medRxiv - Urology最新文献_第3页

Acoustic enrichment of heterogenous circulating tumor cells and clusters from patients with metastatic prostate cancer 来自转移性前列腺癌患者的异质循环肿瘤细胞和肿瘤簇的声富集

medRxiv - Urology Pub Date : 2023-12-04 DOI: 10.1101/2023.12.04.23299128

Cecilia Magnusson, Per Augustsson, Eva Undvall Anand, Andreas Lenshof, Andreas Josefsson, Karin Welen, Anders Bjartell, Yvonne Ceder, Hans Lilja, Thomas Laurell

{"title":"Acoustic enrichment of heterogenous circulating tumor cells and clusters from patients with metastatic prostate cancer","authors":"Cecilia Magnusson, Per Augustsson, Eva Undvall Anand, Andreas Lenshof, Andreas Josefsson, Karin Welen, Anders Bjartell, Yvonne Ceder, Hans Lilja, Thomas Laurell","doi":"10.1101/2023.12.04.23299128","DOIUrl":"https://doi.org/10.1101/2023.12.04.23299128","url":null,"abstract":"Background: There are important unmet clinical needs to develop cell enrichment technologies to enable unbiased label-free isolation of both single cell and clusters of circulating tumor cells (CTCs) manifesting heterogeneous lineage specificity. Here, we report a pilot study based on microfluidic acoustophoresis enrichment of CTCs using the CellSearch CTC assay as a reference modality. Methods: Acoustophoresis uses an ultrasonic standing wave field to separate cells based on biomechanical properties (size, density, and compressibility) resulting in inherently label-free and epitope-independent cell enrichment. Following red blood cell lysis and paraformaldehyde fixation, 6 mL of whole blood from 12 patients with metastatic prostate cancer and 20 healthy controls were processed with acoustophoresis and subsequent image cytometry. Results: Acoustophoresis enabled enrichment and characterization of phenotypic CTCs (EpCAM+, Cytokeratin+, DAPI+, CD45-/CD66b-) in all patients with metastatic prostate cancer and detected CTC-clusters composed of only CTCs or heterogenous aggregates of CTCs clustered with various types of white blood cells in 9 out of 12 patients. By contrast, CellSearch did not detect any CTC-clusters, but detected comparable numbers of phenotypic CTCs as acoustophoresis, with trends of finding higher number of CTCs using acoustophoresis. Conclusion: Our preliminary data indicate that acoustophoresis provides excellent possibilities to detect and characterize CTC-clusters as a putative marker of metastatic disease and outcomes. Moreover, acoustophoresis enables sensitive label-free enrichment of cells with epithelial phenotype in blood and offers opportunities to detect and characterize CTCs undergoing epithelial-to-mesenchymal transitioning and lineage plasticity.","PeriodicalId":501140,"journal":{"name":"medRxiv - Urology","volume":"104 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138510429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Spatial Comparison of Molecular Features Associated with Resistance to Pembrolizumab in BCG Unresponsive Bladder Cancer 卡介苗无应答性膀胱癌患者派姆单抗耐药相关分子特征的空间比较

medRxiv - Urology Pub Date : 2023-11-30 DOI: 10.1101/2023.11.28.23299093

Khyati Meghani, Noah Frydenlund, Yanni Yu, Bonnie Choy, Joshua J. Meeks

引用次数: 0

Identifying patients with rapid progression from hormone-sensitive to castration-resistant prostate cancer: a retrospective study 鉴别从激素敏感到去势抵抗性前列腺癌快速进展的患者:一项回顾性研究

medRxiv - Urology Pub Date : 2022-10-24 DOI: 10.1101/2022.10.23.22281406

Chenxi Pan, YI He, He Wang, Yang Yu, Lu Li, Lingling Huang, Mengge Lv, Weigang Ge, Bo Yang, Yaoting Sun, Tiannan Guo, Zhiyu Liu

{"title":"Identifying patients with rapid progression from hormone-sensitive to castration-resistant prostate cancer: a retrospective study","authors":"Chenxi Pan, YI He, He Wang, Yang Yu, Lu Li, Lingling Huang, Mengge Lv, Weigang Ge, Bo Yang, Yaoting Sun, Tiannan Guo, Zhiyu Liu","doi":"10.1101/2022.10.23.22281406","DOIUrl":"https://doi.org/10.1101/2022.10.23.22281406","url":null,"abstract":"Background: Prostate cancer (PCa) is the second most prevalent malignancy and the fifth cause of cancer-related deaths in men. A crucial challenge is identifying the population at risk of rapid progression from hormone-sensitive PCa (HSPC) to the lethal castration-resistant PCa (CRPC).\u0000Methods: We collected 78 HSPC biopsies and measured their proteomes using pressure cycling technology and a pulsed data-independent acquisition pipeline. The proteomics data and clinical metadata were used to generate models for classifying HSPC patients and predicting the development of each case.\u0000Results: We quantified 7,961 proteins using the HSPC biopsies. A total of 306 proteins were differentially expressed between patients with a long- or short-term progression to CRPC. Using a random forest model, we identified ten proteins that significantly discriminated long- from short-term cases, which were used to classify PCa patients with an 86% accuracy. Next, two clinical parameters (Gleason sum and total PSA) and five proteins (DPT, ARGEF1, UTP23, CMAS, and ANAPC4) were found to be significantly associated with rapid disease progression. A nomogram model using these seven features was generated for stratifying patients into groups with significant progression disparities.\u0000Conclusion: We identified proteins associated with a fast progression to CRPC and an unfavorable prognosis. Based on these proteins, our machine learning and nomogram models stratified HSPC into high- and low-risk groups and predict their prognoses. These tools may aid clinicians in predicting the progression of patients, guiding individualized clinical management and decisions.","PeriodicalId":501140,"journal":{"name":"medRxiv - Urology","volume":"106 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138510428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound versus fluoroscopy as imaging guidance for percutaneous nephrolithotomy: A systematic review and meta-analysis 超声与透视作为经皮肾镜取石术的影像学指导:一项系统回顾和荟萃分析

