随机定向微槽水凝胶引导细胞运动、调节速度并控制细胞扩散的方向性

Biswajoy Ghosh, Krishna Agarwal
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引用次数: 0

摘要

细胞迁移是一个基本的生物过程,但人们对细胞如何感知和驾驭复杂环境的机制仍然知之甚少。在这项研究中,我们开发了一种按细胞长度尺度设计的随机定向微沟槽系统,以探索响应不同地形的运动智能。这些微沟槽允许细胞自由选择迁移路径,揭示了细胞如何感知和适应拓扑线索的关键信息。利用在这些沟槽基底上迁移的成纤维细胞,我们研究了细胞过程,如肌动蛋白细胞骨架重塑、细胞粘附动力学以及沟槽排列对迁移速度和方向性的影响。我们的研究结果表明,细胞将其细胞骨架结构与沟槽几何形状对齐,形成富含肌动蛋白的锚,从而增强了在沟槽对齐环境中的迁移。与错位或对照条件下的细胞相比,在与其固有极性对齐的沟槽中迁移的细胞表现出更快、更有方向性的迁移。这项研究加深了我们对细胞-拓扑相互作用的理解,为组织工程在癌症治疗和伤口愈合中的应用提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Randomly oriented microgrooved hydrogel guides cellular motility, modulates speed, and governs directionality of cellular spread
Cell migration is a fundamental biological process, yet the mechanisms underlying how cells sense and navigate complex environments remain poorly understood. In this study, we developed a system of randomly oriented microgrooves, designed at cellular length scales, to explore motility intelligence in response to varied topographies. These microgrooves allowed cells to freely choose their migratory paths, revealing key insights into how cells sense and adapt to topological cues. Using fibroblast cells migrating over these grooved substrates, we examined cellular processes such as actin cytoskeleton remodeling, cell adhesion dynamics, and the impact of groove alignment on migration speed and directionality. Our results demonstrate that cells align their cytoskeletal structures to groove geometries, forming actin-rich anchors that enhance migration in groove-aligned environments. Cells migrating in grooves aligned with their intrinsic polarity exhibited faster, more directed migration compared to those in misaligned or control conditions. This work advances our understanding of cell-topology interaction and provides new perspectives for tissue engineering applications in cancer therapy and wound healing.
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