母细胞中，可以有效地获得基因敲除的牛羊。
Rapid production of modified cattle and sheep from spermatid-like stem cells
Haploid androgenetic stem cells (haSCs) are revolutionary resources for studying animal traits and greatly impact livestock breeding, but livestock haSCs have yet to be obtained. Here, we report the derivation of cattle and sheep haSCs using a novel FACE medium, and these cells harbor formative pluripotency features. Notably, knockout cattle and sheep can be efficiently obtained upon injection of the protaminized haSCs with targeted gene mutations into oocytes.
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