medRxiv - Urology Pub Date : 2022-10-13 DOI: 10.1101/2022.10.13.22281046

Razman Arabzadeh Bahri, Saba Maleki, Arman Shafiee, Parnian Shobeiri

{"title":"Ultrasound versus fluoroscopy as imaging guidance for percutaneous nephrolithotomy: A systematic review and meta-analysis","authors":"Razman Arabzadeh Bahri, Saba Maleki, Arman Shafiee, Parnian Shobeiri","doi":"10.1101/2022.10.13.22281046","DOIUrl":"https://doi.org/10.1101/2022.10.13.22281046","url":null,"abstract":"Objectives: To determine whether the outcomes of ultrasound-guided percutaneous nephrolithotomy (UG-PCNL), an alternative to traditional fluoroscopy-guided percutaneous nephrolithotomy (FG-PCNL), are comparable.\u0000Methods: A systematic search of PubMed, Embase, and the Cochrane Library was carried out to discover investigations comparing UG-PCNL to FG-PCNL, and accordingly, a meta-analysis of those studies was performed. The primary outcomes included the stone-free rate (SFR), overall complications based on Clavien-Dindo classification, duration of surgery, duration of patients’ hospitalization, and hemoglobin (Hb) drop during the surgery. All statistical analyses and visualizations were implemented utilizing R software.\u0000Results: Nineteen studies, including eight randomized clinical trials (RCTs) and eleven observational cohorts, comprising 3016 patients (1521 UG-PCNL patients) and comparing UG-PCNL with FG-PCNL met the inclusion criteria of the current study. Considering SFR, overall complications, duration of surgery, duration of hospitalization, and Hb drop, our meta-analysis revealed no statistically significant difference between UG-PCNL and FG-PCNL patients, with p-values of 0.29, 0.47, 0.98, 0.28, and 0.42, respectively. Significant differences were discovered between UG-PCNL and FG-PCNL patients in terms of the length of time they were exposed to radiation (p-value< 0.0001). Moreover, FG-PCNL had shorter access time than UG-PCNL (p-value= 0.04).\u0000Conclusion: UG-PCNL provides the advantage of requiring less radiation exposure while being just as efficient as FG-PCNL; thus, this study suggests prioritizing the use of UG-PCNL.","PeriodicalId":501140,"journal":{"name":"medRxiv - Urology","volume":"88 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138510430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of COVID-19 on the management and outcomes of ureteric stones in the UK: a multicentre retrospective study COVID-19对英国输尿管结石治疗和结局的影响:一项多中心回顾性研究

medRxiv - Urology Pub Date : 2022-06-28 DOI: 10.1101/2022.06.27.22276955

Matthew H V Byrne, Fanourios Georgiades, Alexander Light, Catherine E Lovegrove, Catherine Dominic, Josephine Rahman, Senthooran Kathiravelupillai, Tobias Klatte, Kasra Saeb-Parsy, Rajeev Kumar, Sarah Howles, Grant D Stewart, Ben Turney, Oliver Wiseman, the COVID Stones Collaborative

引用次数: 0

The expression of cancer stem cells and its effects on the propensity for recurrence and metastasis in bladder cancer: a systematic review 肿瘤干细胞的表达及其对膀胱癌复发和转移倾向的影响:系统综述

medRxiv - Urology Pub Date : 2022-05-19 DOI: 10.1101/2022.05.18.22275252

Agus Rizal Ardy Hariandy Hamid, Yasmina Zahra Syadza, Oliver Emmanuel Yausep, Roberto Bagaskara Indy Christanto, Bayu Hernawan Rahmat Muharia, Chaidir Arif Mochtar

{"title":"The expression of cancer stem cells and its effects on the propensity for recurrence and metastasis in bladder cancer: a systematic review","authors":"Agus Rizal Ardy Hariandy Hamid, Yasmina Zahra Syadza, Oliver Emmanuel Yausep, Roberto Bagaskara Indy Christanto, Bayu Hernawan Rahmat Muharia, Chaidir Arif Mochtar","doi":"10.1101/2022.05.18.22275252","DOIUrl":"https://doi.org/10.1101/2022.05.18.22275252","url":null,"abstract":"Bladder cancer is one of the most frequent cancers of the urinary tract, associated with high recurrence rates and metastasis. Cancer stem cells (CSCs) are a subpopulation of cancer cells characterized by high self-renewal and differentiation capacities, resulting in increased cancer recurrence, larger tumor size, higher rates of metastasis, higher resistance to treatment, and overall poorer prognosis. This study aimed to evaluate the role of CSCs as a prognostic tool to predict the risks of metastasis and recurrence in bladder cancer. A literature search was conducted across seven databases from January 2000 to February 2022 for clinical studies investigating the use of CSCs to determine the prognosis of bladder cancer. The following keywords were used: (“Bladder Cancer” OR “Transitional Cell Carcinoma” OR “Urothelial Carcinoma”) AND (“Stem Cell” OR “Stem Gene”) AND (“Metastasis” OR “Recurrence”). A total of 12 studies were deemed eligible for inclusion. SOX2, IGF1R, SOX4, ALDH1, CD44, Cripto-1, OCT4, ARRB1, ARRB2, p-TFCP2L1, CDK1, DCLK1, and NANOG, which were all identified as CSC markers, have been implicated in the recurrence and metastasis of tumor in bladder cancer, which played a role as prognostic factor of bladder cancer. Given the pluripotent and highly proliferative properties of CSCs. CSCs may play a role in the complex biological behavior of bladder cancer, including, but not limited to, its high rates of recurrence, metastasis, and resistance to treatment. The detection of cancer stem cell markers offers a promising approach in determining the prognosis of bladder cancer. Further studies in this area are thus warranted and may contribute significantly to the overall management of bladder cancer.Cancer stem cells; Bladder cancer; Recurrency of bladder cancer; Metastasis of bladder cancer; Prognosis factor of bladder cancer","PeriodicalId":501140,"journal":{"name":"medRxiv - Urology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138537729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraoperative calculus or hemorrhage in transurethral seminal vesiculoscopy as a risk factor for recurrent hemospermia 经尿道精囊镜术中结石或出血是复发性血精症的危险因素

medRxiv - Urology Pub Date : 2022-04-29 DOI: 10.1101/2022.04.28.22274431

Cheng-En Mei, Ju-Chuan Hu, Jian-Ri Li, Kun-Yuan Chiu, Shian-Shiang Wang, Chuan-Shu Chen

引用次数: 0

Glucose and lipid profiles in men with non-obstructive azoospermia 非阻塞性无精子症患者的葡萄糖和脂质谱

medRxiv - Urology Pub Date : 2022-03-15 DOI: 10.1101/2022.03.14.22272336

Ponco Birowo, Dimas Tri Prasetyo, Dwi Ari Pujianto, Widi Atmoko, Nur Rasyid

{"title":"Glucose and lipid profiles in men with non-obstructive azoospermia","authors":"Ponco Birowo, Dimas Tri Prasetyo, Dwi Ari Pujianto, Widi Atmoko, Nur Rasyid","doi":"10.1101/2022.03.14.22272336","DOIUrl":"https://doi.org/10.1101/2022.03.14.22272336","url":null,"abstract":"The impact of a low testosterone level among men with non-obstructive azoospermia with various testicular histopathological patterns on the regulation of glucose and lipid metabolism is less well known than among the general population. The aim of this retrospective study was to examine the association between testicular histopathology and components of the metabolic profile among men with non-obstructive azoospermia. Participants were divided into two groups: men with non-obstructive azoospermia and men with obstructive azoospermia. Testicular biopsies were performed among those with non-obstructive azoospermia. We included 115 patients in this study: 83 (72.2%) had non-obstructive azoospermia and 32 (27.8%) had obstructive azoospermia. The plasma glucose concentration, glycated hemoglobin level, and lipid profile were similar between patients with non-obstructive azoospermia and those with obstructive azoospermia. Upon subgroup analysis of patients with non-obstructive azoospermia, those with Sertoli-cell-only syndrome had the lowest testosterone (431 ± 238 ng/dL; P=0.039) and highest follicle-stimulating hormone (23.4 ± 18.2 mIU/mL; P=0.002) concentrations. They also had the highest triglyceride concentration (163 ± 114 mg/dL; P=0.001). Interestingly, patients with Sertoli-cell-only syndrome had a lower fasting plasma glucose concentration (92 ± 11 mg/dL; P<0.001) and glycated hemoglobin level (5.9 ± 0.8%; P=0.022) than those with histopathological patterns of maturation arrest and hypospermatogenesis. In conclusion, differences in glucose and lipid metabolism are evident between men with non-obstructive azoospermia with different spermatogenesis patterns.","PeriodicalId":501140,"journal":{"name":"medRxiv - Urology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138537718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0